title stringlengths 0 901 | abstract stringlengths 3 9.89k | PMID int64 22 25.3M | embedding listlengths 768 768 |
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Role of acetate metabolism in sporulation of Saccharomyces carlsbergensis. | Several aspects of the role of acetate metabolism in the sporulation of Saccaromyces carlsbergensis were investigated. Experiments in which the development of the respiratory system was either stimulated by growth on sugars to which the cells have to adapt, or inhibited by chloramphenicol suggested a correlation betwee... | 2,101 | [
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The influence of physicochemical factors on the thermal inactivation of murine interferon. | The degradation of biological activity of virus-induced murine interferon was determined in linear nonisothermal and multiple isothermal tests. The stabilizing effect of pH during heating on interferon in solution was greatest at low pH, such that pH 2 greater than pH 5 greater than pH 7 greater than or equal to pH 9; ... | 2,143 | [
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Factors regulating amino acid release from extrasplanchnic tissues in the rat. Interactions of alanine and glutamine. | 1. Factors regulating the release of alanine and glutamine in vivo were investigated in starved rats by removing the liver from the circulation and monitoring blood metabolite changes for 30 min. 2. Alanine and glutamine were the predominant amino acids released into the circulation in this preparation. 3. Dichloroacet... | 2,155 | [
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Fructose 1,6-diphosphate aldolase from rabbit muscle. Effect of pH on the rate of formation and on the equilibrium concentration of the carbanion intermediate. | The rate of oxidation of ferricyanide of the aldolase-dihydroxyacetone phosphate complex was measured under different conditions. The following conclusions are drawn. 1. In the cleavage of fructose diphosphate, catalysed by native aldolase, the steady-state concentration of the enzyme-dihydroxyacetone phosphate carbani... | 2,160 | [
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Purification and properties of the thermostable acid protease of Penicillium duponti. | An acid protease produced by the thermophilic fungus Penicillium duponti K 1014 has been purified by consecutive ion-exchange and gel permeation chromatography, and crystallized from aqueous acetone solution. The purified endopeptidase gave a symmetrical schlieren peak by sedimentation velocity, and was found to be hom... | 2,287 | [
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Avoidance of strongly chaotropic eluents for immunoaffinity chromatography by chemical modification of immobilized ligand. | The need for chaotropic eluents in immunoaffinity chromatography is a consequence of the high affinities of antibodies towards their antigens. This affinity is decreased and elution of antiglucagon antibodies from a column of immobilized glucagon can be achieved under mild conditions when the steric complementarity to ... | 2,298 | [
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Purification and some properties of rat liver cysteine oxidase (cysteine dioxygenase). | Cysteine oxidase (cysteine dioxygenase, EC 1.13.11.20) was purified approximately 1000-fold from rat liver. The purified enzyme (protein-B) was obtained as an inactive form, which was activated by anaerobic preincubation with L-cysteine. The active form of protein-B was inactivated during aerobic incubation to produce ... | 2,307 | [
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Separation of subchloroplast membrane particles by counter-current distribution. | Counter-current distribution in an aqueous Dextran-polyethylene glycol two-phase system has been used to fractionate membrane fragments obtained by press treatment of Class II chloroplasts. By the counter-current distribution technique membrane particles are separated according to their surface properties such as charg... | 2,312 | [
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Relations between the electrical potential, pH gradient, proton flux and phosphorylation in the photosynthetic membrane. | The transmembrane electrical potential (deltaphi), the proton flux (H+), the rate of electron transport (e), the pH gradient (deltapH) and the rate of phosphorylation (ATP) were measured in chloroplasts of spinach. Photosynthesis was excited periodically with flashes of variable frequencies and intensities. A new metho... | 2,316 | [
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Primary reactions of photosystem II at low pH. I. Prompt and delayed fluorescence. | Prompt and delayed chlorophyll fluorescence have been studied in broken spinach chloroplasts at pH values down to 2.6. No direct effect of low pH on the primary charge separation in Photosystem II was observed. The irreversible inactivation of a secondary electron donor in a narrow pH range around pH 4.5 was demonstrat... | 2,318 | [
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The rate of calcium uptake into sarcoplasmic reticulum of cardiac muscle and skeletal muscle. Effects of cyclic AMP-dependent protein kinase and phosphorylase b kinase. | Calcium transport into sarcoplasmic reticulum fragments isolated from dog cardiac and mixed skeletal muscle (quadriceps) and from mixed fast (tibialis), pure fast (caudofemoralis) and pure slow (soleus) skeletal muscles from the cat was studied. Cyclic AMP-dependent protein kinase and phosphorylase b kinase stimulated ... | 2,325 | [
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[Effect of IAA on the photophosphorylation of pea isolated chloroplasts]. | Effect of IAA (10(-10)-10(-3) M) on photophosphorylation, NADP reduction and the oxygen exchange is investigated. It is shown that low concentrations of IAA (10(-10)-10(-7) M) increase the photophosphorylation reaction and the flow of electrones to NADP under the phosphorylation conditions in the chloroplasts, and thei... | 2,332 | [
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[Study of the osmotic behaviour of human platelets]. | Observation of the behaviour of platelets in hypotonic media affords an approach to the evaluation of their fragility. It should be possible to apply such a test to various fields such as the study of rheological properties of platelets, the investigation of certain pathological cases and eventual alterations during st... | 2,344 | [
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[Rapid production of a biotin deficiency in mice]. | This work describes a rapid method for the production of a biotin-deficiency in mice. Besides classical morphological symptoms, a decrease of activities of some biotin-dependent enzymes was also observed. The biotin-enzymes are not inhibited at the same extent in a same organ and the metabolic changes do not always fol... | 2,375 | [
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Studies on fazadinium bromide (ah 8165): a new non-depolarizing neuromuscular blocking agent. | Intravenous dose-response relationships were used to correlate neuromuscular paralysis with the effects of fazadinium (AH 8165) on autonomic mechanisms in anaesthetized cats and rhesus monkeys and with cardiovascular effects in man. In cats and monkeys neuromuscular paralysis of the twitch responses of the gastrocnemiu... | 2,378 | [
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Laboratory aids to diagnosis--enzymes. | A summary is presented of those organ specific enzyme assays traditionally used in evaluation of the patient with cancer. In addition, the use of certain serum enzymes such as gamma-glutamyl transpeptidase, phosphohexose isomerase or 5'-nucleotidase as aids in following the course of the disease, particularly in patien... | 2,380 | [
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Failure to monoaminergic and cholinergic receptor blockers to prevent prostaglandin E2-induced luteinizing hormone release. | Receptor blocking drugs were used to determine whether adrenergic, dopaminergic, serotoninergic, or cholinergic synapses are involved in mediating the LH release induced by intraventricularly injected PGE2. Prostaglandin E2 (5mug) was injected into the 3rd ventricle (3rd V) of ovariectomized rats, and plasma LH concent... | 2,457 | [
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Influence of unsaturated fatty acids in chloroplasts. Shift of the pH optimum of electron flow and relations to deltapH, thylakoid internal pH and proton uptake. | Linolenic acid (C18:3) is the main endogenous unsaturated fatty acid of thylakoid membrane lipids, and seems in its free form to exert significant effects on the structure and function of photosynthetic membranes. In this investigation the effect of linolenic acid was studied at various pH values on the electron flow r... | 2,470 | [
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Effects on rectal temperature in rats of gamma-aminobutyric acid; possible mediation through putative transmitters. | The rectal temperature of male rats was measured in a thermoneutral environment (25 degrees C) and at ambient temperatures of 15 and 35 degrees C. Unless otherwise specified all drugs were administered intracerebroventricularly (i.c.v.) and all results are reported for the thermoneutral environment. Exposure to 15 degr... | 2,482 | [
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Properties of glutamine aminohydrolases in subcellular fractions of liver of tumour bearing mice. | Glutamine aminohydrolase is found to be present in microsomal and soluble supernatant in liver of EAC-bearing mice. Enzymes obtained from these two sources were characterized and found to behave differently from the mitochondrial glutaminase of both normal and tumour-bearing mice. | 2,490 | [
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The alternate complement pathway in inflammatory bowel disease. Quantitation of the C3 proactivator (factor B) protein. | A component of the complement system's alternate pathway was investigated in ulcerative colitis and Crohn's disease. The mean C3PA (Factor B) titer in normals was 74 +/- 15%; in ulcerative colitis, 92 +/- 18%; and in Crohn's disease, 119+/- 24%. Significance was at the P less than 0.001 level when the mean values for t... | 2,509 | [
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A histochemical study of the apparent deamination of proteins by sodium hypochlorite. | The possible chemical mechanisms by which neutral solutions of sodium hypochlorite containing a high concentration of sodium chloride abolish the acidophilia of proteins in sections of fixed tissue are reviewed. The most probable one is the chlorination of the protein terminal amino groups, followed by the breakdown of... | 2,567 | [
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The possibilities and limitations of membrane methods for the histochemical demonstration of cholinesterases. | The thiocholine method for the histochemical detection of cholinesterases according to Karnovsky-Roots was adapted for unfixed cryostat sections by addition of the agar solution to the incubation mixture and by using the semipermeable membrane interposed between the section and the incubation medium. The procedure prev... | 2,569 | [
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Collaborative study of the Food Chemicals Codex method for the determination of the neutralizing value of sodium aluminum phosphate. | Fifteen laboratories participated in a collaborative study to evaluate the Food Chemicals Codex method for the determination of the neutralizing value of sodium aluminum phosphate. The AOAC method for determining the neutralizing value of sodium acid pyrophosphate, sec. 8.010, was also included in the study. The precis... | 2,581 | [
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Inhibition by superoxide dismutase of methemoglobin formation from oxyhemoglobin. | The formation of methemoglobin from oxyhemoglobin in a solution containing photoreduced riboflavin and oxygen was inhibited by superoxide dismutase. The rate of the reaction was pH-dependent in the range of 6.8 to 7.8, increasing as the pH was reduced. Inhibition by superoxide dismutase was enhanced as the EDTA concent... | 2,597 | [
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The fluorimetric detection of pesticides on aluminium oxide layers. | Several pesticides have been investigated for their fluorigenic properties on acidic and basic aluminium oxide layers. Fluorescence was obtained in several instances and the relative intensities were observed. Fluorescence spectra were recorded for the best fluorescence obtained before and after heat treatment of the c... | 2,611 | [
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A comparison of female and male neurotic depressives. | Female and male neurotic depressives in an outpatient psychiatric clinic were compared on measures of subjective distress, interpersonal concerns, and types of treatment given. There were essentially no differences between the two groups on the patient self-report measures. However, female patients were found to have s... | 2,620 | [
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Lymphoid cell fractionation by aggregated immunoglobulin-agarose columns. | Fractionation by columns of aggregated rat immunoglobulin (Agg Ig)-agarose was investigated as a method of separating different populations of lymphoid cells. With rat spleen cells, Agg Ig columns retained phagocytes, IgM- and IgG-antibody-forming-cells, cells mediating antibody- or PHA-induced lysis of chicken erythro... | 2,637 | [
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... |
Detection of pneumococcal polysaccharide in the sputum of patients with pneumococcal pneumonia by counterimmunoelectrophoresis. | Each of 41 patients with bacterial pneumonia was placed into 1 of 4 categories based on the relative clinical certainty of the diagnosis of pneumococcal pneumonia. The frequency of pneumococcal polysaccharide in the sputum by counterimmunoelectrophoresis (CIE) was then noted for each diagnostic category of patients. De... | 2,641 | [
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Cerebral monoamine metabolism in guinea-pigs with ascorbic acid deficiency. | Guinea-pigs kept on a diet deficient in vitamin C showed, after 3 weeks, a marked decrease of ascorbic acid in brain and blood leucocytes as well as of the activity of alkaline phosphatase in blood plasma. Pair-fed animals did not exhibit these changes. The alpha-methyl-p-tyrosine (alpha MpT)-induced diminution of nora... | 2,659 | [
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A study of the human metabolism of secbutobarbitone. | The urinary excretion of secbutobarbitone (I) and its metabolites has been studied quantitatively using combined gas chromatography-mass spectrometry. After a single oral dose was given to healthy male volunteers, unchanged drug (5-9%), 2'-hydroxysecbutobarbitone (II, 1.7-3.2%), 2'-oxosecbutobarbitone (III, less than 1... | 2,664 | [
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[Effect of a magnetic field on Escherichia coli]. | The decontaminating effect of the pulsing magnetic field on the E. coli infected reclaimed water was studied on two installations. The magnetic field intensity was 500 and 1000-1500 ersted and the microbial load was 1, 10 and 100 thous. microbial units per 1 ml. It was found that the magnetic treatment of water had a n... | 2,806 | [
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Blood alcohol level discrimination by nonalcoholics. The role of internal and external cues. | Men were trained to estimate their blood alcohol levels, after drinks of different strengths, by means of internal or external cues or both. All groups improved in estimation accuracy but the type of training made no difference. | 2,811 | [
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Differential inhibition of sclerotial germination in Whetzelinia sclerotiorum. | A number of diverse compounds including divalent metal ions, simple sugars, and common counterions, buffers, and fungicides were surveyed in the laboratory with regard to ability to inhibit germination of field-collected sclerotia from Whetzelinia sclerotiorum. Many compounds were inhibitory and several were comparable... | 2,873 | [
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Cobalamins in fibroblasts cultured from normal control subjects and patients with methylmalonic aciduria. | The intracellular content and proportional distribution of B12 (cobalamin) derivatives in fibroblasts cultured from patients with various forms of methylmalonic aciduria, as well as from normal control subjects, has been determined by a two-dimensional chromatobioautographic technique. Each line of fibroblasts was grow... | 2,896 | [
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Formation and in-vivo-distribution of different 99mTc-Sn-pyrophosphate complexes. | The formation of two different 99mTc-Sn-pyrophosphate complexes was determined by evaluation of their in vivo distribution in rats. It was found that formation of a 2 : 2 Sn : PyP complex, which is bone seeking, is depending as well on the pyrophosphate as on the hydrogen ion concentration in the equilibrium. On the co... | 2,905 | [
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... |
Separation of (3') deoxynucleotides with cation exchange columns. | A procedure is described for a one-step separation of synthetic (3') deoxynucleotides purchased commercially or those purified and isolated from the enzymatic digests of DNA. The method is simple. A 50 ml buret was used for column which was filled with the resin slurry and packed at water aspirator pressure. The compou... | 2,911 | [
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Force development of isoproterenol-damaged frog heart muscle in cyanide anoxia. | From recent experiments it is indicated that in frogs living at +25 degrees C the abnormal response to cyanide anoxia appears as soon as 4-5 hr after the first isoproterenol (IPR) injection, but the aneurysm does not appear until a few hours after the second injection of IPR. There is evidently a discrepancy between th... | 2,958 | [
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[The clinical importance of angiography in the diagnosis of periarteritis nodosa]. | Report on a 53 years old patient with renal failure. Angiography showed multiple micro-aneurysms in kidneys and liver, in some instances also in the pancreatic, duodenal and mesenterial arteries. Muscle biopsy confirmed the suspicion of arteriitis nodosa. Clinically the diagnosis of necrotizing arteriitis is difficult.... | 2,965 | [
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0.004095339,... |
[Proceedings: Home treatment of chronic respiratory insufficiency: inhalation of beta stimulators]. | In the author's experience, the longterm treatment of chronic obstructive lung disease should be supplemented by continuous selective stimulation of the adrenergic beta-receptors in the smooth bronchial muscles. The following procedure is recommended: inhalation of 15 min 3 times daily using an adequate aerosol with a ... | 2,970 | [
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0.076505... |
[Age-related properties of inductive synthesis of glucose-6-phosphatase, fructose-1,6-diphosphatase, tyrosine aminotransferase and tryptophan pyrrolase in hypothalamus stimulation]. | In aged rats electrostimulation of hypothalamus caused less distinct induction of the enzymes in liver tissue, kidney and spleen as compared with the adult animals. The activation of the total RNA synthesis (actinomycin D and olivomycin) eliminated the alterations in the enzymatic activities, caused by the stimulation ... | 3,019 | [
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[An improved trihydroxyindole method for the determination of urinary catecholamines]. | Commercially available columns for ion exchange chromatography were used for the separation of catecholamines in urine. The estimation of catecholamines was performed fluorimetrically by a new trihydroxyindole method. The fluorescence of adrenaline or noradrenaline was enhanced, in comparison with other methods, by the... | 3,040 | [
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Studies on fragments of rod outer segments from bovine retinas. | Monolayers of fragments of our rod segments were formed at an air-water interface. The area of these particles was measured as a function of the pH of the aqueous phase. A maximum area was measured at pH 6.5. The film was characterised by measuring the decrease in surface pressure, after compression, and the half time ... | 3,051 | [
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Effects of acute metabolic acidosis on parathyroid hormone action and calcium mobilization. | Mechanisms through which metabolic acidosis increases calcium mobilization have been investigated in thyroparathyroidectomized rats with induction of acute metabolic acidosis by infusing NH4C1 intravenously. Acute metabolic acidosis directly raised serum calcium concentration and augmented the effect of parathyroid hor... | 3,111 | [
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Metabolism of histamine in secreting and isolated canine stomach. | Total 14C activity in juice secreted by gastric pouches of six dogs and seven isolated canine stomachs was determined in response to intravenous and intra-arterial infusions of histamine and [14C]histamine. The proportions of 14C attributable to histamine, Nalpha-methylhistamine (NalphaMeH), Nalpha,Nalpha-dimethylhista... | 3,112 | [
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[Comparative study of neuraminidases from "Diplococcus pneumoniae" and "Clostridium perfringens"]. | Neuraminidases have been purified from the culture medium of two microorganisms, one aerobic, Diplococcus neumoniae, the other anaerobic, Clostridium perfringens. The enzymatic properties of the 2 neuraminidases have been studied (pH optimum; effect of cations; activity toward different substrates: neuraminyllactose, d... | 3,129 | [
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... |
A reporter group delivery system with both absolute and selective specificity for thiol groups and an improved fluorescent probe containing the 7-nitrobenzo-2-oxa-1,3-diazole moiety. | 1. 4-(N-2-Aminoethyl2'-pyridyl disulphide)-7-nitrobenzo-2-oxa-1,3-diazole (compound I) was synthesized and evaluated as a fluorescent labelling reagent for thiol groups. 2. The design of compound (I) as one example of a general type of reporter group delivery reagent (2-pyridyl-S-S-X, where X contains an environmentall... | 3,168 | [
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[Analytical profile of purified hexetidine (author's transl)]. | Physico-chemical, spectroscopic (UV, IR, NMR, mass), chromatographic (GLC, TLC) properties and synthesis of 1,3-bis(2-ethylhexyl)-5-amino-5-methyl-hexahydropyrimidine (hexetidine) are reported and discussed. Moreover, the difference between commercial and purified hexetidine is demonstrated. | 3,183 | [
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[On the influence of a special preparation of oxytetracycline and sodiumbituminosulfonates on amount and composition of skin surface lipids in acne vulgaris (author's transl)]. | Two groups of 27 and 23 patients with acne vulgaris were first treated for a period of one week with 1 g oxytetracycline a day p.o. In a second treatment period of 6 weeks the first group received 100 mg oxytetracycline a day p.o. and the second group a combination of 100 mg oxytetracycline and 1.2 g sodiumbituminosulf... | 3,187 | [
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[Influence of ethanol on the in vitro and in vivo drug release from some sustained release tablets (author's transl)]. | The in vitro and in vivo liberation of acetylsalicylic acid from sustained release tablets in presence of ethanol is described. Simultaneous uptake of 120 ml commercial brandy resulted in a faster release of the active substance from the tablets prepared with Eudragit ret-l (PM), as has been proved by urinary excretion... | 3,188 | [
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Changes in liver function after different types of surgery. | Liver function tests carried out after minor surgical procedures, under anaesthesia lasting for 1 hr, showed no abnormalities. Tests after body surface operations under the same anaesthetic techniques showed transient derangements. After intra-abdominal procedures, liver dysfunction was more marked, although no patient... | 3,192 | [
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Studies on the energy metabolism in lichen planus. | Various epidermal enzymes and cofactors were measured in patients with lichen planus and in healthy controls with the aid of Lowry's microtechniques, including enzymatic cycling. The steady-state levels of the nicotinamide adenine dinucleotides NAD and NADP were decreased and this was evident even in areas still free f... | 3,193 | [
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A kinetic study of protein-protein interactions. | Kinetic studies have been carried out of the monomer-dimer interaction of insulin, beta-lactoglobulin, and alpha-chymotrypsin using stopped-flow and temperature-jump techniques. The pH indicators bromothymol blue, bromophenol blue, and phenol red were used to monitor pH changes associated with the monomer-dimer interac... | 3,211 | [
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Studies on some lipogenic enzymes of cultured myeloid leukemic cells. | The microsomal fraction of M1 cells (an established cell line of myeloid leukemia) was capable of catalyzing acylation of sn-glycerol 3-phosphate by long-chain fatty acyl-CoA thioesters. The principal lipid product formed was identified as phosphatidic acid. Palmityl-CoA, stearyl-CoA, and oleyl-CoA were more effective ... | 3,240 | [
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The expectation of outcome from maintenance therapy in chronic schizophrenic patients. | The results from a prospective follow-up study of a group of schizophrenic patients suggest that a significant proportion (41 per cent) are likely to relapse during a two-year period despite the prescription of long-acting injectable neuroleptic drugs. Some will relapse because of a failure of the regime, but others (3... | 3,251 | [
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0.04572379,
... |
New techniques for ion-selective measurements of ionized calcium in serum after pH adjustment of aerobically handled sera. | I report further experience in measuring ionized calcium (Ca2+) with the AMT Electron System and its serum standards and solid-state, dip, calcium-selective electrodes. With this system, serum pH can be adjusted with CO2 gas and Ca2+ and pH simultaneously measured; when 5.2% CO2 (40 mm pco2) is used for sample equilibr... | 3,297 | [
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Elastin--proteoglycan interaction. Conformational changes of alpha-elastin induced by the interaction. | The interaction between alpha-elastin and a connective tissue proteoglycan was followed by optical density measurements and circular dichroism spectroscopy. It was found that interaction takes place at pH values below the isoelectric point of elastin with the formation of a complex coacervate. CD spectra demonstrated c... | 3,386 | [
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The influence of dehydrocholate on hepatic uptake and biliary excretion of 3H-taurocholate and 3H-ouabain. | The hepatic uptake and biliary excretion of 3H-taurocholate and 3H-ouabain was studied in the rat during saline (control) and dehydrocholate infusions. Dehydrocholate (140 mumol/hr) did not influence the plasma disappearance nor the biliary excretion of taurocholate after a single iv injection (37 mumol/kg). Bile produ... | 3,395 | [
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... |
Physiological disposition and metabolism of N-t-butylarterenol and its di-p-toluate ester (bitolterol) in the rat. | The metabolism and disposition of the bronchodilator, N-t-butylarterenol (tBA) and its di-p-toluate ester (bitolterol) were compared in the rat. Radioactivity was preferentially retained in lungs of rats compared with heart and blood after iv medication with tritium-labeled bitolterol, but was not retained in tissues a... | 3,403 | [
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[Responses of small intestine tissue chemoreceptors to change in the pCO2, pH and (HCO3-) in perfusion solutions]. | Perfusion of the small intestine of anesthetized cats with a solution having excessive CO2 and H+ concentration (pCO2 60 mm Hg; pH; 7.2; [HCO3-] 25 MM) produced a threshold reflex increase in the blood pressure. The subsequent increase of pCO2 to 380 mm Hg and decrease of pH to 6.4 evoked a gradual raise of the blood p... | 3,446 | [
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Post-prandial changes in PH and electrolyte concentration, in the upper jejunum after truncal vagotomy and drainage in man. | The changes in pH and concentration of electrolytes in the jejunal lumen after a hypertonic fluid meal have been studied after truncal vagotomy and drainage, with and without diarrhoea. The results show that, in these respects, there are no specific changes in the jejunal content associated with post-vagotomy diarrhoea... | 3,456 | [
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0.42... |
[Conditions for the efficacy of nutritional therapy]. | This is to discuss regulatory mechanisms which enable the organism to respond optimally to special nutritive conditions. Those regulatory mechanisms are often in disorder with patients who need nutritive therapy. However, normal functions of the body are required for nutrition. Disarrangements in microcirculation, oxyg... | 3,471 | [
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0.214062,
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0.1382867,
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Increased fibrinolytic activity in the endometrium of patients using copper-iud (gravigard). | The use of an IUD often causes an increase in the menstrual blood loss, and this is the most common reason for removing the device. The fibrinolytic activity of the endometrium was studied histochemically in 15 women before and after the use of copper-IUD for 2-5 as well as for 8-12 months. The fibrinolytic activity in... | 3,481 | [
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0.003657207,
... |
Urea transport-defective strains of Saccharomyces cerevisiae. | Experiments characterizing the urea active transport system in Saccharomyces cerevisiae indicate that (i) formamide and acetamide are strong competitive inhibitors of urea accumulation, (ii) uptake is maximal at pH 3.3 and is 80% inhibited at pH 6.0, and (iii) adenosine 5'-triphosphate generated by glycolysis in conjun... | 3,491 | [
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0.09198608,
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... |
The determination of phanquone in biological material by gas-liquid chromatography. | A gas-liquid chromatographic method for the quantitative determination of phanquone is described, based on the formation of a dimethoxine prior to its extraction from biological material. The sensitivity of the procedure is about 15 ng/ml in biological fluid. | 3,515 | [
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0.01872375,
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[Has the pH meter replaced the Apgar score?]. | The introduction of measuring the pH appears to place it in competition with Apgar scoring because of its precision. A study of this which has been carried out has illustrated that there are two different criteria for assessing the state of the infant at birth. The usual agreement between pH values and Apgar scoring ca... | 3,546 | [
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0.09734925,
0.4626196,
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Immunology of DNA. I. The influence of reaction conditions on the Farr assay as used for the detection of anti-ds DNA. | The sensitivity and the specificity of the Farr assay for the detection of antibodies to double stranded (ds) DNA depends very much on the reaction conditions. The interaction between ds DNA and anti-ds DNA is inhibited when ionic strength and pH are increased. ds DNA is bound by normal sera at ionic strength lower tha... | 3,611 | [
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Further evidence for histamine facilitating oestrogen action in the uterus. | The effect of doses of estradiol ranging from 0-0125 to 1-6 mug on the uterine weight of the spayed rat was studied 24 h after a single s.c. injection of the hormone. The lowest dose inducing a significant increase in uterine weight was 0-32 mug. When histamine dihydrochloride (50 mg) was simultaneously injected with t... | 3,616 | [
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-... |
Concentrations of bacteria in cerebrospinal fluid of patients with bacterial meningitis. | Concentrations of bacteria in cerebrospinal fluid ranged from 4.5 X 10(3) to 3 X 10(8) colony-forming units/ml in 27 patients with bacterial meningitis before antibiotic therapy and from 4 X 10(1) to 1.4 X 10(6) CFU/ml in four patients after one to two days of antibiotic therapy. All patients with persistent positive c... | 3,635 | [
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... |
Neurogenic influences of bilateral adrenalectomy on monoamine oxidase. | Bilateral adrenalectomy (10 days) increased the monoamine oxidase activity of the rat heart, vas deferens, spleen, superior cervical ganglion, and hypothalamus but not that of the rest of the brain, kidney, and liver. Experiments were made to determine whether the increased activity was due to neurogenic influences and... | 3,640 | [
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0.3176844,
0.0239634... |
Intraspecific variations in the hemolymph of Biomphalaria glabrata, a snail host of Schistosoma mansoni. | An attempt was made to characterize the hemolymph of Biomphalaria glabrata with reference to "normal" intra-specific variation, i.e., both inter- and intra-strain differences. Total protein concentration, per cent hemoglobin, pH, and osmolarity were studied. Seven geographic strains of B, glabrata were examined. In add... | 3,698 | [
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[Relevance of biochemistry in diagnosis and development of alcoholic liver disease (author's transl)]. | In a group of 205 patients with alcoholic diseases of liver the diagnostic relevance of biochemical tests (GOT, GPT, AP, GGTP, BSP) was reconsidered with discriminatory process (separation of diagnosis). The group contained 16 patients with nutritional-caused and 41 cases with alcoholic-caused fatty-infiltration of liv... | 3,720 | [
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0.184288,
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[A compact form of DNA in solution. 2. Peculiarities of acidic titration of double-stranded DNA in PEG-containing water-salt solutions]. | The formation of compact double-stranded DNA molecules in PEG-containing watersalt solutions (0.3 M NaCl) may be observed within the pH-range 3-10; i.e. under conditions at which parameters of double-stranded DNA helices are not strongly different from those of B-form. At pH less than 3, when regularity of double helic... | 3,728 | [
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Cyclic AMP and neuroendocrine influence upon forelimb regeneration in the adult newt, Notophthalmus viridescens. | The influence of the nervous and endocrine systems upon successive stages of limb regeneration suggest possible neuroendocrine interaction(s). Intraperitoneal injections of various agents had no effects upon blastema formation. Chlorpromazine, administered alone or with hormones, retarded morphogenesis. Prolactin, dibu... | 3,753 | [
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0.060760... |
On the influence of prostaglandin F2alpha-induced labor at term on the metabolism and coagulation of mother and fetus. | 11 pregnancies at term were terminated by dilatation of the uterine cervix, low amniotomy, and by intravenous administration of PGF2alpha. The average infusion time was 3 hours 55 minutes, and the average total dose of PGF2alpha amounted to 2.0 mg. Parameters of acid-base changes, carbohydrate and energic state changes... | 3,823 | [
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Dopamine receptor binding predicts clinical and pharmacological potencies of antischizophrenic drugs. | Tritiated haloperidol and tritiated dopamine label postsynaptic dopamine receptors in mammalian brain. Clinical potencies of butyrophenones, phenothiazines, and related drugs correlate closely with their ability to inhibit tritiated haloperidol binding. These binding methods provide a simple in vitro means for evaluati... | 3,854 | [
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0.302719,
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0.07325084,
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Metomidate as sole anaesthetic agent in tawny owls. | Metomidate 1 per cent was administered intramuscularly as the sole anaesthetic agent on 22 occasions to seven tawny owls (Strix aluco). Four deaths occurred, none on the first administration. ECG findings and details of dosage are provided. The possible causes of death are discussed. | 3,876 | [
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[Dynamics of production and various properties of interferon-like inhibitors formed under the influence of AET and cystaphos in vivo and in cell cultures]. | Interferon-like virus inhibitors appeared in the blood serum of animals and in the culture fluid 15 min.--1 hour and 4-6 hours after administration of AET (S, beta-amino-ethylisotiuronium) and cystaphos (monosodium salt of beta-aminoethylthiophosphorus acid). These inhibitors showed species-specificity, were inactivate... | 3,884 | [
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... |
[Lipolysis in human adipose tissue (author's transl)]. | Lipolytic activity was measured under different conditions in isolated fat cells and homogenates of human adipose tissue of the greater omentum. It was demonstrated that lipolysis took place in isolated fat cells at an optimum pH of 7.4 and was markedly stimulated by noradrenaline, but not by blood serum. By contrast l... | 3,890 | [
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... |
[Possibilities and limits of the intrauterine reanimation (author's transl)]. | Intrauterine reanimation means the removal of acute maternal or fetal distress. Beside of maternal shock (traumatic, hoaemorrhagic, supine hypotension syndrome) all acute distress situation are seen during labour. The therapeutical possibilities and premises on the one side, the limits and dangers on the other are disc... | 3,899 | [
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0.05114219,
0.4874652,
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Graft-versus-host reaction: a pathogenetic principle for the development of drug allergy, autoimmunity, and malignant lymphoma in non-chimeric individuals. Hypothesis. | It is proposed that the same diseases as those induced by the graft-versus-host reaction (GVHR) will arise in nonchimeric individuals, if structures of the major histocompatibility complex (MHC) on lymphocytes are altered, either by viral infection or by chemicals, in such a way that autologous T lymphocytes react agai... | 3,900 | [
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0.2293396,
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... |
[Preparation of the lectin B component from the hemagglutinin of Lens culinaris]. | Out of a mixture of both lectins of the lentil (Lens culinaris) the component B may be separated rapidly and easily because of the different temperature sensitivity of haptene protected isolectins A and B. | 3,927 | [
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-0.1321325,
-0.07... |
Bactericidal activity and pharmacology of flucloxacillin. | Flucloxacillin, a recent addition to the group of isoxazolyl penicillins, was studied in vitro and in normal volunteers. The bactericidal activity of the drug against most strains of gram-positive bacteria including penicillin-resistant Staphylococcus aureus was similar to that of oxacillin and approximately fourfold g... | 3,969 | [
-0.02218165,
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0.07741524,
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0.1906272,
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0.03725337,
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0.2431361,
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0.01732651,
-0.14969,
0.3843296,
0.184204,
0.3272643,
0.1011235,
... |
[Endocrine cells of the gastric mucous membrane]. | In order to obtain an additional information concerning a possible functional role of the main types of the endocrine cells (EC and ECL) on investigation of aspiration biopsy specimens taken from the middle third of the stomach in patients with ulcerative disease of the duodenum and chronic gastritis was carried out. C... | 4,052 | [
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0.1396493,
0.2614622,
0.3335192,
0.5471235,
-0.08434604,
-0.07997... |
Anomalous fluorescence of yeast 3-phosphoglucerate kinase. | The 3-phosphoglycerate kinase (EC 2.7.2.3) of yeast which contains two tryptophyl and eight tyrosyl residues per molecule, displayed an unusualy fluorescence emission spectrum with a maximum at 308 nm when excited at 280 nm. The emission peak shifted to 329 nm when excited at 295 nm. We could confirm that it was due to... | 4,108 | [
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0.1900144,
0.009289912,
0.01883676,
0.5341886,
0.3348867,
0.05602068,
0... |
A study of the single polypeptide nature of rhodanese. A comparison of different preparations. | The enzyme rhodanese (EC 2.8.1.1) appears as a single polypeptide chain protein on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The molecular weight of this species is approx. 33 000. This contrasts with previous reports that rhodanese behaves on gel filtration chromatography as a rapidly equilibrating mo... | 4,117 | [
-0.1899378,
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0.004040258,
0.06919662,
0.2831079,
0.1913854,
0.1043731,
-0.0900456... |
Purification and properties of NADP-dependent glutamate dehydrogenase from yeast nuclear fractions. | 1. NADP-dependent glutamate dehydrogenase (EC 1.4.1.4) extracted from nuclear fractions of Saccharomyces cerevisiae was partially purified. The final purification achieved was over 100-fold over the initial extract. 2. Cellulose acetate electrophoresis shows that the preparation is close to homogeneity and that the enz... | 4,126 | [
-0.06631706,
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0.3346549,
0.4796706,
0.376965,
0.1756221,
0.0... |
Ascorbic acid-2-sulfate sulfhohydrolase activity of human arylsulfatase A. | Pure human arylsulfatase A (EC 3.1.6.1) was found to hydrolyze ascorbic acid 2-sulfate to ascorbic acid and inorganic sulfate at rates from 200 to 2000 mumol/mg per h depending on the method of assay. This rate was lower than that observed with the synthetic substrate 4-nitrocatechol sulfate, but higher than that seen ... | 4,134 | [
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0.1909733,
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0.3885888,
0.3174337,
0.2365147,
-0.1055494,
-0.0... |
Evidence against proton gradient formation being the cause of chlorophyll fluorescence quenching by N-methylphenazonium methosulfate. | In strong illumination, 3-(3, 4-dichlorophenyl)-1,1-dimethylurea (DCMU)-poisoned chloroplasts exhibit a high yield of chlorophyll fluorescence while P-700 turnover, proton uptake, and phosphorylation are inhibited and a pH gradient is undectectable. When 10muM N-methylphenazonium methosulfate (PMS) is included, the flu... | 4,143 | [
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0.3067891,
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0.1977538,
0.4524459,
0.2570966,
0.1175158,
-0... |
The accumulation of superoxide radical during the aerobic action of xanthine oxidase. A requiem for H2O4. | The action of xanthine oxidase upon acetaldehyde or xanthine at pH 10.2 has been shown to be accompanied by substantial accumulation of O2- during the first few minutes of the reaction. H2O2 decreases this accumulation of O2- presumably because of the Haber-Weiss reaction (H2O2+O2- leads to OH- +OH+O2) and very small a... | 4,144 | [
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0.096803,
0.0171627,
0.34036,
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0.163222,
0.3... |
Purification and properties of cyclic AMP dependent and independent protein kinases from rat pancreas. | Three protein kinases Ko, K1, and KII have been extracted from rat pancreas homogenate, Ko is not stimulated by cyclic AMP. K1 is poorly stimulated by cyclic AMP (1.3 times), Ku is highly stimulated (6 times). The specificity of KII with respect to various nucleotides and cyclic nucleotides has been determined. K1 and ... | 4,157 | [
-0.05916566,
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0.02022614,
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0.1250051,
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0.07365164,
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0.007901178,
0.270927,
0.09734397,
-0.012229... |
[Interactions of phosphorylethanolamine analogs with phosphorylethanolamine-citidylyltransferase]. | Kinetic studies of ethanolaminephosphate-cytidylyltransferase (E.C. 2.7.7.14) from rat liver have been carried out in presence of structural analogues of ethanolaminephosphate : these compounds acted as inhibitors of the enzyme: - 2-aminoethylphosphonate behaved as a substrate and a competitive inhibitor to phosphoryle... | 4,159 | [
-0.240525,
0.247924,
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0.1453335,
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0.6022126,
0.3444618,
0.08097126,
-0.06419054,
0.01... |
Superficial cell-wall layers on Spirillum "Ordal" and their in vitro reassembly. | The cell envelope of Sporillum sp. strain "Ordal" (possibly a variety of S. anulus) demonstrated multiple superficial wall layers which were diverse in their macromolecular arrays. Negative staining and freeze-etching techniques revealed an outer hexagonally packed layer and an inner tetragonally packed layer. However,... | 4,216 | [
-0.3754691,
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0.1517522,
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0.1746831,
0.6175513,
-0.08344927,... |
Premature labour. | Prematurity is by far the commonest cause of neonatal morbidity and mortality. The management of premature labour is empirical because little is understood about the mechanism of labour. Effective uterine relaxant drugs have an important, albeit minor role. Phototherapy has reduced the complications of neonatal hyperbi... | 4,217 | [
0.2019489,
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0.1052619,
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0.01528922,
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0.2441097,
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-0.252773,
0.3281825,
0.1052527,
-0.1331061,
-... |
31P nuclear magnetic resonance-pH titrations of myo-inositol hexaphosphate. | With the use of 31P n.m.r. spectroscopy, the separate pKa values of each of the six phosphoric monoester groups of myo-inositol hexaphosphate were determined. The range of hydrogen-ion concentrations covered extended from that required for the phosphonium salts to that for the full dodecyl anion, and the determinations... | 4,222 | [
-0.3810947,
0.006473595,
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0.04404868,
0.1870249,
0.390909,
0.001341095,
0.1381542,
0... |
Purification and properties of human acid-thermostable ribonucleases, and diagnosis of childhood pancreatic fibrosis. | Acid-thermostable ribonucleases were isolated from human pancreas, duodenal contents, liver, spleen, serum and urine, and purified 15--1000-fold. The pH optima, ionic requirements, and some of the specificity requirements, of these enzymes were investigated. The isolated enzymes formed two distinct groups: (a) The ribo... | 4,243 | [
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0.1441398,
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0.1754392,
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0.2464931,
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0.2743943,
0.1418511,
0.15376,
-0.2907672,
0.39... |
Physico-chemical and immunological properties of acid alpha-glucosidase from various human tissues in relation to glycogenosis type II (Pompe's disease). | The physico-chemical and immunological properties of acid alpha-glucosidase from various human tissues have been studied. Heat stability of acid alpha-glucosidase from heart, liver and skeletal muscle is identical, but for kidney some different results are obtained. Identical isoelectrofocussing patterns are found for ... | 4,245 | [
-0.06021284,
-0.001913569,
-0.4192528,
0.140264,
0.2188861,
0.02297962,
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0.006815821,
0.4000742,
0.09696176,
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0.1632624,
0.3165913,
0.1970832,
0.5034102,
0.421218,
-0.3369682,
-0... |
Assessment of the therapeutic effect of epimestrol and epimestrol associated with clomiphene in female sterility. | In 24 women with disturbances of ovulation treated for sterility with Epimestrol, ovulation was achieved in 3 patients but none of these became pregnant after therapy. Since it has been suggested that the association of Clomiphene with a weak estrogen might improve the pregnancy rate, we decided to administer Clomiphen... | 4,384 | [
-0.05606789,
0.4159977,
0.06767535,
-0.06259935,
-0.02130022,
-0.1351521,
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0.03149024,
-0.02843599,
0.1380669,
0.06566067,
-0.00006439075,
-... |
Improvement of fertility and semen quality in men treated with a combination of anticongestive and antibiotic drugs. | The effects of a combined therapy of antibiotics and antiinflammatory drugs was evaluated in 344 men referred to our clinic for treatment of infertility. Physical signs of congestion, usually not severe, were detected in 244 men. The treatment caused significant improvement in semen quality, especially in sperm concent... | 4,385 | [
0.2777577,
0.4773155,
0.06238405,
0.03003832,
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0.1747453,
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0.009865982,
-0.08825145,
0.08742023,
-0.01829491,
0.05217835,
-0.255169... |
Hypogastric carotid bypass for Takayasu's disease. | Two cases of Takayasu's disease are presented which were successfully resolved by a Dacron graft from the right hypogastric artery to the right internal carotid artery. We recommend this bypass procedure of connecting the hypogastric artery to one of the aortic branches. Medical treatment with antibiotics, steroids and... | 4,405 | [
-0.06156954,
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0.01362137,
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-0.3766477,
0.2296034,
-0.1440472,
-0.05397238,
0.1140586,
0.04973581,
0.020... |
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