text stringlengths 10 379k |
|---|
Total tobacco cessation is the only treatment that improves the symptoms and reduces the risk of amputation in Buerger's disease. Other forms of treatment are not well established or have unfavorable results. Surgical revascularization is often ineffective because the distal target vessels are often involved in this di... |
A 19-year-old female presented with recurrent hemoptysis and oral ulceration. Her first experience of hemoptysis had been 2 years earlier; she was admitted to a local hospital at that time. Bronchofiberscopy revealed diffuse alveolar hemorrhage; however, there were no specific findings for the cause of hemoptysis. The ... |
A five-month-old male infant (weight, 6 kg; height, 64 cm) underwent elective repair of a large perimembranous-type ventricular septal defect (VSD). He had no discernible anomaly other than VSD; his preoperative routine laboratory values were all within normal limits, and he had no family history of genetic disease inc... |
An 86-year-old female was referred to Seoul St. Mary's Hospital because of abnormal chest X-ray findings. The chief complaints were intermittent cough and mild fever ongoing for one month. Computed tomography (CT) revealed a mass (3.3×2.2 cm) in the lingula of the left upper lobe (LUL) and a second lesion (2.2×1.6 cm) ... |
Myelolipoma is a rare, benign tumor composed of hematopoietic tissue and mature adipose tissue. It is nonfunctioning and often detected in the adrenal glands during routine X-ray or computed tomography (CT) as incidentalomas. Extramedullary myelolipomas are extremely rare, and half of them are located in the presacral ... |
A 60-year-old male was referred to the Asan Medical Center for a nodule in the left lower lobe (LLL). The nodule was incidentally detected during the preoperative evaluation of gallstone, for which he underwent laparoscopic cholecystectomy at an outside hospital three months prior to his presentation to us. On his visi... |
Mediastinal paragangliomas are very rare neuroendocrine tumors. Most of the tumors are benign, but some of them can be diagnosed as malignant []. Complete resection is the standard treatment of paragangliomas []. However, the highly vascular nature of these tumors and their characteristic anatomic locations make comple... |
Cancer morbidity worldwide is growing. However in the United States, the overall morbidity slightly decreased in 2013, (by >0.6%/yr in men, but it was stable in women), while the mortality rates decreased by 1.8%/yr in men and by 1.5%/yr in women []. The lung was the site of the highest cancer mortality in both the sex... |
Partial anomalous pulmonary venous connection (PAPVC) is an anomaly in which some but not all pulmonary veins connect to the right atrium or its tributaries as opposed to the left atrium []. The most common type of PAPVC is related to a sinus venosus malformation; the right upper and middle pulmonary veins connect to t... |
Oral diseases are one of the most prevalent conditions in the world and are largely preventable.
Dental caries affects 60-90% of school children and most adults in industrialized countries; it is increasingly prevalent in developing countries and highly prevalent in some Asian and Latin American countries.[] Periodonta... |
Injuries of the maxillofacial complex represent one of the most important health problems worldwide.[] Maxillofacial injuries, such as soft-tissue injuries, dental injuries, or maxillary, mandibular, and zygomatic fractures; are the most common injuries treated by oral and maxillofacial surgeons.[]
The mandible is a un... |
U
n
d
e
r
s
t
a
n
d
i
n
g
c
a
r
i
e
s
e
t
i
o
l
o
g
y
a
n
d
i
t
s
p
r
o
g
r
e
s
s
i
o
n
h
a
s
b
e
e
n
m
a
d
e
e
a
s
y
w
i
t
h
c
o
n
c
o
m
i
t
a
n
t
a
d
v
a
n
c
e
s
i
n
s
c
i
e
n
c
e
a
n
d
t
e
c
h
n
o
l
o
g
y
.
H
o
w
e
v
e
r
,
u
t
i
l
i
z
a
t
i
o
n
o
f
t
h
o
s
e
a
d
v
a
n
c
e
s
... |
Hepatitis B infection (HBI) is one of the major public health problems globally and is the 10 leading cause of death. Worldwide, more than two billion of the population have evidence of past or recent HBV infection and there are more than 350 million chronic carriers of this infection.[] The number of HBsAg carriers in... |
Hospital waste is often described as any residual matter, solid or liquid that is generated in the diagnosis, treatment or immunization of human beings or animals.[] According to World Health Organization estimates 85% of hospital waste is actually non-hazardous and around 10% is infectious while the remaining 5% is no... |
xref
sup
italic
#text
xref
sup
#text
presents the general profile of the study population. The age group of 35-44 years contributed for more than one-third of the sample size, whereas there were fewer subjects who belonged to the youngest age group (18-24 years). The overall prevalence of periodontal disease was 98.9%.... |
The society has collective consciousness. The beliefs, practices and attitudes can be studied in a same way as can be an individual mind with appropriate methods. Absence of scientifically supported background knowledge of a society can severely hinder any attempt of change, whether good or bad in a society. This makes... |
Dental caries has been described as a disease involving the localized destruction of the tooth tissue by microorganisms. It is a chronic disease process that usually progresses slowly and infrequently is self-limiting. Dental caries can affect enamel, dentine, and cementum. It manifests clinically along a continuum fro... |
Agenesis of one or more teeth in primary or permanent dentition is known as hypodontia, while, hyperdontia is a condition that of having extra to the normal complement of teeth. Hypodontia and hyperdontia are two extremes in the development of the dentitions. The occurrence of both these identities in the same individu... |
Significance of any disease in particular area can be gazed by its prevalence. This becomes even more important for developing country like India where oral health program and preventive measures are far from satisfying needs.
Prevalence of malocclusion has been studied in adolescents of Cicero,[] Negro children of Col... |
The soft–shell turtle was purchased from a local farmer in Japan, and the green sea turtle was provided by the Genome 10K Project (originally collected in Ocean Park, Hong Kong). Genomic DNA was extracted from the whole blood of a female individual in each species, and we constructed a total of 18 (for the soft–shell t... |
To investigate the effect of combined treatment of staurosporine and ATRA on NB4-R1 and NB4-R2 cells, we first tested the concentration of staurosporine studied in both cell lines. DMSO treatment was regarded as control since both ATRA and staurosporine were dissolved in it. 2 nM staurosporine was determined to be used... |
As shown in , we proposed a composite structure including an open-end waveguide and a planar-spiral phase plate (planar-SPP) to generate OAM radio waves, which functions like a transmit array antenna and transforms an incoming phase front into a desired outputting phase front. The open-end waveguide is adopted to provi... |
Soil inorganic N content was higher in 2007 than 2008 ( = 28.9, < 0.001; ). Both N ( = 202.2, < 0.001) and water addition ( = 59.0, < 0.001) significantly increased soil inorganic N content (). Across all treatments, ANPP was higher in 2007 than 2008 ( = 9.9, < 0.01; ). Across the two years, water addition signific... |
Structure of the magnetic-assisted TENG is shown in , which includes the top steel mass with the weight of 18.0 g, top NdFeB permanent magnet with the radius of 9.5 mm and the height of 1.5 mm, silica layer, spiral-shaped electrode wrapped by polyimide, and the bottom NdFeB permanent magnet with the radius of 5.0 mm an... |
The selected maize inbred lines used in this study were recombinant inbred lines developed from F plants of the cross Mp715 × Va35. There were two identical copies for each gene in each maize inbred line. Only up to two different alleles for each gene were present among all the six maize inbred lines because they were ... |
To investigate phage resistance mechanism in , wild-type strain PA1 was infected with phage PaP1 (MOI = 1000) and screened for phage-resistant mutants, which could be consistently recovered in high frequency (3.02 ± 0.85 *10) (). Interestingly, a high percentage (31% ± 13.2%) of the mutants produced red pigment, and tw... |
Using time-lapsed imaging, we recorded the cell growth and examined the structure and cell division dynamics for both muscle stem cells from mouse and rat, and human mesenchymal stem cells. Time-lapsed images were acquired at 10-minute intervals over the course of 5 days. From these images, we obtained direct measureme... |
The ternary composites were prepared with a two-step protocol as shown in . After a first step of coating solution-exfoliated GO nanosheets with polyaniline, MnO nanorods were allowed to self-assembly onto the GO sheets and then wrapped by in-situ prepared polyaniline coatings in the second step. The MnO (in weight rat... |
Although most complex dynamic systems are nonlinear, the controllability of nonlinear systems is in many aspects structurally similar to that of linear systems. Actually, to ultimately develop the control strategies for complex nonlinear networks, a necessary and fundamental step is to investigate the controllability (... |
In recent works, integrated quantum circuits established on silicon waveguides are adequately investigated. Utilizing the femtosecond laser waveguide writing technology, on-chip devices to support and manuscript polarization-encoded qubits have been fabricated. A controlled-NOT gate has been experimental demonstrated w... |
To characterize the role of MreB in the GVE2 infection, the MreB gene was cloned from . E263. The results showed that the E263 strain contained MreB gene (), the sequence of which was highly conserved with that of mesophilic bacteria (data not shown), suggesting that MreB in thermophiles shared similar/same functions a... |
xref
#text
T
h
e
r
e
a
r
e
(
a
t
l
e
a
s
t
)
t
h
r
e
e
c
o
m
m
o
n
o
b
j
e
c
t
i
o
n
s
t
o
t
h
e
v
i
e
w
t
h
a
t
n
o
n
-
m
y
o
c
y
t
e
s
m
a
y
b
e
e
l
e
c
t
r
i
c
a
l
l
y
c
o
u
p
l
e
d
t
o
m
u
s
c
l
e
c
e
l
l
s
i
n
n
a
t
i
v
e
c
a
r
d
i
a
c
t
i
s
s
u
e
.
Gap junctions, in par... |
It is established that kidney function is reduced with age, but since there is a co-variation with vascular disease, the precise ageing effect is uncertain. There are studies strongly supporting the hypothesis that a decline in kidney function is related to normal senescence [, ]. In the indigenous people of Kuna, who ... |
The rising prevalence of chronic disease and disability with older age increases the need for healthcare services in the ageing population. Healthy living habits play a major role in the ageing process [, ], but other exposures that the persons face during the life course increase the risk of adverse health outcomes in... |
Improving mental wellbeing throughout the life course is recognised as an important component of healthy ageing []. Healthy ageing is influenced by an individual's socioeconomic position throughout the life course [, ], but also by societal level factors such as the welfare state [, ]. Early old age, containing both th... |
demonstrates the fabrication process of hybrid (ultra)thin films supported on porous anodic alumina (PAA) membranes. It should be noted that all of the thin films presented in this work are hybrid films using poly(3,4-ethylenedioxythiophene) doped with poly(styrenesulfonate) (PEDOT:PSS) as matrices. PEDOT:PSS is a conj... |
Epilepsy surgery is the most effective treatment option for pharmacoresistant epilepsy, but is often hindered by difficulty in accurately delineating the epileptogenic zone. Although there have been great advances in surgical localization from the use of magnetic resonance imaging (MRI) during the previous decades, MRI... |
T
e
m
p
o
r
a
l
l
o
b
e
e
p
i
l
e
p
s
y
(
T
L
E
)
,
e
s
p
e
c
i
a
l
l
y
m
e
s
i
a
l
t
e
m
p
o
r
a
l
l
o
b
e
e
p
i
l
e
p
s
y
(
m
T
L
E
)
i
s
t
h
e
m
o
s
t
c
o
m
m
o
n
f
o
r
m
o
f
f
o
c
a
l
e
p
i
l
e
p
s
y
i
n
h
u
m
a
n
s
(
T
a
n
d
o
n
1
9
8
9
)
.
T
h
e
p
a
t
h
o
l
o
g
i
c
a
... |
Status epilepticus (SE) has traditionally been defined as more than 30 minutes of continuous seizure activity or two or more sequential seizures without full recovery of consciousness in between for a total of more than 30 minutes.[] In 1999, Lowenstein . proposed a new working definition that lowered the threshold to ... |
There has been a progressive increase in life expectancy in the general population over the last several decades. This trend is likely to continue, and it has been suggested that the first person to live up to 150 years may have already been born! Epidemiological studies indicate that the incidence and prevalence of ep... |
xref
#text
T
h
e
a
n
t
e
r
i
o
r
t
h
a
l
a
m
i
c
n
u
c
l
e
u
s
i
s
t
h
e
m
o
s
t
w
e
l
l
-
s
t
u
d
i
e
d
t
a
r
g
e
t
f
o
r
s
t
i
m
u
l
a
t
i
o
n
i
n
e
p
i
l
e
p
s
y
w
i
t
h
o
t
h
e
r
r
e
p
o
r
t
e
d
t
h
a
l
a
m
i
c
t
a
r
g
e
t
s
b
e
i
n
g
c
e
n
t
r
o
m
e
d
i
a
n
(
C
M
)
n
u
c
l... |
Magnetoencephalography (MEG) measures the magnetic fields generated by electric currents in the brain. The magnetic field measurements are in the range of femto-tesla to pico-tesla. MEG provides a very accurate resolution of the timing of neuronal activity.[] This is a non-invasive test.
MEG is a direct measure of brai... |
Surgical decision-making is a complex process. The parties involved are usually the surgeon, the patient, the families, society, insurance companies and the hospital administration. What are the possible outcomes of such situations when multiple parties are interacting in self-interest? What are the “optimal” outcomes?... |
xref
#text
It is estimated that there are more than 10 million persons with epilepsy (PWE) in India. Its prevalence is about 1% in our population.[] The prevalence is higher in the rural (1.9%) compared to urban population (0.6%)[] In the Bangalore Urban Rural Neuro-epidemiological Survey (BURNS), a prevalence rate of ... |
Drug-resistant epilepsy (DRE) is defined as failure of adequate trials of two (or more) tolerated, appropriately chosen and appropriately used antiepileptic drug regimens (whether administered as monotherapies or in combination) to achieve freedom from seizures.[] Establishing a diagnosis of DRE is an important milesto... |
xref
#text
xref
#text
In cases of lesional epilepsy, it is generally believed that seizures arise from the vicinity of the lesion. However, the precise location is not certain and cannot be determined on the basis of imaging abnormalities. Over the years, many have tried to use EEG recording acutely during surgical int... |
xref
#text
xref
#text
The underlying cause of SE determines its outcome to a great extent.[] When possible the etiology of SE should be treated promptly. The etiology of SE depends on the geographical region; in the Veterans Administration co-operative study, the etiology of SE was remote neurologic cause in 69.5% and ... |
Epilepsy that cannot be controlled with known pharmacological management, also referred as drug-resistant epilepsy (DRE), patients are recommended for epilepsy surgery.[] The genesis of DRE still eludes us, but abnormal synaptic transmission is one of the key features of this disorder. Epilepsy can be caused by multipl... |
Epilepsy is one of the most common serious neurological conditions, accounting for 1% of the global burden of disease, based on disability-adjusted life years, the number of person years lost due to disability and premature death.[] This is equivalent to breast cancer in women and lung cancer in men. Among primary diso... |
T
h
e
e
p
i
l
e
p
s
y
c
o
m
p
r
i
s
e
s
a
h
e
t
e
r
o
g
e
n
e
o
u
s
g
r
o
u
p
o
f
d
i
s
o
r
d
e
r
s
c
l
i
n
i
c
a
l
l
y
c
h
a
r
a
c
t
e
r
i
z
e
d
b
y
r
e
c
u
r
r
e
n
t
u
n
p
r
o
v
o
k
e
d
s
e
i
z
u
r
e
s
a
n
d
e
l
e
c
t
r
i
c
a
l
l
y
c
h
a
r
a
c
t
e
r
i
z
e
d
b
y
a
s
u
d
d
e
n
s
... |
xref
#text
In general, the term HFOs refers to EEG activity >70 Hz, whereas activity in the 30-70 Hz band is considered as gamma.[] Our ability to record and analyze high frequency activity can be influenced by various factors. A thorough knowledge of such factors is important to navigate and interpret the expanding li... |
India is the second largest populous country in the world with a current population of 1.2 billion. With a prevalence rate of five per 1,000 person-years and an incidence rate of 50 per 100,000 person-years, it is estimated that at any given time, India has at least 5 million people with active epilepsy, to which nearl... |
xref
#text
Several common questions are asked in deciding where and how to use intracranial electrodes []. The most common question, which is raised when there is a lesion (acquired, neoplastic or developmental) is, “Where do the seizures begin in relation to the lesion?” Answering the question will confirm that the le... |
xref
#text
xref
#text
xref
#text
Lesionectomy for epilepsy is a surgical procedure that is directed at the structural lesion, believed to be the etiology of the seizure disorder.[] In the case of isolated structural lesions, such as dysembryoplastic neuro-epithelial tumors, low-grade astrocytomas, or focal vascular abn... |
E
x
t
r
a
t
e
m
p
o
r
a
l
l
o
b
e
e
p
i
l
e
p
s
i
e
s
(
E
T
L
E
)
a
r
e
c
h
a
r
a
c
t
e
r
i
z
e
d
b
y
t
h
e
e
p
i
l
e
p
t
o
g
e
n
i
c
f
o
c
i
o
u
t
s
i
d
e
t
h
e
t
e
m
p
o
r
a
l
l
o
b
e
.
T
h
e
y
h
a
v
e
a
w
i
d
e
s
p
e
c
t
r
u
m
o
f
s
e
m
i
o
l
o
g
i
c
a
l
p
r
e
s
e
n
t
a
t
i
... |
xref
#text
The search strategy spanning from a period of 1970s to 2012 was conducted using MEDLINE via PUBMED and the Cochrane databases using following search terms: ‘vertical root fracture’, ‘diagnosis of vertical root fracture’, ‘management of vertical root fracture’. A hand search was also carried out of the last t... |
Pulpstones are a group of calcified masses in the dental pulp of healthy, diseased and unerupted teeth.[] Stones may exist either freely within the pulp tissue or be attached to or embedded in dentin.[] A single tooth may have 1-12 pulp stones or even more. The size may vary from a small microscopic particle to large m... |
The restoration of the endodontically treated tooth is an important aspect of successful endodontic therapy. There are wide ranges of treatment options of varying complexity. The clinician must be able to predict the probability of restoring such teeth successfully. In general, endodontically treated teeth experience s... |
xref
italic
#text
This study was conducted in the Department of Conservative Dentistry and Endodontics. Teeth sterilization (gamma irradiation at 25 kGy)[] was performed at Microtol, Bangalore. Data was obtained using an inverted confocal laser scanning microscope (CLSM) (ZEISS LSM 510 META. GmbH, Mannheim, Germany) a... |
The origin of the fluorescence is not fully clear,[] though it seems unlikely that apatite is responsible for the basal values associated with healthy enamel.[] The explanation may be the result of the combination of the inorganic matrix with the absorption of organic molecules.[] Carious lesions fluoresce more strongl... |
During the root canal preparation procedures, dentin chips, pulp tissue, microorganisms and/or irrigants may get extruded into the periradicular tissues. Though a thorough control of the working length (WL) may decrease the risk, but nevertheless extrusion of any debris may potentially cause post-operative complication... |
Root canal microflora is the foremost cause of periradicular pathosis. A complex root canal system anatomy makes elimination of micro-organisms difficult and irrigants are essential since canal instrumentation alone is unable to remove all tissue remnants and debris. The desirable properties of an irrigant thus include... |
Root canal shaping is one of the most important steps in canal treatment.[] It is essential in determining the efficacy of all subsequent procedures, including chemical disinfection and root canal obturation.[] This “ideal” preparation can be a difficult task to achieve in severely curved root canals or S-shaped canals... |
Pre-flaring is recommended for elimination of cervical interferences. One problem due to use of the instruments for pre-flaring is whether they could create an increased risk for perforations, especially in mesial canals of mandibular molars.[] One of the iatrogenic factors in different methods of root canal preparatio... |
White spot lesions are early signs of demineralization under intact enamel, which may or may not lead to the development of caries. White spot lesions occur when the pathogenic bacteria have breached the enamel layer and organic acids produced by the bacteria have leached out a certain amount of calcium and phosphate i... |
Improvements in the mechanical properties of composite resins and esthetic needs have brought about an increase in the application of these materials. Weak bonding to dentin surface remains, however, is deemed as one of the major problems of composite resin restorations especially in gingival floor of cavities.[] Appli... |
The success of endodontic treatment is mainly dependent on thorough cleaning, shaping and disinfection of root canal system. The irrigation protocol thereby plays a key role in the disinfection of the root canal space.[] A clean root canal system along with a three dimensional seal is the clinician's road to success. S... |
The success of endodontic treatment is directly influenced by proper debridement of root canal including smear layer.[] Smear layer acts as a barrier and prevents bacterial invasion of the dentinal tubules.[] However, bacteria might survive and multiply in the smear layer and can also penetrate into dentinal tubules. I... |
A periapical lesion is a lesion involving the apical area of the tooth. The initial response of the dental pulp to injury is not significantly different from that seen in other tissues. However, the final result can be dramatically different because of rigid dentinal walls of the pulp chamber, which leads to the format... |
The principal aim of dental operator is to carry out the dental procedure with as little pain and discomfort as possible. One of the most distressing aspects of dentistry for the average dental patient is the fear and anxiety caused by the dental environment, particularly the dental injection, i.e., syringe and needle ... |
To ensure a successful restorative procedure, the bonded interface area must be capable of withstanding the stresses caused by polymerization shrinkage, occlusal loading and thermal changes. Marginal discoloration, recurrent caries and post-operative sensitivity may be associated with the penetration of bacteria and or... |
Successful endodontic therapy involves instrumentation of the root canal to produce a surface free from debris and organic matter and obturation to achieve an optimally-sealed canal.[] Gutta-percha has been universally accepted as the gold standard for root filling materials and the material against which others are co... |
Restoration of endodontically treated teeth may pose a restorative challenge because of damage by caries and endodontic procedures.[] To provide sufficient retention to the core, a radicular support is provided in the form of a dowel.[] Some authors believed that placement of dowel may reinforce the tooth,[] but unnece... |
Bernard-Soulier syndrome (BSS) was first recognized by two French hematologists, Jean Bernard and Jean Pierre Soulier in the year 1948 in a young male patient who has prolonged bleeding time, mild to moderate thrombocytopenia and very large platelets.[] It is known by other names such as hemorrhagiparous thrombocytic d... |
xref
#text
A 38-year-old healthy Asian woman was referred to the endodontic clinic by a general dentist. The patient presented to her dentist with a severe pain in left mandibular region ten days ago. The dentist initiated the root canal treatment in left mandibular canine (tooth # 22). During the cleaning and shaping ... |
We previously demonstrated that an inflammatory environment inhibits BMP-2-induced bone mass and osteoblastic differentiation of BMSCs through inflammatory cytokines, including TNF- and IL-1. However, the mechanism responsible for this phenomenon is complicated and unclear. Therefore, we examined the effect of TNF-/I... |
As a mitochondrial chaperone in the mitochondrial matrix, mortalin is critically required for the proper import and folding of nuclear-encoded matrix proteins. We hypothesized that PD-associated loss of mortalin function initiates impaired mitochondrial protein homeostasis. We first sought to measure the ratio of nucle... |
Previous studies have shown that ER stress causes cell death through accumulation of unfolded or abnormal proteins in the ER and subsequent activation of ER stress-induced caspases. ER stress transducers modulate ER-specific stress; therefore, we investigated whether the main ER stress transducer IRE1 regulates ER stre... |
Preliminary screening by 3-[4,5-dimethyl-thiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay was performed to ascertain the time and dose of the combined treatment of SeC with AF on several human cancer cells and normal cell. The results showed that treatment of human cancer cells with SeC for 48 h or AF for 6 h ... |
To test whether APN affects hearing impairment, we assessed auditory brainstem response (ABR) thresholds in WT and APN-KO mice at the ages of 2 months and 6 months (=5–7 per group). WT mice did not show any threshold shifts at any frequencies at these ages. At 2 months of age, APN-KO mice exhibited a significant increa... |
First, we examined the presence of N-terminal fragment of profilaggrin in nuclear fractions of cultured keratinocytes. Profilaggrin itself was detected at day 5 and strongly expressed at day 7 of prolonged culture after confluency (). Anti-A+B domain antibody (H-300) detected a fragment of 55 kDa in the nuclear extract... |
To address the role of autophagy in VSMC foam cell formation, first, we detected the changes of autophagy in oxLDL-treated VSMCs. As shown in , exposure to oxLDL decreased the LC3II/LC3I ratio and beclin-1 level in a time-dependent manner in VSMCs, with an obvious effect at 24 h post oxLDL challenge. Moreover, a marked... |
It has been suggested that soluble factors secreted from MSCs have an important role in inhibiting antibody-producing B cells. Thus, we attempted to evaluate the regulatory activity of conditioned medium (CM) of MSCs (MSC-CM) harvested from our clonal MSCs on the Ig production by B cells. When splenic B cells isolated ... |
Aberrant expression of miR-185 has been reported in several types of cancers. To explore the potential role of miR-185 in human gastric cancer, we analyzed 25 pairs of human gastric cancer tissues and matched adjacent noncancerous tissues (). Quantitive analysis revealed that miR-185 levels were significantly reduced i... |
The cytotoxic effect of 5-FU in the SNUC5 cell line and its 5-FU-resistant variant, SNUC5/5-FUR, was compared. The concentration of 5-FU that yielded 50% growth inhibition (IC) was 9 M in SNUC5 cells and 2264 M in SNUC5/5-FUR cells, in which resistance was induced by continuous culture in 140 M 5-FU (). These results w... |
This study hypothesized that CuE could mediate the survival of primary CRC cell lines, thereby inhibiting proliferation. To explore the antitumor activity of CuE against CRC cells, we initiated an study in which each of the CRC cell lines was exposed to increasing doses of CuE (0, 2.5, 5, and 7.5 M) over a period of 2... |
For analyzing the ischemic responses of 14-3-3 proteins in neurons, isoform-specific 14-3-3 antibodies were used. Results of western blot demonstrated that 14-3-3, , , and antibodies recognized the corresponding overexpressed 14-3-3 isoform only (). Then, we measured the expression of 14-3-3 isoforms in primary cultu... |
The levels of XAF1 expression were determined in both the renal tissues of Thy-1N rats () and cultured rat GMCs after sublytic C5b-9 stimulation (). The time course study showed that the expression of XAF1 protein both and increased at 2 h, peaked at 6 h and then reduced at 12 h (). Thus, we examined the expression o... |
An NIH3T3 mouse fibroblast cell line expressing GFP-tagged histone H3 was generated to facilitate the imaging of mitosis in living cells. The NIH3T3/H2B-GFP cells were transfected with an siRNA library targeting the mouse kinome complement (also included some phosphotransferases and protein kinase regulators). Each of ... |
To assess the role of Met in cardiac protection from hypoxia injury, we studied the H9c2 rat cardiomyoblasts. These cells express cardiac markers Connexin43 and Troponin-I (). First, we demonstrated using flow cytometry that the endogenous Met receptor is present at the cell surface (). Moreover, immunofluorescence (IF... |
Akt phosphorylation is widely established to be one of the key-stone events involved in the regulation of prostate cancer cell survival. Since ML-9 has been suggested to be the potent inhibitor of Akt phosphorylation in other cell models, we first checked for this function of ML-9 in prostate cancer cells. In lymph nod... |
In a systems biological approach, we have previously generated gene expression profiles from 48 normal and 47 tumor prostate tumor tissue samples. Here, gene expression analyses of BRD4 revealed a significant increase of in our prostate tumor samples (). To investigate which BRD4 target genes are most significantly de... |
We previously showed that HSF1 controls the stability of mutp53 protein in human cancer cells via activation of Hsp90, which strongly stabilizes mutp53 (Li and ). Conversely, here we show that mutp53 also induces HSF1 in all human cancer cell lines and mouse primary cells tested. First, RNAi-mediated depletion of mutp... |
We used a wound healing assay to measure the migration rate of 4T1 cells with and without GC treatment (100 g/ml of GC for 24 h). GC significantly reduced the motility of 4T1 cells compared with the untreated control for up to 6 h (). Cell migration rates were quantified using ImageJ software (National Institutes of He... |
We previously identified PDGFR cells in the interstitial space of mouse skeletal muscle and showed that these cells represent a unique mesenchymal progenitor population distinct from satellite cells. Because PDGFR mesenchymal progenitors have been demonstrated to contribute to pathologic changes of skeletal muscle such... |
Features of NL (normal liver) individuals and NAFLD patients studied are detailed in . All groups were well matched in terms of age and sex distribution. In the NAFLD cohort, NAS patients had significantly higher body mass index (BMI) mean (=0.017), insulin (=0.007), homeostasis model assessment of insulin resistance (... |
We commenced this study by evaluating the subcellular expression and radioinducibility of APPL1 and APPL2 in the pancreatic carcinoma cell lines MiaPaCa2 and Capan-1. Both APPL1 and APPL2 proteins were localized in the cytoplasm as well as in the nucleus (). Moreover, we observed colocalization of about 45% of APPL2 ve... |
To investigate whether OPT can preferentially inhibit cell proliferation and induce cell death in cancer cells, we compared the effect of OPT on MDA-MB231 breast cancer and MCF10A untransformed human breast epithelial cells. The viability of cells treated with different concentrations of OPT was measured by MTT (3-(4,5... |
To evaluate whether MB prevents noise-induced injury to the cochlea, we designed the experiments as shown in . At first, in order to confirm the efficacy of MB pretreatment, threshold shifts were calculated based on the difference between auditory brainstem response (ABR) thresholds before and after noise exposure in e... |
transcripts were detected at high levels in testis, while at low levels in heart and kidney (). To detect the global level of ESET and H3K9me3 in spermatogenesis, qRT-PCR and western blot using multiple tissues from testis at different developmental stages were performed. The expression of ESET was gradually increasing... |
Subsets and Splits
No community queries yet
The top public SQL queries from the community will appear here once available.