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[Biochemical studies on camomile components/III. In vitro studies about the antipeptic activity of (--)-alpha-bisabolol (author's transl)]. (--)-alpha-Bisabolol has a primary antipeptic action depending on dosage, which is not caused by an alteration of the pH-value. The proteolytic activity of pepsin is reduced by 50 ... |
[Demonstration of tumor inhibiting properties of a strongly immunostimulating low-molecular weight substance. Comparative studies with ifosfamide on the immuno-labile DS carcinosarcoma. Stimulation of the autoimmune activity for approx. 20 days by BA 1, a N-(2-cyanoethylene)-urea. Novel prophylactic possibilities]. A r... |
Effect of etafenone on total and regional myocardial blood flow. The distribution of blood flow to the subendocardial, medium and subepicardial layers of the left ventricular free wall was studied in anaesthetized dogs under normoxic (A), hypoxic (B) conditions and under pharmacologically induced (etafenone) coronary v... |
Influence of a new virostatic compound on the induction of enzymes in rat liver. The virostatic compound N,N-diethyl-4-[2-(2-oxo-3-tetradecyl-1-imidazolidinyl)-ethyl]-1-piperazinecarboxamide-hydrochloride (5531) was analyzed as to its effect on the induction of tryptophan-pyrrolase and tyrosineaminotransferase in rat l... |
Pharmacological properties of new neuroleptic compounds. RMI 61 140, RMI 61 144 and RMI 61 280 are newly synthetized N-[8-R-dibenzo(b,f)oxepin-10-yl]-N'-methyl-piperazine-maleates which show interesting psychopharmacologic effects. This work contains the results of a study performed with these three compounds, in order... |
[Studies on the action of an anticholinergic agent in combination with a tranquilizer on gastric juice secretion in man]. A double-blind study with intra-individual comparisons was carried out to investigate the effects of 15 mg of (8r)-3alpha-hydroxy-8-isopropyl-1alphaH-tropanium bromide(+/-)-tropate (Sch 1000), 15 mg... |
Lysosomal hydrolases of the epidermis. I. Glycosidases. Seven distinct glycosidases (EC 3.2) have been characterized in guinea-pig epidermis. Their properties indicate them to be of lysosomal origin. The 'profile' of the epidermal glycosidases is significantly different from that reported for whole skin, the activities... |
Lysosomal hydrolases of the epidermis. 2. Ester hydrolases. Five distinct ester hydrolases (EC 3-1) have been characterized in guinea-pig epidermis. These are carboxylic esterase, acid phosphatase, pyrophosphatase, and arylsulphatase A and B. Their properties are consistent with those of lysosomal enzymes. |
A serum haemagglutinating property dependent upon polycarboxyl groups. A serum agglutinin reactive with red cells in the presence of polycarboxyl groups is reported. It is likely that this represents an additional example of the type of agglutinin previously described as agglutinating red cells in the absence of ionize... |
Effect of human erythrocyte stromata on complement activation. Stroma from either normal or PNH-like red cells is capable of inhibiting, to some extent, lysis in the sucrose test and enhancing lysis in the acidified-serum test. The same opposing effects are displayed by the exclusion peaks from Sephadex G-200 obtained ... |
The effect of o-salicylate upon pentose phosphate pathway activity in normal and G6PD-deficient red cells. The effect of the major metabolite of aspirin, namely salicylic acid, upon the pentose phosphate pathway (PPP) of normal and G6PD-deficient red cells has been studied. Salicylic acid was shown to inhibit this path... |
The effects of processing of barley-based supplements on rumen pH, rate of digestion of voluntary intake of dried grass in sheep. 1. In one experiment the effect on rumen pH of feeding with restricted amounts of whole or pelleted barley was studied. With whole barley there was little variation in rumen pH associated wi... |
Poly(8-aminoguanylic acid): formation of ordered self-structures and interaction with poly(cytidylic acid). Poly(8-aminoguanylic acid) has in neutral solution a novel ordered structure of high stability. The 8-amino group permits formation of three hydrogen bonds between two residues along the "top", or long axis, of t... |
Effect of pH on substrate and inhibitor kinetic constants of human liver alanine aminopeptidase. Evidence for two ionizable active center groups. The presence of at least two ionizable active center groups has been detected by a study of the effect of pH upon catalysis of hydrolysis of L-alanyl-beta-naphthylamide by hu... |
Formation of transient complexes in the glutamate dehydrogenase catalyzed reaction. The reaction of glutamate dehydrogenase and glutamate (gl) with NAD+ and NADP+ has been studied with stopped-flow techniques. The enzyme was in all experiments present in excess of the coenzyme. The results indicate that the ternary com... |
Human brain and placental choline acetyltransferase: purification and properties. Choline acetyltransferase (EC 2.3.1.6) catalyzes the biosynthesis of acetylcholine according to the following chemical equation: acetyl-CoA + choline in equilibrium to acetylcholine + CoA. In addition to nervous tissue, primate placenta i... |
Stabilization of the globular structure of ferricytochrome c by chloride in acidic solvents. Increasing concentrations of chloride were found to increase the resolution between two visible absorbance spectral transitions associated with acidification of ferricytochrome c. Analysis of a variety of spectral and viscosity... |
A competitive labeling method for the determination of the chemical properties of solitary functional groups in proteins. The properties of the functional groups in a protein can be used as built-in-probes of the structure of the protein. We have developed a general procedure whereby the ionization constant and chemica... |
Modification of arginine and lysine in proteins with 2,4-pentanedione. Primary amines react with 2,4-pentanedione at pH 6-9 to form enamines, N-alkyl-4-amino-3-penten-2-ones. The latter compounds readily regenerate the primary amine at low pH or on treatment with hydroxylamine. Guanidine and substituted guanidines reac... |
The origin of the alkaline inactivation of pepsinogen. Above pH 8.5, pepsinogen is converted into a form which cannot be activated to pepsin on exposure to low pH. Intermediate exposure to neutral pH, however, returns the protein to a form which can be activated. Evidence is presented for a reversible, small conformati... |
Bovine liver dihydrofolate reductase: purification and properties of the enzyme. A purification procedure is reported for obtaining bovine liver dihydrofolate reductase in high yield and amounts of 100-200 mg. A key step in the procedure is the use of an affinity gel prepared by coupling pteroyl-L-lysine to Sepharose. ... |
Purification and properties of Escherichia coli dihydrofolate reductase. Dihydrofolate reductase has been purified 40-fold to apparent homogeneity from a trimethoprim-resistant strain of Escherichia coli (RT 500) using a procedure that includes methotrexate affinity column chromatography. Determinations of the molecula... |
The influence of pH on the interaction of inhibitors with triosephosphate isomerase and determination of the pKa of the active-site carboxyl group. Ionization effects on the binding of the potential transition state analogues 2-phosphoglycolate and 2-phosphoglycolohydroxamate appear to be attributable to the changing s... |
Monoanion inhibition and 35Cl nuclear magnetic resonance studies of renal dipeptidase. Kinetic analyses of monoanion inhibition and 15Cl nuclear magnetic resonance at 5.88 MHz were employed to study monoanion interactions with the zinc metalloenzyme, renal dipeptidase. The enzyme-catalyzed hydrolysis of glycyldehydroph... |
The interaction of bovine erythrocyte superoxide dismutase with hydrogen peroxide: inactivation of the enzyme. Bovine erythrocyte superoxide dismutase was slowly and irreversibly inactivated by hydrogen peroxide. The rate of this inactivation was directly dependent upon the concentrations of both H2O2 and of enzyme, an... |
Circular dichroism and fluorescence studies of homogeneous antibodies to type III pneumococcal polysaccharide. The near-ultraviolet circular dichroism (CD) of three homogeneous anti-type III pneumococcal antibodies in the absence and the presence of the specific hexasaccharide ligand was studied. In addition recombinat... |
Conformational changes induced in a homogeneous anti-type III pneumococcal antibody by oligosaccharides of increasing size. The circular polarization of luminescence (CPL) emitted by tryptophan residues was used as a sensitive probe for measuring ligand-induced structural changes in a homogeneous type III pneumococcal ... |
Evidence of the involvement of a 50S ribosomal protein in several active sites. The functional role of the Bacillus stearothermophilus 50S ribosomal protein B-L3 (probably homologous to the Escherichia coli protein L2) was examined by chemical modification. The complex [B-L3-23S RNA] was photooxidized in the presence o... |
The interaction of phospholipase A2 with micellar interfaces. The role of the N-terminal region. The localization of the previously postulated interface recognition site (IRS) in porcine pancreatic phospholipase A2, required for a specific interaction between the enzyme and organized lipid-water interfaces, was investi... |
Phospholipase A2 as a probe of phospholipid distribution in erythrocyte membranes. Factors influencing the apparent specificity of the reaction. The action of snake venom phospholipases A2 in intact human erythrocytes was investigated in detail. The basis phospholipase from Agkistrodon halys blomhifii was found to indu... |
Subunit interactions in yeast glyceraldehyde-3-phosphate dehydrogenase. The spontaneous inactivation of yeast glyceraldehyde-3-phosphate dehydrogenase was found to fit a simple two-state model at pH 8.5 and 25 degrees. The first step is a relatively rapid dissociation of the tetramer to dimers with the equilibrium larg... |
Kinetic light scattering studies on the dissociation of hemoglobin from Lumbricus terrestris. The kinetics of the pH-induced dissociation of the 3 X 10(6) mol wt hemoglobin from Lumbricus terrestris (the earthworm) have been studied in a light-scattering stopped-flow apparatus. The ligand dependent dissociation data we... |
The reversible reduction of horse metmyoglobin by the iron(II) complex of trans-1,2-diaminocyclohexane-N,N,N,n-tetraacetate. The reduction of metmyoglobin by the iron(II) complex of trans-1,2-diaminocyclohexane-N,N,N'N'-tetraacetate (FeCDTA2-) has been investigated. The equilibrium constant, measured spectrophotometric... |
Constitution and properties of axonal membranes of crustacean nerves. The purification of axonal membranes of crustaceans was followed by measuring enrichment in [3H]tetrodotoxin binding capacity and in Na+, K+-ATPase activity. A characteristic of these membranes is their high content of lipids and their low content of... |
Regulation of nitrogen fixation. Nitrogenase-derepressed mutants of Klebsiella pneumoniae. 1. A new procedure is described for selecting nitrogenase-derepressed mutants based on the method of Brenchley et al. (Brenchley, J.E., Prival, M.J. and Magasanik, B. (1973) J. Biol. Chem. 248, 6122-6128) for isolating histidase-... |
The reaction between the superoxide anion radical and cytochrome c. 1. The superoxide anion radical (O2-) reacts with ferricytochrome c to form ferrocytochrome c. No intermediate complexes are observable. No reaction could be detected between O2- and ferrocytochrome c. 2. At 20 degrees C the rate constant for the react... |
Identification of the 120 mus phase in the decay of delayed fluorescence in spinach chloroplasts and subchloroplast particles as the intrinsic back reaction. The dependence of the level of this phase on the thylakoids internal pH. After a 500 mus laser flash a 120 mus phase in the decay of delayed fluorescence is visib... |
Light-induced changes of absorbance and electron spin resonance in small photosystem II particles. Photosystem II reaction center components have been studied in small system II particles prepared with digitonin. Upon illumination the reduction of the primary acceptor was indicated by absorbance changes due to the redu... |
Enzymic reactions of fatty acid hydroperoxides in extracts of potato tuber. II. Conversion of 9- and 13-hydroperoxy-octadecadienoic acids to monohydroxydienoic acid, epoxyhydroxy- and trihydroxymonoenoic acid derivatives. 1. Crude extracts and partially purified enzyme preparations from potato tubers catalyse, at pH 5-... |
Partial purification and properties of microsomal phosphatidate phosphohydrolase from rat liver. Microsomal phosphatidate phosphohydrolase (phosphatidate phosphatase EC 3.1.3.4) was solubilized and fractionated to yield at least two distinct enzymatically active fractions. One, denoted FA, was non-specific, had a relat... |
Bile acids. XLVII. 12alpha-Hydroxylation of precursors of allo bile acids by rabbit liver microsomes. Rabbit liver microsomal preparations fortified with 0.1 mM NADPH effectively promote hydroxylation of [3beta-3H]- or [24-14C]allochenodeoxycholic acid or [5alpha,6alpha-3H2]5alpha-cholestane-3alpha,7alpha-diol to their... |
Partial purification and properties of a phenobarbital-induced aldehyde dehydrogenase of rat liver. Properties of the phenobarbital induced cytoplasmic aldehyde dehydrogenase (EC 1.2.1.3) have been studied in rat liver. 7-12-Fold higher levels were seen in the cytoplasmic activities after phenobarbital treatment in rea... |
Cholinesterases from plant tissues. VI. Preliminary characterization of enzymes from Solanum melongena L. and Zea mays L. Enzymes capable of hydrolyzing esters of thiocholine have been assayed in extracts of Solanum melongena L. (eggplant) and Zea Mays L. (corn). The enzymes from both species are inhibited by the anti-... |
Behavior of soluble and immobilized acid phosphatase in hydro-organic media. The hydrolysis of p-nitrophenyl phosphate by wheat germ acid phosphatase (orthophosphoric monoester phosphohydrolase, EC 3.1.3.2) has been investigated in mixtures of aqueous buffers with acetone, dioxane and acetonitrile. The enzyme was eithe... |
Purification and some enzymatic properties of the chitosanase from Bacillus R-4 which lyses Rhizopus cell walls. A strain of Bacillus sp (Bacillus R-4) produces a protease and a carbohydrolase both of which have the ability to lyse Rhizopus cell walls. Of the enzymes, the carbohydrolase has been purified to an ultracen... |
Specificity studies on alpha-mannosidases using oligosaccharides from mannosidosis urine as substrates. Oligosaccharides containing terminal non-reducing alpha(1 leads to 2)-, alpha(1 leads to 3)-, and alpha(1 leads to 6)-linked mannose residues, isolated from human and bovine mannosidosis urines were used as substrate... |
Calcium-stimulated adenosine triphosphatase in the microsomal fraction of tooth germ from porcine fetus. The characterization and localization of a Ca(2+)-ATPase (ATP phosphohydrolase, EC 3.6.1.3) in the tooth germ of the porcine fetus are reported. This enzyme, a microsome fraction, is preferentially activated by Ca(2... |
Preparation and characterization of an enzymatically active immobilized derivative of myosin. Purified skeletal muscle myosin (EC 3.6.1.3) has been covalently bound to Sepharose 4B by the cyanogen bromide procedure. The resulting complex, Sepharose-Myosin, possesses adenosine triphosphatase activity and is relatively s... |
Radioactive labeling and location of specific thiol groups in myosin from fast, slow and cardiac muscles. 1. Based on incorporation of radioactively labeled N-ethylmaleimide, the readily reactive thiol groups of isolated myosin (EC 3.6.1.3) from fast, slow and cardiac muscles could be classified into 3 types. All 3 myo... |
Yeast glutathione reductase. Studies of the kinetics and stability of the enzyme as a function of pH and salt concentration. 1. The pH dependencies of the apparent Michaelis constant for oxidized glutathione and the apparent turnover number of yeast glutathione reductase (EC 1.6.4.2) have been determined at a fixed con... |
Characteristics of the dephosphorylated form of phosphorylase purified from rat liver and measurement of its activity in crude liver preparations. The phosphorylated form of liver glycogen phosphorylase (alpha-1,4-glucan : orthophosphate alpha-glucosyl-transferase, EC 2.4.1.1) (phosphorylase a) is active and easily mea... |
Multiple forms of casein kinase from rabbit erythrocytes. Two rabbit erythrocyte casein kinases, GTP:casein kinase I and GTP:casein kinase II, have been purified 29 000- and 47 000-fold, respectively. Studies employing sucrose density gradient centrifugation indicate that kinase I has a molecular weight of about 9.5 - ... |
Kinetic studies and effects of anions on creatine phosphokinase from skeletal muscle of rhesus monkey (Macaca mulatta). A purification procedure for creatine kinase (EC 2.7.3.2) from muscle of the monke35--170 muequiv H+/mg protein per min at 30 degrees C and a yield of approx. 0.5 g/kg muscle. Assuming equilibrium kin... |
Studies on rat renal cortical cell kallikrein. I. Separation and measurement. A technique has been developed to separate and measure kallikrein in a heterogeneous population of rat renal cortical cells in suspension. After rat kidneys were perfused in situ in anaesthetized rats, viable, counted cortical cell suspension... |
Studies on electron transfer between mercury electrode and hemoprotein. The electrochemical behaviour of ferricytochrome c, metmyoglobin and methemoglobin was studied using d.c., a.c. and differential pulse polarography, and controlled potential electrolysis. 1. The three hemoproteins yield d.c. polarographic steps, an... |
The binding of organic phosphates to human methaemoglobin A. Perturbation of the polymerization of proteins by effectors. Theory is presented relating to the binding of an effector to two states of a protein acceptor coexisting in equilibrium. The problem is treated in terms of the four possible cases which specify rel... |
N-terminal spin label studies of hemoglobin, Ligand and pH dependence. Human hemoglobin was spin labeled with 4-isothiocanato-2,2,6,6-tetramethyl-piperdinooxyl, which is known to bind specifically to the N-terminal alpha-amino groups of proteins and slightly to the reactive sulfhydryl groups. Electron spin resonance (E... |
The behavior of holo- and apo-forms of bovine superoxide dismutase at low pH. 1. Holo-superoxide dismutase from bovine erythrocytes has been shown to undergo a reversible structural modification in the pH 3-5 range. 2. The spectral alterations observed on changing from neutrality to pH 2 were: a slight attenuation of t... |
Mechanical precipitation of hemoglobin köln. Hb Köln (beta 98 Val leads to Met) was found to precipitate rapidly during mechanical shaking. The rate of precipitation of Hb Köln is 5-6 times faster than that of Hb S. The kinetics of precipitation of the patient's hemolysate, which is a mixture of Hb Köln and Hb A, showe... |
Physical properties and subunits of Haemopis grandis erythrocruorin. The erythrocruorin of the leech Haemopis grandis possessed an S20,w of 57 S at neutral pH, its isoelectric point at pH 6.0 and exhibited a slightly sigmoid oxygenation curve with n approximately 2.1 and P50 = 11.2 mm at pH 7.4. A minimum molecular wei... |
Myosin from arterial smooth muscle: isolation following actin depolymerization. The contractile proteins from arterial smooth muscle are highly soluble, and can be extracted at I = 0.05. However, they can be precipitated by a prolonged dialysis at pH 6 to give an actomyosin with a high, although variable, actin:myosin ... |
Hybrids of chemical derivatives of Escherichia coli alkaline phosphatase. The activities of hybrid dimers of alkaline phosphatase containing two chemically modified subunits have been investigated. One hybrid species was prepared by dissociation and reconstitution of a mixture of two variants produced by chemical modif... |
Some physicochemical properties of hemoglobin-manitoba (alpha2 102Ser replaced by Arg (G9) beta2). Hb-Manitoba was discovered in 1970 [1] in a Canadian family of British origin. Recently we observed the same variant in a second family, and found that the oxy-derivative of Hb-Manitoba is slightly unstable at 65 degrees ... |
The oxygen affinity of haemoglobin Tak, a variant with an elongated beta chain. The oxygen affinity was investigated of purified Hb Tak, a human haemoglobin variant with elongated beta-chains. A very low P50 value was found which was not influenced by the addition of 2,3 diphosphoglycerate. The n value was 1, indicatin... |
Effects of membrane ribonuclease and 3'-nucleotidase on the digestion of polyuridylic acid by rat liver plasma membrane. 1. Fragments of isolated rat liver plasma membrane possess a ribonuclease activity which at pH 7.8 in the presence of 10 mM EDTA can digest polyuridylic acid (poly(U)) and polycytidylic acid (poly(C)... |
Alterations in phospholipid-dependent (Na+ +K+)-ATPase activity due to lipid fluidity. Effects of cholesterol and Mg2+. The (Na+ +K+)-activated, Mg2+-dependent ATPase from rabbit kidney outer medulla was prepared in a partially inactivated, soluble form depleted of endogenous phospholipids, using deoxycholate. This pre... |
Conformational and molecular responses to pH variation of the purified membrane adenosine triphosphatase of Micrococcus lysodeikticus. A preparation of ATPase from the membranes of Micrococcus lysodeikticus, solubilized and more than 95% pure, showed two main bands in analytical polyacrylamide gel electrophoresis. They... |
Membrane-bound enzymes. III. Protease activity in leucocytes in relation to erythrocyte membranes. Protease activity was detected in membranes of human bovine erythrocytes prepared by the conventional procedures which include washing and removal of the "buffy layer". The enzyme was extracted by 0.75 M KCNS or (NH4)2SO4... |
[Structure formation in interphase adsorption layers of lysozyme at liquid boundaries]. In connection with the modelling of biomembranes regularities of the formation and development of interphase adsorption layers of lysozyme at liquid borders under different conditions and depending on the nature of carbohydrate phas... |
Polymer concentration dependence of the helix to random coil transition of a charged polypeptide in aqueous salt solution. The helix to coil transition of poly(L-glutamic acid) was investigated in 0.05 and 0.005 M aqueous potassium chloride solutions by use of potentiometric titration and circular dichroism measurement... |
[Relation between fluorescence and circular dichroism of the complex of the fluorescence probe 4-dimethylaminochalcone with serum albumin]. The fluorescence probe(4-dimethylaminochalcone; DMH) was noncovalently linked to human serum albumin (HSA). The variation of pH was due to serum albumin structural changes, which w... |
[Luminescence study of the effect of temperature on the conformational state of fibrinogen]. Results are presented of measuring fibrinogen fluorescence parameters in temperature range of 20-80 degrees C at different pH of the solution. It was found that the temperature increase from 20 to 40 degrees C for solutions wit... |
[Combined effects of hypoxia and hypercapnia on the functional state of the respiratory center]. Experiments were conducted on cats under nembutal anesthesia; a study was made of pulse activity of bulbar respiratory neurons, electrical activity of the diaphragm and of the intercostal muscles; pO2, pCO2, pH, arterial bl... |
[Level of nicotinamide coenzymes in the liver and myocardium of rats poisoned with dichlorethane]. Experiments were conducted on male rats. A study was made of the content of nicotinamide coenzymes in the liver and myocardium 24 hours after the administration of 0.5 ml of dichloroethane into the stomach. In parallel wi... |
[Effect of carbidine on conditioned defense reflexes]. Chronic experiments were conducted on rats and rabbits; a study was made of the effect of carbidine on the conditioned defence reflexes in stimulation of the mesencephalic part of the reticular formation. Carbidine prevented the depression of the conditioned defenc... |
[Level of nicotinamide coenzymes in the myocardium of rats during the effects of methylxanthines (theophylline, theobromine, caffeine) and catecholamines]. It was shown in acute experiments on rats that one hour after an intraperitoneal injection of theophylline (50 mg/kg) there was a decrease in the NAD + NADP content... |
Potable water quality in rural Georgetown County. Drinking water supplies of 161 rural communities, in Georgetown County, South Carolina, were randomly selected for sample collection. The analysis showed that most of the waters were slightly acidic. Low, but acceptable concentrations of chloride, copper, fluoride, sodi... |
The importance of an innervated and intact antrum and pylorus in preventing postoperative duodenogastric reflux and gastritis. This study has investigated the relationship between duodenogastric reflux, gastritis and certain symptoms 6-12 months after three operations for uncomplicated duodenal ulcer. The operations st... |
Haemoglobin Rahere (beta Lys-Thr): A new high affinity haemoglobin associated with decreased 2, 3-diphosphoglycerate binding and relative polycythaemia. A new haemoglobin with increased oxygen affinity, beta82 (EF6) lysine leads to threonine (Hb Rahere), was found during the investigation of a patient who was found to ... |
Endoscopic papillotomy and removal of gall stones. Endoscopic papillotomy was attempted in 59 patients with extrahepatic obstruction of the biliary duct system and was actually performed in 50 patients. A special high-frequency diathermy knife was introduced via a duodenoscope into the terminal common bile duct and the... |
Inhibitory postsynaptic actions of taurine, GABA and other amino acids on motoneurons of the isolated frog spinal cord. The actions of glycine, GABA, alpha-alanine, beta-alanine and taurine were studied by intracellular recordings from lumbar motoneurons of the isolated spinal cord of the frog. All amino acids tested p... |
The involvement of lysophosphoglycerides in neurotransmitter release; the composition and turnover of phospholipids of synaptic vesicles of guinea-pig cerebral cortex and Torpedo electric organ and the effect of stimulation. (1) Crude synaptosomal fractions (P2) derived from guinea-pig cerebral cortex were incubated in... |
Cardiac output response to altered acid-base status during diethyl ether anaesthesia. The effects of acid-base changes on cardiac output during diethyl ether anaesthesia were studied in 25 mongrel dogs prepared by surgically implanting a plastic encased non-ferrous core electromagnetic probe on the ascending aorta. The... |
The prevention of autolysis of stored cornea using steroid as a lysosome membrane stabilizer. Many eyes donated for use in corneal grafting are rejected because of signs of autolysis in the donor material. The purpose of this experimental study was to determine whether hydrocortisone acting as a lysosome membrane stabi... |
Respiratory effects of H+ and dinitrophenol injections into the brain stem subarachnoid space of fetal lambs. Mock cerebrospinal fluid (pH 5.37-8.38) or 2,4-dinitrophenol (DNP) (0.15-1.5 mg) was injected into the subarachnoid space of the ventral brain stem of exteriorized fetal sheep. Changes in pH on the ventral surf... |
Soil fungistasis: elevation of the exogenous carbon and nitrogen requirements for spore germination by fungistatic volatiles in soils. Axenic, washed conidia of Fusarium solani f. sp. phaseoli, Aspergillus flavus, and Verticillium albo-atrum were placed on washed Difco purified agar discs along with an inorganic salt s... |
Nitrofurazone-reducing enzymes in E. coli and their role in drug activation in vivo. Earlier work showed that Escherichia coli contains at least two enzymes which reduce nitrofurazone and other nitrofuran derivatives. One of these enzymes is lacking in some nitrofurazone-resistant mutant strains. We now report that the... |
Enzymatic hydrolysis of agar: purification and characterization of neoagarobiose hydrolase and p-nitrophenyl alpha-galactoside hydrolase. The mixture of polysaccharides in the gelling component of agar (agarose) is hydrolyzed to D-galactose and 3,6-anhydro-L-galactose by a series of hydrolytic enzymes obtained from Pse... |
Temperature and pH optima for 21 species of thermophilic and thermotolerant fungi. A glucose-containing mineral medium supplemented with 0.01% yeast extract is described upon which all the species of thermophilic and thermotolerant fungi tested will grow. Thirteen of the 21 species do not require the yeast extract supp... |
Physiological differences among isolates of Phytophthora cinnamomi. Significant differences in amylase, beta-glucosidase, and phosphatase activities were observed among four Phytophthora cinnamomi isolates grown in nutrient-amended sterilized soil for 20 days. Amylase pH optima for the four isolates were within a relat... |
Regulation and properties of an invertase from Clostridium pasteurianum. An intracellular invertase was induced in cultures of Clostridium pasteurianum utilizing sucrose as its carbon source for growth. This enzyme synthesis could be repressed by the addition of fructose of a sucrose-growing culture. In contrast, inver... |
Characterization studies on the membrane-bound adenosine triphosphatase (ATPase) of Azotobacter vinelandii. The adenosinetriphosphatase (ATPase) (EC 3.6.1.3) activity in Azotobacter vinelandii concentrates in the membranous R3 fraction that is directly associated with Azotobacter electron transport function. Sonically ... |
Effects of zinc-smelter emissions on forest soil microflora. Within 2 km of a zinc (Zn) smelter in Palmerton, Pennsylvania, near the Lehigh Water Gap, up to 13.5% Zn by weight has been measured in the O2 horizon of the soil, and up to 8% Zn in the A1 horizon. The total numbers of bacteria, actinomycetes, and fungi (mea... |
alpha-Naphthoflavone activation of 6-hydroxymethylbenzo(alpha)pyrene synthetase. alpha-Naphthoflavone activates the aryl hydroxymethyl synthetase of both the microsomal membrane-bound and soluble enzymes of rat liver and rat lung. The enzyme catalyzes the hydroxymethylation of benzo(alpha)pyrene to the 6-hydroxymethyl ... |
The effects of the continuous administration of N,N-dimethyl-4-phenylazoaniline (DAB) on the activities and the inducibilities of some drug-metabolizing enzymes in rat liver. (1) The effect of feeding a relatively low-protein diet containing 0.06% DAB for 29 weeks on the activity of DAB-azoreductase, nitroreductase (p-... |
Some characteristics of two azoreductase systems in rat liver. Relevance to the activity of 2-[4'-di(2"-bromopropyl)-aminophenylazo]benzoic acid (CB10-252), a compound possessing latent cytotoxic activity. The system involved in the reduction of 2-[4'-di(2''-bromopropyl) aminophenylazolbenzoic acid (CB10-252), an agent... |
Mercury inhibition of avian fatty acid synthetase complex. (1) Subcutaneous or intra-abdominal injections of 8 mg of HgCl2/100 g body weight markedly depressed hepatic fatty acid synthetase activity of chicks at 1 h post-injection. The depression occurred despite the fact that the chicks continued to eat up until the t... |
[Basic phospholipase of Naja nigricollis venom]. It is confirmed that N. nigricollis venom contains several phospholipases one of these is a basic phospholipase A. This enzyme is toxic for mice when injected intravenously. In vitro it reacts on egg yolk lecithin producing lysolecithin and prevents the phenomenon of blo... |
[Spontaneous mortality and vascular lesions in 3 rat strains with different blood pressure levels]. We have observed a high and significant mortality in spontaneously hypertensive rats compared to normotensive and hypotensive controls, in the fifth generation. The hypertensive rats exhibited a high frequency of cerebra... |
Effects of decreasing arterial blood pressure on cerebral blood flow in the baboon. Influence of the sympathetic nervous system. The influence of the sympathetic nervous system on the cerebral circulatory response to graded reductions in mean arterial blood pressure was studied in anesthetized baboons. Cerebral blood f... |
TransCorpus-bio
TransCorpus-bio is a large-scale, parallel biomedical corpus consisting of PubMed abstracts. This dataset is used in the TransCorpus Toolkit and is designed to enable high-quality multi-lingual biomedical language modeling and downstream NLP research.
Currently Translated with TransCorpus Toolkit
- In French : TransCorpus-bio-fr 🤗
- In Spanish : TransCorpus-bio-es 🤗
Dataset Details
- Source: PubMed abstracts (English)
- Size: 22 million abstracts, 30.2GB of text
- Domain: Biomedical, clinical, life sciences
- Format: one abstract per line
Motivation
Non-English languages are low-resource languages for biomedical NLP, with limited availability of large, high-quality corpora. TransCorpus-bio bridges this gap by leveraging state-of-the-art neural machine translation to generate a massive, high-quality synthetic corpus, enabling robust pretraining and evaluation of Spanish biomedical language models.
from datasets import load_dataset
dataset = load_dataset("jknafou/TransCorpus-bio", split="train")
print(dataset)
# Output:
# Dataset({
# features: ['text'],
# num_rows: 21567136
# })
print(dataset[0])
Benchmark Results in our French Experiment
TransBERT-bio-fr pretrained on TransCorpus-bio-fr achieve state-of-the-art results on the French biomedical benchmark DrBenchmark, outperforming both general-domain and previous domain-specific models on classification, NER, POS, and STS tasks. See TransBERT-bio-fr for details.
Why Synthetic Translation?
- Scalable: Enables creation of large-scale corpora for any language with a strong MT system.
- Effective: Supports state-of-the-art performance in downstream tasks.
- Accessible: Makes domain-specific NLP feasible for any languages.
Citation
If you use this corpus, please cite:
@inproceedings{knafou-etal-2025-transbert,
title = "{T}rans{BERT}: A Framework for Synthetic Translation in Domain-Specific Language Modeling",
author = {Knafou, Julien and
Mottin, Luc and
Mottaz, Ana{\"i}s and
Flament, Alexandre and
Ruch, Patrick},
editor = "Christodoulopoulos, Christos and
Chakraborty, Tanmoy and
Rose, Carolyn and
Peng, Violet",
booktitle = "Findings of the Association for Computational Linguistics: EMNLP 2025",
month = nov,
year = "2025",
address = "Suzhou, China",
publisher = "Association for Computational Linguistics",
url = "https://aclanthology.org/2025.findings-emnlp.1053/",
doi = "10.18653/v1/2025.findings-emnlp.1053",
pages = "19338--19354",
ISBN = "979-8-89176-335-7",
abstract = "The scarcity of non-English language data in specialized domains significantly limits the development of effective Natural Language Processing (NLP) tools. We present TransBERT, a novel framework for pre-training language models using exclusively synthetically translated text, and introduce TransCorpus, a scalable translation toolkit. Focusing on the life sciences domain in French, our approach demonstrates that state-of-the-art performance on various downstream tasks can be achieved solely by leveraging synthetically translated data. We release the TransCorpus toolkit, the TransCorpus-bio-fr corpus (36.4GB of French life sciences text), TransBERT-bio-fr, its associated pre-trained language model and reproducible code for both pre-training and fine-tuning. Our results highlight the viability of synthetic translation in a high-resource translation direction for building high-quality NLP resources in low-resource language/domain pairs."
}
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