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Intrarenal infusion of somatostatin in anesthetized dogs produced a prompt increase in urine flow in association with a decrease in urinary osmolality and an increase in free water clearance. These changes occurred in the absence of changes in arterial pressure, renal plasma flow, osmolar clearance, electrolyte excreti... | US |
The objective of this study was to investigate changes in the secretion of prolactin (PRL) and growth hormone (GH) occurring during a 24 h period in the mouse. Adult female mice of the C57BL/St strain and male mice of the C3H/St strain, maintained on a 14 h light and 10 h dark schedule, were used. Serum and pituitary c... | US |
Immunoelectrophoresis revealed in phenol extracts from S. faecalis and S. faecium a mixture of free and lipid-bound teichoic acids, both reactive with Group D antisera. In phenol extracts from S. suis only lipid-bound teichoic acid, also reactive with Group D antiserum, was seen. This difference probably accounts for t... | US |
Graft-vs.-host (GVH) reactivity of parental lymph node (LN) cells was assayed by measurements of 3H-thymidine incorporation in vivo in spleens of irradiated F1 recipients. Preincubation of parental LN cells with vesicular stomatitis virus (VSV) for 2 h at 37 degrees C followed by washing resulted in an 85-90% reduction... | US |
Frequencies of different staphylococcal nasal carrier classes were studied in groups of students of nursing and of laboratory personnel in Cologne, West Germany, and in Krakow, Poland, by six consecutive samplings of their nasal vestibules. The general number of persistent carriers of S. aureus appeared to be lower tha... | US |
A DNA replication mutant of yeast, cdc8, was found to decrease UV-induced reversion of lys2-1, arg4-17, tyr1 and ura1. This effect was observed with all three alleles of cdc8 tested. Survival curves obtained following UV irradiation in cdc8 rad double mutants show that cdc8 is epistatic to rad6, as well as to rad1; cdc... | US |
Assuming that unrecognized or inadequately corrected hypovolemia results in higher mortality and morbidity rates, we developed a systematic approach to resuscitation that would: 1) identify criteria to aid in the recognition of hypovolemia and ensure the expeditious correction of this defect without interfering with di... | US |
Variation in the assessment of basic clinical data gathered by emergency medical technicians and emergency department nurses was studied. Prior to testing, precise definitions, categories and procedures were developed and tolerance limits for the quantitative variables were created. Each participant evaluated four cons... | US |
There is a need at both the emergency department and national level for a systematic method to prioritize clinical and nonclinical areas for research and quality assessment. A method has been developed and applied that allows frequently presenting diagnoses and management issues to be rated in terms of morbidity genera... | US |
A hypertonic albuminated fluid demand regimen (HALFD) for resuscitation has been used in burn patients since January 1, 1976. The effects of the HALFD method were compared with hypertonic fluid and Ringer's lactate resuscitation. Specific attention was directed to fluid, colloid, and volume changes. Resuscitation was g... | US |
A karyological analysis of the G-banded chromosomes of the serially transplantable GW-39 human colonic carcinoma was undertaken. GW-39 cells harvested from hamsters, nude (athymic) mice and cell cultures from 1972 to 1976 revealed a hypodiploid stemline of 45 human chromosomes, with a consistent loss of a 21-chromosome... | US |
The postulated role of macrophages in the primary infection of human lymphocytes by Epstein-Barr virus (EBV) was examined. Macrophage removal had no effect on the blastogenic response but slightly reduced the percentage of EBNA-positive cells induced by the B 95-8 virus strain. Both the rate of DNA synthesis and the ap... | US |
Protein modification with dimethylnitrosamine was studied in vitro in the presence of hamster liver microsomal fraction. Incorporation of radioactive methyl groups from dimethylnitrosamine into the exogenously added protein was dependent on the microsomal mixed function oxidase system. The methylation yielded chemicall... | US |
Phorbolol myristate acetate, a metabolite of the tumor promotor phorbol muristate acetate in mouse skin, has one-fiftieth the potency of the parent molecule for the induction of cell division in stationary cultures of BALB/c-3T3 mouse embryo cells. Similarly, in a mixed cell culture assay devised for detection of tumor... | US |
Binding capacities of membrane suspensions and dissolved compounds for mercurials were titrated by a new potentiometric method. Critical steps included a silver electrode of new design, the use of L-cysteine as a thiol buffer, a nitrogen atmosphere, and pretreatment of samples with equimolar mercurial and cysteine. Tit... | US |
The effect of semistarvation on small intestinal transport of D-glucose, L-valine, and NaCl was studied in an in vitro system of isolated rat brush border membrane vesicles. Whereas semistarvation enhanced the transport rate for L-valine by 19-29%, there was no change in D-glucose transport. When energy in the form of ... | US |
Amino acid transport systems for alanine and leucine have been reconstituted into artificial lipid vesicles. Purified plasma membrane vesicles from Ehrlich ascites cells were dissolved in 2% sodium cholate, 1 mM dithiothreitol, 0.5 mM EDTA, a mixture which solubilized approximately 50% of the membrane protein. This sol... | US |
A major glycoprotein fraction of the isolated adipocyte plasma membrane migrated in the 200,000-dalton region of dodecyl sulfate polyacrylamide gels following solubilization in 2% dodecyl sulfate/4M urea at room temperature in the absence of reductant. Limited heat treatment allowed resolution of the glycoprotein into ... | US |
A new economical and reproducible micromethod for the preparation of human macrophage cultures in wells of a microtiter plate is described. The technique has been employed for the study of events which occur in the interaction of lymphocytes with macrophages in PHA-stimulated immune interferon production and blastogene... | US |
The chronic administration of Tilorone [(2,7-bis(diethylamino)ethoxy]fluoren-9-one) with antigen seems to prevent the generation of DTH effector cells. The administration of Tilorone proximal to DTH challenge blocks the expression of DTH. By local transfer of DTH effector cells, this latter effect can be shown to be du... | US |
Tilorone (2,7-bis[(diethylamino)ethoxy]fluoren-9-one) can enhance the humoral immune response of mice to SRBC although Tilorone has no effect on functional activity of splenic B cells in the absence of immunogen. Tilorone does not alter the effective level of T cell help but does seem to alter the level of effective su... | US |
The effects of chemotactic factors on rabbit neutrophils were evaluated measuring cell migration in modified Boyden chambers and under agarose, in lysosomal enzyme release, leukocyte aggregation, and in vivo neutropenia. Chemotactins employed included the complement-derived C3 and C5 fragments, the bacterial chemotacti... | US |
Histamine or prostaglandin (PG) E1 or E2 administered to rabbits topically alone in high doses produced conjunctival vasodilation associated with little or no edema while their mixture at lower concentrations produced conjunctival vasodilation associated with profound edema. Sections of tissues treated with the mixture... | US |
The accumulation of cyclic adenosine 3',5'-monophosphate (cAMP) in guinea-pig macrophages exposed to the adenylate cyclase (AC) stimulators prostaglandin E1 (PGE1) and isoproterenol (IP), was markedly enhanced by pretreatment of the cells with colchicine, vinblastine, and podophyllotoxin--agents which prevent microtubu... | US |
Mouse amniotic fluid was shown to contain a noncytotoxic inhibitor of primary gammaM and secondary gammaM, gammaG subclass splenic plaque forming cells in vitro to SRBC. The suppressive effect was not abolished by exhaustive dialysis or by absorption of mouse amniotic fluid (MAF) with SRBC. Polyacrylamide gel analysis ... | US |
A two-phase model of allograft immunity was studied. In the first phase, specific immune T-cells were generated by incubation of responder cells with mitomycin-C treated allogeneic stimulator cells of the same H-2 type as mouse mastocytoma tumor cells. In the second step, the ability of the sensitized cells to kill Cr5... | US |
Surface-bound alpha-fetoprotein (AFP) was demonstrated by immunofluorescence on approximately 1/3 of splenic lymphocytes in chronic murine graft vs. host (GVH) reactions. Splenic lymphocytes were also shown to have a suppressed phytohemagglutinin (PHA) response compared to controls while lymph node cells from the same ... | US |
AFP is one of several oncofetal proteins synthesized in large amounts by the fetus. Although synthesis drops markedly shortly after birth, small amounts of AFP continue to be produced in the adult. The function of AFP is unknown, but recent studies suggest the possibility that it may have immunoregulatory properties an... | US |
Chlorinated hydrocarbons, such as the pesticide lindane (gamma-hexachlorocyclohexane), quench the fluorescence of carbazole. The observed quenching is a result of the molecular contacts which occur upon diffusional collisions. Because the amount of quenching depends upon the collisional frequency between carbazole and ... | US |
Phosphate-buffered saline (PBS) soluble extracts of human epidermis and dermis from secretor donors contain products with blood group antigenic activity. The predominant blood group in a sample was the same as the red cell phenotype of the donor. H substance activity was present in all PBS-soluble products (from A, B a... | US |
The relation of the hippocampal EEG to behavior and to the neocortical EEG is being studied in psychomotor epileptics. Hippocampal recordings displaying only rare epileptiform spikes and slow waves are found to follow grossly the simultaneously recorded neocortical EEG, becoming desynchronized during wakefulness and pa... | US |
Single and multiple unit recordings were made from fine wires stereotaxically implanted in the hippocampus (HC), hippocampal gyrus (HCG), and amygdala (Am) of psychomotor epileptics. During a series of memory and control tests presented on slides, 21 of 155 HCG units, 15 of 59 HC units, and 2 of 54 Am units showed what... | US |
Excessive daytime sleepiness is a complaint characterizing many disorders of the wakefulness--sleep cycle. This paper addresses the complaint of sleepiness objectively by an attempt to differentiate a group of control subjects from a group of patients with unambiguous narcolepsy. Fourteen control and 27 narcoleptic sub... | US |
Indirect immunofluorescence (IF) studies were performed on skin from a variety of vertebrate specimens and IgG fractions from pemphigoid and pemphigus sera. Pemphigoid antigen was present in fish, amphibian, reptilian, avian and mammalian skin, whereas pemphigus antigen was observed in avian and mammalian skin only. | US |
Extracts from dog livers which had been regenerating for 24, 48, and 72 h after hepatectomy were infused for 6 h into the left portal vein of animals which had fresh portacaval shunts (Eck fistula) and which were killed 2 and 3 days later. The brief exposure to the 48-h and especially the 72-h regenerating liver extrac... | US |
Forty narcoleptic patients were given the Multiple Sleep Latency Test, consisting of 20 min opportunities to sleep offered at 10.00, 12.00, 14.00, 16.00 and 18.00 o'clock. Eleven patients had 2 episodes of REM sleep, 5 had 3, 11 had 4, and 13 had 5 before they were awakened. Fourteen control subjects given similar oppo... | US |
Quantitative differences in the magnitude of antigen-induced proliferative responses of sensitized lymph node cells between low (C3H/Anf or C3H/Cr) and high (C3H/Hej or CBA/j) responder H-2k mice have been observed 1 to 2 weeks after in vivo sensitization with antigen (OVA, PPD, and GAT). We have shown that antigen pre... | US |
Murine amniotic fluid (MAF), alpha-foeto-protein (AFP) and MAF depleted of AFP by affinity chromatography (MAF-AFP) inhibited the T-cell dependent in vitro proliferative responses of lymph node cells sensitized to a variety of soluble antigens. Variable degrees of inhibition were observed with the different antigens us... | US |
The ability of mouse amniotic fluid (MAF), alpha-foetoprotein (AFP) and MAF depleted of AFP (MAF - AFP) to suppress primary one-way MLR's was investigated. It was found that MAF, AFP and MAF - AFP were all suppressive of MLR's specific for MHC, K, D or I + S determinants. Suppression was observed when either lymph node... | US |
Electrophoretic light scattering (laser Doppler electrophoresis) has been employed to study the effects of guinea pig IgG immune complexes on the electrophoretic mobility distributions of guinea pig resident peritoneal cells. The resident population of cells is composed of macrophages (approximately 75%) and eosinophil... | US |
Cytologic preparations made from the tracheobronchial tree taken by the Schreiber catheter have been scanned by three color microphotometry. The digitized cell images were processed by the analytical cytodiagnostic programs of the TICAS system. Cells were sorted into two control groups and five groups of increasing aty... | US |
When lymphocytes from a majority of patients with cancer are incubated with encephalitogenic factor, a lymphocyte product is released that reduces the anodic electrophoretic mobilities of guinea pig macrophages and fixed, tanned sheep erythrocytes. Although these reactions are not specific for cancer, it is distinctly ... | US |
Adhesion of leukocytes and platelets to solid substrates of different surface tensions and hence different wettability is studied from a thermodynamic point of view. A simple thermodynamic model predicts that a cellular adhesion should increase with increasing surface tension of the solid substrate if the surface tensi... | US |
T and B mouse spleen lymphocytes were separated by density gradient electrophoresis on the basis of their surface charge. In all strains examined, the T lymphocytes were found in the high mobility fractions and the B in the low. The T and B cells were separated completely in most fractions, with some overlapping in the... | US |
Lindahl first described the separation of cells by velocity sedimentation utilizing a special technique (counterstreaming centrifugation) that was later modified slightly and renamed centrifugal elutriation. Centrifugal elutriation has been applied, with variable degrees of success, to the separation of hemopoietic cel... | US |
In the influencent-flow cytophotometric measurement of cellular DNA content the DNA distributions usually have two peaks. The second peak, which corresponds to the 4C DNA content of G2 and M cells, is often positioned at lower values of DNA content than twice that of the 2C DNA peak which contains G1 cells. Computerize... | US |
Splenocytes and column-separated T cells are differentiated into subpopulations of T and B cells on the basis of computer-assisted morphometric analysis of Feulgen-positive nuclear DNA. Differentiation is based upon the analysis of computable image information related to DNA distribution patterns. The technique at the ... | US |
Splenocytes separated by physical means and classified as T cells bay immunologic tests and computerized microphotometric analysis are differentiated into subgroups by analysis of the distribution patterns of Feulgen-positive nuclear DNA. In like fashion T cells obtained as purified preparations after separation on a n... | US |
Synchronized tranformed and reverse-transformed (by 10(-3) M B2cAMP) CHO-K1 cells, growing adherent to plastic, are characterized by means of geometric and densitometric parameters at the level of both the entire cell and of the nuclei at various time intervals after selective miotic detachment. Transformed and reverse... | US |
Blue dextran has been adsorbed to millipore filter discs in a linkage stable under a variety of conditions. These discs have been to bind lactic dehydrogenase, which may then be specifically eluted with NADH, one of its substrates. In contrast, another enzyme, alkaline phosphatase, not expected to bind to blue dextran,... | US |
Four distinct alpha-fetoprotein (AFP) species were separated by ion-exchange chromatography of the approximately 70 000 dalton fraction of an extract of late-gestation fetal mice. Although chromatographicaly heterogeneous, antigenic differences among the AFP species resolved were not detected using classical double-dif... | US |
Eleven patients with short P-R intervals and narrow QRS complexes had ventricular tachycardia due to organic heart disease: mitral valve prolapse with mitral insufficiency (2 patients); alcoholic (?) cardiomyopathy (2 patients); and coronary artery disease (7 patients). Intracardiac studies showed short A-H intervals d... | US |
An objective antiglobulin radioimmunoassay for the analysis of anti-spermatozoa antisera has been used to characterize more than 30 alloantisera. Each of these antisera was raised against spermatozoa from mice carrying T/t regin mutations. Whilst all of the antisera had considerable activity for mouse spermatozoa, none... | US |
Patient reports are presented to indicate the application of standard implanted programmable pacemakers with endocardial electrodes for long-term overdrive suppression of recurrent ventricular tachycardia, and their adaptability to non-invasively induced burts of rapid ventricular pacing to cardiovert that arrhythmia. ... | US |
Loss of normal pacemaker stimulation and/or sensing functions requires prompt detection, automatic correction, and automatic and continuous "marking" of the intermittent failure. The autodiagnostic pacemaker (ADP) detects "failure to capture" (FC) by distinguishing, at its single stimulating and sensing electrode, betw... | US |
The polarization characteristics of Pt-Ir and Elgiloy small-surface-area (10-12.5 mm2) pacemaker electrodes were studied at AC linear (sensing) and DC non-linear (pacing) conditions. The electrodes' AC polarization impedance was approximately equal to the demand pacemaker's input impedance, which causes waveform distor... | US |
The frequency of lead failure requiring invasive correction in a total of 276 implants of four different transvenous leads (6907, continuous lead, IE-65-I, and MIP 2000) was observed during a one-and-one-half year period with a minimum of two months follow-up post-implant. Implants were on a successive sequential basis... | US |
A high incidence of sudden death due to ventricular fibrillation (VF) has been observed in dogs under chronic treatment with probucol, a new hypocholesterolemic agent. The present study describes the cardiac electrophysiologic properties of probucol-treated dogs and characterizes the electrophysiological response of th... | US |
A semipurified liquid diet was used to develop a chronic rat model of the fetal alcohol syndrome. Control female rats gained weight normally, reproduced normally, and gave birth to normal litters on this diet. Increased neonatal mortality, decreased neonatal weights, and altered sex ratios were observed in offspring of... | US |
Partial acid hydrolysis and methanolysis released disaccharides and disaccharide methylglycosides from the glycoproteins, ovomucoid and porcine gastric mucin in amounts of 0.5--7 microgram disaccharide per mg of glycoprotein. These disaccharides were fractionated by gas chromatography as the trimethylsilyl (Me3Si) deri... | US |
Hearing impairment and related cochlear histopathologic changes were evaluated in experimental animals after treatment with aminoglycoside antibiotics or exposure to intense sound. In the course of treatment with kanamycin, neomycin, or dihydrostreptomycin, permanent hearing loss in monkeys and guinea pigs occurred fir... | US |
Electronic parameters related to the cytochrome P450-catalyzed reactions of eight polycyclic aromatic amines have been calculated using all valence electron semiempirical molecular orbital methods. The reactions considered lead to the presumably carcinogenic arylnitrenium ions and to the competing hydroxylation and epo... | US |
Staining polarization optical techniques showed differences in the structural organization of DNA of chromatin in interphase nuclei and in mitotic chromosomes. The DNA was non-birefringent in intact interphase cell nuclei, but birefringent in chromosomes and in isolated nuclei incubated in a physiological electrolyte s... | US |
Proliferative neovascular retinopathies may be caused by the release of a hypothetical vasoproliferative factor, but mechanical factors seem to self-perpetuate the disease as well. Contraction of proliferative tissue causes vitreous detachment and traction on the retina. Congestion of vascular fronds by traction furthe... | US |
Dihalomethanes are metabolized to carbon monoxide both in vivo and in vitro. The reaction is catalyzed by a hepatic microsomal cytochrome P-450 dependent mixed function oxidase system. Bioorganic mechanism studies suggest an initial oxygen insertion reaction followed by rearrangement to a formyl halide intermediate whi... | US |
Anti-suppressor factor antisera, prepared either in rabbits (R alpha SF) or in syngeneic (CBA) mice (M alpha SF) by repeated immunisation with antigen purified CBA antigen specific suppressor factor reactive to KLH was shown to abolish the suppression caused by suppressor factors (SF) in vitro. R alpha SF inhibited the... | US |
We have surveyed the clastogenic potential of 12 different groups of stains and dyes totalling 48 compounds. We observed that 18 compounds induced significant increase in chromosome damage. Most of them were also found to be mutagenic, carcinogenic, or toxic in other reported studies. However, no significant studies we... | US |
Keratinocytes and melanocytes cultured from guinea-pig epidermis were studied with scanning, transmission and high voltage electron microscopy to characterize the surface and internal morphology. Keratinocytes exhibited contact-inhibition and a range of surface structures consistent with cell-cycle dependent changes. S... | US |
Reactivation of fetal hemoglobin gene expressions was studied in new-world monkeys (marmosets). After a series of intraperitoneal injections of D-thyroxine, fetal hemoglobin production was increased in three experimental animals. Control animals were unaffected by the injection of the solvent. Because the percentage of... | US |
Four eyes were divided into portions which were prepared by various techniques for immunofluorescence microscopy. One portion was frozen and processed by cryostat cutting, a second was fixed in alcohol-acetic acid and embedded in paraffin, and a third was soaked in saline for 2 days to elute IgG and was then processed ... | US |
Nondividing human diploid fibroblasts maintained in medium containing 0.5% calf serum do not survive when exposed to low doses of UV (254 nm). The extent of killing is dose and strain dependent. DNA excision repair-proficient cells are more resistant than excision repair-deficient cells. Results of measurements of the ... | US |
In this paper we show, based on symmetry considerations, that structural information cannot be obtained from the linear infrared dichroism of the dioxy vibrations of the phosphate group of nucleic acids. Consequently, the discrepancies between the results of x-ray structure measurements and linear dichroism measurement... | US |
Agents that elevate intracellular concentrations of cAMP in cultured spleen cells can augment the in vitro 19s humoral immune response to SRBC. DBcAMP and 8BrcAMP were more effective than MIX, CT, PGE1, or ISO in producing the enhanced PFC response, when they were present only during an early stage of immune induction.... | US |
The potency of the calcium ionophore A23187 in inducing three activities of human leukocytes (histamine secretion from basophils, enzyme secretion from PMNs, and proliferation of lymphocytes) was markedly dependent on the solvent (DMSO versus ethanol versus aqueous buffer) used for its initial sonication. While 0.1 mic... | US |
The influence(s) of chronic injections of alpha-fetoprotein (AFP), derived from murine amniotic fluid, on the natural history of immunopathology and autoantibody production in New Zealand mice was studied and compared to analagous treatment with albumin, transferrin, or phosphate-buffered saline. Treatment of young New... | US |
The epidemiology of inguinal hernia was investigated in a community survey in a neighbourhood of western Jerusalem in 1969-71. The current prevalence rate, excluding operated hernias, was 18 per 100 men aged 25 and over, and the lifetime prevalence, including operated hernias, was 24 per 100. Prevalence rose markedly w... | US |
Fetal erythropoiesis occurs during chronic bone marrow failure, or during recovery from marrow suppression. Fetal erythrocytes have HB F, with more G gamma than A gamma chains, "i" antigen, large MCV, characteristic enzyme levels, low carbonic anhydrase, low HB A2, and short life span. Many of these fetal characteristi... | US |
The beta subunit of TSH (TSH-beta) usually cannot be detected (less than 0.2 ng/ml) in the serum of normal individuals, whereas patients with primary hypothyroidism exhibit elevated TSH-beta levels (0.2-9.3 ng/ml), which increase further after the administration of TRH. Two patients were found to have large TSH-beta as... | US |
Immunoglobulin G purified from the serum of patients with Graves' disease, stimulated the thyroid of man, calf, and guinea pig (cAMP accumulation as the end-point) in vitro, and the thyroid of the mouse in vivo (LATS bioassay). The stimulatory effect on the thyroids of all four species was removed by adsorption of the ... | US |
We investigated the role of 3 alpha-androstanediol (3 alpha-diol) in the development of benign prostatic hyperplasia (BPH) and the apparent equilibrium of enzymes which metabolize it in normal and hyperplastic prostatic tissue of humans. We determined the endogenous concentrations of 3 alpha-diol, androsterone, its 3 a... | US |
The nature of localized atrial activation during atrial fibrillation was characterized in 34 patients following open heart surgery. Bipolar atrial electrograms (AEG) recorded in each patient with atrial fibrillation exhibited a myriad of sizes, shapes, polarities, amplitudes, and beat-to-beat intervals. On the basis of... | US |
Clinical observations and immunological evidence are presented to support a diagnosis of late onset asthma due to exposure to Aspergillus niger in a 70-year-old man. Asthma occurred on only three occasions, each time approximately 6 hr after exposure to an area containing Aspergillus niger. His sputum culture contained... | US |
In vivo and in vitro experiments were performed to determine how phenethyl alcohol (PEA) inhibits phospholipid synthesis in Escherichia coli. This drug drastically reduced the rate of incorporation of sn-glycerol 3-phosphate into the phospholipids of an sn-glycerol 3-phosphate auxotroph. PEA also reduced the rate of fa... | US |
A series of structurally related ansamycins have been analyzed, in a new immobilized template assay, to determine the mechanism by which they inhibit a ribonucleic acid-directed deoxyribonucleic acid (DNA) polymerase from Moloney murine leukemia virus. By this assay, we can better correlate specific structures of these... | US |
The rate of appearance, in a population of mouse-human heterokaryons, of cells with intermixed mouse and human surface antigens may be used to estimate the rate of lateral diffusion of the antigens in a single cell. Most heterokaryons appear to restrict diffusion of their surface antigens. These restrictions are altere... | US |
Normal rat liver cells (BRL-1) that respond to isoproterenol (beta+2), prostaglandin E1 (PGE+1) and adenosine (Ado+) with a rise in adenosine 3':5'-monophosphate (cAMP) content have been hybridized with rat hepatoma cells (H35) which do not respond to any of these agonists (beta-2, PGE-1 and Ado-). Both the initial hyb... | US |
1. The total testicular content of RNA, DNA and protein was found to decrease sharply in hamsters with shortened photoperiod and in ground squirrels during the spring breeding season. 2. RNA and DNA per g testes were found to increase in both animals, while protein per g testes remained fairly stable. 3. Cell-free prot... | US |
1. The effect of molt cycle stage and beta-ecdysone on protein synthesis in the horsehoe crab, Limulus polyphemus, was examined. 2. A pronounced decline in protein specific radioactivity after incubation with 14C-leucine was noted in muscle, midgut gland and operculum from postmolt to intermolt to premolt and in gut an... | US |
1. The activities of lysozyme, acid and alkaline phosphatases, beta-glucuronidase, amylase, lipase, glutamate-oxalacetate transaminase, and glutamate-pyruvate transaminase in the whole hemolymph and 4000 g pellets and supernatants of Mya arenaria were determined. 2. All of these enzymes, except for amylase, occurred in... | US |
Neurotensin (NT), substance P (SP) and morphine sulfate (MS) elevate plasma prolactin and growth hormone levels in both normal or estrogen-progesterone pretreated male rats. By contrast, steroid priming is required for TRF to exhibit PRL-releasing activity. Naloxone, an opiate receptor blocker, reverses the stimulatory... | US |
Stimulation of dopamine receptors by apomorphine inhibits episodic LH release in ovariectomized rats. The present study was designed to examine further the role of dopamine in this process. Unrestrained, unanesthetized rats with indwelling right atrial cannulae were bled continuously (30 or 50 microliters of whole bloo... | US |
Sera from a number of rhesus monkeys showed low or undetectable levels of LH according to radioimmunoassays which employ radioiodinated rhesus LH and antisera against rhesus LH or hCG. These same sera, when assayed by a system utilizing radioiodinated ovine LH and a unique anti-ovine LH serum which cross-reacts with LH... | US |
The spatial response of the magnetoencephalogram (MEG) to sources in the brain's cortex is compared with that of the electroencephalogram (EEG). This is done using computer modeling of the head which is approximated by 4 concentric spherical regions that represent the brain and surrounding bone and tissue. Lead fields ... | US |
First-degree relatives of lung-cancer patients and of patients with chronic obstructive pulmonary disease had significantly higher age-sex-race-smoking-adjusted rates of impaired forced expiration than first-degree relatives of patients with non-pulmonary disease or community-derived comparison series (neighbourhood co... | US |
Thirty-two eyes in 28 patients with anterior chamber angle neovascularization were prospectively treated with panretinal photocoagulation. Of 17 eyes that had less than 270 degrees angle closure, with an intraocular pressure of less than 40 mm Hg prior to treatment, 16 showed complete disappearance of the angle neovasc... | US |
Neonatal splenic B cells which are responsive to thymus-dependent antigens (TD) are exquisitely susceptible to induction of tolerance (1,2). This state of tolerance is not mediated by suppressor T cells and is not a result of suboptimal macrophage function (1 and footnote one). In adult mice, induction of B-cell tolera... | US |
Specific antibody producing human cell lines were established by preselecting antigen binding B-lymphocytes and subsequently transforming ("immortalizing") them with Epstein-Barr virus (EBV). NNP-binding B-cells were isolated from the blood of three donors with high anti-NNP titers. EBV-transformation led to polyclonal... | US |
This communication describes a method to obtain enriched populations of T-cells, B-cells and macrophages. Spleen cells were initially fractionated on nylon wool columns. The nylon wool adherent fraction was removed by mechanical agitation and further separated on the basis of adherence to a coated-plastic surface in th... | US |
A 61-year-old man developed clinical lupus syndrome with positive antinuclear antibody, positive lupus erythematosus (LE) cell preparation, and diffuse proliferative glomerulonephritis following 26 months of procainamide therapy. He was treated sequentially with prednisone and azathioprine (2 weeks), decreasing doses o... | US |
More than 80% of the hexachlorophene added to a Bacillus subtilis culture binds to the cells. Complete growth inhibition requires 6 x 10(5) molecules bound per cell. In contrast, more than 99% decanoate remains in solution and 3.8 x 10(7) molecules bound per cell are needed to inhibit growth. Centrifugation and resuspe... | US |
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