triage_status large_stringclasses 28
values | pmid large_stringlengths 7 13 | abstract large_stringlengths 49 3.69k | citation large_stringlengths 16 84 | token_count int32 17 878 | label int8 0 1 |
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Curatable | PMID:16861909 | Sister chromatid separation requires two steps of proteolysis. Securin, the chaperon and inhibitor of separase, is destructed in anaphase after polyubiquitination, and resulting activated separase cleaves the cohesin subunit Scc1/Rad21. Fission yeast securin/Cut2 and separase/Cut1 that form the complex are essential fo... | Cell Cycle 2006 Aug;5(16):1831-9 | 309 | 1 |
Method or reagent | PMID:15576932 | An in situ technique for studying the chromatin binding of proteins in single fission yeast cells (Schizosaccharomyces pombe) is described. Cells are permeabilized by enzymatic digestion and extracted with a detergent-containing buffer. This procedure removes soluble proteins, but proteins that are bound to insoluble c... | Methods Mol Biol 2005;296:181-8 | 135 | 0 |
Curatable | PMID:22604726 | Cells promote polarized growth by activation of Rho-family protein Cdc42 at the cell membrane. We combined experiments and modeling to study bipolar growth initiation in fission yeast. Concentrations of a fluorescent marker for active Cdc42, Cdc42 protein, Cdc42-activator Scd1, and scaffold protein Scd2 exhibited antic... | Science 2012 Jul 13;337(6091):239-43 | 192 | 1 |
Curatable | PMID:14637153 | Rho GTPases regulate fundamental processes including cell morphology and migration in various organisms. Guanine nucleotide exchange factor (GEF) has a crucial role in activating small GTPase by exchange GDP for GTP. In fission yeast Schizosaccharomyces pombe, six members of the Rho small GTPase family were identified ... | Biochem Biophys Res Commun 2003 Dec 12;312(2):414-20 | 214 | 1 |
Wrong organism | PMID:14595109 | Three meiosis-specific chromosomal components in budding yeast, Mek1, Red1, and Hop1, are required for recombination, proper segregation of homologs, and the meiotic recombination checkpoint. Mek1 is a protein kinase. Mutations that increase the size of the ATP binding pocket of Mek1 (mek1-as1) sensitize the kinase to ... | Mol Biol Cell 2004 Jan;15(1):11-23 | 296 | 0 |
Curatable | PMID:967158 | The assignment of the known ade genes to steps in purine biosynthesis in Schizosaccharomyces pombe has been completed with the demonstration that an ade3 mutants lacks FGAR amidotransferase, ade1A mutants lack GAR synthetase and ade1B mutants lack AIR synthetase. A comparison of enzyme activity with map position for ad... | Mol Gen Genet 1976 Sep 23;147(3):271-82 | 335 | 1 |
Curatable | PMID:16278445 | The stress-activated protein kinase (SAPK) pathway plays a central role in coordinating gene expression in response to diverse environmental stress stimuli. We examined the role of this pathway in the translational response to stress in Schizosaccharomyces pombe. Exposing wild-type cells to osmotic stress (KCl) resulte... | Eukaryot Cell 2005 Nov;4(11):1785-93 | 326 | 1 |
Wrong organism | PMID:11259593 | During the early stages of budding, cell wall remodeling and polarized secretion are concentrated at the bud tip (apical growth). The CBK1 gene, encoding a putative serine/threonine protein kinase, was identified in a screen designed to isolate mutations that affect apical growth. Analysis of cbk1Delta cells reveals th... | Mol Cell Biol 2001 Apr;21(7):2449-62 | 467 | 0 |
Transposon related | PMID:34791222 | Transposable elements (TEs) comprise a large proportion of the eukaryote genomes. Yet it remains poorly understood how TEs influence the fitness of the hosts carrying them. Here, we empirically test the impact of TEs on the host fitness in the fission yeast Schizosaccharomyces pombe. We find that two families of TEs (T... | Genome Biol Evol 2021 11 05;13(11) | 281 | 0 |
Curatable | PMID:26205977 | It is generally believed that silent chromatin is condensed and transcriptionally active chromatin is decondensed. However, little is known about the relationship between the condensation levels and gene expression. Here we report the condensation levels of interphase chromatin in the fission yeast Schizosaccharomyces ... | Nat Commun 2015 Jul 24;6:7753 | 230 | 1 |
Curatable | PMID:8367300 | DNA polymerases alpha and delta are essential enzymes believed to play critical roles in initiation and replication of chromosome DNA. In this study, we show that the genes for Schizosaccharomyces pombe (S.pombe) DNA polymerase alpha and delta (pol alpha+ and pol delta+) are essential for cell viability. Disruption of ... | Nucleic Acids Res 1993 Aug 11;21(16):3821-8 | 286 | 1 |
Review or comment | PMID:17851138 | In response to DNA damage, cells activate checkpoints to delay cell cycle progression and allow time for completion of DNA repair before commitment to S-phase or mitosis. During G2, many proteins collaborate to activate Chk1, an effector protein kinase that ensures the mitotic cyclin-dependent kinase remains in an inac... | DNA Repair (Amst) 2008 Feb 01;7(2):136-40 | 170 | 0 |
DNA recombination related | PMID:11983175 | The ade6-M26 allele of Schizosaccharomyces pombe creates a well-defined meiotic recombination hot spot that requires a specific sequence, 5'-ATGACGT-3', and the Atf1*Pcr1 transcription factor for activity. We find that M26 stimulates the formation of meiosis-specific double-strand DNA breaks at multiple sites surroundi... | Mol Cell 2002 Apr;9(4):847-55 | 194 | 0 |
Curatable, low priority | PMID:762155 | Two different allele-specific suppressor mutants of the fission yeast Schizosaccharomyces pombe produce opal (UGA) suppressor tRNAs. This was shown by the use of a new in vitro assay for eukaryotic nonsense suppression: a wheat germ extract is programmed with rabbit globin mRNAs and the readthrough products are studied... | J Biol Chem 1979 Mar 10;254(5):1546-51 | 195 | 1 |
Other | PMID:16314302 | Protein-nucleic acid interactions exhibit varying degrees of specificity. Relatively high affinity, sequence-specific interactions, can be studied with structure determination, but lower affinity, non-specific interactions are also of biological importance. We report simulations that predict the population of nucleic a... | Nucleic Acids Res 2005;33(21):6694-9 | 183 | 0 |
Wrong organism | PMID:14500360 | Human and mouse paralogues of the evolutionarily conserved mammalian HRAD9 and Mrad9 cell cycle checkpoint control genes have been isolated and called HRAD9B and Mrad9B, respectively. HRAD9B encodes a protein that is 414 amino acids long and is 55% similar and 35% identical to the HRAD9 gene product. The Mrad9B protein... | Cancer Res 2003 Sep 01;63(17):5291-8 | 355 | 0 |
Curatable | PMID:18978356 | Cells contain multiple formin isoforms that drive the assembly of profilin-actin for diverse processes. Given that many organisms also contain several profilin isoforms, specific formin/profilin pairs might be matched to optimally stimulate actin polymerization. We utilized a combination of bulk actin polymerization an... | J Biol Chem 2009 Jan 02;284(1):673-684 | 440 | 1 |
Wrong organism | PMID:23026052 | RecQ helicases are well conserved proteins from bacteria to human and function in various DNA metabolism for maintenance of genome stability. Five RecQ helicases are found in humans, whereas only one RecQ helicase has been described in lower eukaryotes. However, recent studies predicted the presence of a second RecQ he... | Biochem Biophys Res Commun 2012 Oct 26;427(3):623-8 | 256 | 0 |
Curatable | PMID:9766521 | We have isolated a murine cDNA, Mrad9, that is orthologous to the fission yeast rad9+ and human HRAD9 genes. Mrad9 encodes a 389 amino acid long, 42,032 Dalton protein that is 27% identical and 56% similar to Rad9p, and 82% identical and 88% similar to HRAD9, at the amino acid level. Expression of the Mrad9 cDNA in Sch... | J Cell Physiol 1998 Nov;177(2):241-7 | 277 | 1 |
Curatable | PMID:17868468 | DNA polymerase gamma(Pol-gamma) has been shown to be essential for maintenance of the mitochondrial genome (mtDNA) in the petite-positive budding yeast Saccharomyces cerevisiae. Budding yeast cells lacking mitochondria exhibit a slow-growing or petite-colony phenotype. Petite strains fail to grow on non-fermentable car... | BMC Genomics 2007 Sep 15;8:323 | 344 | 1 |
Method or reagent | PMID:25409521 | Analysis of accumulation of repair and checkpoint proteins at repair sites in yeast nuclei has conventionally used chemical agents, ionizing radiation or induction of endonucleases to inflict localized damage. In addition to these methods, similar studies in mammalian cells have used laser irradiation, which has the ad... | PLoS One 2014;9(11):e113325 | 268 | 0 |
Curatable | PMID:18523008 | Two functions are proposed for the conserved family of UCS proteins: helping to fold myosin motor proteins and stimulating the motor function of folded myosins. We examined both functions in yeast. The fission yeast UCS protein (Rng3p) concentrates in nodes containing myosin-II (Myo2) and other proteins that condense i... | Proc Natl Acad Sci U S A 2008 Jun 10;105(23):8014-9 | 382 | 1 |
Curatable | PMID:24818994 | SUMO is a small post-translational modifier, that is attached to lysine residues in target proteins. It acts by altering protein-protein interactions, protein localisation and protein activity. SUMO chains can also act as substrates for ubiquitination, resulting in proteasome-mediated degradation of the target protein.... | PLoS One 2014;9(5):e94182 | 318 | 1 |
Wrong organism | PMID:12657463 | Rab GTPases are key regulators of vesicular traffic in eukaryotic cells. Here we sought a global characterization and description of the Plasmodium falciparum family of Rab GTPases. We used a combination of bioinformatic analyses, experimental testing of predictions, structure modelling and phylogenetics. These analyse... | Gene 2003 Mar 13;306:13-25 | 444 | 0 |
Curatable | PMID:29967291 | In Schizosaccharomyces pombe , transcripts derived from the pericentromeric dg and dh repeats promote heterochromatin formation via RNAi as well as an RNAi-independent mechanism involving the RNA polymerase II (RNAPII)-associated RNA-binding protein Seb1 and RNA processing activities. We show that Seb1 promotes lo... | Genes Dev 2018 07 01;32(13-14):953-964 | 185 | 1 |
Curatable | PMID:28667014 | Glycolysis and the pentose phosphate pathway both play a central role in the degradation of glucose in all domains of life. Another metabolic route that can facilitate glucose breakdown is the gluconate shunt. In this shunt glucose dehydrogenase and gluconate kinase catalyze the two-step conversion of glucose into the ... | J Biol Chem 2017 08 18;292(33):13823-13832 | 401 | 1 |
Curatable | PMID:20833892 | Calmodulin in Schizosaccharomyces pombe is encoded by the cam1(+) gene, which is indispensable for both vegetative growth and sporulation. Here, we report how Cam1 functions in spore formation. We found that Cam1 preferentially localized to the spindle pole body (SPB) during meiosis and sporulation. Formation of the fo... | Eukaryot Cell 2010 Dec;9(12):1925-35 | 277 | 1 |
Curatable | PMID:11380623 | Protein phosphatase 2A (PP2A) is a serine/threonine phosphatase distributed in eukaryotes from yeast to human, and plays pivotal roles in diverse cellular functions such as metabolism, cell cycle progression, gene expression and development. PP2A holoenzyme is a heterodimer of a catalytic subunit C and a regulatory sub... | Genes Cells 2001 May;6(5):455-73 | 405 | 1 |
Curatable | PMID:9046095 | The Schizosaccharomyces pombe gpil+ gene was cloned by complementation of the Saccharomyces cerevisiae gpil mutant, which has temperature-sensitive defects in growth and glycosyl phosphatidylinositol (GPI) membrane anchoring or protein, and which is defective in vitro in the first step in GPI anchor assembly, the forma... | Yeast 1997 Feb;13(2):139-50 | 290 | 1 |
Wrong organism | PMID:10025943 | Protein kinase CK2 is a ubiquitous Ser/Thr kinase which phosphorylates a large number of proteins including several transcription factors. Recombinant Xenopus laevis CK2 phosphorylates both recombinant Saccharomyces cerevisiae and Schizosaccharomyces pombe TATA binding protein (TBP). The phosphorylation of TBP by CK2 r... | FEBS Lett 1999 Jan 29;443(3):256-60 | 172 | 0 |
Wrong organism | PMID:14993234 | Assembly at the mother-bud neck of a filamentous collar containing five septins (Cdc3, Cdc10, Cdc11, Cdc12, and Shs1) is necessary for proper morphogenesis and cytokinesis. We show that Cdc10 and Cdc12 possess GTPase activity and appropriate mutations in conserved nucleotide-binding residues abrogate GTP binding and/or... | J Cell Biol 2004 Mar 01;164(5):701-15 | 292 | 0 |
Curatable | PMID:27723196 | Antisense RNA has emerged as a crucial regulator of opposite-strand protein-coding genes in the long noncoding RNA (lncRNA) category, but little is known about their dynamics and decay process in the context of a stress response. Antisense transcripts from the fission yeast fbp1 locus (fbp1-as) are expressed in glucose... | Genes Cells 2016 Dec;21(12):1276-1289 | 325 | 1 |
Curatable | PMID:1547790 | Conjugation in the fission yeast Schizosaccharomyces pombe is controlled by the action of mating pheromones. Here I describe the isolation and characterization of M-factor, the pheromone released by M-type cells. M-factor is a nanopeptide in which the carboxy-terminal cysteine residue is carboxy-methylated and S-alkyla... | EMBO J 1992 Mar;11(3):951-60 | 226 | 1 |
Cell composition or WT feature | PMID:34100714 | Intracellular density impacts the physical nature of the cytoplasm and can globally affect cellular processes, yet density regulation remains poorly understood. Here, using a new quantitative phase imaging method, we determined that dry-mass density in fission yeast is maintained in a narrow distribution and exhibits h... | Elife 2021 06 08;10 | 198 | 0 |
Method or reagent | PMID:27587785 | Schizosaccharomyces pombe cells are rod shaped, and they grow by tip elongation. Growth ceases during mitosis and cell division; therefore, the length of a septated cell is a direct measure of the timing of mitotic commitment, and the length of a wild-type cell is an indicator of its position in the cell cycle. A large... | Cold Spring Harb Protoc 2016 09 01;2016(9) | 222 | 0 |
Curatable | PMID:25066056 | Dolichol is an obligate carrier of glycans for N-linked protein glycosylation, O-mannosylation, and GPI anchor biosynthesis. cis-prenyltransferase (cis-PTase) is the first enzyme committed to the synthesis of dolichol. However, the proteins responsible for mammalian cis-PTase activity have not been delineated. Here we ... | Cell Metab 2014 Sep 02;20(3):448-57 | 256 | 1 |
Wrong organism | PMID:17575050 | Innate immunity against pathogen infection is an evolutionarily conserved process among multicellular organisms. Arabidopsis SNC1 encodes a Resistance protein that combines attributes of multiple mammalian pattern recognition receptors. Utilizing snc1 as an autoimmune model, we identified a discrete protein complex con... | Genes Dev 2007 Jun 15;21(12):1484-93 | 171 | 0 |
Wrong organism | PMID:9188863 | CDC25B2, a protein tyrosine phosphatase closely related to the putative CDC25B oncogene, was identified in a Burkitt lymphoma cDNA library. CDC25B2 differs from CDC25B by a 14 residue insertion and a 41 residue deletion, which are both located in the amino-terminal region of the protein, upstream of the catalytic domai... | Oncogene 1997 May 22;14(20):2485-95 | 303 | 0 |
Curatable | PMID:10233152 | Transcriptional induction of many stress-response genes is dependent on stress-induced nuclear accumulation of stress-activated protein kinases (SAPKs). In the fission yeast Schizosaccharomyces pombe, nuclear accumulation of the SAPK Spc1 (also known as StyI) requires activating phosphorylation catalyzed by the SAPK ki... | Mol Biol Cell 1999 May;10(5):1395-407 | 288 | 1 |
Not physically mapped | PMID:24186545 | We have isolated 150 benomyl resistant mutants of the fission yeast Schizosaccharomyces pombe. Seven of these mutants were found to be cold sensitive for mitosis. These mutants were the subject of physiological, cytological and genetical characterisation. Growth and division of the seven mutants were similar to the wil... | Curr Genet 1982 Dec;6(3):195-201 | 250 | 0 |
Wrong organism | PMID:15283699 | Spermidine/spermine-N1-acetyltransferase (SSAT1) is a short-lived polyamine catabolic enzyme inducible by polyamines and polyamine analogues. Induction of SSAT1 plays an important role in polyamine homoeostasis, since the N1-acetylated polyamines can be excreted or oxidized by acetylpolyamine oxidase. We have purified ... | Biochem J 2004 Nov 15;384(Pt 1):139-48 | 466 | 0 |
Curatable | PMID:9802209 | We have cloned and sequenced the Schizosaccharomyces pombe ade10 gene encoding 5-phosphoribosyl-4-carboxamide 5-aminoimidazole transformylase inosine monophosphate cyclohydrolase. The sequence has an uninterrupted open reading frame of 1755 nucleotides corresponding to 585 amino acid residues. The deduced amino acid se... | Yeast 1998 Oct;14(14):1307-10 | 156 | 1 |
Curatable | PMID:19001497 | Cytoplasmic microtubule nucleation in the fission yeast Schizosaccharomyces pombe involves the interacting proteins Mto1 and Mto2, which are thought to recruit the gamma-tubulin complex (gamma-TuC) to prospective microtubule organizing centres. Mto1 contains a short amino-terminal region (CM1) that is conserved in high... | J Cell Sci 2008 Dec 01;121(Pt 23):3971-80 | 378 | 1 |
Curatable | PMID:11486016 | Cdc2 kinase is a master regulator of cell cycle progression in the fission yeast Schizosaccharomyces pombe. Our data indicate that Cdc2 phosphorylates replication factor Orp2, a subunit of the origin recognition complex (ORC). Cdc2 phosphorylation of Orp2 appears to be one of multiple mechanisms by which Cdc2 prevents ... | Mol Cell Biol 2001 Sep;21(17):5767-77 | 297 | 1 |
Curatable | PMID:16799560 | By sequence-specific binding to 3' UUU-OH, the La protein shields precursor (pre)-RNAs from 3' end digestion and is required to protect defective pre-transfer RNAs from decay. Although La is comprised of a La motif and an RNA-recognition motif (RRM), a recent structure indicates that the RRM beta-sheet surface is not i... | Nat Struct Mol Biol 2006 Jul;13(7):611-8 | 222 | 1 |
Curatable | PMID:11683417 | Regular segregation of homologous chromosomes during meiotic divisions is essential for the generation of viable progeny. In recombination-proficient organisms, chromosome disjunction at meiosis I generally occurs by chiasma formation between the homologs (chiasmate meiosis). We have studied meiotic stages in living re... | J Cell Sci 2001 Aug;114(Pt 15):2843-53 | 228 | 1 |
Curatable | PMID:28678660 | Despite its ubiquity in interphase eukaryotic nuclei, the functional significance of the RabI configuration, in which interphase centromeres are clustered at the nuclear envelope (NE) near the centrosome and telomeres localize at the opposite end of the nucleus, has remained mysterious. In a broad variety of organisms,... | Cell Cycle 2017 Aug 03;16(15):1392-1396 | 284 | 1 |
Wrong organism | PMID:12628935 | The mammalian homolog of the Schizosaccharomyces pombe Rad9 is involved in checkpoint signaling and the induction of apoptosis. While the mechanisms responsible for the regulation of human Rad9 (hRad9) are not known, hRad9 is subject to hyperphosphorylation in the response of cells to DNA damage. The present results de... | EMBO J 2003 Mar 17;22(6):1431-41 | 245 | 0 |
Curatable | PMID:16478984 | Stabilization and processing of stalled replication forks is critical for cell survival and genomic integrity. We characterize a novel DNA repair heterodimer of Nse5 and Nse6, which are nonessential nuclear proteins critical for chromosome segregation in fission yeast. The Nse5/6 dimer facilitates DNA repair as part of... | Mol Cell Biol 2006 Mar;26(5):1617-30 | 318 | 1 |
Curatable | PMID:22024164 | Cdc7/Hsk1 is a conserved kinase required for initiation of DNA replication that potentially regulates timing and locations of replication origin firing. Here, we show that viability of fission yeast hsk1Δ cells can be restored by loss of mrc1, which is required for maintenance of replication fork integrity, by cds1Δ, o... | J Cell Biol 2011 Oct 31;195(3):387-401 | 244 | 1 |
Curatable | PMID:25825517 | Cell-cell fusion is essential for fertilization. For fusion of walled cells, the cell wall must be degraded at a precise location but maintained in surrounding regions to protect against lysis. In fission yeast cells, the formin Fus1, which nucleates linear actin filaments, is essential for this process. In this paper,... | J Cell Biol 2015 Mar 30;208(7):897-911 | 217 | 1 |
Curatable | PMID:11577719 | Conditions for extracellular production of vitamin B6 compounds (B6), especially pyridoxal 5'-phosphate (PLP) by Schizosaccharomyces pombe leul strain were examined. The productivity was dependent on concentration of L-leucine in the culture medium: 30 mg/l gave the highest concentrations of total B6 and PLP. The viabl... | Biosci Biotechnol Biochem 2001 Aug;65(8):1789-95 | 205 | 1 |
Wrong organism | PMID:12200439 | Scc1/Mcd1 is a component of the cohesin complex that plays an essential role in sister chromatid cohesion in eukaryote cells. Knockout experiments of this gene have been described in budding yeast, fission yeast, and chicken cells, but no study has been reported on human Scc1 thus far. In this study, we found that an N... | J Biol Chem 2002 Nov 01;277(44):42306-14 | 275 | 0 |
Curatable | PMID:16024659 | Heterochromatin is critical for proper centromere and telomere function, and it plays a key role in the transcriptional silencing of specific genomic loci. In fission yeast, the Rik1 protein functions with the Clr4 histone methyltransferase at an early step in heterochromatin formation. Here, we use mass spectrometry a... | Genes Dev 2005 Jul 15;19(14):1705-14 | 247 | 1 |
Wrong organism | PMID:2209537 | Both genomic and full length cDNA clones of an Arabidopsis thaliana sugar carrier, STP1, have been obtained using a cDNA clone of the H+/hexose cotransporter from the green alga Chlorella kessleri as hybridization probe. The peptide predicted from these sequences in 522 amino acids long and has a molecular weight of 57... | EMBO J 1990 Oct;9(10):3045-50 | 318 | 0 |
Biotech | PMID:31494498 | Fifteen vitisin A-type pyranoanthocyanins (vAPs) were determined in bilberry wines fermented with Saccharomyces cerevisiae and Schizosaccharomyces pombe by HPLC-DAD and UPLC-DAD-ESI-MS/MS. The fermentation involving S. pombe enhanced the production of vAPs compared to the fermentation with pure S. cerevisiae. The forma... | Food Chem 2020 Feb 01;305:125438 | 269 | 0 |
Curatable | PMID:22024167 | We created two new mutants of fission yeast cofilin to investigate why cytokinesis in many organisms depends on this small actin-binding protein. These mutant cofilins bound actin monomers normally, but bound and severed ADP-actin filaments much slower than wild-type cofilin. Cells depending on mutant cofilins condense... | J Cell Biol 2011 Oct 31;195(3):485-98 | 220 | 1 |
Curatable | PMID:8423799 | Schizosaccharomyces pombe is becoming an increasingly useful organism for the study of cellular processes, since in certain respects, such as the cell cycle and splicing, it is similar to metazoans. Previous biochemical studies have shown that the DNA binding ability of S. pombe heat shock factor (HSF) is fully induced... | Mol Cell Biol 1993 Feb;13(2):749-61 | 348 | 1 |
Wrong organism | PMID:14769857 | In the development of hyphal germ tubes of Candida albicans, a band of septin forms at the base of the germ tube (basal septin band). Later, a septin ring forms, which organizes the first septum within the germ tube (septin ring). We have investigated the role of the Nim1 kinases, Gin4 and Hsl1, in the formation of the... | J Cell Biol 2004 Feb 16;164(4):581-91 | 242 | 0 |
Wrong organism | PMID:7916638 | We have isolated a cDNA clone from a Xenopus laevis tadpole cDNA library which probably codes for the large subunit of glutathione synthetase. The corresponding protein comprises 474 amino acids and shows a significant homology with the large subunit of glutathione synthetase of Schizosaccharomyces pombe. RNase protect... | Biochim Biophys Acta 1993 Sep 23;1174(3):295-8 | 120 | 0 |
Curatable | PMID:22065639 | Cdk1 controls many aspects of mitotic chromosome behavior and spindle microtubule (MT) dynamics to ensure accurate chromosome segregation. In this paper, we characterize a new kinetochore substrate of fission yeast Cdk1, Nsk1, which promotes proper kinetochore-MT (k-MT) interactions and chromosome movements in a phosph... | J Cell Biol 2011 Nov 14;195(4):583-93 | 268 | 1 |
Curatable | PMID:22992726 | Metabolic responses of unicellular organisms are mostly acute, transient, and cell-autonomous. Regulation of nutrient uptake in yeast is one such rapid response. High quality nitrogen sources such as NH(4)(+) inhibit uptake of poor nitrogen sources, such as amino acids. Both transcriptional and posttranscriptional mech... | J Biol Chem 2012 Nov 02;287(45):38158-67 | 396 | 1 |
Method or reagent | PMID:26305038 | Construction of multiply mutated strains for genetic interaction analysis and of strains carrying different epitope tags at multiple open reading frames for testing protein localization, abundance and protein-protein interactions is hampered by the availability of a sufficient number of different selectable markers. Mo... | Yeast 2015 Dec;32(12):703-10 | 366 | 0 |
Curatable | PMID:7502077 | The origin recognition complex (ORC), a multisubunit protein identified in Saccharomyces cerevisiae, binds to chromosomal replicators and is required for the initiation of cellular DNA replication. Complementary DNAs (cDNAs) encoding proteins related to the two largest subunits of ORC were cloned from various eukaryote... | Science 1995 Dec 08;270(5242):1667-71 | 249 | 1 |
Review or comment | PMID:32761854 | Research over the last two decades has identified a group of meiosis-specific proteins, consisting of budding yeast Spo13, fission yeast Moa1, mouse MEIKIN, and Drosophila Mtrm, with essential functions in meiotic chromosome segregation. These proteins, which we call meiosis I kinase regulators (MOKIRs), mediate two ma... | Bioessays 2020 10;42(10):e2000018 | 261 | 0 |
Curatable | PMID:10490657 | We have previously shown that replication of fission yeast chromosomes is initiated in distinct regions. Analyses of autonomous replicating sequences have suggested that regions required for replication are very different from those in budding yeast. Here, we present evidence that fission yeast replication origins are ... | Mol Cell Biol 1999 Oct;19(10):7228-36 | 336 | 1 |
Wrong organism | PMID:8804314 | The paired box transcription factor Pax-5 (B-cell-specific activator protein) is a key regulator of lineage-specific gene expression and differentiation in B-lymphocytes. We show that Pax-5 functions as a cell type-specific docking protein that facilitates binding of the early B-cell-specific mb-1 promoter by proteins ... | Genes Dev 1996 Sep 01;10(17):2198-211 | 303 | 0 |
Curatable | PMID:21873461 | The N(6)-(isopentenyl)adenosine (i(6)A) modification of some tRNAs at position A37 is found in all kingdoms and facilitates codon-specific mRNA decoding, but occurs in different subsets of tRNAs in different species. Here we examine yeasts' tRNA isopentenyltransferases (i.e., dimethylallyltransferase, DMATase, members ... | RNA 2011 Oct;17(10):1846-57 | 500 | 1 |
Method or reagent | PMID:28733418 | Schizosaccharomyces pombe cells initiate a sexual differentiation program, which comprises meiosis and spore formation, on nitrogen starvation. This protocol describes a simple procedure to induce meiosis and sporulation semisynchronously in heterozygous diploid S. pombe cells. The procedure is appropriate for a va... | Cold Spring Harb Protoc 2017 Sep 01;2017(9):pdb.prot091785 | 114 | 0 |
Wrong organism | PMID:14617801 | Mus81 is a highly conserved endonuclease with homology to the XPF subunit of the XPF-ERCC1 complex. In yeast Mus81 associates with a second subunit, Eme1 or Mms4, which is essential for endonuclease activity in vitro and for in vivo function. Human Mus81 binds to a homolog of fission yeast Eme1 in vitro and in vivo. We... | Mol Biol Cell 2004 02;15(2):552-62 | 262 | 0 |
Curatable | PMID:18667531 | The Smc5-Smc6 holocomplex plays essential but largely enigmatic roles in chromosome segregation, and facilitates DNA repair. The Smc5-Smc6 complex contains six conserved non-SMC subunits. One of these, Nse1, contains a RING-like motif that often confers ubiquitin E3 ligase activity. We have functionally characterized t... | Mol Biol Cell 2008 Oct;19(10):4099-109 | 331 | 1 |
Review or comment | PMID:17207637 | Yeast are eukaryotic unicellular organisms that are easy to cultivate and offer a wide spectrum of genetic and cytological tools for research. Yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe have successfully been used as models for human cell division cycle. Stress conditions, cellular ageing, failed mat... | Semin Cancer Biol 2007 Apr;17(2):112-21 | 142 | 0 |
Curatable | PMID:34918022 | Circular chromosomes have frequently been observed in tumors of mesenchymal origin. In the fission yeast Schizosaccharomyces pombe, deletion of pot1+ results in rapid telomere loss, and the resulting survivors have circular chromosomes. Fission yeast has 2 bromodomain and extra-terminal (BET) proteins, Bdf1 and Bdf2; b... | Biosci Biotechnol Biochem 2022 Jan 24;86(2):224-230 | 251 | 1 |
Curatable | PMID:28338873 | A contractile ring (CR) is involved in cytokinesis in animal and yeast cells. Although several types of actin-bundling proteins associate with F-actin in the CR, their individual roles in the CR have not yet been elucidated in detail. Ain1 is the sole α-actinin homologue in the fission yeast Schizosaccharomyces pombe a... | J Biochem 2017 Aug 01;162(2):93-102 | 305 | 1 |
Curatable | PMID:24656819 | The firing of eukaryotic origins of DNA replication requires CDK and DDK kinase activities. DDK, in particular, is involved in setting the temporal program of origin activation, a conserved feature of eukaryotes. Rif1, originally identified as a telomeric protein, was recently implicated in specifying replication timin... | Cell Rep 2014 Apr 10;7(1):53-61 | 225 | 1 |
Method or reagent | PMID:390094 | Recent improvements in the optics and electronics of flow cytometry systems, as well as in staining techniques, permit the assay of such minute cellular constituents as the DNA and protein contents of micro-organisms. To assess the usefulness of this technique, DNA and protein content distributions were determined in E... | J Gen Microbiol 1979 Aug;113(2):369-75 | 169 | 0 |
Cell composition or WT feature | PMID:23530189 | Statins are cholesterol-lowering drugs that inhibit 3-hydroxy-3-methyl-glutaryl-CoA (HMG-CoA) reductase, the rate-limiting enzyme in the synthesis of cholesterol via the mevalonate pathway. This pathway also produces coenzyme Q (a component of the respiratory chain), dolichols (important for protein glycosylation), and... | Proc Natl Acad Sci U S A 2013 Apr 09;110(15):5981-6 | 409 | 0 |
DNA recombination related | PMID:7498729 | The M26 mutation in the ade6 gene of Schizosaccharomyces pombe creates a hot spot of meiotic recombination. A single base substitution, the M26 mutation is situated within the open reading frame, near the 5' end. It has previously been shown that the heptanucleotide sequence 5' ATGACGT 3', which includes the M26 mutati... | Genetics 1995 Jun;140(2):469-78 | 358 | 0 |
Curatable | PMID:18753627 | The minichromosome maintenance (MCM) helicase, composed of subunits Mcm2-7, is essential for the initiation and elongation phases of DNA replication. Even when DNA synthesis is blocked, MCM continues DNA unwinding to some extent for activation of the replication checkpoint and then stops. However, the mechanism of regu... | Proc Natl Acad Sci U S A 2008 Sep 02;105(35):12973-8 | 285 | 1 |
Curatable | PMID:29416031 | Post-translational modifications of the transcription elongation complex provide mechanisms to fine-tune gene expression, yet their specific impacts on RNA polymerase II regulation remain difficult to ascertain. Here, in Schizosaccharomyces pombe, we examine the role of Cdk9, and related Mcs6/Cdk7 and Lsk1/Cdk12 kinase... | Nat Commun 2018 02 07;9(1):543 | 232 | 1 |
Curatable | PMID:24589736 | Kinesin-5 is a homotetrameric motor with its motor domain at the N-terminus. Kinesin-5 crosslinks microtubules and functions in separating spindle poles during mitosis. In this study, the motile properties of Cut7, fission yeast kinesin-5, were examined for the first time. In in vitro motility assays, full-length Cut7 ... | Biochem Biophys Res Commun 2014 Mar 28;446(1):231-4 | 186 | 1 |
Curatable | PMID:1538784 | Several mitotic and meiotic gene products are related to the microtubule motor kinesin, providing insight into the molecular basis of the complex motile events responsible for spindle formation and function. Of these genes, three have been shown to affect spindle structure when mutated. The most severe phenotype is see... | Nature 1992 Mar 05;356(6364):74-6 | 239 | 1 |
Wrong organism | PMID:22264161 | Although many techniques can be used to generate multitype-induced pluripotent stem (iPS) cells from multitype seed cells, improving the efficiency and shortening the period of cell reprogramming remain troublesome issues. In this study, to generate iPS cells, CD34⁺ cells, isolated from human amniotic fluid cells (HuAF... | Stem Cells Dev 2012 Aug 10;21(12):2322-32 | 359 | 0 |
Wrong organism | PMID:18682732 | In nature, yeasts are subject to predation by flies of the genus Drosophila. In response to nutritional starvation Saccharomyces cerevisiae differentiates into a dormant cell type, termed a spore, which is resistant to many types of environmental stress. The stress resistance of the spore is due primarily to a spore wa... | PLoS One 2008 Aug 06;3(8):e2873 | 187 | 0 |
Curatable | PMID:12604790 | Previous studies in Saccharomyces cerevisiae have defined an essential role for the Dbf4-Cdc7 kinase complex in the initiation of DNA replication presumably by phosphorylation of target proteins, such as the minichromosome maintenance (Mcm) complex. We have examined the phosphorylation of the Mcm complex by the Dfp1-Hs... | Proc Natl Acad Sci U S A 2003 Mar 04;100(5):2334-9 | 377 | 1 |
Wrong organism | PMID:9482730 | Polo-like kinases (Plks), named after the Drosophila gene product polo, have been implicated in the regulation of multiple aspects of mitotic progression, including the activation of the Cdc25 phosphatase, bipolar spindle formation and cytokinesis. Genetic analyses performed in yeast and Drosophila suggest a function f... | EMBO J 1998 Mar 02;17(5):1328-35 | 297 | 0 |
Transposon related | PMID:9774697 | The Tf2 retrotransposon, found in the fission yeast Schizosaccharomyces pombe, is nearly identical to its sister element, Tf1, in its reverse transcriptase-RNase H and integrase domains but is very divergent in the gag domain, the protease, the 5' untranslated region, and the U3 domain of the long terminal repeats. It ... | Mol Cell Biol 1998 Nov;18(11):6839-52 | 271 | 0 |
Curatable | PMID:15062096 | The halving of chromosome number that occurs during meiosis depends on three factors. First, homologs must pair and recombine. Second, sister centromeres must attach to microtubules that emanate from the same spindle pole, which ensures that homologous maternal and paternal pairs can be pulled in opposite directions (c... | Curr Biol 2004 Apr 06;14(7):560-72 | 376 | 1 |
Wrong organism | PMID:9278511 | In response to DNA damage, mammalian cells prevent cell cycle progression through the control of critical cell cycle regulators. A human gene was identified that encodes the protein Chk1, a homolog of the Schizosaccharomyces pombe Chk1 protein kinase, which is required for the DNA damage checkpoint. Human Chk1 protein ... | Science 1997 Sep 05;277(5331):1497-501 | 239 | 0 |
Wrong organism | PMID:10996242 | By using the rice EST database, we have isolated a 2.8 kb cDNA, termed Oryza sativa ORC1 (OsORC1), from rice (O. sativa) encoding a protein that shows homology with the eukaryotic ORC1 proteins. Alignment of the OsORC1 protein sequence with the sequence of ORC1 from human and yeasts S. cerevisiae and S. pombe showed a ... | Plant Sci 2000 Sep 08;158(1-2):33-39 | 311 | 0 |
Curatable | PMID:12181326 | Human damaged DNA-binding protein (DDB) is a heterodimer of p48/DDB2 and p127/DDB1 subunits. Mutations in DDB2 are responsible for Xeroderma Pigmentosum group E, but no mutants of mammalian DDB1 have been described. To study DDB1, the Schizosaccharomyces pombe DDB1 sequence homologue (ddb1(+)) was cloned, and a ddb1 de... | J Biol Chem 2002 Oct 25;277(43):41183-91 | 239 | 1 |
Database | PMID:10220000 | We have determined the complete nucleotide sequence of a 39,648 bp segment, contained in cosmid c32F12, derived from the right arm of chromosome II from the fission yeast Schizosaccharomyces pombe. Computer analysis of the sequence revealed the presence of 15 non-overlapping open reading-frames (ORFs) longer than 300 b... | Yeast 1999 Mar 30;15(5):419-26 | 259 | 0 |
Review or comment | PMID:34208016 | Long non-coding RNAs (lncRNAs) contribute to cell fate decisions by modulating genome expression and stability. In the fission yeast Schizosaccharomyces pombe , the transition from mitosis to meiosis results in a marked remodeling of gene expression profiles, which ultimately ensures gamete production and inheritance ... | Noncoding RNA 2021 Jun 11;7(2) | 177 | 0 |
Curatable | PMID:14735354 | The vacuolar H+-ATPases (V-ATPases) are ATP-dependent proton pumps responsible for acidification of intracellular compartments in eukaryotic cells. To investigate the functional roles of the V-ATPase in Schizosaccharomyces pombe, the gene vma1 encoding subunit A or vma3 encoding subunit c was disrupted. Both deletion m... | Mol Genet Genomics 2004 Mar;271(2):197-207 | 265 | 1 |
Other | PMID:29121868 | Identification of essential genes is not only useful for our understanding of the minimal gene set required for cellular life but also aids the identification of novel drug targets in pathogens. In this work, we present a simple and effective gene essentiality prediction method using information-theoretic features that... | BMC Bioinformatics 2017 Nov 09;18(1):473 | 282 | 0 |
Curatable | PMID:9447985 | In the fission yeast Schizosaccharomyces pombe, the onset of sexual development is controlled mainly by two external signals, nutrient starvation and mating pheromone availability. We have isolated a novel gene named rcd1+ as a key factor required for nitrogen starvation-induced sexual development. rcd1+ encodes a 283-... | Mol Cell Biol 1998 Feb;18(2):887-95 | 209 | 1 |
Curatable | PMID:14711417 | The spindle orientation checkpoint (SOC) in fission yeast has been proposed to delay metaphase-to-anaphase transition when the spindle poles are misaligned with respect to the long axis of the cell. This checkpoint is activated in the absence of either an actomyosin division ring or astral microtubules. Although the SO... | Curr Biol 2004 Jan 06;14(1):69-74 | 271 | 1 |
Wrong organism | PMID:10211818 | The reoccurrence of water molecules in crystal structures of RNase T1 was investigated. Five waters were found to be invariant in RNase T1 as well as in six other related fungal RNases. The structural, dynamical, and functional characteristics of one of these conserved hydration sites (WAT1) were analyzed by protein en... | Protein Sci 1999 Apr;8(4):722-30 | 286 | 0 |
Curatable | PMID:11279180 | The nascent pre-rRNA of eukaryotic ribosomes is fully transcribed and assembled into an 80-90 S nucleolar particle before being cleaved into mature ribosomal RNA. The interdependence of steps in the processing of this precursor RNA indicates that RNA processing, at least in part, acts as a quality control mechanism tha... | J Biol Chem 2001 May 18;276(20):16655-9 | 331 | 1 |
Wrong organism | PMID:7696344 | The polymerase chain reaction was used to amplify a cdc2 homologous sequence from Paramecium tetraurelia. The corresponding amino acid sequences exhibits about 50% similarity to the cdc2 proteins of other species. The Paramecium cdc2 encoded protein is 11 amino acids longer than S. pombe p34cdc2. There is one amino aci... | Biochim Biophys Acta 1995 Mar 16;1265(2-3):161-7 | 154 | 0 |
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