HTW-KI-Werkstatt/RamanSpectraEthanolicYeastFermentations

Dataset Card for Raman and NMR Spectra from Continuous Ethanolic Fermentation of Immobilized Yeast

Dataset Details

Dataset Description

This dataset contains Raman spectra acquired during the continuous ethanolic fermentation of sucrose using Saccharomyces cerevisiae (Baker's yeast). To facilitate continuous processing and high-quality optical measurements, the yeast cells were immobilized in calcium alginate beads.

The data covers process monitoring from two distinct experimental setups:

• One-Stage Fermentation: Performed in a 3 L bioreactor over approximately 15 hours (900 minutes) to monitor steady-state and dynamic transitions.
• Two-Stage Fermentation: Performed in a 2 L bioreactor over 50 hours (3000 minutes), focusing on the response to varying sucrose feed concentrations.

Measurements were taken online via a bypass flow-cell system. Raman spectra (785 nm excitation) provide vibrational data on sugars and ethanol. This dataset is intended for use in chemometrics, soft-sensor development, and real-time bioprocess control applications.

More details can be found in the paper: https://doi.org/10.1002%2Fbit.27112

Annotations

The spectra contain the following four annotations:

• Glucose [mol / L]
• Fructose [mol / L]
• Glycerol [mol / L]
• Ethanol [mol / L]

Citation

Please cite our paper, if you use the dataset: https://doi.org/10.1002%2Fbit.27112

BibTeX:

@article{legner2019application,
  title={Application of green analytical chemistry to a green chemistry process: Magnetic resonance and Raman spectroscopic process monitoring of continuous ethanolic fermentation},
  author={Legner, Robin and Wirtz, Alexander and Koza, Tim and Tetzlaff, Till and Nickisch-Hartfiel, Anna and Jaeger, Martin},
  journal={Biotechnology and bioengineering},
  volume={116},
  number={11},
  pages={2874--2883},
  year={2019},
  publisher={Wiley Online Library}
}