README.md

metadata

license: cc-by-4.0
task_categories:
  - image-to-image
tags:
  - microscopy
  - focus-stacking
  - image-fusion
  - computational-imaging
  - computer-vision
  - anonymous-review
pretty_name: Anonymous Microscopy Focus Test Set
size_categories:
  - n<1K
viewer: false

Anonymous Microscopy Focus Test Set

This repository contains an anonymized microscopy image test set prepared for double-blind peer review of the associated anonymous submission.

The dataset includes microscopy focus-stack TIFF sequences with fused reference images where available, plus additional JPEG microscopy image series. File and directory names have been replaced with neutral sample identifiers.

Directory Structure

data/
  sample_001/
    frame_000.tif
    frame_001.tif
    fusion.tif
  sample_002/
    ...
metadata.csv
manifest.csv
checksums_sha256.txt
ANONYMIZATION.md
LICENSE

Data Fields

metadata.csv gives sample-level information:

• sample_id: anonymized sample identifier.
• specimen_group: coarse specimen category.
• region: coarse anatomical/imaging region.
• modality: focus_stack_tiff or jpeg_series.
• magnification: reported only when needed for reproducibility and not identifying.
• z_step_um: reported only when needed for reproducibility and not identifying.
• num_files: number of files in the anonymized sample directory.
• has_fusion_reference: whether fusion.tif is provided.

manifest.csv gives file-level information including relative path, role, frame index, image size, byte size, and SHA-256 checksum.

Selected-Pair Protocol

The microscopy samples originate from focal stacks. For reproducible dual-focus preview evaluation, eligible stacks are converted into fixed two-plane inputs before any fusion method is run.

For a stack with N focal planes, the default selected planes lie near 0.3N and 0.7N. The selected planes must be separated by at least two focal-plane positions. If this condition is not met, the positions are adjusted toward the stack ends. For N=3, the protocol selects the first and third focal planes.

This package contains 20 anonymized samples. Eighteen samples satisfy N>=3 and are listed in selected_pairs.csv. The two N=2 samples remain in the dataset but are not included in the main selected-pair mean statistics.

Review-Period Anonymity

This dataset is provided in anonymized form for peer review. The public record, formal citation, author attribution, and any additional provenance details will be added after the review process permits deanonymization.

License

The image data are released under CC BY 4.0. During anonymous review, attribution information is intentionally withheld; please cite the associated paper after acceptance when bibliographic information becomes available.