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Animals require molecular signals to determine when to divert resources from somatic functions to reproduction . This decision is vital in animals that reproduce in an all-or-nothing mode , such as bristle worms: females committed to reproduction spend roughly half their body mass for yolk and egg production; following...
All organisms need energy to survive and grow . However , sources of energy are limited and so organisms need to decide how to spend the resources they have available . For instance , animals must choose whether they should continue to grow or if they should invest energy into reproduction instead . This decision becom...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "evolutionary", "biology", "biochemistry", "and", "chemical", "biology" ]
2016
Discovery of methylfarnesoate as the annelid brain hormone reveals an ancient role of sesquiterpenoids in reproduction
The structure of fitness landscapes is critical for understanding adaptive protein evolution . Previous empirical studies on fitness landscapes were confined to either the neighborhood around the wild type sequence , involving mostly single and double mutants , or a combinatorially complete subgraph involving only two ...
Proteins can evolve over time by changing their component parts , which are called amino acids . These changes usually happen one at a time and natural selection tends to preserve those changes that make the protein more efficient at its specific tasks , while discarding those that impair the protein’s activity . Howev...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "evolutionary", "biology" ]
2016
Adaptation in protein fitness landscapes is facilitated by indirect paths
Shoot branching is a primary contributor to plant architecture , evolving independently in flowering plant sporophytes and moss gametophytes . Mechanistic understanding of branching is largely limited to flowering plants such as Arabidopsis , which have a recent evolutionary origin . We show that in gametophytic shoots...
Most land plants have shoots that form branches and plants can regulate when and where they grow these branches to best exploit their environment . Plants with flowers and the more ancient mosses both have branching shoots , but these two groups of plants evolved to grow in this way independently of each other . Most s...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "plant", "biology", "developmental", "biology" ]
2015
Three ancient hormonal cues co-ordinate shoot branching in a moss
The gastrointestinal tract is abundantly colonized by microbes , yet the translocation of oral species to the intestine is considered a rare aberrant event , and a hallmark of disease . By studying salivary and fecal microbial strain populations of 310 species in 470 individuals from five countries , we found that tran...
Trillions of bacteria and other microbes live in the human body . The mouth and the gut in particular , are microbial hot spots at either end of the digestive tract . Every day , humans swallow around 1 . 5 liters of saliva , along with millions of oral microbes . Scientists believe that more than 99% of these microbes...
[ "Abstract", "Introduction", "Results", "and", "discussion", "Materials", "and", "methods" ]
[ "computational", "and", "systems", "biology", "microbiology", "and", "infectious", "disease" ]
2019
Extensive transmission of microbes along the gastrointestinal tract
HGF/Met signaling has recently been associated with basal-type breast cancers , which are thought to originate from progenitor cells residing in the luminal compartment of the mammary epithelium . We found that ICAM-1 efficiently marks mammary luminal progenitors comprising hormone receptor-positive and receptor-negati...
Throughout the life of a female mammal , the mammary glands undergo different phases of development to prepare for , and adapt to , feeding offspring . Luminal cells line the inside of branch-like structures throughout the mammary gland and are responsible for producing milk . When the mammary gland grows , new luminal...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "stem", "cells", "and", "regenerative", "medicine" ]
2015
Paracrine Met signaling triggers epithelial–mesenchymal transition in mammary luminal progenitors, affecting their fate
Sleep affects brain activity globally , but many cortical sleep waves are spatially confined . Local rhythms serve cortical area-specific sleep needs and functions; however , mechanisms controlling locality are unclear . We identify the thalamic reticular nucleus ( TRN ) as a source for local , sensory-cortex-specific ...
Falling asleep affects our behavior immediately and profoundly . During sleep , large electrical waves appear across the brain in areas responsible for consciousness , sensation and movement . In the cortex – the outer layer of the brain – sleep waves arise from networks that connect to the thalamus , a deeper structur...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "neuroscience" ]
2018
Thalamic reticular control of local sleep in mouse sensory cortex
Remarkably little is known about associations between age at menarche and sexually transmitted infections , although girls with earlier menarche tend to have earlier sexual debut and school drop-out , so an association might be expected . In a population-based survey of >3000 women aged 15–30 in northern Malawi we show...
For many girls in sub-Saharan Africa their first menstrual cycle can mean an abrupt end to childhood because a girl’s first period is often taken as a signal that she is ‘ready’ for sex and marriage . Most girls in this region have their first menstrual cycle between the ages of 13 and 18 , with the median being around...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "epidemiology", "and", "global", "health", "medicine" ]
2014
Earlier menarche is associated with a higher prevalence of Herpes simplex type-2 (HSV-2) in young women in rural Malawi
Mouse GnT1IP-L , and membrane-bound GnT1IP-S ( MGAT4D ) expressed in cultured cells inhibit MGAT1 , the N-acetylglucosaminyltransferase that initiates the synthesis of hybrid and complex N-glycans . However , it is not known where in the secretory pathway GnT1IP-L inhibits MGAT1 , nor whether GnT1IP-L inhibits other N-...
Proteins are made up of chains of amino acids that fold into three-dimensional shapes and many are assembled in a cell compartment known as the endoplasmic reticulum . From here , these new proteins move to another compartment called the Golgi , where they may be further modified before they are transported to their fi...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "cell", "biology" ]
2015
GnT1IP-L specifically inhibits MGAT1 in the Golgi via its luminal domain
Social information allows the rapid dissemination of novel information among individuals . However , an individual’s ability to use information is likely to be dependent on phenotypic constraints operating at three successive steps: acquisition , application , and exploitation . We tested this novel framework by quanti...
Animals need information to make decisions , and a quick way to get this information is to watch what others are doing . Animals , like humans , have different social networks that they could acquire this kind of ‘social information’ from , yet we know little about which networks they actually use . In addition , once ...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion" ]
[ "ecology" ]
2016
Sequential phenotypic constraints on social information use in wild baboons
Subunit vaccines comprised of glycoprotein D ( gD-2 ) failed to prevent HSV-2 highlighting need for novel strategies . To test the hypothesis that deletion of gD-2 unmasks protective antigens , we evaluated the efficacy and safety of an HSV-2 virus deleted in gD-2 and complemented allowing a single round of replication...
Herpes simplex virus 2 ( or HSV-2 ) infects millions of people worldwide and is the leading cause of genital diseases . The virus initially infects skin cells , but then spreads to nerve cells where it persists for life . Often , the virus remains in a dormant state for long periods of time and does not cause any sympt...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "microbiology", "and", "infectious", "disease" ]
2015
Herpes simplex type 2 virus deleted in glycoprotein D protects against vaginal, skin and neural disease
Cell surface receptors are central to the cell's ability to generate coordinated responses to the multitude of biochemical and physical cues in the microenvironment . However , the mechanisms by which receptors enable this concerted cellular response remain unclear . To investigate the effect of cellular tension on cel...
Cells constantly encounter diverse physical and biological signals in their surroundings . Information contained in these signals is transmitted from the cell surface to the interior to trigger coordinated changes in the cell’s behavior . Physical signals include the forces generated by cells pulling on one another or ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "cell", "biology" ]
2015
Discrete spatial organization of TGFβ receptors couples receptor multimerization and signaling to cellular tension
Invasive fungal infections cause 1 . 6 million deaths annually , primarily in immunocompromised individuals . Mortality rates are as high as 90% due to limited treatments . The azole class antifungal , fluconazole , is widely available and has multi-species activity but only inhibits growth instead of killing fungal ce...
Individuals with weakened immune systems – such as recipients of organ transplants – can fall prey to illnesses caused by fungi that are harmless to most people . These infections are difficult to manage because few treatments exist to fight fungi , and many have severe side effects . Antifungal drugs usually slow the ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "microbiology", "and", "infectious", "disease", "genetics", "and", "genomics" ]
2020
Synergistic and antagonistic drug interactions in the treatment of systemic fungal infections
Viral pathogens are a major threat to rice production worldwide . Although RNA interference ( RNAi ) is known to mediate antiviral immunity in plant and animal models , the mechanism of antiviral RNAi in rice and other economically important crops is poorly understood . Here , we report that rice resistance to evolutio...
Rice is a major food crop , providing over a fifth of all calories consumed by people around the world . As such , it is important to find ways to prevent the diseases that affect rice plants . Many of the viruses that infect rice are transferred between plants by insects and many insects carry more than one virus at a...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "plant", "biology", "microbiology", "and", "infectious", "disease" ]
2015
Viral-inducible Argonaute18 confers broad-spectrum virus resistance in rice by sequestering a host microRNA
Autophagy is a fundamental adaptive response to amino acid starvation orchestrated by conserved gene products , the autophagy ( ATG ) proteins . However , the cellular cues that activate the function of ATG proteins during amino acid starvation are incompletely understood . Here we show that two related stress-responsi...
Cells keep themselves healthy by breaking down unneeded or damaged internal structures via a process called autophagy . This process also helps a cell to survive if it is starved of nutrients . For example , if a cell does not receive enough amino acids , it cannot make new proteins . Autophagy can break down existing ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "biochemistry", "and", "chemical", "biology", "cell", "biology" ]
2015
The stress-responsive kinases MAPKAPK2/MAPKAPK3 activate starvation-induced autophagy through Beclin 1 phosphorylation
Hybridization is increasingly being recognized as a common process in both animal and plant species . Negative epistatic interactions between genes from different parental genomes decrease the fitness of hybrids and can limit gene flow between species . However , little is known about the number and genome-wide distrib...
In nature , closely related species often interbreed to produce hybrids . However , hybrids are often less fertile or unable to compete with parent species , making them less likely to thrive in the wild . When the genomes of two different species are mixed , versions of genes that are meant to work together can become...
[ "Abstract", "Introduction", "Results", "Materials", "and", "methods" ]
[ "genetics", "and", "genomics" ]
2014
High-resolution mapping reveals hundreds of genetic incompatibilities in hybridizing fish species
Munc18-1 orchestrates SNARE complex assembly together with Munc13-1 to mediate neurotransmitter release . Munc18-1 binds to synaptobrevin , but the relevance of this interaction and its relation to Munc13 function are unclear . NMR experiments now show that Munc18-1 binds specifically and non-specifically to synaptobre...
Nerve cells communicate with other nerve cells by releasing small molecules called neurotransmitters . The neurotransmitters are first packaged inside bubble-like structures called vesicles , which fuse with the membrane of the nerve cell when it is stimulated . Once the vesicle and membrane have fused , the neurotrans...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and" ]
[ "structural", "biology", "and", "molecular", "biophysics", "neuroscience" ]
2017
Autoinhibition of Munc18-1 modulates synaptobrevin binding and helps to enable Munc13-dependent regulation of membrane fusion
Reward contingencies are dynamic: outcomes that were valued at one point may subsequently lose value . Action selection in the face of dynamic reward associations requires several cognitive processes: registering a change in value of the primary reinforcer , adjusting the value of secondary reinforcers to reflect the n...
Most of us have experienced feeling full after a main course , only to discover that we somehow still have room for dessert . Eating a particular foodstuff to the point of satiety makes that item temporarily less appealing . This is an example of reward devaluation . We typically respond to this phenomenon by adjusting...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "neuroscience" ]
2018
Mediodorsal thalamus is required for discrete phases of goal-directed behavior in macaques
Coordinated walking in vertebrates and multi-legged invertebrates such as Drosophila melanogaster requires a complex neural network coupled to sensory feedback . An understanding of this network will benefit from systems such as Drosophila that have the ability to genetically manipulate neural activities . However , th...
Most animals need to be able to move to survive . Animals without limbs , such as snakes , move by generating by wave-like contractions along their bodies , whereas limbed animals , such as vertebrates and arthropods , walk by coordinating the movements of multi-jointed arms and legs . Locomotion in limbed animals invo...
[ "Abstract", "Introduction", "Results", "Discussion", "Conclusions", "Materials", "and", "methods" ]
[ "neuroscience" ]
2013
Quantification of gait parameters in freely walking wild type and sensory deprived Drosophila melanogaster
Viral replicase recruitment and long-range RNA interactions are essential for RNA virus replication , yet the mechanism of their interplay remains elusive . Flaviviruses include numerous important human pathogens , e . g . , dengue virus ( DENV ) and Zika virus ( ZIKV ) . Here , we revealed a highly conserved , conform...
Flaviviruses include a large family of viruses that are harmful to human health , such as dengue virus , West Nile virus and Zika virus . Understanding the details of the life cycle of these viruses is important for better controlling and treating the diseases that they cause . The genetic information of flaviviruses i...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "biochemistry", "and", "chemical", "biology", "microbiology", "and", "infectious", "disease" ]
2016
Viral RNA switch mediates the dynamic control of flavivirus replicase recruitment by genome cyclization
Mitotic spindle orientation is used to generate cell fate diversity and drive proper tissue morphogenesis . A complex of NuMA and dynein/dynactin is required for robust spindle orientation in a number of cell types . Previous research proposed that cortical dynein/dynactin was sufficient to generate forces on astral mi...
Before a cell divides , it must duplicate its DNA so that each new cell receives a complete set of genetic material . A structure called the mitotic spindle helps to ensure each new cell gets the correct amount of DNA . Cells often precisely position their mitotic spindle during division , and this spindle orientation ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "developmental", "biology", "cell", "biology" ]
2016
NuMA-microtubule interactions are critical for spindle orientation and the morphogenesis of diverse epidermal structures
Understanding the consequences of ongoing biodiversity changes for ecosystems is a pressing challenge . Controlled biodiversity-ecosystem function experiments with random biodiversity loss scenarios have demonstrated that more diverse communities usually provide higher levels of ecosystem functioning . However , it is ...
Ecosystems are at their healthiest when they have a high level of biodiversity – that is , a wide variety of different species all living together . But human activity is changing the environment and putting ecosystems under pressure . One of the places this is most evident is in the communities of organisms responsibl...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "ecology" ]
2020
Biodiversity mediates the effects of stressors but not nutrients on litter decomposition
In addition to the hallmark muscle stiffness , patients with recessive myotonia congenita ( Becker disease ) experience debilitating bouts of transient weakness that remain poorly understood despite years of study . We performed intracellular recordings from muscle of both genetic and pharmacologic mouse models of Beck...
Myotonia is a neuromuscular condition that causes problems with the relaxation of muscles following voluntary movements . One type of myotonia is Becker disease , also called recessive myotonia congenita . This is a genetic condition that causes muscle stiffness as a result of involuntary muscle activity . Patients may...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "medicine", "neuroscience" ]
2021
The mechanism underlying transient weakness in myotonia congenita
Women live on average longer than men but have greater levels of late-life morbidity . We have uncovered a substantial sex difference in the pathology of the aging gut in Drosophila . The intestinal epithelium of the aging female undergoes major deterioration , driven by intestinal stem cell ( ISC ) division , while lo...
Women live longer than men , and many age-related diseases are more common in one sex than the other . In addition , some treatments that extend the healthy lifespan of laboratory animals are more effective in females than in males . These include dietary restrictions , where food or specific dietary constituants are k...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "developmental", "biology", "short", "report", "immunology", "and", "inflammation" ]
2016
Sex difference in pathology of the ageing gut mediates the greater response of female lifespan to dietary restriction
Multicellularity arose as a result of adaptive advantages conferred to complex cellular assemblies . The arrangement of cells within organs endows higher-order functionality through a structure-function relationship , though the organizational properties of these multicellular configurations remain poorly understood . ...
In plants and other multicellular organisms , cells work together in tissues and organs to achieve outcomes that they would not be able to accomplish on their own . For example , the cells that make up the stem of a plant hold the leaves , flowers and other organs in place , and provide a transport network that allows ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "plant", "biology", "computational", "and", "systems", "biology" ]
2017
Topological analysis of multicellular complexity in the plant hypocotyl
Viral infection can dramatically alter a cell’s transcriptome . However , these changes have mostly been studied by bulk measurements on many cells . Here we use single-cell mRNA sequencing to examine the transcriptional consequences of influenza virus infection . We find extremely wide cell-to-cell variation in the pr...
When viruses infect cells , they take over the cell’s machinery and use it to express their own genes . This process has mostly been studied by looking at the average outcome of infection when many viruses infect many cells . However , it is less clear what happens in individual cells . For example , does the virus tak...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "microbiology", "and", "infectious", "disease" ]
2018
Extreme heterogeneity of influenza virus infection in single cells
Local activation and long-range inhibition are mechanisms conserved in self-organizing systems leading to biological patterns . A number of them involve the production by the developing cell of an inhibitory morphogen , but how this cell becomes immune to self-inhibition is rather unknown . Under combined nitrogen star...
Cyanobacteria are the only bacteria on Earth able to draw their energy directly from the sun in the same way that plants do . In addition , some strains are able to ‘fix’ the nitrogen present in the atmosphere: they can extract this gas and transform it into nitrogen-based compounds necessary for life . However , both ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "microbiology", "and", "infectious", "disease" ]
2020
HetL, HetR and PatS form a reaction-diffusion system to control pattern formation in the cyanobacterium nostoc PCC 7120
The influenza A virus ( IAV ) genome consists of eight negative-sense viral RNA ( vRNA ) segments that are selectively assembled into progeny virus particles through RNA-RNA interactions . To explore putative intersegmental RNA-RNA relationships , we quantified similarity between phylogenetic trees comprising each vRNA...
The viruses responsible for influenza evolve rapidly during infection . Changes typically emerge in two key ways: through random mutations in the genetic sequence of the virus , or by reassortment . Reassortment can occur when two or more strains infect the same cell . Once in a cell , viral particles ‘open up’ to rele...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "evolutionary", "biology", "microbiology", "and", "infectious", "disease" ]
2021
Parallel evolution between genomic segments of seasonal human influenza viruses reveals RNA-RNA relationships
Population averaging due to paracrine communication can arbitrarily reduce cellular response variability . Yet , variability is ubiquitously observed , suggesting limits to paracrine averaging . It remains unclear whether and how biological systems may be affected by such limits of paracrine signaling . To address this...
The human body is made up of many different types of cell that are each specialized to perform particular roles . Although each cell type has the same set of genes , the level of activity ( or “expression” ) of these genes varies between each type . Additionally , gene expression in cells of the same type can vary due ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "computational", "and", "systems", "biology" ]
2015
Paracrine communication maximizes cellular response fidelity in wound signaling
A complex interplay of viral , host , and ecological factors shapes the spatio-temporal incidence and evolution of human influenza viruses . Although considerable attention has been paid to influenza A viruses , a lack of equivalent data means that an integrated evolutionary and epidemiological framework has until now ...
To develop new therapies against infections caused by a virus , it is important to understand the virus's history—where , when , and why it has caused disease and how it has changed over time . For example , new human strains of the influenza type A virus originate from strains that infect animals and rapidly can becom...
[ "Abstract", "Introduction", "Results", "and", "discussion", "Materials", "and", "methods" ]
[ "evolutionary", "biology", "microbiology", "and", "infectious", "disease" ]
2015
The contrasting phylodynamics of human influenza B viruses
Cerebellar granule cells constitute the majority of neurons in the brain and are the primary conveyors of sensory and motor-related mossy fiber information to Purkinje cells . The functional capability of the cerebellum hinges on whether individual granule cells receive mossy fiber inputs from multiple precerebellar nu...
Learning a new motor skill , from riding a bicycle to eating with chopsticks , involves the cerebellum—a structure located at the base of the brain underneath the cerebral hemispheres . Although its name translates as ‘little brain' in Latin , the cerebellum contains more neurons than all other regions of the mammalian...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "neuroscience" ]
2013
Convergence of pontine and proprioceptive streams onto multimodal cerebellar granule cells
Planar cell polarity ( PCP ) regulates basal body ( BB ) docking and positioning during cilia formation , but the underlying mechanisms remain elusive . In this study , we investigate the uncharacterized gene Flattop ( Fltp ) that is transcriptionally activated during PCP acquisition in ciliated tissues . Fltp knock-ou...
Epithelial tissues are sheets of cells that line the surface of many parts of the body , including the airways and the inner ear . Small hair-like structures called cilia can be found on the top surface of many epithelial cells and are arranged in a precise , ordered pattern . Such patterning ensures that cilia can wor...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "developmental", "biology", "cell", "biology" ]
2014
Flattop regulates basal body docking and positioning in mono- and multiciliated cells
Cancer cells undergo lineage switching during natural progression and in response to therapy . NKX2-1 loss in human and murine lung adenocarcinoma leads to invasive mucinous adenocarcinoma ( IMA ) , a lung cancer subtype that exhibits gastric differentiation and harbors a distinct spectrum of driver oncogenes . In muri...
When cells become cancerous they grow uncontrollably and spread into surrounding healthy tissue . As the cancer progresses different genes are switched on and off which can cause tumor cells to change their identity and transition into other types of cell . How closely tumor cells resemble the healthy tissue they came ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "developmental", "biology", "cancer", "biology" ]
2021
An NKX2-1/ERK/WNT feedback loop modulates gastric identity and response to targeted therapy in lung adenocarcinoma
Extracellular adhesion molecules and their neuronal receptors guide the growth and branching of axons and dendrites . Growth cones are attracted to intermediate targets , but they must switch their response upon arrival so that they can move away and complete the next stage of growth . Here , we show that KPC-1 , a C ....
Neurons are the principal cells in the nervous system that send and receive information . A vast network of neurons helps transmit information throughout the brain and body . The end of the neuron that receives messages forms branched structures called dendrites , the shapes of which determine the signals the neuron re...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "developmental", "biology", "neuroscience" ]
2016
Precise regulation of the guidance receptor DMA-1 by KPC-1/Furin instructs dendritic branching decisions
Elucidating the conformational heterogeneity of proteins is essential for understanding protein function and developing exogenous ligands . With the rapid development of experimental and computational methods , it is of great interest to integrate these approaches to illuminate the conformational landscapes of target p...
Our cells contain thousands of proteins that perform many different tasks . Such tasks often involve significant changes in the shape of a protein that allow it to interact with other proteins or ligands . Understanding these shape changes can be an essential step for predicting and manipulating how proteins work or de...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "biochemistry", "and", "chemical", "biology" ]
2019
The dynamic conformational landscape of the protein methyltransferase SETD8
The magnitude and duration of vertebrate viremia is a critical determinant of arbovirus transmission , geographic spread , and disease severity . We find that multiple alphaviruses , including chikungunya ( CHIKV ) , Ross River ( RRV ) , and o’nyong ‘nyong ( ONNV ) viruses , are cleared from the circulation of mice by ...
Viruses transmitted by blood-feeding insects , such as mosquitoes and ticks , cause serious human diseases . In recent years these viruses ( also known as arboviruses ) have re-emerged at an unprecedented scale , leading to global outbreaks of diseases such as Zika or chikungunya fever . The severity of these diseases ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "microbiology", "and", "infectious", "disease" ]
2019
Discrete viral E2 lysine residues and scavenger receptor MARCO are required for clearance of circulating alphaviruses
The matricellular protein SMOC ( Secreted Modular Calcium binding protein ) is conserved phylogenetically from vertebrates to arthropods . We showed previously that SMOC inhibits bone morphogenetic protein ( BMP ) signaling downstream of its receptor via activation of mitogen-activated protein kinase ( MAPK ) signaling...
During the development of an embryo , a group of proteins known as growth factors stimulate cells to divide and direct how organs and limbs form . One family of growth factors called bone morphogenetic proteins ( BMPs ) regulate the formation of bone and many other tissues in the embryo . BMPs are released from cells ,...
[ "Abstract", "Introduction", "Results", "and", "discussion", "Materials", "and", "methods" ]
[ "developmental", "biology" ]
2017
SMOC can act as both an antagonist and an expander of BMP signaling
The nonenzymatic replication of RNA is a potential transitional stage between the prebiotic chemistry of nucleotide synthesis and the canonical RNA world in which RNA enzymes ( ribozymes ) catalyze replication of the RNA genomes of primordial cells . However , the plausibility of nonenzymatic RNA replication is undercu...
Though defining what makes something “alive” has proved challenging , one crucial feature of living things is the ability to copy genetic information and pass it on to the next generation . Nowadays , enzymes called polymerases copy genetic information encoded within the DNA of living cells . However , when life on Ear...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "biochemistry", "and", "chemical", "biology" ]
2016
Nonenzymatic copying of RNA templates containing all four letters is catalyzed by activated oligonucleotides
The virus SARS-CoV-2 can exploit biological vulnerabilities ( e . g . host proteins ) in susceptible hosts that predispose to the development of severe COVID-19 . To identify host proteins that may contribute to the risk of severe COVID-19 , we undertook proteome-wide genetic colocalisation tests , and polygenic ( pan ...
Individuals who become infected with the virus that causes COVID-19 can experience a wide variety of symptoms . These can range from no symptoms or minor symptoms to severe illness and death . Key demographic factors , such as age , gender and race , are known to affect how susceptible an individual is to infection . H...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion" ]
[ "epidemiology", "and", "global", "health", "genetics", "and", "genomics" ]
2021
A proteome-wide genetic investigation identifies several SARS-CoV-2-exploited host targets of clinical relevance
Niemann-Pick type C is a storage disease caused by dysfunction of NPC proteins , which transport cholesterol from the lumen of lysosomes to the limiting membrane of that compartment . Using freeze fracture electron microscopy , we show here that the yeast NPC orthologs , Ncr1p and Npc2p , are essential for formation an...
Niemann-Pick disease type C is a human disease that is characterized by severe neurological symptoms . The brains of children with this disease develop more slowly and adult patients have difficulty walking , coordinating movements , speaking clearly and they develop dementia . The disease is caused by mutations in two...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "cell", "biology" ]
2017
Niemann-Pick type C proteins promote microautophagy by expanding raft-like membrane domains in the yeast vacuole
The COVID-19 pandemic originating in the Wuhan province of China in late 2019 has impacted global health , causing increased mortality among elderly patients and individuals with comorbid conditions . During the passage of the virus through affected populations , it has undergone mutations , some of which have recently...
SARS-CoV-2 , the virus responsible for COVID-19 , has a genome made of RNA ( a nucleic acid similar to DNA ) that can mutate , potentially making the disease more transmissible , and more lethal . Most countries have monitored the rise of mutated strains using a technique called next generation sequencing ( NGS ) , whi...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion" ]
[ "epidemiology", "and", "global", "health", "tools", "and", "resources" ]
2021
FnCas9-based CRISPR diagnostic for rapid and accurate detection of major SARS-CoV-2 variants on a paper strip
Regulation of food intake is fundamental to energy homeostasis in animals . The contribution of non-nutritive and metabolic signals in regulating feeding is unclear . Here we show that enteric neurons play a major role in regulating feeding through specialized mechanosensory ion channels in Drosophila . Modulating acti...
Around one third of children and two thirds of adults in the US are thought to be overweight or obese . By increasing the risk of disorders such as heart disease , stroke , diabetes and some types of cancer , obesity has become one of the leading causes of preventable death worldwide and accounts for an increasing prop...
[ "Abstract", "Introduction", "Results", "and", "discussion", "Materials", "and", "methods" ]
[ "short", "report", "neuroscience" ]
2014
Regulation of food intake by mechanosensory ion channels in enteric neurons
Antibodies are critical components of adaptive immunity , binding with high affinity to pathogenic epitopes . Antibodies undergo rigorous selection to achieve this high affinity , yet some maintain an additional basal level of low affinity , broad reactivity to diverse epitopes , a phenomenon termed ‘polyreactivity’ . ...
To defend itself against bacteria and viruses , the body depends on a group of proteins known as antibodies . Each subset of antibodies undergoes a rigorous training regimen to ensure it recognizes a single epitope well – that is , one specific region on the surface of foreign , harmful organisms . Most antibodies stic...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "structural", "biology", "and", "molecular", "biophysics", "immunology", "and", "inflammation" ]
2020
Biochemical patterns of antibody polyreactivity revealed through a bioinformatics-based analysis of CDR loops
RNA polymerase II ( PolII ) transcribes RNA within a chromatin context , with nucleosomes acting as barriers to transcription . Despite these barriers , transcription through chromatin in vivo is highly efficient , suggesting the existence of factors that overcome this obstacle . To increase the resolution obtained by ...
DNA is tightly packaged in a material called chromatin inside the cell nucleus . To produce proteins this DNA must first be transcribed to produce a molecule of messenger RNA , which is then translated to make a protein . To assist with this process cells ‘unpack’ certain regions of the DNA so that enzymes that catalyz...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods", "Accession", "numbers" ]
[ "chromosomes", "and", "gene", "expression" ]
2014
The nucleosomal barrier to promoter escape by RNA polymerase II is overcome by the chromatin remodeler Chd1
Gagliano et al . ( Learning by association in plants , 2016 ) reported associative learning in pea plants . Associative learning has long been considered a behavior performed only by animals , making this claim particularly newsworthy and interesting . In the experiment , plants were trained in Y-shaped mazes for 3 day...
Associative learning is a simple learning ability found in most animals , which involves linking together two different cues . For example , the dogs in Pavlov’s famous experiment were trained to associate sound with the arrival of food , and eventually started salivating upon hearing the sound alone . Plants , like an...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "plant", "biology", "short", "report" ]
2020
Lack of evidence for associative learning in pea plants
Activity in prefrontal cortex ( PFC ) has been richly described using economic models of choice . Yet such descriptions fail to capture the dynamics of decision formation . Describing dynamic neural processes has proven challenging due to the problem of indexing the internal state of PFC and its trial-by-trial variatio...
In 1848 , a railroad worker named Phineas Gage suffered an accident that was to secure him a place in neuroscience lore . While constructing a new railway line , a mistimed explosion propelled an iron bar into the base of his skull , where it passed behind his left eye before exiting through the top of his head . Gage ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "neuroscience" ]
2015
Capturing the temporal evolution of choice across prefrontal cortex
Medical imaging can visualize characteristics of human cancer noninvasively . Radiomics is an emerging field that translates these medical images into quantitative data to enable phenotypic profiling of tumors . While radiomics has been associated with several clinical endpoints , the complex relationships of radiomics...
Medical imaging covers a wide range of techniques that are used to look inside the body , including X-rays , MRI scans and ultrasound . A process called radiomics uses computer algorithms to process the data collected by these techniques to identify and precisely measure a large number of features that would not otherw...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "computational", "and", "systems", "biology", "cancer", "biology" ]
2017
Defining the biological basis of radiomic phenotypes in lung cancer