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NucMM-Z Dataset

Overview

NucMM-Z (Neuronal Nuclei from Zebrafish) is a 3D electron microscopy (EM) dataset for nuclei instance segmentation from zebrafish brain tissue.

Property Value
Modality Electron Microscopy (EM)
Task Nuclei instance segmentation
Anatomy Zebrafish brain
Volume Size 64 × 64 × 64 voxels per patch
Train Volumes 27
Val Volumes 27
Total Size ~1.09 GB

Dataset Structure

NucMM-Z/
├── image.tif           # Full raw volume (~1 GB)
├── mask.h5             # Full annotation volume
├── README.txt          # Original readme
├── Image/
│   ├── train/          # 27 training patches (.h5)
│   └── val/            # 27 validation patches (.h5)
└── Label/
    ├── train/          # 27 training labels (.h5)
    └── val/            # 27 validation labels (.h5)

Label Format

  • Instance Segmentation: Each nucleus has a unique integer ID
  • Background: 0
  • Typical density: 50-300 nuclei per 64×64×64 volume

Usage with EasyMedSeg

from dataloader import NucMMZImageDataset, NucMMZVideoDataset

# Image mode (2D slices) - Recommended
dataset = NucMMZImageDataset(split='train')
sample = dataset[0]  # Returns dict with 'image' and 'mask'

# Video mode (3D volumes as frame sequences)
dataset = NucMMZVideoDataset(split='train')
video = dataset[0]  # Returns dict with 'frames' and 'masks'

Benchmark Results (SAM2)

Mode Model Mean Dice Mean IoU
Image sam2_hiera_large 0.3438 0.2566
Video sam2_video_hiera_large 0.0631 0.0425

Recommendation: Use image mode for this dataset.

Source

License

CC BY 4.0

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