input stringlengths 104 4.06k | output stringlengths 4 6.09k |
|---|---|
### Instruction:
Answer this question truthfully
### Response:
The CDC recommends that priority for collection and real-time RT-PCR testing should be given to lower respiratory tract specimens. Lower respiratory specimen testing appears to be more sensitive in the detection of MERS-CoV, when compared to specimens from... | What are the laboratory results for a Middle East respiratory syndrome coronavirus infection? |
### Instruction:
Please summerize the given abstract to a title
### Response:
SARS-CoV-2 RNA detection and persistence in wastewater samples: An experimental network for COVID-19 environmental surveillance in Padua, Veneto Region (NE Italy)
### Response:
| Background Clinical detection of SARS-CoV-2 RNA in stools supports the idea of wastewater-based epidemiology (WBE) as a precious tool for COVID-19 environmental surveillance. Successful detection of SARS-CoV-2 RNA in untreated wastewaters has been reported in several countries. This study investigated the presence and ... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Performance of the Innova SARS-CoV-2 antigen rapid lateral flow test in the Liverpool asymptomatic testing pilot: population based cohort study
### Response:
| OBJECTIVE: To assess the performance of the SARS-CoV-2 antigen rapid lateral flow test (LFT) versus polymerase chain reaction testing in the asymptomatic general population attending testing centres. DESIGN: Observational cohort study. SETTING: Community LFT pilot at covid-19 testing sites in Liverpool, UK. PARTICIPANT... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Investigation of potential safety hazards during medical waste disposal in SARS-CoV-2 testing laboratory
### Response:
| This study aims to investigate the potential safety hazards and provide reference for improving the medical waste disposal procedure in SARS-CoV-2 testing laboratory. Our SARS-CoV-2 testing group detected the RNA residue on the surface of medical waste with Droplet Digital PCR, and held a meeting to discuss the risks i... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Validating and optimizing the method for molecular detection and quantification of SARS-CoV-2 in wastewater
### Response:
| Wastewater surveillance of SARS-CoV-2 has become a promising tool to estimate population-level changes in community infections and the prevalence of COVID-19 disease. Although many studies have reported the detection and quantification of SARS-CoV-2 in wastewater, remarkable variation remains in the methodology. In thi... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Sensitive quantitative detection of SARS-CoV-2 in clinical samples using digital warm-start CRISPR assay
### Response:
| Quantifying severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in clinical samples is crucial for early diagnosis and timely medical treatment of coronavirus disease 2019. Here, we describe a digital warm-start CRISPR (dWS-CRISPR) assay for sensitive quantitative detection of SARS-CoV-2 in clinical samples. T... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Uncertainties in estimating SARS-CoV-2 prevalence by wastewater-based epidemiology
### Response:
| Wastewater-based epidemiology (WBE) is a promising approach for estimating population-wide COVID-19 prevalence through detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in wastewater However, various methodological challenges associated with WBE would affect the accuracy of prevalence estima... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Correlation of SARS-CoV-2 nucleocapsid antigen and RNA concentrations in nasopharyngeal samples from children and adults using an ultrasensitive and quantitative antigen assay
### Response:
| Background: Diagnosis of COVID-19 by PCR offers high sensitivity, but the utility of detecting samples with high cycle threshold (Ct) values remains controversial. Currently available rapid diagnostic tests (RDTs) for SARS-CoV-2 nucleocapsid antigens (Ag) have sensitivity well below PCR. The correlation of Ag and RNA q... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Validation of the RT-LAMP assay in a large cohort of nasopharyngeal swab samples shows that it is a useful screening method for detecting SARS-CoV-2 and its VOC variants
### Response:
| The COVID-19 pandemic is challenging the global supply chain and equipment needed for mass testing with RT-qPCR, the gold standard for SARS-CoV-2 diagnosis. Here, we propose the RT-LAMP assay as an additional strategy for rapid virus diagnosis. However, its validation as a diagnostic method remains uncertain. In this w... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Detection of SARS-CoV-2 variants by Abbott molecular, antigen, and serological tests
### Response:
| BACKGROUND: : Viral diversity presents an ongoing challenge for diagnostic tests, which need to accurately detect all circulating variants. The Abbott Global Surveillance program monitors severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) variants and their impact on diagnostic test performance. OBJECTIVES: :... |
### Instruction:
Please summerize the given abstract to a title
### Response:
SARS-CoV-2 Viral RNA Is Detected in the Bone Marrow in Post-Mortem Samples Using RT-LAMP
### Response:
| Since the emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in late 2019, viral RNA has been detected in several different human tissues and organs. This study reports the detection of SARS-CoV-2 RNA in the bone marrow. Post-mortem samples were taken in a sterile manner during two forensic autop... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Patterns of SARS-CoV-2 testing preferences in a national cohort in the United States
### Response:
| In order to understand preferences about SARS-CoV-2 testing, we conducted a discrete choice experiment among 4793 participants in the Communities, Households, and SARS-CoV-2 Epidemiology (CHASING COVID) Cohort Study from July 30-September 8, 2020. We used latent class analysis to identify distinct patterns of preferenc... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Increased SARS-CoV-2 Testing Capacity with Pooled Saliva Samples
### Response:
| We analyzed feasibility of pooling saliva samples for severe acute respiratory syndrome coronavirus 2 testing and found that sensitivity decreased according to pool size: 5 samples/pool, 7.4% reduction; 10 samples/pool, 11.1%; and 20 samples/pool, 14.8%. When virus prevalence is >2.6%, pools of 5 require fewer tests; w... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Fast and real-time electrical transistor assay for quantifying SARS-CoV-2 neutralizing antibodies
### Response:
| Due to the SARS-CoV-2 pandemic renewed attention has been directed towards viral neutralization assays and neutralizing antibodies quantification, for vaccine pre-clinical trials and determining vaccine efficacy over time. The gold standard to assess antibody titer is the plaque reduction neutralization test, an end-po... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Impact of repeated nasal sampling on detection and quantification of SARS-CoV-2
### Response:
| The impact of repeated sample collection on COVID-19 test performance is unknown. The FDA and CDC currently recommend the primary collection of diagnostic samples to minimize the perceived risk of false-negative findings. We therefore evaluated the association between repeated sample collection and test performance amo... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Quantification of mRNA Expression Using Single-Molecule Nanopore Sensing
### Response:
| [Image: see text] RNA quantification methods are broadly used in life science research and in clinical diagnostics. Currently, real-time reverse transcription polymerase chain reaction (RT-qPCR) is the most common analytical tool for RNA quantification. However, in cases of rare transcripts or inhibiting contaminants i... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Time-evolution of SARS-CoV-2 in wastewater during the first pandemic wave of COVID-19 in the metropolitan area of Barcelona
### Response:
| Two large wastewater treatment plants (WWTP), covering around 2.7 M inhabitants, which represents around 85% of the metropolitan area of Barcelona, were sampled before, during and after the implementation of a complete lockdown. Five one-step RT-qPCR assays, targeting the polymerase (IP2 and IP4), the envelope E and th... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Evaluating recovery, cost, and throughput of different concentration methods for SARS-CoV-2 wastewater-based epidemiology
### Response:
| As the COVID-19 pandemic continues to affect communities across the globe, the need to contain the spread of the outbreaks is of paramount importance. Wastewater monitoring of the SARS-CoV-2 virus, the causative agent responsible for COVID-19, has emerged as a promising tool for health officials to anticipate outbreaks... |
### Instruction:
Please summerize the given abstract to a title
### Response:
[Recording of SARS-CoV-2 laboratory tests in Germany]./ Erfassung der Labortestungen auf SARS-CoV-2 in Deutschland
### Response:
| The collection of data on SARS-CoV2 tests is central to the assessment of the infection rate in the context of the COVID-19 pandemic. At the Robert Koch Institute (RKI), data collected from various laboratory data recording systems are consolidated. First, this article aims to exemplify significant aspects regarding t... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Evaluation of an Automated High-Throughput Liquid-Based RNA Extraction Platform on Pooled Nasopharyngeal or Saliva Specimens for SARS-CoV-2 RT-PCR
### Response:
| SARS-CoV-2 RT-PCR with pooled specimens has been implemented during the COVID-19 pandemic as a cost- and manpower-saving strategy for large-scale testing. However, there is a paucity of data on the efficiency of different nucleic acid extraction platforms on pooled specimens. This study compared a novel automated high-... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Exploring beyond the limit: how comparative stochastic performance affects retesting outcomes in six commercial SARS CoV-2 nucleic acid amplification tests
### Response:
| OBJECTIVES: : To examine the comparative stochasticity profile of six commercial SARS-CoV-2 nucleic acid amplification tests (NAATs) and how this may affect retesting paradigms. METHODS: : Commercial quality control (QC) material was serially diluted in viral transport media to create a panel covering 10 – 10,000 copie... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Does sampling saliva increase detection of SARS-CoV-2 by RT-PCR? Comparing saliva with oro-nasopharyngeal swabs
### Response:
| The gold standard method in the diagnosis of SARS-CoV-2 infection is the detection of viral RNA in the nasopharyngeal sample by RT-PCR. Recently, saliva samples have been suggested as an alternative sample. In the present study, we aimed to compare RT-PCR results in nasopharyngeal, oro-nasopharyngeal and saliva samples... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Real-time optical analysis of a colorimetric LAMP assay for SARS-CoV-2 in saliva with a handheld instrument improves accuracy compared to endpoint assessment
### Response:
| Controlling the course of the COVID-19 pandemic will require widespread deployment of consistent and accurate diagnostic testing of the novel coronavirus SARS-CoV-2. Ideally, tests should detect a minimum viral load, be minimally invasive, and provide a rapid and simple readout. Current FDA-approved RT-qPCR-based stand... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Evaluation of the test accuracy of a SARS-CoV-2 rapid antigen test in symptomatic community dwelling individuals in the Netherlands
### Response:
| BACKGROUND: SARS-CoV-2 real-time reverse transcriptase polymerase chain reaction (qRT-PCR) is well suited for the diagnosis of clinically ill patients requiring treatment. Application for community testing of symptomatic individuals for disease control purposes however raises challenges. SARS-CoV-2 rapid antigen tests ... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Two-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification.
### Response:
| Diagnosis of the ongoing SARS-CoV-2 pandemic is a priority for all countries across the globe. Currently, reverse transcription quantitative PCR (RT-qPCR) is the gold standard for SARS-CoV-2 diagnosis as no permanent solution is available. However effective this technique may be, research has emerged showing its limita... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Optical imaging spectroscopy for rapid, primary screening of SARS-CoV-2: a proof of concept
### Response:
| Effective testing is essential to control the coronavirus disease 2019 (COVID-19) transmission. Here we report a-proof-of-concept study on hyperspectral image analysis in the visible and near-infrared range for primary screening at the point-of-care of SARS-CoV-2. We apply spectral feature descriptors, partial least sq... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Nested pool testing strategy for the reliable identification of individuals infected with SARS-CoV-2
### Response:
| The progress of the SARS-CoV-2 pandemic requires the design of cost-effective testing programs at large scale. To this end, pooling multiple samples can provide a solution. Defining a cost-effective strategy requires the establishment of an efficient deconvolution and re-testing procedure that eventually allows the ide... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Immunoassay of SARS-CoV-2 nucleocapsid proteins using novel red emission-enhanced carbon dot-based silica spheres
### Response:
| It is imminent to develop a new type of rapid COVID-19 detection method with high sensitivity. Here, we used novel red emission-enhanced carbon dot (CD)-based silica (RCS) spheres as the signals of lateral flow immunochromatography (LFI) to ultrasensitively detect novel severe acute respiratory syndrome coronavirus 2 n... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Comparison of saliva and oro-nasopharyngeal swab sample in the molecular diagnosis of COVID-19.
### Response:
| BACKGROUND Healthcare personnel are at risk of becoming infected while taking upper and/or lower respiratory tract specimens. Therefore, there is a need for sampling methods that do not risk infecting them. In this study, we aimed to compare the saliva and Oro-Nasopharyngeal Swab (ONS) sampling methods. METHODS Patient... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Same-day SARS-CoV-2 antigen test screening in an indoor mass-gathering live music event: a randomised controlled trial
### Response:
| BACKGROUND: The banning of mass-gathering indoor events to prevent SARS-CoV-2 spread has had an important effect on local economies. Despite growing evidence on the suitability of antigen-detecting rapid diagnostic tests (Ag-RDT) for mass screening at the event entry, this strategy has not been assessed under controlle... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Visual naked-eye detection of SARS-CoV-2 RNA based on covalent organic framework capsules
### Response:
| The ongoing outbreak of coronavirus disease 2019 (COVID-19) caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has highlighted that new diagnosis technologies are crucial for controlling the spread of the disease. Especially in the resources-limit region, conveniently operated detection methods ... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Saliva as a Reliable Sample for COVID-19 Diagnosis in Paediatric Patients
### Response:
| Saliva has been described a less invasive and easy to handle sample, compared to nasopharyngeal swabs (NPS), in the diagnosis of COVID-19 in adults. Although the advantages of using saliva is still more evident in paediatric patients, little is now about its sensitivity in this group. The aim of this study was to compa... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Will the extraction of COVID-19 from wastewater help flatten the curve?
### Response:
| With the potentially fatal effect of COVID-19 and its devastating impact on economies worldwide, some environmental scientist has suggested the use of waste from household sewage to trace the movement of SARS-CoV-2, within a given country. However, this approach is not without challenges where developing countries lack... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Development of Moore Swab and Ultrafiltration Concentration and Detection Methods for Salmonella Typhi and Salmonella Paratyphi A in Wastewater and Application in Kolkata, India and Dhaka, Bangladesh
### Response:
| Enteric fever is a severe systemic infection caused by Salmonella enterica serovar Typhi (ST) and Salmonella enterica serovar Paratyphi A (SPA). Detection of ST and SPA in wastewater can be used as a surveillance strategy to determine burden of infection and identify priority areas for water, sanitation, and hygiene in... |
### Instruction:
Please summerize the given abstract to a title
### Response:
At-home self-collection of saliva, oropharyngeal swabs and dried blood spots for SARS-CoV-2 diagnosis and serology: Post-collection acceptability of specimen collection process and patient confidence in specimens
### Response:
| BACKGROUND: Options to increase the ease of testing for SARS-CoV-2 infection and immune response are needed. Self-collection of diagnostic specimens at home offers an avenue to allow people to test for SARS-CoV-2 infection or immune response without traveling to a clinic or laboratory. Before this study, survey respond... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Rapid High-Throughput Whole-Genome Sequencing of SARS-CoV-2 by Using One-Step Reverse Transcription-PCR Amplification with an Integrated Microfluidic System and Next-Generation Sequencing
### Response:
| The long-lasting global COVID-19 pandemic demands timely genomic investigation of SARS-CoV-2 viruses. Here, we report a simple and efficient workflow for whole-genome sequencing utilizing one-step reverse transcription-PCR (RT-PCR) amplification on a microfluidic platform, followed by MiSeq amplicon sequencing. The met... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Practical challenges to the clinical implementation of saliva for SARS-CoV-2 detection
### Response:
| Due to global shortages of flocked nasopharyngeal swabs and appropriate viral transport media during the COVID-19 pandemic, alternate diagnostic specimens for SARS-CoV-2 detection are sought. The accuracy and feasibility of saliva samples collected and transported without specialized collection devices or media were ev... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Rapid detection and tracking of Omicron variant of SARS-CoV-2 using CRISPR-Cas12a-based assay
### Response:
| BACKGROUND: The newly emerged SARS-CoV-2 variant of concern (VOC) Omicron is spreading quickly worldwide, which manifests an urgent need of simple and rapid assay to detect and diagnose Omicron infection and track its spread. METHODS: To design allele-specific CRISPR RNAs (crRNAs) targeting the signature mutations in t... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Variability in instructions for performance of nasopharyngeal swabs across Canada in the era of COVID-19 - what type of swab is actually being performed?
### Response:
| BACKGROUND: The primary method of surveillance for the presence of SARS-CoV-2 is with nasopharyngeal swabs. Given the significant demand for nasopharyngeal swabs, a large number of previously untrained and unfamiliar staff are now performing this test. It was noted that there was significant heterogeneity in instructio... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Detection of SARS-CoV-2 RNA and Antibodies in Diverse Samples: Protocol to Validate the Sufficiency of Provider-Observed, Home-Collected Blood, Saliva, and Oropharyngeal Samples
### Response:
| BACKGROUND: The response in the United States to the coronavirus disease (COVID-19) pandemic has been hampered by a lack of aggressive testing for the infection. Testing for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the cornerstone of an effective public health response. However, efforts to test h... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Detection and quantification of SARS-CoV-2 RNA in wastewater influent in relation to reported COVID-19 incidence in Finland
### Response:
| Wastewater-based surveillance is a cost-effective concept for monitoring COVID-19 pandemics at a population level. Here, SARS-CoV-2 RNA was monitored from a total of 693 wastewater (WW) influent samples from 28 wastewater treatment plants (WWTP, N = 21-42 samples per WWTP) in Finland from August 2020 to May 2021, cover... |
### Instruction:
Please summerize the given abstract to a title
### Response:
An optimized and robust PEG precipitation method for detection of SARS-CoV-2 in wastewater
### Response:
| Wastewater-based epidemiology is currently being utilized to monitor the dissemination of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), on a population scale. The detection of SARS-CoV-2 in wastewater is highly influenced by methodologies used for its isolation, concentration and RNA extraction. Alt... |
### Instruction:
Please summerize the given abstract to a title
### Response:
SARS-CoV-2 in hospital air as revealed by comprehensive respiratory viral panel sequencing
### Response:
| BACKGROUND: Nosocomially acquired severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) infection has become the most significant pandemic of our lifetime. Though its transmission was essentially attributed to droplets from an infected person, with recent advancements in knowledge, aerosol transmission seem... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Evaluation of Specimen Types and Saliva Stabilization Solutions for SARS-CoV-2 Testing
### Response:
| Identifying SARS-CoV-2 infections through aggressive diagnostic testing remains critical to tracking and curbing the spread of the COVID-19 pandemic. Collection of nasopharyngeal swabs (NPS), the preferred sample type for SARS-CoV-2 detection, has become difficult due to the dramatic increase in testing and consequent ... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Salivary SARS-CoV-2 antigen rapid detection: A prospective cohort study
### Response:
| BACKGROUND AND AIM: SARS-CoV-2 quick testing is relevant for the containment of new pandemic waves. Antigen testing in self-collected saliva might be useful. We compared salivary and naso-pharyngeal swab (NPS) SARS-CoV-2 antigen detection by a rapid chemiluminescent assay (CLEIA) and two different point-of-care (POC) i... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Diagnostic Performance of Ag-RDTs and NAAT for SARS-CoV2 Identification in Symptomatic Patients in Catalonia
### Response:
| The use of rapid antigenic tests (Ag-RDTs) to diagnose a SARS-CoV-2 infection has become a common practice recently This study aimed to evaluate performance of Abbott PanbioTM Ag-RDTs with regard to nucleic acid amplification testing (NAAT) in the early stages of the disease A cohort of 149,026 infected symptomatic pat... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Sports balls as potential SARS-CoV-2 transmission vectors
### Response:
| Objects passed from one player to another have not been assessed for their ability to transmit severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) We found that the surface of sport balls, notably a football, tennis ball, golf ball, and cricket ball could not harbour inactivated virus when it was swabbed onto ... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Field-deployable, rapid diagnostic testing of saliva for SARS-CoV-2
### Response:
| To safely re-open economies and prevent future outbreaks, rapid, frequent, point-of-need, SARS-CoV-2 diagnostic testing is necessary However, existing field-deployable COVID-19 testing methods require the use of uncomfortable swabs and trained providers in PPE, while saliva-based methods must be transported to high com... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Implementation study of SARS-CoV-2 antigen lateral flow tests in men's professional (Premiership) rugby union sports squads in England during the COVID-19 pandemic
### Response:
| This study evaluated the validity and utility of antigen-detection rapid diagnostic tests (Ag-RDTs) for SARS-CoV-2 in elite sports. The data on utility, ease of use and application for Ag-RDTs as a new testing format were positive from players and staff. This evaluation was limited by the low prevalence of SARS-CoV-2 c... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Capacity of existing wastewater treatment plants to treat SARS-CoV-2. A review
### Response:
| Water is one of many viral transmission routes, and the presence of Severe Acute Respiratory Syndrome Corona Virus 2 (SARS-CoV-2) in wastewater has brought attention to its treatment. SARS CoV-2 primarily transmits in the air but the persistence of the virus in the water possibly can serve as a secondary source even th... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Potential spreading risks and disinfection challenges of medical wastewater by the presence of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) viral RNA in septic tanks of Fangcang Hospital
### Response:
| The outbreak of coronavirus infectious disease-2019 (COVID-19) pneumonia raises the concerns of effective deactivation of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in medical wastewater by disinfectants. In this study, we evaluated the presence of SARS-CoV-2 viral RNA in septic tanks of Wuchang C... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Wastewater surveillance demonstrates high predictive value for COVID-19 infection on board repatriation flights to Australia
### Response:
| Controlling importation and transmission of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) from overseas travelers is essential for countries, such as Australia, New Zealand, and other island nations, that have adopted a suppression strategy to manage very low community transmission. Wastewater surveillan... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Large-Scale, In-House Production of Viral Transport Media To Support SARS-CoV-2 PCR Testing in a Multihospital Health Care Network during the COVID-19 Pandemic
### Response:
| The COVID-19 pandemic has severely disrupted worldwide supplies of viral transport media (VTM) due to widespread demand for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) reverse transcription-PCR (RT-PCR) testing. In response to this ongoing shortage, we began production of VTM in-house in support of dia... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Nasopharyngeal Specimen Collection from Suspected Covid-19 Cases, Making the Procedure Safe
### Response:
| Objective: To comparison was made between standard (control group) nasopharyngeal sample collection technique for RT-PCR and modified technique and the outcome was compared in terms of the proportion of positive results of Rt-PCR tests. Study Design: Double blinded randomized clinical trial. Place and Duration of Study... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Evaluation of seven commercial RT-PCR kits for COVID-19 testing in pooled clinical specimens
### Response:
| INTRODUCTION: There are more than 350 RT-PCR COVID-19 testing kits commercially available but these kits has not been evaluated for pooled sample testing. Thus, this study was planned to compare and evaluate seven commercially available kits for pooled samples testing. METHODS: Diagnostic accuracy of (1) TRUPCR SARS-Co... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Nested pool testing strategy for the diagnosis of infectious diseases
### Response:
| The progress of the SARS-CoV-2 pandemic requires the design of large-scale, cost-effective testing programs. Pooling samples provides a solution if the tests are sensitive enough. In this regard, the use of the gold standard, RT-qPCR, raises some concerns. Recently, droplet digital PCR (ddPCR) was shown to be 10–100 ti... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Comparison of saliva and oro-nasopharyngeal swab sample in the molecular diagnosis of COVID-19
### Response:
| SUMMARY BACKGROUND Healthcare personnel are at risk of becoming infected while taking upper and/or lower respiratory tract specimens Therefore, there is a need for sampling methods that do not risk infecting them In this study, we aimed to compare the saliva and Oro-Nasopharyngeal Swab (ONS) sampling methods METHODS Pa... |
### Instruction:
Please summerize the given abstract to a title
### Response:
SARS-CoV-2 RNA in urban wastewater samples to monitor the COVID-19 pandemic in Lombardy, Italy (March–June 2020)
### Response:
| Wastewater-based viral surveillance was proposed as a promising approach to monitor the circulation of SARS-CoV-2 in the general population. The aim of this study was to develop an analytical method to detect SARS-CoV-2 RNA in urban wastewater, and apply it to follow the trends of epidemic in the framework of a surveil... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Safe Management Strategies in Clinical Forensic Autopsies of Confirmed COVID-19 Cases
### Response:
| To date, there is poor evidence on the transmission of infection in individuals handling the bodies of deceased persons infected with SARS-CoV-2 and in particular, during autopsies. The aim of this study was to demonstrate that when appropriate strategies are adopted autopsy is a safe procedure with a minimal infection... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Evaluation of a chemiluminescent enzyme immunoassay-based high-throughput SARS-CoV-2 antigen assay for the diagnosis of COVID-19: The VITROS® SARS-CoV-2 Antigen Test
### Response:
| A high-throughput, fully automated antigen detection test for SARS-CoV-2 is a viable alternative to reverse-transcription polymerase chain reaction (RT-qPCR) for mass screening during outbreaks. In this study, we compared RT-qPCR for viral load and the VITROS® SARS-CoV-2 Antigen Test with reference to the results of th... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Evaluation of Sampling, Analysis, and Normalization Methods for SARS-CoV-2 Concentrations in Wastewater to Assess COVID-19 Burdens in Wisconsin Communities
### Response:
| Wastewater surveillance for SARS-CoV-2 provides an approach for assessing the infection burden across a sewer service area. For these data to be useful for public health, measurement variability and the relationship to case data need to be established. We determined SARS-CoV-2 RNA concentrations in the influent of 12 w... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Plasmon-enhanced Quantitative Lateral Flow Assay for Femtomolar Detection of SARS-CoV-2 Antibodies and Antigens
### Response:
| Lateral flow assays (LFAs) are the cornerstone of point-of-care diagnostics. Although rapid and inexpensive, they are 1000-fold less sensitive than laboratory-based tests and cannot be used for definitive negative diagnosis. Here, we overcome this fundamental limitation by employing plasmonically-enhanced nanoscale col... |
### Instruction:
Please summerize the given abstract to a title
### Response:
SARS-CoV-2 Subgenomic N (sgN) Transcripts in Oro-Nasopharyngeal Swabs Correlate with the Highest Viral Load, as Evaluated by Five Different Molecular Methods
### Response:
| The COVID-19 pandemic has forced diagnostic laboratories to focus on the early diagnostics of SARS-CoV-2. The positivity of a molecular test cannot respond to the question regarding the viral capability to replicate, spread, and give different clinical effects. Despite the fact that some targets are covered by commerci... |
### Instruction:
Please summerize the given abstract to a title
### Response:
[Evaluation of a Visually-Read Rapid Antigen Test Kit (SGA V-Chek) for Detection of SARS-CoV-2 Virus]./ SARS-CoV-2 Virüs Tespitinde Görsel Okunan Hizli Antijen Test Kitinin (SGA V-Chek) Degerlendirilmesi
### Response:
| Although the reverse transcriptase polymerase chain reaction (RT-PCR) method has been accepted as the reference method in the detection of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) RNA, it requires special laboratory conditions, complicated and expensive laboratory instruments, competent laboratory s... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Multiplex rt-Real Time PCR assays for diagnostic testing of SARS-CoV-2 and seasonal influenza viruses. A challenge of the phase 3 pandemic setting
### Response:
| BACKGROUND: Pandemic COVID-19 disease represents a challenge for health-care structures and the molecular confirmation of samples from infected individuals is crucial and therefore guides public health decision making. Clusters and possibly increased diffuse transmission could occur in the context of the next influenza... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Potentiometric Biosensors Based on Molecular-Imprinted Self-Assembled Monolayer Films for Rapid Detection of Influenza A Virus and SARS-CoV-2 Spike Protein
### Response:
| [Image: see text] Rapid, yet accurate and sensitive testing has been shown to be critical in the control of spreading pandemic diseases such as COVID-19. Current methods which are highly sensitive and can differentiate different strains are slow and cannot be conveniently applied at the point of care. Rapid tests, mean... |
### Instruction:
Please summerize the given abstract to a title
### Response:
SARS-CoV-2 RNA Extraction Using Magnetic Beads for Rapid Large-Scale Testing by RT-qPCR and RT-LAMP
### Response:
| Rapid large-scale testing is essential for controlling the ongoing pandemic of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The standard diagnostic pipeline for testing SARS-CoV-2 presence in patients with an ongoing infection is predominantly based on pharyngeal swabs, from which the viral RNA is extr... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Tracking SARS-CoV-2 RNA through the wastewater treatment process
### Response:
| The municipal sewage carries the new coronavirus (SARS-CoV-2), shed by COVID-19 patients, to wastewater treatment plants. Proper wastewater treatment can provide an important barrier for preventing uncontrolled discharged of the virus into the environment. However, the role of the different wastewater treatment stages ... |
### Instruction:
Please summerize the given abstract to a title
### Response:
In silico analysis of mismatches in RT-qPCR assays of 177 SARS-CoV-2 sequences from Brazil
### Response:
| INTRODUCTION: Quantitative reverse transcription polymerase chain reaction (RT-qPCR) can detect the severe acute respiratory syndrome Coronavirus-2 (SARS-CoV-2) in a highly specific manner. However, a decrease in the specificity of PCR assays for their targets may lead to false negative results. METHODS: Here, 177 high... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Comparison of Abbott COVID-19 testing Platforms for timely results- CMCVAMC experience
### Response:
| INTRODUCTION/OBJECTIVE: COVID-19 is a new disease, caused by the SARS-CoV-2 coronavirus capable of causing severe disease and death. The Alinity-m has a high throughput and random-access features that are not on the Abbott m2000, both of which had been validated and brought into clinical use for high throughput SARS-Co... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Swabbing the Urban Environment - A Pipeline for Sampling and Detection of SARS-CoV-2 From Environmental Reservoirs
### Response:
| To control community transmission of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) during the 2020 global pandemic, most countries implemented strategies based on direct human testing, face covering, and surface disinfection. Under the assumption that the main route of transmission includes aerosols and ... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Rapid point-of-care detection of SARS-CoV-2 using reverse transcription loop-mediated isothermal amplification (RT-LAMP)
### Response:
| BACKGROUND: Fast, reliable and easy to handle methods are required to facilitate urgently needed point-of-care testing (POCT) in the current coronavirus pandemic. Life-threatening severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has rapidly spread all over the world, infecting more than 33,500,000 people an... |
### Instruction:
Please summerize the given abstract to a title
### Response:
External Quality Assessment for Molecular Detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in Clinical Laboratories
### Response:
| Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) poses a huge threat to public health. Viral nucleic acid testing is the diagnostic gold standard and can play an important role in the prevention and control of this infection. In this study, bacteriophage MS2 virus-like particles encapsulating specific RNA s... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Low Volume in Vitro Diagnostic Proton NMR Spectroscopy of Human Blood Plasma for Lipoprotein and Metabolite Analysis: Application to SARS-CoV-2 Biomarkers
### Response:
| The utility of low sample volume in vitro diagnostic (IVDr) proton nuclear magnetic resonance (1H NMR) spectroscopic experiments on blood plasma for information recovery from limited availability or high value samples was exemplified using plasma from patients with SARS-CoV-2 infection and normal controls. 1H NMR spect... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Viral dynamics of acute SARS-CoV-2 infection and applications to diagnostic and public health strategies
### Response:
| SARS-CoV-2 infections are characterized by viral proliferation and clearance phases and can be followed by low-level persistent viral RNA shedding. The dynamics of viral RNA concentration, particularly in the early stages of infection, can inform clinical measures and interventions such as test-based screening. We used... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Rapid Detection of SARS-CoV-2 using Graphene-based IoT Integrated Advanced Electrochemical Biosensor
### Response:
| Unique characteristics like large surface area, excellent conductivity, functionality, ease of fabrication, etc., of graphene and its derivatives, have been extensively studied as potential candidates in healthcare applications. They have been utilized as a potential nanomaterial in biosensor fabrication for commercial... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Reciprocating-flowing on-a-chip enables ultra-fast immunobinding for multiplexed rapid ELISA detection of SARS-CoV-2 antibody
### Response:
| The worldwide epidemic of novel coronavirus disease (COVID-19) has led to a strong demand for highly efficient immunobinding to achieve rapid and accurate on-site detection of SARS-CoV-2 antibodies. However, hour-scale time-consumption is usually required to ensure the adequacy of immunobinding on expensive large instr... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Diagnostic performance of CerTest and Panbio antigen rapid diagnostic tests to diagnose SARS-CoV-2 infection
### Response:
| OBJECTIVES: Antigen rapid diagnostic tests (Ag-RDT) have been developed as reliable tools to control the SARS-CoV-2 pandemic. The objective of our study was to evaluate the diagnostic performance of two Ag-RDTs. METHODS: We evaluated CerTest SARS-CoV-2 Ag One Step Card Test and Panbio COVID-19 Ag Rapid Test Device Ag-R... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Stability of SARS-CoV-2 RNA in Viral Lysis Buffer Stored at Different Temperatures
### Response:
| Objectives The present COVID-19 pandemic resulted in an increased need for molecular diagnostic testing Delay in the specimen processing and suboptimal storage of suspected samples in laboratories leads to degradation of SARS-CoV-2 viral RNA Viral lysis buffers from RNA extraction kits have the potential to stabilize RN... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Detection of SARS-CoV-2 in respiratory samples from cats in the UK associated with human-to-cat transmission
### Response:
| OBJECTIVES: The aim of the study was to find evidence of SARS-CoV-2 infection in UK cats. DESIGN: Tissue samples were tested for SARS-CoV-2 antigen using immunofluorescence and for viral RNA by in situ hybridisation. A set of 387 oropharyngeal swabs that had been submitted for routine respiratory pathogen testing was t... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Error Correction Codes for COVID-19 Virus and Antibody Testing: Using Pooled Testing to Increase Test Reliability
### Response:
| We consider a novel method to increase the reliability of COVID-19 virus or antibody tests by using specially designed pooled testings. Instead of testing nasal swab or blood samples from individual persons, we propose to test mixtures of samples from many individuals. The pooled sample testing method proposed in this ... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Epidemiological evaluation of sewage surveillance as a tool to detect the presence of COVID-19 cases in a low case load setting
### Response:
| In low prevalence settings the development of sensitive and specific quantitative Reverse Transcription Polymerase Chain Reaction tests to detect SARS-CoV − 2 (the virus causing COVID-19) in sewage presents the possibility of using sewage sampling as a diagnostic test for the presence of infected people in the catchmen... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Feasibility and accuracy of a novel saliva sampling method for large-scale SARS-CoV-2 screening in children < 12 years of age
### Response:
| Objectives Children have been disproportionately affected during the COVID-19 pandemic. Novel test strategies are urgently needed to ensure safe operation of schools and childcare institutions and to avoid prolonged closures. Methods A weekly SARS-CoV-2 sentinel study in primary schools, kindergartens and childcare fac... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Ultrasensitive and visual detection of SARS-CoV-2 using all-in-one dual CRISPR-Cas12a assay
### Response:
| The recent outbreak of novel coronavirus (SARS-CoV-2) causing COVID-19 disease spreads rapidly in the world. Rapid and early detection of SARS-CoV-2 facilitates early intervention and prevents the disease spread. Here, we present an All-In-One Dual CRISPR-Cas12a (AIOD-CRISPR) assay for one-pot, ultrasensitive, and visu... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Sample-to-Answer and Routine Real-Time RT-PCR: A Comparison of Different Platforms for SARS-CoV-2 Detection
### Response:
| Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is spreading all over the world and has caused millions of deaths. Several sample-to-answer platforms, including Cepheid Xpert Xpress SARS-CoV-2 (Xpert Xpress), have received emergency use authorization for SARS-CoV-2 nucleic acid detection as a point of care... |
### Instruction:
Please summerize the given abstract to a title
### Response:
shinyCurves, a shiny web application to analyse multisource qPCR amplification data: a COVID 19 case study
### Response:
| Quantitative, reverse transcription polymerase chain reaction (qRT-PCR) has been the gold-standard tool for viral detection during the SARS-CoV-2 pandemic. However, the desperate rush for a quick diagnosis led the use of very different types of machines and proprietary software, leading to an unbearable complexity of d... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Feasibility and utility of facemask sampling in the detection of SARS-CoV-2 during an ongoing pandemic
### Response:
| Easy access to screening for timely identification and isolation of infectious COVID-19 patients remains crucial in sustaining the international efforts to control COVID-19 spread. A major barrier limiting broad-based screening is the lack of a simple, rapid, and cost-effective COVID-19 testing method. We evaluated the... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Evaluation of mobile real-time polymerase chain reaction tests for the detection of severe acute respiratory syndrome coronavirus 2
### Response:
| The coronavirus disease 2019 (COVID-19) pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), calls for prompt and accurate diagnosis and rapid turnaround time for test results to limit transmission. Here, we evaluated two independent molecular assays, the Biomeme SARS-CoV-2 test, and the Pr... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Verification and Validation of SARS-CoV-2 Assay Performance on the Abbott m2000 and Alinity m Systems
### Response:
| We verified the analytical performance of the Abbott RealTime SARS-CoV-2 assay on the m2000 system and compared its clinical performance to the CDC 2019-nCoV Real-Time PCR Diagnostic Panel and the ThermoFisher TaqPath RT-PCR COVID-19 kit. We also performed a bridging study comparing the RealTime SARS-CoV-2 assay with t... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Detection of SARS-CoV-2 in air and on surfaces in rooms of infected nursing home residents
### Response:
| There is an ongoing debate on airborne transmission of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) as a risk factor for infection. In this study, the level of SARS-CoV-2 in air and on surfaces of SARS-CoV-2 infected nursing home residents was assessed to gain insight in potential transmission routes. D... |
### Instruction:
Please summerize the given abstract to a title
### Response:
A Novel Approach to the Bioluminescent Detection of the SARS-CoV-2 ORF1ab Gene by Coupling Isothermal RNA Reverse Transcription Amplification with a Digital PCR Approach
### Response:
| The COVID-19 pandemic caused by the SARS-CoV-2 virus, which first emerged in December 2019, represents an ongoing global public health emergency. Here, we developed an improved and highly sensitive approach to SARS-CoV-2 detection via coupling bioluminescence in real-time (BART) and reverse-transcriptase loop-mediated ... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Ad hoc laboratory-based surveillance of SARS-CoV-2 by real-time RT-PCR using minipools of RNA prepared from routine respiratory samples
### Response:
| We report a laboratory-based surveillance for SARS-CoV-2 using minipools of respiratory samples submitted for routine diagnostics. We tested a total of 70 minipools resembling 700 samples shortly before the upsurge of cases in Germany. We identified one SARS-CoV-2 positive patient. Our approach proved its concept, is e... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Prolonged Detection of SARS CoV2 RNA in Extracellular Vesicles in Nasal Swab RT-PCR Negative Patients
### Response:
| Background: There is a prolonged RT PCR positivity seen in COVID-19 infected patients up to 2 to 3 months. It is assumed that this virus is usually non-infective but there are hardly any study on the reactivation of this virus within the respiratory tract. We aim to investigate the presence of viral particles inside Ex... |
### Instruction:
Please summerize the given abstract to a title
### Response:
SPECIFIC DETECTION OF SARS-COV-2 VARIANTS B.1.1.7 (ALPHA) AND B.1.617.2 (DELTA) USING A ONE-STEP QUANTITATIVE PCR ASSAY
### Response:
| In this report, we describe the development of an RT-qPCR assay, termed Alpha Delta assay, which can detect SARS-COV-2 (SC-2) and distinguish between the Alpha (B.1.1.7) and Delta (B.1.617.2) variants. The Alpha- and Delta-specific reactions in the assay target mutations that are strongly linked to the target variant. ... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Wastewater based epidemiology as a silent sentinel of the trend of SARS-CoV-2 circulation in the community in central Argentina
### Response:
| Monitoring wastewater for the traces of viruses allows effective surveillance of entire communities, including symptomatic and asymptomatic infected individuals, providing information on whether a specific pathogen is circulating in a population. In the context of the COVID-19 pandemic, 261 wastewater samples from six ... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Monitoring waves of the COVID-19 pandemic: Inferences from WWTPs of different sizes
### Response:
| Wastewater based epidemiology was employed to track the spread of SARS-CoV-2 within the sewershed areas of 10 wastewater treatment plants (WWTPs) in Catalonia, Spain. A total of 185 WWTPs inflow samples were collected over the period consisting of both the first wave (mid-March to June) and the second wave (July to Nov... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Comparison of SARS-CoV2 N gene real-time RT-PCR targets and commercially available mastermixes
### Response:
| We aim to test four one-step RT real-time mastermix options for use in SARS-CoV2 real-time PCR, with three primer/probe assays targeting the N gene. The lower limit of detection is determined using a SARS CoV2 N gene RNA transcript dilution series (to 1 copy/µl) and verified using 74 nose and throat swabs. The N2 assay... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Comparison of a Point-of-Care Assay and a High-Complexity Assay for Detection of SARS-CoV-2 RNA
### Response:
| BACKGROUND: Numerous nucleic acid amplification assays utilizing different target genes of the SARS-CoV-2 genome have received emergency use authorization (EUA) by the United States Food and Drug Administration (FDA). Limited data are available comparing the test performance characteristics of these assays. METHODS: A ... |
### Instruction:
Please summerize the given abstract to a title
### Response:
High Efficacy of Saliva in Detecting SARS-CoV-2 by RT-PCR in Adults and Children
### Response:
| Rising demands for repetitive SARS-CoV-2 screens and mass testing necessitate additional test strategies. Saliva may serve as an alternative to nasopharyngeal swab (NPS) as its collection is simple, non-invasive and amenable for mass- and home testing, but its rigorous validation, particularly in children, is missing. ... |
### Instruction:
Please summerize the given abstract to a title
### Response:
Detection and infectivity potential of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) environmental contamination in isolation units and quarantine facilities
### Response:
| OBJECTIVES: Environmental surfaces have been suggested as likely contributors to the transmission of COVID-19. This study assessed the infectivity of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) contamination on surfaces and objects in hospital isolation units and a quarantine hotel. METHODS: SARS-CoV-2... |
End of preview. Expand in Data Studio
- Downloads last month
- 2