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Update templates/index.html
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templates/index.html
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| 1 |
+
<!DOCTYPE html>
|
| 2 |
+
<html lang="zh-CN">
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| 3 |
+
<head>
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| 4 |
+
<meta charset="UTF-8">
|
| 5 |
+
<meta name="viewport" content="width=device-width, initial-scale=1.0">
|
| 6 |
+
<title>qPCR引物设计工具</title>
|
| 7 |
+
<style>
|
| 8 |
+
* {
|
| 9 |
+
margin: 0;
|
| 10 |
+
padding: 0;
|
| 11 |
+
box-sizing: border-box;
|
| 12 |
+
}
|
| 13 |
+
|
| 14 |
+
body {
|
| 15 |
+
font-family: 'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;
|
| 16 |
+
background: linear-gradient(135deg, #667eea 0%, #764ba2 100%);
|
| 17 |
+
min-height: 100vh;
|
| 18 |
+
padding: 20px;
|
| 19 |
+
}
|
| 20 |
+
|
| 21 |
+
.container {
|
| 22 |
+
max-width: 1200px;
|
| 23 |
+
margin: 0 auto;
|
| 24 |
+
background: white;
|
| 25 |
+
border-radius: 15px;
|
| 26 |
+
box-shadow: 0 20px 40px rgba(0,0,0,0.1);
|
| 27 |
+
overflow: hidden;
|
| 28 |
+
}
|
| 29 |
+
|
| 30 |
+
.header {
|
| 31 |
+
background: linear-gradient(135deg, #4facfe 0%, #00f2fe 100%);
|
| 32 |
+
color: white;
|
| 33 |
+
padding: 30px;
|
| 34 |
+
text-align: center;
|
| 35 |
+
}
|
| 36 |
+
|
| 37 |
+
.header h1 {
|
| 38 |
+
font-size: 2.5em;
|
| 39 |
+
margin-bottom: 10px;
|
| 40 |
+
}
|
| 41 |
+
|
| 42 |
+
.header p {
|
| 43 |
+
font-size: 1.1em;
|
| 44 |
+
opacity: 0.9;
|
| 45 |
+
}
|
| 46 |
+
|
| 47 |
+
.form-section {
|
| 48 |
+
padding: 40px;
|
| 49 |
+
}
|
| 50 |
+
|
| 51 |
+
.tab-container {
|
| 52 |
+
margin-bottom: 30px;
|
| 53 |
+
}
|
| 54 |
+
|
| 55 |
+
.tab-buttons {
|
| 56 |
+
display: flex;
|
| 57 |
+
border-bottom: 2px solid #e1e5e9;
|
| 58 |
+
margin-bottom: 20px;
|
| 59 |
+
}
|
| 60 |
+
|
| 61 |
+
.tab-button {
|
| 62 |
+
padding: 12px 24px;
|
| 63 |
+
background: none;
|
| 64 |
+
border: none;
|
| 65 |
+
cursor: pointer;
|
| 66 |
+
font-size: 16px;
|
| 67 |
+
font-weight: 600;
|
| 68 |
+
color: #666;
|
| 69 |
+
border-bottom: 3px solid transparent;
|
| 70 |
+
transition: all 0.3s;
|
| 71 |
+
}
|
| 72 |
+
|
| 73 |
+
.tab-button.active {
|
| 74 |
+
color: #4facfe;
|
| 75 |
+
border-bottom-color: #4facfe;
|
| 76 |
+
}
|
| 77 |
+
|
| 78 |
+
.tab-content {
|
| 79 |
+
display: none;
|
| 80 |
+
}
|
| 81 |
+
|
| 82 |
+
.tab-content.active {
|
| 83 |
+
display: block;
|
| 84 |
+
}
|
| 85 |
+
|
| 86 |
+
.batch-input {
|
| 87 |
+
margin-bottom: 20px;
|
| 88 |
+
}
|
| 89 |
+
|
| 90 |
+
.batch-textarea {
|
| 91 |
+
width: 100%;
|
| 92 |
+
min-height: 150px;
|
| 93 |
+
padding: 15px;
|
| 94 |
+
border: 2px solid #e1e5e9;
|
| 95 |
+
border-radius: 8px;
|
| 96 |
+
font-size: 14px;
|
| 97 |
+
font-family: 'Courier New', monospace;
|
| 98 |
+
resize: vertical;
|
| 99 |
+
}
|
| 100 |
+
|
| 101 |
+
.batch-help {
|
| 102 |
+
background: #f8f9fa;
|
| 103 |
+
padding: 15px;
|
| 104 |
+
border-radius: 8px;
|
| 105 |
+
margin-bottom: 20px;
|
| 106 |
+
font-size: 14px;
|
| 107 |
+
color: #666;
|
| 108 |
+
}
|
| 109 |
+
|
| 110 |
+
.export-section {
|
| 111 |
+
background: #f8f9fa;
|
| 112 |
+
padding: 20px;
|
| 113 |
+
border-radius: 8px;
|
| 114 |
+
margin-top: 20px;
|
| 115 |
+
}
|
| 116 |
+
|
| 117 |
+
.export-buttons {
|
| 118 |
+
display: flex;
|
| 119 |
+
gap: 10px;
|
| 120 |
+
flex-wrap: wrap;
|
| 121 |
+
}
|
| 122 |
+
|
| 123 |
+
.export-btn {
|
| 124 |
+
background: #28a745;
|
| 125 |
+
color: white;
|
| 126 |
+
padding: 10px 20px;
|
| 127 |
+
border: none;
|
| 128 |
+
border-radius: 6px;
|
| 129 |
+
cursor: pointer;
|
| 130 |
+
font-size: 14px;
|
| 131 |
+
font-weight: 600;
|
| 132 |
+
transition: background 0.3s;
|
| 133 |
+
}
|
| 134 |
+
|
| 135 |
+
.export-btn:hover {
|
| 136 |
+
background: #218838;
|
| 137 |
+
}
|
| 138 |
+
|
| 139 |
+
.export-btn:disabled {
|
| 140 |
+
background: #6c757d;
|
| 141 |
+
cursor: not-allowed;
|
| 142 |
+
}
|
| 143 |
+
|
| 144 |
+
.batch-progress {
|
| 145 |
+
display: none;
|
| 146 |
+
margin: 20px 0;
|
| 147 |
+
}
|
| 148 |
+
|
| 149 |
+
.progress-bar {
|
| 150 |
+
width: 100%;
|
| 151 |
+
height: 20px;
|
| 152 |
+
background: #e1e5e9;
|
| 153 |
+
border-radius: 10px;
|
| 154 |
+
overflow: hidden;
|
| 155 |
+
}
|
| 156 |
+
|
| 157 |
+
.progress-fill {
|
| 158 |
+
height: 100%;
|
| 159 |
+
background: linear-gradient(90deg, #4facfe, #00f2fe);
|
| 160 |
+
width: 0%;
|
| 161 |
+
transition: width 0.3s;
|
| 162 |
+
}
|
| 163 |
+
|
| 164 |
+
.progress-text {
|
| 165 |
+
text-align: center;
|
| 166 |
+
margin-top: 10px;
|
| 167 |
+
font-weight: 600;
|
| 168 |
+
}
|
| 169 |
+
|
| 170 |
+
.batch-results {
|
| 171 |
+
display: none;
|
| 172 |
+
}
|
| 173 |
+
|
| 174 |
+
.result-summary {
|
| 175 |
+
background: white;
|
| 176 |
+
padding: 20px;
|
| 177 |
+
border-radius: 8px;
|
| 178 |
+
margin-bottom: 20px;
|
| 179 |
+
display: flex;
|
| 180 |
+
justify-content: space-between;
|
| 181 |
+
align-items: center;
|
| 182 |
+
}
|
| 183 |
+
|
| 184 |
+
.summary-stats {
|
| 185 |
+
display: flex;
|
| 186 |
+
gap: 30px;
|
| 187 |
+
}
|
| 188 |
+
|
| 189 |
+
.stat-item {
|
| 190 |
+
text-align: center;
|
| 191 |
+
}
|
| 192 |
+
|
| 193 |
+
.stat-number {
|
| 194 |
+
font-size: 24px;
|
| 195 |
+
font-weight: bold;
|
| 196 |
+
color: #4facfe;
|
| 197 |
+
}
|
| 198 |
+
|
| 199 |
+
.stat-label {
|
| 200 |
+
font-size: 12px;
|
| 201 |
+
color: #666;
|
| 202 |
+
text-transform: uppercase;
|
| 203 |
+
}
|
| 204 |
+
|
| 205 |
+
.failed-genes {
|
| 206 |
+
background: #fff3cd;
|
| 207 |
+
border: 1px solid #ffeaa7;
|
| 208 |
+
padding: 15px;
|
| 209 |
+
border-radius: 8px;
|
| 210 |
+
margin-bottom: 20px;
|
| 211 |
+
}
|
| 212 |
+
|
| 213 |
+
.failed-gene {
|
| 214 |
+
margin-bottom: 10px;
|
| 215 |
+
padding: 8px;
|
| 216 |
+
background: white;
|
| 217 |
+
border-radius: 4px;
|
| 218 |
+
border-left: 3px solid #e17055;
|
| 219 |
+
}
|
| 220 |
+
|
| 221 |
+
.form-group {
|
| 222 |
+
margin-bottom: 25px;
|
| 223 |
+
}
|
| 224 |
+
|
| 225 |
+
label {
|
| 226 |
+
display: block;
|
| 227 |
+
margin-bottom: 8px;
|
| 228 |
+
font-weight: 600;
|
| 229 |
+
color: #333;
|
| 230 |
+
}
|
| 231 |
+
|
| 232 |
+
input, select {
|
| 233 |
+
width: 100%;
|
| 234 |
+
padding: 12px 15px;
|
| 235 |
+
border: 2px solid #e1e5e9;
|
| 236 |
+
border-radius: 8px;
|
| 237 |
+
font-size: 16px;
|
| 238 |
+
transition: border-color 0.3s;
|
| 239 |
+
}
|
| 240 |
+
|
| 241 |
+
input:focus, select:focus {
|
| 242 |
+
outline: none;
|
| 243 |
+
border-color: #4facfe;
|
| 244 |
+
}
|
| 245 |
+
|
| 246 |
+
.btn {
|
| 247 |
+
background: linear-gradient(135deg, #667eea 0%, #764ba2 100%);
|
| 248 |
+
color: white;
|
| 249 |
+
padding: 15px 30px;
|
| 250 |
+
border: none;
|
| 251 |
+
border-radius: 8px;
|
| 252 |
+
font-size: 16px;
|
| 253 |
+
font-weight: 600;
|
| 254 |
+
cursor: pointer;
|
| 255 |
+
transition: transform 0.2s;
|
| 256 |
+
width: 100%;
|
| 257 |
+
}
|
| 258 |
+
|
| 259 |
+
.btn:hover {
|
| 260 |
+
transform: translateY(-2px);
|
| 261 |
+
}
|
| 262 |
+
|
| 263 |
+
.btn:disabled {
|
| 264 |
+
opacity: 0.6;
|
| 265 |
+
cursor: not-allowed;
|
| 266 |
+
transform: none;
|
| 267 |
+
}
|
| 268 |
+
|
| 269 |
+
.loading {
|
| 270 |
+
display: none;
|
| 271 |
+
text-align: center;
|
| 272 |
+
padding: 20px;
|
| 273 |
+
}
|
| 274 |
+
|
| 275 |
+
.spinner {
|
| 276 |
+
border: 4px solid #f3f3f3;
|
| 277 |
+
border-top: 4px solid #4facfe;
|
| 278 |
+
border-radius: 50%;
|
| 279 |
+
width: 40px;
|
| 280 |
+
height: 40px;
|
| 281 |
+
animation: spin 1s linear infinite;
|
| 282 |
+
margin: 0 auto 10px;
|
| 283 |
+
}
|
| 284 |
+
|
| 285 |
+
@keyframes spin {
|
| 286 |
+
0% { transform: rotate(0deg); }
|
| 287 |
+
100% { transform: rotate(360deg); }
|
| 288 |
+
}
|
| 289 |
+
|
| 290 |
+
.results {
|
| 291 |
+
display: none;
|
| 292 |
+
padding: 40px;
|
| 293 |
+
background: #f8f9fa;
|
| 294 |
+
}
|
| 295 |
+
|
| 296 |
+
.gene-info {
|
| 297 |
+
background: white;
|
| 298 |
+
padding: 20px;
|
| 299 |
+
border-radius: 8px;
|
| 300 |
+
margin-bottom: 30px;
|
| 301 |
+
border-left: 4px solid #4facfe;
|
| 302 |
+
}
|
| 303 |
+
|
| 304 |
+
.primer-card {
|
| 305 |
+
background: white;
|
| 306 |
+
border-radius: 8px;
|
| 307 |
+
padding: 20px;
|
| 308 |
+
margin-bottom: 20px;
|
| 309 |
+
box-shadow: 0 2px 10px rgba(0,0,0,0.1);
|
| 310 |
+
}
|
| 311 |
+
|
| 312 |
+
.primer-header {
|
| 313 |
+
display: flex;
|
| 314 |
+
justify-content: space-between;
|
| 315 |
+
align-items: center;
|
| 316 |
+
margin-bottom: 15px;
|
| 317 |
+
}
|
| 318 |
+
|
| 319 |
+
.primer-id {
|
| 320 |
+
background: #4facfe;
|
| 321 |
+
color: white;
|
| 322 |
+
padding: 5px 15px;
|
| 323 |
+
border-radius: 20px;
|
| 324 |
+
font-weight: 600;
|
| 325 |
+
}
|
| 326 |
+
|
| 327 |
+
.product-size {
|
| 328 |
+
background: #e8f5e8;
|
| 329 |
+
color: #2d5a2d;
|
| 330 |
+
padding: 5px 15px;
|
| 331 |
+
border-radius: 20px;
|
| 332 |
+
font-weight: 600;
|
| 333 |
+
}
|
| 334 |
+
|
| 335 |
+
.primer-sequences {
|
| 336 |
+
display: grid;
|
| 337 |
+
grid-template-columns: 1fr 1fr;
|
| 338 |
+
gap: 20px;
|
| 339 |
+
margin-bottom: 15px;
|
| 340 |
+
}
|
| 341 |
+
|
| 342 |
+
.sequence-box {
|
| 343 |
+
background: #f8f9fa;
|
| 344 |
+
padding: 15px;
|
| 345 |
+
border-radius: 6px;
|
| 346 |
+
border-left: 3px solid #4facfe;
|
| 347 |
+
}
|
| 348 |
+
|
| 349 |
+
.sequence-label {
|
| 350 |
+
font-weight: 600;
|
| 351 |
+
color: #666;
|
| 352 |
+
margin-bottom: 5px;
|
| 353 |
+
}
|
| 354 |
+
|
| 355 |
+
.sequence {
|
| 356 |
+
font-family: 'Courier New', monospace;
|
| 357 |
+
font-size: 14px;
|
| 358 |
+
word-break: break-all;
|
| 359 |
+
background: white;
|
| 360 |
+
padding: 8px;
|
| 361 |
+
border-radius: 4px;
|
| 362 |
+
border: 1px solid #e1e5e9;
|
| 363 |
+
}
|
| 364 |
+
|
| 365 |
+
.tm-info {
|
| 366 |
+
display: flex;
|
| 367 |
+
justify-content: space-between;
|
| 368 |
+
font-size: 14px;
|
| 369 |
+
color: #666;
|
| 370 |
+
}
|
| 371 |
+
|
| 372 |
+
.error {
|
| 373 |
+
background: #fee;
|
| 374 |
+
color: #c33;
|
| 375 |
+
padding: 15px;
|
| 376 |
+
border-radius: 8px;
|
| 377 |
+
border-left: 4px solid #c33;
|
| 378 |
+
margin: 20px 0;
|
| 379 |
+
}
|
| 380 |
+
|
| 381 |
+
@media (max-width: 768px) {
|
| 382 |
+
.primer-sequences {
|
| 383 |
+
grid-template-columns: 1fr;
|
| 384 |
+
}
|
| 385 |
+
|
| 386 |
+
.primer-header {
|
| 387 |
+
flex-direction: column;
|
| 388 |
+
gap: 10px;
|
| 389 |
+
}
|
| 390 |
+
}
|
| 391 |
+
</style>
|
| 392 |
+
</head>
|
| 393 |
+
<body>
|
| 394 |
+
<div class="container">
|
| 395 |
+
<div class="header">
|
| 396 |
+
<h1>🧬 qPCR引物设计工具</h1>
|
| 397 |
+
<p>输入基因名称和物种，自动设计跨越外显子交界点的qPCR引物</p>
|
| 398 |
+
<p style="margin-top: 15px; font-size: 0.9em; opacity: 0.8;">Developed by PharC | Contact: pc794797023@163.com</p>
|
| 399 |
+
</div>
|
| 400 |
+
|
| 401 |
+
<div class="form-section">
|
| 402 |
+
<div class="tab-container">
|
| 403 |
+
<div class="tab-buttons">
|
| 404 |
+
<button type="button" class="tab-button active" onclick="switchTab('single')">
|
| 405 |
+
🧬 单个基因
|
| 406 |
+
</button>
|
| 407 |
+
<button type="button" class="tab-button" onclick="switchTab('batch')">
|
| 408 |
+
📋 批量处理
|
| 409 |
+
</button>
|
| 410 |
+
</div>
|
| 411 |
+
|
| 412 |
+
<!-- 单个基因设计 -->
|
| 413 |
+
<div id="single-tab" class="tab-content active">
|
| 414 |
+
<form id="primerForm">
|
| 415 |
+
<div class="form-group">
|
| 416 |
+
<label for="geneSymbol">基因名称 *</label>
|
| 417 |
+
<input type="text" id="geneSymbol" name="geneSymbol" placeholder="例如: GAPDH, Gpr34" required>
|
| 418 |
+
</div>
|
| 419 |
+
|
| 420 |
+
<div class="form-group">
|
| 421 |
+
<label for="species">物种</label>
|
| 422 |
+
<select id="species" name="species">
|
| 423 |
+
<option value="human">人类 (Human)</option>
|
| 424 |
+
<option value="Mus musculus">小鼠 (Mouse)</option>
|
| 425 |
+
<option value="Rattus norvegicus">大鼠 (Rat)</option>
|
| 426 |
+
<option value="Drosophila melanogaster">果蝇 (Drosophila)</option>
|
| 427 |
+
<option value="Caenorhabditis elegans">线虫 (C. elegans)</option>
|
| 428 |
+
<option value="Danio rerio">斑马鱼 (Zebrafish)</option>
|
| 429 |
+
</select>
|
| 430 |
+
</div>
|
| 431 |
+
|
| 432 |
+
<button type="submit" class="btn" id="submitBtn">
|
| 433 |
+
🔬 设计引物
|
| 434 |
+
</button>
|
| 435 |
+
</form>
|
| 436 |
+
</div>
|
| 437 |
+
|
| 438 |
+
<!-- 批量基因设计 -->
|
| 439 |
+
<div id="batch-tab" class="tab-content">
|
| 440 |
+
<form id="batchForm">
|
| 441 |
+
<div class="batch-help">
|
| 442 |
+
💡 <strong>使用说明：</strong>每行输入一个基因名称，支持多种格式：
|
| 443 |
+
<br>• 一行一个基因：GAPDH
|
| 444 |
+
<br>• 逗号分隔：GAPDH, ACTB, TUBB3
|
| 445 |
+
<br>• 空格分隔：GAPDH ACTB TUBB3
|
| 446 |
+
</div>
|
| 447 |
+
|
| 448 |
+
<div class="form-group">
|
| 449 |
+
<label for="batchGenes">基因列表 *</label>
|
| 450 |
+
<textarea
|
| 451 |
+
id="batchGenes"
|
| 452 |
+
class="batch-textarea"
|
| 453 |
+
placeholder="请输入基因名称，每行一个或用逗号/空格分隔： GAPDH ACTB TUBB3 或者：GAPDH, ACTB, TUBB3"
|
| 454 |
+
required
|
| 455 |
+
></textarea>
|
| 456 |
+
</div>
|
| 457 |
+
|
| 458 |
+
<div class="form-group">
|
| 459 |
+
<label for="batchSpecies">物种</label>
|
| 460 |
+
<select id="batchSpecies" name="batchSpecies">
|
| 461 |
+
<option value="human">人类 (Human)</option>
|
| 462 |
+
<option value="Mus musculus">小鼠 (Mouse)</option>
|
| 463 |
+
<option value="Rattus norvegicus">大鼠 (Rat)</option>
|
| 464 |
+
<option value="Drosophila melanogaster">果蝇 (Drosophila)</option>
|
| 465 |
+
<option value="Caenorhabditis elegans">线虫 (C. elegans)</option>
|
| 466 |
+
<option value="Danio rerio">斑马鱼 (Zebrafish)</option>
|
| 467 |
+
</select>
|
| 468 |
+
</div>
|
| 469 |
+
|
| 470 |
+
<button type="submit" class="btn" id="batchSubmitBtn">
|
| 471 |
+
🚀 批量设计引物
|
| 472 |
+
</button>
|
| 473 |
+
</form>
|
| 474 |
+
|
| 475 |
+
<div class="batch-progress" id="batchProgress">
|
| 476 |
+
<div class="progress-bar">
|
| 477 |
+
<div class="progress-fill" id="progressFill"></div>
|
| 478 |
+
</div>
|
| 479 |
+
<div class="progress-text" id="progressText">正在处理: 0/0</div>
|
| 480 |
+
</div>
|
| 481 |
+
</div>
|
| 482 |
+
</div>
|
| 483 |
+
</div>
|
| 484 |
+
|
| 485 |
+
<div class="loading" id="loading">
|
| 486 |
+
<div class="spinner"></div>
|
| 487 |
+
<p>正在设计引物，请稍候...</p>
|
| 488 |
+
</div>
|
| 489 |
+
|
| 490 |
+
<div class="results" id="results">
|
| 491 |
+
<div class="gene-info" id="geneInfo"></div>
|
| 492 |
+
<div id="primerResults"></div>
|
| 493 |
+
|
| 494 |
+
<div class="export-section" id="exportSection" style="display: none;">
|
| 495 |
+
<h4>📥 导出结果</h4>
|
| 496 |
+
<p>选择导出格式：</p>
|
| 497 |
+
<div class="export-buttons">
|
| 498 |
+
<button class="export-btn" onclick="exportResults('excel')">
|
| 499 |
+
📊 Excel格式
|
| 500 |
+
</button>
|
| 501 |
+
<button class="export-btn" onclick="exportResults('csv')">
|
| 502 |
+
📄 CSV格式
|
| 503 |
+
</button>
|
| 504 |
+
<button class="export-btn" onclick="exportResults('json')">
|
| 505 |
+
🔧 JSON格式
|
| 506 |
+
</button>
|
| 507 |
+
</div>
|
| 508 |
+
</div>
|
| 509 |
+
</div>
|
| 510 |
+
|
| 511 |
+
<div class="batch-results" id="batchResults">
|
| 512 |
+
<div class="result-summary" id="resultSummary"></div>
|
| 513 |
+
<div id="failedGenes"></div>
|
| 514 |
+
<div id="batchPrimerResults"></div>
|
| 515 |
+
|
| 516 |
+
<div class="export-section">
|
| 517 |
+
<h4>📥 导出批量结果</h4>
|
| 518 |
+
<p>选择导出格式：</p>
|
| 519 |
+
<div class="export-buttons">
|
| 520 |
+
<button class="export-btn" onclick="exportBatchResults('excel')">
|
| 521 |
+
📊 Excel格式
|
| 522 |
+
</button>
|
| 523 |
+
<button class="export-btn" onclick="exportBatchResults('csv')">
|
| 524 |
+
📄 CSV格式
|
| 525 |
+
</button>
|
| 526 |
+
<button class="export-btn" onclick="exportBatchResults('json')">
|
| 527 |
+
🔧 JSON格式
|
| 528 |
+
</button>
|
| 529 |
+
</div>
|
| 530 |
+
</div>
|
| 531 |
+
</div>
|
| 532 |
+
</div>
|
| 533 |
+
|
| 534 |
+
<script src="{{ url_for('static', filename='app.js') }}"></script>
|
| 535 |
+
</body>
|
| 536 |
+
</html>
|