diff --git "a/data/ppi_llm/ppi_l1_dataset.jsonl" "b/data/ppi_llm/ppi_l1_dataset.jsonl" new file mode 100644--- /dev/null +++ "b/data/ppi_llm/ppi_l1_dataset.jsonl" @@ -0,0 +1,1200 @@ +{"question_id": "PPIL1-0000", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: HNRPLL (A0A0S2Z5U3)\n\nProtein 2: SRSF11 (Q05519)\n Function: May function in pre-mRNA splicing\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between HNRPLL and SRSF11.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 307, "gene_symbol_1": "HNRPLL", "gene_symbol_2": "SRSF11", "detection_method": null}} +{"question_id": "PPIL1-0001", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: KRTAP5-9 (P26371)\n Function: In the hair cortex, hair keratin intermediate filaments are embedded in an interfilamentous matrix, consisting of hair keratin-associated protein (KRTAP), which are essential for the formation of a ri\n\nProtein 2: SLC25A10 (Q9UBX3)\n Function: Catalyzes the electroneutral exchange or flux of physiologically important metabolites such as dicarboxylates (malonate, malate, succinate), inorganic sulfur-containing anions, and phosphate, across t\n Location: Mitochondrion inner membrane\n\nEvidence: Available evidence does not support a physical interaction between KRTAP5-9 and SLC25A10.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 586, "gene_symbol_1": "KRTAP5-9", "gene_symbol_2": "SLC25A10", "detection_method": null}} +{"question_id": "PPIL1-0002", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: FHL5 (Q5TD97)\n Function: May be involved in the regulation of spermatogenesis. Stimulates CREM transcriptional activity in a phosphorylation-independent manner\n Location: Nucleus\n\nProtein 2: BANP (B4DE54)\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between FHL5 and BANP.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 174, "gene_symbol_1": "FHL5", "gene_symbol_2": "BANP", "detection_method": null}} +{"question_id": "PPIL1-0003", "task": "ppi-l1", "split": "fewshot", "difficulty": "hard", "context_text": "Protein 1: CASP8 (Q14790)\n Function: Thiol protease that plays a key role in programmed cell death by acting as a molecular switch for apoptosis, necroptosis and pyroptosis, and is required to prevent tissue damage during embryonic devel\n Location: Cytoplasm\n\nProtein 2: FADD (Q13158)\n Function: Apoptotic adapter molecule that recruits caspases CASP8 or CASP10 to the activated FAS/CD95 or TNFRSF1A/TNFR-1 receptors (PubMed:16762833, PubMed:19118384, PubMed:20935634, PubMed:23955153, PubMed:240\n Location: Cytoplasm\n\nEvidence: Available evidence does not support a physical interaction between CASP8 and FADD.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "gold", "result_id": 81, "gene_symbol_1": "CASP8", "gene_symbol_2": "FADD", "detection_method": null}} +{"question_id": "PPIL1-0004", "task": "ppi-l1", "split": "fewshot", "difficulty": "hard", "context_text": "Protein 1: SMUG1 (Q53HV7)\n Function: Recognizes base lesions in the genome and initiates base excision DNA repair. Acts as a monofunctional DNA glycosylase specific for uracil (U) residues in DNA with a preference for single-stranded DNA\n Location: Nucleus\n\nProtein 2: CDC23 (Q9UJX2)\n Function: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle (PubMed:18485873)\n\nEvidence: Available evidence does not support a physical interaction between SMUG1 and CDC23.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 568, "gene_symbol_1": "SMUG1", "gene_symbol_2": "CDC23", "detection_method": null}} +{"question_id": "PPIL1-0005", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: BTRC (A0A0S2Z4Q5)\n\nProtein 2: TACC1 (O75410)\n Function: Involved in transcription regulation induced by nuclear receptors, including in T3 thyroid hormone and all-trans retinoic acid pathways (PubMed:20078863). Might promote the nuclear localization of the\n Location: Cytoplasm\n\nEvidence: Available evidence does not support a physical interaction between BTRC and TACC1.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 193, "gene_symbol_1": "BTRC", "gene_symbol_2": "TACC1", "detection_method": null}} +{"question_id": "PPIL1-0006", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: FATE1 (Q969F0)\n Function: Involved in the regulation of endoplasmic reticulum (ER)-mitochondria coupling. Negatively regulates the ER-mitochondria distance and Ca(2+) transfer from ER to mitochondria possibly implicating it in\n Location: Mitochondrion\n\nProtein 2: SEC22C (A0A0S2Z4S8)\n\nEvidence: Available evidence does not support a physical interaction between FATE1 and SEC22C.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 590, "gene_symbol_1": "FATE1", "gene_symbol_2": "SEC22C", "detection_method": null}} +{"question_id": "PPIL1-0007", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: HNRNPD (Q14103)\n Function: Binds with high affinity to RNA molecules that contain AU-rich elements (AREs) found within the 3'-UTR of many proto-oncogenes and cytokine mRNAs. Also binds to double- and single-stranded DNA sequenc\n Location: Nucleus\n\nProtein 2: SYNCRIP (O60506)\n Function: Heterogenous nuclear ribonucleoprotein (hnRNP) implicated in mRNA processing mechanisms. Component of the CRD-mediated complex that promotes MYC mRNA stability. Isoform 1, isoform 2 and isoform 3 are \n Location: Cytoplasm\n\nEvidence: Available evidence does not support a physical interaction between HNRNPD and SYNCRIP.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 60, "gene_symbol_1": "HNRNPD", "gene_symbol_2": "SYNCRIP", "detection_method": null}} +{"question_id": "PPIL1-0008", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: TADA2A (O75478)\n Function: Component of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4. Required for the function of some acidic activation domains, which activate transcription from a\n Location: Nucleus\n\nProtein 2: HNRNPLL (Q8WVV9)\n Function: RNA-binding protein that functions as a regulator of alternative splicing for multiple target mRNAs, including PTPRC/CD45 and STAT5A. Required for alternative splicing of PTPRC\n\nEvidence: Available evidence does not support a physical interaction between TADA2A and HNRNPLL.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 353, "gene_symbol_1": "TADA2A", "gene_symbol_2": "HNRNPLL", "detection_method": null}} +{"question_id": "PPIL1-0009", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: LDOC1 (O95751)\n Function: May have an important role in the development and/or progression of some cancers\n Location: Nucleus\n\nProtein 2: INPP1 (P49441)\n Function: Mg(2+)-dependent phosphatase that catalyzes the hydrolysis of the 1-position phosphate from inositol 1,4-bisphosphate and inositol 1,3,4-trisphosphate and participates in inositol phosphate metabolism\n\nEvidence: Available evidence does not support a physical interaction between LDOC1 and INPP1.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 342, "gene_symbol_1": "LDOC1", "gene_symbol_2": "INPP1", "detection_method": null}} +{"question_id": "PPIL1-0010", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: BCL2L1 (Q07817)\n Function: Potent inhibitor of cell death. Inhibits activation of caspases. Appears to regulate cell death by blocking the voltage-dependent anion channel (VDAC) by binding to it and preventing the release of th\n Location: Mitochondrion inner membrane\n\nProtein 2: BIK (Q13323)\n Function: Accelerates programmed cell death. Association to the apoptosis repressors Bcl-X(L), BHRF1, Bcl-2 or its adenovirus homolog E1B 19k protein suppresses this death-promoting activity. Does not interact \n Location: Endomembrane system\n\nEvidence: Available evidence does not support a physical interaction between BCL2L1 and BIK.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 203, "gene_symbol_1": "BCL2L1", "gene_symbol_2": "BIK", "detection_method": null}} +{"question_id": "PPIL1-0011", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: PRMT2 (P55345)\n Function: Arginine methyltransferase that methylates the guanidino nitrogens of arginyl residues in proteins such as STAT3, FBL, histone H4 (PubMed:19405910). Acts as a coactivator (with NCOA2) of the androgen \n Location: Cytoplasm\n\nProtein 2: RBM12 (Q9NTZ6)\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between PRMT2 and RBM12.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 526, "gene_symbol_1": "PRMT2", "gene_symbol_2": "RBM12", "detection_method": null}} +{"question_id": "PPIL1-0012", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: PRNP (P04156)\n Function: Its primary physiological function is unclear. May play a role in neuronal development and synaptic plasticity. May be required for neuronal myelin sheath maintenance. May promote myelin homeostasis t\n Location: Cell membrane\n\nProtein 2: TSC22D4 (Q9Y3Q8)\n Function: Binds DNA and acts as a transcriptional repressor (PubMed:10488076). Involved in the regulation of systematic glucose homeostasis and insulin sensitivity, via transcriptional repression of downstream \n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between PRNP and TSC22D4.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "gold", "result_id": 49, "gene_symbol_1": "PRNP", "gene_symbol_2": "TSC22D4", "detection_method": null}} +{"question_id": "PPIL1-0013", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: TRIM23 (P36406)\n Function: Acts as an E3 ubiquitin-protein ligase. Plays an essential role in autophagy activation during viral infection. Mechanistically, activates TANK-binding kinase 1/TBK1 by facilitating its dimerization a\n Location: Cytoplasm\n\nProtein 2: DNAAF4 (Q8WXU2)\n Function: Axonemal dynein assembly factor required for ciliary motility. Involved in neuronal migration during development of the cerebral neocortex. May regulate the stability and proteasomal degradation of th\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between TRIM23 and DNAAF4.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 252, "gene_symbol_1": "TRIM23", "gene_symbol_2": "DNAAF4", "detection_method": null}} +{"question_id": "PPIL1-0014", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: BANP (A0A0S2Z5G4)\n Location: Nucleus\n\nProtein 2: FOXM1 (Q08050)\n Function: Transcription factor regulating the expression of cell cycle genes essential for DNA replication and mitosis (PubMed:19160488, PubMed:20360045). Plays a role in the control of cell proliferation (PubM\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between BANP and FOXM1.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 178, "gene_symbol_1": "BANP", "gene_symbol_2": "FOXM1", "detection_method": null}} +{"question_id": "PPIL1-0015", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: BANP (A0A0S2Z5G4)\n Location: Nucleus\n\nProtein 2: FHL5 (Q5TD97)\n Function: May be involved in the regulation of spermatogenesis. Stimulates CREM transcriptional activity in a phosphorylation-independent manner\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between BANP and FHL5.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 162, "gene_symbol_1": "BANP", "gene_symbol_2": "FHL5", "detection_method": null}} +{"question_id": "PPIL1-0016", "task": "ppi-l1", "split": "fewshot", "difficulty": "hard", "context_text": "Protein 1: TRIM27 (P14373)\n Function: E3 ubiquitin-protein ligase that mediates ubiquitination of various substrates and thereby plays a role in diffent processes including proliferation, innate immunity, apoptosis, immune response or aut\n Location: Nucleus\n\nProtein 2: HNRNPLL (Q8WVV9)\n Function: RNA-binding protein that functions as a regulator of alternative splicing for multiple target mRNAs, including PTPRC/CD45 and STAT5A. Required for alternative splicing of PTPRC\n\nEvidence: Available evidence does not support a physical interaction between TRIM27 and HNRNPLL.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 312, "gene_symbol_1": "TRIM27", "gene_symbol_2": "HNRNPLL", "detection_method": null}} +{"question_id": "PPIL1-0017", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: PPP2CA (P67775)\n Function: Catalytic subunit of protein phosphatase 2A (PP2A), a serine/threonine phosphatase involved in the regulation of a wide variety of enzymes, signal transduction pathways, and cellular events (PubMed:10\n Location: Cytoplasm\n\nProtein 2: PPME1 (Q9Y570)\n Function: Demethylates proteins that have been reversibly carboxymethylated. Demethylates PPP2CB (in vitro) and PPP2CA. Binding to PPP2CA displaces the manganese ion and inactivates the enzyme\n\nEvidence: Available evidence does not support a physical interaction between PPP2CA and PPME1.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 762, "gene_symbol_1": "PPP2CA", "gene_symbol_2": "PPME1", "detection_method": null}} +{"question_id": "PPIL1-0018", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: BTC (A0A0S2Z3I5)\n\nProtein 2: GMPPA (Q96IJ6)\n Function: Regulatory subunit of the GMPPA-GMPPB mannose-1-phosphate guanylyltransferase complex; reduces the catalytic activity of GMPPB when part of the complex (PubMed:24035193, PubMed:33986552). Mediates all\n Location: Cytoplasm\n\nEvidence: Available evidence does not support a physical interaction between BTC and GMPPA.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 204, "gene_symbol_1": "BTC", "gene_symbol_2": "GMPPA", "detection_method": null}} +{"question_id": "PPIL1-0019", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: TLE6 (Q9H808)\n Function: Component of the subcortical maternal complex (SCMC), a multiprotein complex that plays a key role in early embryonic development (PubMed:26537248). The SCMC complex is a structural constituent of cyt\n Location: Cytoplasm\n\nProtein 2: KHDC3L (Q587J8)\n Function: Component of the subcortical maternal complex (SCMC), a multiprotein complex that plays a key role in early embryonic development (By similarity). The SCMC complex is a structural constituent of cytop\n Location: Cytoplasm\n\nEvidence: Available evidence does not support a physical interaction between TLE6 and KHDC3L.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 770, "gene_symbol_1": "TLE6", "gene_symbol_2": "KHDC3L", "detection_method": null}} +{"question_id": "PPIL1-0020", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: RXRB (A0A0S2Z4M5)\n Location: Nucleus\n\nProtein 2: EXOSC8 (Q96B26)\n Function: Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA \n Location: Cytoplasm\n\nEvidence: Available evidence does not support a physical interaction between RXRB and EXOSC8.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 548, "gene_symbol_1": "RXRB", "gene_symbol_2": "EXOSC8", "detection_method": null}} +{"question_id": "PPIL1-0021", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: FYN (P06241)\n Function: Non-receptor tyrosine-protein kinase that plays a role in many biological processes including regulation of cell growth and survival, cell adhesion, integrin-mediated signaling, cytoskeletal remodelin\n Location: Cytoplasm\n\nProtein 2: CBL (P22681)\n Function: E3 ubiquitin-protein ligase that acts as a negative regulator of many signaling pathways by mediating ubiquitination of cell surface receptors (PubMed:10514377, PubMed:11896602, PubMed:14661060, PubMe\n Location: Cytoplasm\n\nEvidence: Available evidence does not support a physical interaction between FYN and CBL.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "gold", "result_id": 24, "gene_symbol_1": "FYN", "gene_symbol_2": "CBL", "detection_method": null}} +{"question_id": "PPIL1-0022", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: RIN1 (Q13671)\n Function: Ras effector protein, which may serve as an inhibitory modulator of neuronal plasticity in aversive memory formation. Can affect Ras signaling at different levels. First, by competing with RAF1 protei\n Location: Cytoplasm\n\nProtein 2: LZTS2 (Q9BRK4)\n Function: Negative regulator of katanin-mediated microtubule severing and release from the centrosome. Required for central spindle formation and the completion of cytokinesis. May negatively regulate axonal ou\n Location: Cytoplasm\n\nEvidence: Available evidence does not support a physical interaction between RIN1 and LZTS2.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 512, "gene_symbol_1": "RIN1", "gene_symbol_2": "LZTS2", "detection_method": null}} +{"question_id": "PPIL1-0023", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: MEOX2 (Q6FHY5)\n Location: Nucleus\n\nProtein 2: UPP2 (A0A0S2Z698)\n\nEvidence: Available evidence does not support a physical interaction between MEOX2 and UPP2.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 617, "gene_symbol_1": "MEOX2", "gene_symbol_2": "UPP2", "detection_method": null}} +{"question_id": "PPIL1-0024", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: NCK2 (E7ERP6)\n\nProtein 2: LZTS2 (Q9BRK4)\n Function: Negative regulator of katanin-mediated microtubule severing and release from the centrosome. Required for central spindle formation and the completion of cytokinesis. May negatively regulate axonal ou\n Location: Cytoplasm\n\nEvidence: Available evidence does not support a physical interaction between NCK2 and LZTS2.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 412, "gene_symbol_1": "NCK2", "gene_symbol_2": "LZTS2", "detection_method": null}} +{"question_id": "PPIL1-0025", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: CBLB (Q13191)\n Function: E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome (PubMed:2052\n Location: Cytoplasm\n\nProtein 2: PRMT2 (P55345)\n Function: Arginine methyltransferase that methylates the guanidino nitrogens of arginyl residues in proteins such as STAT3, FBL, histone H4 (PubMed:19405910). Acts as a coactivator (with NCOA2) of the androgen \n Location: Cytoplasm\n\nEvidence: Available evidence does not support a physical interaction between CBLB and PRMT2.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 519, "gene_symbol_1": "CBLB", "gene_symbol_2": "PRMT2", "detection_method": null}} +{"question_id": "PPIL1-0026", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: NDN (A0A0S2Z442)\n\nProtein 2: SSX2IP (Q9Y2D8)\n Function: Belongs to an adhesion system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). May connect the nectin-afadin and E-cadherin-caten\n Location: Cell junction, adherens junction\n\nEvidence: Available evidence does not support a physical interaction between NDN and SSX2IP.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 425, "gene_symbol_1": "NDN", "gene_symbol_2": "SSX2IP", "detection_method": null}} +{"question_id": "PPIL1-0027", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: RNF6 (Q9Y252)\n Function: E3 ubiquitin-protein ligase mediating 'Lys-48'-linked polyubiquitination of LIMK1 and its subsequent targeting to the proteasome for degradation (By similarity). Negatively regulates axonal outgrowth \n Location: Nucleus\n\nProtein 2: MIPOL1 (Q8TD10)\n\nEvidence: Available evidence does not support a physical interaction between RNF6 and MIPOL1.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 527, "gene_symbol_1": "RNF6", "gene_symbol_2": "MIPOL1", "detection_method": null}} +{"question_id": "PPIL1-0028", "task": "ppi-l1", "split": "fewshot", "difficulty": "hard", "context_text": "Protein 1: YWHAZ (P63104)\n Function: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways (PubMed:14578935, PubMed:15071501, PubMed:15644438, PubMed:16376338, PubMed:16959763\n Location: Cytoplasm\n\nProtein 2: HNRNPD (Q14103)\n Function: Binds with high affinity to RNA molecules that contain AU-rich elements (AREs) found within the 3'-UTR of many proto-oncogenes and cytokine mRNAs. Also binds to double- and single-stranded DNA sequenc\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between YWHAZ and HNRNPD.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "gold", "result_id": 54, "gene_symbol_1": "YWHAZ", "gene_symbol_2": "HNRNPD", "detection_method": null}} +{"question_id": "PPIL1-0029", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: WASL (O00401)\n Function: Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex (PubMed:16767080, PubMed:19366662, PubMed:19487689, PubMed:22847007, PubMed:22921828, PubMed:9422512).\n Location: Cytoplasm, cytoskeleton\n\nProtein 2: PRMT2 (P55345)\n Function: Arginine methyltransferase that methylates the guanidino nitrogens of arginyl residues in proteins such as STAT3, FBL, histone H4 (PubMed:19405910). Acts as a coactivator (with NCOA2) of the androgen \n Location: Cytoplasm\n\nEvidence: Available evidence does not support a physical interaction between WASL and PRMT2.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 520, "gene_symbol_1": "WASL", "gene_symbol_2": "PRMT2", "detection_method": null}} +{"question_id": "PPIL1-0030", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: E2F6 (Q2Z196)\n\nProtein 2: TFDP2 (Q14188)\n Function: Can stimulate E2F-dependent transcription. Binds DNA cooperatively with E2F family members through the E2 recognition site, 5'-TTTC[CG]CGC-3', found in the promoter region of a number of genes whose p\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between E2F6 and TFDP2.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 274, "gene_symbol_1": "E2F6", "gene_symbol_2": "TFDP2", "detection_method": null}} +{"question_id": "PPIL1-0031", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: DNAAF4 (Q8WXU2)\n Function: Axonemal dynein assembly factor required for ciliary motility. Involved in neuronal migration during development of the cerebral neocortex. May regulate the stability and proteasomal degradation of th\n Location: Nucleus\n\nProtein 2: CRACR2B (Q8N4Y2)\n Function: Plays a role in store-operated Ca(2+) entry (SOCE)\n\nEvidence: Available evidence does not support a physical interaction between DNAAF4 and CRACR2B.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 269, "gene_symbol_1": "DNAAF4", "gene_symbol_2": "CRACR2B", "detection_method": null}} +{"question_id": "PPIL1-0032", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: COL1A2 (A0A0S2Z3K0)\n\nProtein 2: KCNIP4 (Q6PIL6)\n Function: Regulatory subunit of Kv4/D (Shal)-type voltage-gated rapidly inactivating A-type potassium channels. Modulates KCND2 channel density, inactivation kinetics and rate of recovery from inactivation in a\n Location: Cell membrane\n\nEvidence: Available evidence does not support a physical interaction between COL1A2 and KCNIP4.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 224, "gene_symbol_1": "COL1A2", "gene_symbol_2": "KCNIP4", "detection_method": null}} +{"question_id": "PPIL1-0033", "task": "ppi-l1", "split": "fewshot", "difficulty": "hard", "context_text": "Protein 1: HGS (A0A0S2Z4Q4)\n Location: Cytoplasm\n\nProtein 2: TRIM17 (Q9Y577)\n Function: E3 ubiquitin ligase that plays important roles in the regulation of neuronal apoptosis, selective autophagy or cell proliferation (PubMed:19358823, PubMed:22023800, PubMed:27562068). Stimulates the de\n Location: Cytoplasm\n\nEvidence: Available evidence does not support a physical interaction between HGS and TRIM17.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 317, "gene_symbol_1": "HGS", "gene_symbol_2": "TRIM17", "detection_method": null}} +{"question_id": "PPIL1-0034", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: CRLF3 (Q8IUI8)\n Function: May play a role in the negative regulation of cell cycle progression\n Location: Cytoplasm\n\nProtein 2: RBM14 (Q96PK6)\n Function: Isoform 1 may function as a nuclear receptor coactivator, enhancing transcription through other coactivators such as NCOA6 and CITED1. Isoform 2, functions as a transcriptional repressor, modulating t\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between CRLF3 and RBM14.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 529, "gene_symbol_1": "CRLF3", "gene_symbol_2": "RBM14", "detection_method": null}} +{"question_id": "PPIL1-0035", "task": "ppi-l1", "split": "fewshot", "difficulty": "hard", "context_text": "Protein 1: CIC (Q96RK0)\n Function: Transcriptional repressor which plays a role in development of the central nervous system (CNS). In concert with ATXN1 and ATXN1L, involved in brain development\n Location: Nucleus\n\nProtein 2: SETD2 (Q9BYW2)\n Function: Histone methyltransferase that specifically trimethylates 'Lys-36' of histone H3 (H3K36me3) using dimethylated 'Lys-36' (H3K36me2) as substrate (PubMed:16118227, PubMed:19141475, PubMed:21526191, PubM\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between CIC and SETD2.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "gold", "result_id": 53, "gene_symbol_1": "CIC", "gene_symbol_2": "SETD2", "detection_method": null}} +{"question_id": "PPIL1-0036", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: MEOX2 (Q6FHY5)\n Location: Nucleus\n\nProtein 2: UPP2 (A0A0S2Z639)\n\nEvidence: Available evidence does not support a physical interaction between MEOX2 and UPP2.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 616, "gene_symbol_1": "MEOX2", "gene_symbol_2": "UPP2", "detection_method": null}} +{"question_id": "PPIL1-0037", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: SQSTM1 (Q13501)\n Function: Molecular adapter required for selective macroautophagy (aggrephagy) by acting as a bridge between polyubiquitinated proteins and autophagosomes (PubMed:15340068, PubMed:15953362, PubMed:16286508, Pub\n Location: Cytoplasmic vesicle, autophagosome\n\nProtein 2: TRIM63 (Q969Q1)\n Function: E3 ubiquitin ligase. Mediates the ubiquitination and subsequent proteasomal degradation of CKM, GMEB1 and HIBADH. Regulates the proteasomal degradation of muscle proteins under amino acid starvation, \n Location: Cytoplasm\n\nEvidence: Available evidence does not support a physical interaction between SQSTM1 and TRIM63.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 764, "gene_symbol_1": "SQSTM1", "gene_symbol_2": "TRIM63", "detection_method": null}} +{"question_id": "PPIL1-0038", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: UBQLN4 (Q9NRR5)\n Function: Regulator of protein degradation that mediates the proteasomal targeting of misfolded, mislocalized or accumulated proteins (PubMed:15280365, PubMed:27113755, PubMed:29666234, PubMed:30612738). Acts b\n Location: Nucleus\n\nProtein 2: NOTCH2NLA (Q7Z3S9)\n Function: Human-specific protein that promotes neural progenitor proliferation and evolutionary expansion of the brain neocortex by regulating the Notch signaling pathway (PubMed:29561261, PubMed:29856954, PubM\n Location: Secreted\n\nEvidence: Available evidence does not support a physical interaction between UBQLN4 and NOTCH2NLA.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "gold", "result_id": 52, "gene_symbol_1": "UBQLN4", "gene_symbol_2": "NOTCH2NLA", "detection_method": null}} +{"question_id": "PPIL1-0039", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: AMMECR1 (Q9Y4X0)\n Location: Nucleus\n\nProtein 2: EFEMP2 (O95967)\n Function: Plays a crucial role in elastic fiber formation in tissue, and in the formation of ultrastructural connections between elastic laminae and smooth muscle cells in the aorta, therefore participates in t\n Location: Secreted, extracellular space, extracellular matrix\n\nEvidence: Available evidence does not support a physical interaction between AMMECR1 and EFEMP2.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 151, "gene_symbol_1": "AMMECR1", "gene_symbol_2": "EFEMP2", "detection_method": null}} +{"question_id": "PPIL1-0040", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: GOLGA2 (Q08379)\n Function: Peripheral membrane component of the cis-Golgi stack that acts as a membrane skeleton that maintains the structure of the Golgi apparatus, and as a vesicle thether that facilitates vesicle fusion to t\n Location: Golgi apparatus, cis-Golgi network membrane\n\nProtein 2: TEX9 (A0A0S2Z6S1)\n\nEvidence: Available evidence does not support a physical interaction between GOLGA2 and TEX9.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 612, "gene_symbol_1": "GOLGA2", "gene_symbol_2": "TEX9", "detection_method": null}} +{"question_id": "PPIL1-0041", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: CALCOCO2 (Q13137)\n Function: Xenophagy-specific receptor required for autophagy-mediated intracellular bacteria degradation. Acts as an effector protein of galectin-sensed membrane damage that restricts the proliferation of infec\n Location: Cytoplasm, perinuclear region\n\nProtein 2: RXRB (A0A0S2Z4M5)\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between CALCOCO2 and RXRB.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 545, "gene_symbol_1": "CALCOCO2", "gene_symbol_2": "RXRB", "detection_method": null}} +{"question_id": "PPIL1-0042", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: EPS8 (Q12929)\n Function: Signaling adapter that controls various cellular protrusions by regulating actin cytoskeleton dynamics and architecture. Depending on its association with other signal transducers, can regulate differ\n Location: Cytoplasm, cell cortex\n\nProtein 2: ITGB7 (P26010)\n Function: Integrin ITGA4/ITGB7 (alpha-4/beta-7) (Peyer patches-specific homing receptor LPAM-1) is an adhesion molecule that mediates lymphocyte migration and homing to gut-associated lymphoid tissue (GALT) (Pr\n Location: Cell membrane\n\nEvidence: Available evidence does not support a physical interaction between EPS8 and ITGB7.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "gold", "result_id": 11, "gene_symbol_1": "EPS8", "gene_symbol_2": "ITGB7", "detection_method": null}} +{"question_id": "PPIL1-0043", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: CD99L2 (A0A0S2Z5R8)\n Function: Plays a role in a late step of leukocyte extravasation helping cells to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Homophilic adhesion molecule\n Location: Cell junction\n\nProtein 2: SGTA (O43765)\n Function: Co-chaperone that binds misfolded and hydrophobic patches-containing client proteins in the cytosol. Mediates their targeting to the endoplasmic reticulum but also regulates their sorting to the prote\n Location: Cytoplasm\n\nEvidence: Available evidence does not support a physical interaction between CD99L2 and SGTA.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 225, "gene_symbol_1": "CD99L2", "gene_symbol_2": "SGTA", "detection_method": null}} +{"question_id": "PPIL1-0044", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: RXRB (A0A0S2Z4M5)\n Location: Nucleus\n\nProtein 2: NR1H4 (Q96RI1)\n Function: Ligand-activated transcription factor. Receptor for bile acids (BAs) such as chenodeoxycholic acid (CDCA), lithocholic acid, deoxycholic acid (DCA) and allocholic acid (ACA). Plays a essential role in\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between RXRB and NR1H4.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 553, "gene_symbol_1": "RXRB", "gene_symbol_2": "NR1H4", "detection_method": null}} +{"question_id": "PPIL1-0045", "task": "ppi-l1", "split": "fewshot", "difficulty": "hard", "context_text": "Protein 1: MDFI (Q99750)\n Function: Inhibits the transactivation activity of the Myod family of myogenic factors and represses myogenesis (By similarity). Acts by associating with Myod family members and retaining them in the cytoplasm \n Location: Nucleus\n\nProtein 2: TEAD4 (Q15561)\n Function: Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The cor\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between MDFI and TEAD4.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 607, "gene_symbol_1": "MDFI", "gene_symbol_2": "TEAD4", "detection_method": null}} +{"question_id": "PPIL1-0046", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: AGTRAP (Q6RW13)\n Function: Appears to be a negative regulator of type-1 angiotensin II receptor-mediated signaling by regulating receptor internalization as well as mechanism of receptor desensitization such as phosphorylation.\n Location: Endoplasmic reticulum membrane\n\nProtein 2: FGA (A0A0S2Z3E8)\n Function: Cleaved by the protease thrombin to yield monomers which, together with fibrinogen beta (FGB) and fibrinogen gamma (FGG), polymerize to form an insoluble fibrin matrix. Fibrin has a major function in \n Location: Secreted\n\nEvidence: Available evidence does not support a physical interaction between AGTRAP and FGA.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 287, "gene_symbol_1": "AGTRAP", "gene_symbol_2": "FGA", "detection_method": null}} +{"question_id": "PPIL1-0047", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: NDN (A0A0S2Z442)\n\nProtein 2: APBB1IP (Q7Z5R6)\n Function: Appears to function in the signal transduction from Ras activation to actin cytoskeletal remodeling. Suppresses insulin-induced promoter activities through AP1 and SRE. Mediates Rap1-induced adhesion\n Location: Cell membrane\n\nEvidence: Available evidence does not support a physical interaction between NDN and APBB1IP.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 484, "gene_symbol_1": "NDN", "gene_symbol_2": "APBB1IP", "detection_method": null}} +{"question_id": "PPIL1-0048", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: CRK (P46108)\n Function: Involved in cell branching and adhesion mediated by BCAR1-CRK-RAPGEF1 signaling and activation of RAP1\n Location: Cytoplasm\n\nProtein 2: PPFIBP2 (Q8ND30)\n Function: May regulate the disassembly of focal adhesions. Did not bind receptor-like tyrosine phosphatases type 2A\n\nEvidence: Available evidence does not support a physical interaction between CRK and PPFIBP2.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 220, "gene_symbol_1": "CRK", "gene_symbol_2": "PPFIBP2", "detection_method": null}} +{"question_id": "PPIL1-0049", "task": "ppi-l1", "split": "fewshot", "difficulty": "hard", "context_text": "Protein 1: CLCN2 (A0A0S2Z3G7)\n Location: Membrane\n\nProtein 2: FHL3 (Q13643)\n Function: Recruited by SOX15 to FOXK1 promoters where it acts as a transcriptional coactivator of FOXK1\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between CLCN2 and FHL3.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 215, "gene_symbol_1": "CLCN2", "gene_symbol_2": "FHL3", "detection_method": null}} +{"question_id": "PPIL1-0050", "task": "ppi-l1", "split": "fewshot", "difficulty": "hard", "context_text": "Protein 1: KRT13 (P13646)\n Function: Type 1 keratin (Probable). Maintains postnatal tongue mucosal cell homeostasis and tissue organization in response to mechanical stress, potentially via regulation of the G1/S phase cyclins CCNE1 and \n\nProtein 2: DYX1C1 (A0A0S2Z5X9)\n Location: Cell projection, neuron projection\n\nEvidence: Available evidence does not support a physical interaction between KRT13 and DYX1C1.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 267, "gene_symbol_1": "KRT13", "gene_symbol_2": "DYX1C1", "detection_method": null}} +{"question_id": "PPIL1-0051", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: CXCR3 (P49682)\n Function: Receptor for the C-X-C chemokine CXCL9, CXCL10 and CXCL11 and mediates the proliferation, survival and angiogenic activity of human mesangial cells (HMC) through a heterotrimeric G-protein signaling p\n Location: Cell membrane\n\nProtein 2: FATE1 (Q969F0)\n Function: Involved in the regulation of endoplasmic reticulum (ER)-mitochondria coupling. Negatively regulates the ER-mitochondria distance and Ca(2+) transfer from ER to mitochondria possibly implicating it in\n Location: Mitochondrion\n\nEvidence: Available evidence does not support a physical interaction between CXCR3 and FATE1.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 209, "gene_symbol_1": "CXCR3", "gene_symbol_2": "FATE1", "detection_method": null}} +{"question_id": "PPIL1-0052", "task": "ppi-l1", "split": "fewshot", "difficulty": "hard", "context_text": "Protein 1: ACBD4 (A0A0S2Z5W7)\n\nProtein 2: CRX (O43186)\n Function: Transcription factor that binds and transactivates the sequence 5'-TAATC[CA]-3' which is found upstream of several photoreceptor-specific genes, including the opsin genes. Acts synergistically with ot\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between ACBD4 and CRX.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 129, "gene_symbol_1": "ACBD4", "gene_symbol_2": "CRX", "detection_method": null}} +{"question_id": "PPIL1-0053", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: BTRC (B7Z3H4)\n\nProtein 2: RASSF5 (Q8WWW0)\n Function: Potential tumor suppressor. Seems to be involved in lymphocyte adhesion by linking RAP1A activation upon T-cell receptor or chemokine stimulation to integrin activation. Isoform 2 stimulates lymphocyt\n Location: Cytoplasm\n\nEvidence: Available evidence does not support a physical interaction between BTRC and RASSF5.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 202, "gene_symbol_1": "BTRC", "gene_symbol_2": "RASSF5", "detection_method": null}} +{"question_id": "PPIL1-0054", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: HNRNPLL (Q8WVV9)\n Function: RNA-binding protein that functions as a regulator of alternative splicing for multiple target mRNAs, including PTPRC/CD45 and STAT5A. Required for alternative splicing of PTPRC\n\nProtein 2: CALCOCO2 (Q13137)\n Function: Xenophagy-specific receptor required for autophagy-mediated intracellular bacteria degradation. Acts as an effector protein of galectin-sensed membrane damage that restricts the proliferation of infec\n Location: Cytoplasm, perinuclear region\n\nEvidence: Available evidence does not support a physical interaction between HNRNPLL and CALCOCO2.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 352, "gene_symbol_1": "HNRNPLL", "gene_symbol_2": "CALCOCO2", "detection_method": null}} +{"question_id": "PPIL1-0055", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: TRAF1 (Q13077)\n Function: Adapter molecule that regulates the activation of NF-kappa-B and JNK. Plays a role in the regulation of cell survival and apoptosis. The heterotrimer formed by TRAF1 and TRAF2 is part of a E3 ubiquiti\n\nProtein 2: TEAD4 (A0A0S2Z4E5)\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between TRAF1 and TEAD4.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 573, "gene_symbol_1": "TRAF1", "gene_symbol_2": "TEAD4", "detection_method": null}} +{"question_id": "PPIL1-0056", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: SIAH1 (Q8IUQ4)\n Function: E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins (PubMed:14506261, PubMed:14645235, PubMed:14654780, PubMed:15064394, PubMed:16085652,\n Location: Cytoplasm\n\nProtein 2: RXRB (P28702)\n Function: Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression \n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between SIAH1 and RXRB.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 540, "gene_symbol_1": "SIAH1", "gene_symbol_2": "RXRB", "detection_method": null}} +{"question_id": "PPIL1-0057", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: BANP (Q8N9N5)\n Function: Controls V(D)J recombination during T-cell development by repressing T-cell receptor (TCR) beta enhancer function (By similarity). Binds to scaffold/matrix attachment region beta (S/MARbeta), an ATC-r\n Location: Nucleus\n\nProtein 2: PHF21B (Q96EK2)\n\nEvidence: Available evidence does not support a physical interaction between BANP and PHF21B.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 500, "gene_symbol_1": "BANP", "gene_symbol_2": "PHF21B", "detection_method": null}} +{"question_id": "PPIL1-0058", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: AXIN1 (O15169)\n Function: Component of the beta-catenin destruction complex required for regulating CTNNB1 levels through phosphorylation and ubiquitination, and modulating Wnt-signaling (PubMed:12192039, PubMed:27098453, PubM\n Location: Cytoplasm\n\nProtein 2: NBR1 (Q14596)\n Function: Ubiquitin-binding autophagy adapter that participates in different processes including host defense or intracellular homeostasis (PubMed:24692539, PubMed:33577621). Possesses a double function during \n Location: Cytoplasm\n\nEvidence: Available evidence does not support a physical interaction between AXIN1 and NBR1.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 147, "gene_symbol_1": "AXIN1", "gene_symbol_2": "NBR1", "detection_method": null}} +{"question_id": "PPIL1-0059", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: NLRP5 (P59047)\n Function: Component of the subcortical maternal complex (SCMC), a multiprotein complex that plays a key role in early embryonic development. The SCMC complex is a structural constituent of cytoplasmic lattices,\n Location: Cytoplasm\n\nProtein 2: TLE6 (Q9H808)\n Function: Component of the subcortical maternal complex (SCMC), a multiprotein complex that plays a key role in early embryonic development (PubMed:26537248). The SCMC complex is a structural constituent of cyt\n Location: Cytoplasm\n\nEvidence: Available evidence does not support a physical interaction between NLRP5 and TLE6.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 768, "gene_symbol_1": "NLRP5", "gene_symbol_2": "TLE6", "detection_method": null}} +{"question_id": "PPIL1-0060", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: PIMREG (Q9BSJ6)\n Function: During mitosis, may play a role in the control of metaphase-to-anaphase transition\n Location: Nucleus\n\nProtein 2: CCNDBP1 (O95273)\n Function: May negatively regulate cell cycle progression. May act at least in part via inhibition of the cyclin-D1/CDK4 complex, thereby preventing phosphorylation of RB1 and blocking E2F-dependent transcriptio\n Location: Cytoplasm\n\nEvidence: Available evidence does not support a physical interaction between PIMREG and CCNDBP1.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 275, "gene_symbol_1": "PIMREG", "gene_symbol_2": "CCNDBP1", "detection_method": null}} +{"question_id": "PPIL1-0061", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: DOLK (A0A0S2Z5Y1)\n Location: Endoplasmic reticulum membrane\n\nProtein 2: KCNA3 (P22001)\n Function: Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a\n Location: Cell membrane\n\nEvidence: Available evidence does not support a physical interaction between DOLK and KCNA3.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 239, "gene_symbol_1": "DOLK", "gene_symbol_2": "KCNA3", "detection_method": null}} +{"question_id": "PPIL1-0062", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: ZNF688 (A0A0S2Z6P0)\n\nProtein 2: PCBD1 (P61457)\n Function: Involved in tetrahydrobiopterin biosynthesis (By similarity). Seems to both prevent the formation of 7-pterins and accelerate the formation of quinonoid-BH2. Coactivator for HNF1A-dependent transcript\n Location: Cytoplasm\n\nEvidence: Available evidence does not support a physical interaction between ZNF688 and PCBD1.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 731, "gene_symbol_1": "ZNF688", "gene_symbol_2": "PCBD1", "detection_method": null}} +{"question_id": "PPIL1-0063", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: PLK4 (O00444)\n Function: Serine/threonine-protein kinase that plays a central role in centriole duplication. Able to trigger procentriole formation on the surface of the parental centriole cylinder, leading to the recruitment\n Location: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole\n\nProtein 2: BTRC (A0A0S2Z4Q5)\n\nEvidence: Available evidence does not support a physical interaction between PLK4 and BTRC.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 191, "gene_symbol_1": "PLK4", "gene_symbol_2": "BTRC", "detection_method": null}} +{"question_id": "PPIL1-0064", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: REL (Q04864)\n Function: Proto-oncogene that may play a role in differentiation and lymphopoiesis. NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological \n Location: Nucleus\n\nProtein 2: ACBD4 (Q8NC06)\n Function: Binds medium- and long-chain acyl-CoA esters and may function as an intracellular carrier of acyl-CoA esters\n\nEvidence: Available evidence does not support a physical interaction between REL and ACBD4.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 132, "gene_symbol_1": "REL", "gene_symbol_2": "ACBD4", "detection_method": null}} +{"question_id": "PPIL1-0065", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: BTRC (A0A0S2Z4Q5)\n\nProtein 2: TRIM9 (Q9C026)\n Function: E3 ubiquitin-protein ligase which ubiquitinates itself in cooperation with an E2 enzyme UBE2D2/UBC4 and serves as a targeting signal for proteasomal degradation. May play a role in regulation of neuro\n Location: Cytoplasm\n\nEvidence: Available evidence does not support a physical interaction between BTRC and TRIM9.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 197, "gene_symbol_1": "BTRC", "gene_symbol_2": "TRIM9", "detection_method": null}} +{"question_id": "PPIL1-0066", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: PNMA1 (Q8ND90)\n Location: Nucleus, nucleolus\n\nProtein 2: ZNF688 (A0A0S2Z6P0)\n\nEvidence: Available evidence does not support a physical interaction between PNMA1 and ZNF688.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 634, "gene_symbol_1": "PNMA1", "gene_symbol_2": "ZNF688", "detection_method": null}} +{"question_id": "PPIL1-0067", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: ABCF3 (Q9NUQ8)\n Function: Displays an antiviral effect against flaviviruses such as west Nile virus (WNV) in the presence of OAS1B\n\nProtein 2: THAP1 (Q9NVV9)\n Function: DNA-binding transcription regulator that regulates endothelial cell proliferation and G1/S cell-cycle progression. Specifically binds the 5'-[AT]NTNN[GT]GGCA[AGT]-3' core DNA sequence and acts by modu\n Location: Nucleus, nucleoplasm\n\nEvidence: Available evidence does not support a physical interaction between ABCF3 and THAP1.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 118, "gene_symbol_1": "ABCF3", "gene_symbol_2": "THAP1", "detection_method": null}} +{"question_id": "PPIL1-0068", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: APBA1 (Q02410)\n Function: Putative function in synaptic vesicle exocytosis by binding to Munc18-1, an essential component of the synaptic vesicle exocytotic machinery. May modulate processing of the amyloid-beta precursor prot\n Location: Cytoplasm\n\nProtein 2: RAB6B (Q9NRW1)\n Function: The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between active GTP-bound and inactiv\n Location: Golgi apparatus membrane\n\nEvidence: Available evidence does not support a physical interaction between APBA1 and RAB6B.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "gold", "result_id": 103, "gene_symbol_1": "APBA1", "gene_symbol_2": "RAB6B", "detection_method": null}} +{"question_id": "PPIL1-0069", "task": "ppi-l1", "split": "val", "difficulty": "hard", "context_text": "Protein 1: TFCP2 (Q12800)\n Function: Binds a variety of cellular and viral promoters including fibrinogen, alpha-globin, SV40 and HIV-1 promoters. Activation of the alpha-globin promoter in erythroid cells is via synergistic interaction \n Location: Nucleus\n\nProtein 2: PHF21B (Q96EK2)\n\nEvidence: Available evidence does not support a physical interaction between TFCP2 and PHF21B.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 507, "gene_symbol_1": "TFCP2", "gene_symbol_2": "PHF21B", "detection_method": null}} +{"question_id": "PPIL1-0070", "task": "ppi-l1", "split": "val", "difficulty": "hard", "context_text": "Protein 1: ZKSCAN4 (Q969J2)\n Function: May be involved in the transcriptional activation of MDM2 and EP300 genes\n Location: Nucleus\n\nProtein 2: MDM4 (O15151)\n Function: Contributes to p53/TP53 regulation (PubMed:32300648). Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding their transcriptional activation domains. Inhibits degradation\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between ZKSCAN4 and MDM4.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 637, "gene_symbol_1": "ZKSCAN4", "gene_symbol_2": "MDM4", "detection_method": null}} +{"question_id": "PPIL1-0071", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: CEP70 (Q8NHQ1)\n Function: Plays a role in the organization of both preexisting and nascent microtubules in interphase cells. During mitosis, required for the organization and orientation of the mitotic spindle\n Location: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome\n\nProtein 2: RNF6 (Q9Y252)\n Function: E3 ubiquitin-protein ligase mediating 'Lys-48'-linked polyubiquitination of LIMK1 and its subsequent targeting to the proteasome for degradation (By similarity). Negatively regulates axonal outgrowth \n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between CEP70 and RNF6.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 517, "gene_symbol_1": "CEP70", "gene_symbol_2": "RNF6", "detection_method": null}} +{"question_id": "PPIL1-0072", "task": "ppi-l1", "split": "val", "difficulty": "hard", "context_text": "Protein 1: TEAD4 (A0A0S2Z4F2)\n Location: Nucleus\n\nProtein 2: HOMEZ (Q8IX15)\n Function: May function as a transcriptional regulator\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between TEAD4 and HOMEZ.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 593, "gene_symbol_1": "TEAD4", "gene_symbol_2": "HOMEZ", "detection_method": null}} +{"question_id": "PPIL1-0073", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: RAF1 (P04049)\n Function: Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining\n Location: Cytoplasm\n\nProtein 2: KRT18 (P05783)\n Function: Required for the formation of KRT8/KRT18 filaments that are involved in ARHGEF40-mediated actin stress fiber formation and tensional force-induced stress fiber formation and reinforcement (PubMed:2682\n Location: Nucleus matrix\n\nEvidence: Available evidence does not support a physical interaction between RAF1 and KRT18.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 30, "gene_symbol_1": "RAF1", "gene_symbol_2": "KRT18", "detection_method": null}} +{"question_id": "PPIL1-0074", "task": "ppi-l1", "split": "val", "difficulty": "hard", "context_text": "Protein 1: HNRNPLL (Q8WVV9)\n Function: RNA-binding protein that functions as a regulator of alternative splicing for multiple target mRNAs, including PTPRC/CD45 and STAT5A. Required for alternative splicing of PTPRC\n\nProtein 2: BHLHE40 (O14503)\n Function: Transcriptional repressor involved in the regulation of the circadian rhythm by negatively regulating the activity of the clock genes and clock-controlled genes (PubMed:12397359, PubMed:18411297). Act\n Location: Cytoplasm\n\nEvidence: Available evidence does not support a physical interaction between HNRNPLL and BHLHE40.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 324, "gene_symbol_1": "HNRNPLL", "gene_symbol_2": "BHLHE40", "detection_method": null}} +{"question_id": "PPIL1-0075", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: FHL3 (Q13643)\n Function: Recruited by SOX15 to FOXK1 promoters where it acts as a transcriptional coactivator of FOXK1\n Location: Nucleus\n\nProtein 2: CLCN2 (A0A0S2Z3J3)\n Location: Membrane\n\nEvidence: Available evidence does not support a physical interaction between FHL3 and CLCN2.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 216, "gene_symbol_1": "FHL3", "gene_symbol_2": "CLCN2", "detection_method": null}} +{"question_id": "PPIL1-0076", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: BANP (E9PJI6)\n\nProtein 2: NMNAT1 (Q9HAN9)\n Function: Catalyzes the formation of NAD(+) from nicotinamide mononucleotide (NMN) and ATP (PubMed:17402747). Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate with the same ef\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between BANP and NMNAT1.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 175, "gene_symbol_1": "BANP", "gene_symbol_2": "NMNAT1", "detection_method": null}} +{"question_id": "PPIL1-0077", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: TRIM27 (P14373)\n Function: E3 ubiquitin-protein ligase that mediates ubiquitination of various substrates and thereby plays a role in diffent processes including proliferation, innate immunity, apoptosis, immune response or aut\n Location: Nucleus\n\nProtein 2: STK25 (O00506)\n Function: Oxidant stress-activated serine/threonine kinase that may play a role in the response to environmental stress. Targets to the Golgi apparatus where it appears to regulate protein transport events, cel\n Location: Cytoplasm\n\nEvidence: Available evidence does not support a physical interaction between TRIM27 and STK25.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 606, "gene_symbol_1": "TRIM27", "gene_symbol_2": "STK25", "detection_method": null}} +{"question_id": "PPIL1-0078", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: VIPAS39 (Q9H9C1)\n Function: Proposed to be involved in endosomal maturation implicating in part VPS33B. In epithelial cells, the VPS33B:VIPAS39 complex may play a role in the apical RAB11A-dependent recycling pathway and in the \n Location: Cytoplasm\n\nProtein 2: VPS33B (A0A0S2Z589)\n\nEvidence: Available evidence does not support a physical interaction between VIPAS39 and VPS33B.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 611, "gene_symbol_1": "VIPAS39", "gene_symbol_2": "VPS33B", "detection_method": null}} +{"question_id": "PPIL1-0079", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: NEFL (I6L9F6)\n Function: Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal interm\n Location: Cytoplasm\n\nProtein 2: GORASP1 (Q9BQQ3)\n Function: Key structural protein of the Golgi apparatus (PubMed:33301566). The membrane cisternae of the Golgi apparatus adhere to each other to form stacks, which are aligned side by side to form the Golgi rib\n Location: Golgi apparatus, cis-Golgi network membrane\n\nEvidence: Available evidence does not support a physical interaction between NEFL and GORASP1.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 473, "gene_symbol_1": "NEFL", "gene_symbol_2": "GORASP1", "detection_method": null}} +{"question_id": "PPIL1-0080", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: TLE6 (Q9H808)\n Function: Component of the subcortical maternal complex (SCMC), a multiprotein complex that plays a key role in early embryonic development (PubMed:26537248). The SCMC complex is a structural constituent of cyt\n Location: Cytoplasm\n\nProtein 2: OOEP (A6NGQ2)\n Function: Component of the subcortical maternal complex (SCMC), a multiprotein complex that plays a key role in early embryonic development. The SCMC complex is a structural constituent of cytoplasmic lattices,\n Location: Cytoplasm\n\nEvidence: Available evidence does not support a physical interaction between TLE6 and OOEP.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 769, "gene_symbol_1": "TLE6", "gene_symbol_2": "OOEP", "detection_method": null}} +{"question_id": "PPIL1-0081", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: MAGEA11 (P43364)\n Function: Acts as androgen receptor coregulator that increases androgen receptor activity by modulating the receptors interdomain interaction. May play a role in embryonal development and tumor transformation o\n Location: Nucleus\n\nProtein 2: LMBR1L (Q6UX01)\n Function: Plays an essential role in lymphocyte development by negatively regulating the canonical Wnt signaling pathway (By similarity). In association with UBAC2 and E3 ubiquitin-protein ligase AMFR, promotes\n Location: Cell membrane\n\nEvidence: Available evidence does not support a physical interaction between MAGEA11 and LMBR1L.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 381, "gene_symbol_1": "MAGEA11", "gene_symbol_2": "LMBR1L", "detection_method": null}} +{"question_id": "PPIL1-0082", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: DTNBP1 (Q96EV8)\n Function: Component of the BLOC-1 complex, a complex that is required for normal biogenesis of lysosome-related organelles (LRO), such as platelet dense granules and melanosomes. In concert with the AP-3 comple\n Location: Cytoplasm\n\nProtein 2: XRCC6 (P12956)\n Function: DNA-binding protein critical for the DNA damage response, specifically in repairing double-strand breaks (DSBs) via the classical non-homologous end joining (NHEJ) pathway. It forms a heterodimer with\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between DTNBP1 and XRCC6.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 759, "gene_symbol_1": "DTNBP1", "gene_symbol_2": "XRCC6", "detection_method": null}} +{"question_id": "PPIL1-0083", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: TP73 (O15350)\n Function: Participates in the apoptotic response to DNA damage. Isoforms containing the transactivation domain are pro-apoptotic, isoforms lacking the domain are anti-apoptotic and block the function of p53 and\n Location: Nucleus\n\nProtein 2: WWOX (Q9NZC7)\n Function: Putative oxidoreductase. Acts as a tumor suppressor and plays a role in apoptosis. Required for normal bone development (By similarity). May function synergistically with p53/TP53 to control genotoxic\n Location: Cytoplasm\n\nEvidence: Available evidence does not support a physical interaction between TP73 and WWOX.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 37, "gene_symbol_1": "TP73", "gene_symbol_2": "WWOX", "detection_method": null}} +{"question_id": "PPIL1-0084", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: RHOU (Q7L0Q8)\n Function: Binds to and activates protein kinase PAK1 (PubMed:11459829). Plays a role in the regulation of cell morphology, cytoskeletal organization and focal adhesion assembly during cell migration (PubMed:114\n Location: Cell membrane\n\nProtein 2: PLCG1 (P19174)\n Function: Mediates the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). Plays an important role in the regulation of intracellular signaling cascades. Be\n Location: Cell projection, lamellipodium\n\nEvidence: Available evidence does not support a physical interaction between RHOU and PLCG1.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 27, "gene_symbol_1": "RHOU", "gene_symbol_2": "PLCG1", "detection_method": null}} +{"question_id": "PPIL1-0085", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: KRTAP5-9 (P26371)\n Function: In the hair cortex, hair keratin intermediate filaments are embedded in an interfilamentous matrix, consisting of hair keratin-associated protein (KRTAP), which are essential for the formation of a ri\n\nProtein 2: SDC3 (A0A9K3Y886)\n Function: Cell surface proteoglycan\n Location: Membrane\n\nEvidence: Available evidence does not support a physical interaction between KRTAP5-9 and SDC3.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 570, "gene_symbol_1": "KRTAP5-9", "gene_symbol_2": "SDC3", "detection_method": null}} +{"question_id": "PPIL1-0086", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: DTX2 (Q86UW9)\n Function: Regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. Probably acts both as a positive and negative regula\n Location: Cytoplasm\n\nProtein 2: UBQLN4 (Q9NRR5)\n Function: Regulator of protein degradation that mediates the proteasomal targeting of misfolded, mislocalized or accumulated proteins (PubMed:15280365, PubMed:27113755, PubMed:29666234, PubMed:30612738). Acts b\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between DTX2 and UBQLN4.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "gold", "result_id": 44, "gene_symbol_1": "DTX2", "gene_symbol_2": "UBQLN4", "detection_method": null}} +{"question_id": "PPIL1-0087", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: CLCN2 (A0A0S2Z368)\n Location: Membrane\n\nProtein 2: FHL3 (Q13643)\n Function: Recruited by SOX15 to FOXK1 promoters where it acts as a transcriptional coactivator of FOXK1\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between CLCN2 and FHL3.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 222, "gene_symbol_1": "CLCN2", "gene_symbol_2": "FHL3", "detection_method": null}} +{"question_id": "PPIL1-0088", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: PNMA1 (Q8ND90)\n Location: Nucleus, nucleolus\n\nProtein 2: TEAD4 (A0A0S2Z4E5)\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between PNMA1 and TEAD4.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 572, "gene_symbol_1": "PNMA1", "gene_symbol_2": "TEAD4", "detection_method": null}} +{"question_id": "PPIL1-0089", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: GOLGA2 (Q08379)\n Function: Peripheral membrane component of the cis-Golgi stack that acts as a membrane skeleton that maintains the structure of the Golgi apparatus, and as a vesicle thether that facilitates vesicle fusion to t\n Location: Golgi apparatus, cis-Golgi network membrane\n\nProtein 2: DCTN4 (A0A0S2Z5D5)\n Location: Cytoplasm, cell cortex\n\nEvidence: Available evidence does not support a physical interaction between GOLGA2 and DCTN4.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 232, "gene_symbol_1": "GOLGA2", "gene_symbol_2": "DCTN4", "detection_method": null}} +{"question_id": "PPIL1-0090", "task": "ppi-l1", "split": "val", "difficulty": "hard", "context_text": "Protein 1: GMPPA (Q96IJ6)\n Function: Regulatory subunit of the GMPPA-GMPPB mannose-1-phosphate guanylyltransferase complex; reduces the catalytic activity of GMPPB when part of the complex (PubMed:24035193, PubMed:33986552). Mediates all\n Location: Cytoplasm\n\nProtein 2: NCK1 (A0A0S2Z4E4)\n\nEvidence: Available evidence does not support a physical interaction between GMPPA and NCK1.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 417, "gene_symbol_1": "GMPPA", "gene_symbol_2": "NCK1", "detection_method": null}} +{"question_id": "PPIL1-0091", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: SCHIP1 (P0DPB3)\n Location: Cytoplasm\n\nProtein 2: NF2 (P35240)\n Function: Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with\n Location: Cell projection, filopodium membrane\n\nEvidence: Available evidence does not support a physical interaction between SCHIP1 and NF2.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 67, "gene_symbol_1": "SCHIP1", "gene_symbol_2": "NF2", "detection_method": null}} +{"question_id": "PPIL1-0092", "task": "ppi-l1", "split": "val", "difficulty": "hard", "context_text": "Protein 1: BANP (A0A0S2Z5G4)\n Location: Nucleus\n\nProtein 2: FOXM1 (Q08050)\n Function: Transcription factor regulating the expression of cell cycle genes essential for DNA replication and mitosis (PubMed:19160488, PubMed:20360045). Plays a role in the control of cell proliferation (PubM\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between BANP and FOXM1.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 159, "gene_symbol_1": "BANP", "gene_symbol_2": "FOXM1", "detection_method": null}} +{"question_id": "PPIL1-0093", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: TRIP6 (Q15654)\n Function: Relays signals from the cell surface to the nucleus to weaken adherens junction and promote actin cytoskeleton reorganization and cell invasiveness. Involved in lysophosphatidic acid-induced cell adhe\n Location: Cytoplasm, cytoskeleton\n\nProtein 2: ZNF688 (A0A0S2Z6P0)\n\nEvidence: Available evidence does not support a physical interaction between TRIP6 and ZNF688.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 696, "gene_symbol_1": "TRIP6", "gene_symbol_2": "ZNF688", "detection_method": null}} +{"question_id": "PPIL1-0094", "task": "ppi-l1", "split": "val", "difficulty": "hard", "context_text": "Protein 1: CFP (P27918)\n Function: A positive regulator of the alternate pathway (AP) of complement (PubMed:16301317, PubMed:20382442, PubMed:28264884, PubMed:9748277). It binds to and stabilizes the C3- and C5-convertase enzyme comple\n Location: Secreted\n\nProtein 2: TRIP13 (Q15645)\n Function: Plays a key role in chromosome recombination and chromosome structure development during meiosis. Required at early steps in meiotic recombination that leads to non-crossovers pathways. Also needed fo\n\nEvidence: Available evidence does not support a physical interaction between CFP and TRIP13.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 207, "gene_symbol_1": "CFP", "gene_symbol_2": "TRIP13", "detection_method": null}} +{"question_id": "PPIL1-0095", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: UBQLN1 (Q9UMX0)\n Function: Plays an important role in the regulation of different protein degradation mechanisms and pathways including ubiquitin-proteasome system (UPS), autophagy and endoplasmic reticulum-associated protein d\n Location: Cytoplasm\n\nProtein 2: DOLK (Q9UPQ8)\n Function: Catalyzes CTP-mediated phosphorylation of dolichol, the terminal step in de novo dolichyl monophosphate (Dol-P) biosynthesis (PubMed:12213788, PubMed:16923818, PubMed:17273964, PubMed:22242004). Dol-P\n Location: Endoplasmic reticulum membrane\n\nEvidence: Available evidence does not support a physical interaction between UBQLN1 and DOLK.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 241, "gene_symbol_1": "UBQLN1", "gene_symbol_2": "DOLK", "detection_method": null}} +{"question_id": "PPIL1-0096", "task": "ppi-l1", "split": "val", "difficulty": "hard", "context_text": "Protein 1: KRT40 (Q6A162)\n Function: May play a role in late hair differentiation\n\nProtein 2: MXI1 (A0A0S2Z429)\n\nEvidence: Available evidence does not support a physical interaction between KRT40 and MXI1.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 422, "gene_symbol_1": "KRT40", "gene_symbol_2": "MXI1", "detection_method": null}} +{"question_id": "PPIL1-0097", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: HSPB1 (P04792)\n Function: Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state (PubMed:10383393, PubMed:20178975). Plays a role in stress resist\n Location: Cytoplasm\n\nProtein 2: VCP (P55072)\n Function: Necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. Involved in the formation of the transitional endoplasmic reticulum (tER). The transfer of membra\n Location: Cytoplasm, cytosol\n\nEvidence: Available evidence does not support a physical interaction between HSPB1 and VCP.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "gold", "result_id": 777, "gene_symbol_1": "HSPB1", "gene_symbol_2": "VCP", "detection_method": null}} +{"question_id": "PPIL1-0098", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: BCL2L1 (Q07817)\n Function: Potent inhibitor of cell death. Inhibits activation of caspases. Appears to regulate cell death by blocking the voltage-dependent anion channel (VDAC) by binding to it and preventing the release of th\n Location: Mitochondrion inner membrane\n\nProtein 2: BMF (Q96LC9)\n Function: May play a role in apoptosis. Isoform 1 seems to be the main initiator\n\nEvidence: Available evidence does not support a physical interaction between BCL2L1 and BMF.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 187, "gene_symbol_1": "BCL2L1", "gene_symbol_2": "BMF", "detection_method": null}} +{"question_id": "PPIL1-0099", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: VAC14 (Q08AM6)\n Function: Scaffold protein component of the PI(3,5)P2 regulatory complex which regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Pentamerizes into a star-shaped\n Location: Endosome membrane\n\nProtein 2: HNRPLL (A0A0S2Z5U3)\n\nEvidence: Available evidence does not support a physical interaction between VAC14 and HNRPLL.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 330, "gene_symbol_1": "VAC14", "gene_symbol_2": "HNRPLL", "detection_method": null}} +{"question_id": "PPIL1-0100", "task": "ppi-l1", "split": "val", "difficulty": "hard", "context_text": "Protein 1: GJA1 (P17302)\n Function: Structural component of the gap junction, a specialized intercellular structure consisting of a cluster of closely packed pairs of transmembrane channels, the connexons, that allow passage of small mo\n Location: Cell membrane\n\nProtein 2: DSC2 (Q02487)\n Function: A component of desmosome cell-cell junctions which are required for positive regulation of cellular adhesion (PubMed:33596089). Promotes timely incorporation of DSG2 into desmosome intercellular junct\n Location: Cell membrane\n\nEvidence: Available evidence does not support a physical interaction between GJA1 and DSC2.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "gold", "result_id": 89, "gene_symbol_1": "GJA1", "gene_symbol_2": "DSC2", "detection_method": null}} +{"question_id": "PPIL1-0101", "task": "ppi-l1", "split": "val", "difficulty": "hard", "context_text": "Protein 1: KRT31 (Q15323)\n\nProtein 2: HNRNPLL (Q8WVV9)\n Function: RNA-binding protein that functions as a regulator of alternative splicing for multiple target mRNAs, including PTPRC/CD45 and STAT5A. Required for alternative splicing of PTPRC\n\nEvidence: Available evidence does not support a physical interaction between KRT31 and HNRNPLL.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 357, "gene_symbol_1": "KRT31", "gene_symbol_2": "HNRNPLL", "detection_method": null}} +{"question_id": "PPIL1-0102", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: BANP (B4DE54)\n Location: Nucleus\n\nProtein 2: CDX4 (O14627)\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between BANP and CDX4.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 185, "gene_symbol_1": "BANP", "gene_symbol_2": "CDX4", "detection_method": null}} +{"question_id": "PPIL1-0103", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: EXOC5 (O00471)\n Function: Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane\n Location: Cytoplasm\n\nProtein 2: DNAAF4 (Q8WXU2)\n Function: Axonemal dynein assembly factor required for ciliary motility. Involved in neuronal migration during development of the cerebral neocortex. May regulate the stability and proteasomal degradation of th\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between EXOC5 and DNAAF4.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 238, "gene_symbol_1": "EXOC5", "gene_symbol_2": "DNAAF4", "detection_method": null}} +{"question_id": "PPIL1-0104", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: ZNF688 (P0C7X2)\n Function: May be involved in transcriptional regulation\n Location: Nucleus\n\nProtein 2: CEP72 (Q9P209)\n Function: Involved in the recruitment of key centrosomal proteins to the centrosome. Provides centrosomal microtubule-nucleation activity on the gamma-tubulin ring complexes (gamma-TuRCs) and has critical roles\n Location: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome\n\nEvidence: Available evidence does not support a physical interaction between ZNF688 and CEP72.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 702, "gene_symbol_1": "ZNF688", "gene_symbol_2": "CEP72", "detection_method": null}} +{"question_id": "PPIL1-0105", "task": "ppi-l1", "split": "val", "difficulty": "hard", "context_text": "Protein 1: HGS (O14964)\n Function: Involved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM, it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. Could be a direct ef\n Location: Cytoplasm\n\nProtein 2: RBCK1 (Q9BYM8)\n Function: E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, such as UBE2L3/UBCM4, and then transfers it to substrates (PubMed:12629548, PubMed:17449468, PubMed\n\nEvidence: Available evidence does not support a physical interaction between HGS and RBCK1.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 293, "gene_symbol_1": "HGS", "gene_symbol_2": "RBCK1", "detection_method": null}} +{"question_id": "PPIL1-0106", "task": "ppi-l1", "split": "val", "difficulty": "hard", "context_text": "Protein 1: TP53 (P04637)\n Function: Multifunctional transcription factor that induces cell cycle arrest, DNA repair or apoptosis upon binding to its target DNA sequence (PubMed:11025664, PubMed:12524540, PubMed:12810724, PubMed:15186775\n Location: Cytoplasm\n\nProtein 2: DDX5 (P17844)\n Function: Involved in the alternative regulation of pre-mRNA splicing; its RNA helicase activity is necessary for increasing tau exon 10 inclusion and occurs in a RBM4-dependent manner. Binds to the tau pre-mRN\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between TP53 and DDX5.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "gold", "result_id": 75, "gene_symbol_1": "TP53", "gene_symbol_2": "DDX5", "detection_method": null}} +{"question_id": "PPIL1-0107", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: CARD9 (Q9H257)\n Function: Adapter protein that plays a key role in innate immune response against fungi by forming signaling complexes downstream of C-type lectin receptors (PubMed:26961233, PubMed:33558980). CARD9-mediated si\n Location: Cytoplasm\n\nProtein 2: ZNF688 (A0A0S2Z6P0)\n\nEvidence: Available evidence does not support a physical interaction between CARD9 and ZNF688.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 732, "gene_symbol_1": "CARD9", "gene_symbol_2": "ZNF688", "detection_method": null}} +{"question_id": "PPIL1-0108", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: TFIP11 (Q9UBB9)\n Function: Involved in pre-mRNA splicing, specifically in spliceosome disassembly during late-stage splicing events. Intron turnover seems to proceed through reactions in two lariat-intron associated complexes t\n Location: Cytoplasm\n\nProtein 2: RNF6 (Q9Y252)\n Function: E3 ubiquitin-protein ligase mediating 'Lys-48'-linked polyubiquitination of LIMK1 and its subsequent targeting to the proteasome for degradation (By similarity). Negatively regulates axonal outgrowth \n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between TFIP11 and RNF6.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 530, "gene_symbol_1": "TFIP11", "gene_symbol_2": "RNF6", "detection_method": null}} +{"question_id": "PPIL1-0109", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: RNF6 (Q9Y252)\n Function: E3 ubiquitin-protein ligase mediating 'Lys-48'-linked polyubiquitination of LIMK1 and its subsequent targeting to the proteasome for degradation (By similarity). Negatively regulates axonal outgrowth \n Location: Nucleus\n\nProtein 2: PNMA2 (Q9UL42)\n Location: Nucleus, nucleolus\n\nEvidence: Available evidence does not support a physical interaction between RNF6 and PNMA2.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 528, "gene_symbol_1": "RNF6", "gene_symbol_2": "PNMA2", "detection_method": null}} +{"question_id": "PPIL1-0110", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: CDK11B (P21127)\n Function: Cyclin-dependent protein kinase that acts as a regulator of transcription and pre-mRNA splicing (PubMed:12501247, PubMed:18216018, PubMed:32367068, PubMed:36104565). Acts as a key regulator of pre-mRN\n Location: Nucleus\n\nProtein 2: PAK1 (Q13153)\n Function: Protein kinase involved in intracellular signaling pathways downstream of integrins and receptor-type kinases that plays an important role in cytoskeleton dynamics, in cell adhesion, migration, prolif\n Location: Cytoplasm\n\nEvidence: Available evidence does not support a physical interaction between CDK11B and PAK1.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 2, "gene_symbol_1": "CDK11B", "gene_symbol_2": "PAK1", "detection_method": null}} +{"question_id": "PPIL1-0111", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: BTRC (A0A0S2Z4Q5)\n\nProtein 2: TRIM9 (Q9C026)\n Function: E3 ubiquitin-protein ligase which ubiquitinates itself in cooperation with an E2 enzyme UBE2D2/UBC4 and serves as a targeting signal for proteasomal degradation. May play a role in regulation of neuro\n Location: Cytoplasm\n\nEvidence: Available evidence does not support a physical interaction between BTRC and TRIM9.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 198, "gene_symbol_1": "BTRC", "gene_symbol_2": "TRIM9", "detection_method": null}} +{"question_id": "PPIL1-0112", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: PIM1 (P11309)\n Function: Proto-oncogene with serine/threonine kinase activity involved in cell survival and cell proliferation and thus providing a selective advantage in tumorigenesis (PubMed:15528381, PubMed:1825810, PubMed\n Location: Cytoplasm\n\nProtein 2: BMX (P51813)\n Function: Non-receptor tyrosine kinase that plays central but diverse modulatory roles in various signaling processes involved in the regulation of actin reorganization, cell migration, cell proliferation and s\n Location: Cytoplasm\n\nEvidence: Available evidence does not support a physical interaction between PIM1 and BMX.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "gold", "result_id": 41, "gene_symbol_1": "PIM1", "gene_symbol_2": "BMX", "detection_method": null}} +{"question_id": "PPIL1-0113", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: RXRB (P28702)\n Function: Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression \n Location: Nucleus\n\nProtein 2: TRIM37 (O94972)\n Function: E3 ubiquitin-protein ligase required to prevent centriole reduplication (PubMed:15885686, PubMed:23769972). Probably acts by ubiquitinating positive regulators of centriole reduplication (PubMed:23769\n Location: Chromosome\n\nEvidence: Available evidence does not support a physical interaction between RXRB and TRIM37.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 542, "gene_symbol_1": "RXRB", "gene_symbol_2": "TRIM37", "detection_method": null}} +{"question_id": "PPIL1-0114", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: AP3B1 (A0A0S2Z4X7)\n\nProtein 2: GKAP1 (Q5VSY0)\n Function: Regulates insulin-dependent IRS1 tyrosine phosphorylation in adipocytes by modulating the availability of IRS1 to IR tyrosine kinase. Its association with IRS1 is required for insulin-induced transloc\n Location: Golgi apparatus\n\nEvidence: Available evidence does not support a physical interaction between AP3B1 and GKAP1.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 136, "gene_symbol_1": "AP3B1", "gene_symbol_2": "GKAP1", "detection_method": null}} +{"question_id": "PPIL1-0115", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: ACBD4 (Q8NC06)\n Function: Binds medium- and long-chain acyl-CoA esters and may function as an intracellular carrier of acyl-CoA esters\n\nProtein 2: MEOX2 (Q6FHY5)\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between ACBD4 and MEOX2.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 117, "gene_symbol_1": "ACBD4", "gene_symbol_2": "MEOX2", "detection_method": null}} +{"question_id": "PPIL1-0116", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: ACBD4 (A0A0S2Z5W7)\n\nProtein 2: KIF9 (Q9HAQ2)\n Function: Essential for normal male fertility and for progressive motility of spermatozoa\n Location: Cytoplasm, cytoskeleton\n\nEvidence: Available evidence does not support a physical interaction between ACBD4 and KIF9.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 121, "gene_symbol_1": "ACBD4", "gene_symbol_2": "KIF9", "detection_method": null}} +{"question_id": "PPIL1-0117", "task": "ppi-l1", "split": "val", "difficulty": "hard", "context_text": "Protein 1: ACBD4 (Q8NC06)\n Function: Binds medium- and long-chain acyl-CoA esters and may function as an intracellular carrier of acyl-CoA esters\n\nProtein 2: KIF9 (Q9HAQ2)\n Function: Essential for normal male fertility and for progressive motility of spermatozoa\n Location: Cytoplasm, cytoskeleton\n\nEvidence: Available evidence does not support a physical interaction between ACBD4 and KIF9.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 116, "gene_symbol_1": "ACBD4", "gene_symbol_2": "KIF9", "detection_method": null}} +{"question_id": "PPIL1-0118", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: BANP (E9PJI6)\n\nProtein 2: BLZF1 (Q9H2G9)\n Function: Required for normal Golgi structure and for protein transport from the endoplasmic reticulum (ER) through the Golgi apparatus to the cell surface\n Location: Golgi apparatus membrane\n\nEvidence: Available evidence does not support a physical interaction between BANP and BLZF1.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 164, "gene_symbol_1": "BANP", "gene_symbol_2": "BLZF1", "detection_method": null}} +{"question_id": "PPIL1-0119", "task": "ppi-l1", "split": "val", "difficulty": "hard", "context_text": "Protein 1: FANCG (A0A0S2Z3W0)\n\nProtein 2: TADA2A (O75478)\n Function: Component of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4. Required for the function of some acidic activation domains, which activate transcription from a\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between FANCG and TADA2A.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 276, "gene_symbol_1": "FANCG", "gene_symbol_2": "TADA2A", "detection_method": null}} +{"question_id": "PPIL1-0120", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: EEF1AKMT1 (Q8WVE0)\n Function: Protein N-lysine methyltransferase that selectively catalyzes the trimethylation of EEF1A at 'Lys-79'\n Location: Cytoplasm\n\nProtein 2: NDN (Q99608)\n Function: Growth suppressor that facilitates the entry of the cell into cell cycle arrest. Functionally similar to the retinoblastoma protein it binds to and represses the activity of cell-cycle-promoting prote\n Location: Perikaryon\n\nEvidence: Available evidence does not support a physical interaction between EEF1AKMT1 and NDN.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 440, "gene_symbol_1": "EEF1AKMT1", "gene_symbol_2": "NDN", "detection_method": null}} +{"question_id": "PPIL1-0121", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: SPAST (Q9UBP0)\n Function: ATP-dependent microtubule severing protein that specifically recognizes and cuts microtubules that are polyglutamylated (PubMed:11809724, PubMed:15716377, PubMed:16219033, PubMed:17389232, PubMed:2053\n Location: Membrane\n\nProtein 2: FBXL17 (Q9UF56)\n Function: Substrate-recognition component of the SCF(FBXL17) E3 ubiquitin ligase complex, a key component of a quality control pathway required to ensure functional dimerization of BTB domain-containing protein\n Location: Cytoplasm\n\nEvidence: Available evidence does not support a physical interaction between SPAST and FBXL17.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 772, "gene_symbol_1": "SPAST", "gene_symbol_2": "FBXL17", "detection_method": null}} +{"question_id": "PPIL1-0122", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: RINT1 (Q6NUQ1)\n Function: Involved in regulation of membrane traffic between the Golgi and the endoplasmic reticulum (ER); the function is proposed to depend on its association in the NRZ complex which is believed to play a ro\n Location: Cytoplasm\n\nProtein 2: RNF6 (Q9Y252)\n Function: E3 ubiquitin-protein ligase mediating 'Lys-48'-linked polyubiquitination of LIMK1 and its subsequent targeting to the proteasome for degradation (By similarity). Negatively regulates axonal outgrowth \n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between RINT1 and RNF6.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 516, "gene_symbol_1": "RINT1", "gene_symbol_2": "RNF6", "detection_method": null}} +{"question_id": "PPIL1-0123", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: GJA1 (P17302)\n Function: Structural component of the gap junction, a specialized intercellular structure consisting of a cluster of closely packed pairs of transmembrane channels, the connexons, that allow passage of small mo\n Location: Cell membrane\n\nProtein 2: DSC2 (Q02487)\n Function: A component of desmosome cell-cell junctions which are required for positive regulation of cellular adhesion (PubMed:33596089). Promotes timely incorporation of DSG2 into desmosome intercellular junct\n Location: Cell membrane\n\nEvidence: Available evidence does not support a physical interaction between GJA1 and DSC2.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "gold", "result_id": 88, "gene_symbol_1": "GJA1", "gene_symbol_2": "DSC2", "detection_method": null}} +{"question_id": "PPIL1-0124", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: TADA2A (O75478)\n Function: Component of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4. Required for the function of some acidic activation domains, which activate transcription from a\n Location: Nucleus\n\nProtein 2: RNF6 (Q9Y252)\n Function: E3 ubiquitin-protein ligase mediating 'Lys-48'-linked polyubiquitination of LIMK1 and its subsequent targeting to the proteasome for degradation (By similarity). Negatively regulates axonal outgrowth \n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between TADA2A and RNF6.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 531, "gene_symbol_1": "TADA2A", "gene_symbol_2": "RNF6", "detection_method": null}} +{"question_id": "PPIL1-0125", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: SDC3 (A0A9K3Y886)\n Function: Cell surface proteoglycan\n Location: Membrane\n\nProtein 2: KRTAP4-2 (Q9BYR5)\n Function: In the hair cortex, hair keratin intermediate filaments are embedded in an interfilamentous matrix, consisting of hair keratin-associated proteins (KRTAP), which are essential for the formation of a r\n\nEvidence: Available evidence does not support a physical interaction between SDC3 and KRTAP4-2.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 583, "gene_symbol_1": "SDC3", "gene_symbol_2": "KRTAP4-2", "detection_method": null}} +{"question_id": "PPIL1-0126", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: CALCOCO2 (Q13137)\n Function: Xenophagy-specific receptor required for autophagy-mediated intracellular bacteria degradation. Acts as an effector protein of galectin-sensed membrane damage that restricts the proliferation of infec\n Location: Cytoplasm, perinuclear region\n\nProtein 2: RXRB (P28702)\n Function: Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression \n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between CALCOCO2 and RXRB.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 557, "gene_symbol_1": "CALCOCO2", "gene_symbol_2": "RXRB", "detection_method": null}} +{"question_id": "PPIL1-0127", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: RXRB (P28702)\n Function: Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression \n Location: Nucleus\n\nProtein 2: CCDC57 (Q2TAC2)\n Function: Pleiotropic regulator of centriole duplication, mitosis, and ciliogenesis. Critical interface between centrosome and microtubule-mediated cellular processes. Centriole duplication protein required for\n Location: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome\n\nEvidence: Available evidence does not support a physical interaction between RXRB and CCDC57.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 560, "gene_symbol_1": "RXRB", "gene_symbol_2": "CCDC57", "detection_method": null}} +{"question_id": "PPIL1-0128", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: UBQLN1 (Q9UMX0)\n Function: Plays an important role in the regulation of different protein degradation mechanisms and pathways including ubiquitin-proteasome system (UPS), autophagy and endoplasmic reticulum-associated protein d\n Location: Cytoplasm\n\nProtein 2: COPS4 (Q9BT78)\n Function: Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathwa\n Location: Cytoplasm\n\nEvidence: Available evidence does not support a physical interaction between UBQLN1 and COPS4.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 223, "gene_symbol_1": "UBQLN1", "gene_symbol_2": "COPS4", "detection_method": null}} +{"question_id": "PPIL1-0129", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: FOXP2 (O15409)\n Function: Transcriptional repressor that may play a role in the specification and differentiation of lung epithelium. May also play a role in developing neural, gastrointestinal and cardiovascular tissues. Can \n Location: Nucleus\n\nProtein 2: TBR1 (Q16650)\n Function: Transcriptional repressor involved in multiple aspects of cortical development, including neuronal migration, laminar and areal identity, and axonal projection (PubMed:25232744, PubMed:30250039). As t\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between FOXP2 and TBR1.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 758, "gene_symbol_1": "FOXP2", "gene_symbol_2": "TBR1", "detection_method": null}} +{"question_id": "PPIL1-0130", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: S100A1 (P23297)\n Function: Small calcium binding protein that plays important roles in several biological processes such as Ca(2+) homeostasis, chondrocyte biology and cardiomyocyte regulation (PubMed:12804600). In response to \n Location: Cytoplasm\n\nProtein 2: S100B (A8MRB1)\n\nEvidence: Available evidence does not support a physical interaction between S100A1 and S100B.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 576, "gene_symbol_1": "S100A1", "gene_symbol_2": "S100B", "detection_method": null}} +{"question_id": "PPIL1-0131", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: TRIM2 (Q9C040)\n Function: UBE2D1-dependent E3 ubiquitin-protein ligase that mediates the ubiquitination of NEFL and of phosphorylated BCL2L11. Plays a neuroprotective function. May play a role in neuronal rapid ischemic tolera\n Location: Cytoplasm\n\nProtein 2: CDK20 (A0A0S2Z535)\n Function: Required for high-level Shh responses in the developing neural tube. Together with TBC1D32, controls the structure of the primary cilium by coordinating assembly of the ciliary membrane and axoneme, a\n Location: Cell projection, cilium\n\nEvidence: Available evidence does not support a physical interaction between TRIM2 and CDK20.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 234, "gene_symbol_1": "TRIM2", "gene_symbol_2": "CDK20", "detection_method": null}} +{"question_id": "PPIL1-0132", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: ABCF3 (Q9NUQ8)\n Function: Displays an antiviral effect against flaviviruses such as west Nile virus (WNV) in the presence of OAS1B\n\nProtein 2: MDFI (Q99750)\n Function: Inhibits the transactivation activity of the Myod family of myogenic factors and represses myogenesis (By similarity). Acts by associating with Myod family members and retaining them in the cytoplasm \n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between ABCF3 and MDFI.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 125, "gene_symbol_1": "ABCF3", "gene_symbol_2": "MDFI", "detection_method": null}} +{"question_id": "PPIL1-0133", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: APBA1 (Q02410)\n Function: Putative function in synaptic vesicle exocytosis by binding to Munc18-1, an essential component of the synaptic vesicle exocytotic machinery. May modulate processing of the amyloid-beta precursor prot\n Location: Cytoplasm\n\nProtein 2: RAB6B (Q9NRW1)\n Function: The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between active GTP-bound and inactiv\n Location: Golgi apparatus membrane\n\nEvidence: Available evidence does not support a physical interaction between APBA1 and RAB6B.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 100, "gene_symbol_1": "APBA1", "gene_symbol_2": "RAB6B", "detection_method": null}} +{"question_id": "PPIL1-0134", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: UBQLN1 (Q9UMX0)\n Function: Plays an important role in the regulation of different protein degradation mechanisms and pathways including ubiquitin-proteasome system (UPS), autophagy and endoplasmic reticulum-associated protein d\n Location: Cytoplasm\n\nProtein 2: CD99L2 (A0A0S2Z5R8)\n Function: Plays a role in a late step of leukocyte extravasation helping cells to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Homophilic adhesion molecule\n Location: Cell junction\n\nEvidence: Available evidence does not support a physical interaction between UBQLN1 and CD99L2.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 226, "gene_symbol_1": "UBQLN1", "gene_symbol_2": "CD99L2", "detection_method": null}} +{"question_id": "PPIL1-0135", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: ESR1 (P03372)\n Function: Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues.\n Location: Nucleus\n\nProtein 2: TXNRD1 (Q16881)\n Function: Reduces disulfide protein thioredoxin (Trx) to its dithiol-containing form (PubMed:8577704). Homodimeric flavoprotein involved in the regulation of cellular redox reactions, growth and differentiation\n Location: Cytoplasm\n\nEvidence: Available evidence does not support a physical interaction between ESR1 and TXNRD1.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "gold", "result_id": 107, "gene_symbol_1": "ESR1", "gene_symbol_2": "TXNRD1", "detection_method": null}} +{"question_id": "PPIL1-0136", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: NDN (A0A0S2Z3Y6)\n\nProtein 2: EEF1AKMT1 (Q8WVE0)\n Function: Protein N-lysine methyltransferase that selectively catalyzes the trimethylation of EEF1A at 'Lys-79'\n Location: Cytoplasm\n\nEvidence: Available evidence does not support a physical interaction between NDN and EEF1AKMT1.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 434, "gene_symbol_1": "NDN", "gene_symbol_2": "EEF1AKMT1", "detection_method": null}} +{"question_id": "PPIL1-0137", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: HNRPLL (A0A0S2Z5U3)\n\nProtein 2: DDX39A (O00148)\n Function: Helicase that plays an essential role in mRNA export and is involved in multiple steps in RNA metabolism including alternative splicing (PubMed:33941617, PubMed:38801080). Regulates nuclear mRNA expor\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between HNRPLL and DDX39A.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 341, "gene_symbol_1": "HNRPLL", "gene_symbol_2": "DDX39A", "detection_method": null}} +{"question_id": "PPIL1-0138", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: ACBD4 (A0A0S2Z5W7)\n\nProtein 2: MEOX1 (P50221)\n Function: Mesodermal transcription factor that plays a key role in somitogenesis and is specifically required for sclerotome development. Required for maintenance of the sclerotome polarity and formation of the\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between ACBD4 and MEOX1.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 130, "gene_symbol_1": "ACBD4", "gene_symbol_2": "MEOX1", "detection_method": null}} +{"question_id": "PPIL1-0139", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: NDN (A0A0S2Z3Z2)\n\nProtein 2: LAX1 (Q8IWV1)\n Function: Negatively regulates TCR (T-cell antigen receptor)-mediated signaling in T-cells and BCR (B-cell antigen receptor)-mediated signaling in B-cells\n Location: Cell membrane\n\nEvidence: Available evidence does not support a physical interaction between NDN and LAX1.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 430, "gene_symbol_1": "NDN", "gene_symbol_2": "LAX1", "detection_method": null}} +{"question_id": "PPIL1-0140", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: CCND2 (P30279)\n Function: Regulatory component of the cyclin D2-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S tran\n Location: Nucleus\n\nProtein 2: CDK5 (Q00535)\n Function: Proline-directed serine/threonine-protein kinase essential for neuronal cell cycle arrest and differentiation and may be involved in apoptotic cell death in neuronal diseases by triggering abortive ce\n Location: Cytoplasm\n\nEvidence: Available evidence does not support a physical interaction between CCND2 and CDK5.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 227, "gene_symbol_1": "CCND2", "gene_symbol_2": "CDK5", "detection_method": null}} +{"question_id": "PPIL1-0141", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: TNS2 (Q63HR2)\n Function: Tyrosine-protein phosphatase which regulates cell motility, proliferation and muscle-response to insulin (PubMed:15817639, PubMed:23401856). Phosphatase activity is mediated by binding to phosphatidyl\n Location: Cell junction, focal adhesion\n\nProtein 2: RNF6 (Q9Y252)\n Function: E3 ubiquitin-protein ligase mediating 'Lys-48'-linked polyubiquitination of LIMK1 and its subsequent targeting to the proteasome for degradation (By similarity). Negatively regulates axonal outgrowth \n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between TNS2 and RNF6.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 511, "gene_symbol_1": "TNS2", "gene_symbol_2": "RNF6", "detection_method": null}} +{"question_id": "PPIL1-0142", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: GMPPA (Q96IJ6)\n Function: Regulatory subunit of the GMPPA-GMPPB mannose-1-phosphate guanylyltransferase complex; reduces the catalytic activity of GMPPB when part of the complex (PubMed:24035193, PubMed:33986552). Mediates all\n Location: Cytoplasm\n\nProtein 2: ZNF688 (A0A0S2Z5X4)\n\nEvidence: Available evidence does not support a physical interaction between GMPPA and ZNF688.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 697, "gene_symbol_1": "GMPPA", "gene_symbol_2": "ZNF688", "detection_method": null}} +{"question_id": "PPIL1-0143", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: TERT (O14746)\n Function: Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. Active in progenitor and cancer cells. Inactive, or very low activity, in normal somati\n Location: Nucleus, nucleolus\n\nProtein 2: PML (P29590)\n Function: Functions via its association with PML-nuclear bodies (PML-NBs) in a wide range of important cellular processes, including tumor suppression, transcriptional regulation, apoptosis, senescence, DNA dam\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between TERT and PML.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 99, "gene_symbol_1": "TERT", "gene_symbol_2": "PML", "detection_method": null}} +{"question_id": "PPIL1-0144", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: GATA3 (P23771)\n Function: Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. Required for the T-helper 2 (Th2) differentiation proc\n Location: Nucleus\n\nProtein 2: LCK (P06239)\n Function: Non-receptor tyrosine-protein kinase that plays an essential role in the selection and maturation of developing T-cells in the thymus and in the function of mature T-cells (PubMed:2470098). Plays a ke\n Location: Cell membrane\n\nEvidence: Available evidence does not support a physical interaction between GATA3 and LCK.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 376, "gene_symbol_1": "GATA3", "gene_symbol_2": "LCK", "detection_method": null}} +{"question_id": "PPIL1-0145", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: HNRNPD (Q14103)\n Function: Binds with high affinity to RNA molecules that contain AU-rich elements (AREs) found within the 3'-UTR of many proto-oncogenes and cytokine mRNAs. Also binds to double- and single-stranded DNA sequenc\n Location: Nucleus\n\nProtein 2: YWHAG (P61981)\n Function: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways (PubMed:15696159, PubMed:16511572, PubMed:36732624). Binds to a large number of part\n Location: Cytoplasm, cytosol\n\nEvidence: Available evidence does not support a physical interaction between HNRNPD and YWHAG.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "gold", "result_id": 55, "gene_symbol_1": "HNRNPD", "gene_symbol_2": "YWHAG", "detection_method": null}} +{"question_id": "PPIL1-0146", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: ITGB2 (P05107)\n Function: Integrin ITGAL:ITGB2 is a receptor for ICAM1, ICAM2 and ICAM3 (PubMed:1676048, PubMed:23775590, PubMed:38195629). Integrin ITGAL:ITGB2 is also a receptor for the secreted form of ubiquitin-like protei\n Location: Cell membrane\n\nProtein 2: ANKS1B (Q7Z6G8)\n Function: Isoform 2 may participate in the regulation of nucleoplasmic coilin protein interactions in neuronal and transformed cells\n Location: Cytoplasm\n\nEvidence: Available evidence does not support a physical interaction between ITGB2 and ANKS1B.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "gold", "result_id": 12, "gene_symbol_1": "ITGB2", "gene_symbol_2": "ANKS1B", "detection_method": null}} +{"question_id": "PPIL1-0147", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: MEOX2 (Q6FHY5)\n Location: Nucleus\n\nProtein 2: HYCC1 (Q9BYI3)\n Function: Component of a complex required to localize phosphatidylinositol 4-kinase (PI4K) to the plasma membrane (PubMed:26571211). The complex acts as a regulator of phosphatidylinositol 4-phosphate (PtdIns(4\n Location: Cytoplasm, cytosol\n\nEvidence: Available evidence does not support a physical interaction between MEOX2 and HYCC1.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 261, "gene_symbol_1": "MEOX2", "gene_symbol_2": "HYCC1", "detection_method": null}} +{"question_id": "PPIL1-0148", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: AGTRAP (Q6RW13)\n Function: Appears to be a negative regulator of type-1 angiotensin II receptor-mediated signaling by regulating receptor internalization as well as mechanism of receptor desensitization such as phosphorylation.\n Location: Endoplasmic reticulum membrane\n\nProtein 2: S100B (P04271)\n Function: Small zinc- and- and calcium-binding protein that is highly expressed in astrocytes and constitutes one of the most abundant soluble proteins in brain (PubMed:20950652, PubMed:6487634). Weakly binds c\n Location: Cytoplasm\n\nEvidence: Available evidence does not support a physical interaction between AGTRAP and S100B.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 599, "gene_symbol_1": "AGTRAP", "gene_symbol_2": "S100B", "detection_method": null}} +{"question_id": "PPIL1-0149", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: PIMREG (Q9BSJ6)\n Function: During mitosis, may play a role in the control of metaphase-to-anaphase transition\n Location: Nucleus\n\nProtein 2: RINT1 (Q6NUQ1)\n Function: Involved in regulation of membrane traffic between the Golgi and the endoplasmic reticulum (ER); the function is proposed to depend on its association in the NRZ complex which is believed to play a ro\n Location: Cytoplasm\n\nEvidence: Available evidence does not support a physical interaction between PIMREG and RINT1.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 262, "gene_symbol_1": "PIMREG", "gene_symbol_2": "RINT1", "detection_method": null}} +{"question_id": "PPIL1-0150", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: NLRP5 (P59047)\n Function: Component of the subcortical maternal complex (SCMC), a multiprotein complex that plays a key role in early embryonic development. The SCMC complex is a structural constituent of cytoplasmic lattices,\n Location: Cytoplasm\n\nProtein 2: TLE6 (Q9H808)\n Function: Component of the subcortical maternal complex (SCMC), a multiprotein complex that plays a key role in early embryonic development (PubMed:26537248). The SCMC complex is a structural constituent of cyt\n Location: Cytoplasm\n\nEvidence: Available evidence does not support a physical interaction between NLRP5 and TLE6.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 767, "gene_symbol_1": "NLRP5", "gene_symbol_2": "TLE6", "detection_method": null}} +{"question_id": "PPIL1-0151", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: BAG1 (A0A0S2Z3F5)\n\nProtein 2: HSPA8 (P11142)\n Function: Molecular chaperone implicated in a wide variety of cellular processes, including protection of the proteome from stress, folding and transport of newly synthesized polypeptides, chaperone-mediated au\n Location: Cytoplasm\n\nEvidence: Available evidence does not support a physical interaction between BAG1 and HSPA8.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 155, "gene_symbol_1": "BAG1", "gene_symbol_2": "HSPA8", "detection_method": null}} +{"question_id": "PPIL1-0152", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: S100B (P04271)\n Function: Small zinc- and- and calcium-binding protein that is highly expressed in astrocytes and constitutes one of the most abundant soluble proteins in brain (PubMed:20950652, PubMed:6487634). Weakly binds c\n Location: Cytoplasm\n\nProtein 2: RABAC1 (Q9UI14)\n Function: General Rab protein regulator required for vesicle formation from the Golgi complex. May control vesicle docking and fusion by mediating the action of Rab GTPases to the SNARE complexes. In addition i\n Location: Cell membrane\n\nEvidence: Available evidence does not support a physical interaction between S100B and RABAC1.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 580, "gene_symbol_1": "S100B", "gene_symbol_2": "RABAC1", "detection_method": null}} +{"question_id": "PPIL1-0153", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: TP53 (P04637)\n Function: Multifunctional transcription factor that induces cell cycle arrest, DNA repair or apoptosis upon binding to its target DNA sequence (PubMed:11025664, PubMed:12524540, PubMed:12810724, PubMed:15186775\n Location: Cytoplasm\n\nProtein 2: DDX5 (P17844)\n Function: Involved in the alternative regulation of pre-mRNA splicing; its RNA helicase activity is necessary for increasing tau exon 10 inclusion and occurs in a RBM4-dependent manner. Binds to the tau pre-mRN\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between TP53 and DDX5.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "gold", "result_id": 73, "gene_symbol_1": "TP53", "gene_symbol_2": "DDX5", "detection_method": null}} +{"question_id": "PPIL1-0154", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: UBQLN1 (Q9UMX0)\n Function: Plays an important role in the regulation of different protein degradation mechanisms and pathways including ubiquitin-proteasome system (UPS), autophagy and endoplasmic reticulum-associated protein d\n Location: Cytoplasm\n\nProtein 2: CD99L2 (A0A0S2Z5R8)\n Function: Plays a role in a late step of leukocyte extravasation helping cells to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Homophilic adhesion molecule\n Location: Cell junction\n\nEvidence: Available evidence does not support a physical interaction between UBQLN1 and CD99L2.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 235, "gene_symbol_1": "UBQLN1", "gene_symbol_2": "CD99L2", "detection_method": null}} +{"question_id": "PPIL1-0155", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: FASLG (P48023)\n Function: Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells (PubMed:26334989, PubMed:9228058). Involved in cytotoxic T-cell-mediated apoptosis, natural killer cell-m\n Location: Cell membrane\n\nProtein 2: NCK1 (A0A0S2Z4E4)\n\nEvidence: Available evidence does not support a physical interaction between FASLG and NCK1.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 415, "gene_symbol_1": "FASLG", "gene_symbol_2": "NCK1", "detection_method": null}} +{"question_id": "PPIL1-0156", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: TADA2A (O75478)\n Function: Component of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4. Required for the function of some acidic activation domains, which activate transcription from a\n Location: Nucleus\n\nProtein 2: FANCG (A0A0S2Z3S1)\n Function: DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. May be implicated in interstrand DNA cross-link repair and in the maintenance of normal chromosome \n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between TADA2A and FANCG.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 280, "gene_symbol_1": "TADA2A", "gene_symbol_2": "FANCG", "detection_method": null}} +{"question_id": "PPIL1-0157", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: HRAS (P01112)\n Function: Involved in the activation of Ras protein signal transduction (PubMed:22821884). Ras proteins bind GDP/GTP and possess intrinsic GTPase activity (PubMed:12740440, PubMed:14500341, PubMed:9020151)\n Location: Cell membrane\n\nProtein 2: RIN1 (Q13671)\n Function: Ras effector protein, which may serve as an inhibitory modulator of neuronal plasticity in aversive memory formation. Can affect Ras signaling at different levels. First, by competing with RAF1 protei\n Location: Cytoplasm\n\nEvidence: Available evidence does not support a physical interaction between HRAS and RIN1.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "gold", "result_id": 65, "gene_symbol_1": "HRAS", "gene_symbol_2": "RIN1", "detection_method": null}} +{"question_id": "PPIL1-0158", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: PRMT1 (Q99873)\n Function: Arginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, FMR1, ILF3, HNRNPA1,\n Location: Nucleus\n\nProtein 2: CTDP1 (Q9Y5B0)\n Function: Processively dephosphorylates 'Ser-2' and 'Ser-5' of the heptad repeats YSPTSPS in the C-terminal domain of the largest RNA polymerase II subunit. This promotes the activity of RNA polymerase II. Play\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between PRMT1 and CTDP1.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "gold", "result_id": 32, "gene_symbol_1": "PRMT1", "gene_symbol_2": "CTDP1", "detection_method": null}} +{"question_id": "PPIL1-0159", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: PIMREG (Q9BSJ6)\n Function: During mitosis, may play a role in the control of metaphase-to-anaphase transition\n Location: Nucleus\n\nProtein 2: SIAH1 (Q8IUQ4)\n Function: E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins (PubMed:14506261, PubMed:14645235, PubMed:14654780, PubMed:15064394, PubMed:16085652,\n Location: Cytoplasm\n\nEvidence: Available evidence does not support a physical interaction between PIMREG and SIAH1.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 279, "gene_symbol_1": "PIMREG", "gene_symbol_2": "SIAH1", "detection_method": null}} +{"question_id": "PPIL1-0160", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: DIABLO (Q9NR28)\n Function: Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC\n Location: Mitochondrion\n\nProtein 2: AGTRAP (Q6RW13)\n Function: Appears to be a negative regulator of type-1 angiotensin II receptor-mediated signaling by regulating receptor internalization as well as mechanism of receptor desensitization such as phosphorylation.\n Location: Endoplasmic reticulum membrane\n\nEvidence: Available evidence does not support a physical interaction between DIABLO and AGTRAP.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 244, "gene_symbol_1": "DIABLO", "gene_symbol_2": "AGTRAP", "detection_method": null}} +{"question_id": "PPIL1-0161", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: MIPOL1 (A0A0S2Z674)\n\nProtein 2: TTC12 (Q9H892)\n Function: Cytoplasmic protein that plays a role in the proper assembly of dynein arm complexes in motile cilia in both respiratory cells and sperm flagella\n Location: Cytoplasm\n\nEvidence: Available evidence does not support a physical interaction between MIPOL1 and TTC12.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 392, "gene_symbol_1": "MIPOL1", "gene_symbol_2": "TTC12", "detection_method": null}} +{"question_id": "PPIL1-0162", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: HTRA2 (O43464)\n Function: Serine protease that shows proteolytic activity against a non-specific substrate beta-casein (PubMed:10873535). Promotes apoptosis by either relieving the inhibition of BIRC proteins on caspases, lead\n Location: Mitochondrion intermembrane space\n\nProtein 2: RIPK1 (Q13546)\n Function: Serine-threonine kinase which is a key regulator of TNF-mediated apoptosis, necroptosis and inflammatory pathways (PubMed:17703191, PubMed:24144979, PubMed:31827280, PubMed:31827281, PubMed:32657447, \n Location: Cytoplasm\n\nEvidence: Available evidence does not support a physical interaction between HTRA2 and RIPK1.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 78, "gene_symbol_1": "HTRA2", "gene_symbol_2": "RIPK1", "detection_method": null}} +{"question_id": "PPIL1-0163", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: FHL3 (Q13643)\n Function: Recruited by SOX15 to FOXK1 promoters where it acts as a transcriptional coactivator of FOXK1\n Location: Nucleus\n\nProtein 2: FAM126A (A0A0S2Z6M9)\n Location: Cell membrane\n\nEvidence: Available evidence does not support a physical interaction between FHL3 and FAM126A.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 264, "gene_symbol_1": "FHL3", "gene_symbol_2": "FAM126A", "detection_method": null}} +{"question_id": "PPIL1-0164", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: S100B (P04271)\n Function: Small zinc- and- and calcium-binding protein that is highly expressed in astrocytes and constitutes one of the most abundant soluble proteins in brain (PubMed:20950652, PubMed:6487634). Weakly binds c\n Location: Cytoplasm\n\nProtein 2: S100B (A8MRB1)\n\nEvidence: Available evidence does not support a physical interaction between S100B and S100B.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 579, "gene_symbol_1": "S100B", "gene_symbol_2": "S100B", "detection_method": null}} +{"question_id": "PPIL1-0165", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: FOXM1 (Q08050)\n Function: Transcription factor regulating the expression of cell cycle genes essential for DNA replication and mitosis (PubMed:19160488, PubMed:20360045). Plays a role in the control of cell proliferation (PubM\n Location: Nucleus\n\nProtein 2: BANP (B4DE54)\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between FOXM1 and BANP.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 179, "gene_symbol_1": "FOXM1", "gene_symbol_2": "BANP", "detection_method": null}} +{"question_id": "PPIL1-0166", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: NDRG4 (Q9ULP0)\n Function: Contributes to the maintenance of intracerebral BDNF levels within the normal range, which is necessary for the preservation of spatial learning and the resistance to neuronal cell death caused by isc\n Location: Cytoplasm, cytosol\n\nProtein 2: RTN3 (O95197)\n Function: May be involved in membrane trafficking in the early secretory pathway. Inhibits BACE1 activity and amyloid precursor protein processing. May induce caspase-8 cascade and apoptosis. May favor BCL2 tra\n Location: Endoplasmic reticulum membrane\n\nEvidence: Available evidence does not support a physical interaction between NDRG4 and RTN3.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 472, "gene_symbol_1": "NDRG4", "gene_symbol_2": "RTN3", "detection_method": null}} +{"question_id": "PPIL1-0167", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: BANP (E9PJI6)\n\nProtein 2: CEP76 (Q8TAP6)\n Function: Centrosomal protein involved in regulation of centriole duplication. Required to limit centriole duplication to once per cell cycle by preventing centriole reduplication\n Location: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome\n\nEvidence: Available evidence does not support a physical interaction between BANP and CEP76.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 183, "gene_symbol_1": "BANP", "gene_symbol_2": "CEP76", "detection_method": null}} +{"question_id": "PPIL1-0168", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: SLC30A2 (Q9BRI3)\n Function: Electroneutral proton-coupled antiporter concentrating zinc ions into a variety of intracellular organelles including endosomes, zymogen granules and mitochondria. Thereby, plays a crucial role in cel\n Location: Cytoplasmic vesicle, secretory vesicle membrane\n\nProtein 2: SERPING1 (P05155)\n Function: Serine protease inhibitor, which acrs as a regulator of the classical complement pathway (PubMed:10946292, PubMed:11527969, PubMed:3458172, PubMed:6416294). Forms a proteolytically inactive stoichiome\n Location: Secreted\n\nEvidence: Available evidence does not support a physical interaction between SLC30A2 and SERPING1.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 603, "gene_symbol_1": "SLC30A2", "gene_symbol_2": "SERPING1", "detection_method": null}} +{"question_id": "PPIL1-0169", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: CCNDBP1 (O95273)\n Function: May negatively regulate cell cycle progression. May act at least in part via inhibition of the cyclin-D1/CDK4 complex, thereby preventing phosphorylation of RB1 and blocking E2F-dependent transcriptio\n Location: Cytoplasm\n\nProtein 2: FH (P07954)\n Function: Catalyzes the reversible stereospecific interconversion of fumarate to L-malate (PubMed:30761759). Experiments in other species have demonstrated that specific isoforms of this protein act in defined \n Location: Mitochondrion\n\nEvidence: Available evidence does not support a physical interaction between CCNDBP1 and FH.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 296, "gene_symbol_1": "CCNDBP1", "gene_symbol_2": "FH", "detection_method": null}} +{"question_id": "PPIL1-0170", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: YWHAZ (P63104)\n Function: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways (PubMed:14578935, PubMed:15071501, PubMed:15644438, PubMed:16376338, PubMed:16959763\n Location: Cytoplasm\n\nProtein 2: HNRNPD (Q14103)\n Function: Binds with high affinity to RNA molecules that contain AU-rich elements (AREs) found within the 3'-UTR of many proto-oncogenes and cytokine mRNAs. Also binds to double- and single-stranded DNA sequenc\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between YWHAZ and HNRNPD.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "gold", "result_id": 58, "gene_symbol_1": "YWHAZ", "gene_symbol_2": "HNRNPD", "detection_method": null}} +{"question_id": "PPIL1-0171", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: REL (Q04864)\n Function: Proto-oncogene that may play a role in differentiation and lymphopoiesis. NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological \n Location: Nucleus\n\nProtein 2: C6orf142 (A0A0S2Z5Z3)\n\nEvidence: Available evidence does not support a physical interaction between REL and C6orf142.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 205, "gene_symbol_1": "REL", "gene_symbol_2": "C6orf142", "detection_method": null}} +{"question_id": "PPIL1-0172", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: TADA2A (O75478)\n Function: Component of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4. Required for the function of some acidic activation domains, which activate transcription from a\n Location: Nucleus\n\nProtein 2: HGS (A0A0S2Z4Q4)\n Location: Cytoplasm\n\nEvidence: Available evidence does not support a physical interaction between TADA2A and HGS.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 318, "gene_symbol_1": "TADA2A", "gene_symbol_2": "HGS", "detection_method": null}} +{"question_id": "PPIL1-0173", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: SIAH1 (Q8IUQ4)\n Function: E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins (PubMed:14506261, PubMed:14645235, PubMed:14654780, PubMed:15064394, PubMed:16085652,\n Location: Cytoplasm\n\nProtein 2: UPP2 (A0A0S2Z698)\n\nEvidence: Available evidence does not support a physical interaction between SIAH1 and UPP2.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 624, "gene_symbol_1": "SIAH1", "gene_symbol_2": "UPP2", "detection_method": null}} +{"question_id": "PPIL1-0174", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: BCL2L1 (Q07817)\n Function: Potent inhibitor of cell death. Inhibits activation of caspases. Appears to regulate cell death by blocking the voltage-dependent anion channel (VDAC) by binding to it and preventing the release of th\n Location: Mitochondrion inner membrane\n\nProtein 2: VAC14 (Q08AM6)\n Function: Scaffold protein component of the PI(3,5)P2 regulatory complex which regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Pentamerizes into a star-shaped\n Location: Endosome membrane\n\nEvidence: Available evidence does not support a physical interaction between BCL2L1 and VAC14.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 206, "gene_symbol_1": "BCL2L1", "gene_symbol_2": "VAC14", "detection_method": null}} +{"question_id": "PPIL1-0175", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: HNRPLL (A0A0S2Z5U3)\n\nProtein 2: SKIL (P12757)\n Function: May have regulatory role in cell division or differentiation in response to extracellular signals\n\nEvidence: Available evidence does not support a physical interaction between HNRPLL and SKIL.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 303, "gene_symbol_1": "HNRPLL", "gene_symbol_2": "SKIL", "detection_method": null}} +{"question_id": "PPIL1-0176", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: NCK1 (P16333)\n Function: Adapter protein which associates with tyrosine-phosphorylated growth factor receptors, such as KDR and PDGFRB, or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting\n Location: Cytoplasm\n\nProtein 2: GMPPA (Q96IJ6)\n Function: Regulatory subunit of the GMPPA-GMPPB mannose-1-phosphate guanylyltransferase complex; reduces the catalytic activity of GMPPB when part of the complex (PubMed:24035193, PubMed:33986552). Mediates all\n Location: Cytoplasm\n\nEvidence: Available evidence does not support a physical interaction between NCK1 and GMPPA.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 409, "gene_symbol_1": "NCK1", "gene_symbol_2": "GMPPA", "detection_method": null}} +{"question_id": "PPIL1-0177", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: BTRC (B7Z3H4)\n\nProtein 2: PLK4 (O00444)\n Function: Serine/threonine-protein kinase that plays a central role in centriole duplication. Able to trigger procentriole formation on the surface of the parental centriole cylinder, leading to the recruitment\n Location: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole\n\nEvidence: Available evidence does not support a physical interaction between BTRC and PLK4.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 190, "gene_symbol_1": "BTRC", "gene_symbol_2": "PLK4", "detection_method": null}} +{"question_id": "PPIL1-0178", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: NDN (A0A0S2Z442)\n\nProtein 2: FXR2 (P51116)\n Function: mRNA-binding protein that acts as a regulator of mRNAs translation and/or stability, and which is required for adult hippocampal neurogenesis (By similarity). Specifically binds to AU-rich elements (A\n Location: Cytoplasm, Cytoplasmic ribonucleoprotein granule\n\nEvidence: Available evidence does not support a physical interaction between NDN and FXR2.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 478, "gene_symbol_1": "NDN", "gene_symbol_2": "FXR2", "detection_method": null}} +{"question_id": "PPIL1-0179", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: BEND5 (Q7L4P6)\n Function: Acts as a transcriptional repressor (PubMed:23468431)\n\nProtein 2: ZGPAT (Q8N5A5)\n Function: Transcription repressor that specifically binds the 5'-GGAG[GA]A[GA]A-3' consensus sequence. Represses transcription by recruiting the chromatin multiprotein complex NuRD to target promoters. Negative\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between BEND5 and ZGPAT.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 646, "gene_symbol_1": "BEND5", "gene_symbol_2": "ZGPAT", "detection_method": null}} +{"question_id": "PPIL1-0180", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: KIAA0907 (A0A0S2Z5A4)\n Function: RNA-binding protein involved in pre-mRNA splicing. Interacts with the PRP19C/Prp19 complex/NTC/Nineteen complex which is part of the spliceosome. Involved in regulating splice site selection. Binds pr\n\nProtein 2: PUF60 (Q9UHX1)\n Function: DNA- and RNA-binding protein, involved in several nuclear processes such as pre-mRNA splicing, apoptosis and transcription regulation. In association with FUBP1 regulates MYC transcription at the P2 p\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between KIAA0907 and PUF60.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 368, "gene_symbol_1": "KIAA0907", "gene_symbol_2": "PUF60", "detection_method": null}} +{"question_id": "PPIL1-0181", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: PPP3R1 (P63098)\n Function: Regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity\n Location: Cytoplasm, cytosol\n\nProtein 2: PPP3CA (Q08209)\n Function: Calcium-dependent, calmodulin-stimulated protein phosphatase which plays an essential role in the transduction of intracellular Ca(2+)-mediated signals (PubMed:15671020, PubMed:18838687, PubMed:191541\n Location: Cytoplasm\n\nEvidence: Available evidence does not support a physical interaction between PPP3R1 and PPP3CA.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 491, "gene_symbol_1": "PPP3R1", "gene_symbol_2": "PPP3CA", "detection_method": null}} +{"question_id": "PPIL1-0182", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: ABI2 (Q9NYB9)\n Function: Regulator of actin cytoskeleton dynamics underlying cell motility and adhesion. Functions as a component of the WAVE complex, which activates actin nucleating machinery Arp2/3 to drive lamellipodia fo\n Location: Cytoplasm\n\nProtein 2: ADAM29 (Q9UKF5)\n Function: May be involved in spermatogenesis and fertilization. Seems to be a non catalytic metalloprotease-like protein\n Location: Membrane\n\nEvidence: Available evidence does not support a physical interaction between ABI2 and ADAM29.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 29, "gene_symbol_1": "ABI2", "gene_symbol_2": "ADAM29", "detection_method": null}} +{"question_id": "PPIL1-0183", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: ABCF3 (A0A0S2Z645)\n\nProtein 2: TRIM27 (P14373)\n Function: E3 ubiquitin-protein ligase that mediates ubiquitination of various substrates and thereby plays a role in diffent processes including proliferation, innate immunity, apoptosis, immune response or aut\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between ABCF3 and TRIM27.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 128, "gene_symbol_1": "ABCF3", "gene_symbol_2": "TRIM27", "detection_method": null}} +{"question_id": "PPIL1-0184", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: MTUS2 (Q5JR59)\n Function: Binds microtubules. Together with MAPRE1 may target the microtubule depolymerase KIF2C to the plus-end of microtubules. May regulate the dynamics of microtubules at their growing distal tip\n Location: Cytoplasm, cytoskeleton\n\nProtein 2: TEAD4 (A0A0S2Z4E5)\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between MTUS2 and TEAD4.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 613, "gene_symbol_1": "MTUS2", "gene_symbol_2": "TEAD4", "detection_method": null}} +{"question_id": "PPIL1-0185", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: AXIN1 (A0A0S2Z4M1)\n Location: Cell membrane\n\nProtein 2: CARD9 (Q9H257)\n Function: Adapter protein that plays a key role in innate immune response against fungi by forming signaling complexes downstream of C-type lectin receptors (PubMed:26961233, PubMed:33558980). CARD9-mediated si\n Location: Cytoplasm\n\nEvidence: Available evidence does not support a physical interaction between AXIN1 and CARD9.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 153, "gene_symbol_1": "AXIN1", "gene_symbol_2": "CARD9", "detection_method": null}} +{"question_id": "PPIL1-0186", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: IKZF3 (Q9UKT9)\n Function: Transcription factor that plays an important role in the regulation of lymphocyte differentiation. Plays an essential role in regulation of B-cell differentiation, proliferation and maturation to an e\n Location: Nucleus\n\nProtein 2: AKAP10 (A0A0S2Z5A1)\n\nEvidence: Available evidence does not support a physical interaction between IKZF3 and AKAP10.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 158, "gene_symbol_1": "IKZF3", "gene_symbol_2": "AKAP10", "detection_method": null}} +{"question_id": "PPIL1-0187", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: ZKSCAN4 (Q969J2)\n Function: May be involved in the transcriptional activation of MDM2 and EP300 genes\n Location: Nucleus\n\nProtein 2: ZNF483 (Q6P088)\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between ZKSCAN4 and ZNF483.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 649, "gene_symbol_1": "ZKSCAN4", "gene_symbol_2": "ZNF483", "detection_method": null}} +{"question_id": "PPIL1-0188", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: IRAK1 (P51617)\n Function: Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly\n Location: Cytoplasm\n\nProtein 2: PELI3 (Q8N2H9)\n Function: E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins (PubMed:12874243, PubMed:17675297). Involved in the TLR and IL-1 signaling pathways via interaction\n\nEvidence: Available evidence does not support a physical interaction between IRAK1 and PELI3.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 22, "gene_symbol_1": "IRAK1", "gene_symbol_2": "PELI3", "detection_method": null}} +{"question_id": "PPIL1-0189", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: GNMT (A0A0S2Z594)\n Location: Cytoplasm\n\nProtein 2: GNMT (Q14749)\n Function: Catalyzes the methylation of glycine by using S-adenosylmethionine (AdoMet) to form N-methylglycine (sarcosine) with the concomitant production of S-adenosylhomocysteine (AdoHcy), a reaction regulated\n Location: Cytoplasm\n\nEvidence: Available evidence does not support a physical interaction between GNMT and GNMT.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 292, "gene_symbol_1": "GNMT", "gene_symbol_2": "GNMT", "detection_method": null}} +{"question_id": "PPIL1-0190", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: KRAS (P01116)\n Function: Ras proteins bind GDP/GTP and possess intrinsic GTPase activity (PubMed:20949621, PubMed:39809765). Plays an important role in the regulation of cell proliferation (PubMed:22711838, PubMed:23698361). \n Location: Cell membrane\n\nProtein 2: PIP4K2C (Q8TBX8)\n Function: Phosphatidylinositol 5-phosphate 4-kinase with low enzymatic activity. May be a GTP sensor, has higher GTP-dependent kinase activity than ATP-dependent kinase activity. PIP4Ks negatively regulate insu\n Location: Endoplasmic reticulum\n\nEvidence: Available evidence does not support a physical interaction between KRAS and PIP4K2C.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 779, "gene_symbol_1": "KRAS", "gene_symbol_2": "PIP4K2C", "detection_method": null}} +{"question_id": "PPIL1-0191", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: TERT (O14746)\n Function: Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. Active in progenitor and cancer cells. Inactive, or very low activity, in normal somati\n Location: Nucleus, nucleolus\n\nProtein 2: PML (P29590)\n Function: Functions via its association with PML-nuclear bodies (PML-NBs) in a wide range of important cellular processes, including tumor suppression, transcriptional regulation, apoptosis, senescence, DNA dam\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between TERT and PML.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 97, "gene_symbol_1": "TERT", "gene_symbol_2": "PML", "detection_method": null}} +{"question_id": "PPIL1-0192", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: TXNRD1 (Q16881)\n Function: Reduces disulfide protein thioredoxin (Trx) to its dithiol-containing form (PubMed:8577704). Homodimeric flavoprotein involved in the regulation of cellular redox reactions, growth and differentiation\n Location: Cytoplasm\n\nProtein 2: THRA (P10827)\n Function: Nuclear hormone receptor that can act as a repressor or activator of transcription. High affinity receptor for thyroid hormones, including triiodothyronine and thyroxine\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between TXNRD1 and THRA.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "gold", "result_id": 109, "gene_symbol_1": "TXNRD1", "gene_symbol_2": "THRA", "detection_method": null}} +{"question_id": "PPIL1-0193", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: NDN (A0A0S2Z3Z2)\n\nProtein 2: APBB1IP (Q7Z5R6)\n Function: Appears to function in the signal transduction from Ras activation to actin cytoskeletal remodeling. Suppresses insulin-induced promoter activities through AP1 and SRE. Mediates Rap1-induced adhesion\n Location: Cell membrane\n\nEvidence: Available evidence does not support a physical interaction between NDN and APBB1IP.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 471, "gene_symbol_1": "NDN", "gene_symbol_2": "APBB1IP", "detection_method": null}} +{"question_id": "PPIL1-0194", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: PRR20E (P86478)\n\nProtein 2: BANP (B4DE54)\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between PRR20E and BANP.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 170, "gene_symbol_1": "PRR20E", "gene_symbol_2": "BANP", "detection_method": null}} +{"question_id": "PPIL1-0195", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: CCDC172 (P0C7W6)\n Location: Cytoplasm\n\nProtein 2: TEAD4 (A0A0S2Z4F2)\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between CCDC172 and TEAD4.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 589, "gene_symbol_1": "CCDC172", "gene_symbol_2": "TEAD4", "detection_method": null}} +{"question_id": "PPIL1-0196", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: KRT18 (P05783)\n Function: Required for the formation of KRT8/KRT18 filaments that are involved in ARHGEF40-mediated actin stress fiber formation and tensional force-induced stress fiber formation and reinforcement (PubMed:2682\n Location: Nucleus matrix\n\nProtein 2: PSTPIP2 (Q9H939)\n Function: Binds to F-actin. May be involved in regulation of the actin cytoskeleton (By similarity)\n Location: Cytoplasm\n\nEvidence: Available evidence does not support a physical interaction between KRT18 and PSTPIP2.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 514, "gene_symbol_1": "KRT18", "gene_symbol_2": "PSTPIP2", "detection_method": null}} +{"question_id": "PPIL1-0197", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: HNRNPK (P61978)\n Function: One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich seq\n Location: Cytoplasm\n\nProtein 2: ZNF688 (A0A0S2Z6P0)\n\nEvidence: Available evidence does not support a physical interaction between HNRNPK and ZNF688.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 742, "gene_symbol_1": "HNRNPK", "gene_symbol_2": "ZNF688", "detection_method": null}} +{"question_id": "PPIL1-0198", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: NCK1 (P16333)\n Function: Adapter protein which associates with tyrosine-phosphorylated growth factor receptors, such as KDR and PDGFRB, or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting\n Location: Cytoplasm\n\nProtein 2: FASLG (P48023)\n Function: Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells (PubMed:26334989, PubMed:9228058). Involved in cytotoxic T-cell-mediated apoptosis, natural killer cell-m\n Location: Cell membrane\n\nEvidence: Available evidence does not support a physical interaction between NCK1 and FASLG.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 403, "gene_symbol_1": "NCK1", "gene_symbol_2": "FASLG", "detection_method": null}} +{"question_id": "PPIL1-0199", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: NDN (A0A0S2Z442)\n\nProtein 2: EEF1AKMT1 (Q8WVE0)\n Function: Protein N-lysine methyltransferase that selectively catalyzes the trimethylation of EEF1A at 'Lys-79'\n Location: Cytoplasm\n\nEvidence: Available evidence does not support a physical interaction between NDN and EEF1AKMT1.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 429, "gene_symbol_1": "NDN", "gene_symbol_2": "EEF1AKMT1", "detection_method": null}} +{"question_id": "PPIL1-0200", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: KRT31 (Q15323)\n\nProtein 2: GAD1 (Q99259)\n Function: Catalyzes the synthesis of the inhibitory neurotransmitter gamma-aminobutyric acid (GABA) with pyridoxal 5'-phosphate as cofactor\n\nEvidence: Available evidence does not support a physical interaction between KRT31 and GAD1.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 284, "gene_symbol_1": "KRT31", "gene_symbol_2": "GAD1", "detection_method": null}} +{"question_id": "PPIL1-0201", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: GKAP1 (Q5VSY0)\n Function: Regulates insulin-dependent IRS1 tyrosine phosphorylation in adipocytes by modulating the availability of IRS1 to IR tyrosine kinase. Its association with IRS1 is required for insulin-induced transloc\n Location: Golgi apparatus\n\nProtein 2: NDN (A0A0S2Z442)\n\nEvidence: Available evidence does not support a physical interaction between GKAP1 and NDN.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 479, "gene_symbol_1": "GKAP1", "gene_symbol_2": "NDN", "detection_method": null}} +{"question_id": "PPIL1-0202", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: CRX (O43186)\n Function: Transcription factor that binds and transactivates the sequence 5'-TAATC[CA]-3' which is found upstream of several photoreceptor-specific genes, including the opsin genes. Acts synergistically with ot\n Location: Nucleus\n\nProtein 2: ZNF688 (A0A0S2Z5X4)\n\nEvidence: Available evidence does not support a physical interaction between CRX and ZNF688.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 681, "gene_symbol_1": "CRX", "gene_symbol_2": "ZNF688", "detection_method": null}} +{"question_id": "PPIL1-0203", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: AGTRAP (Q6RW13)\n Function: Appears to be a negative regulator of type-1 angiotensin II receptor-mediated signaling by regulating receptor internalization as well as mechanism of receptor desensitization such as phosphorylation.\n Location: Endoplasmic reticulum membrane\n\nProtein 2: FGA (Q8WW76)\n Location: Secreted\n\nEvidence: Available evidence does not support a physical interaction between AGTRAP and FGA.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 286, "gene_symbol_1": "AGTRAP", "gene_symbol_2": "FGA", "detection_method": null}} +{"question_id": "PPIL1-0204", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: CEP76 (Q8TAP6)\n Function: Centrosomal protein involved in regulation of centriole duplication. Required to limit centriole duplication to once per cell cycle by preventing centriole reduplication\n Location: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome\n\nProtein 2: NME5 (P56597)\n Function: Functions as part of axonemal radial spoke complexes, which play an important part in the motility of sperm and cilia (By similarity). In vitro, does not display nucleoside diphosphate kinase (NDPK) a\n Location: Cell projection, cilium\n\nEvidence: Available evidence does not support a physical interaction between CEP76 and NME5.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 497, "gene_symbol_1": "CEP76", "gene_symbol_2": "NME5", "detection_method": null}} +{"question_id": "PPIL1-0205", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: REEP6 (Q96HR9)\n Function: Required for correct function and survival of retinal photoreceptors (PubMed:27889058). Required for retinal development (By similarity). In rod photoreceptors, facilitates stability and/or traffickin\n Location: Endoplasmic reticulum membrane\n\nProtein 2: NDRG4 (Q9ULP0)\n Function: Contributes to the maintenance of intracerebral BDNF levels within the normal range, which is necessary for the preservation of spatial learning and the resistance to neuronal cell death caused by isc\n Location: Cytoplasm, cytosol\n\nEvidence: Available evidence does not support a physical interaction between REEP6 and NDRG4.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 469, "gene_symbol_1": "REEP6", "gene_symbol_2": "NDRG4", "detection_method": null}} +{"question_id": "PPIL1-0206", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: TRIM27 (P14373)\n Function: E3 ubiquitin-protein ligase that mediates ubiquitination of various substrates and thereby plays a role in diffent processes including proliferation, innate immunity, apoptosis, immune response or aut\n Location: Nucleus\n\nProtein 2: TEAD4 (A0A0S2Z4E5)\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between TRIM27 and TEAD4.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 587, "gene_symbol_1": "TRIM27", "gene_symbol_2": "TEAD4", "detection_method": null}} +{"question_id": "PPIL1-0207", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: YWHAZ (P63104)\n Function: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways (PubMed:14578935, PubMed:15071501, PubMed:15644438, PubMed:16376338, PubMed:16959763\n Location: Cytoplasm\n\nProtein 2: SMARCB1 (Q12824)\n Function: Core component of the BAF (hSWI/SNF) complex. This ATP-dependent chromatin-remodeling complex plays important roles in cell proliferation and differentiation, in cellular antiviral activities and inhi\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between YWHAZ and SMARCB1.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 86, "gene_symbol_1": "YWHAZ", "gene_symbol_2": "SMARCB1", "detection_method": null}} +{"question_id": "PPIL1-0208", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: MICU2 (Q8IYU8)\n Function: Calcium sensor of the mitochondrial calcium uniporter (MCU) channel, which senses calcium level via its EF-hand domains (PubMed:24503055, PubMed:24560927, PubMed:26903221, PubMed:28615291, PubMed:3069\n Location: Mitochondrion intermembrane space\n\nProtein 2: GPRASP2 (Q96D09)\n Function: May play a role in regulation of a variety of G-protein coupled receptors\n\nEvidence: Available evidence does not support a physical interaction between MICU2 and GPRASP2.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 248, "gene_symbol_1": "MICU2", "gene_symbol_2": "GPRASP2", "detection_method": null}} +{"question_id": "PPIL1-0209", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: TFCP2 (Q12800)\n Function: Binds a variety of cellular and viral promoters including fibrinogen, alpha-globin, SV40 and HIV-1 promoters. Activation of the alpha-globin promoter in erythroid cells is via synergistic interaction \n Location: Nucleus\n\nProtein 2: RXRB (A0A0S2Z4M5)\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between TFCP2 and RXRB.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 550, "gene_symbol_1": "TFCP2", "gene_symbol_2": "RXRB", "detection_method": null}} +{"question_id": "PPIL1-0210", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: ABCF3 (Q9NUQ8)\n Function: Displays an antiviral effect against flaviviruses such as west Nile virus (WNV) in the presence of OAS1B\n\nProtein 2: PSME3 (P61289)\n Function: Subunit of the 11S REG-gamma (also called PA28-gamma) proteasome regulator, a doughnut-shaped homoheptamer which associates with the proteasome. 11S REG-gamma activates the trypsin-like catalytic subu\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between ABCF3 and PSME3.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 120, "gene_symbol_1": "ABCF3", "gene_symbol_2": "PSME3", "detection_method": null}} +{"question_id": "PPIL1-0211", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: TEAD4 (A0A0S2Z4E5)\n Location: Nucleus\n\nProtein 2: HOMEZ (Q8IX15)\n Function: May function as a transcriptional regulator\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between TEAD4 and HOMEZ.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 592, "gene_symbol_1": "TEAD4", "gene_symbol_2": "HOMEZ", "detection_method": null}} +{"question_id": "PPIL1-0212", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: USHBP1 (Q8N6Y0)\n\nProtein 2: FANCG (A0A0S2Z3S1)\n Function: DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. May be implicated in interstrand DNA cross-link repair and in the maintenance of normal chromosome \n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between USHBP1 and FANCG.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 282, "gene_symbol_1": "USHBP1", "gene_symbol_2": "FANCG", "detection_method": null}} +{"question_id": "PPIL1-0213", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: ATXN1 (P54253)\n Function: Chromatin-binding factor that repress Notch signaling in the absence of Notch intracellular domain by acting as a CBF1 corepressor. Binds to the HEY promoter and might assist, along with NCOR2, RBPJ-m\n Location: Cytoplasm\n\nProtein 2: BATF2 (Q8N1L9)\n Function: AP-1 family transcription factor that controls the differentiation of lineage-specific cells in the immune system. Following infection, participates in the differentiation of CD8(+) thymic conventiona\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between ATXN1 and BATF2.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "gold", "result_id": 45, "gene_symbol_1": "ATXN1", "gene_symbol_2": "BATF2", "detection_method": null}} +{"question_id": "PPIL1-0214", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: CMTM5 (Q96DZ9)\n Location: Membrane\n\nProtein 2: NDRG4 (Q9ULP0)\n Function: Contributes to the maintenance of intracerebral BDNF levels within the normal range, which is necessary for the preservation of spatial learning and the resistance to neuronal cell death caused by isc\n Location: Cytoplasm, cytosol\n\nEvidence: Available evidence does not support a physical interaction between CMTM5 and NDRG4.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 485, "gene_symbol_1": "CMTM5", "gene_symbol_2": "NDRG4", "detection_method": null}} +{"question_id": "PPIL1-0215", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: VAC14 (Q08AM6)\n Function: Scaffold protein component of the PI(3,5)P2 regulatory complex which regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Pentamerizes into a star-shaped\n Location: Endosome membrane\n\nProtein 2: ZCWPW1 (A0A0S2Z5E1)\n\nEvidence: Available evidence does not support a physical interaction between VAC14 and ZCWPW1.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 640, "gene_symbol_1": "VAC14", "gene_symbol_2": "ZCWPW1", "detection_method": null}} +{"question_id": "PPIL1-0216", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: WASL (O00401)\n Function: Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex (PubMed:16767080, PubMed:19366662, PubMed:19487689, PubMed:22847007, PubMed:22921828, PubMed:9422512).\n Location: Cytoplasm, cytoskeleton\n\nProtein 2: NCK1 (A0A0S2Z4E4)\n\nEvidence: Available evidence does not support a physical interaction between WASL and NCK1.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 418, "gene_symbol_1": "WASL", "gene_symbol_2": "NCK1", "detection_method": null}} +{"question_id": "PPIL1-0217", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: IKZF3 (Q9UKT9)\n Function: Transcription factor that plays an important role in the regulation of lymphocyte differentiation. Plays an essential role in regulation of B-cell differentiation, proliferation and maturation to an e\n Location: Nucleus\n\nProtein 2: AKAP10 (A0A0S2Z551)\n\nEvidence: Available evidence does not support a physical interaction between IKZF3 and AKAP10.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 144, "gene_symbol_1": "IKZF3", "gene_symbol_2": "AKAP10", "detection_method": null}} +{"question_id": "PPIL1-0218", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: MEOX1 (P50221)\n Function: Mesodermal transcription factor that plays a key role in somitogenesis and is specifically required for sclerotome development. Required for maintenance of the sclerotome polarity and formation of the\n Location: Nucleus\n\nProtein 2: BANP (E9PJI6)\n\nEvidence: Available evidence does not support a physical interaction between MEOX1 and BANP.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 166, "gene_symbol_1": "MEOX1", "gene_symbol_2": "BANP", "detection_method": null}} +{"question_id": "PPIL1-0219", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: PIK3R1 (P27986)\n Function: Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the \n\nProtein 2: SYK (P43405)\n Function: Non-receptor tyrosine kinase which mediates signal transduction downstream of a variety of transmembrane receptors including classical immunoreceptors like the B-cell receptor (BCR). Regulates several\n Location: Cell membrane\n\nEvidence: Available evidence does not support a physical interaction between PIK3R1 and SYK.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 33, "gene_symbol_1": "PIK3R1", "gene_symbol_2": "SYK", "detection_method": null}} +{"question_id": "PPIL1-0220", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: PIMREG (Q9BSJ6)\n Function: During mitosis, may play a role in the control of metaphase-to-anaphase transition\n Location: Nucleus\n\nProtein 2: MAL2 (Q969L2)\n Function: Member of the machinery of polarized transport. Required for the indirect transcytotic route at the step of the egress of the transcytosing cargo from perinuclear endosomes in order for it to travel t\n Location: Cell membrane\n\nEvidence: Available evidence does not support a physical interaction between PIMREG and MAL2.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 272, "gene_symbol_1": "PIMREG", "gene_symbol_2": "MAL2", "detection_method": null}} +{"question_id": "PPIL1-0221", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: USHBP1 (Q8N6Y0)\n\nProtein 2: HGS (A0A0S2Z4Q4)\n Location: Cytoplasm\n\nEvidence: Available evidence does not support a physical interaction between USHBP1 and HGS.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 305, "gene_symbol_1": "USHBP1", "gene_symbol_2": "HGS", "detection_method": null}} +{"question_id": "PPIL1-0222", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: DMC1 (Q14565)\n Function: Participates in meiotic recombination, specifically in homologous strand assimilation, which is required for the resolution of meiotic double-strand breaks\n Location: Nucleus\n\nProtein 2: RAD51AP1 (Q96B01)\n Function: Structure-specific DNA-binding protein involved in DNA repair by promoting RAD51-mediated homologous recombination (PubMed:17996710, PubMed:17996711, PubMed:20871616, PubMed:25288561, PubMed:26323318)\n Location: Chromosome\n\nEvidence: Available evidence does not support a physical interaction between DMC1 and RAD51AP1.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "gold", "result_id": 82, "gene_symbol_1": "DMC1", "gene_symbol_2": "RAD51AP1", "detection_method": null}} +{"question_id": "PPIL1-0223", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: GAD1 (Q99259)\n Function: Catalyzes the synthesis of the inhibitory neurotransmitter gamma-aminobutyric acid (GABA) with pyridoxal 5'-phosphate as cofactor\n\nProtein 2: TRIP6 (Q15654)\n Function: Relays signals from the cell surface to the nucleus to weaken adherens junction and promote actin cytoskeleton reorganization and cell invasiveness. Involved in lysophosphatidic acid-induced cell adhe\n Location: Cytoplasm, cytoskeleton\n\nEvidence: Available evidence does not support a physical interaction between GAD1 and TRIP6.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 295, "gene_symbol_1": "GAD1", "gene_symbol_2": "TRIP6", "detection_method": null}} +{"question_id": "PPIL1-0224", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: RIPK1 (Q13546)\n Function: Serine-threonine kinase which is a key regulator of TNF-mediated apoptosis, necroptosis and inflammatory pathways (PubMed:17703191, PubMed:24144979, PubMed:31827280, PubMed:31827281, PubMed:32657447, \n Location: Cytoplasm\n\nProtein 2: CASP8 (Q14790)\n Function: Thiol protease that plays a key role in programmed cell death by acting as a molecular switch for apoptosis, necroptosis and pyroptosis, and is required to prevent tissue damage during embryonic devel\n Location: Cytoplasm\n\nEvidence: Available evidence does not support a physical interaction between RIPK1 and CASP8.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "gold", "result_id": 80, "gene_symbol_1": "RIPK1", "gene_symbol_2": "CASP8", "detection_method": null}} +{"question_id": "PPIL1-0225", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: NDN (A0A0S2Z3Y6)\n\nProtein 2: ZBTB7B (O15156)\n Function: Transcription regulator that acts as a key regulator of lineage commitment of immature T-cell precursors. Exerts distinct biological functions in the mammary epithelial cells and T cells in a tissue-s\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between NDN and ZBTB7B.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 454, "gene_symbol_1": "NDN", "gene_symbol_2": "ZBTB7B", "detection_method": null}} +{"question_id": "PPIL1-0226", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: GOLGA2 (Q08379)\n Function: Peripheral membrane component of the cis-Golgi stack that acts as a membrane skeleton that maintains the structure of the Golgi apparatus, and as a vesicle thether that facilitates vesicle fusion to t\n Location: Golgi apparatus, cis-Golgi network membrane\n\nProtein 2: RXRB (P28702)\n Function: Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression \n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between GOLGA2 and RXRB.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 532, "gene_symbol_1": "GOLGA2", "gene_symbol_2": "RXRB", "detection_method": null}} +{"question_id": "PPIL1-0227", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: NEFL (I6L9F6)\n Function: Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal interm\n Location: Cytoplasm\n\nProtein 2: CCDC172 (P0C7W6)\n Location: Cytoplasm\n\nEvidence: Available evidence does not support a physical interaction between NEFL and CCDC172.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 492, "gene_symbol_1": "NEFL", "gene_symbol_2": "CCDC172", "detection_method": null}} +{"question_id": "PPIL1-0228", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: HGS (A0A0S2Z4Q4)\n Location: Cytoplasm\n\nProtein 2: CEP63 (Q96MT8)\n Function: Required for normal spindle assembly (PubMed:21406398, PubMed:21983783, PubMed:26297806, PubMed:35793002). Plays a key role in mother-centriole-dependent centriole duplication; the function seems also\n Location: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome\n\nEvidence: Available evidence does not support a physical interaction between HGS and CEP63.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 301, "gene_symbol_1": "HGS", "gene_symbol_2": "CEP63", "detection_method": null}} +{"question_id": "PPIL1-0229", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: NCK2 (E7ERP6)\n\nProtein 2: CBLB (Q13191)\n Function: E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome (PubMed:2052\n Location: Cytoplasm\n\nEvidence: Available evidence does not support a physical interaction between NCK2 and CBLB.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 411, "gene_symbol_1": "NCK2", "gene_symbol_2": "CBLB", "detection_method": null}} +{"question_id": "PPIL1-0230", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: PCBP2 (Q15366)\n Function: Single-stranded nucleic acid binding protein that binds preferentially to oligo dC (PubMed:12414943, PubMed:7607214). Major cellular poly(rC)-binding protein (PubMed:12414943). Also binds poly(rU) (Pu\n Location: Nucleus\n\nProtein 2: QKI (Q96PU8)\n Function: RNA reader protein, which recognizes and binds specific RNAs, thereby regulating RNA metabolic processes, such as pre-mRNA splicing, circular RNA (circRNA) formation, mRNA export, mRNA stability and/o\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between PCBP2 and QKI.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "gold", "result_id": 50, "gene_symbol_1": "PCBP2", "gene_symbol_2": "QKI", "detection_method": null}} +{"question_id": "PPIL1-0231", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: ITGB2 (P05107)\n Function: Integrin ITGAL:ITGB2 is a receptor for ICAM1, ICAM2 and ICAM3 (PubMed:1676048, PubMed:23775590, PubMed:38195629). Integrin ITGAL:ITGB2 is also a receptor for the secreted form of ubiquitin-like protei\n Location: Cell membrane\n\nProtein 2: MAPK8IP1 (Q9UQF2)\n Function: The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. Requir\n Location: Cytoplasm\n\nEvidence: Available evidence does not support a physical interaction between ITGB2 and MAPK8IP1.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "gold", "result_id": 17, "gene_symbol_1": "ITGB2", "gene_symbol_2": "MAPK8IP1", "detection_method": null}} +{"question_id": "PPIL1-0232", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: RXRB (A0A0S2Z4M5)\n Location: Nucleus\n\nProtein 2: TADA3 (O75528)\n Function: Functions as a component of the PCAF complex. The PCAF complex is capable of efficiently acetylating histones in a nucleosomal context. The PCAF complex could be considered as the human version of the\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between RXRB and TADA3.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 555, "gene_symbol_1": "RXRB", "gene_symbol_2": "TADA3", "detection_method": null}} +{"question_id": "PPIL1-0233", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: TP53 (P04637)\n Function: Multifunctional transcription factor that induces cell cycle arrest, DNA repair or apoptosis upon binding to its target DNA sequence (PubMed:11025664, PubMed:12524540, PubMed:12810724, PubMed:15186775\n Location: Cytoplasm\n\nProtein 2: NDN (A0A0S2Z3Y6)\n\nEvidence: Available evidence does not support a physical interaction between TP53 and NDN.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 465, "gene_symbol_1": "TP53", "gene_symbol_2": "NDN", "detection_method": null}} +{"question_id": "PPIL1-0234", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: USHBP1 (Q8N6Y0)\n\nProtein 2: MXI1 (A0A0S2Z429)\n\nEvidence: Available evidence does not support a physical interaction between USHBP1 and MXI1.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 423, "gene_symbol_1": "USHBP1", "gene_symbol_2": "MXI1", "detection_method": null}} +{"question_id": "PPIL1-0235", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: ITGB1 (P05556)\n Function: Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta-1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha-1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-\n Location: Cell membrane\n\nProtein 2: RGS12 (O14924)\n Function: Regulates G protein-coupled receptor signaling cascades. Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound \n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between ITGB1 and RGS12.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "gold", "result_id": 16, "gene_symbol_1": "ITGB1", "gene_symbol_2": "RGS12", "detection_method": null}} +{"question_id": "PPIL1-0236", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: TTC12 (Q9H892)\n Function: Cytoplasmic protein that plays a role in the proper assembly of dynein arm complexes in motile cilia in both respiratory cells and sperm flagella\n Location: Cytoplasm\n\nProtein 2: MIPOL1 (A0A0S2Z5W4)\n\nEvidence: Available evidence does not support a physical interaction between TTC12 and MIPOL1.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 395, "gene_symbol_1": "TTC12", "gene_symbol_2": "MIPOL1", "detection_method": null}} +{"question_id": "PPIL1-0237", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: NCK1 (P16333)\n Function: Adapter protein which associates with tyrosine-phosphorylated growth factor receptors, such as KDR and PDGFRB, or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting\n Location: Cytoplasm\n\nProtein 2: SORBS3 (O60504)\n Function: Vinexin alpha isoform promotes up-regulation of actin stress fiber formation. Vinexin beta isoform plays a role in cell spreading and enhances the activation of JNK/SAPK in response to EGF stimulation\n Location: Cell junction\n\nEvidence: Available evidence does not support a physical interaction between NCK1 and SORBS3.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 427, "gene_symbol_1": "NCK1", "gene_symbol_2": "SORBS3", "detection_method": null}} +{"question_id": "PPIL1-0238", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: VAPB (O95292)\n Function: Endoplasmic reticulum (ER)-anchored protein that mediates the formation of contact sites between the ER and endosomes via interaction with FFAT motif-containing proteins such as STARD3 or WDR44 (PubMe\n Location: Endoplasmic reticulum membrane\n\nProtein 2: VAPA (Q9P0L0)\n Function: Endoplasmic reticulum (ER)-anchored protein that mediates the formation of contact sites between the ER and endosomes via interaction with FFAT motif-containing proteins such as STARD3 or WDR44 (PubMe\n Location: Endoplasmic reticulum membrane\n\nEvidence: Available evidence does not support a physical interaction between VAPB and VAPA.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 111, "gene_symbol_1": "VAPB", "gene_symbol_2": "VAPA", "detection_method": null}} +{"question_id": "PPIL1-0239", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: BANF1 (O75531)\n Function: Non-specific DNA-binding protein that plays key roles in mitotic nuclear reassembly, chromatin organization, DNA damage response, gene expression and intrinsic immunity against foreign DNA (PubMed:109\n Location: Nucleus\n\nProtein 2: ANKLE1 (Q8NAG6)\n Function: Structure-selective DNA endonuclease that specifically cleaves branched DNA (PubMed:22399800, PubMed:27245214, PubMed:32866453, PubMed:36825683, PubMed:37216589). Processes chromatin bridges during cy\n Location: Cytoplasm\n\nEvidence: Available evidence does not support a physical interaction between BANF1 and ANKLE1.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "gold", "result_id": 766, "gene_symbol_1": "BANF1", "gene_symbol_2": "ANKLE1", "detection_method": null}} +{"question_id": "PPIL1-0240", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: HNRNPK (P61978)\n Function: One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich seq\n Location: Cytoplasm\n\nProtein 2: RBM14 (Q96PK6)\n Function: Isoform 1 may function as a nuclear receptor coactivator, enhancing transcription through other coactivators such as NCOA6 and CITED1. Isoform 2, functions as a transcriptional repressor, modulating t\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between HNRNPK and RBM14.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 525, "gene_symbol_1": "HNRNPK", "gene_symbol_2": "RBM14", "detection_method": null}} +{"question_id": "PPIL1-0241", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: MTUS2 (Q5JR59)\n Function: Binds microtubules. Together with MAPRE1 may target the microtubule depolymerase KIF2C to the plus-end of microtubules. May regulate the dynamics of microtubules at their growing distal tip\n Location: Cytoplasm, cytoskeleton\n\nProtein 2: KRT6A (P02538)\n Function: Epidermis-specific type I keratin involved in wound healing. Involved in the activation of follicular keratinocytes after wounding, while it does not play a major role in keratinocyte proliferation or\n\nEvidence: Available evidence does not support a physical interaction between MTUS2 and KRT6A.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 372, "gene_symbol_1": "MTUS2", "gene_symbol_2": "KRT6A", "detection_method": null}} +{"question_id": "PPIL1-0242", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: E2F1 (Q01094)\n Function: Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved i\n Location: Nucleus\n\nProtein 2: DDB2 (Q92466)\n Function: Protein, which is both involved in DNA repair and protein ubiquitination, as part of the UV-DDB complex and DCX (DDB1-CUL4-X-box) complexes, respectively (PubMed:10882109, PubMed:11278856, PubMed:1170\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between E2F1 and DDB2.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 66, "gene_symbol_1": "E2F1", "gene_symbol_2": "DDB2", "detection_method": null}} +{"question_id": "PPIL1-0243", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: HMG20A (Q9NP66)\n Function: Plays a role in neuronal differentiation as chromatin-associated protein. Acts as inhibitor of HMG20B. Overcomes the repressive effects of the neuronal silencer REST and induces the activation of neur\n Location: Nucleus\n\nProtein 2: DCTN4 (Q9UJW0)\n Function: Part of the dynactin complex that activates the molecular motor dynein for ultra-processive transport along microtubules\n Location: Cytoplasm, cytoskeleton\n\nEvidence: Available evidence does not support a physical interaction between HMG20A and DCTN4.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 217, "gene_symbol_1": "HMG20A", "gene_symbol_2": "DCTN4", "detection_method": null}} +{"question_id": "PPIL1-0244", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: AGTRAP (Q6RW13)\n Function: Appears to be a negative regulator of type-1 angiotensin II receptor-mediated signaling by regulating receptor internalization as well as mechanism of receptor desensitization such as phosphorylation.\n Location: Endoplasmic reticulum membrane\n\nProtein 2: PIMREG (Q9BSJ6)\n Function: During mitosis, may play a role in the control of metaphase-to-anaphase transition\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between AGTRAP and PIMREG.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 250, "gene_symbol_1": "AGTRAP", "gene_symbol_2": "PIMREG", "detection_method": null}} +{"question_id": "PPIL1-0245", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: FOXP2 (O15409)\n Function: Transcriptional repressor that may play a role in the specification and differentiation of lung epithelium. May also play a role in developing neural, gastrointestinal and cardiovascular tissues. Can \n Location: Nucleus\n\nProtein 2: TBR1 (Q16650)\n Function: Transcriptional repressor involved in multiple aspects of cortical development, including neuronal migration, laminar and areal identity, and axonal projection (PubMed:25232744, PubMed:30250039). As t\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between FOXP2 and TBR1.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 757, "gene_symbol_1": "FOXP2", "gene_symbol_2": "TBR1", "detection_method": null}} +{"question_id": "PPIL1-0246", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: NFYB (P25208)\n Function: Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes. NF-Y can function as bot\n Location: Nucleus\n\nProtein 2: HDAC4 (P56524)\n Function: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an importa\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between NFYB and HDAC4.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 19, "gene_symbol_1": "NFYB", "gene_symbol_2": "HDAC4", "detection_method": null}} +{"question_id": "PPIL1-0247", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: REL (Q04864)\n Function: Proto-oncogene that may play a role in differentiation and lymphopoiesis. NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological \n Location: Nucleus\n\nProtein 2: ACBD4 (A0A0S2Z5W7)\n\nEvidence: Available evidence does not support a physical interaction between REL and ACBD4.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 115, "gene_symbol_1": "REL", "gene_symbol_2": "ACBD4", "detection_method": null}} +{"question_id": "PPIL1-0248", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: PIMREG (Q9BSJ6)\n Function: During mitosis, may play a role in the control of metaphase-to-anaphase transition\n Location: Nucleus\n\nProtein 2: CEP70 (Q8NHQ1)\n Function: Plays a role in the organization of both preexisting and nascent microtubules in interphase cells. During mitosis, required for the organization and orientation of the mitotic spindle\n Location: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome\n\nEvidence: Available evidence does not support a physical interaction between PIMREG and CEP70.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 266, "gene_symbol_1": "PIMREG", "gene_symbol_2": "CEP70", "detection_method": null}} +{"question_id": "PPIL1-0249", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: NDN (A0A0S2Z442)\n\nProtein 2: MKRN2 (Q9H000)\n Function: E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins (By similarity). Promotes the polyubiquitination and proteasome-dependent degradation of RELA/p65, \n Location: Cytoplasm\n\nEvidence: Available evidence does not support a physical interaction between NDN and MKRN2.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 446, "gene_symbol_1": "NDN", "gene_symbol_2": "MKRN2", "detection_method": null}} +{"question_id": "PPIL1-0250", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: SPAG5 (Q96R06)\n Function: Essential component of the mitotic spindle required for normal chromosome segregation and progression into anaphase (PubMed:11724960, PubMed:12356910, PubMed:27462074). Required for chromosome alignme\n Location: Cytoplasm\n\nProtein 2: ZNF688 (A0A0S2Z6P0)\n\nEvidence: Available evidence does not support a physical interaction between SPAG5 and ZNF688.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 736, "gene_symbol_1": "SPAG5", "gene_symbol_2": "ZNF688", "detection_method": null}} +{"question_id": "PPIL1-0251", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: HRAS (P01112)\n Function: Involved in the activation of Ras protein signal transduction (PubMed:22821884). Ras proteins bind GDP/GTP and possess intrinsic GTPase activity (PubMed:12740440, PubMed:14500341, PubMed:9020151)\n Location: Cell membrane\n\nProtein 2: FYN (P06241)\n Function: Non-receptor tyrosine-protein kinase that plays a role in many biological processes including regulation of cell growth and survival, cell adhesion, integrin-mediated signaling, cytoskeletal remodelin\n Location: Cytoplasm\n\nEvidence: Available evidence does not support a physical interaction between HRAS and FYN.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "gold", "result_id": 21, "gene_symbol_1": "HRAS", "gene_symbol_2": "FYN", "detection_method": null}} +{"question_id": "PPIL1-0252", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: DNM1L (O00429)\n Function: Functions in mitochondrial and peroxisomal division (PubMed:11514614, PubMed:12499366, PubMed:17301055, PubMed:17460227, PubMed:17553808, PubMed:18695047, PubMed:18838687, PubMed:19342591, PubMed:1941\n Location: Cytoplasm, cytosol\n\nProtein 2: PGAM5 (Q96HS1)\n Function: Mitochondrial serine/threonine phosphatase that dephosphorylates various substrates and thus plays a role in different biological processes including cellular senescence or mitophagy (PubMed:24746696,\n Location: Mitochondrion outer membrane\n\nEvidence: Available evidence does not support a physical interaction between DNM1L and PGAM5.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 83, "gene_symbol_1": "DNM1L", "gene_symbol_2": "PGAM5", "detection_method": null}} +{"question_id": "PPIL1-0253", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: GPRASP2 (Q96D09)\n Function: May play a role in regulation of a variety of G-protein coupled receptors\n\nProtein 2: ZNF688 (P0C7X2)\n Function: May be involved in transcriptional regulation\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between GPRASP2 and ZNF688.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 745, "gene_symbol_1": "GPRASP2", "gene_symbol_2": "ZNF688", "detection_method": null}} +{"question_id": "PPIL1-0254", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: TRIM27 (P14373)\n Function: E3 ubiquitin-protein ligase that mediates ubiquitination of various substrates and thereby plays a role in diffent processes including proliferation, innate immunity, apoptosis, immune response or aut\n Location: Nucleus\n\nProtein 2: PIMREG (Q9BSJ6)\n Function: During mitosis, may play a role in the control of metaphase-to-anaphase transition\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between TRIM27 and PIMREG.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 255, "gene_symbol_1": "TRIM27", "gene_symbol_2": "PIMREG", "detection_method": null}} +{"question_id": "PPIL1-0255", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: ACBD4 (Q8NC06)\n Function: Binds medium- and long-chain acyl-CoA esters and may function as an intracellular carrier of acyl-CoA esters\n\nProtein 2: CRX (O43186)\n Function: Transcription factor that binds and transactivates the sequence 5'-TAATC[CA]-3' which is found upstream of several photoreceptor-specific genes, including the opsin genes. Acts synergistically with ot\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between ACBD4 and CRX.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 123, "gene_symbol_1": "ACBD4", "gene_symbol_2": "CRX", "detection_method": null}} +{"question_id": "PPIL1-0256", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: DYX1C1 (A0A0S2Z5X9)\n Location: Cell projection, neuron projection\n\nProtein 2: CRACR2B (Q8N4Y2)\n Function: Plays a role in store-operated Ca(2+) entry (SOCE)\n\nEvidence: Available evidence does not support a physical interaction between DYX1C1 and CRACR2B.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 259, "gene_symbol_1": "DYX1C1", "gene_symbol_2": "CRACR2B", "detection_method": null}} +{"question_id": "PPIL1-0257", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: FKBP8 (Q14318)\n Function: Constitutively inactive PPiase, which becomes active when bound to calmodulin and calcium. Seems to act as a chaperone for BCL2, targets it to the mitochondria and modulates its phosphorylation state.\n Location: Mitochondrion\n\nProtein 2: MYLK (Q15746)\n Function: Calcium/calmodulin-dependent myosin light chain kinase implicated in smooth muscle contraction via phosphorylation of myosin light chains (MLC). Also regulates actin-myosin interaction through a non-k\n Location: Cytoplasm\n\nEvidence: Available evidence does not support a physical interaction between FKBP8 and MYLK.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 775, "gene_symbol_1": "FKBP8", "gene_symbol_2": "MYLK", "detection_method": null}} +{"question_id": "PPIL1-0258", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: TP53 (P04637)\n Function: Multifunctional transcription factor that induces cell cycle arrest, DNA repair or apoptosis upon binding to its target DNA sequence (PubMed:11025664, PubMed:12524540, PubMed:12810724, PubMed:15186775\n Location: Cytoplasm\n\nProtein 2: DDX5 (P17844)\n Function: Involved in the alternative regulation of pre-mRNA splicing; its RNA helicase activity is necessary for increasing tau exon 10 inclusion and occurs in a RBM4-dependent manner. Binds to the tau pre-mRN\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between TP53 and DDX5.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "gold", "result_id": 72, "gene_symbol_1": "TP53", "gene_symbol_2": "DDX5", "detection_method": null}} +{"question_id": "PPIL1-0259", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: APBA1 (Q02410)\n Function: Putative function in synaptic vesicle exocytosis by binding to Munc18-1, an essential component of the synaptic vesicle exocytotic machinery. May modulate processing of the amyloid-beta precursor prot\n Location: Cytoplasm\n\nProtein 2: RAB6A (P20340)\n Function: The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes (PubMed:25962623). Rabs cycle between an inactiv\n Location: Golgi apparatus membrane\n\nEvidence: Available evidence does not support a physical interaction between APBA1 and RAB6A.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 101, "gene_symbol_1": "APBA1", "gene_symbol_2": "RAB6A", "detection_method": null}} +{"question_id": "PPIL1-0260", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: CDK16 (Q00536)\n Function: Protein kinase that plays a role in vesicle-mediated transport processes and exocytosis. Regulates GH1 release by brain neurons. Phosphorylates NSF, and thereby regulates NSF oligomerization. Required\n Location: Cytoplasm\n\nProtein 2: CCNY (Q8ND76)\n Function: Positive regulatory subunit of the cyclin-dependent kinases CDK14/PFTK1 and CDK16. Acts as a cell-cycle regulator of Wnt signaling pathway during G2/M phase by recruiting CDK14/PFTK1 to the plasma mem\n Location: Cell membrane\n\nEvidence: Available evidence does not support a physical interaction between CDK16 and CCNY.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 753, "gene_symbol_1": "CDK16", "gene_symbol_2": "CCNY", "detection_method": null}} +{"question_id": "PPIL1-0261", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: SIAH1 (Q8IUQ4)\n Function: E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins (PubMed:14506261, PubMed:14645235, PubMed:14654780, PubMed:15064394, PubMed:16085652,\n Location: Cytoplasm\n\nProtein 2: UPP2 (A0A0S2Z639)\n\nEvidence: Available evidence does not support a physical interaction between SIAH1 and UPP2.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 625, "gene_symbol_1": "SIAH1", "gene_symbol_2": "UPP2", "detection_method": null}} +{"question_id": "PPIL1-0262", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: TERT (O14746)\n Function: Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. Active in progenitor and cancer cells. Inactive, or very low activity, in normal somati\n Location: Nucleus, nucleolus\n\nProtein 2: PML (P29590)\n Function: Functions via its association with PML-nuclear bodies (PML-NBs) in a wide range of important cellular processes, including tumor suppression, transcriptional regulation, apoptosis, senescence, DNA dam\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between TERT and PML.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 92, "gene_symbol_1": "TERT", "gene_symbol_2": "PML", "detection_method": null}} +{"question_id": "PPIL1-0263", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: CRX (O43186)\n Function: Transcription factor that binds and transactivates the sequence 5'-TAATC[CA]-3' which is found upstream of several photoreceptor-specific genes, including the opsin genes. Acts synergistically with ot\n Location: Nucleus\n\nProtein 2: HGS (A0A0S2Z4Q4)\n Location: Cytoplasm\n\nEvidence: Available evidence does not support a physical interaction between CRX and HGS.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 290, "gene_symbol_1": "CRX", "gene_symbol_2": "HGS", "detection_method": null}} +{"question_id": "PPIL1-0264", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: USHBP1 (Q8N6Y0)\n\nProtein 2: NEFL (P07196)\n Function: Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal interm\n Location: Cell projection, axon\n\nEvidence: Available evidence does not support a physical interaction between USHBP1 and NEFL.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 504, "gene_symbol_1": "USHBP1", "gene_symbol_2": "NEFL", "detection_method": null}} +{"question_id": "PPIL1-0265", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: PLK4 (O00444)\n Function: Serine/threonine-protein kinase that plays a central role in centriole duplication. Able to trigger procentriole formation on the surface of the parental centriole cylinder, leading to the recruitment\n Location: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole\n\nProtein 2: BTRC (A0A0S2Z507)\n\nEvidence: Available evidence does not support a physical interaction between PLK4 and BTRC.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 192, "gene_symbol_1": "PLK4", "gene_symbol_2": "BTRC", "detection_method": null}} +{"question_id": "PPIL1-0266", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: PLP1 (P60201)\n Function: This is the major myelin protein from the central nervous system. It plays an important role in the formation or maintenance of the multilamellar structure of myelin\n Location: Cell membrane\n\nProtein 2: PTPRN (Q16849)\n Function: Plays a role in vesicle-mediated secretory processes (PubMed:24843546). Required for normal accumulation of secretory vesicles in hippocampus, pituitary and pancreatic islets (By similarity). Required\n Location: Membrane\n\nEvidence: Available evidence does not support a physical interaction between PLP1 and PTPRN.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 489, "gene_symbol_1": "PLP1", "gene_symbol_2": "PTPRN", "detection_method": null}} +{"question_id": "PPIL1-0267", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: CEP76 (Q8TAP6)\n Function: Centrosomal protein involved in regulation of centriole duplication. Required to limit centriole duplication to once per cell cycle by preventing centriole reduplication\n Location: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome\n\nProtein 2: CAPN3 (P20807)\n Function: Calcium-regulated non-lysosomal thiol-protease. Proteolytically cleaves CTBP1 at 'His-409'. Mediates, with UTP25, the proteasome-independent degradation of p53/TP53 (PubMed:23357851, PubMed:27657329)\n Location: Cytoplasm\n\nEvidence: Available evidence does not support a physical interaction between CEP76 and CAPN3.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 213, "gene_symbol_1": "CEP76", "gene_symbol_2": "CAPN3", "detection_method": null}} +{"question_id": "PPIL1-0268", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: ACTN4 (A0A0S2Z3G1)\n\nProtein 2: MYOZ2 (Q9NPC6)\n Function: Myozenins may serve as intracellular binding proteins involved in linking Z line proteins such as alpha-actinin, gamma-filamin, TCAP/telethonin, LDB3/ZASP and localizing calcineurin signaling to the s\n Location: Cytoplasm, myofibril, sarcomere, Z line\n\nEvidence: Available evidence does not support a physical interaction between ACTN4 and MYOZ2.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 152, "gene_symbol_1": "ACTN4", "gene_symbol_2": "MYOZ2", "detection_method": null}} +{"question_id": "PPIL1-0269", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: UBQLN1 (Q9UMX0)\n Function: Plays an important role in the regulation of different protein degradation mechanisms and pathways including ubiquitin-proteasome system (UPS), autophagy and endoplasmic reticulum-associated protein d\n Location: Cytoplasm\n\nProtein 2: CLCN2 (A0A0S2Z3J3)\n Location: Membrane\n\nEvidence: Available evidence does not support a physical interaction between UBQLN1 and CLCN2.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 236, "gene_symbol_1": "UBQLN1", "gene_symbol_2": "CLCN2", "detection_method": null}} +{"question_id": "PPIL1-0270", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: TFCP2 (Q12800)\n Function: Binds a variety of cellular and viral promoters including fibrinogen, alpha-globin, SV40 and HIV-1 promoters. Activation of the alpha-globin promoter in erythroid cells is via synergistic interaction \n Location: Nucleus\n\nProtein 2: LSM1 (O15116)\n Function: Plays a role in the degradation of histone mRNAs, the only eukaryotic mRNAs that are not polyadenylated (PubMed:18172165). Probably also part of an LSm subunits-containing complex involved in the gene\n Location: Cytoplasm\n\nEvidence: Available evidence does not support a physical interaction between TFCP2 and LSM1.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 370, "gene_symbol_1": "TFCP2", "gene_symbol_2": "LSM1", "detection_method": null}} +{"question_id": "PPIL1-0271", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: FOXP2 (O15409)\n Function: Transcriptional repressor that may play a role in the specification and differentiation of lung epithelium. May also play a role in developing neural, gastrointestinal and cardiovascular tissues. Can \n Location: Nucleus\n\nProtein 2: TBR1 (Q16650)\n Function: Transcriptional repressor involved in multiple aspects of cortical development, including neuronal migration, laminar and areal identity, and axonal projection (PubMed:25232744, PubMed:30250039). As t\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between FOXP2 and TBR1.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 756, "gene_symbol_1": "FOXP2", "gene_symbol_2": "TBR1", "detection_method": null}} +{"question_id": "PPIL1-0272", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: APBA1 (Q02410)\n Function: Putative function in synaptic vesicle exocytosis by binding to Munc18-1, an essential component of the synaptic vesicle exocytotic machinery. May modulate processing of the amyloid-beta precursor prot\n Location: Cytoplasm\n\nProtein 2: RAB6A (P20340)\n Function: The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes (PubMed:25962623). Rabs cycle between an inactiv\n Location: Golgi apparatus membrane\n\nEvidence: Available evidence does not support a physical interaction between APBA1 and RAB6A.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 104, "gene_symbol_1": "APBA1", "gene_symbol_2": "RAB6A", "detection_method": null}} +{"question_id": "PPIL1-0273", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: ITGB7 (P26010)\n Function: Integrin ITGA4/ITGB7 (alpha-4/beta-7) (Peyer patches-specific homing receptor LPAM-1) is an adhesion molecule that mediates lymphocyte migration and homing to gut-associated lymphoid tissue (GALT) (Pr\n Location: Cell membrane\n\nProtein 2: MAPK8IP1 (Q9UQF2)\n Function: The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. Requir\n Location: Cytoplasm\n\nEvidence: Available evidence does not support a physical interaction between ITGB7 and MAPK8IP1.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "gold", "result_id": 18, "gene_symbol_1": "ITGB7", "gene_symbol_2": "MAPK8IP1", "detection_method": null}} +{"question_id": "PPIL1-0274", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: PPP1R12B (O60237)\n Function: Regulates myosin phosphatase activity. Augments Ca(2+) sensitivity of the contractile apparatus\n Location: Cytoplasm, cytoskeleton\n\nProtein 2: PPP1CB (P62140)\n Function: Protein phosphatase that associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Protein phosphatase (PP1) is essential f\n Location: Cytoplasm\n\nEvidence: Available evidence does not support a physical interaction between PPP1R12B and PPP1CB.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 495, "gene_symbol_1": "PPP1R12B", "gene_symbol_2": "PPP1CB", "detection_method": null}} +{"question_id": "PPIL1-0275", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: NDN (A0A0S2Z3Z2)\n\nProtein 2: SSX2IP (Q9Y2D8)\n Function: Belongs to an adhesion system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). May connect the nectin-afadin and E-cadherin-caten\n Location: Cell junction, adherens junction\n\nEvidence: Available evidence does not support a physical interaction between NDN and SSX2IP.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 424, "gene_symbol_1": "NDN", "gene_symbol_2": "SSX2IP", "detection_method": null}} +{"question_id": "PPIL1-0276", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: FAM9B (Q8IZU0)\n Function: May play a role in meiosis\n Location: Nucleus\n\nProtein 2: HNRPLL (A0A0S2Z5U3)\n\nEvidence: Available evidence does not support a physical interaction between FAM9B and HNRPLL.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 333, "gene_symbol_1": "FAM9B", "gene_symbol_2": "HNRPLL", "detection_method": null}} +{"question_id": "PPIL1-0277", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: REL (Q04864)\n Function: Proto-oncogene that may play a role in differentiation and lymphopoiesis. NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological \n Location: Nucleus\n\nProtein 2: PAK1IP1 (Q9NWT1)\n Function: Negatively regulates the PAK1 kinase. PAK1 is a member of the PAK kinase family, which has been shown to play a positive role in the regulation of signaling pathways involving MAPK8 and RELA. PAK1 exi\n Location: Nucleus, nucleolus\n\nEvidence: Available evidence does not support a physical interaction between REL and PAK1IP1.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 494, "gene_symbol_1": "REL", "gene_symbol_2": "PAK1IP1", "detection_method": null}} +{"question_id": "PPIL1-0278", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: PNMA1 (Q8ND90)\n Location: Nucleus, nucleolus\n\nProtein 2: IVD (P26440)\n Function: A mitochondrial matrix enzyme that catalyzes the third step in leucine catabolism, where isovaleryl-CoA (3-methylbutanoyl-CoA) is metabolized to 3-methylbut-2-enoyl-CoA (PubMed:7640268). To a lesser e\n Location: Mitochondrion matrix\n\nEvidence: Available evidence does not support a physical interaction between PNMA1 and IVD.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 377, "gene_symbol_1": "PNMA1", "gene_symbol_2": "IVD", "detection_method": null}} +{"question_id": "PPIL1-0279", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: ZSCAN20 (P17040)\n Function: May be involved in transcriptional regulation\n Location: Nucleus\n\nProtein 2: ZKSCAN4 (Q969J2)\n Function: May be involved in the transcriptional activation of MDM2 and EP300 genes\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between ZSCAN20 and ZKSCAN4.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 635, "gene_symbol_1": "ZSCAN20", "gene_symbol_2": "ZKSCAN4", "detection_method": null}} +{"question_id": "PPIL1-0280", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: LRRIQ3 (A6PVS8)\n\nProtein 2: NCK2 (E7ERP6)\n\nEvidence: Available evidence does not support a physical interaction between LRRIQ3 and NCK2.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 405, "gene_symbol_1": "LRRIQ3", "gene_symbol_2": "NCK2", "detection_method": null}} +{"question_id": "PPIL1-0281", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: LDB3 (O75112)\n Function: May function as an adapter in striated muscle to couple protein kinase C-mediated signaling via its LIM domains to the cytoskeleton\n Location: Cytoplasm, perinuclear region\n\nProtein 2: TFCP2 (Q12800)\n Function: Binds a variety of cellular and viral promoters including fibrinogen, alpha-globin, SV40 and HIV-1 promoters. Activation of the alpha-globin promoter in erythroid cells is via synergistic interaction \n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between LDB3 and TFCP2.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 369, "gene_symbol_1": "LDB3", "gene_symbol_2": "TFCP2", "detection_method": null}} +{"question_id": "PPIL1-0282", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: HNRNPD (Q14103)\n Function: Binds with high affinity to RNA molecules that contain AU-rich elements (AREs) found within the 3'-UTR of many proto-oncogenes and cytokine mRNAs. Also binds to double- and single-stranded DNA sequenc\n Location: Nucleus\n\nProtein 2: SFN (P31947)\n Function: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways (PubMed:15731107, PubMed:22634725, PubMed:28202711, PubMed:37797010). Binds to a lar\n Location: Cytoplasm\n\nEvidence: Available evidence does not support a physical interaction between HNRNPD and SFN.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "gold", "result_id": 57, "gene_symbol_1": "HNRNPD", "gene_symbol_2": "SFN", "detection_method": null}} +{"question_id": "PPIL1-0283", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: CBY2 (Q8NA61)\n\nProtein 2: METTL17 (Q9H7H0)\n Function: Mitochondrial ribosome (mitoribosome) assembly factor (PubMed:36482135, PubMed:38199006). Binds at the interface of the head and body domains of the mitochondrial small ribosomal subunit (mt-SSU), occ\n Location: Mitochondrion matrix\n\nEvidence: Available evidence does not support a physical interaction between CBY2 and METTL17.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 388, "gene_symbol_1": "CBY2", "gene_symbol_2": "METTL17", "detection_method": null}} +{"question_id": "PPIL1-0284", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: RINT1 (Q6NUQ1)\n Function: Involved in regulation of membrane traffic between the Golgi and the endoplasmic reticulum (ER); the function is proposed to depend on its association in the NRZ complex which is believed to play a ro\n Location: Cytoplasm\n\nProtein 2: TEX9 (A0A0S2Z6S1)\n\nEvidence: Available evidence does not support a physical interaction between RINT1 and TEX9.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 615, "gene_symbol_1": "RINT1", "gene_symbol_2": "TEX9", "detection_method": null}} +{"question_id": "PPIL1-0285", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: CASP2 (A0A0S2Z3I0)\n\nProtein 2: CASP2 (P42575)\n Function: Is a regulator of the cascade of caspases responsible for apoptosis execution (PubMed:11156409, PubMed:15073321, PubMed:8087842). Might function by either activating some proteins required for cell de\n\nEvidence: Available evidence does not support a physical interaction between CASP2 and CASP2.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 218, "gene_symbol_1": "CASP2", "gene_symbol_2": "CASP2", "detection_method": null}} +{"question_id": "PPIL1-0286", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: KRT40 (Q6A162)\n Function: May play a role in late hair differentiation\n\nProtein 2: ZGPAT (A0A0S2Z6H0)\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between KRT40 and ZGPAT.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 644, "gene_symbol_1": "KRT40", "gene_symbol_2": "ZGPAT", "detection_method": null}} +{"question_id": "PPIL1-0287", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: HNRPLL (A0A0S2Z5U3)\n\nProtein 2: DDX39B (Q13838)\n Function: Involved in nuclear export of spliced and unspliced mRNA (PubMed:15833825, PubMed:15998806, PubMed:17190602). Component of the TREX complex which is thought to couple mRNA transcription, processing an\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between HNRPLL and DDX39B.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 339, "gene_symbol_1": "HNRPLL", "gene_symbol_2": "DDX39B", "detection_method": null}} +{"question_id": "PPIL1-0288", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: NEB (P20929)\n Function: This giant muscle protein may be involved in maintaining the structural integrity of sarcomeres and the membrane system associated with the myofibrils. Binds and stabilize F-actin\n Location: Cytoplasm, myofibril, sarcomere\n\nProtein 2: XIRP1 (Q702N8)\n Function: Protects actin filaments from depolymerization (PubMed:15454575). Required for correct cardiac intercalated disk ultrastructure via maintenance of cell-cell adhesion stability, and as a result maintai\n Location: Cell junction, adherens junction\n\nEvidence: Available evidence does not support a physical interaction between NEB and XIRP1.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 773, "gene_symbol_1": "NEB", "gene_symbol_2": "XIRP1", "detection_method": null}} +{"question_id": "PPIL1-0289", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: CDK16 (Q00536)\n Function: Protein kinase that plays a role in vesicle-mediated transport processes and exocytosis. Regulates GH1 release by brain neurons. Phosphorylates NSF, and thereby regulates NSF oligomerization. Required\n Location: Cytoplasm\n\nProtein 2: CCNY (Q8ND76)\n Function: Positive regulatory subunit of the cyclin-dependent kinases CDK14/PFTK1 and CDK16. Acts as a cell-cycle regulator of Wnt signaling pathway during G2/M phase by recruiting CDK14/PFTK1 to the plasma mem\n Location: Cell membrane\n\nEvidence: Available evidence does not support a physical interaction between CDK16 and CCNY.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 754, "gene_symbol_1": "CDK16", "gene_symbol_2": "CCNY", "detection_method": null}} +{"question_id": "PPIL1-0290", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: MVK (F5H8H2)\n Function: Catalyzes the phosphorylation of mevalonate to mevalonate 5-phosphate, a key step in isoprenoid and cholesterol biosynthesis\n Location: Cytoplasm\n\nProtein 2: MVK (Q03426)\n Function: Catalyzes the phosphorylation of mevalonate to mevalonate 5-phosphate, a key step in isoprenoid and cholesterol biosynthesis (PubMed:11278915, PubMed:18302342, PubMed:9325256, PubMed:9392419)\n Location: Cytoplasm\n\nEvidence: Available evidence does not support a physical interaction between MVK and MVK.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 390, "gene_symbol_1": "MVK", "gene_symbol_2": "MVK", "detection_method": null}} +{"question_id": "PPIL1-0291", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: PRKN (O60260)\n Function: Functions within a multiprotein E3 ubiquitin ligase complex, catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins (PubMed:10888878, PubMed:10973942, PubMed:11431533, PubMed\n Location: Cytoplasm, cytosol\n\nProtein 2: FBXO7 (Q9Y3I1)\n Function: Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins and p\n Location: Cytoplasm\n\nEvidence: Available evidence does not support a physical interaction between PRKN and FBXO7.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 106, "gene_symbol_1": "PRKN", "gene_symbol_2": "FBXO7", "detection_method": null}} +{"question_id": "PPIL1-0292", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: ITGB2 (P05107)\n Function: Integrin ITGAL:ITGB2 is a receptor for ICAM1, ICAM2 and ICAM3 (PubMed:1676048, PubMed:23775590, PubMed:38195629). Integrin ITGAL:ITGB2 is also a receptor for the secreted form of ubiquitin-like protei\n Location: Cell membrane\n\nProtein 2: RABGAP1 (Q9Y3P9)\n Function: May act as a GTPase-activating protein of RAB6A. May play a role in microtubule nucleation by centrosome. May participate in a RAB6A-mediated pathway involved in the metaphase-anaphase transition\n Location: Cytoplasm, cytosol\n\nEvidence: Available evidence does not support a physical interaction between ITGB2 and RABGAP1.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "gold", "result_id": 9, "gene_symbol_1": "ITGB2", "gene_symbol_2": "RABGAP1", "detection_method": null}} +{"question_id": "PPIL1-0293", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: AXIN1 (O15169)\n Function: Component of the beta-catenin destruction complex required for regulating CTNNB1 levels through phosphorylation and ubiquitination, and modulating Wnt-signaling (PubMed:12192039, PubMed:27098453, PubM\n Location: Cytoplasm\n\nProtein 2: ENTR1 (Q96C92)\n Function: Endosome-associated protein that plays a role in membrane receptor sorting, cytokinesis and ciliogenesis (PubMed:23108400, PubMed:25278552, PubMed:27767179). Involved in the endosome-to-plasma membran\n Location: Cytoplasm\n\nEvidence: Available evidence does not support a physical interaction between AXIN1 and ENTR1.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 137, "gene_symbol_1": "AXIN1", "gene_symbol_2": "ENTR1", "detection_method": null}} +{"question_id": "PPIL1-0294", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: HNRNPK (P61978)\n Function: One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich seq\n Location: Cytoplasm\n\nProtein 2: RBM14 (Q96PK6)\n Function: Isoform 1 may function as a nuclear receptor coactivator, enhancing transcription through other coactivators such as NCOA6 and CITED1. Isoform 2, functions as a transcriptional repressor, modulating t\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between HNRNPK and RBM14.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 515, "gene_symbol_1": "HNRNPK", "gene_symbol_2": "RBM14", "detection_method": null}} +{"question_id": "PPIL1-0295", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: CRK (P46108)\n Function: Involved in cell branching and adhesion mediated by BCAR1-CRK-RAPGEF1 signaling and activation of RAP1\n Location: Cytoplasm\n\nProtein 2: ELK3 (P41970)\n Function: May be a negative regulator of transcription, but can activate transcription when coexpressed with Ras, Src or Mos. Forms a ternary complex with the serum response factor and the ETS and SRF motifs of\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between CRK and ELK3.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 221, "gene_symbol_1": "CRK", "gene_symbol_2": "ELK3", "detection_method": null}} +{"question_id": "PPIL1-0296", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: NEFL (I6L9F6)\n Function: Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal interm\n Location: Cytoplasm\n\nProtein 2: KLC3 (Q6P597)\n Function: Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. Plays a role during spermiogenesis in the development of the sperm tail midpiece and in the nor\n Location: Cytoplasm, cytoskeleton\n\nEvidence: Available evidence does not support a physical interaction between NEFL and KLC3.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 493, "gene_symbol_1": "NEFL", "gene_symbol_2": "KLC3", "detection_method": null}} +{"question_id": "PPIL1-0297", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: BCL2L1 (Q07817)\n Function: Potent inhibitor of cell death. Inhibits activation of caspases. Appears to regulate cell death by blocking the voltage-dependent anion channel (VDAC) by binding to it and preventing the release of th\n Location: Mitochondrion inner membrane\n\nProtein 2: VAC14 (Q08AM6)\n Function: Scaffold protein component of the PI(3,5)P2 regulatory complex which regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Pentamerizes into a star-shaped\n Location: Endosome membrane\n\nEvidence: Available evidence does not support a physical interaction between BCL2L1 and VAC14.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 201, "gene_symbol_1": "BCL2L1", "gene_symbol_2": "VAC14", "detection_method": null}} +{"question_id": "PPIL1-0298", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: TERT (O14746)\n Function: Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. Active in progenitor and cancer cells. Inactive, or very low activity, in normal somati\n Location: Nucleus, nucleolus\n\nProtein 2: PML (P29590)\n Function: Functions via its association with PML-nuclear bodies (PML-NBs) in a wide range of important cellular processes, including tumor suppression, transcriptional regulation, apoptosis, senescence, DNA dam\n Location: Nucleus\n\nEvidence: Available evidence does not support a physical interaction between TERT and PML.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 93, "gene_symbol_1": "TERT", "gene_symbol_2": "PML", "detection_method": null}} +{"question_id": "PPIL1-0299", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: REL (Q04864)\n Function: Proto-oncogene that may play a role in differentiation and lymphopoiesis. NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological \n Location: Nucleus\n\nProtein 2: S100A1 (P23297)\n Function: Small calcium binding protein that plays important roles in several biological processes such as Ca(2+) homeostasis, chondrocyte biology and cardiomyocyte regulation (PubMed:12804600). In response to \n Location: Cytoplasm\n\nEvidence: Available evidence does not support a physical interaction between REL and S100A1.", "gold_answer": "A", "gold_category": "direct_experimental", "metadata": {"source_db": "intact", "confidence_tier": "silver", "result_id": 552, "gene_symbol_1": "REL", "gene_symbol_2": "S100A1", "detection_method": null}} +{"question_id": "PPIL1-0300", "task": "ppi-l1", "split": "fewshot", "difficulty": "hard", "context_text": "Protein 1: PLA2G10 (O15496)\n Function: Secretory calcium-dependent phospholipase A2 that primarily targets extracellular phospholipids (PubMed:12021277, PubMed:9188469). Hydrolyzes the ester bond of the fatty acyl group attached at sn-2 po\n Location: Secreted\n\nProtein 2: DPYS (Q14117)\n Function: Catalyzes the second step of the reductive pyrimidine degradation, the reversible hydrolytic ring opening of dihydropyrimidines. Can catalyze the ring opening of 5,6-dihydrouracil to N-carbamyl-alanin\n\nEvidence: Systematic testing of PLA2G10 and DPYS in a high-throughput assay did not identify this pair as interacting.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 54536, "gene_symbol_1": "PLA2G10", "gene_symbol_2": "DPYS", "detection_method": null}} +{"question_id": "PPIL1-0301", "task": "ppi-l1", "split": "fewshot", "difficulty": "hard", "context_text": "Protein 1: MRPL41 (Q8IXM3)\n Function: Component of the mitochondrial ribosome large subunit (PubMed:25278503, PubMed:25838379, PubMed:28892042, PubMed:35177605). Also involved in apoptosis and cell cycle (PubMed:16024796, PubMed:16256947)\n Location: Mitochondrion\n\nProtein 2: VPS35 (Q96QK1)\n Function: Acts as a component of the retromer cargo-selective complex (CSC). The CSC is believed to be the core functional component of retromer or respective retromer complex variants acting to prevent missort\n Location: Cytoplasm\n\nEvidence: Systematic testing of MRPL41 and VPS35 in a high-throughput assay did not identify this pair as interacting.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 407232, "gene_symbol_1": "MRPL41", "gene_symbol_2": "VPS35", "detection_method": null}} +{"question_id": "PPIL1-0302", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: FBXO44 (Q9H4M3)\n Function: Substrate-recognition component of two distinct E3 ligase complexes, the SKP1-CUL1-FBXO44 complex and the CUL4B-DDB1-FBXO44 complex (PubMed:18203720, PubMed:25970626). The complex SCF(FBXO44) complex \n Location: Nucleus\n\nProtein 2: SIPA1L2 (Q9P2F8)\n\nEvidence: A large-scale binary interaction screen systematically tested FBXO44 against SIPA1L2. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 487530, "gene_symbol_1": "FBXO44", "gene_symbol_2": "SIPA1L2", "detection_method": null}} +{"question_id": "PPIL1-0303", "task": "ppi-l1", "split": "fewshot", "difficulty": "hard", "context_text": "Protein 1: PCCB (P05166)\n Function: This is one of the 2 subunits of the biotin-dependent propionyl-CoA carboxylase (PCC), a mitochondrial enzyme involved in the catabolism of odd chain fatty acids, branched-chain amino acids isoleucine\n Location: Mitochondrion matrix\n\nProtein 2: TBC1D7 (Q9P0N9)\n Function: Non-catalytic component of the TSC-TBC complex, a multiprotein complex that acts as a negative regulator of the canonical mTORC1 complex, an evolutionarily conserved central nutrient sensor that stimu\n Location: Lysosome membrane\n\nEvidence: Systematic testing of PCCB and TBC1D7 in a high-throughput assay did not identify this pair as interacting.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 131802, "gene_symbol_1": "PCCB", "gene_symbol_2": "TBC1D7", "detection_method": null}} +{"question_id": "PPIL1-0304", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: H3-5 (Q6NXT2)\n Function: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central r\n Location: Nucleus\n\nProtein 2: HM13 (Q8TCT9)\n Function: Catalyzes intramembrane proteolysis of signal peptides that have been removed from precursors of secretory and membrane proteins, resulting in the release of the fragment from the ER membrane into the\n Location: Endoplasmic reticulum membrane\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair H3-5-HM13 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 367570, "gene_symbol_1": "H3-5", "gene_symbol_2": "HM13", "detection_method": null}} +{"question_id": "PPIL1-0305", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: SHMT1 (P34896)\n Function: Pyridoxal phosphate (PLP)-dependent enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate (THF) to glycine and 5,10-methylene THF, serving as a critical component of the folat\n Location: Cytoplasm\n\nProtein 2: TBC1D1 (Q86TI0)\n Function: May act as a GTPase-activating protein for Rab family protein(s). May play a role in the cell cycle and differentiation of various tissues. Involved in the trafficking and translocation of GLUT4-conta\n Location: Nucleus\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair SHMT1-TBC1D1 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 205098, "gene_symbol_1": "SHMT1", "gene_symbol_2": "TBC1D1", "detection_method": null}} +{"question_id": "PPIL1-0306", "task": "ppi-l1", "split": "fewshot", "difficulty": "hard", "context_text": "Protein 1: IFNGR2 (P38484)\n Function: Associates with IFNGR1 to form a receptor for the cytokine interferon gamma (IFNG) (PubMed:7615558, PubMed:7673114, PubMed:8124716). Ligand binding stimulates activation of the JAK/STAT signaling path\n Location: Cell membrane\n\nProtein 2: SMAD5 (Q99717)\n Function: Transcriptional regulator that plays a role in various cellular processes including embryonic development, cell differentiation, angiogenesis and tissue homeostasis (PubMed:12064918, PubMed:16516194).\n Location: Cytoplasm\n\nEvidence: Systematic testing of IFNGR2 and SMAD5 in a high-throughput assay did not identify this pair as interacting.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 210605, "gene_symbol_1": "IFNGR2", "gene_symbol_2": "SMAD5", "detection_method": null}} +{"question_id": "PPIL1-0307", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: RFPL4A (A6NLU0)\n Location: Cytoplasm\n\nProtein 2: OXSR1 (O95747)\n Function: Effector serine/threonine-protein kinase component of the WNK-SPAK/OSR1 kinase cascade, which is involved in various processes, such as ion transport, response to hypertonic stress and blood pressure \n Location: Cytoplasm\n\nEvidence: A large-scale binary interaction screen systematically tested RFPL4A against OXSR1. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 15099, "gene_symbol_1": "RFPL4A", "gene_symbol_2": "OXSR1", "detection_method": null}} +{"question_id": "PPIL1-0308", "task": "ppi-l1", "split": "fewshot", "difficulty": "hard", "context_text": "Protein 1: TMPRSS11D (O60235)\n Function: May play some biological role in the host defense system on the mucous membrane independently of or in cooperation with other substances in airway mucous or bronchial secretions. Plays a role in the p\n Location: Cell membrane\n\nProtein 2: TMEM60 (Q9H2L4)\n Location: Membrane\n\nEvidence: Systematic testing of TMPRSS11D and TMEM60 in a high-throughput assay did not identify this pair as interacting.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 71469, "gene_symbol_1": "TMPRSS11D", "gene_symbol_2": "TMEM60", "detection_method": null}} +{"question_id": "PPIL1-0309", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: ALDH16A1 (Q8IZ83)\n\nProtein 2: FNDC11 (Q9BVV2)\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair ALDH16A1-FNDC11 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 410947, "gene_symbol_1": "ALDH16A1", "gene_symbol_2": "FNDC11", "detection_method": null}} +{"question_id": "PPIL1-0310", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: TBX15 (Q96SF7)\n Function: Probable transcriptional regulator involved in the development of the skeleton of the limb, vertebral column and head. Acts by controlling the number of mesenchymal precursor cells and chondrocytes (B\n Location: Nucleus\n\nProtein 2: APPL1 (Q9UKG1)\n Function: Multifunctional adapter protein that binds to various membrane receptors, nuclear factors and signaling proteins to regulate many processes, such as cell proliferation, immune response, endosomal traf\n Location: Early endosome membrane\n\nEvidence: A large-scale binary interaction screen systematically tested TBX15 against APPL1. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 470456, "gene_symbol_1": "TBX15", "gene_symbol_2": "APPL1", "detection_method": null}} +{"question_id": "PPIL1-0311", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: S100A1 (P23297)\n Function: Small calcium binding protein that plays important roles in several biological processes such as Ca(2+) homeostasis, chondrocyte biology and cardiomyocyte regulation (PubMed:12804600). In response to \n Location: Cytoplasm\n\nProtein 2: CLMP (Q9H6B4)\n Function: May be involved in the cell-cell adhesion. May play a role in adipocyte differentiation and development of obesity. Is required for normal small intestine development\n Location: Cell junction, tight junction\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair S100A1-CLMP did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 184405, "gene_symbol_1": "S100A1", "gene_symbol_2": "CLMP", "detection_method": null}} +{"question_id": "PPIL1-0312", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: SFTPA2 (Q8IWL1)\n Function: In presence of calcium ions, it binds to surfactant phospholipids and contributes to lower the surface tension at the air-liquid interface in the alveoli of the mammalian lung and is essential for nor\n Location: Secreted\n\nProtein 2: SNX21 (Q969T3)\n Function: Binds to membranes enriched in phosphatidylinositol 3-phosphate (PtdIns(P3)) and phosphatidylinositol 4,5-bisphosphate. May be involved in several stages of intracellular trafficking\n Location: Cytoplasmic vesicle membrane\n\nEvidence: A large-scale binary interaction screen systematically tested SFTPA2 against SNX21. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 405572, "gene_symbol_1": "SFTPA2", "gene_symbol_2": "SNX21", "detection_method": null}} +{"question_id": "PPIL1-0313", "task": "ppi-l1", "split": "fewshot", "difficulty": "hard", "context_text": "Protein 1: LRRTM2 (O43300)\n Function: Involved in the development and maintenance of excitatory synapses in the vertebrate nervous system. Regulates surface expression of AMPA receptors and instructs the development of functional glutamat\n Location: Cell membrane\n\nProtein 2: BEST1 (O76090)\n Function: Ligand-gated anion channel that allows the movement of anions across cell membranes when activated by calcium (Ca2+) (PubMed:11904445, PubMed:12907679, PubMed:18179881, PubMed:18400985, PubMed:1985323\n Location: Cell membrane\n\nEvidence: Systematic testing of LRRTM2 and BEST1 in a high-throughput assay did not identify this pair as interacting.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 58493, "gene_symbol_1": "LRRTM2", "gene_symbol_2": "BEST1", "detection_method": null}} +{"question_id": "PPIL1-0314", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: AGPS (O00116)\n Function: Catalyzes the exchange of the acyl chain in acyl-dihydroxyacetonephosphate (acyl-DHAP) for a long chain fatty alcohol, yielding the first ether linked intermediate, i.e. alkyl-dihydroxyacetonephosphat\n Location: Peroxisome membrane\n\nProtein 2: ZFAND2B (Q8WV99)\n Function: Plays a role in protein homeostasis by regulating both the translocation and the ubiquitin-mediated proteasomal degradation of nascent proteins at the endoplasmic reticulum. It is involved in the regu\n Location: Endoplasmic reticulum membrane\n\nEvidence: A large-scale binary interaction screen systematically tested AGPS against ZFAND2B. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 25324, "gene_symbol_1": "AGPS", "gene_symbol_2": "ZFAND2B", "detection_method": null}} +{"question_id": "PPIL1-0315", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: FATE1 (Q969F0)\n Function: Involved in the regulation of endoplasmic reticulum (ER)-mitochondria coupling. Negatively regulates the ER-mitochondria distance and Ca(2+) transfer from ER to mitochondria possibly implicating it in\n Location: Mitochondrion\n\nProtein 2: RAB5A (P20339)\n Function: The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form a\n Location: Cell membrane\n\nEvidence: A large-scale binary interaction screen systematically tested FATE1 against RAB5A. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 177757, "gene_symbol_1": "FATE1", "gene_symbol_2": "RAB5A", "detection_method": null}} +{"question_id": "PPIL1-0316", "task": "ppi-l1", "split": "fewshot", "difficulty": "hard", "context_text": "Protein 1: ZNF443 (Q9Y2A4)\n Function: May be involved in transcriptional regulation\n Location: Nucleus\n\nProtein 2: AP3M1 (Q9Y2T2)\n Function: Part of the AP-3 complex, an adaptor-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding\n Location: Golgi apparatus\n\nEvidence: Systematic testing of ZNF443 and AP3M1 in a high-throughput assay did not identify this pair as interacting.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 500260, "gene_symbol_1": "ZNF443", "gene_symbol_2": "AP3M1", "detection_method": null}} +{"question_id": "PPIL1-0317", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: CDKL5 (O76039)\n Function: Mediates phosphorylation of MECP2 (PubMed:15917271, PubMed:16935860). May regulate ciliogenesis (PubMed:29420175)\n Location: Nucleus\n\nProtein 2: ZNF696 (Q9H7X3)\n Function: May be involved in transcriptional regulation\n Location: Nucleus\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair CDKL5-ZNF696 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 97332, "gene_symbol_1": "CDKL5", "gene_symbol_2": "ZNF696", "detection_method": null}} +{"question_id": "PPIL1-0318", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: LPXN (O60711)\n Function: Transcriptional coactivator for androgen receptor (AR) and serum response factor (SRF). Contributes to the regulation of cell adhesion, spreading and cell migration and acts as a negative regulator in\n Location: Cytoplasm\n\nProtein 2: SUOX (P51687)\n Function: Catalyzes the oxidation of sulfite to sulfate, the terminal reaction in the oxidative degradation of sulfur-containing amino acids\n Location: Mitochondrion intermembrane space\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair LPXN-SUOX did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 77400, "gene_symbol_1": "LPXN", "gene_symbol_2": "SUOX", "detection_method": null}} +{"question_id": "PPIL1-0319", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: TMEM35A (Q53FP2)\n Function: Molecular chaperone which mediates the proper assembly and functional expression of the nicotinic acetylcholine receptors (nAChRs) throughout the brain (PubMed:26875622, PubMed:27789755, PubMed:284457\n Location: Peroxisome membrane\n\nProtein 2: PHAF1 (Q9BSU1)\n Function: Plays a regulatory role in autophagic activity. In complex with BCAS3, associates with the autophagosome formation site during both non-selective and selective autophagy\n Location: Cytoplasm\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair TMEM35A-PHAF1 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 339644, "gene_symbol_1": "TMEM35A", "gene_symbol_2": "PHAF1", "detection_method": null}} +{"question_id": "PPIL1-0320", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: AKR1B1 (P15121)\n Function: Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. Displays enzymatic activity towards endogenous metabolites such as aromatic \n Location: Cytoplasm\n\nProtein 2: ZNF566 (Q969W8)\n Function: May be involved in transcriptional regulation\n Location: Nucleus\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair AKR1B1-ZNF566 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 164790, "gene_symbol_1": "AKR1B1", "gene_symbol_2": "ZNF566", "detection_method": null}} +{"question_id": "PPIL1-0321", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: EML1 (O00423)\n Function: Modulates the assembly and organization of the microtubule cytoskeleton, and probably plays a role in regulating the orientation of the mitotic spindle and the orientation of the plane of cell divisio\n Location: Cytoplasm\n\nProtein 2: SAA2 (P0DJI9)\n Function: Major acute phase reactant\n Location: Secreted\n\nEvidence: A large-scale binary interaction screen systematically tested EML1 against SAA2. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 30107, "gene_symbol_1": "EML1", "gene_symbol_2": "SAA2", "detection_method": null}} +{"question_id": "PPIL1-0322", "task": "ppi-l1", "split": "fewshot", "difficulty": "hard", "context_text": "Protein 1: OCLN (Q16625)\n Function: May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions\n Location: Cell membrane\n\nProtein 2: LRRC36 (Q1X8D7)\n\nEvidence: Systematic testing of OCLN and LRRC36 in a high-throughput assay did not identify this pair as interacting.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 325198, "gene_symbol_1": "OCLN", "gene_symbol_2": "LRRC36", "detection_method": null}} +{"question_id": "PPIL1-0323", "task": "ppi-l1", "split": "fewshot", "difficulty": "hard", "context_text": "Protein 1: SLC13A2 (Q13183)\n Function: Low-affinity sodium-dicarboxylate cotransporter, that mediates the entry of citric acid cycle intermediates, such as succinate, citrate, fumarate and alpha-ketoglutarate (2-oxoglutarate) into the smal\n Location: Apical cell membrane\n\nProtein 2: TMEM17 (Q86X19)\n Function: Transmembrane component of the tectonic-like complex, a complex localized at the transition zone of primary cilia and acting as a barrier that prevents diffusion of transmembrane proteins between the \n Location: Cell projection, cilium membrane\n\nEvidence: Systematic testing of SLC13A2 and TMEM17 in a high-throughput assay did not identify this pair as interacting.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 293458, "gene_symbol_1": "SLC13A2", "gene_symbol_2": "TMEM17", "detection_method": null}} +{"question_id": "PPIL1-0324", "task": "ppi-l1", "split": "fewshot", "difficulty": "hard", "context_text": "Protein 1: CYSTM1 (Q9H1C7)\n Location: Membrane\n\nProtein 2: CLEC4E (Q9ULY5)\n Function: Calcium-dependent lectin that acts as a pattern recognition receptor (PRR) of the innate immune system: recognizes damage-associated molecular patterns (DAMPs) of abnormal self and pathogen-associated\n Location: Cell membrane\n\nEvidence: Systematic testing of CYSTM1 and CLEC4E in a high-throughput assay did not identify this pair as interacting.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 485466, "gene_symbol_1": "CYSTM1", "gene_symbol_2": "CLEC4E", "detection_method": null}} +{"question_id": "PPIL1-0325", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: CCDC6 (Q16204)\n Location: Cytoplasm\n\nProtein 2: BBLN (Q9BUW7)\n Function: Essential for intermediate filament organization in intestinal cells, interacts with intermediate filament and regulates intestinal lumen morphology\n Location: Cell junction\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair CCDC6-BBLN did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 322131, "gene_symbol_1": "CCDC6", "gene_symbol_2": "BBLN", "detection_method": null}} +{"question_id": "PPIL1-0326", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: VWC2 (Q2TAL6)\n Function: BMP antagonist which may play a role in neural development. Promotes cell adhesion (By similarity)\n Location: Secreted, extracellular space, extracellular matrix, basement membrane\n\nProtein 2: TSEN54 (Q7Z6J9)\n Function: Non-catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5' and 3' splice site\n Location: Nucleus\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair VWC2-TSEN54 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 330647, "gene_symbol_1": "VWC2", "gene_symbol_2": "TSEN54", "detection_method": null}} +{"question_id": "PPIL1-0327", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: FUBP3 (Q96I24)\n Function: May interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression\n Location: Nucleus\n\nProtein 2: CIDEB (Q9UHD4)\n Function: Lipid transferase specifically expressed in hepatocytes, which promotes unilocular lipid droplet formation by mediating lipid droplet fusion (PubMed:35939579). Lipid droplet fusion promotes their enla\n Location: Lipid droplet\n\nEvidence: A large-scale binary interaction screen systematically tested FUBP3 against CIDEB. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 460919, "gene_symbol_1": "FUBP3", "gene_symbol_2": "CIDEB", "detection_method": null}} +{"question_id": "PPIL1-0328", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: HIGD1C (A8MV81)\n Location: Membrane\n\nProtein 2: RAB27A (P51159)\n Function: The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form a\n Location: Membrane\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair HIGD1C-RAB27A did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 18423, "gene_symbol_1": "HIGD1C", "gene_symbol_2": "RAB27A", "detection_method": null}} +{"question_id": "PPIL1-0329", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: ZNF574 (Q6ZN55)\n Function: Part of a ribosome quality control that mediates surveillance of ribosome assembly and promotes degradation of ribosomes with assembly defects\n Location: Cytoplasm\n\nProtein 2: TERF2IP (Q9NYB0)\n Function: Acts both as a regulator of telomere function and as a transcription regulator. Involved in the regulation of telomere length and protection as a component of the shelterin complex (telosome). In cont\n Location: Nucleus\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair ZNF574-TERF2IP did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 379852, "gene_symbol_1": "ZNF574", "gene_symbol_2": "TERF2IP", "detection_method": null}} +{"question_id": "PPIL1-0330", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: ZNF774 (Q6NX45)\n Function: May be involved in transcriptional regulation\n Location: Nucleus\n\nProtein 2: CFAP43 (Q8NDM7)\n Function: Flagellar protein involved in sperm flagellum axoneme organization and function (By similarity). Involved in the regulation of the beating frequency of motile cilia on the epithelial cells of the resp\n Location: Cell projection, cilium, flagellum\n\nEvidence: A large-scale binary interaction screen systematically tested ZNF774 against CFAP43. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 367103, "gene_symbol_1": "ZNF774", "gene_symbol_2": "CFAP43", "detection_method": null}} +{"question_id": "PPIL1-0331", "task": "ppi-l1", "split": "fewshot", "difficulty": "hard", "context_text": "Protein 1: CHAF1A (Q13111)\n Function: Acts as a component of the histone chaperone complex chromatin assembly factor 1 (CAF-1), which assembles histone octamers onto DNA during replication and repair. CAF-1 performs the first step of the \n Location: Nucleus\n\nProtein 2: STX1A (Q16623)\n Function: Plays an essential role in hormone and neurotransmitter calcium-dependent exocytosis and endocytosis (PubMed:26635000). Part of the SNARE (Soluble NSF Attachment Receptor) complex composed of SNAP25, \n Location: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane\n\nEvidence: Systematic testing of CHAF1A and STX1A in a high-throughput assay did not identify this pair as interacting.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 291901, "gene_symbol_1": "CHAF1A", "gene_symbol_2": "STX1A", "detection_method": null}} +{"question_id": "PPIL1-0332", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: CLEC12A (Q5QGZ9)\n Function: Myeloid inhibitory C-type lectin receptor that acts as a negative regulator of myeloid cell activation (PubMed:14739280, PubMed:15238421, PubMed:16239426, PubMed:34234773, PubMed:38367667, PubMed:3838\n Location: Cell membrane\n\nProtein 2: REEP4 (Q9H6H4)\n Function: Microtubule-binding protein required to ensure proper cell division and nuclear envelope reassembly by sequestering the endoplasmic reticulum away from chromosomes during mitosis. Probably acts by cle\n Location: Endoplasmic reticulum membrane\n\nEvidence: A large-scale binary interaction screen systematically tested CLEC12A against REEP4. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 347398, "gene_symbol_1": "CLEC12A", "gene_symbol_2": "REEP4", "detection_method": null}} +{"question_id": "PPIL1-0333", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: CDC37 (Q16543)\n Function: Co-chaperone that binds to numerous kinases and promotes their interaction with the Hsp90 complex, resulting in stabilization and promotion of their activity (PubMed:8666233). Inhibits HSP90AA1 ATPase\n Location: Cytoplasm\n\nProtein 2: C10orf67 (Q8IYJ2)\n Location: Mitochondrion\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair CDC37-C10orf67 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 323604, "gene_symbol_1": "CDC37", "gene_symbol_2": "C10orf67", "detection_method": null}} +{"question_id": "PPIL1-0334", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: WDR62 (O43379)\n Function: Required for cerebral cortical development. Plays a role in neuronal proliferation and migration (PubMed:20729831, PubMed:20890278). Plays a role in mother-centriole-dependent centriole duplication; t\n Location: Nucleus\n\nProtein 2: AMELY (Q99218)\n Function: Plays a role in biomineralization. Seems to regulate the formation of crystallites during the secretory stage of tooth enamel development. Thought to play a major role in the structural organization a\n Location: Secreted, extracellular space, extracellular matrix\n\nEvidence: A large-scale binary interaction screen systematically tested WDR62 against AMELY. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 60115, "gene_symbol_1": "WDR62", "gene_symbol_2": "AMELY", "detection_method": null}} +{"question_id": "PPIL1-0335", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: TMEM70 (Q9BUB7)\n Function: Scaffold protein that participates in the c-ring assembly of mitochondrial ATP synthase (F(1)F(0) ATP synthase or complex V) by facilitating the membrane insertion and oligomer formation of the subuni\n Location: Mitochondrion inner membrane\n\nProtein 2: IKZF4 (Q9H2S9)\n Function: DNA-binding protein that binds to the 5'GGGAATRCC-3' Ikaros-binding sequence. Transcriptional repressor. Interacts with SPI1 and MITF to repress transcription of the CTSK and ACP5 promoters via recrui\n Location: Nucleus\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair TMEM70-IKZF4 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 477924, "gene_symbol_1": "TMEM70", "gene_symbol_2": "IKZF4", "detection_method": null}} +{"question_id": "PPIL1-0336", "task": "ppi-l1", "split": "fewshot", "difficulty": "hard", "context_text": "Protein 1: MYH7B (A7E2Y1)\n Function: Involved in muscle contraction\n Location: Membrane\n\nProtein 2: TUBA4A (P68366)\n Function: Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the additio\n Location: Cytoplasm, cytoskeleton\n\nEvidence: Systematic testing of MYH7B and TUBA4A in a high-throughput assay did not identify this pair as interacting.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 16512, "gene_symbol_1": "MYH7B", "gene_symbol_2": "TUBA4A", "detection_method": null}} +{"question_id": "PPIL1-0337", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: CLCA4 (Q14CN2)\n Function: May be involved in mediating calcium-activated chloride conductance\n Location: Cell membrane\n\nProtein 2: PTPN14 (Q15678)\n Function: Protein tyrosine phosphatase which may play a role in the regulation of lymphangiogenesis, cell-cell adhesion, cell-matrix adhesion, cell migration, cell growth and also regulates TGF-beta gene expres\n Location: Cytoplasm\n\nEvidence: A large-scale binary interaction screen systematically tested CLCA4 against PTPN14. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 311409, "gene_symbol_1": "CLCA4", "gene_symbol_2": "PTPN14", "detection_method": null}} +{"question_id": "PPIL1-0338", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: IBSP (P21815)\n Function: Binds tightly to hydroxyapatite (PubMed:11459848). Appears to form an integral part of the mineralized matrix (PubMed:1818768). Probably important to cell-matrix interaction (PubMed:1818768). Promotes\n Location: Secreted\n\nProtein 2: SPINK9 (Q5DT21)\n Function: Serine protease inhibitor which specifically inhibits KLK5. May contribute to the regulation of the desquamation process in skin by inhibiting KLK5\n Location: Secreted\n\nEvidence: A large-scale binary interaction screen systematically tested IBSP against SPINK9. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 180957, "gene_symbol_1": "IBSP", "gene_symbol_2": "SPINK9", "detection_method": null}} +{"question_id": "PPIL1-0339", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: TNFRSF6B (O95407)\n Function: Decoy receptor that can neutralize the cytotoxic ligands TNFS14/LIGHT, TNFSF15 and TNFSF6/FASL. Protects against apoptosis\n Location: Secreted\n\nProtein 2: CSF1 (P09603)\n Function: Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and mono\n Location: Cell membrane\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair TNFRSF6B-CSF1 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 108392, "gene_symbol_1": "TNFRSF6B", "gene_symbol_2": "CSF1", "detection_method": null}} +{"question_id": "PPIL1-0340", "task": "ppi-l1", "split": "fewshot", "difficulty": "hard", "context_text": "Protein 1: ZNF17 (P17021)\n Function: May be involved in transcriptional regulation\n Location: Nucleus\n\nProtein 2: IFT81 (Q8WYA0)\n Function: Component of the intraflagellar transport (IFT) complex B: together with IFT74, forms a tubulin-binding module that specifically mediates transport of tubulin within the cilium. Binds tubulin via its \n Location: Cell projection, cilium\n\nEvidence: Systematic testing of ZNF17 and IFT81 in a high-throughput assay did not identify this pair as interacting.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 170134, "gene_symbol_1": "ZNF17", "gene_symbol_2": "IFT81", "detection_method": null}} +{"question_id": "PPIL1-0341", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: LONRF1 (Q17RB8)\n\nProtein 2: ABHD15 (Q6UXT9)\n Function: May regulate adipocyte lipolysis and liver lipid accumulation\n Location: Secreted\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair LONRF1-ABHD15 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 327932, "gene_symbol_1": "LONRF1", "gene_symbol_2": "ABHD15", "detection_method": null}} +{"question_id": "PPIL1-0342", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: ARSI (Q5FYB1)\n Function: Displays arylsulfatase activity at neutral pH, when co-expressed with SUMF1; arylsulfatase activity is measured in the secretion medium of retinal cell line, but no activity is recorded when measured \n Location: Secreted\n\nProtein 2: HECW1 (Q76N89)\n Function: E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent degradation of DVL1. Also targets the mutant SOD1 protein involved in familial amyotrophic lateral sclerosis (FALS). Forms cytot\n Location: Cytoplasm\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair ARSI-HECW1 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 343416, "gene_symbol_1": "ARSI", "gene_symbol_2": "HECW1", "detection_method": null}} +{"question_id": "PPIL1-0343", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: RPL36A (P83881)\n Function: Component of the large ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell\n Location: Cytoplasm\n\nProtein 2: PMVK (Q15126)\n Function: Catalyzes the reversible ATP-dependent phosphorylation of mevalonate 5-phosphate to produce mevalonate diphosphate and ADP, a key step in the mevalonic acid mediated biosynthesis of isopentenyl diphos\n Location: Cytoplasm, cytosol\n\nEvidence: A large-scale binary interaction screen systematically tested RPL36A against PMVK. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 269822, "gene_symbol_1": "RPL36A", "gene_symbol_2": "PMVK", "detection_method": null}} +{"question_id": "PPIL1-0344", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: ASPSCR1 (Q9BZE9)\n Function: Tethering protein that sequesters GLUT4-containing vesicles in the cytoplasm in the absence of insulin. Modulates the amount of GLUT4 that is available at the cell surface (By similarity). Enhances VC\n Location: Endomembrane system\n\nProtein 2: COQ4 (Q9Y3A0)\n Function: Lyase that catalyzes the C1-decarboxylation of 4-hydroxy-3-methoxy-5-(all-trans-decaprenyl)benzoic acid into 2-methoxy-6-(all-trans-decaprenyl)phenol during ubiquinone biosynthesis\n Location: Mitochondrion inner membrane\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair ASPSCR1-COQ4 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 482462, "gene_symbol_1": "ASPSCR1", "gene_symbol_2": "COQ4", "detection_method": null}} +{"question_id": "PPIL1-0345", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: LIMK1 (P53667)\n Function: Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways (PubMed:10436159, P\n Location: Cytoplasm\n\nProtein 2: MYPOP (Q86VE0)\n Function: Transcriptional repressor; DNA-binding protein that specifically recognizes the core sequence 5'-YAAC[GT]G-3'. Dimerization with PFN1 reduces its DNA-binding capacity (By similarity)\n Location: Nucleus\n\nEvidence: A large-scale binary interaction screen systematically tested LIMK1 against MYPOP. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 239909, "gene_symbol_1": "LIMK1", "gene_symbol_2": "MYPOP", "detection_method": null}} +{"question_id": "PPIL1-0346", "task": "ppi-l1", "split": "fewshot", "difficulty": "hard", "context_text": "Protein 1: ALOX15B (O15296)\n Function: Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids (PUFAs) generating a spectrum of bioactive lipid mediators (Prob\n Location: Nucleus\n\nProtein 2: DUSP23 (Q9BVJ7)\n Function: Protein phosphatase that mediates dephosphorylation of proteins phosphorylated on Tyr and Ser/Thr residues. In vitro, it can dephosphorylate p44-ERK1 (MAPK3) but not p54 SAPK-beta (MAPK10) in vitro. A\n Location: Cytoplasm, cytosol\n\nEvidence: Systematic testing of ALOX15B and DUSP23 in a high-throughput assay did not identify this pair as interacting.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 51128, "gene_symbol_1": "ALOX15B", "gene_symbol_2": "DUSP23", "detection_method": null}} +{"question_id": "PPIL1-0347", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: FSTL1 (Q12841)\n Function: Secreted glycoprotein that is involved in various physiological processes, such as angiogenesis, regulation of the immune response, cell proliferation and differentiation (PubMed:22265692, PubMed:2921\n Location: Secreted\n\nProtein 2: PROK2 (Q9HC23)\n Function: May function as an output molecule from the suprachiasmatic nucleus (SCN) that transmits behavioral circadian rhythm. May also function locally within the SCN to synchronize output. Potently contracts\n Location: Secreted\n\nEvidence: A large-scale binary interaction screen systematically tested FSTL1 against PROK2. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 288698, "gene_symbol_1": "FSTL1", "gene_symbol_2": "PROK2", "detection_method": null}} +{"question_id": "PPIL1-0348", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: OTULINL (Q9NUU6)\n Function: Lacks deubiquitinase activity\n Location: Cytoplasm\n\nProtein 2: USP15 (Q9Y4E8)\n Function: Hydrolase that removes conjugated ubiquitin from target proteins and regulates various pathways such as the TGF-beta receptor signaling, NF-kappa-B and RNF41/NRDP1-PRKN pathways (PubMed:16005295, PubM\n Location: Cytoplasm\n\nEvidence: A large-scale binary interaction screen systematically tested OTULINL against USP15. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 494321, "gene_symbol_1": "OTULINL", "gene_symbol_2": "USP15", "detection_method": null}} +{"question_id": "PPIL1-0349", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: TSGA10IP (Q3SY00)\n\nProtein 2: KIAA1328 (Q86T90)\n Function: Competes with SMC1 for binding to SMC3. May affect the availability of SMC3 to engage in the formation of multimeric protein complexes\n\nEvidence: A large-scale binary interaction screen systematically tested TSGA10IP against KIAA1328. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 334349, "gene_symbol_1": "TSGA10IP", "gene_symbol_2": "KIAA1328", "detection_method": null}} +{"question_id": "PPIL1-0350", "task": "ppi-l1", "split": "fewshot", "difficulty": "hard", "context_text": "Protein 1: VARS1 (P26640)\n Function: Catalyzes the attachment of valine to tRNA(Val)\n\nProtein 2: NAT8L (Q8N9F0)\n Function: Catalyzes the synthesis of N-acetylaspartate acid (NAA) from L-aspartate and acetyl-CoA (PubMed:19524112, PubMed:19807691, PubMed:20385109). Promotes dopamine uptake by regulating TNF expression (By s\n Location: Cytoplasm\n\nEvidence: Systematic testing of VARS1 and NAT8L in a high-throughput assay did not identify this pair as interacting.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 190512, "gene_symbol_1": "VARS1", "gene_symbol_2": "NAT8L", "detection_method": null}} +{"question_id": "PPIL1-0351", "task": "ppi-l1", "split": "fewshot", "difficulty": "hard", "context_text": "Protein 1: PGAM2 (P15259)\n Function: Catalyzes the interconversion of 3- and 2-phosphoglycerate with 2,3-bisphosphoglycerate as the primer of the reaction. Can also catalyze the interconversion of (2R)-2,3-bisphosphoglycerate and (2R)-3-\n\nProtein 2: SRP14 (P37108)\n Function: Component of the signal recognition particle (SRP) complex, a ribonucleoprotein complex that mediates the cotranslational targeting of secretory and membrane proteins to the endoplasmic reticulum (ER)\n Location: Cytoplasm\n\nEvidence: Systematic testing of PGAM2 and SRP14 in a high-throughput assay did not identify this pair as interacting.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 165226, "gene_symbol_1": "PGAM2", "gene_symbol_2": "SRP14", "detection_method": null}} +{"question_id": "PPIL1-0352", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: HUS1 (O60921)\n Function: Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair (PubMed:21659603). The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication\n Location: Nucleus\n\nProtein 2: TOMM6 (Q96B49)\n Function: Component of the translocase of the outer membrane of mitochondria (TOM) complex essential for the recognition and translocation of cytosolically synthesized mitochondrial preproteins (PubMed:40080546\n Location: Mitochondrion outer membrane\n\nEvidence: A large-scale binary interaction screen systematically tested HUS1 against TOMM6. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 80792, "gene_symbol_1": "HUS1", "gene_symbol_2": "TOMM6", "detection_method": null}} +{"question_id": "PPIL1-0353", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: MCEE (Q96PE7)\n Function: Methylmalonyl-CoA epimerase involved in propionyl-CoA metabolism\n Location: Mitochondrion\n\nProtein 2: YIPF2 (Q9BWQ6)\n Location: Golgi apparatus, cis-Golgi network membrane\n\nEvidence: A large-scale binary interaction screen systematically tested MCEE against YIPF2. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 468088, "gene_symbol_1": "MCEE", "gene_symbol_2": "YIPF2", "detection_method": null}} +{"question_id": "PPIL1-0354", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: POLR2M (Q6EEV4)\n\nProtein 2: TREML1 (Q86YW5)\n Function: Cell surface receptor that may play a role in the innate and adaptive immune response\n Location: Cell membrane\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair POLR2M-TREML1 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 362369, "gene_symbol_1": "POLR2M", "gene_symbol_2": "TREML1", "detection_method": null}} +{"question_id": "PPIL1-0355", "task": "ppi-l1", "split": "fewshot", "difficulty": "hard", "context_text": "Protein 1: DEFA5 (Q01523)\n Function: Host-defense peptide that maintains sterility in the urogenital system (PubMed:12021776, PubMed:12660734, PubMed:15616305, PubMed:19589339, PubMed:22359618, PubMed:22573326, PubMed:25354318, PubMed:25\n Location: Secreted\n\nProtein 2: IKZF5 (Q9H5V7)\n Function: Transcriptional repressor that binds the core 5'GNNTGTNG-3' DNA consensus sequence (PubMed:10978333, PubMed:31217188). Involved in megakaryocyte differentiation\n Location: Nucleus\n\nEvidence: Systematic testing of DEFA5 and IKZF5 in a high-throughput assay did not identify this pair as interacting.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 274151, "gene_symbol_1": "DEFA5", "gene_symbol_2": "IKZF5", "detection_method": null}} +{"question_id": "PPIL1-0356", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: SERPING1 (P05155)\n Function: Serine protease inhibitor, which acrs as a regulator of the classical complement pathway (PubMed:10946292, PubMed:11527969, PubMed:3458172, PubMed:6416294). Forms a proteolytically inactive stoichiome\n Location: Secreted\n\nProtein 2: EEF1A2 (Q05639)\n Function: Translation elongation factor that catalyzes the GTP-dependent binding of aminoacyl-tRNA (aa-tRNA) to the A-site of ribosomes during the elongation phase of protein synthesis. Base pairing between the\n Location: Endoplasmic reticulum membrane\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair SERPING1-EEF1A2 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 131331, "gene_symbol_1": "SERPING1", "gene_symbol_2": "EEF1A2", "detection_method": null}} +{"question_id": "PPIL1-0357", "task": "ppi-l1", "split": "fewshot", "difficulty": "hard", "context_text": "Protein 1: CUTC (Q9NTM9)\n Function: May play a role in copper homeostasis. Can bind one Cu(1+) per subunit\n Location: Cytoplasm\n\nProtein 2: NLE1 (Q9NVX2)\n Function: Plays a role in regulating Notch activity. Plays a role in regulating the expression of CDKN1A and several members of the Wnt pathway, probably via its effects on Notch activity. Required during embry\n Location: Nucleus, nucleolus\n\nEvidence: Systematic testing of CUTC and NLE1 in a high-throughput assay did not identify this pair as interacting.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 493959, "gene_symbol_1": "CUTC", "gene_symbol_2": "NLE1", "detection_method": null}} +{"question_id": "PPIL1-0358", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: PRKACB (P22694)\n Function: Mediates cAMP-dependent signaling triggered by receptor binding to GPCRs (PubMed:12420224, PubMed:21423175, PubMed:31112131). PKA activation regulates diverse cellular processes such as cell prolifera\n Location: Cytoplasm\n\nProtein 2: COMP (P49747)\n Function: Plays a role in the structural integrity of cartilage via its interaction with other extracellular matrix proteins such as the collagens and fibronectin. Can mediate the interaction of chondrocytes wi\n Location: Secreted, extracellular space, extracellular matrix\n\nEvidence: A large-scale binary interaction screen systematically tested PRKACB against COMP. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 182947, "gene_symbol_1": "PRKACB", "gene_symbol_2": "COMP", "detection_method": null}} +{"question_id": "PPIL1-0359", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: LRPAP1 (P30533)\n Function: Molecular chaperone for LDL receptor-related proteins that may regulate their ligand binding activity along the secretory pathway\n Location: Rough endoplasmic reticulum lumen\n\nProtein 2: FEM1C (Q96JP0)\n Function: Substrate-recognition component of a Cul2-RING (CRL2) E3 ubiquitin-protein ligase complex of the DesCEND (destruction via C-end degrons) pathway, which recognizes a C-degron located at the extreme C t\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair LRPAP1-FEM1C did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 199079, "gene_symbol_1": "LRPAP1", "gene_symbol_2": "FEM1C", "detection_method": null}} +{"question_id": "PPIL1-0360", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: FANCC (Q00597)\n Function: DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. May be implicated in interstrand DNA cross-link repair and in the maintenance of normal chromosome \n Location: Nucleus\n\nProtein 2: PTCD3 (Q96EY7)\n Function: Mitochondrial RNA-binding protein that has a role in mitochondrial translation\n Location: Mitochondrion\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair FANCC-PTCD3 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 272975, "gene_symbol_1": "FANCC", "gene_symbol_2": "PTCD3", "detection_method": null}} +{"question_id": "PPIL1-0361", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: PXN (P49023)\n Function: Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Recruits other proteins such as TRIM15 to focal adhesion\n Location: Cytoplasm, cytoskeleton\n\nProtein 2: RPAP1 (Q9BWH6)\n Function: Forms an interface between the RNA polymerase II enzyme and chaperone/scaffolding protein, suggesting that it is required to connect RNA polymerase II to regulators of protein complex formation. Requi\n Location: Nucleus\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair PXN-RPAP1 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 225123, "gene_symbol_1": "PXN", "gene_symbol_2": "RPAP1", "detection_method": null}} +{"question_id": "PPIL1-0362", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: TBC1D3K (A0A087X1G2)\n Function: Acts as a GTPase activating protein for RAB5. Does not act on RAB4 or RAB11 (By similarity)\n Location: Cell membrane\n\nProtein 2: HSD17B12 (Q53GQ0)\n Function: Catalyzes the second of the four reactions of the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process, allows the addition of two carbons to the chain of long- \n Location: Endoplasmic reticulum membrane\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair TBC1D3K-HSD17B12 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 1779, "gene_symbol_1": "TBC1D3K", "gene_symbol_2": "HSD17B12", "detection_method": null}} +{"question_id": "PPIL1-0363", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: HSF2BP (O75031)\n Function: Meiotic recombination factor component of recombination bridges involved in meiotic double-strand break repair. Modulates the localization of recombinases DMC1:RAD51 to meiotic double-strand break (DS\n Location: Cytoplasm\n\nProtein 2: BLOC1S1 (P78537)\n Function: Component of the BLOC-1 complex, a complex that is required for normal biogenesis of lysosome-related organelles (LRO), such as platelet dense granules and melanosomes (PubMed:17182842). In concert wi\n Location: Mitochondrion intermembrane space\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair HSF2BP-BLOC1S1 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 81708, "gene_symbol_1": "HSF2BP", "gene_symbol_2": "BLOC1S1", "detection_method": null}} +{"question_id": "PPIL1-0364", "task": "ppi-l1", "split": "val", "difficulty": "hard", "context_text": "Protein 1: LYPD1 (Q8N2G4)\n Function: Believed to act as a modulator of nicotinic acetylcholine receptors (nAChRs) activity. In vitro increases receptor desensitization and decreases affinity for ACh of alpha-4:beta-2-containing nAChRs. M\n Location: Cell membrane\n\nProtein 2: ACOX2 (Q99424)\n Function: Oxidizes the CoA esters of the bile acid intermediates di- and tri-hydroxycholestanoic acids (PubMed:27884763). Capable of oxidizing short as well as long chain 2-methyl branched fatty acids (By simil\n Location: Peroxisome\n\nEvidence: Systematic testing of LYPD1 and ACOX2 in a high-throughput assay did not identify this pair as interacting.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 414754, "gene_symbol_1": "LYPD1", "gene_symbol_2": "ACOX2", "detection_method": null}} +{"question_id": "PPIL1-0365", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: CTPS1 (P17812)\n Function: CTP synthase involved in the de novo synthesis of CTP, a precursor of DNA, RNA and phospholipids (PubMed:16179339, PubMed:17189248, PubMed:17463002, PubMed:24870241, PubMed:28459447, PubMed:34583994).\n Location: Cytoplasm, cytosol\n\nProtein 2: LRRC73 (Q5JTD7)\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair CTPS1-LRRC73 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 173060, "gene_symbol_1": "CTPS1", "gene_symbol_2": "LRRC73", "detection_method": null}} +{"question_id": "PPIL1-0366", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: MUC1 (P15941)\n Function: The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack\n Location: Apical cell membrane\n\nProtein 2: PAIP1 (Q9H074)\n Function: Acts as a coactivator in the regulation of translation initiation of poly(A)-containing mRNAs. Its stimulatory activity on translation is mediated via its action on PABPC1. Competes with PAIP2 for bin\n Location: Cytoplasm\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair MUC1-PAIP1 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 167723, "gene_symbol_1": "MUC1", "gene_symbol_2": "PAIP1", "detection_method": null}} +{"question_id": "PPIL1-0367", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: DENND2C (Q68D51)\n Function: Guanine nucleotide exchange factor (GEF) which may activate RAB9A and RAB9B. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form\n\nProtein 2: ACOT11 (Q8WXI4)\n Function: Has an acyl-CoA thioesterase activity with a preference for the long chain fatty acyl-CoA thioesters hexadecanoyl-CoA/palmitoyl-CoA and tetradecanoyl-CoA/myristoyl-CoA which are the main substrates in\n Location: Mitochondrion matrix\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair DENND2C-ACOT11 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 359760, "gene_symbol_1": "DENND2C", "gene_symbol_2": "ACOT11", "detection_method": null}} +{"question_id": "PPIL1-0368", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: ZNF8 (P17098)\n Function: Transcriptional repressor. May modulate BMP and TGF-beta signal transduction, through its interaction with SMAD proteins\n Location: Nucleus\n\nProtein 2: SLC50A1 (Q9BRV3)\n Function: Mediates sugar transport across membranes. May stimulate V(D)J recombination by the activation of RAG1\n Location: Golgi apparatus membrane\n\nEvidence: A large-scale binary interaction screen systematically tested ZNF8 against SLC50A1. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 171577, "gene_symbol_1": "ZNF8", "gene_symbol_2": "SLC50A1", "detection_method": null}} +{"question_id": "PPIL1-0369", "task": "ppi-l1", "split": "val", "difficulty": "hard", "context_text": "Protein 1: HTN3 (P15516)\n Function: Histatins are cationic and histidine-rich peptides mainly found in the saliva of higher primates (PubMed:3286634). They are considered to be major precursors of the protective proteinaceous structure \n Location: Secreted\n\nProtein 2: POMGNT2 (Q8NAT1)\n Function: O-linked mannose beta-1,4-N-acetylglucosaminyltransferase that transfers UDP-N-acetyl-D-glucosamine to the 4-position of the mannose to generate N-acetyl-D-glucosamine-beta-1,4-O-D-mannosylprotein. In\n Location: Endoplasmic reticulum membrane\n\nEvidence: Systematic testing of HTN3 and POMGNT2 in a high-throughput assay did not identify this pair as interacting.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 166282, "gene_symbol_1": "HTN3", "gene_symbol_2": "POMGNT2", "detection_method": null}} +{"question_id": "PPIL1-0370", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: MTFR1L (Q9H019)\n Function: Mitochondrial protein required for adaptation of miochondrial dynamics to metabolic changes. Regulates mitochondrial morphology at steady state and mediates AMPK-dependent stress-induced mitochondrial\n Location: Mitochondrion outer membrane\n\nProtein 2: POLE4 (Q9NR33)\n Function: Accessory component of the DNA polymerase epsilon complex (PubMed:10801849). Participates in DNA repair and in chromosomal DNA replication (By similarity)\n Location: Nucleus\n\nEvidence: A large-scale binary interaction screen systematically tested MTFR1L against POLE4. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 484243, "gene_symbol_1": "MTFR1L", "gene_symbol_2": "POLE4", "detection_method": null}} +{"question_id": "PPIL1-0371", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: OSMR (Q99650)\n Function: Associates with IL31RA to form the IL31 receptor. Binds IL31 to activate STAT3 and possibly STAT1 and STAT5. Capable of transducing OSM-specific signaling events\n Location: Membrane\n\nProtein 2: TM6SF1 (Q9BZW5)\n Function: May function as sterol isomerase\n Location: Lysosome membrane\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair OSMR-TM6SF1 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 472293, "gene_symbol_1": "OSMR", "gene_symbol_2": "TM6SF1", "detection_method": null}} +{"question_id": "PPIL1-0372", "task": "ppi-l1", "split": "val", "difficulty": "hard", "context_text": "Protein 1: CCN1 (O00622)\n Function: Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in \n Location: Secreted\n\nProtein 2: FIMP (Q96LL3)\n Function: May play a role in sperm-oocyte fusion during fertilization\n Location: Cell membrane\n\nEvidence: Systematic testing of CCN1 and FIMP in a high-throughput assay did not identify this pair as interacting.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 33125, "gene_symbol_1": "CCN1", "gene_symbol_2": "FIMP", "detection_method": null}} +{"question_id": "PPIL1-0373", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: ALDOA (P04075)\n Function: Catalyzes the reversible conversion of beta-D-fructose 1,6-bisphosphate (FBP) into two triose phosphate and plays a key role in glycolysis and gluconeogenesis (PubMed:14766013). In addition, may also \n Location: Cytoplasm, myofibril, sarcomere, I band\n\nProtein 2: CRYBA4 (P53673)\n Function: Crystallins are the dominant structural components of the vertebrate eye lens\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair ALDOA-CRYBA4 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 127829, "gene_symbol_1": "ALDOA", "gene_symbol_2": "CRYBA4", "detection_method": null}} +{"question_id": "PPIL1-0374", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: BTAF1 (O14981)\n Function: Regulates transcription in association with TATA binding protein (TBP). Removes TBP from the TATA box in an ATP-dependent manner\n Location: Nucleus\n\nProtein 2: FBXO48 (Q5FWF7)\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair BTAF1-FBXO48 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 45595, "gene_symbol_1": "BTAF1", "gene_symbol_2": "FBXO48", "detection_method": null}} +{"question_id": "PPIL1-0375", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: VLDLR (P98155)\n Function: Multifunctional cell surface receptor that binds VLDL and transports it into cells by endocytosis and therefore plays an important role in energy metabolism. Also binds to a wide range of other molecu\n Location: Cell membrane\n\nProtein 2: PLIN5 (Q00G26)\n Function: Lipid droplet-associated protein that maintains the balance between lipogenesis and lipolysis and also regulates fatty acid oxidation in oxidative tissues. Recruits mitochondria to the surface of lipi\n Location: Lipid droplet\n\nEvidence: A large-scale binary interaction screen systematically tested VLDLR against PLIN5. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 271180, "gene_symbol_1": "VLDLR", "gene_symbol_2": "PLIN5", "detection_method": null}} +{"question_id": "PPIL1-0376", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: GDPD5 (Q8WTR4)\n Function: Glycerophosphodiester phosphodiesterase that promotes neurite formation and drives spinal motor neuron differentiation (By similarity). Mediates the cleavage of glycosylphosphatidylinositol (GPI) anch\n Location: Endomembrane system\n\nProtein 2: JPH3 (Q8WXH2)\n Function: Junctophilins contribute to the formation of junctional membrane complexes (JMCs) which link the plasma membrane with the endoplasmic or sarcoplasmic reticulum in excitable cells. Provides a structura\n Location: Cell membrane\n\nEvidence: A large-scale binary interaction screen systematically tested GDPD5 against JPH3. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 438243, "gene_symbol_1": "GDPD5", "gene_symbol_2": "JPH3", "detection_method": null}} +{"question_id": "PPIL1-0377", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: VWC2L (B2RUY7)\n Function: May play a role in neurogenesis. May play a role in bone differentiation and matrix mineralization\n Location: Secreted\n\nProtein 2: C2orf68 (Q2NKX9)\n\nEvidence: A large-scale binary interaction screen systematically tested VWC2L against C2orf68. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 19716, "gene_symbol_1": "VWC2L", "gene_symbol_2": "C2orf68", "detection_method": null}} +{"question_id": "PPIL1-0378", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: CDC27 (P30260)\n Function: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle (PubMed:18485873)\n Location: Nucleus\n\nProtein 2: THOC7 (Q6I9Y2)\n Function: Component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspl\n Location: Cytoplasm\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair CDC27-THOC7 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 198007, "gene_symbol_1": "CDC27", "gene_symbol_2": "THOC7", "detection_method": null}} +{"question_id": "PPIL1-0379", "task": "ppi-l1", "split": "val", "difficulty": "hard", "context_text": "Protein 1: DBX1 (A6NMT0)\n Function: Could have a role in patterning the central nervous system during embryogenesis. Has a key role in regulating the distinct phenotypic features that distinguish two major classes of ventral interneuron\n Location: Nucleus\n\nProtein 2: RNF128 (Q8TEB7)\n Function: E3 ubiquitin-protein ligase that catalyzes 'Lys-27', 'Lys-48'- or 'Lys-63'-linked polyubiquitin chains formation and plays a role in different biological processes such as modulation of immune respons\n Location: Cytoplasm\n\nEvidence: Systematic testing of DBX1 and RNF128 in a high-throughput assay did not identify this pair as interacting.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 15614, "gene_symbol_1": "DBX1", "gene_symbol_2": "RNF128", "detection_method": null}} +{"question_id": "PPIL1-0380", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: IQCF3 (P0C7M6)\n\nProtein 2: ERO1A (Q96HE7)\n Function: Oxidoreductase involved in disulfide bond formation in the endoplasmic reticulum. Efficiently reoxidizes P4HB/PDI, the enzyme catalyzing protein disulfide formation, in order to allow P4HB to sustain \n Location: Endoplasmic reticulum membrane\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair IQCF3-ERO1A did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 147650, "gene_symbol_1": "IQCF3", "gene_symbol_2": "ERO1A", "detection_method": null}} +{"question_id": "PPIL1-0381", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: FSD2 (A1L4K1)\n Location: Nucleus\n\nProtein 2: C1QTNF5 (Q9BXJ0)\n Location: Secreted\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair FSD2-C1QTNF5 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 8627, "gene_symbol_1": "FSD2", "gene_symbol_2": "C1QTNF5", "detection_method": null}} +{"question_id": "PPIL1-0382", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: IFRD2 (Q12894)\n Function: Ribosome-binding protein that acts as an inhibitor of mRNA translation by promoting ribosome inactivation (PubMed:30355441). Associates with the P- and E-sites of the ribosome and inserts a C-terminal\n\nProtein 2: FXYD6 (Q9H0Q3)\n Function: Associates with and regulates the activity of the sodium/potassium-transporting ATPase (NKA) which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma me\n Location: Cell membrane\n\nEvidence: A large-scale binary interaction screen systematically tested IFRD2 against FXYD6. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 289358, "gene_symbol_1": "IFRD2", "gene_symbol_2": "FXYD6", "detection_method": null}} +{"question_id": "PPIL1-0383", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: PDGFRB (P09619)\n Function: Tyrosine-protein kinase that acts as a cell-surface receptor for homodimeric PDGFB and PDGFD and for heterodimers formed by PDGFA and PDGFB, and plays an essential role in the regulation of embryonic \n Location: Cell membrane\n\nProtein 2: CCDC88C (Q9P219)\n Function: Required for activation of guanine nucleotide-binding proteins (G-proteins) during non-canonical Wnt signaling (PubMed:26126266). Binds to ligand-activated Wnt receptor FZD7, displacing DVL1 from the \n Location: Cytoplasm\n\nEvidence: A large-scale binary interaction screen systematically tested PDGFRB against CCDC88C. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 145390, "gene_symbol_1": "PDGFRB", "gene_symbol_2": "CCDC88C", "detection_method": null}} +{"question_id": "PPIL1-0384", "task": "ppi-l1", "split": "val", "difficulty": "hard", "context_text": "Protein 1: TTR (P02766)\n Function: Thyroid hormone-binding protein. Probably transports thyroxine from the bloodstream to the brain\n Location: Secreted\n\nProtein 2: IFNA4 (P05014)\n Function: Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase\n Location: Secreted\n\nEvidence: Systematic testing of TTR and IFNA4 in a high-throughput assay did not identify this pair as interacting.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 126139, "gene_symbol_1": "TTR", "gene_symbol_2": "IFNA4", "detection_method": null}} +{"question_id": "PPIL1-0385", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: FBL (P22087)\n Function: S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins (PubMed:24352239, PubMed:30540930, PubMed:32017898). Involved in pre-rRNA processing by cat\n Location: Nucleus, nucleolus\n\nProtein 2: MYSM1 (Q5VVJ2)\n Function: Metalloprotease with deubiquitinase activity that plays important regulator roles in hematopoietic stem cell function, blood cell production and immune response (PubMed:24062447, PubMed:26220525, PubM\n Location: Nucleus\n\nEvidence: A large-scale binary interaction screen systematically tested FBL against MYSM1. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 181734, "gene_symbol_1": "FBL", "gene_symbol_2": "MYSM1", "detection_method": null}} +{"question_id": "PPIL1-0386", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: RAB27B (O00194)\n Function: The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form a\n Location: Membrane\n\nProtein 2: UBD (O15205)\n Function: Ubiquitin-like protein modifier which can be covalently attached to target proteins and subsequently leads to their degradation by the 26S proteasome, in a NUB1-dependent manner (PubMed:15831455, PubM\n Location: Nucleus\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair RAB27B-UBD did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 27017, "gene_symbol_1": "RAB27B", "gene_symbol_2": "UBD", "detection_method": null}} +{"question_id": "PPIL1-0387", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: KIF21B (O75037)\n Function: Plus-end directed microtubule-dependent motor protein which displays processive activity. Is involved in regulation of microtubule dynamics, synapse function and neuronal morphology, including dendrit\n Location: Cytoplasm, cytoskeleton\n\nProtein 2: SACM1L (Q9NTJ5)\n Function: Phosphoinositide phosphatase which catalyzes the hydrolysis of phosphatidylinositol 4-phosphate (PtdIns(4)P) (PubMed:24209621, PubMed:27044890, PubMed:29461204, PubMed:30659099). Can also catalyze the\n Location: Endoplasmic reticulum membrane\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair KIF21B-SACM1L did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 81848, "gene_symbol_1": "KIF21B", "gene_symbol_2": "SACM1L", "detection_method": null}} +{"question_id": "PPIL1-0388", "task": "ppi-l1", "split": "val", "difficulty": "hard", "context_text": "Protein 1: FTH1 (P02794)\n Function: Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity (PubMed:9003196). Iron is taken up in the ferrous form and deposited as ferric hyd\n Location: Cytoplasm\n\nProtein 2: PLAC9 (Q5JTB6)\n Location: Secreted\n\nEvidence: Systematic testing of FTH1 and PLAC9 in a high-throughput assay did not identify this pair as interacting.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 126556, "gene_symbol_1": "FTH1", "gene_symbol_2": "PLAC9", "detection_method": null}} +{"question_id": "PPIL1-0389", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: FHIT (P49789)\n Function: Possesses dinucleoside triphosphate hydrolase activity (PubMed:12574506, PubMed:15182206, PubMed:8794732, PubMed:9323207, PubMed:9543008, PubMed:9576908). Cleaves P(1)-P(3)-bis(5'-adenosyl) triphospha\n Location: Cytoplasm\n\nProtein 2: KRTAP19-3 (Q7Z4W3)\n Function: In the hair cortex, hair keratin intermediate filaments are embedded in an interfilamentous matrix, consisting of hair keratin-associated proteins (KRTAP), which are essential for the formation of a r\n\nEvidence: A large-scale binary interaction screen systematically tested FHIT against KRTAP19-3. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 229191, "gene_symbol_1": "FHIT", "gene_symbol_2": "KRTAP19-3", "detection_method": null}} +{"question_id": "PPIL1-0390", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: MARCHF6 (O60337)\n Function: Endoplasmic reticulum membrane-associated E3 ubiquitin ligase that plays a critical role in mitigating endoplasmic reticulum stress, the regulation of cholesterol and lipid homeostasis, and ferroptosi\n Location: Endoplasmic reticulum membrane\n\nProtein 2: DIDO1 (Q9BTC0)\n Function: Putative transcription factor, weakly pro-apoptotic when overexpressed (By similarity). Tumor suppressor. Required for early embryonic stem cell development\n Location: Cytoplasm\n\nEvidence: A large-scale binary interaction screen systematically tested MARCHF6 against DIDO1. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 73393, "gene_symbol_1": "MARCHF6", "gene_symbol_2": "DIDO1", "detection_method": null}} +{"question_id": "PPIL1-0391", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: LDB3 (O75112)\n Function: May function as an adapter in striated muscle to couple protein kinase C-mediated signaling via its LIM domains to the cytoskeleton\n Location: Cytoplasm, perinuclear region\n\nProtein 2: PIAS2 (O75928)\n Function: Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptio\n Location: Nucleus speckle\n\nEvidence: A large-scale binary interaction screen systematically tested LDB3 against PIAS2. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 82670, "gene_symbol_1": "LDB3", "gene_symbol_2": "PIAS2", "detection_method": null}} +{"question_id": "PPIL1-0392", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: ARPP19 (P56211)\n Function: Protein phosphatase inhibitor that specifically inhibits protein phosphatase 2A (PP2A) during mitosis (PubMed:38123684). Inhibition of PP2A is enhanced when ARPP19 is phosphorylated (PubMed:38123684).\n Location: Cytoplasm\n\nProtein 2: MYNN (Q9NPC7)\n Location: Nucleus\n\nEvidence: A large-scale binary interaction screen systematically tested ARPP19 against MYNN. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 246678, "gene_symbol_1": "ARPP19", "gene_symbol_2": "MYNN", "detection_method": null}} +{"question_id": "PPIL1-0393", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: CDKN2C (P42773)\n Function: Interacts strongly with CDK6, weakly with CDK4. Inhibits cell growth and proliferation with a correlated dependence on endogenous retinoblastoma protein RB\n\nProtein 2: MLIP (Q5VWP3)\n Function: Required for myoblast differentiation into myotubes, possibly acting as a transcriptional regulator of the myogenic program (By similarity). Required for cardiac adaptation to stress through integrate\n Location: Nucleus\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair CDKN2C-MLIP did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 216389, "gene_symbol_1": "CDKN2C", "gene_symbol_2": "MLIP", "detection_method": null}} +{"question_id": "PPIL1-0394", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: EVX2 (Q03828)\n Location: Nucleus\n\nProtein 2: ZCWPW2 (Q504Y3)\n Function: Histone methylation reader which binds to non-methylated (H3K4me0), monomethylated (H3K4me1), dimethylated (H3K4me2) and trimethylated (H3K4me3) 'Lys-4' on histone H3 (PubMed:26933034). The order of b\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair EVX2-ZCWPW2 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 278233, "gene_symbol_1": "EVX2", "gene_symbol_2": "ZCWPW2", "detection_method": null}} +{"question_id": "PPIL1-0395", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: CETN3 (O15182)\n Function: Plays a fundamental role in microtubule-organizing center structure and function\n Location: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome\n\nProtein 2: HSPA12A (O43301)\n Function: Adapter protein for SORL1, but not SORT1. Delays SORL1 internalization and affects SORL1 subcellular localization\n Location: Cytoplasm\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair CETN3-HSPA12A did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 48883, "gene_symbol_1": "CETN3", "gene_symbol_2": "HSPA12A", "detection_method": null}} +{"question_id": "PPIL1-0396", "task": "ppi-l1", "split": "val", "difficulty": "hard", "context_text": "Protein 1: SMARCB1 (Q12824)\n Function: Core component of the BAF (hSWI/SNF) complex. This ATP-dependent chromatin-remodeling complex plays important roles in cell proliferation and differentiation, in cellular antiviral activities and inhi\n Location: Nucleus\n\nProtein 2: SMAD9 (O15198)\n Function: Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD9 is a receptor-regulated SMAD (R-SMAD)\n Location: Cytoplasm\n\nEvidence: Systematic testing of SMARCB1 and SMAD9 in a high-throughput assay did not identify this pair as interacting.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 49133, "gene_symbol_1": "SMARCB1", "gene_symbol_2": "SMAD9", "detection_method": null}} +{"question_id": "PPIL1-0397", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: SEPTIN7 (Q16181)\n Function: Filament-forming cytoskeletal GTPase. Required for normal organization of the actin cytoskeleton. Required for normal progress through mitosis. Involved in cytokinesis. Required for normal association\n Location: Cytoplasm\n\nProtein 2: SUSD4 (Q5VX71)\n Function: Acts as a complement inhibitor by disrupting the formation of the classical C3 convertase. Isoform 3 inhibits the classical complement pathway, while membrane-bound isoform 1 inhibits deposition of C3\n Location: Membrane\n\nEvidence: A large-scale binary interaction screen systematically tested SEPTIN7 against SUSD4. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 321958, "gene_symbol_1": "SEPTIN7", "gene_symbol_2": "SUSD4", "detection_method": null}} +{"question_id": "PPIL1-0398", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: NXF2 (Q9GZY0)\n Function: Involved in the export of mRNA from the nucleus to the cytoplasm\n Location: Nucleus, nucleoplasm\n\nProtein 2: BCL2L10 (Q9HD36)\n Function: Promotes cell survival by suppressing apoptosis induced by BAX but not BAK (PubMed:11278245, PubMed:11689480). Increases binding of AHCYL1/IRBIT to ITPR1 (PubMed:27995898). Reduces ITPR1-mediated calc\n Location: Mitochondrion\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair NXF2-BCL2L10 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 484125, "gene_symbol_1": "NXF2", "gene_symbol_2": "BCL2L10", "detection_method": null}} +{"question_id": "PPIL1-0399", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: INS-IGF2 (F8WCM5)\n\nProtein 2: PQBP1 (O60828)\n Function: Intrinsically disordered protein that acts as a scaffold, and which is involved in different processes, such as pre-mRNA splicing, transcription regulation, innate immunity and neuron development (Pub\n Location: Nucleus\n\nEvidence: A large-scale binary interaction screen systematically tested INS-IGF2 against PQBP1. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 23292, "gene_symbol_1": "INS-IGF2", "gene_symbol_2": "PQBP1", "detection_method": null}} +{"question_id": "PPIL1-0400", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: HAPSTR1 (Q14CZ0)\n Function: Acts as a central player within a network of stress response pathways promoting cellular adaptability. The E3 ligase HUWE1 assists HAPSTR1 in controlling stress signaling and in turn, HUWE1 feeds back\n Location: Nucleus\n\nProtein 2: TMED8 (Q6PL24)\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair HAPSTR1-TMED8 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 311761, "gene_symbol_1": "HAPSTR1", "gene_symbol_2": "TMED8", "detection_method": null}} +{"question_id": "PPIL1-0401", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: NCF2 (P19878)\n Function: Subunit of the phagocyte NADPH oxidase complex that mediates the transfer of electrons from cytosolic NADPH to O2 to produce the superoxide anion (O2(-)) (PubMed:12207919, PubMed:38355798). In the act\n Location: Cytoplasm\n\nProtein 2: KHDC3L (Q587J8)\n Function: Component of the subcortical maternal complex (SCMC), a multiprotein complex that plays a key role in early embryonic development (By similarity). The SCMC complex is a structural constituent of cytop\n Location: Cytoplasm\n\nEvidence: A large-scale binary interaction screen systematically tested NCF2 against KHDC3L. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 176459, "gene_symbol_1": "NCF2", "gene_symbol_2": "KHDC3L", "detection_method": null}} +{"question_id": "PPIL1-0402", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: NBR1 (Q14596)\n Function: Ubiquitin-binding autophagy adapter that participates in different processes including host defense or intracellular homeostasis (PubMed:24692539, PubMed:33577621). Possesses a double function during \n Location: Cytoplasm\n\nProtein 2: P3H3 (Q8IVL6)\n Function: Part of a complex composed of PLOD1, P3H3 and P3H4 that catalyzes hydroxylation of lysine residues in collagen alpha chains and is required for normal assembly and cross-linkling of collagen fibrils. \n Location: Endoplasmic reticulum\n\nEvidence: A large-scale binary interaction screen systematically tested NBR1 against P3H3. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 308251, "gene_symbol_1": "NBR1", "gene_symbol_2": "P3H3", "detection_method": null}} +{"question_id": "PPIL1-0403", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: SEZ6L2 (Q6UXD5)\n Function: May contribute to specialized endoplasmic reticulum functions in neurons\n Location: Cell membrane\n\nProtein 2: DECR2 (Q9NUI1)\n Function: Auxiliary enzyme of beta-oxidation. Participates in the degradation of unsaturated fatty enoyl-CoA esters having double bonds in both even- and odd-numbered positions in peroxisome. Catalyzes the NADP\n Location: Peroxisome\n\nEvidence: A large-scale binary interaction screen systematically tested SEZ6L2 against DECR2. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 377103, "gene_symbol_1": "SEZ6L2", "gene_symbol_2": "DECR2", "detection_method": null}} +{"question_id": "PPIL1-0404", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: LAYN (Q6UX15)\n Function: Receptor for hyaluronate\n Location: Membrane\n\nProtein 2: GOLGA6L2 (Q8N9W4)\n\nEvidence: A large-scale binary interaction screen systematically tested LAYN against GOLGA6L2. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 376439, "gene_symbol_1": "LAYN", "gene_symbol_2": "GOLGA6L2", "detection_method": null}} +{"question_id": "PPIL1-0405", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: AGTRAP (Q6RW13)\n Function: Appears to be a negative regulator of type-1 angiotensin II receptor-mediated signaling by regulating receptor internalization as well as mechanism of receptor desensitization such as phosphorylation.\n Location: Endoplasmic reticulum membrane\n\nProtein 2: FADS6 (A0A087WWN9)\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair AGTRAP-FADS6 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 950, "gene_symbol_1": "AGTRAP", "gene_symbol_2": "FADS6", "detection_method": null}} +{"question_id": "PPIL1-0406", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: PCDH17 (O14917)\n Function: Potential calcium-dependent cell-adhesion protein\n Location: Cell membrane\n\nProtein 2: GPKOW (Q92917)\n Function: RNA-binding protein involved in pre-mRNA splicing. As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs (Probable)\n Location: Nucleus\n\nEvidence: A large-scale binary interaction screen systematically tested PCDH17 against GPKOW. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 43602, "gene_symbol_1": "PCDH17", "gene_symbol_2": "GPKOW", "detection_method": null}} +{"question_id": "PPIL1-0407", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: PFKP (Q01813)\n Function: Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis\n Location: Cytoplasm\n\nProtein 2: MTERF2 (Q49AM1)\n Function: Binds mitochondrial DNA and plays a role in the regulation of transcription of mitochondrial mRNA and rRNA species\n Location: Mitochondrion\n\nEvidence: A large-scale binary interaction screen systematically tested PFKP against MTERF2. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 274891, "gene_symbol_1": "PFKP", "gene_symbol_2": "MTERF2", "detection_method": null}} +{"question_id": "PPIL1-0408", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: PRPF3 (O43395)\n Function: Plays a role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex)\n Location: Nucleus\n\nProtein 2: CKM (P06732)\n Function: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with larg\n Location: Cytoplasm\n\nEvidence: A large-scale binary interaction screen systematically tested PRPF3 against CKM. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 60265, "gene_symbol_1": "PRPF3", "gene_symbol_2": "CKM", "detection_method": null}} +{"question_id": "PPIL1-0409", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: PLCXD3 (Q63HM9)\n Location: Cytoplasm\n\nProtein 2: RCL1 (Q9Y2P8)\n Function: As part of the small subunit (SSU) processome, it plays a role in 40S-ribosomal-subunit biogenesis in the early pre-rRNA processing steps at sites A0, A1 and A2 that are required for proper maturation\n Location: Nucleus, nucleolus\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair PLCXD3-RCL1 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 358357, "gene_symbol_1": "PLCXD3", "gene_symbol_2": "RCL1", "detection_method": null}} +{"question_id": "PPIL1-0410", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: C1QA (P02745)\n Function: Core component of the complement C1 complex, a multiprotein complex that initiates the classical pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pat\n Location: Secreted\n\nProtein 2: CD7 (P09564)\n Function: Transmembrane glycoprotein expressed by T-cells and natural killer (NK) cells and their precursors (PubMed:7506726). Plays a costimulatory role in T-cell activation upon binding to its ligand K12/SECT\n Location: Membrane\n\nEvidence: A large-scale binary interaction screen systematically tested C1QA against CD7. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 125765, "gene_symbol_1": "C1QA", "gene_symbol_2": "CD7", "detection_method": null}} +{"question_id": "PPIL1-0411", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: CCDC148 (Q8NFR7)\n\nProtein 2: RNF141 (Q8WVD5)\n Function: May be involved in spermatogenesis\n Location: Membrane\n\nEvidence: A large-scale binary interaction screen systematically tested CCDC148 against RNF141. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 429454, "gene_symbol_1": "CCDC148", "gene_symbol_2": "RNF141", "detection_method": null}} +{"question_id": "PPIL1-0412", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: INTS5 (Q6P9B9)\n Function: Component of the integrator complex, a multiprotein complex that terminates RNA polymerase II (Pol II) transcription in the promoter-proximal region of genes (PubMed:33243860, PubMed:38570683). The in\n Location: Nucleus\n\nProtein 2: TBC1D17 (Q9HA65)\n Function: Probable RAB GTPase-activating protein that inhibits RAB8A/B function. Reduces Rab8 recruitment to tubules emanating from the endocytic recycling compartment (ERC) and inhibits Rab8-mediated endocytic\n Location: Cytoplasmic vesicle, autophagosome\n\nEvidence: A large-scale binary interaction screen systematically tested INTS5 against TBC1D17. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 370454, "gene_symbol_1": "INTS5", "gene_symbol_2": "TBC1D17", "detection_method": null}} +{"question_id": "PPIL1-0413", "task": "ppi-l1", "split": "val", "difficulty": "hard", "context_text": "Protein 1: TSPO2 (Q5TGU0)\n Function: Cholesterol-binding protein involved in the redistribution of cholesterol from lipid droplets to the endoplasmic reticulum (PubMed:19729679). Required to meet cholesterol demands during erythropoietic\n Location: Endoplasmic reticulum membrane\n\nProtein 2: TMEM107 (Q6UX40)\n Function: Plays a role in cilia formation and embryonic patterning. Requires for normal Sonic hedgehog (Shh) signaling in the neural tube and acts in combination with GLI2 and GLI3 to pattern ventral and interm\n Location: Membrane\n\nEvidence: Systematic testing of TSPO2 and TMEM107 in a high-throughput assay did not identify this pair as interacting.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 354005, "gene_symbol_1": "TSPO2", "gene_symbol_2": "TMEM107", "detection_method": null}} +{"question_id": "PPIL1-0414", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: CCER1 (Q8TC90)\n Function: Regulator of histone epigenetic modifications and chromatin compaction into the sperm head, required for histone-to-protamine (HTP) transition. HTP is a key event in which somatic histones are first r\n Location: Nucleus\n\nProtein 2: CHRD (Q9H2X0)\n Function: Dorsalizing factor. Key developmental protein that dorsalizes early vertebrate embryonic tissues by binding to ventralizing TGF-beta family bone morphogenetic proteins (BMPs) and sequestering them in \n Location: Secreted\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair CCER1-CHRD did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 434535, "gene_symbol_1": "CCER1", "gene_symbol_2": "CHRD", "detection_method": null}} +{"question_id": "PPIL1-0415", "task": "ppi-l1", "split": "val", "difficulty": "hard", "context_text": "Protein 1: TIMM10 (P62072)\n Function: Mitochondrial intermembrane chaperone that participates in the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. May also be required for the transfer of\n Location: Mitochondrion inner membrane\n\nProtein 2: DENND1C (Q8IV53)\n Function: Guanine nucleotide exchange factor (GEF) which may activate RAB8A, RAB13 and RAB35. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form\n Location: Cytoplasm, cytosol\n\nEvidence: Systematic testing of TIMM10 and DENND1C in a high-throughput assay did not identify this pair as interacting.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 258401, "gene_symbol_1": "TIMM10", "gene_symbol_2": "DENND1C", "detection_method": null}} +{"question_id": "PPIL1-0416", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: LCE2C (Q5TA81)\n Function: Precursors of the cornified envelope of the stratum\n\nProtein 2: GJB4 (Q9NTQ9)\n Function: Structural component of gap junctions (By similarity). Gap junctions are dodecameric channels that connect the cytoplasm of adjoining cells. They are formed by the docking of two hexameric hemichannel\n Location: Cell membrane\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair LCE2C-GJB4 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 353239, "gene_symbol_1": "LCE2C", "gene_symbol_2": "GJB4", "detection_method": null}} +{"question_id": "PPIL1-0417", "task": "ppi-l1", "split": "val", "difficulty": "hard", "context_text": "Protein 1: KSR2 (Q6VAB6)\n Function: Location-regulated scaffold connecting MEK to RAF. Has very low protein kinase activity and can phosphorylate MAP2K1 at several Ser and Thr residues with very low efficiency (in vitro). Acts as MAP2K1\n Location: Cytoplasm\n\nProtein 2: SEMA3G (Q9NS98)\n Function: Has chemorepulsive activities for sympathetic axons. Ligand of NRP2 (By similarity)\n Location: Secreted\n\nEvidence: Systematic testing of KSR2 and SEMA3G in a high-throughput assay did not identify this pair as interacting.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 378251, "gene_symbol_1": "KSR2", "gene_symbol_2": "SEMA3G", "detection_method": null}} +{"question_id": "PPIL1-0418", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: MAGEA4 (P43358)\n Function: Regulates cell proliferation through the inhibition of cell cycle arrest at the G1 phase (PubMed:22842486). Also negatively regulates p53-mediated apoptosis (PubMed:22842486)\n\nProtein 2: NUP54 (Q7Z3B4)\n Function: Component of the nuclear pore complex, a complex required for the trafficking across the nuclear membrane\n Location: Nucleus, nuclear pore complex\n\nEvidence: A large-scale binary interaction screen systematically tested MAGEA4 against NUP54. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 217866, "gene_symbol_1": "MAGEA4", "gene_symbol_2": "NUP54", "detection_method": null}} +{"question_id": "PPIL1-0419", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: KRT31 (Q15323)\n\nProtein 2: PPP1R8 (Q12972)\n Function: Inhibitor subunit of the major nuclear protein phosphatase-1 (PP-1). It has RNA-binding activity but does not cleave RNA and may target PP-1 to RNA-associated substrates. May also be involved in pre-m\n Location: Nucleus\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair KRT31-PPP1R8 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 290307, "gene_symbol_1": "KRT31", "gene_symbol_2": "PPP1R8", "detection_method": null}} +{"question_id": "PPIL1-0420", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: CDKL3 (Q8IVW4)\n Location: Cytoplasm\n\nProtein 2: NOB1 (Q9ULX3)\n Function: May play a role in mRNA degradation (Probable). Endonuclease required for processing of 20S pre-rRNA precursor and biogenesis of 40S ribosomal subunits (By similarity)\n Location: Nucleus\n\nEvidence: A large-scale binary interaction screen systematically tested CDKL3 against NOB1. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 404775, "gene_symbol_1": "CDKL3", "gene_symbol_2": "NOB1", "detection_method": null}} +{"question_id": "PPIL1-0421", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: SHQ1 (Q6PI26)\n Function: Required for the quantitative accumulation of H/ACA ribonucleoproteins (RNPs), including telomerase, probably through the stabilization of DKC1, from the time of its synthesis until its association wi\n Location: Cytoplasm, cytosol\n\nProtein 2: STRAP (Q9Y3F4)\n Function: The SMN complex catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, and thereby plays an important role in the splicing of cellular pre-mRNAs. \n Location: Cytoplasm\n\nEvidence: A large-scale binary interaction screen systematically tested SHQ1 against STRAP. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 371676, "gene_symbol_1": "SHQ1", "gene_symbol_2": "STRAP", "detection_method": null}} +{"question_id": "PPIL1-0422", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: APRT (P07741)\n Function: Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis\n Location: Cytoplasm\n\nProtein 2: BCL2 (P10415)\n Function: Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells (PubMed:1508712, PubMed:8183370). Regulates cell death by controlling the mitochondria\n Location: Mitochondrion outer membrane\n\nEvidence: A large-scale binary interaction screen systematically tested APRT against BCL2. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 138265, "gene_symbol_1": "APRT", "gene_symbol_2": "BCL2", "detection_method": null}} +{"question_id": "PPIL1-0423", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: GAGE2A (Q6NT46)\n\nProtein 2: CT45A3 (Q8NHU0)\n Location: Nucleus\n\nEvidence: Systematic testing of GAGE2A and CT45A3 in a high-throughput assay did not identify this pair as interacting.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 366177, "gene_symbol_1": "GAGE2A", "gene_symbol_2": "CT45A3", "detection_method": null}} +{"question_id": "PPIL1-0424", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: GYS1 (P13807)\n Function: Glycogen synthase participates in the glycogen biosynthetic process along with glycogenin and glycogen branching enzyme. Extends the primer composed of a few glucose units formed by glycogenin by addi\n\nProtein 2: ZNF473 (Q8WTR7)\n Function: Involved in histone 3'-end pre-mRNA processing by associating with U7 snRNP and interacting with SLBP/pre-mRNA complex. Increases histone 3'-end pre-mRNA processing but has no effect on U7 snRNP level\n Location: Nucleus\n\nEvidence: A large-scale binary interaction screen systematically tested GYS1 against ZNF473. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 161455, "gene_symbol_1": "GYS1", "gene_symbol_2": "ZNF473", "detection_method": null}} +{"question_id": "PPIL1-0425", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: SNRPB2 (P08579)\n Function: Involved in pre-mRNA splicing as component of the spliceosome (PubMed:11991638, PubMed:28076346, PubMed:28502770, PubMed:28781166, PubMed:32494006). Associated with sn-RNP U2, where it contributes to \n Location: Nucleus\n\nProtein 2: C19orf18 (Q8NEA5)\n Location: Membrane\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair SNRPB2-C19orf18 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 141367, "gene_symbol_1": "SNRPB2", "gene_symbol_2": "C19orf18", "detection_method": null}} +{"question_id": "PPIL1-0426", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: GPR161 (Q8N6U8)\n Function: Key negative regulator of Shh signaling, which promotes the processing of GLI3 into GLI3R during neural tube development. Recruited by TULP3 and the IFT-A complex to primary cilia and acts as a regula\n Location: Cell projection, cilium membrane\n\nProtein 2: C7orf25 (Q9BPX7)\n\nEvidence: A large-scale binary interaction screen systematically tested GPR161 against C7orf25. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 420736, "gene_symbol_1": "GPR161", "gene_symbol_2": "C7orf25", "detection_method": null}} +{"question_id": "PPIL1-0427", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: GLRA1 (P23415)\n Function: Subunit of heteromeric glycine-gated chloride channels (PubMed:14551753, PubMed:23994010, PubMed:25730860, PubMed:37821459). Plays an important role in the down-regulation of neuronal excitability (Pu\n Location: Postsynaptic cell membrane\n\nProtein 2: IL17A (Q16552)\n Function: Effector cytokine of innate and adaptive immune system involved in antimicrobial host defense and maintenance of tissue integrity (PubMed:24120361). Signals via IL17RA-IL17RC heterodimeric receptor co\n Location: Secreted\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair GLRA1-IL17A did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 184812, "gene_symbol_1": "GLRA1", "gene_symbol_2": "IL17A", "detection_method": null}} +{"question_id": "PPIL1-0428", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: TAF1B (Q53T94)\n Function: Component of RNA polymerase I core factor complex that acts as a GTF2B/TFIIB-like factor and plays a key role in multiple steps during transcription initiation such as pre-initiation complex (PIC) ass\n Location: Nucleus, nucleolus\n\nProtein 2: CCDC60 (Q8IWA6)\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair TAF1B-CCDC60 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 341203, "gene_symbol_1": "TAF1B", "gene_symbol_2": "CCDC60", "detection_method": null}} +{"question_id": "PPIL1-0429", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: CHURC1 (Q8WUH1)\n Function: Transcriptional activator that mediates FGF signaling during neural development (By similarity). Plays a role in the regulation of cell movement (By similarity)\n\nProtein 2: CSRNP1 (Q96S65)\n Function: Binds to the consensus sequence 5'-AGAGTG-3' and has transcriptional activator activity (By similarity). May have a tumor-suppressor function. May play a role in apoptosis\n Location: Nucleus\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair CHURC1-CSRNP1 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 439233, "gene_symbol_1": "CHURC1", "gene_symbol_2": "CSRNP1", "detection_method": null}} +{"question_id": "PPIL1-0430", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: SFI1 (A8K8P3)\n Function: Plays a role in the dynamic structure of centrosome-associated contractile fibers via its interaction with CETN2\n Location: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole\n\nProtein 2: GTPBP8 (Q8N3Z3)\n\nEvidence: Systematic testing of SFI1 and GTPBP8 in a high-throughput assay did not identify this pair as interacting.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 16991, "gene_symbol_1": "SFI1", "gene_symbol_2": "GTPBP8", "detection_method": null}} +{"question_id": "PPIL1-0431", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: FBXO46 (Q6PJ61)\n Function: Substrate-recognition component of the SCF(FBXO46) protein ligase complex, which mediates the ubiquitination and degradation of target proteins (PubMed:30171069). In absence of stress, the SCF(FBXO46)\n\nProtein 2: MRRF (Q96E11)\n Function: Responsible for the disassembly of ribosomes from messenger RNA at the termination of mitochondrial protein biosynthesis (PubMed:19716793, PubMed:33878294). Acts in collaboration with GFM2 (PubMed:338\n Location: Mitochondrion\n\nEvidence: Systematic testing of FBXO46 and MRRF in a high-throughput assay did not identify this pair as interacting.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 372377, "gene_symbol_1": "FBXO46", "gene_symbol_2": "MRRF", "detection_method": null}} +{"question_id": "PPIL1-0432", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: MYOG (P15173)\n Function: Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation, cell cycle exit and muscle atrophy. Essential for the develo\n Location: Nucleus\n\nProtein 2: KATNAL2 (Q8IYT4)\n Function: Severs microtubules in vitro in an ATP-dependent manner. This activity may promote rapid reorganization of cellular microtubule arrays\n Location: Cytoplasm, cytoskeleton\n\nEvidence: A large-scale binary interaction screen systematically tested MYOG against KATNAL2. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 165165, "gene_symbol_1": "MYOG", "gene_symbol_2": "KATNAL2", "detection_method": null}} +{"question_id": "PPIL1-0433", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: PAK3 (O75914)\n Function: Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, or cell cycle regulation. Plays a role in dendrite spi\n Location: Cytoplasm\n\nProtein 2: GABPB2 (Q8TAK5)\n Function: May function as transcription factor capable of interacting with purine rich repeats (GA repeats)\n Location: Nucleus\n\nEvidence: A large-scale binary interaction screen systematically tested PAK3 against GABPB2. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 95392, "gene_symbol_1": "PAK3", "gene_symbol_2": "GABPB2", "detection_method": null}} +{"question_id": "PPIL1-0434", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: FRS3 (O43559)\n Function: Adapter protein that links FGF and NGF receptors to downstream signaling pathways. Involved in the activation of MAP kinases. Down-regulates ERK2 signaling by interfering with the phosphorylation and \n Location: Membrane\n\nProtein 2: CPT2 (P23786)\n Function: Involved in the intramitochondrial synthesis of acylcarnitines from accumulated acyl-CoA metabolites (PubMed:20538056, PubMed:24780397). Reconverts acylcarnitines back into the respective acyl-CoA est\n Location: Mitochondrion inner membrane\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair FRS3-CPT2 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 62621, "gene_symbol_1": "FRS3", "gene_symbol_2": "CPT2", "detection_method": null}} +{"question_id": "PPIL1-0435", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: RPS27A (P62979)\n Function: Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a pol\n Location: Cytoplasm\n\nProtein 2: CNIH2 (Q6PI25)\n Function: Regulates the trafficking and gating properties of AMPA-selective glutamate receptors (AMPARs). Promotes their targeting to the cell membrane and synapses and modulates their gating properties by regu\n Location: Endoplasmic reticulum membrane\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair RPS27A-CNIH2 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 262451, "gene_symbol_1": "RPS27A", "gene_symbol_2": "CNIH2", "detection_method": null}} +{"question_id": "PPIL1-0436", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: MPST (P25325)\n Function: Transfer of a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity. Detoxifies cyanide and is required for thiosulfate biosynthesis. Acts as an antioxidant. In combinati\n Location: Cytoplasm\n\nProtein 2: TPX2 (Q9ULW0)\n Function: Spindle assembly factor required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule\n Location: Nucleus\n\nEvidence: A large-scale binary interaction screen systematically tested MPST against TPX2. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 188075, "gene_symbol_1": "MPST", "gene_symbol_2": "TPX2", "detection_method": null}} +{"question_id": "PPIL1-0437", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: DYRK3 (O43781)\n Function: Dual-specificity protein kinase that promotes disassembly of several types of membraneless organelles during mitosis, such as stress granules, nuclear speckles and pericentriolar material (PubMed:2997\n Location: Nucleus\n\nProtein 2: TBC1D3 (Q8IZP1)\n Function: Acts as a GTPase activating protein for RAB5. Does not act on RAB4 or RAB11\n Location: Cell membrane\n\nEvidence: A large-scale binary interaction screen systematically tested DYRK3 against TBC1D3. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 68170, "gene_symbol_1": "DYRK3", "gene_symbol_2": "TBC1D3", "detection_method": null}} +{"question_id": "PPIL1-0438", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: PIP4K2B (P78356)\n Function: Participates in the biosynthesis of phosphatidylinositol 4,5-bisphosphate (PubMed:26774281, PubMed:9038203). Preferentially utilizes GTP, rather than ATP, for PI(5)P phosphorylation and its activity r\n Location: Endoplasmic reticulum membrane\n\nProtein 2: CUL5 (Q93034)\n Function: Core component of multiple cullin-5-RING E3 ubiquitin-protein ligase complexes (ECS complexes, also named CRL5 complexes), which mediate the ubiquitination and subsequent proteasomal degradation of ta\n Location: Nucleus\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair PIP4K2B-CUL5 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 266543, "gene_symbol_1": "PIP4K2B", "gene_symbol_2": "CUL5", "detection_method": null}} +{"question_id": "PPIL1-0439", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: NEU1 (Q99519)\n Function: Catalyzes the removal of sialic acid (N-acetylneuraminic acid) moieties from glycoproteins and glycolipids. To be active, it is strictly dependent on its presence in the multienzyme complex. Appears t\n Location: Lysosome membrane\n\nProtein 2: PCDHB3 (Q9Y5E6)\n Function: Potential calcium-dependent cell-adhesion protein. May be involved in the establishment and maintenance of specific neuronal connections in the brain\n Location: Cell membrane\n\nEvidence: A large-scale binary interaction screen systematically tested NEU1 against PCDHB3. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 471443, "gene_symbol_1": "NEU1", "gene_symbol_2": "PCDHB3", "detection_method": null}} +{"question_id": "PPIL1-0440", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: FKBP1B (P68106)\n Function: Has the potential to contribute to the immunosuppressive and toxic effects of FK506 and rapamycin. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidi\n Location: Cytoplasm\n\nProtein 2: POU6F1 (Q14863)\n Function: Transcription factor that binds preferentially to a variant of the octamer motif (5'-ATGATAAT-3')\n Location: Nucleus\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair FKBP1B-POU6F1 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 264907, "gene_symbol_1": "FKBP1B", "gene_symbol_2": "POU6F1", "detection_method": null}} +{"question_id": "PPIL1-0441", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: TEP1 (Q99973)\n Function: Component of the telomerase ribonucleoprotein complex that is essential for the replication of chromosome termini (PubMed:19179534). Also a component of the ribonucleoprotein vaults particle, a multi-\n Location: Nucleus\n\nProtein 2: PTGES2 (Q9H7Z7)\n Function: Isomerase that catalyzes the conversion of PGH2 into the more stable prostaglandin E2 (PGE2) (in vitro) (PubMed:12804604, PubMed:17585783, PubMed:18198127). The biological function and the GSH-depende\n Location: Golgi apparatus membrane\n\nEvidence: A large-scale binary interaction screen systematically tested TEP1 against PTGES2. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 473725, "gene_symbol_1": "TEP1", "gene_symbol_2": "PTGES2", "detection_method": null}} +{"question_id": "PPIL1-0442", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: PRRG1 (O14668)\n Location: Membrane\n\nProtein 2: AKR7A3 (O95154)\n Function: Catalyzes the NADPH-dependent reduction of various carbonyl-containing compounds, including aldehydes, ketones, and toxic products from cellular metabolism or environmental exposure. Can reduce the di\n Location: Cytoplasm\n\nEvidence: A large-scale binary interaction screen systematically tested PRRG1 against AKR7A3. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 38771, "gene_symbol_1": "PRRG1", "gene_symbol_2": "AKR7A3", "detection_method": null}} +{"question_id": "PPIL1-0443", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: BOLA2B (H3BTW0)\n\nProtein 2: RHBDL2 (Q9NX52)\n Function: Involved in regulated intramembrane proteolysis and the subsequent release of functional polypeptides from their membrane anchors. Known substrate: EFNB3\n Location: Cell membrane\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair BOLA2B-RHBDL2 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 24504, "gene_symbol_1": "BOLA2B", "gene_symbol_2": "RHBDL2", "detection_method": null}} +{"question_id": "PPIL1-0444", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: TRIML2 (Q8N7C3)\n\nProtein 2: GNE (Q9Y223)\n Function: Bifunctional enzyme that possesses both UDP-N-acetylglucosamine 2-epimerase and N-acetylmannosamine kinase activities, and serves as the initiator of the biosynthetic pathway leading to the production\n Location: Cytoplasm, cytosol\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair TRIML2-GNE did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 421191, "gene_symbol_1": "TRIML2", "gene_symbol_2": "GNE", "detection_method": null}} +{"question_id": "PPIL1-0445", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: HNRNPK (P61978)\n Function: One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich seq\n Location: Cytoplasm\n\nProtein 2: VSTM1 (Q6UX27)\n Function: Behaves as a cytokine, promoting IL17A secretion by CD4+ T-cells, and differentiation and activation of IL17 producing helper T-cells (TH17)\n Location: Membrane\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair HNRNPK-VSTM1 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 258056, "gene_symbol_1": "HNRNPK", "gene_symbol_2": "VSTM1", "detection_method": null}} +{"question_id": "PPIL1-0446", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: LMX1B (O60663)\n Function: Transcription factor involved in the regulation of podocyte-expressed genes (PubMed:24042019, PubMed:28059119). Essential for the specification of dorsal limb fate at both the zeugopodal and autopodal\n Location: Nucleus\n\nProtein 2: KCNJ11 (Q14654)\n Function: Inward rectifier potassium channel that forms the pore of ATP-sensitive potassium channels (KATP), regulating potassium permeability as a function of cytoplasmic ATP and ADP concentrations in many dif\n Location: Membrane\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair LMX1B-KCNJ11 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 76578, "gene_symbol_1": "LMX1B", "gene_symbol_2": "KCNJ11", "detection_method": null}} +{"question_id": "PPIL1-0447", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: ITPRIPL1 (Q6GPH6)\n Function: Functions as a ligand of CD3E, inhibiting TCR-CD3 complex signaling to regulate T cell activation. Induces stable CD3E-NCK1 binding, thereby preventing the CD3E-ZAP70 interaction and subsequently inhi\n Location: Cell membrane\n\nProtein 2: ARRDC3 (Q96B67)\n Function: Adapter protein that plays a role in regulating cell-surface expression of adrenergic receptors and probably also other G protein-coupled receptors (PubMed:20559325, PubMed:21982743, PubMed:23208550).\n Location: Cytoplasm\n\nEvidence: Systematic testing of ITPRIPL1 and ARRDC3 in a high-throughput assay did not identify this pair as interacting.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 362914, "gene_symbol_1": "ITPRIPL1", "gene_symbol_2": "ARRDC3", "detection_method": null}} +{"question_id": "PPIL1-0448", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: KRTAP10-10 (P60014)\n Function: In the hair cortex, hair keratin intermediate filaments are embedded in an interfilamentous matrix, consisting of hair keratin-associated proteins (KRTAP), which are essential for the formation of a r\n\nProtein 2: DDX43 (Q9NXZ2)\n\nEvidence: A large-scale binary interaction screen systematically tested KRTAP10-10 against DDX43. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 252216, "gene_symbol_1": "KRTAP10-10", "gene_symbol_2": "DDX43", "detection_method": null}} +{"question_id": "PPIL1-0449", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: SOCS6 (O14544)\n Function: SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. May be a substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-b\n\nProtein 2: CETN2 (P41208)\n Function: Plays a fundamental role in microtubule organizing center structure and function. Required for centriole duplication and correct spindle formation. Has a role in regulating cytokinesis and genome stab\n Location: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair SOCS6-CETN2 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 36216, "gene_symbol_1": "SOCS6", "gene_symbol_2": "CETN2", "detection_method": null}} +{"question_id": "PPIL1-0450", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: PIM1 (P11309)\n Function: Proto-oncogene with serine/threonine kinase activity involved in cell survival and cell proliferation and thus providing a selective advantage in tumorigenesis (PubMed:15528381, PubMed:1825810, PubMed\n Location: Cytoplasm\n\nProtein 2: H2BC4 (P62807)\n Function: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central r\n Location: Nucleus\n\nEvidence: Systematic testing of PIM1 and H2BC4 in a high-throughput assay did not identify this pair as interacting.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 156415, "gene_symbol_1": "PIM1", "gene_symbol_2": "H2BC4", "detection_method": null}} +{"question_id": "PPIL1-0451", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: GDPD5 (Q8WTR4)\n Function: Glycerophosphodiester phosphodiesterase that promotes neurite formation and drives spinal motor neuron differentiation (By similarity). Mediates the cleavage of glycosylphosphatidylinositol (GPI) anch\n Location: Endomembrane system\n\nProtein 2: DYRK4 (Q9NR20)\n Function: Possible non-essential role in spermiogenesis\n Location: Cytoplasm\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair GDPD5-DYRK4 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 438268, "gene_symbol_1": "GDPD5", "gene_symbol_2": "DYRK4", "detection_method": null}} +{"question_id": "PPIL1-0452", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: TMEM234 (Q8WY98)\n Location: Membrane\n\nProtein 2: EEF2KMT (Q96G04)\n Function: Catalyzes the trimethylation of eukaryotic elongation factor 2 (EEF2) on 'Lys-525'\n Location: Cytoplasm\n\nEvidence: A large-scale binary interaction screen systematically tested TMEM234 against EEF2KMT. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 443924, "gene_symbol_1": "TMEM234", "gene_symbol_2": "EEF2KMT", "detection_method": null}} +{"question_id": "PPIL1-0453", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: SYN3 (O14994)\n Function: May be involved in the regulation of neurotransmitter release and synaptogenesis\n Location: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane\n\nProtein 2: CCDC158 (Q5M9N0)\n Function: Plays a role in receptor-mediated endocytosis in proximal tubular cells of the kidney\n Location: Cytoplasmic vesicle, clathrin-coated vesicle\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair SYN3-CCDC158 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 45826, "gene_symbol_1": "SYN3", "gene_symbol_2": "CCDC158", "detection_method": null}} +{"question_id": "PPIL1-0454", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: ZFTRAF1 (P0DTL6)\n Location: Cytoplasm\n\nProtein 2: KRTAP7-1 (Q8IUC3)\n Function: In the hair cortex, hair keratin intermediate filaments are embedded in an interfilamentous matrix, consisting of hair keratin-associated proteins (KRTAP), which are essential for the formation of a r\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair ZFTRAF1-KRTAP7-1 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 152164, "gene_symbol_1": "ZFTRAF1", "gene_symbol_2": "KRTAP7-1", "detection_method": null}} +{"question_id": "PPIL1-0455", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: PTPN3 (P26045)\n Function: May act at junctions between the membrane and the cytoskeleton. Possesses tyrosine phosphatase activity\n Location: Cell membrane\n\nProtein 2: APOBEC3B (Q9UH17)\n Function: DNA deaminase (cytidine deaminase) which acts as an inhibitor of retrovirus replication and retrotransposon mobility via deaminase-dependent and -independent mechanisms. After the penetration of retro\n Location: Nucleus\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair PTPN3-APOBEC3B did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 189316, "gene_symbol_1": "PTPN3", "gene_symbol_2": "APOBEC3B", "detection_method": null}} +{"question_id": "PPIL1-0456", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: B4GALT1 (P15291)\n Function: Galactosyltransferase acting in the Golgi stacks. Catalyzes the transfer of galactose (Gal) from UDP-alpha-D-galactose in beta(1->4) linkage to the non-reducing terminal N-acetylglucosamine (GlcNAc) m\n Location: Golgi apparatus, Golgi stack membrane\n\nProtein 2: SWSAP1 (Q6NVH7)\n Function: ATPase which is preferentially stimulated by single-stranded DNA and is involved in homologous recombination repair (HRR). Has a DNA-binding activity which is independent of its ATPase activity\n Location: Nucleus\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair B4GALT1-SWSAP1 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 165544, "gene_symbol_1": "B4GALT1", "gene_symbol_2": "SWSAP1", "detection_method": null}} +{"question_id": "PPIL1-0457", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: ZKSCAN1 (P17029)\n Function: May be involved in transcriptional regulation\n Location: Nucleus\n\nProtein 2: SCGB3A1 (Q96QR1)\n Function: Secreted cytokine-like protein. Inhibits cell growth in vitro\n Location: Secreted\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair ZKSCAN1-SCGB3A1 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 170717, "gene_symbol_1": "ZKSCAN1", "gene_symbol_2": "SCGB3A1", "detection_method": null}} +{"question_id": "PPIL1-0458", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: SF3B4 (Q15427)\n Function: Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs (PubMed:10882114, PubMed:12234937, PubMed:27720643, PubMed:3\n Location: Nucleus\n\nProtein 2: HP1BP3 (Q5SSJ5)\n Function: Component of heterochromatin that maintains heterochromatin integrity during G1/S progression and regulates the duration of G1 phase to critically influence cell proliferative capacity (PubMed:2483041\n Location: Nucleus\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair SF3B4-HP1BP3 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 316559, "gene_symbol_1": "SF3B4", "gene_symbol_2": "HP1BP3", "detection_method": null}} +{"question_id": "PPIL1-0459", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: SELENOV (P59797)\n Function: May be involved in a redox-related process\n\nProtein 2: PDRG1 (Q9NUG6)\n Function: May play a role in chaperone-mediated protein folding\n Location: Cytoplasm\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair SELENOV-PDRG1 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 251388, "gene_symbol_1": "SELENOV", "gene_symbol_2": "PDRG1", "detection_method": null}} +{"question_id": "PPIL1-0460", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: FLNA (P21333)\n Function: Promotes orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. Anchors various transmembrane proteins to the actin cytoskeleton and serves as a scaffold for a wi\n Location: Cytoplasm, cell cortex\n\nProtein 2: GIP (P09681)\n Function: Potent stimulator of insulin secretion and relatively poor inhibitor of gastric acid secretion\n Location: Secreted\n\nEvidence: Systematic testing of FLNA and GIP in a high-throughput assay did not identify this pair as interacting.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 145767, "gene_symbol_1": "FLNA", "gene_symbol_2": "GIP", "detection_method": null}} +{"question_id": "PPIL1-0461", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: AKAP5 (P24588)\n Function: Multivalent scaffold protein that anchors the cAMP-dependent protein kinase/PKA to cytoskeletal and/or organelle-associated proteins, targeting the signal carried by cAMP to specific intracellular eff\n Location: Postsynaptic recycling endosome membrane\n\nProtein 2: TRAK1 (Q9UPV9)\n Function: Involved in the regulation of endosome-to-lysosome trafficking, including endocytic trafficking of EGF-EGFR complexes and GABA-A receptors (PubMed:18675823). Involved in mitochondrial motility. When O\n Location: Cytoplasm\n\nEvidence: A large-scale binary interaction screen systematically tested AKAP5 against TRAK1. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 186821, "gene_symbol_1": "AKAP5", "gene_symbol_2": "TRAK1", "detection_method": null}} +{"question_id": "PPIL1-0462", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: ETHE1 (O95571)\n Function: Sulfur dioxygenase that plays an essential role in hydrogen sulfide catabolism in the mitochondrial matrix. Hydrogen sulfide (H(2)S) is first oxidized by SQRDL, giving rise to cysteine persulfide resi\n Location: Cytoplasm\n\nProtein 2: COMMD9 (Q9P000)\n Function: Scaffold protein in the commander complex that is essential for endosomal recycling of transmembrane cargos; the commander complex is composed of the CCC subcomplex and the retriever subcomplex (PubMe\n Location: Nucleus\n\nEvidence: A large-scale binary interaction screen systematically tested ETHE1 against COMMD9. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 110755, "gene_symbol_1": "ETHE1", "gene_symbol_2": "COMMD9", "detection_method": null}} +{"question_id": "PPIL1-0463", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: METTL9 (Q9H1A3)\n Function: Protein-histidine N-methyltransferase that specifically catalyzes 1-methylhistidine (pros-methylhistidine) methylation of target proteins (PubMed:33563959, PubMed:34562450, PubMed:37015930, PubMed:373\n Location: Endoplasmic reticulum\n\nProtein 2: CXXC1 (Q9P0U4)\n Function: Transcriptional activator that exhibits a unique DNA binding specificity for CpG unmethylated motifs with a preference for CpGG\n Location: Nucleus speckle\n\nEvidence: Systematic testing of METTL9 and CXXC1 in a high-throughput assay did not identify this pair as interacting.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 485405, "gene_symbol_1": "METTL9", "gene_symbol_2": "CXXC1", "detection_method": null}} +{"question_id": "PPIL1-0464", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: FOXP3 (Q9BZS1)\n Function: Transcriptional regulator which is crucial for the development and inhibitory function of regulatory T-cells (Treg) (PubMed:17377532, PubMed:21458306, PubMed:23947341, PubMed:24354325, PubMed:24722479\n Location: Nucleus\n\nProtein 2: ANXA11 (P50995)\n Function: Binds specifically to calcyclin in a calcium-dependent manner (By similarity). Required for midbody formation and completion of the terminal phase of cytokinesis\n Location: Cytoplasm\n\nEvidence: Systematic testing of FOXP3 and ANXA11 in a high-throughput assay did not identify this pair as interacting.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 232561, "gene_symbol_1": "FOXP3", "gene_symbol_2": "ANXA11", "detection_method": null}} +{"question_id": "PPIL1-0465", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: INSRR (P14616)\n Function: Receptor with tyrosine-protein kinase activity. Functions as a pH sensing receptor which is activated by increased extracellular pH. Activates an intracellular signaling pathway that involves IRS1 and\n Location: Membrane\n\nProtein 2: RABL6 (Q3YEC7)\n Function: Small GTPase involved in the regulation of cell growth and survival (PubMed:19433581). Promotes cellular proliferation (PubMed:16582619, PubMed:19433581). May reduce growth inhibitory activity of CDKN\n Location: Cytoplasm\n\nEvidence: Systematic testing of INSRR and RABL6 in a high-throughput assay did not identify this pair as interacting.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 163211, "gene_symbol_1": "INSRR", "gene_symbol_2": "RABL6", "detection_method": null}} +{"question_id": "PPIL1-0466", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: DZIP1L (Q8IYY4)\n Function: Involved in primary cilium formation (PubMed:19852954, PubMed:28530676). Probably acts as a transition zone protein required for localization of PKD1/PC1 and PKD2/PC2 to the ciliary membrane (PubMed:2\n Location: Cytoplasm, cytoskeleton, cilium basal body\n\nProtein 2: UVRAG (Q9P2Y5)\n Function: Versatile protein that is involved in regulation of different cellular pathways implicated in membrane trafficking. Involved in regulation of the COPI-dependent retrograde transport from Golgi and the\n Location: Late endosome\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair DZIP1L-UVRAG did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 410591, "gene_symbol_1": "DZIP1L", "gene_symbol_2": "UVRAG", "detection_method": null}} +{"question_id": "PPIL1-0467", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: UBE2H (P62256)\n Function: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins (PubMed:17588522, PubMed:20061386, PubMed:8132613). E2 ubiquitin conjugating enzyme that transfers ubiquit\n\nProtein 2: BTF3L4 (Q96K17)\n\nEvidence: A large-scale binary interaction screen systematically tested UBE2H against BTF3L4. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 259138, "gene_symbol_1": "UBE2H", "gene_symbol_2": "BTF3L4", "detection_method": null}} +{"question_id": "PPIL1-0468", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: SEPTIN2 (Q15019)\n Function: Filament-forming cytoskeletal GTPase. Forms a filamentous structure with SEPTIN12, SEPTIN6, SEPTIN2 and probably SEPTIN4 at the sperm annulus which is required for the structural integrity and motilit\n Location: Cytoplasm\n\nProtein 2: CYB561A3 (Q8NBI2)\n Function: Transmembrane reductase that uses ascorbate as an electron donor in the cytoplasm and transfers electrons across membranes to reduce iron cations Fe(3+) into Fe(2+) in the lumen of the late endosome a\n Location: Late endosome membrane\n\nEvidence: Systematic testing of SEPTIN2 and CYB561A3 in a high-throughput assay did not identify this pair as interacting.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 312582, "gene_symbol_1": "SEPTIN2", "gene_symbol_2": "CYB561A3", "detection_method": null}} +{"question_id": "PPIL1-0469", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: CNOT4 (O95628)\n Function: Has E3 ubiquitin ligase activity, promoting ubiquitination and degradation of target proteins (PubMed:11823428, PubMed:22159038, PubMed:26575292). Involved in activation of the JAK/STAT pathway (PubMe\n Location: Cytoplasm\n\nProtein 2: ZNF581 (Q9P0T4)\n Function: May be involved in transcriptional regulation\n Location: Nucleus\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair CNOT4-ZNF581 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 111055, "gene_symbol_1": "CNOT4", "gene_symbol_2": "ZNF581", "detection_method": null}} +{"question_id": "PPIL1-0470", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: PGBD1 (Q96JS3)\n Function: Transposase-derived from PiggyBac DNA transposons. Although it has been fully domesticated and lacks transposase activity, PGBD1 has acquired DNA-binding capability (PubMed:12955498, PubMed:35609796, \n Location: Nucleus\n\nProtein 2: TBC1D23 (Q9NUY8)\n Function: Putative Rab GTPase-activating protein which plays a role in vesicular trafficking (PubMed:28823707). Involved in endosome-to-Golgi trafficking. Acts as a bridging protein by binding simultaneously to\n Location: Golgi apparatus, trans-Golgi network\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair PGBD1-TBC1D23 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 462716, "gene_symbol_1": "PGBD1", "gene_symbol_2": "TBC1D23", "detection_method": null}} +{"question_id": "PPIL1-0471", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: ARL6IP4 (Q66PJ3)\n Function: Involved in modulating alternative pre-mRNA splicing with either 5' distal site activation or preferential use of 3' proximal site. In case of infection by Herpes simplex virus (HSVI), may act as a sp\n Location: Nucleus, nucleolus\n\nProtein 2: ZRANB1 (Q9UGI0)\n Function: Ubiquitin thioesterase, which specifically hydrolyzes 'Lys-29'-linked and 'Lys-33'-linked diubiquitin (PubMed:22157957, PubMed:23827681, PubMed:25752573, PubMed:25752577). Also cleaves 'Lys-63'-linked\n Location: Cytoplasm\n\nEvidence: Systematic testing of ARL6IP4 and ZRANB1 in a high-throughput assay did not identify this pair as interacting.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 359111, "gene_symbol_1": "ARL6IP4", "gene_symbol_2": "ZRANB1", "detection_method": null}} +{"question_id": "PPIL1-0472", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: CD52 (P31358)\n Function: May play a role in carrying and orienting carbohydrate, as well as having a more specific role\n Location: Cell membrane\n\nProtein 2: TBC1D7 (Q9P0N9)\n Function: Non-catalytic component of the TSC-TBC complex, a multiprotein complex that acts as a negative regulator of the canonical mTORC1 complex, an evolutionarily conserved central nutrient sensor that stimu\n Location: Lysosome membrane\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair CD52-TBC1D7 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 201370, "gene_symbol_1": "CD52", "gene_symbol_2": "TBC1D7", "detection_method": null}} +{"question_id": "PPIL1-0473", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: CTSL (P07711)\n Function: Thiol protease important for the overall degradation of proteins in lysosomes (Probable). Plays a critical for normal cellular functions such as general protein turnover, antigen processing and bone r\n Location: Lysosome\n\nProtein 2: OCLN (Q16625)\n Function: May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions\n Location: Cell membrane\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair CTSL-OCLN did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 138100, "gene_symbol_1": "CTSL", "gene_symbol_2": "OCLN", "detection_method": null}} +{"question_id": "PPIL1-0474", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: NICN1 (Q9BSH3)\n Location: Nucleus\n\nProtein 2: UCHL5 (Q9Y5K5)\n Function: Protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains. Deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome. Putative regulatory component of the \n Location: Cytoplasm\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair NICN1-UCHL5 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 476525, "gene_symbol_1": "NICN1", "gene_symbol_2": "UCHL5", "detection_method": null}} +{"question_id": "PPIL1-0475", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: DFFB (O76075)\n Function: Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology\n Location: Cytoplasm\n\nProtein 2: WFDC13 (Q8IUB5)\n Function: Putative acid-stable proteinase inhibitor\n Location: Secreted\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair DFFB-WFDC13 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 97912, "gene_symbol_1": "DFFB", "gene_symbol_2": "WFDC13", "detection_method": null}} +{"question_id": "PPIL1-0476", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: CA1 (P00915)\n Function: Catalyzes the reversible hydration of carbon dioxide (PubMed:10550681, PubMed:16506782, PubMed:16686544, PubMed:16807956, PubMed:17127057, PubMed:17314045, PubMed:17407288, PubMed:18618712, PubMed:191\n Location: Cytoplasm\n\nProtein 2: RPL7L1 (Q6DKI1)\n\nEvidence: A large-scale binary interaction screen systematically tested CA1 against RPL7L1. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 119668, "gene_symbol_1": "CA1", "gene_symbol_2": "RPL7L1", "detection_method": null}} +{"question_id": "PPIL1-0477", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: NHERF2 (Q15599)\n Function: Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expressio\n Location: Endomembrane system\n\nProtein 2: FBF1 (Q8TES7)\n Function: Keratin-binding protein required for epithelial cell polarization. Involved in apical junction complex (AJC) assembly via its interaction with PARD3. Required for ciliogenesis\n Location: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole\n\nEvidence: A large-scale binary interaction screen systematically tested NHERF2 against FBF1. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 318153, "gene_symbol_1": "NHERF2", "gene_symbol_2": "FBF1", "detection_method": null}} +{"question_id": "PPIL1-0478", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: MYL9 (P24844)\n Function: Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping,\n Location: Cytoplasm, cytoskeleton\n\nProtein 2: ODAD2 (Q5T2S8)\n Function: Component of the outer dynein arm-docking complex (ODA-DC) that mediates outer dynein arms (ODA) binding onto the doublet microtubule (PubMed:27486780). Involved in mediating assembly of both ODAs and\n Location: Cytoplasm, cytoskeleton, cilium axoneme\n\nEvidence: Systematic testing of MYL9 and ODAD2 in a high-throughput assay did not identify this pair as interacting.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 187109, "gene_symbol_1": "MYL9", "gene_symbol_2": "ODAD2", "detection_method": null}} +{"question_id": "PPIL1-0479", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: ZCCHC10 (Q8TBK6)\n\nProtein 2: RAPGEF4 (Q8WZA2)\n Function: Guanine nucleotide exchange factor (GEF) for RAP1A, RAP1B and RAP2A small GTPases that is activated by binding cAMP. Does not seem to activate RAB3A. Involved in cAMP-dependent, PKA-independent exocyt\n Location: Cytoplasm\n\nEvidence: Systematic testing of ZCCHC10 and RAPGEF4 in a high-throughput assay did not identify this pair as interacting.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 433504, "gene_symbol_1": "ZCCHC10", "gene_symbol_2": "RAPGEF4", "detection_method": null}} +{"question_id": "PPIL1-0480", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: YES1 (P07947)\n Function: Non-receptor protein tyrosine kinase that is involved in the regulation of cell growth and survival, apoptosis, cell-cell adhesion, cytoskeleton remodeling, and differentiation. Stimulation by recepto\n Location: Cell membrane\n\nProtein 2: ARHGAP17 (Q68EM7)\n Function: Rho GTPase-activating protein involved in the maintenance of tight junction by regulating the activity of CDC42, thereby playing a central role in apical polarity of epithelial cells. Specifically act\n Location: Membrane\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair YES1-ARHGAP17 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 138894, "gene_symbol_1": "YES1", "gene_symbol_2": "ARHGAP17", "detection_method": null}} +{"question_id": "PPIL1-0481", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: HSPE1 (P61604)\n Function: Co-chaperonin implicated in mitochondrial protein import and macromolecular assembly. Together with Hsp60, facilitates the correct folding of imported proteins. May also prevent misfolding and promote\n Location: Mitochondrion matrix\n\nProtein 2: DYDC1 (Q8WWB3)\n Function: Functions as part of axonemal radial spoke complexes that play an important part in the motility of sperm and cilia (By similarity). Plays a crucial role during acrosome biogenesis (PubMed:19545932)\n Location: Cytoplasm, cytoskeleton, flagellum axoneme\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair HSPE1-DYDC1 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 257218, "gene_symbol_1": "HSPE1", "gene_symbol_2": "DYDC1", "detection_method": null}} +{"question_id": "PPIL1-0482", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: HOXB2 (P14652)\n Function: Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis\n Location: Nucleus\n\nProtein 2: CLEC17A (Q6ZS10)\n Function: Cell surface receptor which may be involved in carbohydrate-mediated communication between cells in the germinal center. Binds glycans with terminal alpha-linked mannose or fucose residues\n Location: Membrane\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair HOXB2-CLEC17A did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 163719, "gene_symbol_1": "HOXB2", "gene_symbol_2": "CLEC17A", "detection_method": null}} +{"question_id": "PPIL1-0483", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: VPS33A (Q96AX1)\n Function: Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act as a core component of the putative H\n Location: Cytoplasmic vesicle\n\nProtein 2: RESF1 (Q9HCM1)\n Function: Plays a role in the regulation of imprinted gene expression, regulates repressive epigenetic modifications associated with SETDB1. Required for the recruitment or accumulation of SETDB1 to the endogen\n Location: Nucleus\n\nEvidence: A large-scale binary interaction screen systematically tested VPS33A against RESF1. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 452331, "gene_symbol_1": "VPS33A", "gene_symbol_2": "RESF1", "detection_method": null}} +{"question_id": "PPIL1-0484", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: BMP5 (P22003)\n Function: Growth factor of the TGF-beta superfamily that plays essential roles in many developmental processes, including cartilage and bone formation or neurogenesis (PubMed:11580864, PubMed:29321139). Initiat\n Location: Secreted\n\nProtein 2: PYCR3 (Q53H96)\n Function: Oxidoreductase that catalyzes the last step in proline biosynthesis, which corresponds to the reduction of pyrroline-5-carboxylate (P5C) to L-proline using NAD(P)H (PubMed:23024808, PubMed:36414121). \n Location: Cytoplasm\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair BMP5-PYCR3 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 181530, "gene_symbol_1": "BMP5", "gene_symbol_2": "PYCR3", "detection_method": null}} +{"question_id": "PPIL1-0485", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: RHOA (P61586)\n Function: Small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. Mainly associated with cytoskeleton organization, in active state binds to a variety of effector proteins to regu\n Location: Cell membrane\n\nProtein 2: COPS7B (Q9H9Q2)\n Function: Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathwa\n Location: Cytoplasm\n\nEvidence: A large-scale binary interaction screen systematically tested RHOA against COPS7B. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 256998, "gene_symbol_1": "RHOA", "gene_symbol_2": "COPS7B", "detection_method": null}} +{"question_id": "PPIL1-0486", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: MYD88 (Q99836)\n Function: Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response (PubMed:15361868, PubMed:18292575, PubMed:33718825, PubMed:37971847). Acts via IRAK\n Location: Cytoplasm\n\nProtein 2: CATSPERH (E9PQX1)\n Function: Auxiliary component of the CatSper complex, a complex involved in sperm cell hyperactivation\n Location: Cell projection, cilium, flagellum membrane\n\nEvidence: Systematic testing of MYD88 and CATSPERH in a high-throughput assay did not identify this pair as interacting.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 22797, "gene_symbol_1": "MYD88", "gene_symbol_2": "CATSPERH", "detection_method": null}} +{"question_id": "PPIL1-0487", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: ATN1 (P54259)\n Function: Transcriptional corepressor. Recruits NR2E1 to repress transcription. Promotes vascular smooth cell (VSMC) migration and orientation (By similarity). Corepressor of MTG8 transcriptional repression. Ha\n Location: Nucleus\n\nProtein 2: SEC22A (Q96IW7)\n Function: May be involved in vesicle transport between the ER and the Golgi complex\n Location: Endoplasmic reticulum membrane\n\nEvidence: Systematic testing of ATN1 and SEC22A in a high-throughput assay did not identify this pair as interacting.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 241757, "gene_symbol_1": "ATN1", "gene_symbol_2": "SEC22A", "detection_method": null}} +{"question_id": "PPIL1-0488", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: KATNAL1 (Q9BW62)\n Function: Regulates microtubule dynamics in Sertoli cells, a process that is essential for spermiogenesis and male fertility. Severs microtubules in an ATP-dependent manner, promoting rapid reorganization of ce\n Location: Cytoplasm, cytoskeleton\n\nProtein 2: PARP3 (Q9Y6F1)\n Function: Mono-ADP-ribosyltransferase that mediates mono-ADP-ribosylation of target proteins and plays a key role in the response to DNA damage (PubMed:16924674, PubMed:19354255, PubMed:20064938, PubMed:2121172\n Location: Nucleus\n\nEvidence: A large-scale binary interaction screen systematically tested KATNAL1 against PARP3. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 479406, "gene_symbol_1": "KATNAL1", "gene_symbol_2": "PARP3", "detection_method": null}} +{"question_id": "PPIL1-0489", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: WDR62 (O43379)\n Function: Required for cerebral cortical development. Plays a role in neuronal proliferation and migration (PubMed:20729831, PubMed:20890278). Plays a role in mother-centriole-dependent centriole duplication; t\n Location: Nucleus\n\nProtein 2: PUDP (Q08623)\n Function: Dephosphorylates pseudouridine 5'-phosphate, a potential intermediate in rRNA degradation. Pseudouridine is then excreted intact in urine\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair WDR62-PUDP did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 60073, "gene_symbol_1": "WDR62", "gene_symbol_2": "PUDP", "detection_method": null}} +{"question_id": "PPIL1-0490", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: TMF1 (P82094)\n Function: Potential coactivator of the androgen receptor. Mediates STAT3 degradation. May play critical roles in two RAB6-dependent retrograde transport processes: one from endosomes to the Golgi and the other \n Location: Cytoplasm\n\nProtein 2: GAS2L3 (Q86XJ1)\n Function: Cytoskeletal linker protein. May promote and stabilize the formation of the actin and microtubule network\n Location: Cytoplasm\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair TMF1-GAS2L3 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 269071, "gene_symbol_1": "TMF1", "gene_symbol_2": "GAS2L3", "detection_method": null}} +{"question_id": "PPIL1-0491", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: KLHDC7B (A0A3B3ISF6)\n\nProtein 2: TCEA2 (Q15560)\n Function: Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA \n Location: Nucleus\n\nEvidence: Systematic testing of KLHDC7B and TCEA2 in a high-throughput assay did not identify this pair as interacting.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 3869, "gene_symbol_1": "KLHDC7B", "gene_symbol_2": "TCEA2", "detection_method": null}} +{"question_id": "PPIL1-0492", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: RFC4 (P35249)\n Function: Subunit of the replication factor C (RFC) complex which acts during elongation of primed DNA templates by DNA polymerases delta and epsilon, and is necessary for ATP-dependent loading of proliferating\n Location: Nucleus\n\nProtein 2: FNTB (P49356)\n Function: Essential subunit of the farnesyltransferase complex. Catalyzes the transfer of a farnesyl moiety from farnesyl diphosphate to a cysteine at the fourth position from the C-terminus of several proteins\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair RFC4-FNTB did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 206463, "gene_symbol_1": "RFC4", "gene_symbol_2": "FNTB", "detection_method": null}} +{"question_id": "PPIL1-0493", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: CCL16 (O15467)\n Function: Shows chemotactic activity for lymphocytes and monocytes but not neutrophils. Also shows potent myelosuppressive activity, suppresses proliferation of myeloid progenitor cells. Recombinant SCYA16 show\n Location: Secreted\n\nProtein 2: LTO1 (Q8WV07)\n Function: The complex LTO1:YAE1 functions as a target specific adapter that probably recruits apo-ABCE1 to the cytosolic iron-sulfur protein assembly (CIA) complex machinery (PubMed:26182403). May be required f\n Location: Nucleus\n\nEvidence: A large-scale binary interaction screen systematically tested CCL16 against LTO1. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 53994, "gene_symbol_1": "CCL16", "gene_symbol_2": "LTO1", "detection_method": null}} +{"question_id": "PPIL1-0494", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: CLHC1 (Q8NHS4)\n\nProtein 2: SNIP1 (Q8TAD8)\n Function: Required for pre-mRNA splicing as component of the spliceosome (PubMed:29360106). As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs (Probable). Down-re\n Location: Nucleus\n\nEvidence: A large-scale binary interaction screen systematically tested CLHC1 against SNIP1. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 430574, "gene_symbol_1": "CLHC1", "gene_symbol_2": "SNIP1", "detection_method": null}} +{"question_id": "PPIL1-0495", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: CMTM5 (Q96DZ9)\n Location: Membrane\n\nProtein 2: SEC23IP (Q9Y6Y8)\n Function: Plays a role in the organization of endoplasmic reticulum exit sites. Specifically binds to phosphatidylinositol 3-phosphate (PI(3)P), phosphatidylinositol 4-phosphate (PI(4)P) and phosphatidylinosito\n Location: Cytoplasmic vesicle, COPII-coated vesicle membrane\n\nEvidence: A large-scale binary interaction screen systematically tested CMTM5 against SEC23IP. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 456379, "gene_symbol_1": "CMTM5", "gene_symbol_2": "SEC23IP", "detection_method": null}} +{"question_id": "PPIL1-0496", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: TWSG1 (Q9GZX9)\n Function: May be involved in dorsoventral axis formation. Seems to antagonize BMP signaling by forming ternary complexes with CHRD and BMPs, thereby preventing BMPs from binding to their receptors. In addition \n Location: Secreted\n\nProtein 2: LYAR (Q9NX58)\n Function: Plays a role in the maintenance of the appropriate processing of 47S/45S pre-rRNA to 32S/30S pre-rRNAs and their subsequent processing to produce 18S and 28S rRNAs (PubMed:24495227). Also acts at the \n Location: Nucleus\n\nEvidence: A large-scale binary interaction screen systematically tested TWSG1 against LYAR. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 484113, "gene_symbol_1": "TWSG1", "gene_symbol_2": "LYAR", "detection_method": null}} +{"question_id": "PPIL1-0497", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: ERCC3 (P19447)\n Function: ATP-dependent 3'-5' DNA helicase/translocase (PubMed:17466626, PubMed:27193682, PubMed:33902107, PubMed:8465201, PubMed:8663148). Binds dsDNA rather than ssDNA, unzipping it in a translocase rather th\n Location: Nucleus\n\nProtein 2: ZNF664 (Q8N3J9)\n Function: May be involved in transcriptional regulation\n Location: Nucleus\n\nEvidence: A large-scale binary interaction screen systematically tested ERCC3 against ZNF664. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 175793, "gene_symbol_1": "ERCC3", "gene_symbol_2": "ZNF664", "detection_method": null}} +{"question_id": "PPIL1-0498", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: TAT (P17735)\n Function: Transaminase involved in tyrosine breakdown. Converts tyrosine to p-hydroxyphenylpyruvate. Can catalyze the reverse reaction, using glutamic acid, with 2-oxoglutarate as cosubstrate (in vitro). Has mu\n\nProtein 2: FCRL2 (Q96LA5)\n Function: May have an regulatory role in normal and neoplastic B cell development\n Location: Cell membrane\n\nEvidence: A large-scale binary interaction screen systematically tested TAT against FCRL2. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 172895, "gene_symbol_1": "TAT", "gene_symbol_2": "FCRL2", "detection_method": null}} +{"question_id": "PPIL1-0499", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: ATP6V1E2 (Q96A05)\n Function: Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates prot\n\nProtein 2: PIGU (Q9H490)\n Function: Component of the glycosylphosphatidylinositol-anchor (GPI-anchor) transamidase (GPI-T) complex that catalyzes the formation of the linkage between a proprotein and a GPI-anchor and participates in GPI\n Location: Endoplasmic reticulum membrane\n\nEvidence: Systematic testing of ATP6V1E2 and PIGU in a high-throughput assay did not identify this pair as interacting.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 451059, "gene_symbol_1": "ATP6V1E2", "gene_symbol_2": "PIGU", "detection_method": null}} +{"question_id": "PPIL1-0500", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: CDC16 (Q13042)\n Function: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle (PubMed:18485873)\n Location: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome\n\nProtein 2: FNDC9 (Q8TBE3)\n Location: Membrane\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair CDC16-FNDC9 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 291021, "gene_symbol_1": "CDC16", "gene_symbol_2": "FNDC9", "detection_method": null}} +{"question_id": "PPIL1-0501", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: GSTM2 (P28161)\n Function: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Participates in the formation of novel hepoxilin regioisomers (PubMed:21046276)\n Location: Cytoplasm\n\nProtein 2: BMAL2 (Q8WYA1)\n Function: Transcriptional activator which forms a core component of the circadian clock. The circadian clock, an internal time-keeping system, regulates various physiological processes through the generation of\n Location: Nucleus\n\nEvidence: A large-scale binary interaction screen systematically tested GSTM2 against BMAL2. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 193608, "gene_symbol_1": "GSTM2", "gene_symbol_2": "BMAL2", "detection_method": null}} +{"question_id": "PPIL1-0502", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: SLC43A1 (O75387)\n Function: Uniport that mediates the transport of neutral amino acids such as L-leucine, L-isoleucine, L-valine, and L-phenylalanine (PubMed:12930836). The transport activity is sodium ions-independent, electron\n Location: Cell membrane\n\nProtein 2: MTRR (Q9UBK8)\n Function: Key enzyme in methionine and folate homeostasis responsible for the reactivation of methionine synthase (MTR/MS) activity by catalyzing the reductive methylation of MTR-bound cob(II)alamin (PubMed:178\n Location: Cytoplasm\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair SLC43A1-MTRR did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 86314, "gene_symbol_1": "SLC43A1", "gene_symbol_2": "MTRR", "detection_method": null}} +{"question_id": "PPIL1-0503", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: ZWINT (O95229)\n Function: Acts as a component of the outer kinetochore KNL1 complex that serves as a docking point for spindle assembly checkpoint components and mediates microtubule-kinetochore interactions (PubMed:15094189, \n Location: Nucleus\n\nProtein 2: VKORC1L1 (Q8N0U8)\n Function: Involved in vitamin K metabolism. Can reduce inactive vitamin K 2,3-epoxide to active vitamin K, and may contribute to vitamin K-mediated protection against oxidative stress. Plays a role in vitamin K\n Location: Endoplasmic reticulum membrane\n\nEvidence: A large-scale binary interaction screen systematically tested ZWINT against VKORC1L1. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 105186, "gene_symbol_1": "ZWINT", "gene_symbol_2": "VKORC1L1", "detection_method": null}} +{"question_id": "PPIL1-0504", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: CYP1A2 (P05177)\n Function: A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins (PubMed:10681376, PubMed:11555828, PubMed:12865317, Pu\n Location: Endoplasmic reticulum membrane\n\nProtein 2: CCDC120 (Q96HB5)\n Function: Centriolar protein required for centriole subdistal appendage assembly and microtubule anchoring in interphase cells (PubMed:28422092). Together with CCDC68, cooperate with subdistal appendage compone\n Location: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole\n\nEvidence: A large-scale binary interaction screen systematically tested CYP1A2 against CCDC120. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 131867, "gene_symbol_1": "CYP1A2", "gene_symbol_2": "CCDC120", "detection_method": null}} +{"question_id": "PPIL1-0505", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: PAK1 (Q13153)\n Function: Protein kinase involved in intracellular signaling pathways downstream of integrins and receptor-type kinases that plays an important role in cytoskeleton dynamics, in cell adhesion, migration, prolif\n Location: Cytoplasm\n\nProtein 2: LDB3 (O75112)\n Function: May function as an adapter in striated muscle to couple protein kinase C-mediated signaling via its LIM domains to the cytoskeleton\n Location: Cytoplasm, perinuclear region\n\nEvidence: A large-scale binary interaction screen systematically tested PAK1 against LDB3. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 82701, "gene_symbol_1": "PAK1", "gene_symbol_2": "LDB3", "detection_method": null}} +{"question_id": "PPIL1-0506", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: N4BP1 (O75113)\n Function: Potent suppressor of cytokine production that acts as a regulator of innate immune signaling and inflammation. Acts as a key negative regulator of select cytokine and chemokine responses elicited by T\n Location: Cytoplasm, cytosol\n\nProtein 2: TRMT2A (Q8IZ69)\n Function: S-adenosyl-L-methionine-dependent methyltransferase that catalyzes the formation of 5-methyl-uridine in tRNAs and some mRNAs (PubMed:31361898, PubMed:33799331, PubMed:34556860). Mainly catalyzes the m\n Location: Cytoplasm, cytosol\n\nEvidence: A large-scale binary interaction screen systematically tested N4BP1 against TRMT2A. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 82843, "gene_symbol_1": "N4BP1", "gene_symbol_2": "TRMT2A", "detection_method": null}} +{"question_id": "PPIL1-0507", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: PPP1R18 (Q6NYC8)\n Function: May target protein phosphatase 1 to F-actin cytoskeleton\n Location: Cytoplasm, cytoskeleton\n\nProtein 2: PPP1R16B (Q96T49)\n Function: Regulator of protein phosphatase 1 (PP1) that acts as a positive regulator of pulmonary endothelial cell (EC) barrier function (PubMed:18586956). Involved in the regulation of the PI3K/AKT signaling p\n Location: Cell membrane\n\nEvidence: Systematic testing of PPP1R18 and PPP1R16B in a high-throughput assay did not identify this pair as interacting.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 367755, "gene_symbol_1": "PPP1R18", "gene_symbol_2": "PPP1R16B", "detection_method": null}} +{"question_id": "PPIL1-0508", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: MAGEA1 (P43355)\n Function: May be involved in transcriptional regulation through interaction with SNW1 and recruiting histone deactelyase HDAC1. May inhibit notch intracellular domain (NICD) transactivation. May play a role in \n Location: Cytoplasm\n\nProtein 2: RNF214 (Q8ND24)\n\nEvidence: A large-scale binary interaction screen systematically tested MAGEA1 against RNF214. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 217623, "gene_symbol_1": "MAGEA1", "gene_symbol_2": "RNF214", "detection_method": null}} +{"question_id": "PPIL1-0509", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: CMPK1 (P30085)\n Function: Catalyzes the phosphorylation of pyrimidine nucleoside monophosphates at the expense of ATP. Plays an important role in de novo pyrimidine nucleotide biosynthesis. Has preference for UMP and CMP as ph\n Location: Nucleus\n\nProtein 2: CHST13 (Q8NET6)\n Function: Catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is d\n Location: Golgi apparatus membrane\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair CMPK1-CHST13 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 197733, "gene_symbol_1": "CMPK1", "gene_symbol_2": "CHST13", "detection_method": null}} +{"question_id": "PPIL1-0510", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: IL13RA2 (Q14627)\n Function: Cell surface receptor that plays a role in the regulation of IL-13-mediated responses (PubMed:11861389, PubMed:17030238). Functions as a decoy receptor that inhibits IL-13- and IL-4-mediated signal tr\n Location: Cell membrane\n\nProtein 2: DKK2 (Q9UBU2)\n Function: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play \n Location: Secreted\n\nEvidence: A large-scale binary interaction screen systematically tested IL13RA2 against DKK2. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 308433, "gene_symbol_1": "IL13RA2", "gene_symbol_2": "DKK2", "detection_method": null}} +{"question_id": "PPIL1-0511", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: DENR (O43583)\n Function: Translation regulator forming a complex with MCTS1 to promote translation reinitiation. Translation reinitiation is the process where the small ribosomal subunit remains attached to the mRNA following\n Location: Cytoplasm\n\nProtein 2: PRL (P01236)\n Function: Prolactin acts primarily on the mammary gland by promoting lactation\n Location: Secreted\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair DENR-PRL did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 63140, "gene_symbol_1": "DENR", "gene_symbol_2": "PRL", "detection_method": null}} +{"question_id": "PPIL1-0512", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: TRIP10 (Q15642)\n Function: Required for translocation of GLUT4 to the plasma membrane in response to insulin signaling (By similarity). Required to coordinate membrane tubulation with reorganization of the actin cytoskeleton du\n Location: Cytoplasm, cytoskeleton\n\nProtein 2: GSG1L (Q6UXU4)\n Function: As a component of the inner core of AMPAR complex, modifies AMPA receptor (AMPAR) gating\n Location: Cell membrane\n\nEvidence: Systematic testing of TRIP10 and GSG1L in a high-throughput assay did not identify this pair as interacting.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 318525, "gene_symbol_1": "TRIP10", "gene_symbol_2": "GSG1L", "detection_method": null}} +{"question_id": "PPIL1-0513", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: FMR1 (Q06787)\n Function: Multifunctional polyribosome-associated RNA-binding protein that plays a central role in neuronal development and synaptic plasticity through the regulation of alternative mRNA splicing, mRNA stabilit\n Location: Cytoplasm, Cytoplasmic ribonucleoprotein granule\n\nProtein 2: KDF1 (Q8NAX2)\n Function: Plays a role in the regulation of the epidermis formation during early development. Required both as an inhibitor of basal cell proliferation and a promoter of differentiation of basal progenitor cell\n Location: Cytoplasm\n\nEvidence: Systematic testing of FMR1 and KDF1 in a high-throughput assay did not identify this pair as interacting.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 281907, "gene_symbol_1": "FMR1", "gene_symbol_2": "KDF1", "detection_method": null}} +{"question_id": "PPIL1-0514", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: MT1E (P04732)\n Function: Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids\n\nProtein 2: ATG5 (Q9H1Y0)\n Function: Involved in autophagic vesicle formation. Conjugation with ATG12, through a ubiquitin-like conjugating system involving ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme,\n Location: Cytoplasm\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair MT1E-ATG5 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 129698, "gene_symbol_1": "MT1E", "gene_symbol_2": "ATG5", "detection_method": null}} +{"question_id": "PPIL1-0515", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: RIIAD1 (A6NNX1)\n\nProtein 2: TSKS (Q9UJT2)\n Function: May play a role in testicular physiology, most probably in the process of spermatogenesis or spermatid development\n Location: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole\n\nEvidence: A large-scale binary interaction screen systematically tested RIIAD1 against TSKS. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 16041, "gene_symbol_1": "RIIAD1", "gene_symbol_2": "TSKS", "detection_method": null}} +{"question_id": "PPIL1-0516", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: HIP1 (O00291)\n Function: Plays a role in clathrin-mediated endocytosis and trafficking (PubMed:11532990, PubMed:11577110, PubMed:11889126). Involved in regulating AMPA receptor trafficking in the central nervous system in an \n Location: Cytoplasm\n\nProtein 2: PROP1 (O75360)\n Function: Possibly involved in the ontogenesis of pituitary gonadotropes, as well as somatotropes, lactotropes and caudomedial thyrotropes\n Location: Nucleus\n\nEvidence: A large-scale binary interaction screen systematically tested HIP1 against PROP1. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 28735, "gene_symbol_1": "HIP1", "gene_symbol_2": "PROP1", "detection_method": null}} +{"question_id": "PPIL1-0517", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: HSBP1L1 (C9JCN9)\n\nProtein 2: C5orf24 (Q7Z6I8)\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair HSBP1L1-C5orf24 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 21689, "gene_symbol_1": "HSBP1L1", "gene_symbol_2": "C5orf24", "detection_method": null}} +{"question_id": "PPIL1-0518", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: DES (P17661)\n Function: Muscle-specific type III intermediate filament essential for proper muscular structure and function. Plays a crucial role in maintaining the structure of sarcomeres, inter-connecting the Z-disks and f\n Location: Cytoplasm, myofibril, sarcomere, Z line\n\nProtein 2: LYG2 (Q86SG7)\n Function: May act as a potent antibacterial protein that may play a role in the innate immunity\n Location: Secreted\n\nEvidence: Systematic testing of DES and LYG2 in a high-throughput assay did not identify this pair as interacting.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 172689, "gene_symbol_1": "DES", "gene_symbol_2": "LYG2", "detection_method": null}} +{"question_id": "PPIL1-0519", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: ZKSCAN3 (Q9BRR0)\n Function: Transcriptional factor that binds to the consensus sequence 5'-[GT][AG][AGT]GGGG-3' and acts as a repressor of autophagy. Specifically represses expression of genes involved in autophagy and lysosome \n Location: Nucleus\n\nProtein 2: PCYOX1 (Q9UHG3)\n Function: Prenylcysteine oxidase that cleaves the thioether bond of prenyl-L-cysteines, such as farnesylcysteine and geranylgeranylcysteine (PubMed:10585463, PubMed:11078725, PubMed:12186880). Only active again\n Location: Lysosome\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair ZKSCAN3-PCYOX1 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 475876, "gene_symbol_1": "ZKSCAN3", "gene_symbol_2": "PCYOX1", "detection_method": null}} +{"question_id": "PPIL1-0520", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: MID1 (O15344)\n Function: Has E3 ubiquitin ligase activity towards IGBP1, promoting its monoubiquitination, which results in deprotection of the catalytic subunit of protein phosphatase PP2A, and its subsequent degradation by \n Location: Cytoplasm\n\nProtein 2: ZNF230 (Q9UIE0)\n Function: May be involved in transcriptional regulation\n Location: Nucleus\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair MID1-ZNF230 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 51772, "gene_symbol_1": "MID1", "gene_symbol_2": "ZNF230", "detection_method": null}} +{"question_id": "PPIL1-0521", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: C2orf74 (A8MZ97)\n Location: Membrane\n\nProtein 2: BATF3 (Q9NR55)\n Function: AP-1 family transcription factor that controls the differentiation of CD8(+) thymic conventional dendritic cells in the immune system. Required for development of CD8-alpha(+) classical dendritic cell\n Location: Nucleus\n\nEvidence: Systematic testing of C2orf74 and BATF3 in a high-throughput assay did not identify this pair as interacting.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 19129, "gene_symbol_1": "C2orf74", "gene_symbol_2": "BATF3", "detection_method": null}} +{"question_id": "PPIL1-0522", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: SS18 (Q15532)\n Function: Appears to function synergistically with RBM14 as a transcriptional coactivator. Isoform 1 and isoform 2 function in nuclear receptor coactivation. Isoform 1 and isoform 2 function in general transcri\n Location: Nucleus\n\nProtein 2: LCE1E (Q5T753)\n Function: Precursors of the cornified envelope of the stratum corneum\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair SS18-LCE1E did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 317359, "gene_symbol_1": "SS18", "gene_symbol_2": "LCE1E", "detection_method": null}} +{"question_id": "PPIL1-0523", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: STX1A (Q16623)\n Function: Plays an essential role in hormone and neurotransmitter calcium-dependent exocytosis and endocytosis (PubMed:26635000). Part of the SNARE (Soluble NSF Attachment Receptor) complex composed of SNAP25, \n Location: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane\n\nProtein 2: ZNF513 (Q8N8E2)\n Function: Transcriptional regulator that plays a role in retinal development and maintenance\n Location: Nucleus\n\nEvidence: A large-scale binary interaction screen systematically tested STX1A against ZNF513. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 325151, "gene_symbol_1": "STX1A", "gene_symbol_2": "ZNF513", "detection_method": null}} +{"question_id": "PPIL1-0524", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: CAMK1 (Q14012)\n Function: Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK1 signaling cascade and, upon calcium influx, regulates transcription activators activity, cell cycle, horm\n Location: Cytoplasm\n\nProtein 2: LRRC18 (Q8N456)\n Function: May be involved in the regulation of spermatogenesis and sperm maturation\n Location: Cytoplasm\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair CAMK1-LRRC18 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 302656, "gene_symbol_1": "CAMK1", "gene_symbol_2": "LRRC18", "detection_method": null}} +{"question_id": "PPIL1-0525", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: ZC2HC1C (Q53FD0)\n\nProtein 2: CCDC42 (Q96M95)\n Function: Essential for male fertility. Required for sperm development\n Location: Cytoplasm, perinuclear region\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair ZC2HC1C-CCDC42 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 339523, "gene_symbol_1": "ZC2HC1C", "gene_symbol_2": "CCDC42", "detection_method": null}} +{"question_id": "PPIL1-0526", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: IGF2BP3 (O00425)\n Function: RNA-binding factor that may recruit target transcripts to cytoplasmic protein-RNA complexes (mRNPs). This transcript 'caging' into mRNPs allows mRNA transport and transient storage. It also modulates \n Location: Nucleus\n\nProtein 2: STX12 (Q86Y82)\n Function: SNARE promoting fusion of transport vesicles with target membranes. Together with SNARE STX6, promotes movement of vesicles from endosomes to the cell membrane, and may therefore function in the endoc\n Location: Endosome membrane\n\nEvidence: Systematic testing of IGF2BP3 and STX12 in a high-throughput assay did not identify this pair as interacting.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 30284, "gene_symbol_1": "IGF2BP3", "gene_symbol_2": "STX12", "detection_method": null}} +{"question_id": "PPIL1-0527", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: PGLS (O95336)\n Function: Hydrolysis of 6-phosphogluconolactone to 6-phosphogluconate\n Location: Cytoplasm\n\nProtein 2: TMEM60 (Q9H2L4)\n Location: Membrane\n\nEvidence: A large-scale binary interaction screen systematically tested PGLS against TMEM60. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 106896, "gene_symbol_1": "PGLS", "gene_symbol_2": "TMEM60", "detection_method": null}} +{"question_id": "PPIL1-0528", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: NR0B1 (P51843)\n Function: Nuclear receptor that lacks a DNA-binding domain and acts as a corepressor that inhibits the transcriptional activity of other nuclear receptors through heterodimeric interactions (PubMed:12482977, Pu\n Location: Nucleus\n\nProtein 2: SGTB (Q96EQ0)\n Function: Co-chaperone that binds directly to HSC70 and HSP70 and regulates their ATPase activity\n\nEvidence: A large-scale binary interaction screen systematically tested NR0B1 against SGTB. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 235744, "gene_symbol_1": "NR0B1", "gene_symbol_2": "SGTB", "detection_method": null}} +{"question_id": "PPIL1-0529", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: DAPL1 (A0PJW8)\n Function: May play a role in the early stages of epithelial differentiation or in apoptosis\n\nProtein 2: ALG10B (Q5I7T1)\n Function: Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine\n Location: Endoplasmic reticulum membrane\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair DAPL1-ALG10B did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 5983, "gene_symbol_1": "DAPL1", "gene_symbol_2": "ALG10B", "detection_method": null}} +{"question_id": "PPIL1-0530", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: GFUS (Q13630)\n Function: Catalyzes the two-step NADP-dependent conversion of GDP-4-dehydro-6-deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction\n\nProtein 2: TMEM47 (Q9BQJ4)\n Function: Regulates cell junction organization in epithelial cells. May play a role in the transition from adherens junction to tight junction assembly. May regulate F-actin polymerization required for tight ju\n Location: Membrane\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair GFUS-TMEM47 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 300451, "gene_symbol_1": "GFUS", "gene_symbol_2": "TMEM47", "detection_method": null}} +{"question_id": "PPIL1-0531", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: NKX2-4 (Q9H2Z4)\n Function: Probable transcription factor\n Location: Nucleus\n\nProtein 2: ACTR10 (Q9NZ32)\n Function: Part of the dynactin complex that activates the molecular motor dynein for ultra-processive transport along microtubules\n Location: Cytoplasm, cytoskeleton\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair NKX2-4-ACTR10 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 486569, "gene_symbol_1": "NKX2-4", "gene_symbol_2": "ACTR10", "detection_method": null}} +{"question_id": "PPIL1-0532", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: ZC3H11A (O75152)\n Function: Through its association with TREX complex components, may participate in the export and post-transcriptional coordination of selected mRNA transcripts, including those required to maintain the metabol\n Location: Nucleus\n\nProtein 2: TSPAN18 (Q96SJ8)\n Function: Plays a role in the cell surface localization of ORAI1 and may participate in the regulation of Ca(2+) signaling and the VWF release in response to inflammatory stimuli\n Location: Membrane\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair ZC3H11A-TSPAN18 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 83919, "gene_symbol_1": "ZC3H11A", "gene_symbol_2": "TSPAN18", "detection_method": null}} +{"question_id": "PPIL1-0533", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: RENBP (P51606)\n Function: Catalyzes the interconversion of N-acetylglucosamine to N-acetylmannosamine (PubMed:10502668, PubMed:12499362, PubMed:9990133). Involved in the N-glycolylneuraminic acid (Neu5Gc) degradation pathway: \n\nProtein 2: PLCB4 (Q15147)\n Function: Activated phosphatidylinositol-specific phospholipase C enzymes catalyze the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) involved in G-prot\n Location: Cell membrane\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair RENBP-PLCB4 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 234156, "gene_symbol_1": "RENBP", "gene_symbol_2": "PLCB4", "detection_method": null}} +{"question_id": "PPIL1-0534", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: SLC31A2 (O15432)\n Function: Does not function as a copper(1+) importer in vivo (By similarity). However, in vitro functions as a low-affinity copper(1+) importer (PubMed:17617060, PubMed:17944601). Regulator of SLC31A1 which fac\n Location: Membrane\n\nProtein 2: PEA15 (Q15121)\n Function: Blocks Ras-mediated inhibition of integrin activation and modulates the ERK MAP kinase cascade. Inhibits RPS6KA3 activities by retaining it in the cytoplasm (By similarity). Inhibits both TNFRSF6- and\n Location: Cytoplasm\n\nEvidence: Systematic testing of SLC31A2 and PEA15 in a high-throughput assay did not identify this pair as interacting.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 53550, "gene_symbol_1": "SLC31A2", "gene_symbol_2": "PEA15", "detection_method": null}} +{"question_id": "PPIL1-0535", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: NTNG2 (Q96CW9)\n Function: Involved in controlling patterning and neuronal circuit formation at the laminar, cellular, subcellular and synaptic levels. Promotes neurite outgrowth of both axons and dendrites\n Location: Cell membrane\n\nProtein 2: HYPK (Q9NX55)\n Function: Component of several N-terminal acetyltransferase complexes (PubMed:20154145, PubMed:29754825, PubMed:32042062). Inhibits the N-terminal acetylation activity of the N-terminal acetyltransferase NAA10-\n Location: Nucleus\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair NTNG2-HYPK did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 454979, "gene_symbol_1": "NTNG2", "gene_symbol_2": "HYPK", "detection_method": null}} +{"question_id": "PPIL1-0536", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: GNAI2 (P04899)\n Function: Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades (PubMed:38505600, PubMed:29925945, PubMed:3563\n Location: Cytoplasm\n\nProtein 2: FAM153A (Q9UHL3)\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair GNAI2-FAM153A did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 130288, "gene_symbol_1": "GNAI2", "gene_symbol_2": "FAM153A", "detection_method": null}} +{"question_id": "PPIL1-0537", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: PBLD (P30039)\n Function: Functions as a positive regulator of the antiviral immune response by modulating innate immunity and viral replication by enhancing type I interferon (IFN-I) response (PubMed:39362857, PubMed:39589880\n\nProtein 2: PHLDA1 (Q8WV24)\n Function: Seems to be involved in regulation of apoptosis. May be involved in detachment-mediated programmed cell death. May mediate apoptosis during neuronal development. May be involved in regulation of anti-\n Location: Cytoplasm\n\nEvidence: A large-scale binary interaction screen systematically tested PBLD against PHLDA1. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 197060, "gene_symbol_1": "PBLD", "gene_symbol_2": "PHLDA1", "detection_method": null}} +{"question_id": "PPIL1-0538", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: TRIM72 (Q6ZMU5)\n Function: Muscle-specific E3 ubiquitin-protein ligase that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at injury sites (PubMed:36944613). Its ubiquitination a\n Location: Cell membrane, sarcolemma\n\nProtein 2: FAN1 (Q9Y2M0)\n Function: Nuclease required for the repair of DNA interstrand cross-links (ICL) recruited at sites of DNA damage by monoubiquitinated FANCD2. Specifically involved in repair of ICL-induced DNA breaks by being r\n Location: Nucleus\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair TRIM72-FAN1 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 379388, "gene_symbol_1": "TRIM72", "gene_symbol_2": "FAN1", "detection_method": null}} +{"question_id": "PPIL1-0539", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: PYGM (P11217)\n Function: Allosteric enzyme that catalyzes the rate-limiting step in glycogen catabolism, the phosphorolytic cleavage of glycogen to produce glucose-1-phosphate, and plays a central role in maintaining cellular\n\nProtein 2: PALS2 (Q9NZW5)\n Location: Membrane\n\nEvidence: Systematic testing of PYGM and PALS2 in a high-throughput assay did not identify this pair as interacting.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 155906, "gene_symbol_1": "PYGM", "gene_symbol_2": "PALS2", "detection_method": null}} +{"question_id": "PPIL1-0540", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: ELMOD3 (Q96FG2)\n Function: Acts as a GTPase-activating protein (GAP) for ARL2 with low specific activity\n Location: Cell projection, stereocilium\n\nProtein 2: FUBP3 (Q96I24)\n Function: May interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression\n Location: Nucleus\n\nEvidence: A large-scale binary interaction screen systematically tested ELMOD3 against FUBP3. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 458252, "gene_symbol_1": "ELMOD3", "gene_symbol_2": "FUBP3", "detection_method": null}} +{"question_id": "PPIL1-0541", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: TRAPPC14 (Q8WVR3)\n Function: Specific subunit of the TRAPP (transport protein particle) II complex, a highly conserved vesicle tethering complex that functions in late Golgi trafficking as a membrane tether (PubMed:30715179, PubM\n Location: Cytoplasm, cytoskeleton, spindle\n\nProtein 2: CNKSR1 (Q969H4)\n Function: May function as an adapter protein or regulator of Ras signaling pathways\n Location: Cytoplasm\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair TRAPPC14-CNKSR1 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 440771, "gene_symbol_1": "TRAPPC14", "gene_symbol_2": "CNKSR1", "detection_method": null}} +{"question_id": "PPIL1-0542", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: SLC16A3 (O15427)\n Function: Proton-dependent transporter of monocarboxylates such as L-lactate and pyruvate (PubMed:11101640, PubMed:23935841, PubMed:31719150). Plays a predominant role in L-lactate efflux from highly glycolytic\n Location: Cell membrane\n\nProtein 2: MYOG (P15173)\n Function: Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation, cell cycle exit and muscle atrophy. Essential for the develo\n Location: Nucleus\n\nEvidence: A large-scale binary interaction screen systematically tested SLC16A3 against MYOG. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 53399, "gene_symbol_1": "SLC16A3", "gene_symbol_2": "MYOG", "detection_method": null}} +{"question_id": "PPIL1-0543", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: EIF4E2 (O60573)\n Function: Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation. Acts as a repressor of translation initiation (PubMed:17368478, PubMed:22751931, PubMed:25624349,\n Location: Cytoplasm\n\nProtein 2: DUSP13B (Q9UII6)\n Function: Dual specificity phosphatase that dephosphorylates MAPK8/JNK and MAPK14/p38, but not MAPK1/ERK2, in vitro (PubMed:21360282). Exhibits intrinsic phosphatase activity towards both phospho-seryl/threonyl\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair EIF4E2-DUSP13B did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 75835, "gene_symbol_1": "EIF4E2", "gene_symbol_2": "DUSP13B", "detection_method": null}} +{"question_id": "PPIL1-0544", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: CERK (Q8TCT0)\n Function: Catalyzes specifically the phosphorylation of ceramide to form ceramide 1-phosphate (PubMed:11956206, PubMed:16269826, PubMed:19168031). Acts efficiently on natural and analog ceramides (C6, C8, C16 c\n Location: Cytoplasm\n\nProtein 2: BTF3L4 (Q96K17)\n\nEvidence: A large-scale binary interaction screen systematically tested CERK against BTF3L4. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 435085, "gene_symbol_1": "CERK", "gene_symbol_2": "BTF3L4", "detection_method": null}} +{"question_id": "PPIL1-0545", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: COG7 (P83436)\n Function: Required for normal Golgi function\n Location: Golgi apparatus membrane\n\nProtein 2: PPFIBP2 (Q8ND30)\n Function: May regulate the disassembly of focal adhesions. Did not bind receptor-like tyrosine phosphatases type 2A\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair COG7-PPFIBP2 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 269700, "gene_symbol_1": "COG7", "gene_symbol_2": "PPFIBP2", "detection_method": null}} +{"question_id": "PPIL1-0546", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: OR5H1 (A6NKK0)\n Function: Odorant receptor\n Location: Cell membrane\n\nProtein 2: TMEM44 (Q2T9K0)\n Location: Membrane\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair OR5H1-TMEM44 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 15030, "gene_symbol_1": "OR5H1", "gene_symbol_2": "TMEM44", "detection_method": null}} +{"question_id": "PPIL1-0547", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: NDUFA7 (O95182)\n Function: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons fro\n Location: Mitochondrion inner membrane\n\nProtein 2: SYNGAP1 (Q96PV0)\n Function: Major constituent of the PSD essential for postsynaptic signaling. Inhibitory regulator of the Ras-cAMP pathway. Member of the NMDAR signaling complex in excitatory synapses, it may play a role in NMD\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair NDUFA7-SYNGAP1 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 103927, "gene_symbol_1": "NDUFA7", "gene_symbol_2": "SYNGAP1", "detection_method": null}} +{"question_id": "PPIL1-0548", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: PRR22 (Q8IZ63)\n\nProtein 2: MARK4 (Q96L34)\n Function: Serine/threonine-protein kinase (PubMed:14594945, PubMed:15009667, PubMed:23184942, PubMed:23666762). Phosphorylates the microtubule-associated protein MAPT/TAU (PubMed:14594945, PubMed:23666762). Als\n Location: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome\n\nEvidence: Systematic testing of PRR22 and MARK4 in a high-throughput assay did not identify this pair as interacting.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 410846, "gene_symbol_1": "PRR22", "gene_symbol_2": "MARK4", "detection_method": null}} +{"question_id": "PPIL1-0549", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: SRP14 (P37108)\n Function: Component of the signal recognition particle (SRP) complex, a ribonucleoprotein complex that mediates the cotranslational targeting of secretory and membrane proteins to the endoplasmic reticulum (ER)\n Location: Cytoplasm\n\nProtein 2: SMTN (P53814)\n Function: Structural protein of the cytoskeleton\n Location: Cytoplasm, cytoskeleton\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair SRP14-SMTN did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 209955, "gene_symbol_1": "SRP14", "gene_symbol_2": "SMTN", "detection_method": null}} +{"question_id": "PPIL1-0550", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: NGLY1 (Q96IV0)\n Function: Specifically deglycosylates the denatured form of N-linked glycoproteins in the cytoplasm and assists their proteasome-mediated degradation. Cleaves the beta-aspartyl-glucosamine (GlcNAc) of the glyca\n Location: Cytoplasm\n\nProtein 2: SLC25A23 (Q9BV35)\n Function: Electroneutral antiporter that mediates the transport of adenine nucleotides through the inner mitochondrial membrane. Originally identified as an ATP-magnesium/inorganic phosphate antiporter, it also\n Location: Mitochondrion inner membrane\n\nEvidence: Systematic testing of NGLY1 and SLC25A23 in a high-throughput assay did not identify this pair as interacting.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 461432, "gene_symbol_1": "NGLY1", "gene_symbol_2": "SLC25A23", "detection_method": null}} +{"question_id": "PPIL1-0551", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: DACH1 (Q9UI36)\n Function: Transcription factor that is involved in regulation of organogenesis. Seems to be a regulator of SIX1, SIX6 and probably SIX5. Corepression of precursor cell proliferation in myoblasts by SIX1 is swit\n Location: Nucleus\n\nProtein 2: AK6 (Q9Y3D8)\n Function: Broad-specificity nucleoside monophosphate (NMP) kinase that catalyzes the reversible transfer of the terminal phosphate group between nucleoside triphosphates and monophosphates. Also has ATPase acti\n Location: Cytoplasm\n\nEvidence: A large-scale binary interaction screen systematically tested DACH1 against AK6. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 498699, "gene_symbol_1": "DACH1", "gene_symbol_2": "AK6", "detection_method": null}} +{"question_id": "PPIL1-0552", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: C1QBP (Q07021)\n Function: Multifunctional and multicompartmental protein involved in inflammation and infection processes, ribosome biogenesis, protein synthesis in mitochondria, regulation of apoptosis, transcriptional regula\n Location: Mitochondrion matrix\n\nProtein 2: ISM2 (Q6H9L7)\n Location: Secreted\n\nEvidence: A large-scale binary interaction screen systematically tested C1QBP against ISM2. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 282194, "gene_symbol_1": "C1QBP", "gene_symbol_2": "ISM2", "detection_method": null}} +{"question_id": "PPIL1-0553", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: CALR (P27797)\n Function: Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with a\n Location: Endoplasmic reticulum lumen\n\nProtein 2: TMEM128 (Q5BJH2)\n Location: Membrane\n\nEvidence: Systematic testing of CALR and TMEM128 in a high-throughput assay did not identify this pair as interacting.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 192561, "gene_symbol_1": "CALR", "gene_symbol_2": "TMEM128", "detection_method": null}} +{"question_id": "PPIL1-0554", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: ZSCAN20 (P17040)\n Function: May be involved in transcriptional regulation\n Location: Nucleus\n\nProtein 2: TFAP4 (Q01664)\n Function: Transcription factor that activates both viral and cellular genes by binding to the symmetrical DNA sequence 5'-CAGCTG-3'\n Location: Nucleus\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair ZSCAN20-TFAP4 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 171039, "gene_symbol_1": "ZSCAN20", "gene_symbol_2": "TFAP4", "detection_method": null}} +{"question_id": "PPIL1-0555", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: ZNF623 (O75123)\n Function: May be involved in transcriptional regulation\n Location: Nucleus\n\nProtein 2: ISYNA1 (Q9NPH2)\n Function: Key enzyme in myo-inositol biosynthesis pathway that catalyzes the conversion of glucose 6-phosphate to 1-myo-inositol 1-phosphate in a NAD-dependent manner (PubMed:15024000, PubMed:23902760). Rate-li\n Location: Cytoplasm\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair ZNF623-ISYNA1 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 82986, "gene_symbol_1": "ZNF623", "gene_symbol_2": "ISYNA1", "detection_method": null}} +{"question_id": "PPIL1-0556", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: TLX2 (O43763)\n Function: Transcription activator that binds DNA elements with the consensus sequence 5'-CGGTAATTGG-3'. Binds DNA via its homeobox. Required for normal cell death of enteric neurons in the gastrointestinal trac\n Location: Nucleus\n\nProtein 2: DMTN (Q08495)\n Function: Membrane-cytoskeleton-associated protein with F-actin-binding activity that induces F-actin bundles formation and stabilization. Its F-actin-bundling activity is reversibly regulated upon its phosphor\n Location: Cytoplasm\n\nEvidence: Systematic testing of TLX2 and DMTN in a high-throughput assay did not identify this pair as interacting.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 67927, "gene_symbol_1": "TLX2", "gene_symbol_2": "DMTN", "detection_method": null}} +{"question_id": "PPIL1-0557", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: ZNF664 (Q8N3J9)\n Function: May be involved in transcriptional regulation\n Location: Nucleus\n\nProtein 2: TMIE (Q8NEW7)\n Function: Auxiliary subunit of the mechanotransducer (MET) non-specific cation channel complex located at the tips of stereocilia of cochlear hair cells and that mediates sensory transduction in the auditory sy\n Location: Membrane\n\nEvidence: A large-scale binary interaction screen systematically tested ZNF664 against TMIE. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 416106, "gene_symbol_1": "ZNF664", "gene_symbol_2": "TMIE", "detection_method": null}} +{"question_id": "PPIL1-0558", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: RRH (O14718)\n Function: May play a role in rpe physiology either by detecting light directly or by monitoring the concentration of retinoids or other photoreceptor-derived compounds\n Location: Membrane\n\nProtein 2: UBALD1 (Q8TB05)\n\nEvidence: A large-scale binary interaction screen systematically tested RRH against UBALD1. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 39385, "gene_symbol_1": "RRH", "gene_symbol_2": "UBALD1", "detection_method": null}} +{"question_id": "PPIL1-0559", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: KDELR1 (P24390)\n Function: Receptor for the C-terminal sequence motif K-D-E-L that is present on endoplasmic reticulum resident proteins and that mediates their recycling from the Golgi back to the endoplasmic reticulum\n Location: Golgi apparatus membrane\n\nProtein 2: EMC2 (Q15006)\n Function: Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins (PubMed:29242\n Location: Endoplasmic reticulum membrane\n\nEvidence: Systematic testing of KDELR1 and EMC2 in a high-throughput assay did not identify this pair as interacting.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 186536, "gene_symbol_1": "KDELR1", "gene_symbol_2": "EMC2", "detection_method": null}} +{"question_id": "PPIL1-0560", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: SMIM1 (B2RUZ4)\n Function: Regulator of red blood cell formation\n Location: Cell membrane\n\nProtein 2: CARD14 (Q9BXL6)\n Function: Acts as a scaffolding protein that can activate the inflammatory transcription factor NF-kappa-B and p38/JNK MAP kinase signaling pathways. Forms a signaling complex with BCL10 and MALT1, and activate\n Location: Cytoplasm\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair SMIM1-CARD14 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 19863, "gene_symbol_1": "SMIM1", "gene_symbol_2": "CARD14", "detection_method": null}} +{"question_id": "PPIL1-0561", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: SV2B (Q7L1I2)\n Function: Probably plays a role in the control of regulated secretion in neural and endocrine cells\n Location: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane\n\nProtein 2: EZHIP (Q86X51)\n Function: Inhibits PRC2/EED-EZH1 and PRC2/EED-EZH2 complex function by inhibiting EZH1/EZH2 methyltransferase activity, thereby causing down-regulation of histone H3 trimethylation on 'Lys-27' (H3K27me3) (PubMe\n Location: Nucleus\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair SV2B-EZHIP did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 384991, "gene_symbol_1": "SV2B", "gene_symbol_2": "EZHIP", "detection_method": null}} +{"question_id": "PPIL1-0562", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: GPR152 (Q8TDT2)\n Function: Orphan receptor\n Location: Cell membrane\n\nProtein 2: CASS4 (Q9NQ75)\n Function: Docking protein that plays a role in tyrosine kinase-based signaling related to cell adhesion and cell spreading. Regulates PTK2/FAK1 activity, focal adhesion integrity, and cell spreading\n Location: Cytoplasm, cytoskeleton\n\nEvidence: A large-scale binary interaction screen systematically tested GPR152 against CASS4. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 436631, "gene_symbol_1": "GPR152", "gene_symbol_2": "CASS4", "detection_method": null}} +{"question_id": "PPIL1-0563", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: IFNA14 (P01570)\n Function: Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase\n Location: Secreted\n\nProtein 2: SMIM3 (Q9BZL3)\n Location: Membrane\n\nEvidence: A large-scale binary interaction screen systematically tested IFNA14 against SMIM3. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 122958, "gene_symbol_1": "IFNA14", "gene_symbol_2": "SMIM3", "detection_method": null}} +{"question_id": "PPIL1-0564", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: TSHZ3 (Q63HK5)\n Function: Transcriptional regulator involved in developmental processes. Functions in association with APBB1, SET and HDAC factors as a transcriptional repressor, that inhibits the expression of CASP4. TSHZ3-me\n Location: Nucleus\n\nProtein 2: CNNM1 (Q9NRU3)\n Function: Probable metal transporter\n Location: Cell membrane\n\nEvidence: Systematic testing of TSHZ3 and CNNM1 in a high-throughput assay did not identify this pair as interacting.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 358193, "gene_symbol_1": "TSHZ3", "gene_symbol_2": "CNNM1", "detection_method": null}} +{"question_id": "PPIL1-0565", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: TRIM59 (Q8IWR1)\n Function: E3 ubiquitin ligase involved in different processes such as development and immune response (PubMed:22588174, PubMed:30231667). Serves as a negative regulator for innate immune signaling pathways by s\n Location: Endoplasmic reticulum membrane\n\nProtein 2: SLC26A1 (Q9H2B4)\n Function: Sodium-independent sulfate anion transporter (PubMed:12713736, PubMed:27125215). Can transport other anions including bicarbonate, thiosulfate and oxalate by mediating sulfate-thiosulfate, sulfate-hyd\n Location: Cell membrane\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair TRIM59-SLC26A1 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 405825, "gene_symbol_1": "TRIM59", "gene_symbol_2": "SLC26A1", "detection_method": null}} +{"question_id": "PPIL1-0566", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: MT1X (P80297)\n Function: Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids. May be involved in FA\n\nProtein 2: TRAM1 (Q15629)\n Function: Involved in the translocation of nascent protein chains into or through the endoplasmic reticulum (ER) membrane by facilitating the proper chain positioning at the SEC61 channel (PubMed:12475939, PubM\n Location: Endoplasmic reticulum membrane\n\nEvidence: A large-scale binary interaction screen systematically tested MT1X against TRAM1. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 268429, "gene_symbol_1": "MT1X", "gene_symbol_2": "TRAM1", "detection_method": null}} +{"question_id": "PPIL1-0567", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: SEPTIN12 (Q8IYM1)\n Function: Filament-forming cytoskeletal GTPase (By similarity). Involved in spermatogenesis. Involved in the morphogenesis of sperm heads and the elongation of sperm tails probably implicating the association w\n Location: Cytoplasm\n\nProtein 2: LRRC25 (Q8N386)\n Function: Plays a role in the inhibition of RLR-mediated type I interferon signaling pathway by targeting RIGI for autophagic degradation. Interacts specifically with ISG15-associated RIGI to promote interactio\n Location: Membrane\n\nEvidence: A large-scale binary interaction screen systematically tested SEPTIN12 against LRRC25. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 409435, "gene_symbol_1": "SEPTIN12", "gene_symbol_2": "LRRC25", "detection_method": null}} +{"question_id": "PPIL1-0568", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: NKD1 (Q969G9)\n Function: Cell autonomous antagonist of the canonical Wnt signaling pathway. May activate a second Wnt signaling pathway that controls planar cell polarity\n Location: Cell membrane\n\nProtein 2: YIPF3 (Q9GZM5)\n Function: Involved in the maintenance of the Golgi structure. May play a role in hematopoiesis\n Location: Cell membrane\n\nEvidence: A large-scale binary interaction screen systematically tested NKD1 against YIPF3. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 449407, "gene_symbol_1": "NKD1", "gene_symbol_2": "YIPF3", "detection_method": null}} +{"question_id": "PPIL1-0569", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: ARHGEF1 (Q92888)\n Function: Seems to play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) subunits (PubMed:9641915, PubMed:9641916). Acts as a GTPase-activating protein\n Location: Cytoplasm\n\nProtein 2: TMX1 (Q9H3N1)\n Function: Thiredoxin domain-containing protein that participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyze dithiol-disulfide exchange r\n Location: Endoplasmic reticulum membrane\n\nEvidence: A large-scale binary interaction screen systematically tested ARHGEF1 against TMX1. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 447709, "gene_symbol_1": "ARHGEF1", "gene_symbol_2": "TMX1", "detection_method": null}} +{"question_id": "PPIL1-0570", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: EBP (Q15125)\n Function: Isomerase that catalyzes the conversion of Delta(8)-sterols to their corresponding Delta(7)-isomers a catalytic step in the postlanosterol biosynthesis of cholesterol\n Location: Endoplasmic reticulum membrane\n\nProtein 2: AASDHPPT (Q9NRN7)\n Function: Catalyzes the post-translational modification of target proteins by phosphopantetheine. Can transfer the 4'-phosphopantetheine moiety from coenzyme A, regardless of whether the CoA is presented in the\n Location: Cytoplasm, cytosol\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair EBP-AASDHPPT did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 314298, "gene_symbol_1": "EBP", "gene_symbol_2": "AASDHPPT", "detection_method": null}} +{"question_id": "PPIL1-0571", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: KIF1B (O60333)\n Function: Has a plus-end-directed microtubule motor activity and functions as a motor for transport of vesicles and organelles along microtubules\n Location: Cytoplasm, cytoskeleton\n\nProtein 2: CCDC197 (Q8NCU1)\n\nEvidence: A large-scale binary interaction screen systematically tested KIF1B against CCDC197. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 73152, "gene_symbol_1": "KIF1B", "gene_symbol_2": "CCDC197", "detection_method": null}} +{"question_id": "PPIL1-0572", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: EPHB6 (A0A087WZL4)\n\nProtein 2: TMEM9B (Q9NQ34)\n Function: Enhances production of pro-inflammatory cytokines induced by TNF, IL1B, and TLR ligands. Has a role in TNF activation of both the NF-kappaB and MAPK pathways\n Location: Lysosome membrane\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair EPHB6-TMEM9B did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 1711, "gene_symbol_1": "EPHB6", "gene_symbol_2": "TMEM9B", "detection_method": null}} +{"question_id": "PPIL1-0573", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: NUAK2 (Q9H093)\n Function: Stress-activated kinase involved in tolerance to glucose starvation. Induces cell-cell detachment by increasing F-actin conversion to G-actin. Expression is induced by CD95 or TNF, via NF-kappa-B. Pro\n\nProtein 2: FADS3 (Q9Y5Q0)\n Function: Mammals have different sphingoid bases that differ in their length and/or pattern of desaturation and hydroxyl groups. The predominant sphingoid base that comprises mammalian ceramides is sphing-4-eni\n Location: Endoplasmic reticulum membrane\n\nEvidence: Systematic testing of NUAK2 and FADS3 in a high-throughput assay did not identify this pair as interacting.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 484408, "gene_symbol_1": "NUAK2", "gene_symbol_2": "FADS3", "detection_method": null}} +{"question_id": "PPIL1-0574", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: CTAG2 (O75638)\n\nProtein 2: TMEM147 (Q9BVK8)\n Function: Component of the multi-pass translocon (MPT) complex that mediates insertion of multi-pass membrane proteins into the lipid bilayer of membranes (PubMed:32820719, PubMed:36261522). The MPT complex tak\n Location: Endoplasmic reticulum membrane\n\nEvidence: Systematic testing of CTAG2 and TMEM147 in a high-throughput assay did not identify this pair as interacting.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 91073, "gene_symbol_1": "CTAG2", "gene_symbol_2": "TMEM147", "detection_method": null}} +{"question_id": "PPIL1-0575", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: TBP (P20226)\n Function: The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription (PubMed:33795473). TFIID recognizes and binds promoters with or \n Location: Nucleus\n\nProtein 2: RBM4B (Q9BQ04)\n Function: Required for the translational activation of PER1 mRNA in response to circadian clock. Binds directly to the 3'-UTR of the PER1 mRNA (By similarity)\n Location: Nucleus\n\nEvidence: A large-scale binary interaction screen systematically tested TBP against RBM4B. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 177120, "gene_symbol_1": "TBP", "gene_symbol_2": "RBM4B", "detection_method": null}} +{"question_id": "PPIL1-0576", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: SCD (O00767)\n Function: Stearoyl-CoA desaturase that utilizes O(2) and electrons from reduced cytochrome b5 to introduce the first double bond into saturated fatty acyl-CoA substrates (PubMed:15907797, PubMed:18765284). Cata\n Location: Endoplasmic reticulum membrane\n\nProtein 2: MICOS10 (Q5TGZ0)\n Function: Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation \n Location: Mitochondrion inner membrane\n\nEvidence: A large-scale binary interaction screen systematically tested SCD against MICOS10. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 34326, "gene_symbol_1": "SCD", "gene_symbol_2": "MICOS10", "detection_method": null}} +{"question_id": "PPIL1-0577", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: HCLS1 (P14317)\n Function: Substrate of the antigen receptor-coupled tyrosine kinase. Plays a role in antigen receptor signaling for both clonal expansion and deletion in lymphoid cells. May also be involved in the regulation o\n Location: Membrane\n\nProtein 2: SYT6 (Q5T7P8)\n Function: May be involved in Ca(2+)-dependent exocytosis of secretory vesicles through Ca(2+) and phospholipid binding to the C2 domain or may serve as Ca(2+) sensors in the process of vesicular trafficking and\n Location: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair HCLS1-SYT6 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 162708, "gene_symbol_1": "HCLS1", "gene_symbol_2": "SYT6", "detection_method": null}} +{"question_id": "PPIL1-0578", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: CLDN8 (P56748)\n Function: Can associate with other claudins to regulate tight junction structural and functional strand dynamics (By similarity). May coassemble with CLDN4 into tight junction strands containing anion-selective\n Location: Cell junction, tight junction\n\nProtein 2: KLK4 (Q9Y5K2)\n Function: Has a major role in enamel formation (PubMed:15235027). Required during the maturation stage of tooth development for clearance of enamel proteins and normal structural patterning of the crystalline m\n Location: Secreted\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair CLDN8-KLK4 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 248343, "gene_symbol_1": "CLDN8", "gene_symbol_2": "KLK4", "detection_method": null}} +{"question_id": "PPIL1-0579", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: PTPN21 (Q16825)\n Location: Cytoplasm, cytoskeleton\n\nProtein 2: RHCG (Q9UBD6)\n Function: Ammonium transporter involved in the maintenance of acid-base homeostasis. Transports ammonium and its related derivative methylammonium across the plasma membrane of epithelial cells likely contribut\n Location: Cell membrane\n\nEvidence: A large-scale binary interaction screen systematically tested PTPN21 against RHCG. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 327024, "gene_symbol_1": "PTPN21", "gene_symbol_2": "RHCG", "detection_method": null}} +{"question_id": "PPIL1-0580", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: ANKK1 (Q8NFD2)\n\nProtein 2: TIMM13 (Q9Y5L4)\n Function: Mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of b\n Location: Mitochondrion inner membrane\n\nEvidence: A large-scale binary interaction screen systematically tested ANKK1 against TIMM13. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 429102, "gene_symbol_1": "ANKK1", "gene_symbol_2": "TIMM13", "detection_method": null}} +{"question_id": "PPIL1-0581", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: SPDYC (Q5MJ68)\n Function: Promotes progression through the cell cycle via binding and activation of CDK1 and CDK2. Involved in the spindle-assembly checkpoint. Required for recruitment of MAD2L1, BUBR1 and BUB1 to kinetochores\n Location: Cytoplasm\n\nProtein 2: STRAP (Q9Y3F4)\n Function: The SMN complex catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, and thereby plays an important role in the splicing of cellular pre-mRNAs. \n Location: Cytoplasm\n\nEvidence: Systematic testing of SPDYC and STRAP in a high-throughput assay did not identify this pair as interacting.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 347006, "gene_symbol_1": "SPDYC", "gene_symbol_2": "STRAP", "detection_method": null}} +{"question_id": "PPIL1-0582", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: CCDC78 (A2IDD5)\n Function: Component of the deuterosome, a structure that promotes de novo centriole amplification in multiciliated cells that can generate more than 100 centrioles. Deuterosome-mediated centriole amplification \n Location: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole\n\nProtein 2: CNPY3 (Q9BT09)\n Function: Toll-like receptor (TLR)-specific co-chaperone for HSP90B1. Required for proper TLR folding, except that of TLR3, and hence controls TLR exit from the endoplasmic reticulum. Consequently, required for\n Location: Endoplasmic reticulum\n\nEvidence: A large-scale binary interaction screen systematically tested CCDC78 against CNPY3. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 9166, "gene_symbol_1": "CCDC78", "gene_symbol_2": "CNPY3", "detection_method": null}} +{"question_id": "PPIL1-0583", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: RNF213 (Q63HN8)\n Function: Atypical E3 ubiquitin ligase that can catalyze ubiquitination of both proteins and lipids, and which is involved in various processes, such as lipid metabolism, angiogenesis and cell-autonomous immuni\n Location: Cytoplasm, cytosol\n\nProtein 2: MSI2 (Q96DH6)\n Function: RNA binding protein that regulates the expression of target mRNAs at the translation level. May play a role in the proliferation and maintenance of stem cells in the central nervous system (By similar\n Location: Cytoplasm\n\nEvidence: A large-scale binary interaction screen systematically tested RNF213 against MSI2. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 358387, "gene_symbol_1": "RNF213", "gene_symbol_2": "MSI2", "detection_method": null}} +{"question_id": "PPIL1-0584", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: DRAP1 (Q14919)\n Function: The association of the DR1/DRAP1 heterodimer with TBP results in a functional repression of both activated and basal transcription of class II genes. This interaction precludes the formation of a tran\n Location: Nucleus\n\nProtein 2: GPN3 (Q9UHW5)\n Function: Small GTPase involved in the correct assembly of RNA polymerase II (RNAPII) complex, ensuring proper nuclear import of RNAPII\n Location: Cytoplasm\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair DRAP1-GPN3 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 310558, "gene_symbol_1": "DRAP1", "gene_symbol_2": "GPN3", "detection_method": null}} +{"question_id": "PPIL1-0585", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: FTH1 (P02794)\n Function: Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity (PubMed:9003196). Iron is taken up in the ferrous form and deposited as ferric hyd\n Location: Cytoplasm\n\nProtein 2: SPSB3 (Q6PJ21)\n Function: Substrate-recognition component of a cullin-5-RING E3 ubiquitin-protein ligase complex (ECS complex, also named CRL5 complex), which mediates the ubiquitination and subsequent proteasomal degradation \n Location: Nucleus\n\nEvidence: Systematic testing of FTH1 and SPSB3 in a high-throughput assay did not identify this pair as interacting.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 126560, "gene_symbol_1": "FTH1", "gene_symbol_2": "SPSB3", "detection_method": null}} +{"question_id": "PPIL1-0586", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: DEFB124 (Q8NES8)\n Function: Has antibacterial activity\n Location: Secreted\n\nProtein 2: DAPP1 (Q9UN19)\n Function: May act as a B-cell-associated adapter that regulates B-cell antigen receptor (BCR)-signaling downstream of PI3K\n Location: Cytoplasm\n\nEvidence: A large-scale binary interaction screen systematically tested DEFB124 against DAPP1. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 428397, "gene_symbol_1": "DEFB124", "gene_symbol_2": "DAPP1", "detection_method": null}} +{"question_id": "PPIL1-0587", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: LSM11 (P83369)\n Function: Component of the U7 snRNP complex that is involved in the histone 3'-end pre-mRNA processing (PubMed:11574479, PubMed:16914750, PubMed:33230297). Increases U7 snRNA levels but not histone 3'-end pre-m\n Location: Nucleus\n\nProtein 2: CUEDC1 (Q9NWM3)\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair LSM11-CUEDC1 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 269656, "gene_symbol_1": "LSM11", "gene_symbol_2": "CUEDC1", "detection_method": null}} +{"question_id": "PPIL1-0588", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: CMC4 (P56277)\n Location: Mitochondrion\n\nProtein 2: NXT2 (Q9NPJ8)\n Function: Regulator of protein export for NES-containing proteins. Also plays a role in mRNA nuclear export\n Location: Nucleus\n\nEvidence: A large-scale binary interaction screen systematically tested CMC4 against NXT2. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 246845, "gene_symbol_1": "CMC4", "gene_symbol_2": "NXT2", "detection_method": null}} +{"question_id": "PPIL1-0589", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: NEFM (P07197)\n Function: Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal interm\n Location: Cytoplasm, cytoskeleton\n\nProtein 2: GRHL2 (Q6ISB3)\n Function: Transcription factor playing an important role in primary neurulation and in epithelial development (PubMed:25152456, PubMed:29309642). Binds directly to the consensus DNA sequence 5'-AACCGGTT-3' acti\n Location: Nucleus\n\nEvidence: Systematic testing of NEFM and GRHL2 in a high-throughput assay did not identify this pair as interacting.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 136493, "gene_symbol_1": "NEFM", "gene_symbol_2": "GRHL2", "detection_method": null}} +{"question_id": "PPIL1-0590", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: NAIF1 (Q69YI7)\n Function: Induces apoptosis\n Location: Nucleus\n\nProtein 2: APLF (Q8IW19)\n Function: Histone chaperone involved in single-strand and double-strand DNA break repair (PubMed:17353262, PubMed:17396150, PubMed:21211721, PubMed:21211722, PubMed:29905837, PubMed:30104678). Recruited to site\n Location: Nucleus\n\nEvidence: Systematic testing of NAIF1 and APLF in a high-throughput assay did not identify this pair as interacting.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 360746, "gene_symbol_1": "NAIF1", "gene_symbol_2": "APLF", "detection_method": null}} +{"question_id": "PPIL1-0591", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: SLC4A1 (P02730)\n Function: Functions both as a transporter that mediates electroneutral anion exchange across the cell membrane and as a structural protein (PubMed:10926824, PubMed:14734552, PubMed:1538405, PubMed:16227998, Pub\n Location: Cell membrane\n\nProtein 2: PNPLA5 (Q7Z6Z6)\n Function: Has abundant triacylglycerol lipase activity\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair SLC4A1-PNPLA5 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 125502, "gene_symbol_1": "SLC4A1", "gene_symbol_2": "PNPLA5", "detection_method": null}} +{"question_id": "PPIL1-0592", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: KRT39 (Q6A163)\n Function: May play a role in late hair differentiation\n\nProtein 2: OR52L1 (Q8NGH7)\n Function: Odorant receptor\n Location: Cell membrane\n\nEvidence: A large-scale binary interaction screen systematically tested KRT39 against OR52L1. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 361188, "gene_symbol_1": "KRT39", "gene_symbol_2": "OR52L1", "detection_method": null}} +{"question_id": "PPIL1-0593", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: SULT1A3 (P0DMM9)\n Function: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of phenolic monoamines (neurotransmitters such as dopamine, (R)-adrenaline/e\n Location: Cytoplasm\n\nProtein 2: RBM23 (Q86U06)\n Function: RNA-binding protein that acts both as a transcription coactivator and pre-mRNA splicing factor (PubMed:15694343). Regulates steroid hormone receptor-mediated transcription, independently of the pre-mR\n Location: Nucleus\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair SULT1A3-RBM23 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 150438, "gene_symbol_1": "SULT1A3", "gene_symbol_2": "RBM23", "detection_method": null}} +{"question_id": "PPIL1-0594", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: ZNF593 (O00488)\n Function: Involved in pre-60S ribosomal particles maturation by promoting the nuclear export of the 60S ribosome (PubMed:32669547). Negatively modulates the DNA binding activity of Oct-2 and therefore its trans\n Location: Nucleus, nucleolus\n\nProtein 2: R3HDM2 (Q9Y2K5)\n Location: Nucleus\n\nEvidence: A large-scale binary interaction screen systematically tested ZNF593 against R3HDM2. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 31467, "gene_symbol_1": "ZNF593", "gene_symbol_2": "R3HDM2", "detection_method": null}} +{"question_id": "PPIL1-0595", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: RIMOC1 (A6NDU8)\n Function: Plays an important role in the removal of damaged mitochondria via mitophagy by controlling the stability and localization of RAB7A. Required for the recruitment of RAB7A and ATG9A vesicles to damaged\n Location: Cytoplasm, cytosol\n\nProtein 2: ZNF775 (Q96BV0)\n Function: May be involved in transcriptional regulation\n Location: Nucleus\n\nEvidence: In a genome-wide yeast two-hybrid (Y2H) screen testing all pairwise combinations of human ORFs, the pair RIMOC1-ZNF775 did not produce a positive signal in any replicate.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 12418, "gene_symbol_1": "RIMOC1", "gene_symbol_2": "ZNF775", "detection_method": null}} +{"question_id": "PPIL1-0596", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: PSG1 (P11464)\n Location: Secreted\n\nProtein 2: IL21 (Q9HBE4)\n Function: Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells (By similarity). Implicated in the gene\n Location: Secreted\n\nEvidence: Systematic testing of PSG1 and IL21 in a high-throughput assay did not identify this pair as interacting.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 156642, "gene_symbol_1": "PSG1", "gene_symbol_2": "IL21", "detection_method": null}} +{"question_id": "PPIL1-0597", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: SPTA1 (P02549)\n Function: Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocy\n Location: Cytoplasm, cytoskeleton\n\nProtein 2: TRPM5 (Q9NZQ8)\n Function: Monovalent cation-selective ion channel activated by intracellular Ca(2+) in a voltage- and temperature-dependent manner (PubMed:14634208). Mediates the transport of Na(+), K(+) and Cs(+) ions equally\n Location: Cell membrane\n\nEvidence: A large-scale binary interaction screen systematically tested SPTA1 against TRPM5. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 124386, "gene_symbol_1": "SPTA1", "gene_symbol_2": "TRPM5", "detection_method": null}} +{"question_id": "PPIL1-0598", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: BATF (Q16520)\n Function: AP-1 family transcription factor that controls the differentiation of lineage-specific cells in the immune system: specifically mediates the differentiation of T-helper 17 cells (Th17), follicular T-h\n Location: Nucleus\n\nProtein 2: ERICH1 (Q86X53)\n\nEvidence: Systematic testing of BATF and ERICH1 in a high-throughput assay did not identify this pair as interacting.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 323153, "gene_symbol_1": "BATF", "gene_symbol_2": "ERICH1", "detection_method": null}} +{"question_id": "PPIL1-0599", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: PHKB (Q93100)\n Function: Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. The beta chain acts as a regulatory unit and modulates the activity of the holoenzyme in res\n Location: Cell membrane\n\nProtein 2: PRDM16 (Q9HAZ2)\n Function: Transcription regulator that acts both as a histone methyltransferase or chromatin adapter, depending on the context (PubMed:12816872). In the cytoplasm, acts as a histone methyltransferase, which cat\n Location: Nucleus\n\nEvidence: A large-scale binary interaction screen systematically tested PHKB against PRDM16. The pair was not detected among the positive interactions.", "gold_answer": "B", "gold_category": "systematic_screen", "metadata": {"source_db": "huri", "confidence_tier": "gold", "result_id": 449064, "gene_symbol_1": "PHKB", "gene_symbol_2": "PRDM16", "detection_method": null}} +{"question_id": "PPIL1-0600", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: COLEC10 (Q9Y6Z7)\n Function: Lectin that binds to various sugars: galactose > mannose = fucose > N-acetylglucosamine > N-acetylgalactosamine (PubMed:10224141). Acts as a chemoattractant, probably involved in the regulation of cel\n Location: Secreted\n\nProtein 2: CENPW (Q5EE01)\n Function: Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation (By similarity). The\n Location: Nucleus\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that COLEC10 and CENPW co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1150398, "gene_symbol_1": "COLEC10", "gene_symbol_2": "CENPW", "detection_method": null}} +{"question_id": "PPIL1-0601", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: VPS28 (Q9UK41)\n Function: Component of the ESCRT-I complex, a regulator of vesicular trafficking process\n Location: Cell membrane\n\nProtein 2: F13B (P05160)\n Function: The B chain of factor XIII is not catalytically active, but is thought to stabilize the A subunits and regulate the rate of transglutaminase formation by thrombin\n Location: Secreted\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that VPS28 and F13B do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1623949, "gene_symbol_1": "VPS28", "gene_symbol_2": "F13B", "detection_method": null}} +{"question_id": "PPIL1-0602", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: TINF2 (Q9BSI4)\n Function: Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomera\n Location: Nucleus\n\nProtein 2: HDAC1 (Q13547)\n Function: Histone deacetylase that catalyzes the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) (PubMed:16762839, PubMed:17704056, PubMed:28497810). Histone d\n Location: Nucleus\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that TINF2 and HDAC1 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 785495, "gene_symbol_1": "TINF2", "gene_symbol_2": "HDAC1", "detection_method": null}} +{"question_id": "PPIL1-0603", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: CLOCK (O15516)\n Function: Transcriptional activator which forms a core component of the circadian clock. The circadian clock, an internal time-keeping system, regulates various physiological processes through the generation of\n Location: Nucleus\n\nProtein 2: YEATS4 (O95619)\n Function: Chromatin reader component of the NuA4 histone acetyltransferase (HAT) complex, a complex involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 a\n Location: Nucleus\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that CLOCK and YEATS4 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1401384, "gene_symbol_1": "CLOCK", "gene_symbol_2": "YEATS4", "detection_method": null}} +{"question_id": "PPIL1-0604", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: MCM7 (P33993)\n Function: Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core compon\n Location: Nucleus\n\nProtein 2: CCNY (Q8ND76)\n Function: Positive regulatory subunit of the cyclin-dependent kinases CDK14/PFTK1 and CDK16. Acts as a cell-cycle regulator of Wnt signaling pathway during G2/M phase by recruiting CDK14/PFTK1 to the plasma mem\n Location: Cell membrane\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that MCM7 and CCNY do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 925905, "gene_symbol_1": "MCM7", "gene_symbol_2": "CCNY", "detection_method": null}} +{"question_id": "PPIL1-0605", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: MRPS18B (Q9Y676)\n Location: Mitochondrion\n\nProtein 2: CPSF3 (Q9UKF6)\n Function: Component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) \n Location: Nucleus\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that MRPS18B and CPSF3 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1199959, "gene_symbol_1": "MRPS18B", "gene_symbol_2": "CPSF3", "detection_method": null}} +{"question_id": "PPIL1-0606", "task": "ppi-l1", "split": "fewshot", "difficulty": "hard", "context_text": "Protein 1: H2BC11 (P06899)\n Function: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central r\n Location: Nucleus\n\nProtein 2: PPP3CC (P48454)\n Function: Calcium-dependent, calmodulin-stimulated protein phosphatase which plays an essential role in the transduction of intracellular Ca(2+)-mediated signals. Dephosphorylates and activates transcription fa\n Location: Mitochondrion\n\nEvidence: Analysis of protein complex data indicates H2BC11 and PPP3CC are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 849904, "gene_symbol_1": "H2BC11", "gene_symbol_2": "PPP3CC", "detection_method": null}} +{"question_id": "PPIL1-0607", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: DNAAF2 (Q9NVR5)\n Function: Required for cytoplasmic pre-assembly of axonemal dyneins, thereby playing a central role in motility in cilia and flagella. Involved in pre-assembly of dynein arm complexes in the cytoplasm before in\n Location: Cytoplasm\n\nProtein 2: NSMCE1 (Q8WV22)\n Function: RING-type zinc finger-containing E3 ubiquitin ligase that assembles with melanoma antigen protein (MAGE) to catalyze the direct transfer of ubiquitin from E2 ubiquitin-conjugating enzyme to a specific\n Location: Nucleus\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that DNAAF2 and NSMCE1 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 623607, "gene_symbol_1": "DNAAF2", "gene_symbol_2": "NSMCE1", "detection_method": null}} +{"question_id": "PPIL1-0608", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: PRIM1 (P49642)\n Function: Catalytic subunit of the DNA primase complex and component of the DNA polymerase alpha complex (also known as the alpha DNA polymerase-primase complex - primosome/replisome) which play an essential ro\n\nProtein 2: GPAA1 (O43292)\n Function: Component of the glycosylphosphatidylinositol-anchor (GPI-anchor) transamidase (GPI-T) complex that catalyzes the formation of the linkage between a proprotein and a GPI-anchor and participates in GPI\n Location: Endoplasmic reticulum membrane\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that PRIM1 and GPAA1 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1513876, "gene_symbol_1": "PRIM1", "gene_symbol_2": "GPAA1", "detection_method": null}} +{"question_id": "PPIL1-0609", "task": "ppi-l1", "split": "fewshot", "difficulty": "hard", "context_text": "Protein 1: STAT3 (P40763)\n Function: Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors (PubMed:10688651, PubMed:12359225, PubMed:12873986, PubMed:15194\n Location: Cytoplasm\n\nProtein 2: GABRA6 (Q16445)\n Function: Alpha subunit of the heteropentameric ligand-gated chloride channel gated by gamma-aminobutyric acid (GABA), a major inhibitory neurotransmitter in the brain (PubMed:8632757). GABA-gated chloride chan\n Location: Postsynaptic cell membrane\n\nEvidence: Analysis of protein complex data indicates STAT3 and GABRA6 are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 898054, "gene_symbol_1": "STAT3", "gene_symbol_2": "GABRA6", "detection_method": null}} +{"question_id": "PPIL1-0610", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: TRAPPC13 (A5PLN9)\n\nProtein 2: SMARCA4 (P51532)\n Function: ATPase involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that c\n Location: Nucleus\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that TRAPPC13 and SMARCA4 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 848673, "gene_symbol_1": "TRAPPC13", "gene_symbol_2": "SMARCA4", "detection_method": null}} +{"question_id": "PPIL1-0611", "task": "ppi-l1", "split": "fewshot", "difficulty": "hard", "context_text": "Protein 1: VPS28 (Q9UK41)\n Function: Component of the ESCRT-I complex, a regulator of vesicular trafficking process\n Location: Cell membrane\n\nProtein 2: HSP90AA1 (P07900)\n Function: Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoe\n Location: Nucleus\n\nEvidence: Analysis of protein complex data indicates VPS28 and HSP90AA1 are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 549120, "gene_symbol_1": "VPS28", "gene_symbol_2": "HSP90AA1", "detection_method": null}} +{"question_id": "PPIL1-0612", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: NXF2 (Q9GZY0)\n Function: Involved in the export of mRNA from the nucleus to the cytoplasm\n Location: Nucleus, nucleoplasm\n\nProtein 2: CSTF2T (Q9H0L4)\n Function: May play a significant role in AAUAAA-independent mRNA polyadenylation in germ cells. Directly involved in the binding to pre-mRNAs (By similarity)\n Location: Nucleus\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that NXF2 and CSTF2T do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1330219, "gene_symbol_1": "NXF2", "gene_symbol_2": "CSTF2T", "detection_method": null}} +{"question_id": "PPIL1-0613", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: CDK11B (P21127)\n Function: Cyclin-dependent protein kinase that acts as a regulator of transcription and pre-mRNA splicing (PubMed:12501247, PubMed:18216018, PubMed:32367068, PubMed:36104565). Acts as a key regulator of pre-mRN\n Location: Nucleus\n\nProtein 2: ETFB (P38117)\n Function: Heterodimeric electron transfer flavoprotein that accepts electrons from several mitochondrial dehydrogenases, including acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase (PubMed:15159\n Location: Mitochondrion matrix\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that CDK11B and ETFB co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 871232, "gene_symbol_1": "CDK11B", "gene_symbol_2": "ETFB", "detection_method": null}} +{"question_id": "PPIL1-0614", "task": "ppi-l1", "split": "fewshot", "difficulty": "hard", "context_text": "Protein 1: RXRB (P28702)\n Function: Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression \n Location: Nucleus\n\nProtein 2: PDCD10 (Q9BUL8)\n Function: Promotes cell proliferation. Modulates apoptotic pathways. Increases mitogen-activated protein kinase activity and STK26 activity (PubMed:27807006). Important for cell migration, and for normal struct\n Location: Cytoplasm\n\nEvidence: Analysis of protein complex data indicates RXRB and PDCD10 are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 707188, "gene_symbol_1": "RXRB", "gene_symbol_2": "PDCD10", "detection_method": null}} +{"question_id": "PPIL1-0615", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: PYCARD (Q9ULZ3)\n Function: Functions as a key mediator in apoptosis and inflammation (PubMed:11103777, PubMed:12646168, PubMed:15030775, PubMed:17349957, PubMed:17599095, PubMed:19158675, PubMed:19158676, PubMed:19234215, PubMe\n Location: Cytoplasm\n\nProtein 2: GABRA2 (P47869)\n Function: Alpha subunit of the heteropentameric ligand-gated chloride channel gated by gamma-aminobutyric acid (GABA), a major inhibitory neurotransmitter in the brain (PubMed:10449790, PubMed:29961870, PubMed:\n Location: Postsynaptic cell membrane\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that PYCARD and GABRA2 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1058845, "gene_symbol_1": "PYCARD", "gene_symbol_2": "GABRA2", "detection_method": null}} +{"question_id": "PPIL1-0616", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: COL1A1 (P02452)\n Function: Type I collagen is a member of group I collagen (fibrillar forming collagen)\n Location: Secreted, extracellular space, extracellular matrix\n\nProtein 2: ABRAXAS1 (Q6UWZ7)\n Function: Involved in DNA damage response and double-strand break (DSB) repair. Component of the BRCA1-A complex, acting as a central scaffold protein that assembles the various components of the complex and me\n Location: Nucleus\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that COL1A1 and ABRAXAS1 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 623929, "gene_symbol_1": "COL1A1", "gene_symbol_2": "ABRAXAS1", "detection_method": null}} +{"question_id": "PPIL1-0617", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: TFDP1 (Q14186)\n Function: Can stimulate E2F-dependent transcription. Binds DNA cooperatively with E2F family members through the E2 recognition site, 5'-TTTC[CG]CGC-3', found in the promoter region of a number of genes whose p\n Location: Nucleus\n\nProtein 2: TADA2B (Q86TJ2)\n Function: Coactivates PAX5-dependent transcription together with either SMARCA4 or GCN5L2\n Location: Nucleus\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that TFDP1 and TADA2B co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 810358, "gene_symbol_1": "TFDP1", "gene_symbol_2": "TADA2B", "detection_method": null}} +{"question_id": "PPIL1-0618", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: PAXIP1 (Q6ZW49)\n Function: Involved in DNA damage response and in transcriptional regulation through histone methyltransferase (HMT) complexes. Plays a role in early development. In DNA damage response is required for cell surv\n Location: Nucleus matrix\n\nProtein 2: CPAP (Q9HC77)\n Function: Plays an important role in cell division and centrosome function by participating in centriole duplication (PubMed:17681131, PubMed:20531387). Inhibits microtubule nucleation from the centrosome. Invo\n Location: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that PAXIP1 and CPAP co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1595871, "gene_symbol_1": "PAXIP1", "gene_symbol_2": "CPAP", "detection_method": null}} +{"question_id": "PPIL1-0619", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: SLF2 (Q8IX21)\n Function: Plays a role in the DNA damage response (DDR) pathway by regulating postreplication repair of UV-damaged DNA and genomic stability maintenance (PubMed:25931565). The SLF1-SLF2 complex acts to link RAD\n Location: Nucleus\n\nProtein 2: TAPBP (O15533)\n Function: Involved in the association of MHC class I with transporter associated with antigen processing (TAP) and in the assembly of MHC class I with peptide (peptide loading)\n Location: Endoplasmic reticulum membrane\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that SLF2 and TAPBP co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 585162, "gene_symbol_1": "SLF2", "gene_symbol_2": "TAPBP", "detection_method": null}} +{"question_id": "PPIL1-0620", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: CASP9 (P55211)\n Function: Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates effector caspase\n\nProtein 2: DGCR8 (Q8WYQ5)\n Function: Component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that i\n Location: Nucleus\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that CASP9 and DGCR8 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1643130, "gene_symbol_1": "CASP9", "gene_symbol_2": "DGCR8", "detection_method": null}} +{"question_id": "PPIL1-0621", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: PRKAB2 (O43741)\n Function: Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular \n\nProtein 2: MRPS34 (P82930)\n Function: Required for mitochondrial translation, plays a role in maintaining the stability of the small ribosomal subunit and the 12S rRNA that are required for mitoribosome formation\n Location: Mitochondrion\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that PRKAB2 and MRPS34 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1477624, "gene_symbol_1": "PRKAB2", "gene_symbol_2": "MRPS34", "detection_method": null}} +{"question_id": "PPIL1-0622", "task": "ppi-l1", "split": "fewshot", "difficulty": "hard", "context_text": "Protein 1: STING1 (Q86WV6)\n Function: Facilitator of innate immune signaling that acts as a sensor of cytosolic DNA from bacteria and viruses and promotes the production of type I interferon (IFN-alpha and IFN-beta) (PubMed:18724357, PubM\n Location: Endoplasmic reticulum membrane\n\nProtein 2: HAUS7 (Q99871)\n Function: Contributes to mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex\n Location: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome\n\nEvidence: Analysis of protein complex data indicates STING1 and HAUS7 are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1121552, "gene_symbol_1": "STING1", "gene_symbol_2": "HAUS7", "detection_method": null}} +{"question_id": "PPIL1-0623", "task": "ppi-l1", "split": "fewshot", "difficulty": "hard", "context_text": "Protein 1: UQCRC1 (P31930)\n Function: Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation. The resp\n Location: Mitochondrion inner membrane\n\nProtein 2: CCDC47 (Q96A33)\n Function: Component of the multi-pass translocon (MPT) complex that mediates insertion of multi-pass membrane proteins into the lipid bilayer of membranes (PubMed:32814900, PubMed:32820719, PubMed:36261522). Th\n Location: Endoplasmic reticulum membrane\n\nEvidence: Analysis of protein complex data indicates UQCRC1 and CCDC47 are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1596912, "gene_symbol_1": "UQCRC1", "gene_symbol_2": "CCDC47", "detection_method": null}} +{"question_id": "PPIL1-0624", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: STAT3 (P40763)\n Function: Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors (PubMed:10688651, PubMed:12359225, PubMed:12873986, PubMed:15194\n Location: Cytoplasm\n\nProtein 2: IFT27 (Q9BW83)\n Function: Small GTPase-like component of the intraflagellar transport (IFT) complex B that promotes the exit of the BBSome complex from cilia via its interaction with ARL6 (PubMed:25443296). Not involved in ent\n Location: Cell projection, cilium\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that STAT3 and IFT27 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1207700, "gene_symbol_1": "STAT3", "gene_symbol_2": "IFT27", "detection_method": null}} +{"question_id": "PPIL1-0625", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: CCND1 (P24385)\n Function: Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S tran\n Location: Nucleus\n\nProtein 2: SUPT4H1 (P63272)\n Function: Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II (PubMed:10075709, PubMed:10199401, PubMed:104\n Location: Nucleus\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that CCND1 and SUPT4H1 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 647614, "gene_symbol_1": "CCND1", "gene_symbol_2": "SUPT4H1", "detection_method": null}} +{"question_id": "PPIL1-0626", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: ORC6 (Q9Y5N6)\n Function: Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identifie\n Location: Nucleus\n\nProtein 2: GABRB3 (P28472)\n Function: Beta subunit of the heteropentameric ligand-gated chloride channel gated by gamma-aminobutyric acid (GABA), a major inhibitory neurotransmitter in the brain (PubMed:14993607, PubMed:18514161, PubMed:2\n Location: Postsynaptic cell membrane\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that ORC6 and GABRB3 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1113654, "gene_symbol_1": "ORC6", "gene_symbol_2": "GABRB3", "detection_method": null}} +{"question_id": "PPIL1-0627", "task": "ppi-l1", "split": "fewshot", "difficulty": "hard", "context_text": "Protein 1: SMARCD1 (Q96GM5)\n Function: Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry ou\n Location: Nucleus\n\nProtein 2: RXRA (P19793)\n Function: Receptor for retinoic acid that acts as a transcription factor (PubMed:10874028, PubMed:11162439, PubMed:11915042, PubMed:37478846). Forms homo- or heterodimers with retinoic acid receptors (RARs) and\n Location: Nucleus\n\nEvidence: Analysis of protein complex data indicates SMARCD1 and RXRA are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1169192, "gene_symbol_1": "SMARCD1", "gene_symbol_2": "RXRA", "detection_method": null}} +{"question_id": "PPIL1-0628", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: PDGFD (Q9GZP0)\n Function: Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Play\n Location: Secreted\n\nProtein 2: MED13 (Q9UHV7)\n Function: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene\n Location: Nucleus\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that PDGFD and MED13 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 928110, "gene_symbol_1": "PDGFD", "gene_symbol_2": "MED13", "detection_method": null}} +{"question_id": "PPIL1-0629", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: EP400 (Q96L91)\n Function: Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification\n Location: Nucleus\n\nProtein 2: CRBN (Q96SW2)\n Function: Substrate recognition component of a DCX (DDB1-CUL4-X-box) E3 protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins, such as MEIS2, ILF2 or \n Location: Cytoplasm\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that EP400 and CRBN co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 809999, "gene_symbol_1": "EP400", "gene_symbol_2": "CRBN", "detection_method": null}} +{"question_id": "PPIL1-0630", "task": "ppi-l1", "split": "fewshot", "difficulty": "hard", "context_text": "Protein 1: EXOSC7 (Q15024)\n Function: Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA \n Location: Nucleus, nucleolus\n\nProtein 2: TRAPPC2L (Q9UL33)\n Function: Plays a role in vesicular transport from endoplasmic reticulum to Golgi\n Location: Cytoplasm, perinuclear region\n\nEvidence: Analysis of protein complex data indicates EXOSC7 and TRAPPC2L are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1636424, "gene_symbol_1": "EXOSC7", "gene_symbol_2": "TRAPPC2L", "detection_method": null}} +{"question_id": "PPIL1-0631", "task": "ppi-l1", "split": "fewshot", "difficulty": "hard", "context_text": "Protein 1: APH1B (Q8WW43)\n Function: Probable subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral proteins such as Notch receptors and APP (amyloid-beta precursor protein)\n Location: Membrane\n\nProtein 2: SMARCC1 (Q92922)\n Function: Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry ou\n Location: Nucleus\n\nEvidence: Analysis of protein complex data indicates APH1B and SMARCC1 are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1615707, "gene_symbol_1": "APH1B", "gene_symbol_2": "SMARCC1", "detection_method": null}} +{"question_id": "PPIL1-0632", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: EIF3F (O00303)\n Function: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773, PubMed:274628\n Location: Cytoplasm\n\nProtein 2: CENPK (Q9BS16)\n Function: Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be\n Location: Nucleus\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that EIF3F and CENPK do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1584812, "gene_symbol_1": "EIF3F", "gene_symbol_2": "CENPK", "detection_method": null}} +{"question_id": "PPIL1-0633", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: LSM11 (P83369)\n Function: Component of the U7 snRNP complex that is involved in the histone 3'-end pre-mRNA processing (PubMed:11574479, PubMed:16914750, PubMed:33230297). Increases U7 snRNA levels but not histone 3'-end pre-m\n Location: Nucleus\n\nProtein 2: F2 (P00734)\n Function: Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis,\n Location: Secreted, extracellular space\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that LSM11 and F2 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 784412, "gene_symbol_1": "LSM11", "gene_symbol_2": "F2", "detection_method": null}} +{"question_id": "PPIL1-0634", "task": "ppi-l1", "split": "fewshot", "difficulty": "hard", "context_text": "Protein 1: INTS5 (Q6P9B9)\n Function: Component of the integrator complex, a multiprotein complex that terminates RNA polymerase II (Pol II) transcription in the promoter-proximal region of genes (PubMed:33243860, PubMed:38570683). The in\n Location: Nucleus\n\nProtein 2: ITGA9 (Q13797)\n Function: Integrin alpha-9/beta-1 (ITGA9:ITGB1) is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. ITGA9:ITGB1 may play a crucial role in SVEP1/polydo\n Location: Membrane\n\nEvidence: Analysis of protein complex data indicates INTS5 and ITGA9 are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1030430, "gene_symbol_1": "INTS5", "gene_symbol_2": "ITGA9", "detection_method": null}} +{"question_id": "PPIL1-0635", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: C6 (P13671)\n Function: Component of the membrane attack complex (MAC), a multiprotein complex activated by the complement cascade, which inserts into a target cell membrane and forms a pore, leading to target cell membrane \n Location: Secreted\n\nProtein 2: CDK14 (O94921)\n Function: Serine/threonine-protein kinase involved in the control of the eukaryotic cell cycle, whose activity is controlled by an associated cyclin. Acts as a cell-cycle regulator of Wnt signaling pathway duri\n Location: Cell membrane\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that C6 and CDK14 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 796880, "gene_symbol_1": "C6", "gene_symbol_2": "CDK14", "detection_method": null}} +{"question_id": "PPIL1-0636", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: NABP1 (Q96AH0)\n Function: Component of the SOSS complex, a multiprotein complex that functions downstream of the MRN complex to promote DNA repair and G2/M checkpoint. In the SOSS complex, acts as a sensor of single-stranded D\n Location: Nucleus\n\nProtein 2: NELFA (Q9H3P2)\n Function: Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex\n Location: Nucleus\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that NABP1 and NELFA co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 527546, "gene_symbol_1": "NABP1", "gene_symbol_2": "NELFA", "detection_method": null}} +{"question_id": "PPIL1-0637", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: BMAL2 (Q8WYA1)\n Function: Transcriptional activator which forms a core component of the circadian clock. The circadian clock, an internal time-keeping system, regulates various physiological processes through the generation of\n Location: Nucleus\n\nProtein 2: INIP (Q9NRY2)\n Function: Component of the SOSS complex, a multiprotein complex that functions downstream of the MRN complex to promote DNA repair and G2/M checkpoint. The SOSS complex associates with single-stranded DNA at DN\n Location: Nucleus\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that BMAL2 and INIP co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1159122, "gene_symbol_1": "BMAL2", "gene_symbol_2": "INIP", "detection_method": null}} +{"question_id": "PPIL1-0638", "task": "ppi-l1", "split": "fewshot", "difficulty": "hard", "context_text": "Protein 1: ATP5MK (Q96IX5)\n Function: Subunit k, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generate\n Location: Mitochondrion membrane\n\nProtein 2: CHD3 (Q12873)\n Function: ATP-dependent chromatin-remodeling factor that binds and distorts nucleosomal DNA (PubMed:28977666). Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of\n Location: Nucleus, PML body\n\nEvidence: Analysis of protein complex data indicates ATP5MK and CHD3 are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 614396, "gene_symbol_1": "ATP5MK", "gene_symbol_2": "CHD3", "detection_method": null}} +{"question_id": "PPIL1-0639", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: KRIT1 (O00522)\n Function: Component of the CCM signaling pathway which is a crucial regulator of heart and vessel formation and integrity (By similarity). Negative regulator of angiogenesis. Inhibits endothelial proliferation,\n Location: Cytoplasm, cytoskeleton\n\nProtein 2: IFT43 (Q96FT9)\n Function: As a component of IFT complex A (IFT-A), a complex required for retrograde ciliary transport and entry into cilia of G protein-coupled receptors (GPCRs), it is involved in ciliogenesis (PubMed:2840094\n Location: Cytoplasm, cytoskeleton\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that KRIT1 and IFT43 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 757193, "gene_symbol_1": "KRIT1", "gene_symbol_2": "IFT43", "detection_method": null}} +{"question_id": "PPIL1-0640", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: RNASEH2B (Q5TBB1)\n Function: Non catalytic subunit of RNase H2, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki f\n Location: Nucleus\n\nProtein 2: GPIHBP1 (Q8IV16)\n Function: Mediates the transport of lipoprotein lipase LPL from the basolateral to the apical surface of endothelial cells in capillaries (By similarity). Anchors LPL on the surface of endothelial cells in the \n Location: Apical cell membrane\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that RNASEH2B and GPIHBP1 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1439253, "gene_symbol_1": "RNASEH2B", "gene_symbol_2": "GPIHBP1", "detection_method": null}} +{"question_id": "PPIL1-0641", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: NFKB1 (P19838)\n Function: NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to\n Location: Cytoplasm\n\nProtein 2: PIGG (Q5H8A4)\n Function: Catalytic subunit of the ethanolamine phosphate transferase 2 complex that transfers an ethanolamine phosphate (EtNP) from a phosphatidylethanolamine (PE) to the 6-OH position of the second alpha-1,6-\n Location: Endoplasmic reticulum membrane\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that NFKB1 and PIGG do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1131237, "gene_symbol_1": "NFKB1", "gene_symbol_2": "PIGG", "detection_method": null}} +{"question_id": "PPIL1-0642", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: TP53 (P04637)\n Function: Multifunctional transcription factor that induces cell cycle arrest, DNA repair or apoptosis upon binding to its target DNA sequence (PubMed:11025664, PubMed:12524540, PubMed:12810724, PubMed:15186775\n Location: Cytoplasm\n\nProtein 2: TSHB (P01222)\n Function: Indispensable for the control of thyroid structure and metabolism\n Location: Secreted\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that TP53 and TSHB do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1212043, "gene_symbol_1": "TP53", "gene_symbol_2": "TSHB", "detection_method": null}} +{"question_id": "PPIL1-0643", "task": "ppi-l1", "split": "fewshot", "difficulty": "hard", "context_text": "Protein 1: EMC4 (Q5J8M3)\n Function: Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins (PubMed:29242\n Location: Endoplasmic reticulum membrane\n\nProtein 2: ATP10B (O94823)\n Function: Catalytic component of a P4-ATPase flippase complex, which catalyzes the hydrolysis of ATP coupled to the transport of glucosylceramide (GlcCer) from the outer to the inner leaflet of lysosome membran\n Location: Late endosome membrane\n\nEvidence: Analysis of protein complex data indicates EMC4 and ATP10B are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1511207, "gene_symbol_1": "EMC4", "gene_symbol_2": "ATP10B", "detection_method": null}} +{"question_id": "PPIL1-0644", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: GCLC (P48506)\n Function: Catalyzes the ATP-dependent ligation of L-glutamate and L-cysteine and participates in the first and rate-limiting step in glutathione biosynthesis\n\nProtein 2: ANAPC4 (Q9UJX5)\n Function: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle (PubMed:18485873)\n Location: Nucleus\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that GCLC and ANAPC4 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1167516, "gene_symbol_1": "GCLC", "gene_symbol_2": "ANAPC4", "detection_method": null}} +{"question_id": "PPIL1-0645", "task": "ppi-l1", "split": "fewshot", "difficulty": "hard", "context_text": "Protein 1: RTRAF (Q9Y224)\n Function: Accessory subunit of the tRNA-splicing ligase complex that acts by directly joining spliced tRNA halves to mature-sized tRNAs by incorporating the precursor-derived splice junction phosphate into the \n Location: Nucleus\n\nProtein 2: NAA25 (Q14CX7)\n Function: Non-catalytic subunit of the NatB complex which catalyzes acetylation of the N-terminal methionine residues of peptides beginning with Met-Asp, Met-Glu, Met-Asn and Met-Gln. May play a role in normal \n Location: Cytoplasm\n\nEvidence: Analysis of protein complex data indicates RTRAF and NAA25 are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1456849, "gene_symbol_1": "RTRAF", "gene_symbol_2": "NAA25", "detection_method": null}} +{"question_id": "PPIL1-0646", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: UBE2V1 (Q13404)\n Function: Has no ubiquitin ligase activity on its own. The UBE2V1-UBE2N heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through Lys-63. This type of poly-ubiquitinatio\n Location: Nucleus\n\nProtein 2: SMARCD1 (Q96GM5)\n Function: Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry ou\n Location: Nucleus\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that UBE2V1 and SMARCD1 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1163992, "gene_symbol_1": "UBE2V1", "gene_symbol_2": "SMARCD1", "detection_method": null}} +{"question_id": "PPIL1-0647", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: UQCR10 (Q9UDW1)\n Function: Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation. The resp\n Location: Mitochondrion inner membrane\n\nProtein 2: FRAT1 (Q92837)\n Function: Positively regulates the Wnt signaling pathway by stabilizing beta-catenin through the association with GSK-3. May play a role in tumor progression and collaborate with PIM1 and MYC in lymphomagenesis\n Location: Cytoplasm\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that UQCR10 and FRAT1 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1576954, "gene_symbol_1": "UQCR10", "gene_symbol_2": "FRAT1", "detection_method": null}} +{"question_id": "PPIL1-0648", "task": "ppi-l1", "split": "fewshot", "difficulty": "hard", "context_text": "Protein 1: EIF2S3 (P41091)\n Function: Member of the eIF2 complex that functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA (PubMed:31836389). This complex binds to a 40S ribosomal subu\n Location: Cytoplasm, cytosol\n\nProtein 2: EIF4G3 (O43432)\n Function: Component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome (PubMed\n\nEvidence: Analysis of protein complex data indicates EIF2S3 and EIF4G3 are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1081877, "gene_symbol_1": "EIF2S3", "gene_symbol_2": "EIF4G3", "detection_method": null}} +{"question_id": "PPIL1-0649", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: MMS19 (Q96T76)\n Function: Key component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into apoproteins specifically involved in DNA m\n Location: Nucleus\n\nProtein 2: DPM3 (Q9P2X0)\n Function: Stabilizer subunit of the dolichol-phosphate mannose (DPM) synthase complex; tethers catalytic subunit DPM1 to the endoplasmic reticulum\n Location: Endoplasmic reticulum membrane\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that MMS19 and DPM3 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1624622, "gene_symbol_1": "MMS19", "gene_symbol_2": "DPM3", "detection_method": null}} +{"question_id": "PPIL1-0650", "task": "ppi-l1", "split": "fewshot", "difficulty": "hard", "context_text": "Protein 1: CACNB2 (Q08289)\n Function: Beta subunit of voltage-dependent calcium channels which contributes to the function of the calcium channel by increasing peak calcium current (By similarity). Plays a role in shifting voltage depende\n Location: Cell membrane, sarcolemma\n\nProtein 2: AP4B1 (Q9Y6B7)\n Function: Component of the adaptor protein complex 4 (AP-4). Adaptor protein complexes are vesicle coat components involved both in vesicle formation and cargo selection. They control the vesicular transport of\n Location: Golgi apparatus, trans-Golgi network membrane\n\nEvidence: Analysis of protein complex data indicates CACNB2 and AP4B1 are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 770567, "gene_symbol_1": "CACNB2", "gene_symbol_2": "AP4B1", "detection_method": null}} +{"question_id": "PPIL1-0651", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: AP4M1 (O00189)\n Function: Component of the adaptor protein complex 4 (AP-4). Adaptor protein complexes are vesicle coat components involved both in vesicle formation and cargo selection. They control the vesicular transport of\n Location: Golgi apparatus, trans-Golgi network membrane\n\nProtein 2: SNF8 (Q96H20)\n Function: Component of the endosomal sorting complex required for transport II (ESCRT-II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs, and plays \n Location: Cytoplasm\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that AP4M1 and SNF8 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 827417, "gene_symbol_1": "AP4M1", "gene_symbol_2": "SNF8", "detection_method": null}} +{"question_id": "PPIL1-0652", "task": "ppi-l1", "split": "fewshot", "difficulty": "hard", "context_text": "Protein 1: BIK (Q13323)\n Function: Accelerates programmed cell death. Association to the apoptosis repressors Bcl-X(L), BHRF1, Bcl-2 or its adenovirus homolog E1B 19k protein suppresses this death-promoting activity. Does not interact \n Location: Endomembrane system\n\nProtein 2: JADE2 (Q9NQC1)\n Function: Scaffold subunit of some HBO1 complexes, which have a histone H4 acetyltransferase activity (PubMed:16387653). Acts as an E3 ubiquitin-protein ligase mediating the ubiquitination and subsequent protea\n\nEvidence: Analysis of protein complex data indicates BIK and JADE2 are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 694230, "gene_symbol_1": "BIK", "gene_symbol_2": "JADE2", "detection_method": null}} +{"question_id": "PPIL1-0653", "task": "ppi-l1", "split": "fewshot", "difficulty": "hard", "context_text": "Protein 1: EXOC7 (Q9UPT5)\n Function: Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane. In adipocytes, plays a crucial role in targeting SLC2A4 vesicle to the plasma me\n Location: Cytoplasm, cytosol\n\nProtein 2: CSF2RB (P32927)\n Function: Cell surface receptor that plays a role in immune response and controls the production and differentiation of hematopoietic progenitor cells into lineage-restricted cells. Acts by forming an heterodim\n Location: Membrane\n\nEvidence: Analysis of protein complex data indicates EXOC7 and CSF2RB are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1017003, "gene_symbol_1": "EXOC7", "gene_symbol_2": "CSF2RB", "detection_method": null}} +{"question_id": "PPIL1-0654", "task": "ppi-l1", "split": "fewshot", "difficulty": "hard", "context_text": "Protein 1: TRAPPC2 (P0DI81)\n Function: Prevents transcriptional repression and induction of cell death by ENO1 (By similarity). May play a role in vesicular transport from endoplasmic reticulum to Golgi\n Location: Cytoplasm, perinuclear region\n\nProtein 2: GRIN2C (Q14957)\n Function: Component of N-methyl-D-aspartate (NMDA) receptors (NMDARs) that function as heterotetrameric, ligand-gated cation channels with high calcium permeability and voltage-dependent block by Mg(2+) (PubMed\n Location: Cell membrane\n\nEvidence: Analysis of protein complex data indicates TRAPPC2 and GRIN2C are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1056141, "gene_symbol_1": "TRAPPC2", "gene_symbol_2": "GRIN2C", "detection_method": null}} +{"question_id": "PPIL1-0655", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: COL4A6 (Q14031)\n Function: Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen\n Location: Secreted, extracellular space, extracellular matrix, basement membrane\n\nProtein 2: CDK5R2 (Q13319)\n Function: Activator of CDK5/TPKII\n Location: Cell membrane\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that COL4A6 and CDK5R2 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1371933, "gene_symbol_1": "COL4A6", "gene_symbol_2": "CDK5R2", "detection_method": null}} +{"question_id": "PPIL1-0656", "task": "ppi-l1", "split": "fewshot", "difficulty": "hard", "context_text": "Protein 1: KRT14 (P02533)\n Function: The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filam\n Location: Cytoplasm\n\nProtein 2: RPP25 (Q9BUL9)\n Function: Component of ribonuclease P, a ribonucleoprotein complex that generates mature tRNA molecules by cleaving their 5'-ends (PubMed:12003489, PubMed:16723659, PubMed:30454648). Also a component of the MRP\n Location: Nucleus, nucleolus\n\nEvidence: Analysis of protein complex data indicates KRT14 and RPP25 are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1272656, "gene_symbol_1": "KRT14", "gene_symbol_2": "RPP25", "detection_method": null}} +{"question_id": "PPIL1-0657", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: SMARCB1 (Q12824)\n Function: Core component of the BAF (hSWI/SNF) complex. This ATP-dependent chromatin-remodeling complex plays important roles in cell proliferation and differentiation, in cellular antiviral activities and inhi\n Location: Nucleus\n\nProtein 2: FOSL1 (P15407)\n Location: Nucleus\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that SMARCB1 and FOSL1 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 551552, "gene_symbol_1": "SMARCB1", "gene_symbol_2": "FOSL1", "detection_method": null}} +{"question_id": "PPIL1-0658", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: EIF4E (P06730)\n Function: Acts in the cytoplasm to initiate and regulate protein synthesis and is required in the nucleus for export of a subset of mRNAs from the nucleus to the cytoplasm which promotes processes such as RNA c\n Location: Cytoplasm, P-body\n\nProtein 2: PADI6 (Q6TGC4)\n Function: Structural constituent of cytoplasmic lattices, which plays a key role in early embryonic development (PubMed:37922900). Cytoplasmic lattices consist in fibrous structures found in the cytoplasm of oo\n Location: Cytoplasm\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that EIF4E and PADI6 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 677507, "gene_symbol_1": "EIF4E", "gene_symbol_2": "PADI6", "detection_method": null}} +{"question_id": "PPIL1-0659", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: PNPT1 (Q8TCS8)\n Function: RNA-binding protein implicated in numerous RNA metabolic processes (PubMed:29967381, PubMed:39019044). Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'-to-5' \n Location: Cytoplasm\n\nProtein 2: SKA1 (Q96BD8)\n Function: Component of the SKA complex, a microtubule plus end-binding complex of the outer kinetochore that stabilizes spindle microtubule-kinetochore attachments, promotes alignment of chromosomes at the mito\n Location: Cytoplasm, cytoskeleton, spindle\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that PNPT1 and SKA1 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 765524, "gene_symbol_1": "PNPT1", "gene_symbol_2": "SKA1", "detection_method": null}} +{"question_id": "PPIL1-0660", "task": "ppi-l1", "split": "val", "difficulty": "hard", "context_text": "Protein 1: CHMP4C (Q96CF2)\n Function: Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into\n Location: Cytoplasm, cytosol\n\nProtein 2: PIGO (Q8TEQ8)\n Function: Catalytic subunit of the ethanolamine phosphate transferase 3 complex that transfers an ethanolamine phosphate (EtNP) from a phosphatidylethanolamine (PE) to the 6-OH position of the third alpha-1,2-l\n Location: Endoplasmic reticulum membrane\n\nEvidence: Analysis of protein complex data indicates CHMP4C and PIGO are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1115556, "gene_symbol_1": "CHMP4C", "gene_symbol_2": "PIGO", "detection_method": null}} +{"question_id": "PPIL1-0661", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: NDC80 (O14777)\n Function: Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity (PubMed:12351790, PubMed:14654001, PubMed:146991\n Location: Nucleus\n\nProtein 2: NCOA2 (Q15596)\n Function: Transcriptional coactivator for steroid receptors and nuclear receptors (PubMed:23508108, PubMed:8670870, PubMed:9430642, PubMed:22504882, PubMed:26553876). Coactivator of the steroid binding domain (\n Location: Nucleus\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that NDC80 and NCOA2 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1187523, "gene_symbol_1": "NDC80", "gene_symbol_2": "NCOA2", "detection_method": null}} +{"question_id": "PPIL1-0662", "task": "ppi-l1", "split": "val", "difficulty": "hard", "context_text": "Protein 1: TADA1 (Q96BN2)\n Function: Probably involved in transcriptional regulation\n Location: Nucleus\n\nProtein 2: F8 (P00451)\n Function: Factor VIII, along with calcium and phospholipid, acts as a cofactor for F9/factor IXa when it converts F10/factor X to the activated form, factor Xa\n Location: Secreted, extracellular space\n\nEvidence: Analysis of protein complex data indicates TADA1 and F8 are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1045679, "gene_symbol_1": "TADA1", "gene_symbol_2": "F8", "detection_method": null}} +{"question_id": "PPIL1-0663", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: NOP10 (Q9NPE3)\n Function: Required for ribosome biogenesis and telomere maintenance. Part of the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA (PubMed:32554502). This\n Location: Nucleus, nucleolus\n\nProtein 2: MED23 (Q9ULK4)\n Function: Required for transcriptional activation subsequent to the assembly of the pre-initiation complex (By similarity). Component of the Mediator complex, a coactivator involved in the regulated transcripti\n Location: Nucleus\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that NOP10 and MED23 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 791961, "gene_symbol_1": "NOP10", "gene_symbol_2": "MED23", "detection_method": null}} +{"question_id": "PPIL1-0664", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: TSHB (P01222)\n Function: Indispensable for the control of thyroid structure and metabolism\n Location: Secreted\n\nProtein 2: KCNE2 (Q9Y6J6)\n Function: Ancillary protein that functions as a regulatory subunit of the voltage-gated potassium (Kv) channel complex composed of pore-forming and potassium-conducting alpha subunits and of regulatory beta sub\n Location: Cell membrane\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that TSHB and KCNE2 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1695674, "gene_symbol_1": "TSHB", "gene_symbol_2": "KCNE2", "detection_method": null}} +{"question_id": "PPIL1-0665", "task": "ppi-l1", "split": "val", "difficulty": "hard", "context_text": "Protein 1: AP3S2 (P59780)\n Function: Part of the AP-3 complex, an adaptor-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding\n Location: Golgi apparatus\n\nProtein 2: ICE1 (Q9Y2F5)\n Function: Component of the little elongation complex (LEC), a complex required to regulate small nuclear RNA (snRNA) gene transcription by RNA polymerase II and III (PubMed:22195968, PubMed:23932780). Specifica\n Location: Nucleus\n\nEvidence: Analysis of protein complex data indicates AP3S2 and ICE1 are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 962255, "gene_symbol_1": "AP3S2", "gene_symbol_2": "ICE1", "detection_method": null}} +{"question_id": "PPIL1-0666", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: PAM16 (Q9Y3D7)\n Function: Regulates ATP-dependent protein translocation into the mitochondrial matrix. Inhibits DNAJC19 stimulation of HSPA9/Mortalin ATPase activity\n Location: Mitochondrion inner membrane\n\nProtein 2: KCNE2 (Q9Y6J6)\n Function: Ancillary protein that functions as a regulatory subunit of the voltage-gated potassium (Kv) channel complex composed of pore-forming and potassium-conducting alpha subunits and of regulatory beta sub\n Location: Cell membrane\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that PAM16 and KCNE2 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1219946, "gene_symbol_1": "PAM16", "gene_symbol_2": "KCNE2", "detection_method": null}} +{"question_id": "PPIL1-0667", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: EXOSC5 (Q9NQT4)\n Function: Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA \n Location: Nucleus, nucleolus\n\nProtein 2: ELP3 (Q9H9T3)\n Function: Catalytic tRNA acetyltransferase subunit of the elongator complex which is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl\n Location: Cytoplasm\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that EXOSC5 and ELP3 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1196356, "gene_symbol_1": "EXOSC5", "gene_symbol_2": "ELP3", "detection_method": null}} +{"question_id": "PPIL1-0668", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: ACTB (P60709)\n Function: Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed:25255767, PubMed:29581253). Actin exists in both monomeric (\n Location: Cytoplasm, cytoskeleton\n\nProtein 2: LAMC1 (P11047)\n Function: Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other ext\n Location: Secreted, extracellular space, extracellular matrix, basement membrane\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that ACTB and LAMC1 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 672157, "gene_symbol_1": "ACTB", "gene_symbol_2": "LAMC1", "detection_method": null}} +{"question_id": "PPIL1-0669", "task": "ppi-l1", "split": "val", "difficulty": "hard", "context_text": "Protein 1: CD3G (P09693)\n Function: Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-medi\n Location: Cell membrane\n\nProtein 2: NRXN1 (P58400)\n Function: Neuronal cell surface protein involved in cell recognition and cell adhesion by forming intracellular junctions through binding to neuroligins (By similarity). Plays a role in formation of synaptic ju\n Location: Presynaptic cell membrane\n\nEvidence: Analysis of protein complex data indicates CD3G and NRXN1 are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 553818, "gene_symbol_1": "CD3G", "gene_symbol_2": "NRXN1", "detection_method": null}} +{"question_id": "PPIL1-0670", "task": "ppi-l1", "split": "val", "difficulty": "hard", "context_text": "Protein 1: ATF4 (P18848)\n Function: Transcription factor that binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3') and displays two biological functions, as regulator of metabolic and redox processes under normal cel\n Location: Nucleus\n\nProtein 2: ATF5 (Q9Y2D1)\n Function: Transcription factor that either stimulates or represses gene transcription through binding of different DNA regulatory elements such as cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'),\n Location: Cytoplasm\n\nEvidence: Analysis of protein complex data indicates ATF4 and ATF5 are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1593909, "gene_symbol_1": "ATF4", "gene_symbol_2": "ATF5", "detection_method": null}} +{"question_id": "PPIL1-0671", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: HAUS5 (O94927)\n Function: Contributes to mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex\n Location: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome\n\nProtein 2: SMC3 (Q9UQE7)\n Function: Central component of cohesin, a complex required for chromosome cohesion during the cell cycle. The cohesin complex may form a large proteinaceous ring within which sister chromatids can be trapped. A\n Location: Nucleus\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that HAUS5 and SMC3 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 961127, "gene_symbol_1": "HAUS5", "gene_symbol_2": "SMC3", "detection_method": null}} +{"question_id": "PPIL1-0672", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: DDX21 (Q9NR30)\n Function: RNA helicase that acts as a sensor of the transcriptional status of both RNA polymerase (Pol) I and II: promotes ribosomal RNA (rRNA) processing and transcription from polymerase II (Pol II) (PubMed:2\n Location: Nucleus, nucleolus\n\nProtein 2: KNL1 (Q8NG31)\n Function: Acts as a component of the outer kinetochore KNL1 complex that serves as a docking point for spindle assembly checkpoint components and mediates microtubule-kinetochore interactions (PubMed:15502821, \n Location: Nucleus\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that DDX21 and KNL1 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1464876, "gene_symbol_1": "DDX21", "gene_symbol_2": "KNL1", "detection_method": null}} +{"question_id": "PPIL1-0673", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: COL6A1 (P12109)\n Function: Collagen VI acts as a cell-binding protein\n Location: Secreted, extracellular space, extracellular matrix\n\nProtein 2: THOC5 (Q13769)\n Function: Component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspl\n Location: Nucleus\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that COL6A1 and THOC5 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 913326, "gene_symbol_1": "COL6A1", "gene_symbol_2": "THOC5", "detection_method": null}} +{"question_id": "PPIL1-0674", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: TRAPPC9 (Q96Q05)\n Function: Functions as an activator of NF-kappa-B through increased phosphorylation of the IKK complex. May function in neuronal cells differentiation. May play a role in vesicular transport from endoplasmic re\n Location: Golgi apparatus, cis-Golgi network\n\nProtein 2: CACNA2D1 (P54289)\n Function: The alpha-2/delta subunit of voltage-dependent calcium channels regulates calcium current density and activation/inactivation kinetics of the calcium channel (PubMed:35293990). Plays an important role\n Location: Membrane\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that TRAPPC9 and CACNA2D1 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1029440, "gene_symbol_1": "TRAPPC9", "gene_symbol_2": "CACNA2D1", "detection_method": null}} +{"question_id": "PPIL1-0675", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: GEMIN7 (Q9H840)\n Function: The SMN complex catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, and thereby plays an important role in the splicing of cellular pre-mRNAs. \n Location: Nucleus, nucleoplasm\n\nProtein 2: VPS28 (Q9UK41)\n Function: Component of the ESCRT-I complex, a regulator of vesicular trafficking process\n Location: Cell membrane\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that GEMIN7 and VPS28 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1504271, "gene_symbol_1": "GEMIN7", "gene_symbol_2": "VPS28", "detection_method": null}} +{"question_id": "PPIL1-0676", "task": "ppi-l1", "split": "val", "difficulty": "hard", "context_text": "Protein 1: POT1 (Q9NUX5)\n Function: Component of the telomerase ribonucleoprotein (RNP) complex that is essential for the replication of chromosome termini. Is a component of the double-stranded telomeric DNA-binding TRF1 complex which \n Location: Nucleus\n\nProtein 2: IL10RB (Q08334)\n Function: Shared cell surface receptor required for the activation of five class 2 cytokines: IL10, IL22, IL26, IL28, and IFNL1. The IFNLR1/IL10RB dimer is a receptor for the cytokine ligands IFNL2 and IFNL3 an\n Location: Membrane\n\nEvidence: Analysis of protein complex data indicates POT1 and IL10RB are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 675039, "gene_symbol_1": "POT1", "gene_symbol_2": "IL10RB", "detection_method": null}} +{"question_id": "PPIL1-0677", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: KCNJ5 (P48544)\n Function: Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration o\n Location: Membrane\n\nProtein 2: SMARCD3 (Q6STE5)\n Function: Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry ou\n Location: Nucleus\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that KCNJ5 and SMARCD3 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1468692, "gene_symbol_1": "KCNJ5", "gene_symbol_2": "SMARCD3", "detection_method": null}} +{"question_id": "PPIL1-0678", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: FAM98A (Q8NCA5)\n Function: Positively stimulates PRMT1-induced protein arginine methylation (PubMed:28040436). Involved in skeletal homeostasis (By similarity). Positively regulates lysosome peripheral distribution and ruffled \n\nProtein 2: SNRNP27 (Q8WVK2)\n Function: May play a role in mRNA splicing\n Location: Nucleus\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that FAM98A and SNRNP27 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1398611, "gene_symbol_1": "FAM98A", "gene_symbol_2": "SNRNP27", "detection_method": null}} +{"question_id": "PPIL1-0679", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: VCL (P18206)\n Function: Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex.\n Location: Cell membrane\n\nProtein 2: BATF (Q16520)\n Function: AP-1 family transcription factor that controls the differentiation of lineage-specific cells in the immune system: specifically mediates the differentiation of T-helper 17 cells (Th17), follicular T-h\n Location: Nucleus\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that VCL and BATF do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1611607, "gene_symbol_1": "VCL", "gene_symbol_2": "BATF", "detection_method": null}} +{"question_id": "PPIL1-0680", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: KANSL1 (Q7Z3B3)\n Function: Non-catalytic component of the NSL histone acetyltransferase complex, a multiprotein complex that mediates histone H4 acetylation at 'Lys-5'- and 'Lys-8' (H4K5ac and H4K8ac) at transcription start sit\n Location: Nucleus\n\nProtein 2: FANCM (Q8IYD8)\n Function: DNA-dependent ATPase component of the Fanconi anemia (FA) core complex (PubMed:16116422). Required for the normal activation of the FA pathway, leading to monoubiquitination of the FANCI-FANCD2 comple\n Location: Nucleus\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that KANSL1 and FANCM co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1130985, "gene_symbol_1": "KANSL1", "gene_symbol_2": "FANCM", "detection_method": null}} +{"question_id": "PPIL1-0681", "task": "ppi-l1", "split": "val", "difficulty": "hard", "context_text": "Protein 1: CYBB (P04839)\n Function: Catalytic subunit of the phagocyte NADPH oxidase complex that mediates the transfer of electrons from cytosolic NADPH to O2 to produce the superoxide anion (O2(-)) (PubMed:15338276, PubMed:36241643, P\n Location: Cell membrane\n\nProtein 2: THOC3 (Q96J01)\n Function: Component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspl\n Location: Nucleus\n\nEvidence: Analysis of protein complex data indicates CYBB and THOC3 are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 974080, "gene_symbol_1": "CYBB", "gene_symbol_2": "THOC3", "detection_method": null}} +{"question_id": "PPIL1-0682", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: LSM4 (Q9Y4Z0)\n Function: Plays a role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex) (Pub\n Location: Nucleus\n\nProtein 2: DAP3 (P51398)\n Function: As a component of the mitochondrial small ribosomal subunit, it plays a role in the translation of mitochondrial mRNAs (PubMed:39701103). Involved in mediating interferon-gamma-induced cell death (Pub\n Location: Mitochondrion\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that LSM4 and DAP3 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1297112, "gene_symbol_1": "LSM4", "gene_symbol_2": "DAP3", "detection_method": null}} +{"question_id": "PPIL1-0683", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: ITGAM (P11215)\n Function: Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PubMed:2\n Location: Cell membrane\n\nProtein 2: ITGA3 (P26006)\n Function: Integrin alpha-3/beta-1 is a receptor for fibronectin, laminin, collagen, epiligrin, thrombospondin and CSPG4. Integrin alpha-3/beta-1 provides a docking site for FAP (seprase) at invadopodia plasma m\n Location: Cell membrane\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that ITGAM and ITGA3 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 523718, "gene_symbol_1": "ITGAM", "gene_symbol_2": "ITGA3", "detection_method": null}} +{"question_id": "PPIL1-0684", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: TSEN54 (Q7Z6J9)\n Function: Non-catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5' and 3' splice site\n Location: Nucleus\n\nProtein 2: INTU (Q9ULD6)\n Function: Plays a key role in ciliogenesis and embryonic development. Regulator of cilia formation by controlling the organization of the apical actin cytoskeleton and the positioning of the basal bodies at the\n Location: Cytoplasm\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that TSEN54 and INTU do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 837409, "gene_symbol_1": "TSEN54", "gene_symbol_2": "INTU", "detection_method": null}} +{"question_id": "PPIL1-0685", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: INTS4 (Q96HW7)\n Function: Component of the integrator complex, a multiprotein complex that terminates RNA polymerase II (Pol II) transcription in the promoter-proximal region of genes (PubMed:29471365, PubMed:33243860, PubMed:\n Location: Nucleus\n\nProtein 2: F9 (P00740)\n Function: Factor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca(2+) ions, phospholipid\n Location: Secreted\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that INTS4 and F9 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 656065, "gene_symbol_1": "INTS4", "gene_symbol_2": "F9", "detection_method": null}} +{"question_id": "PPIL1-0686", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: TP53 (P04637)\n Function: Multifunctional transcription factor that induces cell cycle arrest, DNA repair or apoptosis upon binding to its target DNA sequence (PubMed:11025664, PubMed:12524540, PubMed:12810724, PubMed:15186775\n Location: Cytoplasm\n\nProtein 2: CACNG1 (Q06432)\n Function: Regulatory subunit of the voltage-gated calcium channel that gives rise to L-type calcium currents in skeletal muscle. Regulates channel inactivation kinetics\n Location: Cell membrane, sarcolemma\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that TP53 and CACNG1 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1306368, "gene_symbol_1": "TP53", "gene_symbol_2": "CACNG1", "detection_method": null}} +{"question_id": "PPIL1-0687", "task": "ppi-l1", "split": "val", "difficulty": "hard", "context_text": "Protein 1: GSK3B (P49841)\n Function: Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosph\n Location: Cytoplasm\n\nProtein 2: VTN (P04004)\n Function: Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family \n Location: Secreted, extracellular space\n\nEvidence: Analysis of protein complex data indicates GSK3B and VTN are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 674124, "gene_symbol_1": "GSK3B", "gene_symbol_2": "VTN", "detection_method": null}} +{"question_id": "PPIL1-0688", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: USP22 (Q9UPT9)\n Function: Deubiquitinase that plays a role in several cellular processes including transcriptional regulation, cell cycle progression or innate immunity. As part of the transcription regulatory histone acetylat\n Location: Nucleus\n\nProtein 2: IL12RB2 (Q99665)\n Function: Receptor for interleukin-12. This subunit is the signaling component coupling to the JAK2/STAT4 pathway. Promotes the proliferation of T-cells as well as NK cells. Induces the promotion of T-cells tow\n Location: Membrane\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that USP22 and IL12RB2 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1427336, "gene_symbol_1": "USP22", "gene_symbol_2": "IL12RB2", "detection_method": null}} +{"question_id": "PPIL1-0689", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: TP53 (P04637)\n Function: Multifunctional transcription factor that induces cell cycle arrest, DNA repair or apoptosis upon binding to its target DNA sequence (PubMed:11025664, PubMed:12524540, PubMed:12810724, PubMed:15186775\n Location: Cytoplasm\n\nProtein 2: NUBP1 (P53384)\n Function: Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery (PubMed:18573874). Required for maturation of extramitochondrial Fe-S proteins (PubMed:18573874). The NUBP1-NUBP2 heterot\n Location: Cytoplasm\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that TP53 and NUBP1 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1620800, "gene_symbol_1": "TP53", "gene_symbol_2": "NUBP1", "detection_method": null}} +{"question_id": "PPIL1-0690", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: CASP2 (P42575)\n Function: Is a regulator of the cascade of caspases responsible for apoptosis execution (PubMed:11156409, PubMed:15073321, PubMed:8087842). Might function by either activating some proteins required for cell de\n\nProtein 2: VPS11 (Q9H270)\n Function: Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act as a core component of the putative H\n Location: Endosome\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that CASP2 and VPS11 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1050493, "gene_symbol_1": "CASP2", "gene_symbol_2": "VPS11", "detection_method": null}} +{"question_id": "PPIL1-0691", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: TFPT (P0C1Z6)\n Function: Appears to promote apoptosis in a p53/TP53-independent manner\n Location: Nucleus\n\nProtein 2: AP3S2 (P59780)\n Function: Part of the AP-3 complex, an adaptor-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding\n Location: Golgi apparatus\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that TFPT and AP3S2 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 672912, "gene_symbol_1": "TFPT", "gene_symbol_2": "AP3S2", "detection_method": null}} +{"question_id": "PPIL1-0692", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: BCL2L11 (O43521)\n Function: Induces apoptosis and anoikis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than isoform BimEL, \n Location: Endomembrane system\n\nProtein 2: SMARCA4 (P51532)\n Function: ATPase involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that c\n Location: Nucleus\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that BCL2L11 and SMARCA4 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 692190, "gene_symbol_1": "BCL2L11", "gene_symbol_2": "SMARCA4", "detection_method": null}} +{"question_id": "PPIL1-0693", "task": "ppi-l1", "split": "val", "difficulty": "hard", "context_text": "Protein 1: DNMT3L (Q9UJW3)\n Function: Catalytically inactive regulatory factor of DNA methyltransferases that can either promote or inhibit DNA methylation depending on the context (By similarity). Essential for the function of DNMT3A and\n Location: Nucleus\n\nProtein 2: INTS8 (Q75QN2)\n Function: Component of the integrator complex, a multiprotein complex that terminates RNA polymerase II (Pol II) transcription in the promoter-proximal region of genes (PubMed:28542170, PubMed:33243860, PubMed:\n Location: Nucleus\n\nEvidence: Analysis of protein complex data indicates DNMT3L and INTS8 are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 855281, "gene_symbol_1": "DNMT3L", "gene_symbol_2": "INTS8", "detection_method": null}} +{"question_id": "PPIL1-0694", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: ATP6V0D1 (P61421)\n Function: Subunit of the V0 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates prot\n Location: Membrane\n\nProtein 2: CYBA (P13498)\n Function: Subunit of NADPH oxidase complexes that is required for the NADPH oxidase activity that generates, in various cell types, superoxide from molecular oxygen utilizing NADPH as an electron donor (PubMed:\n Location: Cell membrane\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that ATP6V0D1 and CYBA do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 582005, "gene_symbol_1": "ATP6V0D1", "gene_symbol_2": "CYBA", "detection_method": null}} +{"question_id": "PPIL1-0695", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: ACTL6A (O96019)\n Function: Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry ou\n Location: Nucleus\n\nProtein 2: GATAD2A (Q86YP4)\n Function: Transcriptional repressor (PubMed:12183469, PubMed:16415179). Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin (PubMed:16428440, PubMed:289\n Location: Nucleus speckle\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that ACTL6A and GATAD2A co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1130039, "gene_symbol_1": "ACTL6A", "gene_symbol_2": "GATAD2A", "detection_method": null}} +{"question_id": "PPIL1-0696", "task": "ppi-l1", "split": "val", "difficulty": "hard", "context_text": "Protein 1: VPS4B (O75351)\n Function: Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their ATP-dependent disassembly, possibly in combination \n Location: Late endosome membrane\n\nProtein 2: CHCHD3 (Q9NX63)\n Function: Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation \n Location: Mitochondrion inner membrane\n\nEvidence: Analysis of protein complex data indicates VPS4B and CHCHD3 are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1584543, "gene_symbol_1": "VPS4B", "gene_symbol_2": "CHCHD3", "detection_method": null}} +{"question_id": "PPIL1-0697", "task": "ppi-l1", "split": "val", "difficulty": "hard", "context_text": "Protein 1: CDK5R1 (Q15078)\n Function: p35 is a neuron specific activator of CDK5. The complex p35/CDK5 is required for neurite outgrowth and cortical lamination. Involved in dendritic spine morphogenesis by mediating the EFNA1-EPHA4 signa\n Location: Cell membrane\n\nProtein 2: RBBP7 (Q16576)\n Function: Core histone-binding subunit that may target chromatin remodeling factors, histone acetyltransferases and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal \n Location: Nucleus\n\nEvidence: Analysis of protein complex data indicates CDK5R1 and RBBP7 are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1124798, "gene_symbol_1": "CDK5R1", "gene_symbol_2": "RBBP7", "detection_method": null}} +{"question_id": "PPIL1-0698", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: KCNJ11 (Q14654)\n Function: Inward rectifier potassium channel that forms the pore of ATP-sensitive potassium channels (KATP), regulating potassium permeability as a function of cytoplasmic ATP and ADP concentrations in many dif\n Location: Membrane\n\nProtein 2: INTS10 (Q9NVR2)\n Function: Component of the integrator complex, a multiprotein complex that terminates RNA polymerase II (Pol II) transcription in the promoter-proximal region of genes (PubMed:38570683, PubMed:38823386). The in\n Location: Nucleus\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that KCNJ11 and INTS10 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 907657, "gene_symbol_1": "KCNJ11", "gene_symbol_2": "INTS10", "detection_method": null}} +{"question_id": "PPIL1-0699", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: KAT14 (Q9H8E8)\n Function: Component of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4. May function as a scaffold for the ATAC complex to promote ATAC complex stability. Has also weak\n Location: Nucleus\n\nProtein 2: C4A (P0C0L4)\n Function: Precursor of non-enzymatic components of the classical, lectin and GZMK complement pathways, which consist in a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling \n Location: Secreted\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that KAT14 and C4A co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 943130, "gene_symbol_1": "KAT14", "gene_symbol_2": "C4A", "detection_method": null}} +{"question_id": "PPIL1-0700", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: INTS13 (Q9NVM9)\n Function: Component of the integrator complex, a multiprotein complex that terminates RNA polymerase II (Pol II) transcription in the promoter-proximal region of genes (PubMed:38570683, PubMed:38823386). The in\n Location: Nucleus\n\nProtein 2: TGFBRAP1 (Q8WUH2)\n Function: Plays a role in the TGF-beta/activin signaling pathway. It associates with inactive heteromeric TGF-beta and activin receptor complexes, mainly through the type II receptor, and is released upon activ\n Location: Cytoplasm\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that INTS13 and TGFBRAP1 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1077517, "gene_symbol_1": "INTS13", "gene_symbol_2": "TGFBRAP1", "detection_method": null}} +{"question_id": "PPIL1-0701", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: DAG1 (Q14118)\n Function: The dystroglycan complex is involved in a number of signaling events and processes including laminin deposition and extracellular matrix assembly, acetylcholine receptor clustering, sarcolemmal stabil\n Location: Secreted, extracellular space\n\nProtein 2: LSM8 (O95777)\n Function: Plays a role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex) (Pub\n Location: Nucleus\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that DAG1 and LSM8 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1221112, "gene_symbol_1": "DAG1", "gene_symbol_2": "LSM8", "detection_method": null}} +{"question_id": "PPIL1-0702", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: DPM1 (O60762)\n Function: Transfers mannose from GDP-mannose to dolichol monophosphate to form dolichol phosphate mannose (Dol-P-Man) which is the mannosyl donor in pathways leading to N-glycosylation, glycosyl phosphatidylino\n Location: Endoplasmic reticulum\n\nProtein 2: TOMM22 (Q9NS69)\n Function: Central receptor component of the translocase of the outer membrane of mitochondria (TOM) complex essential for the recognition and translocation of cytosolically synthesized mitochondrial preproteins\n Location: Mitochondrion outer membrane\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that DPM1 and TOMM22 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1217467, "gene_symbol_1": "DPM1", "gene_symbol_2": "TOMM22", "detection_method": null}} +{"question_id": "PPIL1-0703", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: CIAO2A (Q9H5X1)\n Function: Component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into extramitochondrial Fe/S proteins (PubMed:23891\n Location: Cytoplasm\n\nProtein 2: TRMT10C (Q7L0Y3)\n Function: Mitochondrial tRNA N(1)-methyltransferase involved in mitochondrial tRNA maturation (PubMed:18984158, PubMed:21593607, PubMed:23042678, PubMed:27132592). Component of mitochondrial ribonuclease P, a c\n Location: Mitochondrion matrix, mitochondrion nucleoid\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that CIAO2A and TRMT10C do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1002764, "gene_symbol_1": "CIAO2A", "gene_symbol_2": "TRMT10C", "detection_method": null}} +{"question_id": "PPIL1-0704", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: H2AC4 (P04908)\n Function: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central r\n Location: Nucleus\n\nProtein 2: EDC3 (Q96F86)\n Function: Binds single-stranded RNA. Involved in the process of mRNA degradation and in the positive regulation of mRNA decapping. May play a role in spermiogenesis and oogenesis\n Location: Cytoplasm, P-body\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that H2AC4 and EDC3 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1031728, "gene_symbol_1": "H2AC4", "gene_symbol_2": "EDC3", "detection_method": null}} +{"question_id": "PPIL1-0705", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: GTF2A2 (P52657)\n Function: TFIIA is a component of the transcription machinery of RNA polymerase II and plays an important role in transcriptional activation. TFIIA in a complex with TBP mediates transcriptional activity\n Location: Nucleus\n\nProtein 2: FAAP100 (Q0VG06)\n Function: Plays a role in Fanconi anemia-associated DNA damage response network. Regulates FANCD2 monoubiquitination and the stability of the FA core complex. Induces chromosomal instability as well as hypersen\n Location: Nucleus\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that GTF2A2 and FAAP100 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 771838, "gene_symbol_1": "GTF2A2", "gene_symbol_2": "FAAP100", "detection_method": null}} +{"question_id": "PPIL1-0706", "task": "ppi-l1", "split": "val", "difficulty": "hard", "context_text": "Protein 1: CAMLG (P49069)\n Function: Required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum (PubMed:23041287, PubMed:24392163, PubMed:27226539). Together with GET1/WRB, acts as a membrane\n Location: Endoplasmic reticulum membrane\n\nProtein 2: ITGA6 (P23229)\n Function: Integrin alpha-6/beta-1 (ITGA6:ITGB1) is a receptor for laminin on platelets (By similarity). Integrin alpha-6/beta-1 (ITGA6:ITGB1) is present in oocytes and is involved in sperm-egg fusion (By simila\n Location: Cell membrane\n\nEvidence: Analysis of protein complex data indicates CAMLG and ITGA6 are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 938424, "gene_symbol_1": "CAMLG", "gene_symbol_2": "ITGA6", "detection_method": null}} +{"question_id": "PPIL1-0707", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: SS18 (Q15532)\n Function: Appears to function synergistically with RBM14 as a transcriptional coactivator. Isoform 1 and isoform 2 function in nuclear receptor coactivation. Isoform 1 and isoform 2 function in general transcri\n Location: Nucleus\n\nProtein 2: SNUPN (O95149)\n Function: Functions as an U snRNP-specific nuclear import adapter. Involved in the trimethylguanosine (m3G)-cap-dependent nuclear import of U snRNPs. Binds specifically to the terminal m3G-cap U snRNAs\n Location: Nucleus\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that SS18 and SNUPN co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1687787, "gene_symbol_1": "SS18", "gene_symbol_2": "SNUPN", "detection_method": null}} +{"question_id": "PPIL1-0708", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: NELFE (P18615)\n Function: Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II (PubMed:10199401, PubMed:27256882). The NELF complex, which acts via a\n Location: Nucleus\n\nProtein 2: ARID1B (Q8NFD5)\n Function: Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry ou\n Location: Nucleus\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that NELFE and ARID1B do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 668349, "gene_symbol_1": "NELFE", "gene_symbol_2": "ARID1B", "detection_method": null}} +{"question_id": "PPIL1-0709", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: COG2 (Q14746)\n Function: Required for normal Golgi morphology and function\n Location: Golgi apparatus membrane\n\nProtein 2: MRPS34 (P82930)\n Function: Required for mitochondrial translation, plays a role in maintaining the stability of the small ribosomal subunit and the 12S rRNA that are required for mitoribosome formation\n Location: Mitochondrion\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that COG2 and MRPS34 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1357405, "gene_symbol_1": "COG2", "gene_symbol_2": "MRPS34", "detection_method": null}} +{"question_id": "PPIL1-0710", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: COX4I1 (P13073)\n Function: Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes suc\n Location: Mitochondrion inner membrane\n\nProtein 2: CCNH (P51946)\n Function: Regulates CDK7, the catalytic subunit of the CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK comp\n Location: Nucleus\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that COX4I1 and CCNH co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1160249, "gene_symbol_1": "COX4I1", "gene_symbol_2": "CCNH", "detection_method": null}} +{"question_id": "PPIL1-0711", "task": "ppi-l1", "split": "val", "difficulty": "hard", "context_text": "Protein 1: CSNK2A1 (P68400)\n Function: Catalytic subunit of a constitutively active serine/threonine-protein kinase complex that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine\n Location: Nucleus\n\nProtein 2: CHAF1A (Q13111)\n Function: Acts as a component of the histone chaperone complex chromatin assembly factor 1 (CAF-1), which assembles histone octamers onto DNA during replication and repair. CAF-1 performs the first step of the \n Location: Nucleus\n\nEvidence: Analysis of protein complex data indicates CSNK2A1 and CHAF1A are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 763528, "gene_symbol_1": "CSNK2A1", "gene_symbol_2": "CHAF1A", "detection_method": null}} +{"question_id": "PPIL1-0712", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: CD3G (P09693)\n Function: Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-medi\n Location: Cell membrane\n\nProtein 2: SOS1 (Q07889)\n Function: Promotes the exchange of Ras-bound GDP by GTP (PubMed:8493579). Probably by promoting Ras activation, regulates phosphorylation of MAP kinase MAPK3/ERK1 in response to EGF (PubMed:17339331). Catalytic\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that CD3G and SOS1 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 539823, "gene_symbol_1": "CD3G", "gene_symbol_2": "SOS1", "detection_method": null}} +{"question_id": "PPIL1-0713", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: NR1H3 (Q13133)\n Function: Nuclear receptor that exhibits a ligand-dependent transcriptional activation activity (PubMed:19481530, PubMed:25661920, PubMed:37478846). Interaction with retinoic acid receptor (RXR) shifts RXR from\n Location: Nucleus\n\nProtein 2: CCNA2 (P20248)\n Function: Cyclin which controls both the G1/S and the G2/M transition phases of the cell cycle. Functions through the formation of specific serine/threonine protein kinase holoenzyme complexes with the cyclin-d\n Location: Nucleus\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that NR1H3 and CCNA2 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1713699, "gene_symbol_1": "NR1H3", "gene_symbol_2": "CCNA2", "detection_method": null}} +{"question_id": "PPIL1-0714", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: ATP11B (Q9Y2G3)\n Function: Catalytic component of a P4-ATPase flippase complex which catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids, phosphatidylserines (PS) and phosphatidylethanolamines (PE), f\n Location: Recycling endosome membrane\n\nProtein 2: ITGB6 (P18564)\n Function: Integrin alpha-V:beta-6 (ITGAV:ITGB6) is a receptor for fibronectin and cytotactin (PubMed:17158881, PubMed:17545607). It recognizes the sequence R-G-D in its ligands (PubMed:17158881, PubMed:17545607\n Location: Cell membrane\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that ATP11B and ITGB6 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 779074, "gene_symbol_1": "ATP11B", "gene_symbol_2": "ITGB6", "detection_method": null}} +{"question_id": "PPIL1-0715", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: CHRAC1 (Q9NRG0)\n Function: Forms a complex with DNA polymerase epsilon subunit POLE3 and binds naked DNA, which is then incorporated into chromatin, aided by the nucleosome remodeling activity of ISWI/SNF2H and ACF1. Does not e\n Location: Nucleus\n\nProtein 2: INTS7 (Q9NVH2)\n Function: Component of the integrator complex, a multiprotein complex that terminates RNA polymerase II (Pol II) transcription in the promoter-proximal region of genes (PubMed:33243860, PubMed:38570683). The in\n Location: Nucleus\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that CHRAC1 and INTS7 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1009942, "gene_symbol_1": "CHRAC1", "gene_symbol_2": "INTS7", "detection_method": null}} +{"question_id": "PPIL1-0716", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: CLUAP1 (Q96AJ1)\n Function: Required for cilia biogenesis. Appears to function within the multiple intraflagellar transport complex B (IFT-B). Key regulator of hedgehog signaling\n Location: Cell projection, cilium\n\nProtein 2: IGHA1 (P01876)\n Function: Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral i\n Location: Secreted\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that CLUAP1 and IGHA1 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1419144, "gene_symbol_1": "CLUAP1", "gene_symbol_2": "IGHA1", "detection_method": null}} +{"question_id": "PPIL1-0717", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: SMCR8 (Q8TEV9)\n Function: Component of the C9orf72-SMCR8 complex, a complex that has guanine nucleotide exchange factor (GEF) activity and regulates autophagy (PubMed:20562859, PubMed:27103069, PubMed:27193190, PubMed:27559131\n Location: Cytoplasm\n\nProtein 2: FANCD2 (Q9BXW9)\n Function: Required for maintenance of chromosomal stability (PubMed:11239453, PubMed:14517836). Promotes accurate and efficient pairing of homologs during meiosis (PubMed:14517836). Involved in the repair of DN\n Location: Nucleus\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that SMCR8 and FANCD2 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1389660, "gene_symbol_1": "SMCR8", "gene_symbol_2": "FANCD2", "detection_method": null}} +{"question_id": "PPIL1-0718", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: BID (P55957)\n Function: Induces caspases and apoptosis (PubMed:14583606). Counters the protective effect of BCL2 (By similarity)\n Location: Cytoplasm\n\nProtein 2: UXT (Q9UBK9)\n Function: Involved in gene transcription regulation (PubMed:21730289, PubMed:28106301). Acts in concert with the corepressor URI1 to regulate androgen receptor AR-mediated transcription (PubMed:11854421, PubMed\n Location: Cytoplasm\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that BID and UXT co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1695958, "gene_symbol_1": "BID", "gene_symbol_2": "UXT", "detection_method": null}} +{"question_id": "PPIL1-0719", "task": "ppi-l1", "split": "val", "difficulty": "hard", "context_text": "Protein 1: CLUAP1 (Q96AJ1)\n Function: Required for cilia biogenesis. Appears to function within the multiple intraflagellar transport complex B (IFT-B). Key regulator of hedgehog signaling\n Location: Cell projection, cilium\n\nProtein 2: SNUPN (O95149)\n Function: Functions as an U snRNP-specific nuclear import adapter. Involved in the trimethylguanosine (m3G)-cap-dependent nuclear import of U snRNPs. Binds specifically to the terminal m3G-cap U snRNAs\n Location: Nucleus\n\nEvidence: Analysis of protein complex data indicates CLUAP1 and SNUPN are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1294182, "gene_symbol_1": "CLUAP1", "gene_symbol_2": "SNUPN", "detection_method": null}} +{"question_id": "PPIL1-0720", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: SUCLG1 (P53597)\n Function: Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of either ATP or GTP and thus represents the only step of substrate-level pho\n Location: Mitochondrion\n\nProtein 2: TAF8 (Q7Z7C8)\n Function: The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription (PubMed:33795473). TFIID recognizes and binds promoters with or \n Location: Nucleus\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that SUCLG1 and TAF8 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1247820, "gene_symbol_1": "SUCLG1", "gene_symbol_2": "TAF8", "detection_method": null}} +{"question_id": "PPIL1-0721", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: BCAS2 (O75934)\n Function: Required for pre-mRNA splicing as component of the activated spliceosome (PubMed:28076346, PubMed:28502770, PubMed:29301961, PubMed:29360106, PubMed:30705154). Component of the PRP19-CDC5L complex tha\n Location: Nucleus\n\nProtein 2: LAMB2 (P55268)\n Function: Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other ext\n Location: Secreted, extracellular space, extracellular matrix, basement membrane\n\nEvidence: Analysis of protein complex data indicates BCAS2 and LAMB2 are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 621788, "gene_symbol_1": "BCAS2", "gene_symbol_2": "LAMB2", "detection_method": null}} +{"question_id": "PPIL1-0722", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: ACTB (P60709)\n Function: Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed:25255767, PubMed:29581253). Actin exists in both monomeric (\n Location: Cytoplasm, cytoskeleton\n\nProtein 2: ATP4A (P20648)\n Function: The catalytic subunit of the gastric H(+)/K(+) ATPase pump which transports H(+) ions in exchange for K(+) ions across the apical membrane of parietal cells. Uses ATP as an energy source to pump H(+) \n Location: Apical cell membrane\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that ACTB and ATP4A co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1249091, "gene_symbol_1": "ACTB", "gene_symbol_2": "ATP4A", "detection_method": null}} +{"question_id": "PPIL1-0723", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: VPS37A (Q8NEZ2)\n Function: Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies. May be involved in cell growth an\n Location: Late endosome membrane\n\nProtein 2: KANSL3 (Q9P2N6)\n Function: Non-catalytic component of the NSL histone acetyltransferase complex, a multiprotein complex that mediates histone H4 acetylation at 'Lys-5'- and 'Lys-8' (H4K5ac and H4K8ac) at transcription start sit\n Location: Nucleus\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that VPS37A and KANSL3 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 620389, "gene_symbol_1": "VPS37A", "gene_symbol_2": "KANSL3", "detection_method": null}} +{"question_id": "PPIL1-0724", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: TIMM8A (O60220)\n Function: Mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of b\n Location: Mitochondrion inner membrane\n\nProtein 2: RAD23B (P54727)\n Function: Multifunctional protein that participates in histone H4K20 demethylation, DNA repair, ubiquitin-dependent protein degradation and transcriptional regulation (PubMed:10488153, PubMed:32209475, PubMed:9\n Location: Nucleus\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that TIMM8A and RAD23B co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 753973, "gene_symbol_1": "TIMM8A", "gene_symbol_2": "RAD23B", "detection_method": null}} +{"question_id": "PPIL1-0725", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: YEATS4 (O95619)\n Function: Chromatin reader component of the NuA4 histone acetyltransferase (HAT) complex, a complex involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 a\n Location: Nucleus\n\nProtein 2: SNRPD3 (P62318)\n Function: Plays a role in pre-mRNA splicing as a core component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome (PubMed:11991638, PubMed:1\n Location: Cytoplasm, cytosol\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that YEATS4 and SNRPD3 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 683343, "gene_symbol_1": "YEATS4", "gene_symbol_2": "SNRPD3", "detection_method": null}} +{"question_id": "PPIL1-0726", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: PATJ (Q8NI35)\n Function: Scaffolding protein that facilitates the localization of proteins to the cell membrane (PubMed:11927608, PubMed:16678097, PubMed:22006950). Required for the correct formation of tight junctions and ep\n Location: Cell junction, tight junction\n\nProtein 2: SUPT3H (O75486)\n Function: Probable transcriptional activator\n Location: Nucleus\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that PATJ and SUPT3H co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 595116, "gene_symbol_1": "PATJ", "gene_symbol_2": "SUPT3H", "detection_method": null}} +{"question_id": "PPIL1-0727", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: HAUS3 (Q68CZ6)\n Function: Contributes to mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex\n Location: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome\n\nProtein 2: CACNA1C (Q13936)\n Function: Pore-forming, alpha-1C subunit of the voltage-gated calcium channel that gives rise to L-type calcium currents (PubMed:12181424, PubMed:15454078, PubMed:15863612, PubMed:16299511, PubMed:17224476, Pub\n Location: Cell membrane\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that HAUS3 and CACNA1C co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 707236, "gene_symbol_1": "HAUS3", "gene_symbol_2": "CACNA1C", "detection_method": null}} +{"question_id": "PPIL1-0728", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: PFDN6 (O15212)\n Function: Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing path\n\nProtein 2: UQCRB (P14927)\n Function: Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation. The resp\n Location: Mitochondrion inner membrane\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that PFDN6 and UQCRB co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1390640, "gene_symbol_1": "PFDN6", "gene_symbol_2": "UQCRB", "detection_method": null}} +{"question_id": "PPIL1-0729", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: TAF13 (Q15543)\n Function: The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription (PubMed:33795473, PubMed:9695952). TFIID recognizes and binds pr\n Location: Nucleus\n\nProtein 2: JDP2 (Q8WYK2)\n Function: Component of the AP-1 transcription factor that represses transactivation mediated by the Jun family of proteins. Involved in a variety of transcriptional responses associated with AP-1 such as UV-ind\n Location: Nucleus\n\nEvidence: Analysis of protein complex data indicates TAF13 and JDP2 are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1721200, "gene_symbol_1": "TAF13", "gene_symbol_2": "JDP2", "detection_method": null}} +{"question_id": "PPIL1-0730", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: CENPI (Q92674)\n Function: Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be\n Location: Nucleus\n\nProtein 2: IGHG4 (P01861)\n Function: Constant region of immunoglobulin (Ig) heavy chains. Igs are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound Igs se\n Location: Secreted\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that CENPI and IGHG4 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1696363, "gene_symbol_1": "CENPI", "gene_symbol_2": "IGHG4", "detection_method": null}} +{"question_id": "PPIL1-0731", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: FANCE (Q9HB96)\n Function: As part of the Fanconi anemia (FA) complex functions in DNA cross-links repair. Required for the nuclear accumulation of FANCC and provides a critical bridge between the FA complex and FANCD2\n Location: Nucleus\n\nProtein 2: MED13 (Q9UHV7)\n Function: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene\n Location: Nucleus\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that FANCE and MED13 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 895213, "gene_symbol_1": "FANCE", "gene_symbol_2": "MED13", "detection_method": null}} +{"question_id": "PPIL1-0732", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: PPP4C (P60510)\n Function: Protein phosphatase that is involved in many processes such as microtubule organization at centrosomes, maturation of spliceosomal snRNPs, apoptosis, DNA repair, tumor necrosis factor (TNF)-alpha sign\n Location: Cytoplasm\n\nProtein 2: IFT25 (Q9Y547)\n Function: Component of the IFT complex B required for sonic hedgehog/SHH signaling. May mediate transport of SHH components: required for the export of SMO and PTCH1 receptors out of the cilium and the accumula\n Location: Cell projection, cilium\n\nEvidence: Analysis of protein complex data indicates PPP4C and IFT25 are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1705637, "gene_symbol_1": "PPP4C", "gene_symbol_2": "IFT25", "detection_method": null}} +{"question_id": "PPIL1-0733", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: PPP3R1 (P63098)\n Function: Regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity\n Location: Cytoplasm, cytosol\n\nProtein 2: HPS6 (Q86YV9)\n Function: May regulate the synthesis and function of lysosomes and of highly specialized organelles, such as melanosomes and platelet dense granules (PubMed:17041891). Acts as a cargo adapter for the dynein-dyn\n Location: Microsome membrane\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that PPP3R1 and HPS6 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 671666, "gene_symbol_1": "PPP3R1", "gene_symbol_2": "HPS6", "detection_method": null}} +{"question_id": "PPIL1-0734", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: SDHD (O14521)\n Function: Membrane-anchoring subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to\n Location: Mitochondrion inner membrane\n\nProtein 2: INO80B (Q9C086)\n Function: Induces growth and cell cycle arrests at the G1 phase of the cell cycle\n Location: Nucleus\n\nEvidence: Analysis of protein complex data indicates SDHD and INO80B are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 750177, "gene_symbol_1": "SDHD", "gene_symbol_2": "INO80B", "detection_method": null}} +{"question_id": "PPIL1-0735", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: LAMB1 (P07942)\n Function: Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other ext\n Location: Secreted, extracellular space, extracellular matrix, basement membrane\n\nProtein 2: RELA (Q04206)\n Function: NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to\n Location: Nucleus\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that LAMB1 and RELA co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1312031, "gene_symbol_1": "LAMB1", "gene_symbol_2": "RELA", "detection_method": null}} +{"question_id": "PPIL1-0736", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: CHRNB2 (P17787)\n Function: Component of neuronal acetylcholine receptors (nAChRs) that function as pentameric, ligand-gated cation channels with high calcium permeability among other activities. nAChRs are excitatory neurotrasn\n Location: Synaptic cell membrane\n\nProtein 2: ATP1B1 (P05026)\n Function: This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The beta subunit regulates,\n Location: Cell membrane\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that CHRNB2 and ATP1B1 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1222402, "gene_symbol_1": "CHRNB2", "gene_symbol_2": "ATP1B1", "detection_method": null}} +{"question_id": "PPIL1-0737", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: TP53 (P04637)\n Function: Multifunctional transcription factor that induces cell cycle arrest, DNA repair or apoptosis upon binding to its target DNA sequence (PubMed:11025664, PubMed:12524540, PubMed:12810724, PubMed:15186775\n Location: Cytoplasm\n\nProtein 2: IFT25 (Q9Y547)\n Function: Component of the IFT complex B required for sonic hedgehog/SHH signaling. May mediate transport of SHH components: required for the export of SMO and PTCH1 receptors out of the cilium and the accumula\n Location: Cell projection, cilium\n\nEvidence: Analysis of protein complex data indicates TP53 and IFT25 are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1696245, "gene_symbol_1": "TP53", "gene_symbol_2": "IFT25", "detection_method": null}} +{"question_id": "PPIL1-0738", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: INTS11 (Q5TA45)\n Function: RNA endonuclease component of the integrator complex, a multiprotein complex that terminates RNA polymerase II (Pol II) transcription in the promoter-proximal region of genes (PubMed:16239144, PubMed:\n Location: Nucleus\n\nProtein 2: CDC5L (Q99459)\n Function: DNA-binding protein involved in cell cycle control. May act as a transcription activator. Plays a role in pre-mRNA splicing as core component of precatalytic, catalytic and postcatalytic spliceosomal \n Location: Nucleus\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that INTS11 and CDC5L co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 627920, "gene_symbol_1": "INTS11", "gene_symbol_2": "CDC5L", "detection_method": null}} +{"question_id": "PPIL1-0739", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: TERF1 (P54274)\n Function: Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and negatively regulates telomere length (PubMed:31595153). Involved in the regulation of the mitotic spindle. Component of the shelterin comple\n Location: Nucleus\n\nProtein 2: SUPT7L (O94864)\n Location: Nucleus\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that TERF1 and SUPT7L do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1038842, "gene_symbol_1": "TERF1", "gene_symbol_2": "SUPT7L", "detection_method": null}} +{"question_id": "PPIL1-0740", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: KRT82 (Q9NSB4)\n\nProtein 2: NFE2L3 (Q9Y4A8)\n Function: Activates erythroid-specific, globin gene expression\n Location: Nucleus\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that KRT82 and NFE2L3 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 605202, "gene_symbol_1": "KRT82", "gene_symbol_2": "NFE2L3", "detection_method": null}} +{"question_id": "PPIL1-0741", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: NAA20 (P61599)\n Function: Catalytic subunit of the NatB complex which catalyzes acetylation of the N-terminal methionine residues of peptides beginning with Met-Asp, Met-Glu, Met-Asn and Met-Gln (PubMed:34230638). Proteins wit\n Location: Cytoplasm\n\nProtein 2: TAF5 (Q15542)\n Function: The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription (PubMed:33795473). TFIID recognizes and binds promoters with or \n Location: Nucleus\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that NAA20 and TAF5 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1629195, "gene_symbol_1": "NAA20", "gene_symbol_2": "TAF5", "detection_method": null}} +{"question_id": "PPIL1-0742", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: PFDN6 (O15212)\n Function: Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing path\n\nProtein 2: EXOSC7 (Q15024)\n Function: Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA \n Location: Nucleus, nucleolus\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that PFDN6 and EXOSC7 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1174424, "gene_symbol_1": "PFDN6", "gene_symbol_2": "EXOSC7", "detection_method": null}} +{"question_id": "PPIL1-0743", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: TADA3 (O75528)\n Function: Functions as a component of the PCAF complex. The PCAF complex is capable of efficiently acetylating histones in a nucleosomal context. The PCAF complex could be considered as the human version of the\n Location: Nucleus\n\nProtein 2: CRB1 (P82279)\n Function: Plays a role in photoreceptor morphogenesis in the retina (By similarity). May maintain cell polarization and adhesion (By similarity)\n Location: Apical cell membrane\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that TADA3 and CRB1 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1064506, "gene_symbol_1": "TADA3", "gene_symbol_2": "CRB1", "detection_method": null}} +{"question_id": "PPIL1-0744", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: NELFA (Q9H3P2)\n Function: Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex\n Location: Nucleus\n\nProtein 2: IGLC7 (A0M8Q6)\n Function: Constant region of immunoglobulin light chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral i\n Location: Secreted\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that NELFA and IGLC7 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 969337, "gene_symbol_1": "NELFA", "gene_symbol_2": "IGLC7", "detection_method": null}} +{"question_id": "PPIL1-0745", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: KIF3C (O14782)\n Function: Microtubule-based anterograde translocator for membranous organelles\n Location: Cytoplasm, cytoskeleton\n\nProtein 2: SUV39H1 (O43463)\n Function: Histone methyltransferase that specifically mediates trimethylation of 'Lys-9' of histone H3 (H3K9me3) using monomethylated H3 'Lys-9' (H3K9me1) as substrate (PubMed:10949293, PubMed:11242053, PubMed:\n Location: Nucleus\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that KIF3C and SUV39H1 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 785437, "gene_symbol_1": "KIF3C", "gene_symbol_2": "SUV39H1", "detection_method": null}} +{"question_id": "PPIL1-0746", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: COX8A (P10176)\n Function: Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes suc\n Location: Mitochondrion inner membrane\n\nProtein 2: SF3B1 (O75533)\n Function: Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs (PubMed:12234937, PubMed:27720643, PubMed:32494006, PubMed:3\n Location: Nucleus\n\nEvidence: Analysis of protein complex data indicates COX8A and SF3B1 are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1425782, "gene_symbol_1": "COX8A", "gene_symbol_2": "SF3B1", "detection_method": null}} +{"question_id": "PPIL1-0747", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: SNRPA1 (P09661)\n Function: Involved in pre-mRNA splicing as component of the spliceosome (PubMed:11991638, PubMed:27035939, PubMed:28076346, PubMed:28502770, PubMed:28781166, PubMed:32494006). Associated with sn-RNP U2, where i\n Location: Nucleus\n\nProtein 2: SGF29 (Q96ES7)\n Function: Chromatin reader component of some histone acetyltransferase (HAT) SAGA-type complexes like the TFTC-HAT, ATAC or STAGA complexes (PubMed:19103755, PubMed:20850016, PubMed:21685874, PubMed:26421618, P\n Location: Nucleus\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that SNRPA1 and SGF29 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 551772, "gene_symbol_1": "SNRPA1", "gene_symbol_2": "SGF29", "detection_method": null}} +{"question_id": "PPIL1-0748", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: AP5M1 (Q9H0R1)\n Function: As part of AP-5, a probable fifth adaptor protein complex it may be involved in endosomal transport. According to PubMed:18395520, it may play a role in cell death\n Location: Cytoplasm, cytosol\n\nProtein 2: DBP (Q10586)\n Function: This transcriptional activator recognizes and binds to the sequence 5'-RTTAYGTAAY-3' found in the promoter of genes such as albumin, CYP2A4 and CYP2A5. It is not essential for circadian rhythm generat\n Location: Nucleus\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that AP5M1 and DBP do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 779419, "gene_symbol_1": "AP5M1", "gene_symbol_2": "DBP", "detection_method": null}} +{"question_id": "PPIL1-0749", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: NFE2L3 (Q9Y4A8)\n Function: Activates erythroid-specific, globin gene expression\n Location: Nucleus\n\nProtein 2: GSPT1 (P15170)\n Function: GTPase component of the eRF1-eRF3-GTP ternary complex, a ternary complex that mediates translation termination in response to the termination codons UAA, UAG and UGA (PubMed:15987998, PubMed:19417105,\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that NFE2L3 and GSPT1 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 982015, "gene_symbol_1": "NFE2L3", "gene_symbol_2": "GSPT1", "detection_method": null}} +{"question_id": "PPIL1-0750", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: CXXC1 (Q9P0U4)\n Function: Transcriptional activator that exhibits a unique DNA binding specificity for CpG unmethylated motifs with a preference for CpGG\n Location: Nucleus speckle\n\nProtein 2: TAF3 (Q5VWG9)\n Function: The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription (PubMed:33795473). TFIID recognizes and binds promoters with or \n Location: Nucleus\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that CXXC1 and TAF3 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1210915, "gene_symbol_1": "CXXC1", "gene_symbol_2": "TAF3", "detection_method": null}} +{"question_id": "PPIL1-0751", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: NUDT21 (O43809)\n Function: Component of the cleavage factor Im (CFIm) complex that functions as an activator of the pre-mRNA 3'-end cleavage and polyadenylation processing required for the maturation of pre-mRNA into functional\n Location: Nucleus\n\nProtein 2: POLA1 (P09884)\n Function: Catalytic subunit of the DNA polymerase alpha complex (also known as the alpha DNA polymerase-primase complex) which plays an essential role in the initiation of DNA synthesis. During the S phase of t\n Location: Nucleus\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that NUDT21 and POLA1 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1387875, "gene_symbol_1": "NUDT21", "gene_symbol_2": "POLA1", "detection_method": null}} +{"question_id": "PPIL1-0752", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: TNNI3 (P19429)\n Function: Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity\n\nProtein 2: RELA (Q04206)\n Function: NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to\n Location: Nucleus\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that TNNI3 and RELA do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1423944, "gene_symbol_1": "TNNI3", "gene_symbol_2": "RELA", "detection_method": null}} +{"question_id": "PPIL1-0753", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: PRKAG3 (Q9UGI9)\n Function: AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism (PubMed:14722619, PubMed:17878938, PubMed\n\nProtein 2: NUS1 (Q96E22)\n Function: With DHDDS, forms the dehydrodolichyl diphosphate synthase (DDS) complex, an essential component of the dolichol monophosphate (Dol-P) biosynthetic machinery (PubMed:21572394, PubMed:25066056, PubMed:\n Location: Endoplasmic reticulum membrane\n\nEvidence: Analysis of protein complex data indicates PRKAG3 and NUS1 are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1400499, "gene_symbol_1": "PRKAG3", "gene_symbol_2": "NUS1", "detection_method": null}} +{"question_id": "PPIL1-0754", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: C1QB (P02746)\n Function: Core component of the complement C1 complex, a multiprotein complex that initiates the classical pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pat\n Location: Secreted\n\nProtein 2: STRADB (Q9C0K7)\n Function: Pseudokinase which, in complex with CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta), binds to and activates STK11/LKB1. Adopts a closed conformation typical of active protein kinases and binds STK11/L\n Location: Nucleus\n\nEvidence: Analysis of protein complex data indicates C1QB and STRADB are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 519436, "gene_symbol_1": "C1QB", "gene_symbol_2": "STRADB", "detection_method": null}} +{"question_id": "PPIL1-0755", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: SMARCE1 (Q969G3)\n Function: Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry ou\n Location: Nucleus\n\nProtein 2: PRKAG3 (Q9UGI9)\n Function: AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism (PubMed:14722619, PubMed:17878938, PubMed\n\nEvidence: Analysis of protein complex data indicates SMARCE1 and PRKAG3 are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 865538, "gene_symbol_1": "SMARCE1", "gene_symbol_2": "PRKAG3", "detection_method": null}} +{"question_id": "PPIL1-0756", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: SNRPA (P09012)\n Function: Component of the spliceosomal U1 snRNP, which is essential for recognition of the pre-mRNA 5' splice-site and the subsequent assembly of the spliceosome. U1 snRNP is the first snRNP to interact with p\n Location: Nucleus\n\nProtein 2: PPP4R1 (Q8TF05)\n Function: Regulatory subunit of serine/threonine-protein phosphatase 4. May play a role in regulation of cell division in renal glomeruli. The PPP4C-PPP4R1 PP4 complex may play a role in dephosphorylation and r\n\nEvidence: Analysis of protein complex data indicates SNRPA and PPP4R1 are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1459618, "gene_symbol_1": "SNRPA", "gene_symbol_2": "PPP4R1", "detection_method": null}} +{"question_id": "PPIL1-0757", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: KRIT1 (O00522)\n Function: Component of the CCM signaling pathway which is a crucial regulator of heart and vessel formation and integrity (By similarity). Negative regulator of angiogenesis. Inhibits endothelial proliferation,\n Location: Cytoplasm, cytoskeleton\n\nProtein 2: HSP90AA1 (P07900)\n Function: Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoe\n Location: Nucleus\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that KRIT1 and HSP90AA1 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 986549, "gene_symbol_1": "KRIT1", "gene_symbol_2": "HSP90AA1", "detection_method": null}} +{"question_id": "PPIL1-0758", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: ATF7 (P17544)\n Function: Stress-responsive chromatin regulator that plays a role in various biological processes including innate immunological memory, adipocyte differentiation or telomerase regulation (PubMed:29490055). In \n Location: Nucleus\n\nProtein 2: INTS12 (Q96CB8)\n Function: Component of the integrator complex, a multiprotein complex that terminates RNA polymerase II (Pol II) transcription in the promoter-proximal region of genes (PubMed:38570683). The integrator complex \n Location: Nucleus\n\nEvidence: Analysis of protein complex data indicates ATF7 and INTS12 are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 882622, "gene_symbol_1": "ATF7", "gene_symbol_2": "INTS12", "detection_method": null}} +{"question_id": "PPIL1-0759", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: SNRPA (P09012)\n Function: Component of the spliceosomal U1 snRNP, which is essential for recognition of the pre-mRNA 5' splice-site and the subsequent assembly of the spliceosome. U1 snRNP is the first snRNP to interact with p\n Location: Nucleus\n\nProtein 2: TMEM186 (Q96B77)\n Function: As part of the MCIA complex, required for efficient assembly of the mitochondrial complex I\n Location: Mitochondrion inner membrane\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that SNRPA and TMEM186 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1546143, "gene_symbol_1": "SNRPA", "gene_symbol_2": "TMEM186", "detection_method": null}} +{"question_id": "PPIL1-0760", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: ITGB2 (P05107)\n Function: Integrin ITGAL:ITGB2 is a receptor for ICAM1, ICAM2 and ICAM3 (PubMed:1676048, PubMed:23775590, PubMed:38195629). Integrin ITGAL:ITGB2 is also a receptor for the secreted form of ubiquitin-like protei\n Location: Cell membrane\n\nProtein 2: CCNK (O75909)\n Function: Regulatory subunit of cyclin-dependent kinases that mediates activation of target kinases. Plays a role in transcriptional regulation via its role in regulating the phosphorylation of the C-terminal d\n Location: Nucleus\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that ITGB2 and CCNK co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 754322, "gene_symbol_1": "ITGB2", "gene_symbol_2": "CCNK", "detection_method": null}} +{"question_id": "PPIL1-0761", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: NAA10 (P41227)\n Function: Catalytic subunit of N-terminal acetyltransferase complexes which display alpha (N-terminal) acetyltransferase activity (PubMed:15496142, PubMed:19420222, PubMed:19826488, PubMed:20145209, PubMed:2015\n Location: Cytoplasm\n\nProtein 2: TRAPPC2L (Q9UL33)\n Function: Plays a role in vesicular transport from endoplasmic reticulum to Golgi\n Location: Cytoplasm, perinuclear region\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that NAA10 and TRAPPC2L co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1191764, "gene_symbol_1": "NAA10", "gene_symbol_2": "TRAPPC2L", "detection_method": null}} +{"question_id": "PPIL1-0762", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: GABRD (O14764)\n Function: Delta subunit of the heteropentameric ligand-gated chloride channel gated by gamma-aminobutyric acid (GABA), a major inhibitory neurotransmitter in the brain (PubMed:35355020). GABA-gated chloride cha\n Location: Cell membrane\n\nProtein 2: SCNN1A (P37088)\n Function: This is one of the three pore-forming subunits of the heterotrimeric epithelial sodium channel (ENaC), a critical regulator of sodium balance and fluid homeostasis (PubMed:30251954, PubMed:32729833, P\n Location: Apical cell membrane\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that GABRD and SCNN1A do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 727593, "gene_symbol_1": "GABRD", "gene_symbol_2": "SCNN1A", "detection_method": null}} +{"question_id": "PPIL1-0763", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: CAPN1 (P07384)\n Function: Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction (PubMed:19617626, PubMed:21531719, PubMed:2\n Location: Cytoplasm\n\nProtein 2: MED12 (Q93074)\n Function: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene\n Location: Nucleus\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that CAPN1 and MED12 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 805266, "gene_symbol_1": "CAPN1", "gene_symbol_2": "MED12", "detection_method": null}} +{"question_id": "PPIL1-0764", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: ITGB7 (P26010)\n Function: Integrin ITGA4/ITGB7 (alpha-4/beta-7) (Peyer patches-specific homing receptor LPAM-1) is an adhesion molecule that mediates lymphocyte migration and homing to gut-associated lymphoid tissue (GALT) (Pr\n Location: Cell membrane\n\nProtein 2: DDOST (P39656)\n Function: Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to a\n Location: Endoplasmic reticulum membrane\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that ITGB7 and DDOST co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 583920, "gene_symbol_1": "ITGB7", "gene_symbol_2": "DDOST", "detection_method": null}} +{"question_id": "PPIL1-0765", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: TTC21B (Q7Z4L5)\n Function: Component of the IFT complex A (IFT-A), a complex required for retrograde ciliary transport and entry into cilia of G protein-coupled receptors (GPCRs). Essential for retrograde trafficking of IFT-1, \n Location: Cytoplasm, cytoskeleton, cilium axoneme\n\nProtein 2: BPTF (Q12830)\n Function: Regulatory subunit of the ATP-dependent NURF-1 and NURF-5 ISWI chromatin remodeling complexes, which form ordered nucleosome arrays on chromatin and facilitate access to DNA during DNA-templated proce\n Location: Cytoplasm\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that TTC21B and BPTF co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1044517, "gene_symbol_1": "TTC21B", "gene_symbol_2": "BPTF", "detection_method": null}} +{"question_id": "PPIL1-0766", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: C1QB (P02746)\n Function: Core component of the complement C1 complex, a multiprotein complex that initiates the classical pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pat\n Location: Secreted\n\nProtein 2: TAPBP (O15533)\n Function: Involved in the association of MHC class I with transporter associated with antigen processing (TAP) and in the assembly of MHC class I with peptide (peptide loading)\n Location: Endoplasmic reticulum membrane\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that C1QB and TAPBP co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 931028, "gene_symbol_1": "C1QB", "gene_symbol_2": "TAPBP", "detection_method": null}} +{"question_id": "PPIL1-0767", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: ATP6V0E1 (O15342)\n Function: Subunit of the V0 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates prot\n Location: Membrane\n\nProtein 2: PPARG (P37231)\n Function: Ligand-activated transcription factor that forms obligate heterodimers with the retinoic acid receptor and acts as a key regulator of biological processes, such as adipocyte differentiation, lipid met\n Location: Nucleus\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that ATP6V0E1 and PPARG co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1022049, "gene_symbol_1": "ATP6V0E1", "gene_symbol_2": "PPARG", "detection_method": null}} +{"question_id": "PPIL1-0768", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: RNASEH2B (Q5TBB1)\n Function: Non catalytic subunit of RNase H2, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki f\n Location: Nucleus\n\nProtein 2: SLBP (Q14493)\n Function: RNA-binding protein involved in the histone pre-mRNA processing (PubMed:12588979, PubMed:19155325, PubMed:8957003, PubMed:9049306). Binds the stem-loop structure of replication-dependent histone pre-m\n Location: Cytoplasm\n\nEvidence: Analysis of protein complex data indicates RNASEH2B and SLBP are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1198553, "gene_symbol_1": "RNASEH2B", "gene_symbol_2": "SLBP", "detection_method": null}} +{"question_id": "PPIL1-0769", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: SCNN1A (P37088)\n Function: This is one of the three pore-forming subunits of the heterotrimeric epithelial sodium channel (ENaC), a critical regulator of sodium balance and fluid homeostasis (PubMed:30251954, PubMed:32729833, P\n Location: Apical cell membrane\n\nProtein 2: ATP8A1 (Q9Y2Q0)\n Function: Catalytic component of a P4-ATPase flippase complex which catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids from the outer to the inner leaflet of various membranes and en\n Location: Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane\n\nEvidence: Analysis of protein complex data indicates SCNN1A and ATP8A1 are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 704754, "gene_symbol_1": "SCNN1A", "gene_symbol_2": "ATP8A1", "detection_method": null}} +{"question_id": "PPIL1-0770", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: WDR5 (P61964)\n Function: Contributes to histone modification (PubMed:16600877, PubMed:16829960, PubMed:19103755, PubMed:19131338, PubMed:19556245, PubMed:20018852). May position the N-terminus of histone H3 for efficient trim\n Location: Nucleus\n\nProtein 2: ERCC4 (Q92889)\n Function: Catalytic component of a structure-specific DNA repair endonuclease responsible for the 5-prime incision during DNA repair, and which is essential for nucleotide excision repair (NER) and interstrand \n Location: Nucleus\n\nEvidence: Analysis of protein complex data indicates WDR5 and ERCC4 are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 783893, "gene_symbol_1": "WDR5", "gene_symbol_2": "ERCC4", "detection_method": null}} +{"question_id": "PPIL1-0771", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: MAFK (O60675)\n Function: Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves (PubMed:9150357). However, they act as transcriptional activa\n Location: Nucleus\n\nProtein 2: SPC25 (Q9HBM1)\n Function: Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity (PubMed:14699129, PubMed:14738735). Required for\n Location: Nucleus\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that MAFK and SPC25 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1622309, "gene_symbol_1": "MAFK", "gene_symbol_2": "SPC25", "detection_method": null}} +{"question_id": "PPIL1-0772", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: TRAF3 (Q13114)\n Function: Cytoplasmic E3 ubiquitin ligase that regulates various signaling pathways, such as the NF-kappa-B, mitogen-activated protein kinase (MAPK) and interferon regulatory factor (IRF) pathways, and thus con\n Location: Cytoplasm\n\nProtein 2: NLRP3 (Q96P20)\n Function: Sensor component of the NLRP3 inflammasome, which mediates inflammasome activation in response to defects in membrane integrity, leading to secretion of inflammatory cytokines IL1B and IL18 and pyropt\n Location: Cytoplasm, cytosol\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that TRAF3 and NLRP3 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 938785, "gene_symbol_1": "TRAF3", "gene_symbol_2": "NLRP3", "detection_method": null}} +{"question_id": "PPIL1-0773", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: ATP6V1F (Q16864)\n Function: Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates prot\n Location: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane\n\nProtein 2: TSNAX (Q99598)\n Function: Acts in combination with TSN as an endonuclease involved in the activation of the RNA-induced silencing complex (RISC). Possible role in spermatogenesis\n Location: Cytoplasm, perinuclear region\n\nEvidence: Analysis of protein complex data indicates ATP6V1F and TSNAX are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 583239, "gene_symbol_1": "ATP6V1F", "gene_symbol_2": "TSNAX", "detection_method": null}} +{"question_id": "PPIL1-0774", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: EMC7 (Q9NPA0)\n Function: Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins (PubMed:29242\n Location: Endoplasmic reticulum membrane\n\nProtein 2: ITGAX (P20702)\n Function: Integrin alpha-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially important in mon\n Location: Cell membrane\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that EMC7 and ITGAX do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1481617, "gene_symbol_1": "EMC7", "gene_symbol_2": "ITGAX", "detection_method": null}} +{"question_id": "PPIL1-0775", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: KCNJ5 (P48544)\n Function: Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration o\n Location: Membrane\n\nProtein 2: CD8A (P01732)\n Function: Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primari\n Location: Cell membrane\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that KCNJ5 and CD8A co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1220103, "gene_symbol_1": "KCNJ5", "gene_symbol_2": "CD8A", "detection_method": null}} +{"question_id": "PPIL1-0776", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: SMARCA2 (P51531)\n Function: ATPase involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that c\n Location: Nucleus\n\nProtein 2: CACNA1S (Q13698)\n Function: Pore-forming, alpha-1S subunit of the voltage-gated calcium channel that gives rise to L-type calcium currents in skeletal muscle. Calcium channels containing the alpha-1S subunit play an important ro\n Location: Cell membrane, sarcolemma, T-tubule\n\nEvidence: Analysis of protein complex data indicates SMARCA2 and CACNA1S are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1680305, "gene_symbol_1": "SMARCA2", "gene_symbol_2": "CACNA1S", "detection_method": null}} +{"question_id": "PPIL1-0777", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: LAMTOR5 (O43504)\n Function: As part of the Ragulator complex it is involved in amino acid sensing and activation of mTORC1, a signaling complex promoting cell growth in response to growth factors, energy levels, and amino acids \n Location: Lysosome\n\nProtein 2: BCL2L2 (Q92843)\n Function: Promotes cell survival. Blocks dexamethasone-induced apoptosis. Mediates survival of postmitotic Sertoli cells by suppressing death-promoting activity of BAX\n Location: Mitochondrion membrane\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that LAMTOR5 and BCL2L2 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 930753, "gene_symbol_1": "LAMTOR5", "gene_symbol_2": "BCL2L2", "detection_method": null}} +{"question_id": "PPIL1-0778", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: CNOT4 (O95628)\n Function: Has E3 ubiquitin ligase activity, promoting ubiquitination and degradation of target proteins (PubMed:11823428, PubMed:22159038, PubMed:26575292). Involved in activation of the JAK/STAT pathway (PubMe\n Location: Cytoplasm\n\nProtein 2: TSC2 (P49815)\n Function: Catalytic component of the TSC-TBC complex, a multiprotein complex that acts as a negative regulator of the canonical mTORC1 complex, an evolutionarily conserved central nutrient sensor that stimulate\n Location: Lysosome membrane\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that CNOT4 and TSC2 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1188454, "gene_symbol_1": "CNOT4", "gene_symbol_2": "TSC2", "detection_method": null}} +{"question_id": "PPIL1-0779", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: SUPT5H (O00267)\n Function: Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II (PubMed:10075709, PubMed:10199401, PubMed:104\n Location: Nucleus\n\nProtein 2: SMC6 (Q96SB8)\n Function: Core component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the\n Location: Nucleus\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that SUPT5H and SMC6 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 549347, "gene_symbol_1": "SUPT5H", "gene_symbol_2": "SMC6", "detection_method": null}} +{"question_id": "PPIL1-0780", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: MAGOH (P61326)\n Function: Required for pre-mRNA splicing as component of the spliceosome (PubMed:11991638). Plays a redundant role with MAGOHB as core component of the exon junction complex (EJC) and in the nonsense-mediated d\n Location: Nucleus\n\nProtein 2: TNNI3 (P19429)\n Function: Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that MAGOH and TNNI3 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1150588, "gene_symbol_1": "MAGOH", "gene_symbol_2": "TNNI3", "detection_method": null}} +{"question_id": "PPIL1-0781", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: MSH6 (P52701)\n Function: Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha be\n Location: Nucleus\n\nProtein 2: TGFBR2 (P37173)\n Function: Transmembrane serine/threonine kinase forming with the TGF-beta type I serine/threonine kinase receptor, TGFBR1, the non-promiscuous receptor for the TGF-beta cytokines TGFB1, TGFB2 and TGFB3. Transdu\n Location: Cell membrane\n\nEvidence: Analysis of protein complex data indicates MSH6 and TGFBR2 are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 852967, "gene_symbol_1": "MSH6", "gene_symbol_2": "TGFBR2", "detection_method": null}} +{"question_id": "PPIL1-0782", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: IGLC3 (P0DOY3)\n Function: Constant region of immunoglobulin light chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral i\n Location: Secreted\n\nProtein 2: EXOSC9 (Q06265)\n Function: Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA \n Location: Cytoplasm\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that IGLC3 and EXOSC9 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1410042, "gene_symbol_1": "IGLC3", "gene_symbol_2": "EXOSC9", "detection_method": null}} +{"question_id": "PPIL1-0783", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: CCNH (P51946)\n Function: Regulates CDK7, the catalytic subunit of the CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK comp\n Location: Nucleus\n\nProtein 2: SF3A1 (Q15459)\n Function: Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs (PubMed:10882114, PubMed:11533230, PubMed:32494006). The 17S\n Location: Nucleus\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that CCNH and SF3A1 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1585842, "gene_symbol_1": "CCNH", "gene_symbol_2": "SF3A1", "detection_method": null}} +{"question_id": "PPIL1-0784", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: TRAPPC13 (A5PLN9)\n\nProtein 2: CLCN7 (P51798)\n Function: Slowly voltage-gated channel mediating the exchange of chloride ions against protons (PubMed:18449189, PubMed:21527911). Functions as antiporter and contributes to the acidification of the lysosome lu\n Location: Lysosome membrane\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that TRAPPC13 and CLCN7 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 559558, "gene_symbol_1": "TRAPPC13", "gene_symbol_2": "CLCN7", "detection_method": null}} +{"question_id": "PPIL1-0785", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: PRKCI (P41743)\n Function: Calcium- and diacylglycerol-independent serine/ threonine-protein kinase that plays a general protective role against apoptotic stimuli, is involved in NF-kappa-B activation, cell survival, differenti\n Location: Cytoplasm\n\nProtein 2: MORF4L1 (Q9UBU8)\n Function: Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modifi\n Location: Nucleus\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that PRKCI and MORF4L1 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 744093, "gene_symbol_1": "PRKCI", "gene_symbol_2": "MORF4L1", "detection_method": null}} +{"question_id": "PPIL1-0786", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: COQ3 (Q9NZJ6)\n Function: O-methyltransferase required for two non-consecutive steps during ubiquinone biosynthesis (By similarity) (PubMed:10777520, PubMed:38425362). Catalyzes the 2 O-methylation of 3,4-dihydroxy-5-(all-tran\n Location: Mitochondrion inner membrane\n\nProtein 2: IARS1 (P41252)\n Function: Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of th\n Location: Cytoplasm\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that COQ3 and IARS1 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1607448, "gene_symbol_1": "COQ3", "gene_symbol_2": "IARS1", "detection_method": null}} +{"question_id": "PPIL1-0787", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: SNRPE (P62304)\n Function: Plays a role in pre-mRNA splicing as a core component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome (PubMed:11991638, PubMed:1\n Location: Cytoplasm, cytosol\n\nProtein 2: WDR35 (Q9P2L0)\n Function: As a component of the IFT complex A (IFT-A), a complex required for retrograde ciliary transport and entry into cilia of G protein-coupled receptors (GPCRs), it is involved in ciliogenesis and ciliary\n Location: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that SNRPE and WDR35 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 773545, "gene_symbol_1": "SNRPE", "gene_symbol_2": "WDR35", "detection_method": null}} +{"question_id": "PPIL1-0788", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: NXF5 (Q9H1B4)\n Function: Could be involved in the export of mRNA from the nucleus to the cytoplasm. Could also have a role in polarized cytoplasmic transport and localization of mRNA in neurons\n Location: Cytoplasm\n\nProtein 2: EIF2S3 (P41091)\n Function: Member of the eIF2 complex that functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA (PubMed:31836389). This complex binds to a 40S ribosomal subu\n Location: Cytoplasm, cytosol\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that NXF5 and EIF2S3 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 987511, "gene_symbol_1": "NXF5", "gene_symbol_2": "EIF2S3", "detection_method": null}} +{"question_id": "PPIL1-0789", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: CDK5 (Q00535)\n Function: Proline-directed serine/threonine-protein kinase essential for neuronal cell cycle arrest and differentiation and may be involved in apoptotic cell death in neuronal diseases by triggering abortive ce\n Location: Cytoplasm\n\nProtein 2: AP5Z1 (O43299)\n Function: As part of AP-5, a probable fifth adaptor protein complex it may be involved in endosomal transport. According to PubMed:20613862 it is a putative helicase required for efficient homologous recombinat\n Location: Cytoplasm\n\nEvidence: Analysis of protein complex data indicates CDK5 and AP5Z1 are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 654469, "gene_symbol_1": "CDK5", "gene_symbol_2": "AP5Z1", "detection_method": null}} +{"question_id": "PPIL1-0790", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: CPSF6 (Q16630)\n Function: Component of the cleavage factor Im (CFIm) complex that functions as an activator of the pre-mRNA 3'-end cleavage and polyadenylation processing required for the maturation of pre-mRNA into functional\n Location: Nucleus\n\nProtein 2: STRAP (Q9Y3F4)\n Function: The SMN complex catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, and thereby plays an important role in the splicing of cellular pre-mRNAs. \n Location: Cytoplasm\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that CPSF6 and STRAP do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1651273, "gene_symbol_1": "CPSF6", "gene_symbol_2": "STRAP", "detection_method": null}} +{"question_id": "PPIL1-0791", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: GEMIN8 (Q9NWZ8)\n Function: The SMN complex catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, and thereby plays an important role in the splicing of cellular pre-mRNAs. \n Location: Nucleus, gem\n\nProtein 2: CHUK (O15111)\n Function: Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other \n Location: Cytoplasm\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that GEMIN8 and CHUK do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1360804, "gene_symbol_1": "GEMIN8", "gene_symbol_2": "CHUK", "detection_method": null}} +{"question_id": "PPIL1-0792", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: POLE4 (Q9NR33)\n Function: Accessory component of the DNA polymerase epsilon complex (PubMed:10801849). Participates in DNA repair and in chromosomal DNA replication (By similarity)\n Location: Nucleus\n\nProtein 2: MAML1 (Q92585)\n Function: Acts as a transcriptional coactivator for NOTCH proteins. Has been shown to amplify NOTCH-induced transcription of HES1. Enhances phosphorylation and proteolytic turnover of the NOTCH intracellular do\n Location: Nucleus speckle\n\nEvidence: Analysis of protein complex data indicates POLE4 and MAML1 are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1233513, "gene_symbol_1": "POLE4", "gene_symbol_2": "MAML1", "detection_method": null}} +{"question_id": "PPIL1-0793", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: CDC27 (P30260)\n Function: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle (PubMed:18485873)\n Location: Nucleus\n\nProtein 2: SPC25 (Q9HBM1)\n Function: Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity (PubMed:14699129, PubMed:14738735). Required for\n Location: Nucleus\n\nEvidence: Analysis of protein complex data indicates CDC27 and SPC25 are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1472737, "gene_symbol_1": "CDC27", "gene_symbol_2": "SPC25", "detection_method": null}} +{"question_id": "PPIL1-0794", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: FGB (P02675)\n Function: Cleaved by the protease thrombin to yield monomers which, together with fibrinogen alpha (FGA) and fibrinogen gamma (FGG), polymerize to form an insoluble fibrin matrix. Fibrin has a major function in\n Location: Secreted\n\nProtein 2: COL11A2 (P13942)\n Function: May play an important role in fibrillogenesis by controlling lateral growth of collagen II fibrils\n Location: Secreted, extracellular space, extracellular matrix\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that FGB and COL11A2 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1600271, "gene_symbol_1": "FGB", "gene_symbol_2": "COL11A2", "detection_method": null}} +{"question_id": "PPIL1-0795", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: PRKAA2 (P54646)\n Function: Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism (PubMed:17307971, PubMed:17712357). In response\n Location: Cytoplasm\n\nProtein 2: VPS33B (Q9H267)\n Function: May play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes. Required for proper trafficking and targeting of\n Location: Late endosome membrane\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that PRKAA2 and VPS33B co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1386583, "gene_symbol_1": "PRKAA2", "gene_symbol_2": "VPS33B", "detection_method": null}} +{"question_id": "PPIL1-0796", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: DCP1A (Q9NPI6)\n Function: Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay (PubMed:12417715). Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5\n Location: Cytoplasm, P-body\n\nProtein 2: CFB (P00751)\n Function: Precursor of the catalytic component of the C3 and C5 convertase complexes of the alternative pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathog\n Location: Secreted\n\nEvidence: Analysis of protein complex data indicates DCP1A and CFB are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1097582, "gene_symbol_1": "DCP1A", "gene_symbol_2": "CFB", "detection_method": null}} +{"question_id": "PPIL1-0797", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: BMAL1 (O00327)\n Function: Transcriptional activator which forms a core component of the circadian clock. The circadian clock, an internal time-keeping system, regulates various physiological processes through the generation of\n Location: Nucleus\n\nProtein 2: SF3A3 (Q12874)\n Function: Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs (PubMed:10882114, PubMed:11533230, PubMed:32494006, PubMed:3\n Location: Nucleus speckle\n\nEvidence: Analysis of protein complex data indicates BMAL1 and SF3A3 are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1282160, "gene_symbol_1": "BMAL1", "gene_symbol_2": "SF3A3", "detection_method": null}} +{"question_id": "PPIL1-0798", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: SNRNP200 (O75643)\n Function: Catalyzes the ATP-dependent unwinding of U4/U6 RNA duplices, an essential step in the assembly of a catalytically active spliceosome (PubMed:35241646). Plays a role in pre-mRNA splicing as a core comp\n Location: Nucleus\n\nProtein 2: IFT25 (Q9Y547)\n Function: Component of the IFT complex B required for sonic hedgehog/SHH signaling. May mediate transport of SHH components: required for the export of SMO and PTCH1 receptors out of the cilium and the accumula\n Location: Cell projection, cilium\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that SNRNP200 and IFT25 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1685828, "gene_symbol_1": "SNRNP200", "gene_symbol_2": "IFT25", "detection_method": null}} +{"question_id": "PPIL1-0799", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: MEN1 (O00255)\n Function: Essential component of a MLL/SET1 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3 (H3K4). Functions as a transcriptional regulator. Binds to the T\n Location: Nucleus\n\nProtein 2: PDHA1 (P08559)\n Function: Together with PDHB forms the heterotetrameric E1 subunit of the pyruvate dehydrogenase (PDH) complex (PubMed:17474719, PubMed:19081061). The PDH complex catalyzes the overall conversion of pyruvate to\n Location: Mitochondrion matrix\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that MEN1 and PDHA1 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 867683, "gene_symbol_1": "MEN1", "gene_symbol_2": "PDHA1", "detection_method": null}} +{"question_id": "PPIL1-0800", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: INO80E (Q8NBZ0)\n Function: Putative regulatory component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair\n Location: Nucleus\n\nProtein 2: HTR3E (A5X5Y0)\n Function: Forms serotonin (5-hydroxytryptamine/5-HT3)-activated cation-selective channel complexes, which when activated cause fast, depolarizing responses in neurons\n Location: Postsynaptic cell membrane\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that INO80E and HTR3E do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1118079, "gene_symbol_1": "INO80E", "gene_symbol_2": "HTR3E", "detection_method": null}} +{"question_id": "PPIL1-0801", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: FARSB (Q9NSD9)\n Location: Cytoplasm\n\nProtein 2: MOCS2 (O96033)\n Function: Acts as a sulfur carrier required for molybdopterin biosynthesis (PubMed:22453920). Component of the molybdopterin synthase complex that catalyzes the conversion of precursor Z into molybdopterin by m\n Location: Cytoplasm, cytosol\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that FARSB and MOCS2 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1154926, "gene_symbol_1": "FARSB", "gene_symbol_2": "MOCS2", "detection_method": null}} +{"question_id": "PPIL1-0802", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: HRAS (P01112)\n Function: Involved in the activation of Ras protein signal transduction (PubMed:22821884). Ras proteins bind GDP/GTP and possess intrinsic GTPase activity (PubMed:12740440, PubMed:14500341, PubMed:9020151)\n Location: Cell membrane\n\nProtein 2: HPS5 (Q9UPZ3)\n Function: May regulate the synthesis and function of lysosomes and of highly specialized organelles, such as melanosomes and platelet dense granules. Regulates intracellular vesicular trafficking in fibroblasts\n Location: Cytoplasm, cytosol\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that HRAS and HPS5 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 652466, "gene_symbol_1": "HRAS", "gene_symbol_2": "HPS5", "detection_method": null}} +{"question_id": "PPIL1-0803", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: IBA57 (Q5T440)\n Function: Mitochondrial protein involved in the maturation of mitochondrial [4Fe-4S]-proteins in the late stage of the iron-sulfur cluster assembly pathway (PubMed:22323289, PubMed:23462291). Operates in cooper\n Location: Mitochondrion\n\nProtein 2: COQ5 (Q5HYK3)\n Function: Methyltransferase required for the conversion of 2-decaprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-decaprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2)\n Location: Mitochondrion inner membrane\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that IBA57 and COQ5 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1539259, "gene_symbol_1": "IBA57", "gene_symbol_2": "COQ5", "detection_method": null}} +{"question_id": "PPIL1-0804", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: GAD1 (Q99259)\n Function: Catalyzes the synthesis of the inhibitory neurotransmitter gamma-aminobutyric acid (GABA) with pyridoxal 5'-phosphate as cofactor\n\nProtein 2: BBS5 (Q8N3I7)\n Function: The BBSome complex is thought to function as a coat complex required for sorting of specific membrane proteins to the primary cilia. The BBSome complex is required for ciliogenesis but is dispensable \n Location: Cell projection, cilium membrane\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that GAD1 and BBS5 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 884009, "gene_symbol_1": "GAD1", "gene_symbol_2": "BBS5", "detection_method": null}} +{"question_id": "PPIL1-0805", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: AP1S2 (P56377)\n Function: Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitmen\n Location: Golgi apparatus\n\nProtein 2: EPRS1 (P07814)\n Function: Multifunctional protein which primarily functions within the aminoacyl-tRNA synthetase multienzyme complex, also known as multisynthetase complex. Within the complex it catalyzes the attachment of bot\n Location: Cytoplasm, cytosol\n\nEvidence: Analysis of protein complex data indicates AP1S2 and EPRS1 are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1496473, "gene_symbol_1": "AP1S2", "gene_symbol_2": "EPRS1", "detection_method": null}} +{"question_id": "PPIL1-0806", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: F7 (P08709)\n Function: Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or t\n Location: Secreted\n\nProtein 2: CCNB1 (P14635)\n Function: Essential for the control of the cell cycle at the G2/M (mitosis) transition\n Location: Cytoplasm\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that F7 and CCNB1 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1085903, "gene_symbol_1": "F7", "gene_symbol_2": "CCNB1", "detection_method": null}} +{"question_id": "PPIL1-0807", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: FUZ (Q9BT04)\n Function: Probable planar cell polarity effector involved in cilium biogenesis. May regulate protein and membrane transport to the cilium. Proposed to function as core component of the CPLANE (ciliogenesis and \n Location: Cytoplasm\n\nProtein 2: TTI1 (O43156)\n Function: Regulator of the DNA damage response (DDR). Part of the TTT complex that is required to stabilize protein levels of the phosphatidylinositol 3-kinase-related protein kinase (PIKK) family proteins. The\n Location: Cytoplasm\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that FUZ and TTI1 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 707129, "gene_symbol_1": "FUZ", "gene_symbol_2": "TTI1", "detection_method": null}} +{"question_id": "PPIL1-0808", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: CHAF1B (Q13112)\n Function: Acts as a component of the histone chaperone complex chromatin assembly factor 1 (CAF-1), which assembles histone octamers onto DNA during replication and repair. CAF-1 performs the first step of the \n Location: Nucleus\n\nProtein 2: CEBPB (P17676)\n Function: Important transcription factor regulating the expression of genes involved in immune and inflammatory responses (PubMed:12048245, PubMed:1741402, PubMed:18647749, PubMed:9374525). Also plays a signifi\n Location: Nucleus\n\nEvidence: Analysis of protein complex data indicates CHAF1B and CEBPB are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 944145, "gene_symbol_1": "CHAF1B", "gene_symbol_2": "CEBPB", "detection_method": null}} +{"question_id": "PPIL1-0809", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: TAF9 (Q16594)\n Function: The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription (PubMed:33795473). TFIID recognizes and binds promoters with or \n Location: Nucleus\n\nProtein 2: LAMA4 (Q16363)\n Function: Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other ext\n Location: Secreted, extracellular space, extracellular matrix, basement membrane\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that TAF9 and LAMA4 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1514913, "gene_symbol_1": "TAF9", "gene_symbol_2": "LAMA4", "detection_method": null}} +{"question_id": "PPIL1-0810", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: YEATS4 (O95619)\n Function: Chromatin reader component of the NuA4 histone acetyltransferase (HAT) complex, a complex involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 a\n Location: Nucleus\n\nProtein 2: RBM26 (Q5T8P6)\n Function: May be involved in the turnover of nuclear polyadenylated (pA+) RNA\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that YEATS4 and RBM26 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 643152, "gene_symbol_1": "YEATS4", "gene_symbol_2": "RBM26", "detection_method": null}} +{"question_id": "PPIL1-0811", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: ATF7 (P17544)\n Function: Stress-responsive chromatin regulator that plays a role in various biological processes including innate immunological memory, adipocyte differentiation or telomerase regulation (PubMed:29490055). In \n Location: Nucleus\n\nProtein 2: SARNP (P82979)\n Function: Binds both single-stranded and double-stranded DNA with higher affinity for the single-stranded form. Specifically binds to scaffold/matrix attachment region DNA. Also binds single-stranded RNA. Enhan\n Location: Nucleus\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that ATF7 and SARNP do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 965287, "gene_symbol_1": "ATF7", "gene_symbol_2": "SARNP", "detection_method": null}} +{"question_id": "PPIL1-0812", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: HCFC1 (P51610)\n Function: Transcriptional coregulator (By similarity). Serves as a scaffold protein, bridging interactions between transcription factors, including THAP11 and ZNF143, and transcriptional coregulators (PubMed:26\n Location: Cytoplasm\n\nProtein 2: LSM4 (Q9Y4Z0)\n Function: Plays a role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex) (Pub\n Location: Nucleus\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that HCFC1 and LSM4 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1712799, "gene_symbol_1": "HCFC1", "gene_symbol_2": "LSM4", "detection_method": null}} +{"question_id": "PPIL1-0813", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: KMT2B (Q9UMN6)\n Function: Histone methyltransferase that catalyzes methyl group transfer from S-adenosyl-L-methionine to the epsilon-amino group of 'Lys-4' of histone H3 (H3K4) via a non-processive mechanism. Part of chromatin\n Location: Nucleus\n\nProtein 2: ITGA2B (P08514)\n Function: Integrin alpha-IIb/beta-3 (ITGA2B:ITGB3) is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. It recognizes the sequence R-G-D in a wide array of ligand\n Location: Cell membrane\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that KMT2B and ITGA2B do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1004722, "gene_symbol_1": "KMT2B", "gene_symbol_2": "ITGA2B", "detection_method": null}} +{"question_id": "PPIL1-0814", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: CYCS (P99999)\n Function: Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers \n Location: Mitochondrion intermembrane space\n\nProtein 2: ATP8B1 (O43520)\n Function: Catalytic component of a P4-ATPase flippase complex which catalyzes the hydrolysis of ATP coupled to the transport of phospholipids, in particular phosphatidylcholines (PC), from the outer to the inne\n Location: Cell membrane\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that CYCS and ATP8B1 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1552064, "gene_symbol_1": "CYCS", "gene_symbol_2": "ATP8B1", "detection_method": null}} +{"question_id": "PPIL1-0815", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: SNRPB2 (P08579)\n Function: Involved in pre-mRNA splicing as component of the spliceosome (PubMed:11991638, PubMed:28076346, PubMed:28502770, PubMed:28781166, PubMed:32494006). Associated with sn-RNP U2, where it contributes to \n Location: Nucleus\n\nProtein 2: CFB (P00751)\n Function: Precursor of the catalytic component of the C3 and C5 convertase complexes of the alternative pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathog\n Location: Secreted\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that SNRPB2 and CFB do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 774080, "gene_symbol_1": "SNRPB2", "gene_symbol_2": "CFB", "detection_method": null}} +{"question_id": "PPIL1-0816", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: SF3B5 (Q9BWJ5)\n Function: Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs (PubMed:12234937, PubMed:27720643, PubMed:28781166, PubMed:3\n Location: Nucleus\n\nProtein 2: SHARPIN (Q9H0F6)\n Function: Component of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation (PubMed:\n Location: Cytoplasm, cytosol\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that SF3B5 and SHARPIN co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 608159, "gene_symbol_1": "SF3B5", "gene_symbol_2": "SHARPIN", "detection_method": null}} +{"question_id": "PPIL1-0817", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: DLG2 (Q15700)\n Function: Required for perception of chronic pain through NMDA receptor signaling. Regulates surface expression of NMDA receptors in dorsal horn neurons of the spinal cord. Interacts with the cytoplasmic tail o\n Location: Cell membrane\n\nProtein 2: TLR10 (Q9BXR5)\n Function: Participates in the innate immune response to microbial agents. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (By similarity)\n Location: Membrane\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that DLG2 and TLR10 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 715844, "gene_symbol_1": "DLG2", "gene_symbol_2": "TLR10", "detection_method": null}} +{"question_id": "PPIL1-0818", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: WDR19 (Q8NEZ3)\n Function: As component of the IFT complex A (IFT-A), a complex required for retrograde ciliary transport and entry into cilia of G protein-coupled receptors (GPCRs), it is involved in cilia function and/or asse\n Location: Cell projection, cilium\n\nProtein 2: TLR4 (O00206)\n Function: Transmembrane receptor that functions as a pattern recognition receptor recognizing pathogen- and damage-associated molecular patterns (PAMPs and DAMPs) to induce innate immune responses via downstrea\n Location: Cell membrane\n\nEvidence: Analysis of protein complex data indicates WDR19 and TLR4 are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 990489, "gene_symbol_1": "WDR19", "gene_symbol_2": "TLR4", "detection_method": null}} +{"question_id": "PPIL1-0819", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: SMARCD1 (Q96GM5)\n Function: Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry ou\n Location: Nucleus\n\nProtein 2: RB1 (P06400)\n Function: Tumor suppressor that is a key regulator of the G1/S transition of the cell cycle (PubMed:10499802). The hypophosphorylated form binds transcription regulators of the E2F family, preventing transcript\n Location: Nucleus\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that SMARCD1 and RB1 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1016869, "gene_symbol_1": "SMARCD1", "gene_symbol_2": "RB1", "detection_method": null}} +{"question_id": "PPIL1-0820", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: STK11 (Q15831)\n Function: Tumor suppressor serine/threonine-protein kinase that controls the activity of AMP-activated protein kinase (AMPK) family members, thereby playing a role in various processes such as cell metabolism, \n Location: Nucleus\n\nProtein 2: DPM2 (O94777)\n Function: Regulates the biosynthesis of dolichol phosphate-mannose (PubMed:10835346). Regulatory subunit of the dolichol-phosphate mannose (DPM) synthase complex; essential for the ER localization and stable ex\n Location: Endoplasmic reticulum membrane\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that STK11 and DPM2 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1363610, "gene_symbol_1": "STK11", "gene_symbol_2": "DPM2", "detection_method": null}} +{"question_id": "PPIL1-0821", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: EXOSC3 (Q9NQT5)\n Function: Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA \n Location: Cytoplasm\n\nProtein 2: NCAPD3 (P42695)\n Function: Regulatory subunit of the condensin-2 complex, a complex which establishes mitotic chromosome architecture and is involved in physical rigidity of the chromatid axis (PubMed:14532007). May promote the\n Location: Nucleus\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that EXOSC3 and NCAPD3 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1263745, "gene_symbol_1": "EXOSC3", "gene_symbol_2": "NCAPD3", "detection_method": null}} +{"question_id": "PPIL1-0822", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: INO80D (Q53TQ3)\n Function: Putative regulatory component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair\n Location: Nucleus\n\nProtein 2: NSMCE4A (Q9NXX6)\n Function: Component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1\n Location: Nucleus\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that INO80D and NSMCE4A do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 592096, "gene_symbol_1": "INO80D", "gene_symbol_2": "NSMCE4A", "detection_method": null}} +{"question_id": "PPIL1-0823", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: CCT7 (Q99832)\n Function: Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of actin, tubulin and other proteins upon ATP hydrolysis (PubMed:25467444, PubMed:364937\n Location: Cytoplasm\n\nProtein 2: SUCLG2 (Q96I99)\n Function: GTP-specific succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of GTP and thus represents the only step of substrate-level phos\n Location: Mitochondrion\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that CCT7 and SUCLG2 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1286858, "gene_symbol_1": "CCT7", "gene_symbol_2": "SUCLG2", "detection_method": null}} +{"question_id": "PPIL1-0824", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: BCAS2 (O75934)\n Function: Required for pre-mRNA splicing as component of the activated spliceosome (PubMed:28076346, PubMed:28502770, PubMed:29301961, PubMed:29360106, PubMed:30705154). Component of the PRP19-CDC5L complex tha\n Location: Nucleus\n\nProtein 2: SPTSSA (Q969W0)\n Function: Component of the serine palmitoyltransferase multisubunit enzyme (SPT) that catalyzes the initial and rate-limiting step in sphingolipid biosynthesis by condensing L-serine and activated acyl-CoA (mos\n Location: Endoplasmic reticulum membrane\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that BCAS2 and SPTSSA co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 668595, "gene_symbol_1": "BCAS2", "gene_symbol_2": "SPTSSA", "detection_method": null}} +{"question_id": "PPIL1-0825", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: GTF2F2 (P13984)\n Function: TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB\n Location: Nucleus\n\nProtein 2: SNRPD2 (P62316)\n Function: Plays a role in pre-mRNA splicing as a core component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome (PubMed:11991638, PubMed:1\n Location: Cytoplasm, cytosol\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that GTF2F2 and SNRPD2 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 548622, "gene_symbol_1": "GTF2F2", "gene_symbol_2": "SNRPD2", "detection_method": null}} +{"question_id": "PPIL1-0826", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: TOMM6 (Q96B49)\n Function: Component of the translocase of the outer membrane of mitochondria (TOM) complex essential for the recognition and translocation of cytosolically synthesized mitochondrial preproteins (PubMed:40080546\n Location: Mitochondrion outer membrane\n\nProtein 2: CSTF1 (Q05048)\n Function: One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs (PubMed:10669729). May be responsible for the interaction of CSTF with other factors to form a stabl\n Location: Nucleus\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that TOMM6 and CSTF1 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1688966, "gene_symbol_1": "TOMM6", "gene_symbol_2": "CSTF1", "detection_method": null}} +{"question_id": "PPIL1-0827", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: SF3B4 (Q15427)\n Function: Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs (PubMed:10882114, PubMed:12234937, PubMed:27720643, PubMed:3\n Location: Nucleus\n\nProtein 2: UIMC1 (Q96RL1)\n Function: Ubiquitin-binding protein (PubMed:24627472). Specifically recognizes and binds 'Lys-63'-linked ubiquitin (PubMed:19328070, Ref.38). Plays a central role in the BRCA1-A complex by specifically binding \n Location: Nucleus\n\nEvidence: Analysis of protein complex data indicates SF3B4 and UIMC1 are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1060284, "gene_symbol_1": "SF3B4", "gene_symbol_2": "UIMC1", "detection_method": null}} +{"question_id": "PPIL1-0828", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: ERCC1 (P07992)\n Function: Non-catalytic component of a structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair. Responsible, in conjunction with SLX4, for the first step in the repair of in\n Location: Nucleus\n\nProtein 2: PPP3R2 (Q96LZ3)\n Function: Regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity\n Location: Mitochondrion\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that ERCC1 and PPP3R2 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 952539, "gene_symbol_1": "ERCC1", "gene_symbol_2": "PPP3R2", "detection_method": null}} +{"question_id": "PPIL1-0829", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: KAT2B (Q92831)\n Function: Functions as a histone acetyltransferase (HAT) to promote transcriptional activation (PubMed:8945521). Has significant histone acetyltransferase activity with core histones (H3 and H4), and also with \n Location: Nucleus\n\nProtein 2: NAA16 (Q6N069)\n Function: Auxillary subunit of the N-terminal acetyltransferase A (NatA) complex which displays alpha (N-terminal) acetyltransferase activity\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that KAT2B and NAA16 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 706513, "gene_symbol_1": "KAT2B", "gene_symbol_2": "NAA16", "detection_method": null}} +{"question_id": "PPIL1-0830", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: SMC3 (Q9UQE7)\n Function: Central component of cohesin, a complex required for chromosome cohesion during the cell cycle. The cohesin complex may form a large proteinaceous ring within which sister chromatids can be trapped. A\n Location: Nucleus\n\nProtein 2: RMI1 (Q9H9A7)\n Function: Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. Promotes TOP3A bin\n Location: Nucleus\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that SMC3 and RMI1 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1704320, "gene_symbol_1": "SMC3", "gene_symbol_2": "RMI1", "detection_method": null}} +{"question_id": "PPIL1-0831", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: CENPS (Q8N2Z9)\n Function: DNA-binding component of the Fanconi anemia (FA) core complex. Required for the normal activation of the FA pathway, leading to monoubiquitination of the FANCI-FANCD2 complex in response to DNA damage\n Location: Nucleus\n\nProtein 2: MIS12 (Q9H081)\n Function: Part of the MIS12 complex which is required for normal chromosome alignment and segregation and for kinetochore formation during mitosis (PubMed:12515822, PubMed:15502821, PubMed:16585270). Essential \n Location: Chromosome, centromere, kinetochore\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that CENPS and MIS12 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1139154, "gene_symbol_1": "CENPS", "gene_symbol_2": "MIS12", "detection_method": null}} +{"question_id": "PPIL1-0832", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: SMARCB1 (Q12824)\n Function: Core component of the BAF (hSWI/SNF) complex. This ATP-dependent chromatin-remodeling complex plays important roles in cell proliferation and differentiation, in cellular antiviral activities and inhi\n Location: Nucleus\n\nProtein 2: TEAD1 (P28347)\n Function: Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The cor\n Location: Nucleus\n\nEvidence: Analysis of protein complex data indicates SMARCB1 and TEAD1 are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1347156, "gene_symbol_1": "SMARCB1", "gene_symbol_2": "TEAD1", "detection_method": null}} +{"question_id": "PPIL1-0833", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: NELFA (Q9H3P2)\n Function: Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex\n Location: Nucleus\n\nProtein 2: WHRN (Q9P202)\n Function: Involved in hearing and vision as member of the USH2 complex. Necessary for elongation and maintenance of inner and outer hair cell stereocilia in the organ of Corti in the inner ear. Involved in the \n Location: Cytoplasm\n\nEvidence: Analysis of protein complex data indicates NELFA and WHRN are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1123386, "gene_symbol_1": "NELFA", "gene_symbol_2": "WHRN", "detection_method": null}} +{"question_id": "PPIL1-0834", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: NAA35 (Q5VZE5)\n Function: Auxillary component of the N-terminal acetyltransferase C (NatC) complex which catalyzes acetylation of N-terminal methionine residues (PubMed:19398576, PubMed:37891180). N-terminal acetylation protec\n Location: Cytoplasm\n\nProtein 2: MIS18A (Q9NYP9)\n Function: Required for recruitment of CENPA to centromeres and normal chromosome segregation during mitosis\n Location: Nucleus\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that NAA35 and MIS18A co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1421979, "gene_symbol_1": "NAA35", "gene_symbol_2": "MIS18A", "detection_method": null}} +{"question_id": "PPIL1-0835", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: ATP6V1G1 (O75348)\n Function: Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates prot\n Location: Apical cell membrane\n\nProtein 2: CTC1 (Q2NKJ3)\n Function: Component of the CST complex proposed to act as a specialized replication factor promoting DNA replication under conditions of replication stress or natural replication barriers such as the telomere d\n Location: Nucleus\n\nEvidence: Analysis of protein complex data indicates ATP6V1G1 and CTC1 are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 588007, "gene_symbol_1": "ATP6V1G1", "gene_symbol_2": "CTC1", "detection_method": null}} +{"question_id": "PPIL1-0836", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: GATC (O43716)\n Function: Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP throu\n Location: Mitochondrion\n\nProtein 2: RRM2 (P31350)\n Function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. Inhibits Wnt signaling\n Location: Cytoplasm\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that GATC and RRM2 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1157861, "gene_symbol_1": "GATC", "gene_symbol_2": "RRM2", "detection_method": null}} +{"question_id": "PPIL1-0837", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: QARS1 (P47897)\n Function: Glutamine--tRNA ligase (PubMed:26869582). Plays a critical role in brain development (PubMed:24656866)\n Location: Cytoplasm, cytosol\n\nProtein 2: MRPS9 (P82933)\n Location: Mitochondrion\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that QARS1 and MRPS9 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1565383, "gene_symbol_1": "QARS1", "gene_symbol_2": "MRPS9", "detection_method": null}} +{"question_id": "PPIL1-0838", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: CEBPE (Q15744)\n Function: Transcriptional activator (PubMed:26019275). C/EBP are DNA-binding proteins that recognize two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to ma\n Location: Nucleus\n\nProtein 2: EIF3I (Q13347)\n Function: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773, PubMed:274628\n Location: Cytoplasm\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that CEBPE and EIF3I do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1233811, "gene_symbol_1": "CEBPE", "gene_symbol_2": "EIF3I", "detection_method": null}} +{"question_id": "PPIL1-0839", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: SIN3A (Q96ST3)\n Function: Acts as a transcriptional repressor. Corepressor for REST. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Also interacts with MXD1-MAX heterodimers to rep\n Location: Nucleus\n\nProtein 2: SCRIB (Q14160)\n Function: Scaffold protein involved in different aspects of polarized cell differentiation regulating epithelial and neuronal morphogenesis and T-cell polarization (PubMed:15182672, PubMed:16344308, PubMed:1696\n Location: Cell membrane\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that SIN3A and SCRIB co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1030183, "gene_symbol_1": "SIN3A", "gene_symbol_2": "SCRIB", "detection_method": null}} +{"question_id": "PPIL1-0840", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: INTS5 (Q6P9B9)\n Function: Component of the integrator complex, a multiprotein complex that terminates RNA polymerase II (Pol II) transcription in the promoter-proximal region of genes (PubMed:33243860, PubMed:38570683). The in\n Location: Nucleus\n\nProtein 2: IL12B (P29460)\n Function: Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC\n Location: Secreted\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that INTS5 and IL12B do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1078962, "gene_symbol_1": "INTS5", "gene_symbol_2": "IL12B", "detection_method": null}} +{"question_id": "PPIL1-0841", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: F10 (P00742)\n Function: Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting (PubMed:22409427, PubMed:39880037). F\n Location: Secreted\n\nProtein 2: WDR5 (P61964)\n Function: Contributes to histone modification (PubMed:16600877, PubMed:16829960, PubMed:19103755, PubMed:19131338, PubMed:19556245, PubMed:20018852). May position the N-terminus of histone H3 for efficient trim\n Location: Nucleus\n\nEvidence: Analysis of protein complex data indicates F10 and WDR5 are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1123439, "gene_symbol_1": "F10", "gene_symbol_2": "WDR5", "detection_method": null}} +{"question_id": "PPIL1-0842", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: RBBP4 (Q09028)\n Function: Core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal \n Location: Nucleus\n\nProtein 2: KANSL2 (Q9H9L4)\n Function: Non-catalytic component of the NSL histone acetyltransferase complex, a multiprotein complex that mediates histone H4 acetylation at 'Lys-5'- and 'Lys-8' (H4K5ac and H4K8ac) at transcription start sit\n Location: Nucleus\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that RBBP4 and KANSL2 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1054498, "gene_symbol_1": "RBBP4", "gene_symbol_2": "KANSL2", "detection_method": null}} +{"question_id": "PPIL1-0843", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: TSEN54 (Q7Z6J9)\n Function: Non-catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5' and 3' splice site\n Location: Nucleus\n\nProtein 2: XPC (Q01831)\n Function: Involved in global genome nucleotide excision repair (GG-NER) by acting as damage sensing and DNA-binding factor component of the XPC complex (PubMed:10734143, PubMed:10873465, PubMed:12509299, PubMed\n Location: Nucleus\n\nEvidence: Analysis of protein complex data indicates TSEN54 and XPC are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 863077, "gene_symbol_1": "TSEN54", "gene_symbol_2": "XPC", "detection_method": null}} +{"question_id": "PPIL1-0844", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: SDCBP (O00560)\n Function: Multifunctional adapter protein involved in diverse array of functions including trafficking of transmembrane proteins, neuro and immunomodulation, exosome biogenesis, and tumorigenesis (PubMed:262915\n Location: Cell junction, focal adhesion\n\nProtein 2: CACNA1C (Q13936)\n Function: Pore-forming, alpha-1C subunit of the voltage-gated calcium channel that gives rise to L-type calcium currents (PubMed:12181424, PubMed:15454078, PubMed:15863612, PubMed:16299511, PubMed:17224476, Pub\n Location: Cell membrane\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that SDCBP and CACNA1C do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 685248, "gene_symbol_1": "SDCBP", "gene_symbol_2": "CACNA1C", "detection_method": null}} +{"question_id": "PPIL1-0845", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: ATG14 (Q6ZNE5)\n Function: Required for both basal and inducible autophagy. Determines the localization of the autophagy-specific PI3-kinase complex PI3KC3-C1 (PubMed:18843052, PubMed:19050071). Plays a role in autophagosome fo\n Location: Cytoplasm\n\nProtein 2: TAF5 (Q15542)\n Function: The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription (PubMed:33795473). TFIID recognizes and binds promoters with or \n Location: Nucleus\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that ATG14 and TAF5 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1155408, "gene_symbol_1": "ATG14", "gene_symbol_2": "TAF5", "detection_method": null}} +{"question_id": "PPIL1-0846", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: SMARCE1 (Q969G3)\n Function: Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry ou\n Location: Nucleus\n\nProtein 2: MDM2 (Q00987)\n Function: E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degradation by the proteasome (PubMed:29681526). Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apo\n Location: Nucleus, nucleoplasm\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that SMARCE1 and MDM2 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 674279, "gene_symbol_1": "SMARCE1", "gene_symbol_2": "MDM2", "detection_method": null}} +{"question_id": "PPIL1-0847", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: CRY2 (Q49AN0)\n Function: Transcriptional repressor which forms a core component of the circadian clock. The circadian clock, an internal time-keeping system, regulates various physiological processes through the generation of\n Location: Cytoplasm\n\nProtein 2: UIMC1 (Q96RL1)\n Function: Ubiquitin-binding protein (PubMed:24627472). Specifically recognizes and binds 'Lys-63'-linked ubiquitin (PubMed:19328070, Ref.38). Plays a central role in the BRCA1-A complex by specifically binding \n Location: Nucleus\n\nEvidence: Analysis of protein complex data indicates CRY2 and UIMC1 are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1028279, "gene_symbol_1": "CRY2", "gene_symbol_2": "UIMC1", "detection_method": null}} +{"question_id": "PPIL1-0848", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: MYBBP1A (Q9BQG0)\n Function: May activate or repress transcription via interactions with sequence specific DNA-binding proteins (By similarity). Repression may be mediated at least in part by histone deacetylase activity (HDAC ac\n Location: Cytoplasm\n\nProtein 2: CASTOR2 (A6NHX0)\n Function: Functions as a negative regulator of the TORC1 signaling pathway through the GATOR complex. As part of homodimers or heterodimers with CASTOR1, directly binds and inhibits the GATOR subcomplex GATOR2 \n Location: Cytoplasm, cytosol\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that MYBBP1A and CASTOR2 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1543389, "gene_symbol_1": "MYBBP1A", "gene_symbol_2": "CASTOR2", "detection_method": null}} +{"question_id": "PPIL1-0849", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: LAMTOR5 (O43504)\n Function: As part of the Ragulator complex it is involved in amino acid sensing and activation of mTORC1, a signaling complex promoting cell growth in response to growth factors, energy levels, and amino acids \n Location: Lysosome\n\nProtein 2: TELO2 (Q9Y4R8)\n Function: Regulator of the DNA damage response (DDR). Part of the TTT complex that is required to stabilize protein levels of the phosphatidylinositol 3-kinase-related protein kinase (PIKK) family proteins. The\n Location: Cytoplasm\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that LAMTOR5 and TELO2 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 820416, "gene_symbol_1": "LAMTOR5", "gene_symbol_2": "TELO2", "detection_method": null}} +{"question_id": "PPIL1-0850", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: AP4S1 (Q9Y587)\n Function: Component of the adaptor protein complex 4 (AP-4). Adaptor protein complexes are vesicle coat components involved both in vesicle formation and cargo selection. They control the vesicular transport of\n Location: Golgi apparatus, trans-Golgi network membrane\n\nProtein 2: COL9A1 (P20849)\n Function: Structural component of hyaline cartilage and vitreous of the eye\n Location: Secreted, extracellular space, extracellular matrix\n\nEvidence: Analysis of protein complex data indicates AP4S1 and COL9A1 are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1131789, "gene_symbol_1": "AP4S1", "gene_symbol_2": "COL9A1", "detection_method": null}} +{"question_id": "PPIL1-0851", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: CIAO1 (O76071)\n Function: Key component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into extramitochondrial Fe/S proteins (PubMed:1\n Location: Cytoplasm\n\nProtein 2: INTS11 (Q5TA45)\n Function: RNA endonuclease component of the integrator complex, a multiprotein complex that terminates RNA polymerase II (Pol II) transcription in the promoter-proximal region of genes (PubMed:16239144, PubMed:\n Location: Nucleus\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that CIAO1 and INTS11 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 535629, "gene_symbol_1": "CIAO1", "gene_symbol_2": "INTS11", "detection_method": null}} +{"question_id": "PPIL1-0852", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: GSK3B (P49841)\n Function: Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosph\n Location: Cytoplasm\n\nProtein 2: IL10RB (Q08334)\n Function: Shared cell surface receptor required for the activation of five class 2 cytokines: IL10, IL22, IL26, IL28, and IFNL1. The IFNLR1/IL10RB dimer is a receptor for the cytokine ligands IFNL2 and IFNL3 an\n Location: Membrane\n\nEvidence: Analysis of protein complex data indicates GSK3B and IL10RB are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1111960, "gene_symbol_1": "GSK3B", "gene_symbol_2": "IL10RB", "detection_method": null}} +{"question_id": "PPIL1-0853", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: KXD1 (Q9BQD3)\n Function: As part of the BORC complex may play a role in lysosomes movement and localization at the cell periphery. Associated with the cytosolic face of lysosomes, the BORC complex may recruit ARL8B and couple\n Location: Lysosome membrane\n\nProtein 2: SUCLG2 (Q96I99)\n Function: GTP-specific succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of GTP and thus represents the only step of substrate-level phos\n Location: Mitochondrion\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that KXD1 and SUCLG2 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1473912, "gene_symbol_1": "KXD1", "gene_symbol_2": "SUCLG2", "detection_method": null}} +{"question_id": "PPIL1-0854", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: ZWINT (O95229)\n Function: Acts as a component of the outer kinetochore KNL1 complex that serves as a docking point for spindle assembly checkpoint components and mediates microtubule-kinetochore interactions (PubMed:15094189, \n Location: Nucleus\n\nProtein 2: GTF2F2 (P13984)\n Function: TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB\n Location: Nucleus\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that ZWINT and GTF2F2 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1019678, "gene_symbol_1": "ZWINT", "gene_symbol_2": "GTF2F2", "detection_method": null}} +{"question_id": "PPIL1-0855", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: CRY2 (Q49AN0)\n Function: Transcriptional repressor which forms a core component of the circadian clock. The circadian clock, an internal time-keeping system, regulates various physiological processes through the generation of\n Location: Cytoplasm\n\nProtein 2: PSEN2 (P49810)\n Function: Catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid-beta precurso\n Location: Endoplasmic reticulum membrane\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that CRY2 and PSEN2 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1546862, "gene_symbol_1": "CRY2", "gene_symbol_2": "PSEN2", "detection_method": null}} +{"question_id": "PPIL1-0856", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: STRAP (Q9Y3F4)\n Function: The SMN complex catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, and thereby plays an important role in the splicing of cellular pre-mRNAs. \n Location: Cytoplasm\n\nProtein 2: IGHG4 (P01861)\n Function: Constant region of immunoglobulin (Ig) heavy chains. Igs are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound Igs se\n Location: Secreted\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that STRAP and IGHG4 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1256577, "gene_symbol_1": "STRAP", "gene_symbol_2": "IGHG4", "detection_method": null}} +{"question_id": "PPIL1-0857", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: ATF3 (P18847)\n Function: This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. I\n Location: Nucleus\n\nProtein 2: CCT8 (P50990)\n Function: Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of actin, tubulin and other proteins upon ATP hydrolysis (PubMed:25467444, PubMed:364937\n Location: Cytoplasm\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that ATF3 and CCT8 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1599998, "gene_symbol_1": "ATF3", "gene_symbol_2": "CCT8", "detection_method": null}} +{"question_id": "PPIL1-0858", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: CUL7 (Q14999)\n Function: Core component of the 3M and Cul7-RING(FBXW8) complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed:12481031, PubMed:12904573, PubMed:21572988, \n Location: Cytoplasm\n\nProtein 2: ISCA1 (Q9BUE6)\n Function: Involved in the maturation of mitochondrial 4Fe-4S proteins functioning late in the iron-sulfur cluster assembly pathway. Probably involved in the binding of an intermediate of Fe/S cluster assembly\n Location: Mitochondrion\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that CUL7 and ISCA1 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 938947, "gene_symbol_1": "CUL7", "gene_symbol_2": "ISCA1", "detection_method": null}} +{"question_id": "PPIL1-0859", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: RAD9A (Q99638)\n Function: Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair (PubMed:10713044, PubMed:17575048, PubMed:20545769, PubMed:21659603, PubMed:31135337). The 9-1-1 com\n Location: Nucleus\n\nProtein 2: SDHB (P21912)\n Function: Iron-sulfur protein (IP) subunit of the succinate dehydrogenase complex (mitochondrial respiratory chain complex II), responsible for transferring electrons from succinate to ubiquinone (coenzyme Q) (\n Location: Mitochondrion inner membrane\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that RAD9A and SDHB do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 600024, "gene_symbol_1": "RAD9A", "gene_symbol_2": "SDHB", "detection_method": null}} +{"question_id": "PPIL1-0860", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: ATG12 (O94817)\n Function: Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E\n Location: Cytoplasm\n\nProtein 2: H2BC11 (P06899)\n Function: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central r\n Location: Nucleus\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that ATG12 and H2BC11 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1203389, "gene_symbol_1": "ATG12", "gene_symbol_2": "H2BC11", "detection_method": null}} +{"question_id": "PPIL1-0861", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: SMARCC2 (Q8TAQ2)\n Function: Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry ou\n Location: Nucleus\n\nProtein 2: DDA1 (Q9BW61)\n Function: Functions as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins (Pu\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that SMARCC2 and DDA1 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1381332, "gene_symbol_1": "SMARCC2", "gene_symbol_2": "DDA1", "detection_method": null}} +{"question_id": "PPIL1-0862", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: FSHB (P01225)\n Function: Together with the alpha chain CGA constitutes follitropin, the follicle-stimulating hormone, and provides its biological specificity to the hormone heterodimer. Binds FSHR, a G protein-coupled recepto\n Location: Secreted\n\nProtein 2: STAG2 (Q8N3U4)\n Function: Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chroma\n Location: Nucleus\n\nEvidence: Analysis of protein complex data indicates FSHB and STAG2 are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 828399, "gene_symbol_1": "FSHB", "gene_symbol_2": "STAG2", "detection_method": null}} +{"question_id": "PPIL1-0863", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: COPS6 (Q7L5N1)\n Function: Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathwa\n Location: Nucleus\n\nProtein 2: PNPT1 (Q8TCS8)\n Function: RNA-binding protein implicated in numerous RNA metabolic processes (PubMed:29967381, PubMed:39019044). Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'-to-5' \n Location: Cytoplasm\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that COPS6 and PNPT1 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1076593, "gene_symbol_1": "COPS6", "gene_symbol_2": "PNPT1", "detection_method": null}} +{"question_id": "PPIL1-0864", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: UIMC1 (Q96RL1)\n Function: Ubiquitin-binding protein (PubMed:24627472). Specifically recognizes and binds 'Lys-63'-linked ubiquitin (PubMed:19328070, Ref.38). Plays a central role in the BRCA1-A complex by specifically binding \n Location: Nucleus\n\nProtein 2: NCF4 (Q15080)\n Function: Subunit of the phagocyte NADPH oxidase complex that mediates the transfer of electrons from cytosolic NADPH to O2 to produce the superoxide anion (O2(-)) (Probable). In the activated complex, electron\n Location: Cytoplasm, cytosol\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that UIMC1 and NCF4 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 507770, "gene_symbol_1": "UIMC1", "gene_symbol_2": "NCF4", "detection_method": null}} +{"question_id": "PPIL1-0865", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: CIAO1 (O76071)\n Function: Key component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into extramitochondrial Fe/S proteins (PubMed:1\n Location: Cytoplasm\n\nProtein 2: SPCS2 (Q15005)\n Function: Component of the signal peptidase complex (SPC) which catalyzes the cleavage of N-terminal signal sequences from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum (\n Location: Endoplasmic reticulum membrane\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that CIAO1 and SPCS2 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 684999, "gene_symbol_1": "CIAO1", "gene_symbol_2": "SPCS2", "detection_method": null}} +{"question_id": "PPIL1-0866", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: CDK8 (P49336)\n Function: Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gen\n Location: Nucleus\n\nProtein 2: GATAD2B (Q8WXI9)\n Function: Transcriptional repressor (PubMed:12183469, PubMed:16415179). Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin (PubMed:16428440, PubMed:289\n Location: Nucleus speckle\n\nEvidence: Analysis of protein complex data indicates CDK8 and GATAD2B are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 640765, "gene_symbol_1": "CDK8", "gene_symbol_2": "GATAD2B", "detection_method": null}} +{"question_id": "PPIL1-0867", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: C6 (P13671)\n Function: Component of the membrane attack complex (MAC), a multiprotein complex activated by the complement cascade, which inserts into a target cell membrane and forms a pore, leading to target cell membrane \n Location: Secreted\n\nProtein 2: PIK3R6 (Q5UE93)\n Function: Regulatory subunit of the PI3K gamma complex. Acts as an adapter to drive activation of PIK3CG by beta-gamma G protein dimers. The PIK3CG:PIK3R6 heterodimer is much less sensitive to beta-gamma G prot\n Location: Cytoplasm\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that C6 and PIK3R6 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1163349, "gene_symbol_1": "C6", "gene_symbol_2": "PIK3R6", "detection_method": null}} +{"question_id": "PPIL1-0868", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: OOEP (A6NGQ2)\n Function: Component of the subcortical maternal complex (SCMC), a multiprotein complex that plays a key role in early embryonic development. The SCMC complex is a structural constituent of cytoplasmic lattices,\n Location: Cytoplasm\n\nProtein 2: SDHB (P21912)\n Function: Iron-sulfur protein (IP) subunit of the succinate dehydrogenase complex (mitochondrial respiratory chain complex II), responsible for transferring electrons from succinate to ubiquinone (coenzyme Q) (\n Location: Mitochondrion inner membrane\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that OOEP and SDHB co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 871501, "gene_symbol_1": "OOEP", "gene_symbol_2": "SDHB", "detection_method": null}} +{"question_id": "PPIL1-0869", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: SNRPF (P62306)\n Function: Plays a role in pre-mRNA splicing as a core component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome (PubMed:11991638, PubMed:1\n Location: Cytoplasm, cytosol\n\nProtein 2: MRPS15 (P82914)\n Location: Mitochondrion matrix\n\nEvidence: Analysis of protein complex data indicates SNRPF and MRPS15 are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1155191, "gene_symbol_1": "SNRPF", "gene_symbol_2": "MRPS15", "detection_method": null}} +{"question_id": "PPIL1-0870", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: PSEN2 (P49810)\n Function: Catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid-beta precurso\n Location: Endoplasmic reticulum membrane\n\nProtein 2: GP1BB (P13224)\n Function: Gp-Ib, a surface membrane protein of platelets, participates in the formation of platelet plugs by binding to von Willebrand factor, which is already bound to the subendothelium\n Location: Membrane\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that PSEN2 and GP1BB co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1139265, "gene_symbol_1": "PSEN2", "gene_symbol_2": "GP1BB", "detection_method": null}} +{"question_id": "PPIL1-0871", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: KNL1 (Q8NG31)\n Function: Acts as a component of the outer kinetochore KNL1 complex that serves as a docking point for spindle assembly checkpoint components and mediates microtubule-kinetochore interactions (PubMed:15502821, \n Location: Nucleus\n\nProtein 2: SKIC3 (Q6PGP7)\n Function: Component of the SKI complex, a multiprotein complex that assists the RNA-degrading exosome during the mRNA decay and quality-control pathways (PubMed:16024656, PubMed:32006463, PubMed:35120588). The \n Location: Cytoplasm\n\nEvidence: Analysis of protein complex data indicates KNL1 and SKIC3 are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1532615, "gene_symbol_1": "KNL1", "gene_symbol_2": "SKIC3", "detection_method": null}} +{"question_id": "PPIL1-0872", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: NUS1 (Q96E22)\n Function: With DHDDS, forms the dehydrodolichyl diphosphate synthase (DDS) complex, an essential component of the dolichol monophosphate (Dol-P) biosynthetic machinery (PubMed:21572394, PubMed:25066056, PubMed:\n Location: Endoplasmic reticulum membrane\n\nProtein 2: DROSHA (Q9NRR4)\n Function: Ribonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to pro\n Location: Nucleus\n\nEvidence: Analysis of protein complex data indicates NUS1 and DROSHA are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1236601, "gene_symbol_1": "NUS1", "gene_symbol_2": "DROSHA", "detection_method": null}} +{"question_id": "PPIL1-0873", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: EIF4A3 (P38919)\n Function: ATP-dependent RNA helicase (PubMed:16170325). Involved in pre-mRNA splicing as component of the spliceosome (PubMed:11991638, PubMed:22961380, PubMed:28076346, PubMed:28502770, PubMed:29301961). Core \n Location: Nucleus\n\nProtein 2: MED7 (O43513)\n Function: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene\n Location: Nucleus\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that EIF4A3 and MED7 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 592128, "gene_symbol_1": "EIF4A3", "gene_symbol_2": "MED7", "detection_method": null}} +{"question_id": "PPIL1-0874", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: DDOST (P39656)\n Function: Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to a\n Location: Endoplasmic reticulum membrane\n\nProtein 2: VPS11 (Q9H270)\n Function: Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act as a core component of the putative H\n Location: Endosome\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that DDOST and VPS11 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1046967, "gene_symbol_1": "DDOST", "gene_symbol_2": "VPS11", "detection_method": null}} +{"question_id": "PPIL1-0875", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: CACNB1 (Q02641)\n Function: Regulatory subunit of L-type calcium channels (PubMed:1309651, PubMed:15615847, PubMed:8107964). Regulates the activity of L-type calcium channels that contain CACNA1A as pore-forming subunit (By simi\n Location: Cell membrane, sarcolemma\n\nProtein 2: BORCS5 (Q969J3)\n Function: As part of the BORC complex may play a role in lysosomes movement and localization at the cell periphery. Associated with the cytosolic face of lysosomes, the BORC complex may recruit ARL8B and couple\n Location: Lysosome membrane\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that CACNB1 and BORCS5 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1590753, "gene_symbol_1": "CACNB1", "gene_symbol_2": "BORCS5", "detection_method": null}} +{"question_id": "PPIL1-0876", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: ELP3 (Q9H9T3)\n Function: Catalytic tRNA acetyltransferase subunit of the elongator complex which is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl\n Location: Cytoplasm\n\nProtein 2: EMC10 (Q5UCC4)\n Function: Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins (PubMed:29242\n Location: Endoplasmic reticulum membrane\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that ELP3 and EMC10 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1528503, "gene_symbol_1": "ELP3", "gene_symbol_2": "EMC10", "detection_method": null}} +{"question_id": "PPIL1-0877", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: NBAS (A2RRP1)\n Function: Involved in Golgi-to-endoplasmic reticulum (ER) retrograde transport; the function is proposed to depend on its association in the NRZ complex which is believed to play a role in SNARE assembly at the\n Location: Cytoplasm\n\nProtein 2: SGCE (O43556)\n Function: Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix\n Location: Cell membrane, sarcolemma\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that NBAS and SGCE co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1592593, "gene_symbol_1": "NBAS", "gene_symbol_2": "SGCE", "detection_method": null}} +{"question_id": "PPIL1-0878", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: DEK (P35659)\n Function: Involved in chromatin organization\n Location: Nucleus\n\nProtein 2: ANAPC5 (Q9UJX4)\n Function: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle (PubMed:18485873)\n Location: Nucleus\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that DEK and ANAPC5 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1389807, "gene_symbol_1": "DEK", "gene_symbol_2": "ANAPC5", "detection_method": null}} +{"question_id": "PPIL1-0879", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: SMARCD1 (Q96GM5)\n Function: Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry ou\n Location: Nucleus\n\nProtein 2: ING1 (Q9UK53)\n Function: Cooperates with p53/TP53 in the negative regulatory pathway of cell growth by modulating p53-dependent transcriptional activation. Implicated as a tumor suppressor gene\n Location: Nucleus\n\nEvidence: Analysis of protein complex data indicates SMARCD1 and ING1 are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 957422, "gene_symbol_1": "SMARCD1", "gene_symbol_2": "ING1", "detection_method": null}} +{"question_id": "PPIL1-0880", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: CCNK (O75909)\n Function: Regulatory subunit of cyclin-dependent kinases that mediates activation of target kinases. Plays a role in transcriptional regulation via its role in regulating the phosphorylation of the C-terminal d\n Location: Nucleus\n\nProtein 2: MED12 (Q93074)\n Function: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene\n Location: Nucleus\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that CCNK and MED12 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 592905, "gene_symbol_1": "CCNK", "gene_symbol_2": "MED12", "detection_method": null}} +{"question_id": "PPIL1-0881", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: TOMM22 (Q9NS69)\n Function: Central receptor component of the translocase of the outer membrane of mitochondria (TOM) complex essential for the recognition and translocation of cytosolically synthesized mitochondrial preproteins\n Location: Mitochondrion outer membrane\n\nProtein 2: ATR (Q13535)\n Function: Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a D\n Location: Nucleus\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that TOMM22 and ATR do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1297299, "gene_symbol_1": "TOMM22", "gene_symbol_2": "ATR", "detection_method": null}} +{"question_id": "PPIL1-0882", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: RBBP4 (Q09028)\n Function: Core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal \n Location: Nucleus\n\nProtein 2: MSH6 (P52701)\n Function: Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha be\n Location: Nucleus\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that RBBP4 and MSH6 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 575549, "gene_symbol_1": "RBBP4", "gene_symbol_2": "MSH6", "detection_method": null}} +{"question_id": "PPIL1-0883", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: SMARCB1 (Q12824)\n Function: Core component of the BAF (hSWI/SNF) complex. This ATP-dependent chromatin-remodeling complex plays important roles in cell proliferation and differentiation, in cellular antiviral activities and inhi\n Location: Nucleus\n\nProtein 2: GET3 (O43681)\n Function: ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum (PubMed:17382883). Recognizes and selectively binds the transmembrane domain of TA prote\n Location: Cytoplasm\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that SMARCB1 and GET3 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 563674, "gene_symbol_1": "SMARCB1", "gene_symbol_2": "GET3", "detection_method": null}} +{"question_id": "PPIL1-0884", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: MMGT1 (Q8N4V1)\n Function: Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins (PubMed:29242\n Location: Endoplasmic reticulum membrane\n\nProtein 2: LLGL2 (Q6P1M3)\n Function: Part of a complex with GPSM2/LGN, PRKCI/aPKC and PARD6B/Par-6, which may ensure the correct organization and orientation of bipolar spindles for normal cell division. This complex plays roles in the i\n Location: Cytoplasm\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that MMGT1 and LLGL2 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1346840, "gene_symbol_1": "MMGT1", "gene_symbol_2": "LLGL2", "detection_method": null}} +{"question_id": "PPIL1-0885", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: CD8A (P01732)\n Function: Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primari\n Location: Cell membrane\n\nProtein 2: STAG3 (Q9UJ98)\n Function: Meiosis specific component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous r\n Location: Nucleus\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that CD8A and STAG3 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1448344, "gene_symbol_1": "CD8A", "gene_symbol_2": "STAG3", "detection_method": null}} +{"question_id": "PPIL1-0886", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: H4C1 (P62805)\n Function: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central r\n Location: Nucleus\n\nProtein 2: MUS81 (Q96NY9)\n Function: Catalytic subunit of two functionally distinct, structure-specific, heterodimeric DNA endonucleases MUS81-EME1 and MUS81-EME2 that are involved in the maintenance of genome stability (PubMed:11741546,\n Location: Nucleus, nucleolus\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that H4C1 and MUS81 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1215666, "gene_symbol_1": "H4C1", "gene_symbol_2": "MUS81", "detection_method": null}} +{"question_id": "PPIL1-0887", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: PYCARD (Q9ULZ3)\n Function: Functions as a key mediator in apoptosis and inflammation (PubMed:11103777, PubMed:12646168, PubMed:15030775, PubMed:17349957, PubMed:17599095, PubMed:19158675, PubMed:19158676, PubMed:19234215, PubMe\n Location: Cytoplasm\n\nProtein 2: MTA3 (Q9BTC8)\n Function: Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin (PubMed:12705869, PubMed:16428440, PubMed:28977666). Plays a role in maintenance of the no\n Location: Nucleus\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that PYCARD and MTA3 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 789861, "gene_symbol_1": "PYCARD", "gene_symbol_2": "MTA3", "detection_method": null}} +{"question_id": "PPIL1-0888", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: CENPN (Q96H22)\n Function: Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC compl\n Location: Nucleus\n\nProtein 2: TAF6L (Q9Y6J9)\n Function: Functions as a component of the PCAF complex. The PCAF complex is capable of efficiently acetylating histones in a nucleosomal context. The PCAF complex could be considered as the human version of the\n Location: Nucleus\n\nEvidence: Analysis of protein complex data indicates CENPN and TAF6L are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1505124, "gene_symbol_1": "CENPN", "gene_symbol_2": "TAF6L", "detection_method": null}} +{"question_id": "PPIL1-0889", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: GUCY1A2 (P33402)\n Function: Has guanylyl cyclase on binding to the beta-1 subunit\n Location: Cytoplasm\n\nProtein 2: WDR82 (Q6UXN9)\n Function: Regulatory component of the SET1/COMPASS complex implicated in the tethering of this complex to transcriptional start sites of active genes (PubMed:17998332, PubMed:18838538, PubMed:20516061). Facilit\n Location: Nucleus\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that GUCY1A2 and WDR82 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1356961, "gene_symbol_1": "GUCY1A2", "gene_symbol_2": "WDR82", "detection_method": null}} +{"question_id": "PPIL1-0890", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: SGCB (Q16585)\n Function: Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix\n Location: Cell membrane, sarcolemma\n\nProtein 2: DPY30 (Q9C005)\n Function: As part of the MLL1/MLL complex, involved in the methylation of histone H3 at 'Lys-4', particularly trimethylation. Histone H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptio\n Location: Nucleus\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that SGCB and DPY30 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1587566, "gene_symbol_1": "SGCB", "gene_symbol_2": "DPY30", "detection_method": null}} +{"question_id": "PPIL1-0891", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: NFIL3 (Q16649)\n Function: Acts as a transcriptional regulator that recognizes and binds to the sequence 5'-[GA]TTA[CT]GTAA[CT]-3', a sequence present in many cellular and viral promoters. Represses transcription from promoters\n Location: Nucleus\n\nProtein 2: ERGIC3 (Q9Y282)\n Function: Possible role in transport between endoplasmic reticulum and Golgi. Positively regulates trafficking of the secretory proteins SERPINA1/alpha1-antitrypsin and HP/haptoglobin (PubMed:31142615)\n Location: Endoplasmic reticulum-Golgi intermediate compartment membrane\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that NFIL3 and ERGIC3 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 767590, "gene_symbol_1": "NFIL3", "gene_symbol_2": "ERGIC3", "detection_method": null}} +{"question_id": "PPIL1-0892", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: SNRPB2 (P08579)\n Function: Involved in pre-mRNA splicing as component of the spliceosome (PubMed:11991638, PubMed:28076346, PubMed:28502770, PubMed:28781166, PubMed:32494006). Associated with sn-RNP U2, where it contributes to \n Location: Nucleus\n\nProtein 2: CIAO2A (Q9H5X1)\n Function: Component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into extramitochondrial Fe/S proteins (PubMed:23891\n Location: Cytoplasm\n\nEvidence: Analysis of protein complex data indicates SNRPB2 and CIAO2A are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 813448, "gene_symbol_1": "SNRPB2", "gene_symbol_2": "CIAO2A", "detection_method": null}} +{"question_id": "PPIL1-0893", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: UQCRB (P14927)\n Function: Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation. The resp\n Location: Mitochondrion inner membrane\n\nProtein 2: NCAPH2 (Q6IBW4)\n Function: Regulatory subunit of the condensin-2 complex, a complex that seems to provide chromosomes with an additional level of organization and rigidity and in establishing mitotic chromosome architecture (Pu\n Location: Nucleus\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that UQCRB and NCAPH2 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1347501, "gene_symbol_1": "UQCRB", "gene_symbol_2": "NCAPH2", "detection_method": null}} +{"question_id": "PPIL1-0894", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: ATXN7L3 (Q14CW9)\n Function: Component of the transcription regulatory histone acetylation (HAT) complex SAGA, a multiprotein complex that activates transcription by remodeling chromatin and mediating histone acetylation and deub\n Location: Nucleus\n\nProtein 2: COLEC10 (Q9Y6Z7)\n Function: Lectin that binds to various sugars: galactose > mannose = fucose > N-acetylglucosamine > N-acetylgalactosamine (PubMed:10224141). Acts as a chemoattractant, probably involved in the regulation of cel\n Location: Secreted\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that ATXN7L3 and COLEC10 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1478771, "gene_symbol_1": "ATXN7L3", "gene_symbol_2": "COLEC10", "detection_method": null}} +{"question_id": "PPIL1-0895", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: NPRL3 (Q12980)\n Function: As a component of the GATOR1 complex functions as an inhibitor of the amino acid-sensing branch of the mTORC1 pathway (PubMed:23723238, PubMed:29590090, PubMed:35338845). In response to amino acid dep\n Location: Lysosome membrane\n\nProtein 2: MED17 (Q9NVC6)\n Function: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene\n Location: Nucleus\n\nEvidence: Analysis of protein complex data indicates NPRL3 and MED17 are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 627802, "gene_symbol_1": "NPRL3", "gene_symbol_2": "MED17", "detection_method": null}} +{"question_id": "PPIL1-0896", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: INTU (Q9ULD6)\n Function: Plays a key role in ciliogenesis and embryonic development. Regulator of cilia formation by controlling the organization of the apical actin cytoskeleton and the positioning of the basal bodies at the\n Location: Cytoplasm\n\nProtein 2: INTS1 (Q8N201)\n Function: Component of the integrator complex, a multiprotein complex that terminates RNA polymerase II (Pol II) transcription in the promoter-proximal region of genes (PubMed:25201415, PubMed:33243860, PubMed:\n Location: Nucleus\n\nEvidence: Analysis of protein complex data indicates INTU and INTS1 are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 883086, "gene_symbol_1": "INTU", "gene_symbol_2": "INTS1", "detection_method": null}} +{"question_id": "PPIL1-0897", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: C6 (P13671)\n Function: Component of the membrane attack complex (MAC), a multiprotein complex activated by the complement cascade, which inserts into a target cell membrane and forms a pore, leading to target cell membrane \n Location: Secreted\n\nProtein 2: SF3B1 (O75533)\n Function: Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs (PubMed:12234937, PubMed:27720643, PubMed:32494006, PubMed:3\n Location: Nucleus\n\nEvidence: Computational analysis of proteomics data across multiple experiments found no evidence that C6 and SF3B1 co-fractionate or belong to the same complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 590633, "gene_symbol_1": "C6", "gene_symbol_2": "SF3B1", "detection_method": null}} +{"question_id": "PPIL1-0898", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: COX7C (P15954)\n Function: Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes suc\n Location: Mitochondrion inner membrane\n\nProtein 2: TCP1 (P17987)\n Function: Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of actin, tubulin and other proteins upon ATP hydrolysis (PubMed:25467444, PubMed:364937\n Location: Cytoplasm, cytosol\n\nEvidence: Machine learning analysis of co-fractionation mass spectrometry data from multiple cell types predicted that COX7C and TCP1 do not co-exist in the same protein complex.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 1204951, "gene_symbol_1": "COX7C", "gene_symbol_2": "TCP1", "detection_method": null}} +{"question_id": "PPIL1-0899", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: DDX6 (P26196)\n Function: Essential for the formation of P-bodies, cytosolic membrane-less ribonucleoprotein granules involved in RNA metabolism through the coordinated storage of mRNAs encoding regulatory functions (PubMed:25\n Location: Cytoplasm, P-body\n\nProtein 2: GRIN2B (Q13224)\n Function: Component of N-methyl-D-aspartate (NMDA) receptors (NMDARs) that function as heterotetrameric, ligand-gated cation channels with high calcium permeability and voltage-dependent block by Mg(2+) (PubMed\n Location: Cell membrane\n\nEvidence: Analysis of protein complex data indicates DDX6 and GRIN2B are unlikely to be found in the same molecular assembly.", "gold_answer": "C", "gold_category": "computational_inference", "metadata": {"source_db": "humap", "confidence_tier": "silver", "result_id": 915277, "gene_symbol_1": "DDX6", "gene_symbol_2": "GRIN2B", "detection_method": null}} +{"question_id": "PPIL1-0900", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: MUC17 (Q685J3)\n Function: Probably plays a role in maintaining homeostasis on mucosal surfaces\n Location: Cell membrane\n\nProtein 2: TRIM33 (Q9UPN9)\n Function: Acts as an E3 ubiquitin-protein ligase. Promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway. According to PubMed:16751102, does not promote a decrease\n Location: Nucleus\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between MUC17 and TRIM33 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2105194, "gene_symbol_1": "MUC17", "gene_symbol_2": "TRIM33", "detection_method": null}} +{"question_id": "PPIL1-0901", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: PSMD11 (O00231)\n Function: Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by \n Location: Nucleus\n\nProtein 2: DEFB107A (Q8IZN7)\n Function: Has antibacterial activity\n Location: Secreted\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between PSMD11 and DEFB107A is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1784516, "gene_symbol_1": "PSMD11", "gene_symbol_2": "DEFB107A", "detection_method": null}} +{"question_id": "PPIL1-0902", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: DOP1A (Q5JWR5)\n Function: May be involved in protein traffic between late Golgi and early endosomes\n Location: Golgi apparatus membrane\n\nProtein 2: PCDH12 (Q9NPG4)\n Function: Cellular adhesion molecule that may play an important role in cell-cell interactions at interendothelial junctions (By similarity). Acts as a regulator of cell migration, probably via increasing cell-\n Location: Cell membrane\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between DOP1A and PCDH12.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2089708, "gene_symbol_1": "DOP1A", "gene_symbol_2": "PCDH12", "detection_method": null}} +{"question_id": "PPIL1-0903", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: DLG1 (Q12959)\n Function: Essential multidomain scaffolding protein required for normal development (By similarity). Recruits channels, receptors and signaling molecules to discrete plasma membrane domains in polarized cells. \n Location: Cell membrane\n\nProtein 2: SLC44A1 (Q8WWI5)\n Function: Choline/H+ antiporter (PubMed:19357133, PubMed:23651124, PubMed:31855247, PubMed:33789160). Also acts as a high-affinity ethanolamine/H+ antiporter, regulating the supply of extracellular ethanolamine\n Location: Cell membrane\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between DLG1 and SLC44A1 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2037243, "gene_symbol_1": "DLG1", "gene_symbol_2": "SLC44A1", "detection_method": null}} +{"question_id": "PPIL1-0904", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: RCN2 (Q14257)\n Function: Not known. Binds calcium\n Location: Endoplasmic reticulum lumen\n\nProtein 2: COXFA4L2 (Q9NRX3)\n Function: Mitochondrial protein that plays a regulatory role in cellular metabolism, particularly under hypoxic conditions (PubMed:22100406). Downregulates mitochondrial respiratory chain complex I activity und\n Location: Mitochondrion inner membrane\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between RCN2 and COXFA4L2 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2050224, "gene_symbol_1": "RCN2", "gene_symbol_2": "COXFA4L2", "detection_method": null}} +{"question_id": "PPIL1-0905", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: MYZAP (P0CAP1)\n Function: Plays a role in cellular signaling via Rho-related GTP-binding proteins and subsequent activation of transcription factor SRF (By similarity). Targets TJP1 to cell junctions. In cortical neurons, may \n Location: Cytoplasm, cytoskeleton\n\nProtein 2: HCFC1R1 (Q9NWW0)\n Function: Regulates HCFC1 activity by modulating its subcellular localization. Overexpression of HCFC1R1 leads to accumulation of HCFC1 in the cytoplasm. HCFC1R1-mediated export may provide the pool of cytoplas\n Location: Cytoplasm\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between MYZAP and HCFC1R1 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1893682, "gene_symbol_1": "MYZAP", "gene_symbol_2": "HCFC1R1", "detection_method": null}} +{"question_id": "PPIL1-0906", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: MSGN1 (A6NI15)\n Function: Involved in specifying the paraxial, but not dorsal, mesoderm. May regulate the expression of T-box transcription factors required for mesoderm formation and differentiation (By similarity)\n Location: Nucleus\n\nProtein 2: OVGP1 (Q12889)\n Function: Binds to oocyte zona pellucida in vivo. May play a role in the fertilization process and/or early embryonic development\n Location: Cytoplasmic vesicle, secretory vesicle\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between MSGN1 and OVGP1.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1750793, "gene_symbol_1": "MSGN1", "gene_symbol_2": "OVGP1", "detection_method": null}} +{"question_id": "PPIL1-0907", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: MKRN1 (Q9UHC7)\n Function: E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. These substrates include FILIP1, p53/TP53, CDKN1A and TERT. Keeps cells alive by suppressing p53/T\n\nProtein 2: GLA (P06280)\n Function: Catalyzes the hydrolysis of glycosphingolipids and participates in their degradation in the lysosome\n Location: Lysosome\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between MKRN1 and GLA.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1880744, "gene_symbol_1": "MKRN1", "gene_symbol_2": "GLA", "detection_method": null}} +{"question_id": "PPIL1-0908", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: OR10A4 (Q9H209)\n Function: Odorant receptor (Potential). May be involved in taste perception\n Location: Cell membrane\n\nProtein 2: GPLD1 (P80108)\n Function: Hydrolyzes the inositol phosphate linkage of glycosylphosphatidylinositol (GPI)-anchored membrane proteins, thereby releasing them from the cell surface. Cleaves on the inositol side of the anchor, le\n Location: Secreted\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between OR10A4 and GPLD1.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2015779, "gene_symbol_1": "OR10A4", "gene_symbol_2": "GPLD1", "detection_method": null}} +{"question_id": "PPIL1-0909", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: C3orf49 (Q96BT1)\n\nProtein 2: ANKRD22 (Q5VYY1)\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between C3orf49 and ANKRD22 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2102751, "gene_symbol_1": "C3orf49", "gene_symbol_2": "ANKRD22", "detection_method": null}} +{"question_id": "PPIL1-0910", "task": "ppi-l1", "split": "fewshot", "difficulty": "hard", "context_text": "Protein 1: PMM1 (Q92871)\n Function: Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions. In addition, may be responsible for the degradation of glucos\n Location: Cytoplasm\n\nProtein 2: DNAJA3 (Q96EY1)\n Function: Modulates apoptotic signal transduction or effector structures within the mitochondrial matrix. Affect cytochrome C release from the mitochondria and caspase 3 activation, but not caspase 8 activation\n Location: Mitochondrion matrix\n\nEvidence: No significant association between PMM1 and DNAJA3 was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2195547, "gene_symbol_1": "PMM1", "gene_symbol_2": "DNAJA3", "detection_method": null}} +{"question_id": "PPIL1-0911", "task": "ppi-l1", "split": "fewshot", "difficulty": "hard", "context_text": "Protein 1: PSD3 (Q9NYI0)\n Function: Guanine nucleotide exchange factor for ARF6\n Location: Cell membrane\n\nProtein 2: GLP2R (O95838)\n Function: This is a receptor for glucagon-like peptide 2. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase\n Location: Cell membrane\n\nEvidence: No significant association between PSD3 and GLP2R was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1862844, "gene_symbol_1": "PSD3", "gene_symbol_2": "GLP2R", "detection_method": null}} +{"question_id": "PPIL1-0912", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: TRIM33 (Q9UPN9)\n Function: Acts as an E3 ubiquitin-protein ligase. Promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway. According to PubMed:16751102, does not promote a decrease\n Location: Nucleus\n\nProtein 2: SEC14L6 (B5MCN3)\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between TRIM33 and SEC14L6 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1764753, "gene_symbol_1": "TRIM33", "gene_symbol_2": "SEC14L6", "detection_method": null}} +{"question_id": "PPIL1-0913", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: CEACAM5 (P06731)\n Function: Cell surface glycoprotein that plays a role in cell adhesion, intracellular signaling and tumor progression (PubMed:10864933, PubMed:10910050, PubMed:2803308). Mediates homophilic and heterophilic cel\n Location: Cell membrane\n\nProtein 2: FAM135A (Q9P2D6)\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between CEACAM5 and FAM135A.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1881390, "gene_symbol_1": "CEACAM5", "gene_symbol_2": "FAM135A", "detection_method": null}} +{"question_id": "PPIL1-0914", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: SPEM2 (Q0P670)\n Location: Membrane\n\nProtein 2: SLC6A8 (P48029)\n Function: Creatine:sodium symporter which mediates the uptake of creatine (PubMed:17465020, PubMed:22644605, PubMed:25861866, PubMed:7945388, PubMed:7953292, PubMed:9882430). Plays an important role in supplyin\n Location: Cell membrane\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between SPEM2 and SLC6A8.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1977446, "gene_symbol_1": "SPEM2", "gene_symbol_2": "SLC6A8", "detection_method": null}} +{"question_id": "PPIL1-0915", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: CCDC106 (Q9BWC9)\n Function: Promotes the degradation of p53/TP53 protein and inhibits its transactivity\n Location: Nucleus\n\nProtein 2: DNAH7 (Q8WXX0)\n Function: Force generating protein that plays an important role in respiratory cilia and sperm flagella beating (PubMed:34476482). Produces force towards the minus ends of microtubules. Dynein has ATPase activi\n Location: Cytoplasm, cytoskeleton, cilium axoneme\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between CCDC106 and DNAH7 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2192110, "gene_symbol_1": "CCDC106", "gene_symbol_2": "DNAH7", "detection_method": null}} +{"question_id": "PPIL1-0916", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: BCOR (Q6W2J9)\n Function: Transcriptional corepressor. May specifically inhibit gene expression when recruited to promoter regions by sequence-specific DNA-binding proteins such as BCL6 and MLLT3. This repression may be mediat\n Location: Nucleus\n\nProtein 2: GSX2 (Q9BZM3)\n Function: Transcription factor that binds 5'-CNAATTAG-3' DNA sequence and regulates the expression of numerous genes including genes important for brain development (PubMed:31412107). During telencephalic devel\n Location: Nucleus\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between BCOR and GSX2.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2124180, "gene_symbol_1": "BCOR", "gene_symbol_2": "GSX2", "detection_method": null}} +{"question_id": "PPIL1-0917", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: ICMT (O60725)\n Function: Catalyzes the post-translational methylation of isoprenylated C-terminal cysteine residues\n Location: Endoplasmic reticulum membrane\n\nProtein 2: GNPTAB (Q3T906)\n Function: Catalyzes the formation of mannose 6-phosphate (M6P) markers on high mannose type oligosaccharides in the Golgi apparatus. M6P residues are required to bind to the M6P receptors (MPR), which mediate t\n Location: Golgi apparatus membrane\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between ICMT and GNPTAB is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1827248, "gene_symbol_1": "ICMT", "gene_symbol_2": "GNPTAB", "detection_method": null}} +{"question_id": "PPIL1-0918", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: MMGT1 (Q8N4V1)\n Function: Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins (PubMed:29242\n Location: Endoplasmic reticulum membrane\n\nProtein 2: PAWR (Q96IZ0)\n Function: Pro-apoptotic protein capable of selectively inducing apoptosis in cancer cells, sensitizing the cells to diverse apoptotic stimuli and causing regression of tumors in animal models. Induces apoptosis\n Location: Cytoplasm\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between MMGT1 and PAWR.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2164921, "gene_symbol_1": "MMGT1", "gene_symbol_2": "PAWR", "detection_method": null}} +{"question_id": "PPIL1-0919", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: OR14I1 (A6ND48)\n Function: Odorant receptor\n Location: Cell membrane\n\nProtein 2: EIF3E (P60228)\n Function: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773, PubMed:274628\n Location: Cytoplasm\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between OR14I1 and EIF3E.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1745235, "gene_symbol_1": "OR14I1", "gene_symbol_2": "EIF3E", "detection_method": null}} +{"question_id": "PPIL1-0920", "task": "ppi-l1", "split": "fewshot", "difficulty": "hard", "context_text": "Protein 1: TSR2 (Q969E8)\n Function: May be involved in 20S pre-rRNA processing\n\nProtein 2: DPRX (A6NFQ7)\n Function: Transcription factor that acts as a repressor\n Location: Nucleus\n\nEvidence: No significant association between TSR2 and DPRX was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1747809, "gene_symbol_1": "TSR2", "gene_symbol_2": "DPRX", "detection_method": null}} +{"question_id": "PPIL1-0921", "task": "ppi-l1", "split": "fewshot", "difficulty": "hard", "context_text": "Protein 1: MMP28 (Q9H239)\n Function: Can degrade casein. Could play a role in tissues homeostasis and repair\n Location: Secreted, extracellular space, extracellular matrix\n\nProtein 2: CHSY3 (Q70JA7)\n Function: Has both beta-1,3-glucuronic acid and beta-1,4-N-acetylgalactosamine transferase activity. Transfers glucuronic acid (GlcUA) from UDP-GlcUA and N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the no\n Location: Golgi apparatus, Golgi stack membrane\n\nEvidence: No significant association between MMP28 and CHSY3 was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2131327, "gene_symbol_1": "MMP28", "gene_symbol_2": "CHSY3", "detection_method": null}} +{"question_id": "PPIL1-0922", "task": "ppi-l1", "split": "fewshot", "difficulty": "hard", "context_text": "Protein 1: LY6G6E (A0A0B4J1T7)\n\nProtein 2: RNF145 (Q96MT1)\n Function: E3 ubiquitin ligase that catalyzes the direct transfer of ubiquitin from E2 ubiquitin-conjugating enzyme to a specific substrate. In response to bacterial infection, negatively regulates the phagocyte\n Location: Endoplasmic reticulum membrane\n\nEvidence: No significant association between LY6G6E and RNF145 was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1734086, "gene_symbol_1": "LY6G6E", "gene_symbol_2": "RNF145", "detection_method": null}} +{"question_id": "PPIL1-0923", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: DTX1 (Q86Y01)\n Function: Functions as a ubiquitin ligase protein in vivo, mediating ubiquitination and promoting degradation of MEKK1, suggesting that it may regulate the Notch pathway via some ubiquitin ligase activity (By s\n Location: Cytoplasm\n\nProtein 2: GREB1 (Q4ZG55)\n Function: May play a role in estrogen-stimulated cell proliferation. Acts as a regulator of hormone-dependent cancer growth in breast and prostate cancers\n Location: Membrane\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between DTX1 and GREB1 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2081524, "gene_symbol_1": "DTX1", "gene_symbol_2": "GREB1", "detection_method": null}} +{"question_id": "PPIL1-0924", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: CENPS-CORT (A0A087WT10)\n Location: Secreted\n\nProtein 2: COL27A1 (Q8IZC6)\n Function: Plays a role during the calcification of cartilage and the transition of cartilage to bone\n Location: Secreted, extracellular space, extracellular matrix\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between CENPS-CORT and COL27A1 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1730068, "gene_symbol_1": "CENPS-CORT", "gene_symbol_2": "COL27A1", "detection_method": null}} +{"question_id": "PPIL1-0925", "task": "ppi-l1", "split": "fewshot", "difficulty": "hard", "context_text": "Protein 1: APCS (P02743)\n Function: Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. May also function as a calcium-dependent lectin\n Location: Secreted\n\nProtein 2: RGS7 (P49802)\n Function: GTPase activator component of the RGS7-GNB5 complex that regulates G protein-coupled receptor signaling cascades (PubMed:10521509, PubMed:10862767, PubMed:31189666). The RGS7-GNB5 complex acts as an i\n Location: Cytoplasm, cytosol\n\nEvidence: No significant association between APCS and RGS7 was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1872685, "gene_symbol_1": "APCS", "gene_symbol_2": "RGS7", "detection_method": null}} +{"question_id": "PPIL1-0926", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: SNX5 (Q9Y5X3)\n Function: Involved in several stages of intracellular trafficking. Interacts with membranes containing phosphatidylinositol 3-phosphate (PtdIns(3P)) or phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4)P2) (Pub\n Location: Endosome\n\nProtein 2: PDCD7 (Q8N8D1)\n Function: Promotes apoptosis when overexpressed\n Location: Nucleus\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between SNX5 and PDCD7 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2168899, "gene_symbol_1": "SNX5", "gene_symbol_2": "PDCD7", "detection_method": null}} +{"question_id": "PPIL1-0927", "task": "ppi-l1", "split": "fewshot", "difficulty": "hard", "context_text": "Protein 1: PPM1N (Q8N819)\n\nProtein 2: OR11G2 (Q8NGC1)\n Function: Odorant receptor\n Location: Cell membrane\n\nEvidence: No significant association between PPM1N and OR11G2 was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2168583, "gene_symbol_1": "PPM1N", "gene_symbol_2": "OR11G2", "detection_method": null}} +{"question_id": "PPIL1-0928", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: NOTCH4 (Q99466)\n Function: Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it for\n Location: Cell membrane\n\nProtein 2: FPR3 (P25089)\n Function: May function as a pattern recognition G-protein coupled receptor (PRR/GPCR) involved in innate recognition of peptides derived from a specific set of bacterial pathogens or host mitochondria as pathog\n Location: Cell membrane\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between NOTCH4 and FPR3.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1933328, "gene_symbol_1": "NOTCH4", "gene_symbol_2": "FPR3", "detection_method": null}} +{"question_id": "PPIL1-0929", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: BRMS1L (Q5PSV4)\n Function: Involved in the histone deacetylase (HDAC1)-dependent transcriptional repression activity. When overexpressed in lung cancer cell line that lacks p53/TP53 expression, inhibits cell growth\n Location: Nucleus\n\nProtein 2: ATPAF1 (Q5TC12)\n Function: Has a complex stabilizing activity in the assembly of the mitochondrial F1-F0 complex\n Location: Mitochondrion inner membrane\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between BRMS1L and ATPAF1.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2090805, "gene_symbol_1": "BRMS1L", "gene_symbol_2": "ATPAF1", "detection_method": null}} +{"question_id": "PPIL1-0930", "task": "ppi-l1", "split": "fewshot", "difficulty": "hard", "context_text": "Protein 1: NANOS3 (P60323)\n Function: Plays a role in the maintenance of the undifferentiated state of germ cells regulating the spermatogonia cell cycle and inducing a prolonged transit in G1 phase. Affects cell proliferation probably by\n Location: Nucleus\n\nProtein 2: TMEM208 (Q9BTX3)\n Function: May function as a negative regulator of endoplasmic reticulum-stress induced autophagy\n Location: Endoplasmic reticulum membrane\n\nEvidence: No significant association between NANOS3 and TMEM208 was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2005525, "gene_symbol_1": "NANOS3", "gene_symbol_2": "TMEM208", "detection_method": null}} +{"question_id": "PPIL1-0931", "task": "ppi-l1", "split": "fewshot", "difficulty": "hard", "context_text": "Protein 1: OIT3 (Q8WWZ8)\n Function: May be involved in hepatocellular function and development\n Location: Nucleus envelope\n\nProtein 2: NDUFA12 (Q9UI09)\n Function: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons fro\n Location: Mitochondrion inner membrane\n\nEvidence: No significant association between OIT3 and NDUFA12 was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2191211, "gene_symbol_1": "OIT3", "gene_symbol_2": "NDUFA12", "detection_method": null}} +{"question_id": "PPIL1-0932", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: ARL11 (Q969Q4)\n Function: May play a role in apoptosis. May act as a tumor suppressor\n\nProtein 2: EXO5 (Q9H790)\n Function: Single-stranded DNA (ssDNA) bidirectional exonuclease involved in DNA repair. Probably involved in DNA repair following ultraviolet (UV) irradiation and interstrand cross-links (ICLs) damage. Has both\n Location: Nucleus\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between ARL11 and EXO5 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2197508, "gene_symbol_1": "ARL11", "gene_symbol_2": "EXO5", "detection_method": null}} +{"question_id": "PPIL1-0933", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: GRP (P07492)\n Function: Stimulates the release of gastrin and other gastrointestinal hormones (By similarity). Contributes to the perception of prurient stimuli and to the transmission of itch signals in the spinal cord that\n Location: Secreted\n\nProtein 2: ABCC3 (O15438)\n Function: ATP-dependent transporter of the ATP-binding cassette (ABC) family that binds and hydrolyzes ATP to enable active transport of various substrates including many drugs, toxicants and endogenous compoun\n Location: Basolateral cell membrane\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between GRP and ABCC3.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1806491, "gene_symbol_1": "GRP", "gene_symbol_2": "ABCC3", "detection_method": null}} +{"question_id": "PPIL1-0934", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: ACAP1 (Q15027)\n Function: GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface. Required for r\n Location: Recycling endosome membrane\n\nProtein 2: ADGRG2 (Q8IZP9)\n Function: Adhesion G-protein coupled receptor (aGPCR) for steroid hormones, such as dehydroepiandrosterone (DHEA; also named 3beta-hydroxyandrost-5-en-17-one) and androstenedione (PubMed:29393851, PubMed:359822\n Location: Apical cell membrane\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between ACAP1 and ADGRG2 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2056793, "gene_symbol_1": "ACAP1", "gene_symbol_2": "ADGRG2", "detection_method": null}} +{"question_id": "PPIL1-0935", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: CXCR6 (O00574)\n Function: Receptor for the C-X-C chemokine CXCL16. Used as a coreceptor by SIVs and by strains of HIV-2 and m-tropic HIV-1\n Location: Cell membrane\n\nProtein 2: GDPGP1 (Q6ZNW5)\n Function: Specific and highly efficient GDP-D-glucose phosphorylase regulating the levels of GDP-D-glucose in cells (PubMed:21507950). Is involved in adaptive response to stress conditions, and plays a key role\n Location: Cytoplasm\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between CXCR6 and GDPGP1.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1789524, "gene_symbol_1": "CXCR6", "gene_symbol_2": "GDPGP1", "detection_method": null}} +{"question_id": "PPIL1-0936", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: DEFB135 (Q30KP9)\n Function: Has antibacterial activity\n Location: Secreted\n\nProtein 2: RAB11FIP3 (O75154)\n Function: Downstream effector molecule for Rab11 GTPase which is involved in endocytic trafficking, cytokinesis and intracellular ciliogenesis by participating in membrane delivery (PubMed:15601896, PubMed:1614\n Location: Endosome membrane\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between DEFB135 and RAB11FIP3 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1832675, "gene_symbol_1": "DEFB135", "gene_symbol_2": "RAB11FIP3", "detection_method": null}} +{"question_id": "PPIL1-0937", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: ID1 (P41134)\n Function: Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA bindi\n Location: Cytoplasm\n\nProtein 2: SLC30A6 (Q6NXT4)\n Function: Has probably no intrinsic transporter activity but together with SLC30A5 forms a functional zinc ion:proton antiporter heterodimer, mediating zinc entry into the lumen of organelles along the secretor\n Location: Golgi apparatus, trans-Golgi network membrane\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between ID1 and SLC30A6 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1967958, "gene_symbol_1": "ID1", "gene_symbol_2": "SLC30A6", "detection_method": null}} +{"question_id": "PPIL1-0938", "task": "ppi-l1", "split": "fewshot", "difficulty": "hard", "context_text": "Protein 1: STAT3 (P40763)\n Function: Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors (PubMed:10688651, PubMed:12359225, PubMed:12873986, PubMed:15194\n Location: Cytoplasm\n\nProtein 2: PRAMEF20 (Q5VT98)\n\nEvidence: No significant association between STAT3 and PRAMEF20 was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1967455, "gene_symbol_1": "STAT3", "gene_symbol_2": "PRAMEF20", "detection_method": null}} +{"question_id": "PPIL1-0939", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: TRAF1 (Q13077)\n Function: Adapter molecule that regulates the activation of NF-kappa-B and JNK. Plays a role in the regulation of cell survival and apoptosis. The heterotrimer formed by TRAF1 and TRAF2 is part of a E3 ubiquiti\n\nProtein 2: XKR7 (Q5GH72)\n Location: Cell membrane\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between TRAF1 and XKR7.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2038439, "gene_symbol_1": "TRAF1", "gene_symbol_2": "XKR7", "detection_method": null}} +{"question_id": "PPIL1-0940", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: DLX4 (Q92988)\n Function: May play a role in determining the production of hemoglobin S. May act as a repressor. During embryonic development, plays a role in palatogenesis\n Location: Nucleus\n\nProtein 2: TULP1 (O00294)\n Function: Required for normal development of photoreceptor synapses. Required for normal photoreceptor function and for long-term survival of photoreceptor cells. Interacts with cytoskeleton proteins and may pl\n Location: Cytoplasm\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between DLX4 and TULP1.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1785522, "gene_symbol_1": "DLX4", "gene_symbol_2": "TULP1", "detection_method": null}} +{"question_id": "PPIL1-0941", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: MORN3 (Q6PF18)\n Function: Assembles a suppression complex (suppresome) by tethering SIRT1 and MDM2 to regulate composite modifications of p53/TP53. Confers both deacetylation-mediated functional inactivation, by SIRT1, and ubi\n Location: Cytoplasmic vesicle, secretory vesicle, acrosome\n\nProtein 2: CHCHD6 (Q9BRQ6)\n Function: Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation \n Location: Mitochondrion inner membrane\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between MORN3 and CHCHD6.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2116943, "gene_symbol_1": "MORN3", "gene_symbol_2": "CHCHD6", "detection_method": null}} +{"question_id": "PPIL1-0942", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: KAT7 (O95251)\n Function: Catalytic subunit of histone acetyltransferase HBO1 complexes, which specifically mediate acetylation of histone H3 at 'Lys-14' (H3K14ac), thereby regulating various processes, such as gene transcript\n Location: Nucleus\n\nProtein 2: ORAI2 (Q96SN7)\n Function: Pore-forming subunit of inward rectifying Ca(2+) release-activated Ca(2+) (CRAC) channels. Assembles with ORAI1 and ORAI3 to form hexameric CRAC channels that mediate Ca(2+) influx upon depletion of e\n Location: Cell membrane\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between KAT7 and ORAI2 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1854882, "gene_symbol_1": "KAT7", "gene_symbol_2": "ORAI2", "detection_method": null}} +{"question_id": "PPIL1-0943", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: KCTD13 (Q8WZ19)\n Function: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex required for synaptic transmission (PubMed:19782033). The BCR(KCTD13) E3 ubiquitin ligase complex mediates the u\n Location: Nucleus\n\nProtein 2: CCDC126 (Q96EE4)\n Location: Secreted\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between KCTD13 and CCDC126.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2192636, "gene_symbol_1": "KCTD13", "gene_symbol_2": "CCDC126", "detection_method": null}} +{"question_id": "PPIL1-0944", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: ANK1 (P16157)\n Function: Component of the ankyrin-1 complex, a multiprotein complex involved in the stability and shape of the erythrocyte membrane (PubMed:35835865). Attaches integral membrane proteins to cytoskeletal elemen\n Location: Cytoplasm, cytoskeleton\n\nProtein 2: ROMO1 (P60602)\n Function: Induces production of reactive oxygen species (ROS) which are necessary for cell proliferation. May play a role in inducing oxidative DNA damage and replicative senescence. May play a role in the coor\n Location: Mitochondrion inner membrane\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between ANK1 and ROMO1 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1913267, "gene_symbol_1": "ANK1", "gene_symbol_2": "ROMO1", "detection_method": null}} +{"question_id": "PPIL1-0945", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: SHISAL1 (Q3SXP7)\n Location: Membrane\n\nProtein 2: H3BML4 (H3BML4)\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between SHISAL1 and None.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1776061, "gene_symbol_1": "SHISAL1", "gene_symbol_2": null, "detection_method": null}} +{"question_id": "PPIL1-0946", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: MAPK8IP2 (Q13387)\n Function: The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. JIP2 i\n Location: Cytoplasm\n\nProtein 2: CCL8 (P80075)\n Function: Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. May play a role in neoplasia and inflammatory host responses. This protein can bind heparin. The processed form MCP-\n Location: Secreted\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between MAPK8IP2 and CCL8 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2015682, "gene_symbol_1": "MAPK8IP2", "gene_symbol_2": "CCL8", "detection_method": null}} +{"question_id": "PPIL1-0947", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: ZNF641 (Q96N77)\n Function: Transcriptional activator. Activates transcriptional activities of SRE and AP-1\n Location: Nucleus\n\nProtein 2: GRM3 (Q14832)\n Function: G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-st\n Location: Cell membrane\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between ZNF641 and GRM3.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2054717, "gene_symbol_1": "ZNF641", "gene_symbol_2": "GRM3", "detection_method": null}} +{"question_id": "PPIL1-0948", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: HAPSTR1 (Q14CZ0)\n Function: Acts as a central player within a network of stress response pathways promoting cellular adaptability. The E3 ligase HUWE1 assists HAPSTR1 in controlling stress signaling and in turn, HUWE1 feeds back\n Location: Nucleus\n\nProtein 2: ZNF215 (Q9UL58)\n Function: May be involved in transcriptional regulation\n Location: Nucleus\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between HAPSTR1 and ZNF215 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2056073, "gene_symbol_1": "HAPSTR1", "gene_symbol_2": "ZNF215", "detection_method": null}} +{"question_id": "PPIL1-0949", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: TMEM165 (Q9HC07)\n Function: Putative divalent cation:proton antiporter that exchanges calcium or manganese ions for protons across the Golgi membrane. Mediates the reversible transport of calcium or manganese to the Golgi lumen \n Location: Golgi apparatus membrane\n\nProtein 2: TNFRSF1A (P19438)\n Function: Receptor for TNFSF2/TNF and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) perform\n Location: Cell membrane\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between TMEM165 and TNFRSF1A.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1920627, "gene_symbol_1": "TMEM165", "gene_symbol_2": "TNFRSF1A", "detection_method": null}} +{"question_id": "PPIL1-0950", "task": "ppi-l1", "split": "fewshot", "difficulty": "hard", "context_text": "Protein 1: VPS11 (Q9H270)\n Function: Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act as a core component of the putative H\n Location: Endosome\n\nProtein 2: CHTF8 (P0CG13)\n Function: Chromosome cohesion factor involved in sister chromatid cohesion and fidelity of chromosome transmission. Component of one of the cell nuclear antigen loader complexes, CTF18-replication factor C (CTF\n Location: Nucleus\n\nEvidence: No significant association between VPS11 and CHTF8 was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1894082, "gene_symbol_1": "VPS11", "gene_symbol_2": "CHTF8", "detection_method": null}} +{"question_id": "PPIL1-0951", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: KDELR1 (P24390)\n Function: Receptor for the C-terminal sequence motif K-D-E-L that is present on endoplasmic reticulum resident proteins and that mediates their recycling from the Golgi back to the endoplasmic reticulum\n Location: Golgi apparatus membrane\n\nProtein 2: DYDC1 (Q8WWB3)\n Function: Functions as part of axonemal radial spoke complexes that play an important part in the motility of sperm and cilia (By similarity). Plays a crucial role during acrosome biogenesis (PubMed:19545932)\n Location: Cytoplasm, cytoskeleton, flagellum axoneme\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between KDELR1 and DYDC1 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1932179, "gene_symbol_1": "KDELR1", "gene_symbol_2": "DYDC1", "detection_method": null}} +{"question_id": "PPIL1-0952", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: GRIP1 (Q9Y3R0)\n Function: May play a role as a localized scaffold for the assembly of a multiprotein signaling complex and as mediator of the trafficking of its binding partners at specific subcellular location in neurons (Pub\n Location: Cytoplasmic vesicle\n\nProtein 2: IGFL3 (Q6UXB1)\n Function: Potential ligand of the IGFLR1 cell membrane receptor\n Location: Secreted\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between GRIP1 and IGFL3.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2122490, "gene_symbol_1": "GRIP1", "gene_symbol_2": "IGFL3", "detection_method": null}} +{"question_id": "PPIL1-0953", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: GLRX3 (O76003)\n Function: Together with BOLA2, acts as a cytosolic iron-sulfur (Fe-S) cluster assembly factor that facilitates [2Fe-2S] cluster insertion into a subset of cytosolic proteins (PubMed:26613676, PubMed:27519415). \n Location: Cytoplasm, cytosol\n\nProtein 2: NEFM (P07197)\n Function: Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal interm\n Location: Cytoplasm, cytoskeleton\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between GLRX3 and NEFM.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1845524, "gene_symbol_1": "GLRX3", "gene_symbol_2": "NEFM", "detection_method": null}} +{"question_id": "PPIL1-0954", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: TTLL6 (Q8N841)\n Function: Polyglutamylase which modifies both tubulin and non-tubulin proteins, generating alpha-linked polyglutamate side chains on the gamma-carboxyl group of specific glutamate residues of target proteins. P\n Location: Cytoplasm\n\nProtein 2: MUC21 (Q5SSG8)\n Location: Cell membrane\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between TTLL6 and MUC21 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2091992, "gene_symbol_1": "TTLL6", "gene_symbol_2": "MUC21", "detection_method": null}} +{"question_id": "PPIL1-0955", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: ELOVL6 (Q9H5J4)\n Function: Catalyzes the first and rate-limiting reaction of the four reactions that constitute the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process allows the addition\n Location: Endoplasmic reticulum membrane\n\nProtein 2: ACTRT3 (Q9BYD9)\n Location: Cytoplasm, cytoskeleton\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between ELOVL6 and ACTRT3 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2222089, "gene_symbol_1": "ELOVL6", "gene_symbol_2": "ACTRT3", "detection_method": null}} +{"question_id": "PPIL1-0956", "task": "ppi-l1", "split": "fewshot", "difficulty": "easy", "context_text": "Protein 1: PLEKHO2 (Q8TD55)\n\nProtein 2: CHRND (Q07001)\n Function: After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane\n Location: Postsynaptic cell membrane\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between PLEKHO2 and CHRND is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2029135, "gene_symbol_1": "PLEKHO2", "gene_symbol_2": "CHRND", "detection_method": null}} +{"question_id": "PPIL1-0957", "task": "ppi-l1", "split": "fewshot", "difficulty": "hard", "context_text": "Protein 1: C1GALT1 (Q9NS00)\n Function: Glycosyltransferase that generates the core 1 O-glycan Gal-beta1-3GalNAc-alpha1-Ser/Thr (T antigen), which is a precursor for many extended O-glycans in glycoproteins (PubMed:11677243). Plays a centra\n Location: Membrane\n\nProtein 2: MTOR (P42345)\n Function: Serine/threonine protein kinase which is a central regulator of cellular metabolism, growth and survival in response to hormones, growth factors, nutrients, energy and stress signals (PubMed:12087098,\n Location: Lysosome membrane\n\nEvidence: No significant association between C1GALT1 and MTOR was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1970417, "gene_symbol_1": "C1GALT1", "gene_symbol_2": "MTOR", "detection_method": null}} +{"question_id": "PPIL1-0958", "task": "ppi-l1", "split": "fewshot", "difficulty": "hard", "context_text": "Protein 1: KRTAP5-3 (Q6L8H2)\n Function: In the hair cortex, hair keratin intermediate filaments are embedded in an interfilamentous matrix, consisting of hair keratin-associated protein (KRTAP), which are essential for the formation of a ri\n\nProtein 2: CATIP (Q7Z7H3)\n Function: Plays a role in primary ciliogenesis by modulating actin polymerization\n Location: Nucleus\n\nEvidence: No significant association between KRTAP5-3 and CATIP was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2111062, "gene_symbol_1": "KRTAP5-3", "gene_symbol_2": "CATIP", "detection_method": null}} +{"question_id": "PPIL1-0959", "task": "ppi-l1", "split": "fewshot", "difficulty": "medium", "context_text": "Protein 1: RABGAP1 (Q9Y3P9)\n Function: May act as a GTPase-activating protein of RAB6A. May play a role in microtubule nucleation by centrosome. May participate in a RAB6A-mediated pathway involved in the metaphase-anaphase transition\n Location: Cytoplasm, cytosol\n\nProtein 2: CADPS2 (Q86UW7)\n Function: Calcium-binding protein involved in exocytosis of vesicles filled with neurotransmitters and neuropeptides. Probably acts upstream of fusion in the biogenesis or maintenance of mature secretory vesicl\n Location: Cytoplasmic vesicle membrane\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between RABGAP1 and CADPS2.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2144167, "gene_symbol_1": "RABGAP1", "gene_symbol_2": "CADPS2", "detection_method": null}} +{"question_id": "PPIL1-0960", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: PAK1IP1 (Q9NWT1)\n Function: Negatively regulates the PAK1 kinase. PAK1 is a member of the PAK kinase family, which has been shown to play a positive role in the regulation of signaling pathways involving MAPK8 and RELA. PAK1 exi\n Location: Nucleus, nucleolus\n\nProtein 2: RNF11 (Q9Y3C5)\n Function: Essential component of a ubiquitin-editing protein complex, comprising also TNFAIP3, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. Promotes the association of\n Location: Early endosome\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between PAK1IP1 and RNF11 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2234601, "gene_symbol_1": "PAK1IP1", "gene_symbol_2": "RNF11", "detection_method": null}} +{"question_id": "PPIL1-0961", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: DCTN4 (Q9UJW0)\n Function: Part of the dynactin complex that activates the molecular motor dynein for ultra-processive transport along microtubules\n Location: Cytoplasm, cytoskeleton\n\nProtein 2: MTIF3 (Q9H2K0)\n Function: IF-3 binds to the 28S ribosomal subunit and shifts the equilibrium between 55S ribosomes and their 39S and 28S subunits in favor of the free subunits, thus enhancing the availability of 28S subunits o\n Location: Mitochondrion\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between DCTN4 and MTIF3 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2226574, "gene_symbol_1": "DCTN4", "gene_symbol_2": "MTIF3", "detection_method": null}} +{"question_id": "PPIL1-0962", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: RASGRP2 (Q7LDG7)\n Function: Functions as a calcium- and DAG-regulated nucleotide exchange factor specifically activating Rap through the exchange of bound GDP for GTP. May also activate other GTPases such as RRAS, RRAS2, NRAS, K\n Location: Cytoplasm, cytosol\n\nProtein 2: MAGEB3 (O15480)\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between RASGRP2 and MAGEB3 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1807021, "gene_symbol_1": "RASGRP2", "gene_symbol_2": "MAGEB3", "detection_method": null}} +{"question_id": "PPIL1-0963", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: MSL2 (Q9HCI7)\n Function: Non-catalytic component of the MSL histone acetyltransferase complex, a multiprotein complex that mediates the majority of histone H4 acetylation at 'Lys-16' (H4K16ac), an epigenetic mark that prevent\n Location: Nucleus\n\nProtein 2: LRRC28 (Q86X40)\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between MSL2 and LRRC28.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2147492, "gene_symbol_1": "MSL2", "gene_symbol_2": "LRRC28", "detection_method": null}} +{"question_id": "PPIL1-0964", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: SPARCL1 (Q14515)\n Location: Secreted, extracellular space, extracellular matrix\n\nProtein 2: IL5 (P05113)\n Function: Homodimeric cytokine expressed predominantly by T-lymphocytes and NK cells that plays an important role in the survival, differentiation, and chemotaxis of eosinophils (PubMed:2653458, PubMed:9010276)\n Location: Secreted\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between SPARCL1 and IL5 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1878391, "gene_symbol_1": "SPARCL1", "gene_symbol_2": "IL5", "detection_method": null}} +{"question_id": "PPIL1-0965", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: L3MBTL4 (Q8NA19)\n Function: Putative Polycomb group (PcG) protein. PcG proteins maintain the transcriptionally repressive state of genes, probably via a modification of chromatin, rendering it heritably changed in its expressibi\n Location: Nucleus\n\nProtein 2: CERS2 (Q96G23)\n Function: Ceramide synthase that catalyzes the transfer of the acyl chain from acyl-CoA to a sphingoid base, with high selectivity toward very-long-chain fatty acyl-CoA (chain length C22-C27) (PubMed:17977534, \n Location: Endoplasmic reticulum membrane\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between L3MBTL4 and CERS2 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2170720, "gene_symbol_1": "L3MBTL4", "gene_symbol_2": "CERS2", "detection_method": null}} +{"question_id": "PPIL1-0966", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: CCT4 (P50991)\n Function: Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of actin, tubulin and other proteins upon ATP hydrolysis (PubMed:25467444, PubMed:364937\n Location: Cytoplasm\n\nProtein 2: SPINK7 (P58062)\n Function: Probable serine protease inhibitor\n Location: Secreted\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between CCT4 and SPINK7.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1986617, "gene_symbol_1": "CCT4", "gene_symbol_2": "SPINK7", "detection_method": null}} +{"question_id": "PPIL1-0967", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: HSP90B1 (P14625)\n Function: ATP-dependent chaperone involved in the processing of proteins in the endoplasmic reticulum, regulating their transport (PubMed:23572575, PubMed:39509507). Together with MESD, acts as a modulator of t\n Location: Endoplasmic reticulum lumen\n\nProtein 2: TTBK2 (Q6IQ55)\n Function: Serine/threonine kinase that acts as a key regulator of ciliogenesis: controls the initiation of ciliogenesis by binding to the distal end of the basal body and promoting the removal of CCP110, which \n Location: Cell projection, cilium\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between HSP90B1 and TTBK2 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1909215, "gene_symbol_1": "HSP90B1", "gene_symbol_2": "TTBK2", "detection_method": null}} +{"question_id": "PPIL1-0968", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: ZNF790 (Q6PG37)\n Function: May be involved in transcriptional regulation\n Location: Nucleus\n\nProtein 2: PAIP2B (Q9ULR5)\n Function: Inhibits translation of capped and polyadenylated mRNAs by displacing PABPC1 from the poly(A) tail\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between ZNF790 and PAIP2B.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2117022, "gene_symbol_1": "ZNF790", "gene_symbol_2": "PAIP2B", "detection_method": null}} +{"question_id": "PPIL1-0969", "task": "ppi-l1", "split": "val", "difficulty": "hard", "context_text": "Protein 1: HLA-DMB (P28068)\n Function: Plays a critical role in catalyzing the release of class II-associated invariant chain peptide (CLIP) from newly synthesized MHC class II molecules and freeing the peptide binding site for acquisition\n Location: Late endosome membrane\n\nProtein 2: SLC6A7 (Q99884)\n Function: Brain specific sodium (and chloride)-dependent proline transporter (PubMed:7651355). Terminates the action of proline by its high affinity sodium-dependent reuptake into presynaptic terminals (Probabl\n Location: Synaptic cell membrane\n\nEvidence: No significant association between HLA-DMB and SLC6A7 was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1937578, "gene_symbol_1": "HLA-DMB", "gene_symbol_2": "SLC6A7", "detection_method": null}} +{"question_id": "PPIL1-0970", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: ADH7 (P40394)\n Function: Catalyzes the NAD-dependent oxidation of all-trans-retinol, alcohol, and omega-hydroxy fatty acids and their derivatives (PubMed:15369820, PubMed:16787387, PubMed:9600267). Oxidizes preferentially all\n Location: Cytoplasm\n\nProtein 2: TNFAIP8L2 (Q6P589)\n Function: Acts as a negative regulator of innate and adaptive immunity by maintaining immune homeostasis (PubMed:27043859). Plays a regulatory role in the Toll-like signaling pathway by determining the strength\n Location: Cytoplasm\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between ADH7 and TNFAIP8L2 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1967132, "gene_symbol_1": "ADH7", "gene_symbol_2": "TNFAIP8L2", "detection_method": null}} +{"question_id": "PPIL1-0971", "task": "ppi-l1", "split": "val", "difficulty": "hard", "context_text": "Protein 1: MDFI (Q99750)\n Function: Inhibits the transactivation activity of the Myod family of myogenic factors and represses myogenesis (By similarity). Acts by associating with Myod family members and retaining them in the cytoplasm \n Location: Nucleus\n\nProtein 2: CAVIN3 (Q969G5)\n Function: Regulates the traffic and/or budding of caveolae (PubMed:19262564). Plays a role in caveola formation in a tissue-specific manner. Required for the formation of caveolae in smooth muscle but not in th\n Location: Cytoplasm\n\nEvidence: No significant association between MDFI and CAVIN3 was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2197033, "gene_symbol_1": "MDFI", "gene_symbol_2": "CAVIN3", "detection_method": null}} +{"question_id": "PPIL1-0972", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: SIVA1 (O15304)\n Function: Induces CD27-mediated apoptosis. Inhibits BCL2L1 isoform Bcl-x(L) anti-apoptotic activity. Inhibits activation of NF-kappa-B and promotes T-cell receptor-mediated apoptosis\n Location: Cytoplasm\n\nProtein 2: NEU4 (Q8WWR8)\n Function: Exo-alpha-sialidase that catalyzes the hydrolytic cleavage of the terminal sialic acid (N-acetylneuraminic acid, Neu5Ac) of a glycan moiety in the catabolism of glycolipids, glycoproteins and oligosac\n Location: Cell membrane\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between SIVA1 and NEU4 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1804790, "gene_symbol_1": "SIVA1", "gene_symbol_2": "NEU4", "detection_method": null}} +{"question_id": "PPIL1-0973", "task": "ppi-l1", "split": "val", "difficulty": "hard", "context_text": "Protein 1: LY6G6E (A0A0B4J1T7)\n\nProtein 2: MAPKAPK3 (Q16644)\n Function: Stress-activated serine/threonine-protein kinase involved in cytokines production, endocytosis, cell migration, chromatin remodeling and transcriptional regulation. Following stress, it is phosphoryla\n Location: Nucleus\n\nEvidence: No significant association between LY6G6E and MAPKAPK3 was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1734068, "gene_symbol_1": "LY6G6E", "gene_symbol_2": "MAPKAPK3", "detection_method": null}} +{"question_id": "PPIL1-0974", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: ZNF135 (P52742)\n Function: Plays a role in the regulation of cell morphology and cytoskeletal organization. May be involved in transcriptional regulation\n Location: Nucleus\n\nProtein 2: MEAF6 (Q9HAF1)\n Function: Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A (PubMed:14966270). \n Location: Nucleus, nucleolus\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between ZNF135 and MEAF6.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1992041, "gene_symbol_1": "ZNF135", "gene_symbol_2": "MEAF6", "detection_method": null}} +{"question_id": "PPIL1-0975", "task": "ppi-l1", "split": "val", "difficulty": "hard", "context_text": "Protein 1: NUP37 (Q8NFH4)\n Function: Component of the Nup107-160 subcomplex of the nuclear pore complex (NPC). The Nup107-160 subcomplex is required for the assembly of a functional NPC. The Nup107-160 subcomplex is also required for nor\n Location: Chromosome, centromere, kinetochore\n\nProtein 2: PHF23 (Q9BUL5)\n Function: Acts as a negative regulator of autophagy, through promoting ubiquitination and degradation of LRSAM1, an E3 ubiquitin ligase that promotes autophagy in response to starvation or infecting bacteria\n Location: Nucleus\n\nEvidence: No significant association between NUP37 and PHF23 was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2176665, "gene_symbol_1": "NUP37", "gene_symbol_2": "PHF23", "detection_method": null}} +{"question_id": "PPIL1-0976", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: ZNF682 (O95780)\n Function: May be involved in transcriptional regulation\n Location: Nucleus\n\nProtein 2: GCG (P01275)\n Function: Plays a key role in glucose metabolism and homeostasis. Regulates blood glucose by increasing gluconeogenesis and decreasing glycolysis. A counterregulatory hormone of insulin, raises plasma glucose l\n Location: Secreted\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between ZNF682 and GCG is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1861668, "gene_symbol_1": "ZNF682", "gene_symbol_2": "GCG", "detection_method": null}} +{"question_id": "PPIL1-0977", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: DYRK4 (Q9NR20)\n Function: Possible non-essential role in spermiogenesis\n Location: Cytoplasm\n\nProtein 2: PRCP (P42785)\n Function: Cleaves C-terminal amino acids linked to proline in peptides such as angiotensin II, III and des-Arg9-bradykinin. This cleavage occurs at acidic pH, but enzymatic activity is retained with some substr\n Location: Lysosome\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between DYRK4 and PRCP.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1971429, "gene_symbol_1": "DYRK4", "gene_symbol_2": "PRCP", "detection_method": null}} +{"question_id": "PPIL1-0978", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: SUPV3L1 (Q8IYB8)\n Function: Major helicase player in mitochondrial RNA metabolism. Component of the mitochondrial degradosome (mtEXO) complex, that degrades 3' overhang double-stranded RNA with a 3'-to-5' directionality in an AT\n Location: Nucleus\n\nProtein 2: NDUFAF2 (Q8N183)\n Function: Acts as a molecular chaperone for mitochondrial complex I assembly (PubMed:16200211, PubMed:19384974). Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediat\n Location: Mitochondrion\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between SUPV3L1 and NDUFAF2.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2156544, "gene_symbol_1": "SUPV3L1", "gene_symbol_2": "NDUFAF2", "detection_method": null}} +{"question_id": "PPIL1-0979", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: ADAM22 (Q9P0K1)\n Function: Probable ligand for integrin in the brain. This is a non catalytic metalloprotease-like protein (PubMed:19692335). Involved in regulation of cell adhesion and spreading and in inhibition of cell proli\n Location: Cell membrane\n\nProtein 2: CDC42BPB (Q9Y5S2)\n Function: Serine/threonine-protein kinase which is an important downstream effector of CDC42 and plays a role in the regulation of cytoskeleton reorganization and cell migration. Regulates actin cytoskeletal re\n Location: Cytoplasm\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between ADAM22 and CDC42BPB is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2236015, "gene_symbol_1": "ADAM22", "gene_symbol_2": "CDC42BPB", "detection_method": null}} +{"question_id": "PPIL1-0980", "task": "ppi-l1", "split": "val", "difficulty": "hard", "context_text": "Protein 1: SLC15A4 (Q8N697)\n Function: Proton-coupled amino-acid transporter that mediates the transmembrane transport of L-histidine and some di- and tripeptides from inside the lysosome to the cytosol, and plays a key role in innate immu\n Location: Lysosome membrane\n\nProtein 2: SLC12A3 (P55017)\n Function: Electroneutral sodium and chloride ion cotransporter, which acts as a key mediator of sodium and chloride reabsorption in kidney distal convoluted tubules (PubMed:18270262, PubMed:21613606, PubMed:220\n Location: Cell membrane\n\nEvidence: No significant association between SLC15A4 and SLC12A3 was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1996940, "gene_symbol_1": "SLC15A4", "gene_symbol_2": "SLC12A3", "detection_method": null}} +{"question_id": "PPIL1-0981", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: AKAP8L (Q9ULX6)\n Function: Could play a role in constitutive transport element (CTE)-mediated gene expression by association with DHX9. Increases CTE-dependent nuclear unspliced mRNA export (PubMed:10748171, PubMed:11402034). P\n Location: Nucleus\n\nProtein 2: TMEM170A (Q8WVE7)\n Function: Acts as a regulator of endoplasmic reticulum (ER) and nuclear envelope (NE) morphogenesis. Affects the ratio between tubular ER and ER sheets by promoting sheet formation at the expense of tubules. In\n Location: Endoplasmic reticulum membrane\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between AKAP8L and TMEM170A is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2189559, "gene_symbol_1": "AKAP8L", "gene_symbol_2": "TMEM170A", "detection_method": null}} +{"question_id": "PPIL1-0982", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: POLB (P06746)\n Function: Repair polymerase that plays a key role in base-excision repair (PubMed:10556592, PubMed:9207062, PubMed:9572863). During this process, the damaged base is excised by specific DNA glycosylases, the DN\n Location: Nucleus\n\nProtein 2: ZNF648 (Q5T619)\n Function: May be involved in transcriptional regulation\n Location: Nucleus\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between POLB and ZNF648 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1881580, "gene_symbol_1": "POLB", "gene_symbol_2": "ZNF648", "detection_method": null}} +{"question_id": "PPIL1-0983", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: DACH1 (Q9UI36)\n Function: Transcription factor that is involved in regulation of organogenesis. Seems to be a regulator of SIX1, SIX6 and probably SIX5. Corepression of precursor cell proliferation in myoblasts by SIX1 is swit\n Location: Nucleus\n\nProtein 2: COL19A1 (Q14993)\n Function: May act as a cross-bridge between fibrils and other extracellular matrix molecules. Involved in skeletal myogenesis in the developing esophagus. May play a role in organization of the pericellular mat\n Location: Secreted, extracellular space, extracellular matrix\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between DACH1 and COL19A1.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2055585, "gene_symbol_1": "DACH1", "gene_symbol_2": "COL19A1", "detection_method": null}} +{"question_id": "PPIL1-0984", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: AXIN1 (O15169)\n Function: Component of the beta-catenin destruction complex required for regulating CTNNB1 levels through phosphorylation and ubiquitination, and modulating Wnt-signaling (PubMed:12192039, PubMed:27098453, PubM\n Location: Cytoplasm\n\nProtein 2: NQO2 (P16083)\n Function: The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinones involved in detoxification pathways as well as in biosynthetic processes such as the vitam\n Location: Cytoplasm\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between AXIN1 and NQO2 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1802501, "gene_symbol_1": "AXIN1", "gene_symbol_2": "NQO2", "detection_method": null}} +{"question_id": "PPIL1-0985", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: NXT1 (Q9UKK6)\n Function: Stimulator of protein export for NES-containing proteins (PubMed:10567585). Also plays a role in the nuclear export of U1 snRNA, tRNA, and mRNA (PubMed:10848583). The NXF1-NXT1 heterodimer is involved\n Location: Nucleus\n\nProtein 2: CHTF8 (P0CG13)\n Function: Chromosome cohesion factor involved in sister chromatid cohesion and fidelity of chromosome transmission. Component of one of the cell nuclear antigen loader complexes, CTF18-replication factor C (CTF\n Location: Nucleus\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between NXT1 and CHTF8 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1894086, "gene_symbol_1": "NXT1", "gene_symbol_2": "CHTF8", "detection_method": null}} +{"question_id": "PPIL1-0986", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: CEP192 (Q8TEP8)\n Function: Required for mitotic centrosome maturation and bipolar spindle assembly (PubMed:17980596, PubMed:18207742, PubMed:25042804). Appears to be a major regulator of pericentriolar material (PCM) recruitmen\n Location: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole\n\nProtein 2: TOPAZ1 (Q8N9V7)\n Function: Important for normal spermatogenesis and male fertility. Specifically required for progression to the post-meiotic stages of spermatocyte development. Seems to be necessary for normal expression level\n Location: Cytoplasm, cytosol\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between CEP192 and TOPAZ1.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2170482, "gene_symbol_1": "CEP192", "gene_symbol_2": "TOPAZ1", "detection_method": null}} +{"question_id": "PPIL1-0987", "task": "ppi-l1", "split": "val", "difficulty": "hard", "context_text": "Protein 1: CEP89 (Q96ST8)\n Function: Required for ciliogenesis. Also plays a role in mitochondrial metabolism where it may modulate complex IV activity\n Location: Cytoplasm, cytosol\n\nProtein 2: BACH1 (O14867)\n Function: Transcriptional regulator that acts as a repressor or activator, depending on the context. Binds to NF-E2 DNA binding sites. Plays important roles in coordinating transcription activation and repressi\n Location: Nucleus\n\nEvidence: No significant association between CEP89 and BACH1 was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1797328, "gene_symbol_1": "CEP89", "gene_symbol_2": "BACH1", "detection_method": null}} +{"question_id": "PPIL1-0988", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: NRIP2 (Q9BQI9)\n Function: Down-regulates transcriptional activation by nuclear receptors such as NR1F2\n Location: Nucleus\n\nProtein 2: XPO4 (Q9C0E2)\n Function: Mediates the nuclear export of proteins (cargos), such as EIF5A, SMAD3 and isoform M2 of PKM (PKM2) (PubMed:10944119, PubMed:16449645, PubMed:26787900). In the nucleus binds cooperatively to its cargo\n Location: Cytoplasm\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between NRIP2 and XPO4 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2216843, "gene_symbol_1": "NRIP2", "gene_symbol_2": "XPO4", "detection_method": null}} +{"question_id": "PPIL1-0989", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: PCDHAC1 (Q9H158)\n Function: Potential calcium-dependent cell-adhesion protein. May be involved in the establishment and maintenance of specific neuronal connections in the brain\n Location: Cell membrane\n\nProtein 2: SLIRP-OT1 (Q8N769)\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between PCDHAC1 and SLIRP-OT1 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2167670, "gene_symbol_1": "PCDHAC1", "gene_symbol_2": "SLIRP-OT1", "detection_method": null}} +{"question_id": "PPIL1-0990", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: RAC1 (P63000)\n Function: Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses\n Location: Cell membrane\n\nProtein 2: EMP2 (P54851)\n Function: Functions as a key regulator of cell membrane composition by regulating protein surface expression. Also, plays a role in regulation of processes including cell migration, cell proliferation, cell con\n Location: Golgi apparatus membrane\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between RAC1 and EMP2 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1996458, "gene_symbol_1": "RAC1", "gene_symbol_2": "EMP2", "detection_method": null}} +{"question_id": "PPIL1-0991", "task": "ppi-l1", "split": "val", "difficulty": "hard", "context_text": "Protein 1: MECOM (Q03112)\n Function: Functions as a transcriptional regulator binding to DNA sequences in the promoter region of target genes and regulating positively or negatively their expression. Oncogene which plays a role in develo\n Location: Nucleus\n\nProtein 2: RAD54L2 (Q9Y4B4)\n Function: Helicase that modulates androgen receptor (AR)-dependent transactivation in a promoter-dependent manner (By similarity). May stimulate the release of RNA polymerase II, that has paused near the transc\n Location: Nucleus\n\nEvidence: No significant association between MECOM and RAD54L2 was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2024963, "gene_symbol_1": "MECOM", "gene_symbol_2": "RAD54L2", "detection_method": null}} +{"question_id": "PPIL1-0992", "task": "ppi-l1", "split": "val", "difficulty": "hard", "context_text": "Protein 1: SECISBP2 (Q96T21)\n Function: mRNA-binding protein that binds to the SECIS (selenocysteine insertion sequence) element present in the 3'-UTR of mRNAs encoding selenoproteins and facilitates the incorporation of the rare amino acid\n Location: Nucleus\n\nProtein 2: UCHL1 (P09936)\n Function: Deubiquitinase that plays a role in the regulation of several processes such as maintenance of synaptic function, cardiac function, inflammatory response or osteoclastogenesis (PubMed:22212137, PubMed\n Location: Cytoplasm\n\nEvidence: No significant association between SECISBP2 and UCHL1 was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1890694, "gene_symbol_1": "SECISBP2", "gene_symbol_2": "UCHL1", "detection_method": null}} +{"question_id": "PPIL1-0993", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: ACSL5 (Q9ULC5)\n Function: Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoAs for both synthesis of cellular lipids, and degradation via beta-oxidation (PubMed:17681178, PubMed:22633490, PubMed:24\n Location: Mitochondrion\n\nProtein 2: GDPD1 (Q8N9F7)\n Function: Hydrolyzes lysoglycerophospholipids to produce lysophosphatidic acid (LPA) and the corresponding amines (PubMed:25596343, PubMed:27637550). Shows a preference for 1-O-alkyl-sn-glycero-3-phosphocholine\n Location: Cytoplasm\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between ACSL5 and GDPD1 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2170036, "gene_symbol_1": "ACSL5", "gene_symbol_2": "GDPD1", "detection_method": null}} +{"question_id": "PPIL1-0994", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: NCF4 (Q15080)\n Function: Subunit of the phagocyte NADPH oxidase complex that mediates the transfer of electrons from cytosolic NADPH to O2 to produce the superoxide anion (O2(-)) (Probable). In the activated complex, electron\n Location: Cytoplasm, cytosol\n\nProtein 2: ELN (P15502)\n Function: Major structural protein of tissues such as aorta and nuchal ligament, which must expand rapidly and recover completely. Molecular determinant of the late arterial morphogenesis, stabilizing arterial \n Location: Secreted, extracellular space, extracellular matrix\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between NCF4 and ELN is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1911595, "gene_symbol_1": "NCF4", "gene_symbol_2": "ELN", "detection_method": null}} +{"question_id": "PPIL1-0995", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: ETFA (P13804)\n Function: Heterodimeric electron transfer flavoprotein that accepts electrons from several mitochondrial dehydrogenases, including acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase (PubMed:10356\n Location: Mitochondrion matrix\n\nProtein 2: ADPRHL1 (Q8NDY3)\n Function: Required for myofibril assembly and outgrowth of the cardiac chambers in the developing heart (By similarity). Appears to be catalytically inactive, showing no activity against O-acetyl-ADP-ribose (By\n Location: Cytoplasm, myofibril, sarcomere\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between ETFA and ADPRHL1.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1907687, "gene_symbol_1": "ETFA", "gene_symbol_2": "ADPRHL1", "detection_method": null}} +{"question_id": "PPIL1-0996", "task": "ppi-l1", "split": "val", "difficulty": "hard", "context_text": "Protein 1: CFL1 (P23528)\n Function: Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity (PubMed:11812157). In conjunction with the subcortical maternal complex (SCMC), plays an essential role for zygotes to progre\n Location: Nucleus matrix\n\nProtein 2: TBPL2 (Q6SJ96)\n Function: Transcription factor required in complex with TAF3 for the differentiation of myoblasts into myocytes. The complex replaces TFIID at specific promoters at an early stage in the differentiation process\n Location: Cytoplasm\n\nEvidence: No significant association between CFL1 and TBPL2 was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1930881, "gene_symbol_1": "CFL1", "gene_symbol_2": "TBPL2", "detection_method": null}} +{"question_id": "PPIL1-0997", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: SULT2B1 (O00204)\n Function: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation. Responsible for the sulfation of cholesterol (PubMed:12145317, PubMed:19589\n Location: Cytoplasm, cytosol\n\nProtein 2: STK38 (Q15208)\n Function: Serine/threonine-protein kinase that acts as a negative regulator of MAP3K1/2 signaling (PubMed:12493777, PubMed:15197186, PubMed:17906693, PubMed:7761441). Converts MAP3K2 from its phosphorylated for\n Location: Nucleus\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between SULT2B1 and STK38.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1784035, "gene_symbol_1": "SULT2B1", "gene_symbol_2": "STK38", "detection_method": null}} +{"question_id": "PPIL1-0998", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: PKIA (P61925)\n Function: Extremely potent competitive inhibitor of cAMP-dependent protein kinase activity, this protein interacts with the catalytic subunit of the enzyme after the cAMP-induced dissociation of its regulatory \n\nProtein 2: VWDE (Q8N2E2)\n Location: Secreted\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between PKIA and VWDE is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2008850, "gene_symbol_1": "PKIA", "gene_symbol_2": "VWDE", "detection_method": null}} +{"question_id": "PPIL1-0999", "task": "ppi-l1", "split": "val", "difficulty": "hard", "context_text": "Protein 1: CCDC106 (Q9BWC9)\n Function: Promotes the degradation of p53/TP53 protein and inhibits its transactivity\n Location: Nucleus\n\nProtein 2: PRDX4 (Q13162)\n Function: Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by \n Location: Cytoplasm\n\nEvidence: No significant association between CCDC106 and PRDX4 was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2039556, "gene_symbol_1": "CCDC106", "gene_symbol_2": "PRDX4", "detection_method": null}} +{"question_id": "PPIL1-1000", "task": "ppi-l1", "split": "val", "difficulty": "hard", "context_text": "Protein 1: ZNF696 (Q9H7X3)\n Function: May be involved in transcriptional regulation\n Location: Nucleus\n\nProtein 2: VTI1A (Q96AJ9)\n Function: V-SNARE that mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane. These interactions are proposed to mediate aspects of the specificity of vesicle trafficking\n Location: Cytoplasmic vesicle\n\nEvidence: No significant association between ZNF696 and VTI1A was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2198893, "gene_symbol_1": "ZNF696", "gene_symbol_2": "VTI1A", "detection_method": null}} +{"question_id": "PPIL1-1001", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: SIK2 (Q9H0K1)\n Function: Serine/threonine-protein kinase that plays a role in many biological processes such as fatty acid oxidation, autophagy, immune response or glucose metabolism (PubMed:23322770, PubMed:26983400). Phosph\n Location: Cytoplasm\n\nProtein 2: GPR34 (Q9UPC5)\n Function: G-protein-coupled receptor of lysophosphatidylserine (LysoPS) that plays different roles in immune response (PubMed:16460680). Acts a damage-sensing receptor that triggers tissue repair upon recogniti\n Location: Cell membrane\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between SIK2 and GPR34 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2225309, "gene_symbol_1": "SIK2", "gene_symbol_2": "GPR34", "detection_method": null}} +{"question_id": "PPIL1-1002", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: SRBD1 (Q8N5C6)\n Function: DNA- and histone-binding protein that localizes to the central axes of mitotic chromosomes (PubMed:39955279, PubMed:40106440). Promotes chromosome segregation by facilitating TOP2A recruitment to mito\n Location: Nucleus\n\nProtein 2: TAFA3 (Q7Z5A8)\n Function: Plays a role in the regulation of microglia polarization\n Location: Secreted\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between SRBD1 and TAFA3.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2138170, "gene_symbol_1": "SRBD1", "gene_symbol_2": "TAFA3", "detection_method": null}} +{"question_id": "PPIL1-1003", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: CCDC90B (Q9GZT6)\n Location: Mitochondrion membrane\n\nProtein 2: ENDOG (Q14249)\n Function: Endonuclease that preferentially catalyzes the cleavage of double-stranded 5-hydroxymethylcytosine (5hmC)-modified DNA (PubMed:25355512). The 5hmC-modified nucleotide does not increase the binding aff\n Location: Mitochondrion\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between CCDC90B and ENDOG.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2050148, "gene_symbol_1": "CCDC90B", "gene_symbol_2": "ENDOG", "detection_method": null}} +{"question_id": "PPIL1-1004", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: MTA2 (O94776)\n Function: May function as a transcriptional coregulator (PubMed:16428440, PubMed:28977666). Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin (PubMed:\n Location: Nucleus\n\nProtein 2: SOX21 (Q9Y651)\n Function: May play a role as an activator of transcription of OPRM1. Overexpression of SOX21 can up-regulate the OPRM1 distal promoter activity in mor-expressing neuronal cells. May play a role in ameloblast di\n Location: Nucleus\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between MTA2 and SOX21 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1847632, "gene_symbol_1": "MTA2", "gene_symbol_2": "SOX21", "detection_method": null}} +{"question_id": "PPIL1-1005", "task": "ppi-l1", "split": "val", "difficulty": "hard", "context_text": "Protein 1: RNF175 (Q8N4F7)\n Location: Membrane\n\nProtein 2: COX16 (Q9P0S2)\n Function: Required for the assembly of the mitochondrial respiratory chain complex IV (CIV), also known as cytochrome c oxidase (PubMed:29355485, PubMed:29381136, PubMed:33169484). Promotes the insertion of cop\n Location: Mitochondrion inner membrane\n\nEvidence: No significant association between RNF175 and COX16 was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2164371, "gene_symbol_1": "RNF175", "gene_symbol_2": "COX16", "detection_method": null}} +{"question_id": "PPIL1-1006", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: WAS (P42768)\n Function: Effector protein for Rho-type GTPases that regulates actin filament reorganization via its interaction with the Arp2/3 complex (PubMed:12235133, PubMed:12769847, PubMed:16275905). Important for effici\n Location: Cytoplasm, cytoskeleton\n\nProtein 2: CERKL (Q49MI3)\n Function: Has no detectable ceramide-kinase activity. Overexpression of CERKL protects cells from apoptosis in oxidative stress conditions\n Location: Cytoplasm\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between WAS and CERKL.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1971246, "gene_symbol_1": "WAS", "gene_symbol_2": "CERKL", "detection_method": null}} +{"question_id": "PPIL1-1007", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: PPP1R11 (O60927)\n Function: Atypical E3 ubiquitin-protein ligase which ubiquitinates TLR2 at 'Lys-754' leading to its degradation by the proteasome. Plays a role in regulating inflammatory cytokine release and gram-positive bact\n\nProtein 2: UQCRFS1 (P47985)\n Function: Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation (PubMed:3\n Location: Mitochondrion inner membrane\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between PPP1R11 and UQCRFS1 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1829642, "gene_symbol_1": "PPP1R11", "gene_symbol_2": "UQCRFS1", "detection_method": null}} +{"question_id": "PPIL1-1008", "task": "ppi-l1", "split": "val", "difficulty": "hard", "context_text": "Protein 1: HS2ST1 (K7EP71)\n Function: Catalyzes the transfer of a sulfo group from 3'-phospho-5'-adenylyl sulfate (PAPS) to the 2-OH position of iduronic acid (IdoA) or glucuronic acid (GlcA) within the heparan sulfate (HS) chain and part\n Location: Golgi apparatus membrane\n\nProtein 2: SAMD14 (Q8IZD0)\n\nEvidence: No significant association between HS2ST1 and SAMD14 was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1780656, "gene_symbol_1": "HS2ST1", "gene_symbol_2": "SAMD14", "detection_method": null}} +{"question_id": "PPIL1-1009", "task": "ppi-l1", "split": "val", "difficulty": "hard", "context_text": "Protein 1: LRP5L (A4QPB2)\n\nProtein 2: SH3TC1 (Q8TE82)\n\nEvidence: No significant association between LRP5L and SH3TC1 was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1742703, "gene_symbol_1": "LRP5L", "gene_symbol_2": "SH3TC1", "detection_method": null}} +{"question_id": "PPIL1-1010", "task": "ppi-l1", "split": "val", "difficulty": "hard", "context_text": "Protein 1: NECAB3 (Q96P71)\n Function: Inhibits the interaction of APBA2 with amyloid-beta precursor protein (APP), and hence allows formation of amyloid-beta. May enhance the activity of HIF1A and thus promote glycolysis under normoxic co\n Location: Golgi apparatus\n\nProtein 2: PAPPA2 (Q9BXP8)\n Function: Metalloproteinase which specifically cleaves insulin-like growth factor binding protein (IGFBP)-5 at the '163-Ser-|-Lys-164' bond. Shows limited proteolysis toward IGFBP-3\n Location: Secreted\n\nEvidence: No significant association between NECAB3 and PAPPA2 was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2210672, "gene_symbol_1": "NECAB3", "gene_symbol_2": "PAPPA2", "detection_method": null}} +{"question_id": "PPIL1-1011", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: CEP78 (Q5JTW2)\n Function: Centriole wall protein that localizes to mature centrioles and regulates centriole and cilia biogenesis (PubMed:27246242, PubMed:27588451, PubMed:28242748, PubMed:34259627). Involved in centrosome dup\n Location: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome\n\nProtein 2: FAM174C (Q9BVV8)\n Location: Membrane\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between CEP78 and FAM174C.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2088986, "gene_symbol_1": "CEP78", "gene_symbol_2": "FAM174C", "detection_method": null}} +{"question_id": "PPIL1-1012", "task": "ppi-l1", "split": "val", "difficulty": "medium", "context_text": "Protein 1: CCT2 (P78371)\n Function: Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of actin, tubulin and other proteins upon ATP hydrolysis (PubMed:25467444, PubMed:364937\n Location: Cytoplasm\n\nProtein 2: PPP2R1B (P30154)\n Function: The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between CCT2 and PPP2R1B.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1942065, "gene_symbol_1": "CCT2", "gene_symbol_2": "PPP2R1B", "detection_method": null}} +{"question_id": "PPIL1-1013", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: EXOC4 (Q96A65)\n Function: Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane\n Location: Midbody, Midbody ring\n\nProtein 2: SECISBP2 (Q96T21)\n Function: mRNA-binding protein that binds to the SECIS (selenocysteine insertion sequence) element present in the 3'-UTR of mRNAs encoding selenoproteins and facilitates the incorporation of the rare amino acid\n Location: Nucleus\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between EXOC4 and SECISBP2 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2198542, "gene_symbol_1": "EXOC4", "gene_symbol_2": "SECISBP2", "detection_method": null}} +{"question_id": "PPIL1-1014", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: ZNF562 (Q6V9R5)\n Function: May be involved in transcriptional regulation\n Location: Nucleus\n\nProtein 2: DYTN (A2CJ06)\n Location: Cell membrane\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between ZNF562 and DYTN is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1739633, "gene_symbol_1": "ZNF562", "gene_symbol_2": "DYTN", "detection_method": null}} +{"question_id": "PPIL1-1015", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: P2RY6 (Q15077)\n Function: Receptor for extracellular UDP > UTP > ATP. The activity of this receptor is mediated by G proteins which activate a phosphatidylinositol-calcium second messenger system\n Location: Cell membrane\n\nProtein 2: KLF2 (Q9Y5W3)\n Function: Transcription factor that binds to the CACCC box in the promoter of target genes such as HBB/beta globin or NOV and activates their transcription (PubMed:21063504). Might be involved in transcriptiona\n Location: Nucleus\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between P2RY6 and KLF2 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2057745, "gene_symbol_1": "P2RY6", "gene_symbol_2": "KLF2", "detection_method": null}} +{"question_id": "PPIL1-1016", "task": "ppi-l1", "split": "val", "difficulty": "easy", "context_text": "Protein 1: TM9SF3 (Q9HD45)\n Location: Membrane\n\nProtein 2: C4orf36 (Q96KX1)\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between TM9SF3 and C4orf36 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2207632, "gene_symbol_1": "TM9SF3", "gene_symbol_2": "C4orf36", "detection_method": null}} +{"question_id": "PPIL1-1017", "task": "ppi-l1", "split": "val", "difficulty": "hard", "context_text": "Protein 1: ITLN2 (Q8WWU7)\n Function: May play a role in the defense system against pathogens\n Location: Secreted\n\nProtein 2: LIPT2 (A6NK58)\n Function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein (octanoyl-ACP) onto the lipoyl domains of lipoate-dependent enzymes such as the protein H of the glycin\n Location: Mitochondrion\n\nEvidence: No significant association between ITLN2 and LIPT2 was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1753035, "gene_symbol_1": "ITLN2", "gene_symbol_2": "LIPT2", "detection_method": null}} +{"question_id": "PPIL1-1018", "task": "ppi-l1", "split": "val", "difficulty": "hard", "context_text": "Protein 1: AQP2 (P41181)\n Function: Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient (Pu\n Location: Apical cell membrane\n\nProtein 2: SLC11A2 (P49281)\n Function: Proton-coupled metal ion symporter operating with a proton to metal ion stoichiometry of 1:1 (PubMed:17109629, PubMed:17293870, PubMed:22736759, PubMed:25326704, PubMed:25491917). Selectively transpor\n Location: Early endosome membrane\n\nEvidence: No significant association between AQP2 and SLC11A2 was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1968164, "gene_symbol_1": "AQP2", "gene_symbol_2": "SLC11A2", "detection_method": null}} +{"question_id": "PPIL1-1019", "task": "ppi-l1", "split": "val", "difficulty": "hard", "context_text": "Protein 1: GLRX (P35754)\n Function: Has a glutathione-disulfide oxidoreductase activity in the presence of NADPH and glutathione reductase. Reduces low molecular weight disulfides and proteins\n Location: Cytoplasm\n\nProtein 2: ACSS3 (Q9H6R3)\n Function: Catalyzes the synthesis of acetyl-CoA from short-chain fatty acids (PubMed:28003429). Propionate is the preferred substrate (PubMed:28003429). Can utilize acetate and butyrate with a much lower affini\n Location: Mitochondrion matrix\n\nEvidence: No significant association between GLRX and ACSS3 was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1962907, "gene_symbol_1": "GLRX", "gene_symbol_2": "ACSS3", "detection_method": null}} +{"question_id": "PPIL1-1020", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: OPN3 (Q9H1Y3)\n Function: G protein-coupled receptor which selectively activates G(i/o) proteins via ultraviolet A (UVA) light-mediated activation in the skin, thereby decreasing cellular cAMP (PubMed:28842328, PubMed:31380578\n Location: Cell membrane\n\nProtein 2: NLRP2 (Q9NX02)\n Function: Suppresses TNF- and CD40-induced NFKB1 activity at the level of the IKK complex, by inhibiting NFKBIA degradation induced by TNF. When associated with PYCARD, activates CASP1, leading to the secretion\n Location: Cytoplasm\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between OPN3 and NLRP2 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2226136, "gene_symbol_1": "OPN3", "gene_symbol_2": "NLRP2", "detection_method": null}} +{"question_id": "PPIL1-1021", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: GARIN4 (Q8IYT1)\n Function: RAB2B effector protein required for the compacted Golgi morphology, probably through interaction with small GTPase RAB2B\n Location: Golgi apparatus\n\nProtein 2: RMDN1 (Q96DB5)\n Location: Cytoplasm\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between GARIN4 and RMDN1.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2157597, "gene_symbol_1": "GARIN4", "gene_symbol_2": "RMDN1", "detection_method": null}} +{"question_id": "PPIL1-1022", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: ZNF19 (P17023)\n Function: May be involved in transcriptional regulation\n Location: Nucleus\n\nProtein 2: TSPAN6 (O43657)\n Location: Membrane\n\nEvidence: No significant association between ZNF19 and TSPAN6 was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1815717, "gene_symbol_1": "ZNF19", "gene_symbol_2": "TSPAN6", "detection_method": null}} +{"question_id": "PPIL1-1023", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: MYL1 (P05976)\n Function: Non-regulatory myosin light chain required for proper formation and/or maintenance of myofibers, and thus appropriate muscle function\n\nProtein 2: ZNF471 (Q9BX82)\n Function: May be involved in transcriptional regulation\n Location: Nucleus\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between MYL1 and ZNF471 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1880227, "gene_symbol_1": "MYL1", "gene_symbol_2": "ZNF471", "detection_method": null}} +{"question_id": "PPIL1-1024", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: EPO (P01588)\n Function: Hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass (PubMed:28283061). Binds to EPOR leadin\n Location: Secreted\n\nProtein 2: AVPR1B (P47901)\n Function: Receptor for arginine vasopressin. The activity of this receptor is mediated by G proteins which activate a phosphatidyl-inositol-calcium second messenger system\n Location: Cell membrane\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between EPO and AVPR1B.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1871076, "gene_symbol_1": "EPO", "gene_symbol_2": "AVPR1B", "detection_method": null}} +{"question_id": "PPIL1-1025", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: IMP4 (Q96G21)\n Function: Component of the 60-80S U3 small nucleolar ribonucleoprotein (U3 snoRNP). Required for the early cleavages during pre-18S ribosomal RNA processing (PubMed:12655004). Part of the small subunit (SSU) pr\n Location: Nucleus, nucleolus\n\nProtein 2: ZNF839 (A8K0R7)\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between IMP4 and ZNF839 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1757924, "gene_symbol_1": "IMP4", "gene_symbol_2": "ZNF839", "detection_method": null}} +{"question_id": "PPIL1-1026", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: RRP12 (Q5JTH9)\n Location: Nucleus, nucleolus\n\nProtein 2: DPP10 (Q8N608)\n Function: Promotes cell surface expression of the potassium channel KCND2 (PubMed:15454437). Modulates the activity and gating characteristics of the potassium channel KCND2 (PubMed:15454437). Has no dipeptidyl\n Location: Cell membrane\n\nEvidence: No significant association between RRP12 and DPP10 was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2088875, "gene_symbol_1": "RRP12", "gene_symbol_2": "DPP10", "detection_method": null}} +{"question_id": "PPIL1-1027", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: TMED9 (Q9BVK6)\n Function: Appears to be involved in vesicular protein trafficking, mainly in the early secretory pathway. In COPI vesicle-mediated retrograde transport involved in the coatomer recruitment to membranes of the e\n Location: Endoplasmic reticulum membrane\n\nProtein 2: ZNF586 (Q9NXT0)\n Function: May be involved in transcriptional regulation\n Location: Nucleus\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between TMED9 and ZNF586 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2219923, "gene_symbol_1": "TMED9", "gene_symbol_2": "ZNF586", "detection_method": null}} +{"question_id": "PPIL1-1028", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: MYO1D (O94832)\n Function: Unconventional myosin that functions as actin-based motor protein with ATPase activity (By similarity). Plays a role in endosomal protein trafficking, and especially in the transfer of cargo proteins \n Location: Cytoplasm\n\nProtein 2: MYH10 (P35580)\n Function: Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Involved with LARP6 in the stabilization of type I collagen mRNAs for C\n Location: Cell projection, lamellipodium\n\nEvidence: No significant association between MYO1D and MYH10 was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1848689, "gene_symbol_1": "MYO1D", "gene_symbol_2": "MYH10", "detection_method": null}} +{"question_id": "PPIL1-1029", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: SEMA3G (Q9NS98)\n Function: Has chemorepulsive activities for sympathetic axons. Ligand of NRP2 (By similarity)\n Location: Secreted\n\nProtein 2: E2F5 (Q15329)\n Function: Transcriptional activator that binds to E2F sites, these sites are present in the promoter of many genes whose products are involved in cell proliferation. May mediate growth factor-initiated signal t\n Location: Nucleus\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between SEMA3G and E2F5.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2059314, "gene_symbol_1": "SEMA3G", "gene_symbol_2": "E2F5", "detection_method": null}} +{"question_id": "PPIL1-1030", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: OAZ2 (O95190)\n Function: Ornithine decarboxylase (ODC) antizyme protein that negatively regulates ODC activity and intracellular polyamine biosynthesis and uptake in response to increased intracellular polyamine levels. Binds\n Location: Nucleus\n\nProtein 2: ACADSB (P45954)\n Function: Short and branched chain specific acyl-CoA dehydrogenase that catalyzes the proR-proR stereospecific alpha,beta-dehydrogenation of fatty acyl-CoA thioesters using the electron transfer flavoprotein (E\n Location: Mitochondrion matrix\n\nEvidence: No significant association between OAZ2 and ACADSB was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1853614, "gene_symbol_1": "OAZ2", "gene_symbol_2": "ACADSB", "detection_method": null}} +{"question_id": "PPIL1-1031", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: TTC36 (A6NLP5)\n\nProtein 2: SMAP (O00193)\n\nEvidence: No significant association between TTC36 and SMAP was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1754306, "gene_symbol_1": "TTC36", "gene_symbol_2": "SMAP", "detection_method": null}} +{"question_id": "PPIL1-1032", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: POU4F1 (Q01851)\n Function: Multifunctional transcription factor with different regions mediating its different effects. Acts by binding (via its C-terminal domain) to sequences related to the consensus octamer motif 5'-ATGCAAAT\n Location: Nucleus\n\nProtein 2: MICAL3 (Q7RTP6)\n Function: Monooxygenase that promotes depolymerization of F-actin by mediating oxidation of specific methionine residues on actin to form methionine-sulfoxide, resulting in actin filament disassembly and preven\n Location: Cytoplasm\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between POU4F1 and MICAL3.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2022215, "gene_symbol_1": "POU4F1", "gene_symbol_2": "MICAL3", "detection_method": null}} +{"question_id": "PPIL1-1033", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: MS4A3 (Q96HJ5)\n Function: Hematopoietic modulator for the G1-S cell cycle transition. Modulates the level of phosphorylation of cyclin-dependent kinase 2 (CDK2) through its direct binding to cyclin-dependent kinase inhibitor 3\n Location: Endomembrane system\n\nProtein 2: NDUFS4 (O43181)\n Function: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons fro\n Location: Mitochondrion inner membrane\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between MS4A3 and NDUFS4.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1809523, "gene_symbol_1": "MS4A3", "gene_symbol_2": "NDUFS4", "detection_method": null}} +{"question_id": "PPIL1-1034", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: PLCG1 (P19174)\n Function: Mediates the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). Plays an important role in the regulation of intracellular signaling cascades. Be\n Location: Cell projection, lamellipodium\n\nProtein 2: KIFC1 (Q9BW19)\n Function: Minus end-directed microtubule-dependent motor required for bipolar spindle formation (PubMed:15843429). May contribute to movement of early endocytic vesicles (By similarity). Regulates cilium format\n Location: Nucleus\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between PLCG1 and KIFC1 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1920096, "gene_symbol_1": "PLCG1", "gene_symbol_2": "KIFC1", "detection_method": null}} +{"question_id": "PPIL1-1035", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: S100A6 (P06703)\n Function: May function as calcium sensor and modulator, contributing to cellular calcium signaling. May function by interacting with other proteins, such as TPR-containing proteins, and indirectly play a role i\n Location: Nucleus envelope\n\nProtein 2: TRIM28 (Q13263)\n Function: Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, an\n Location: Nucleus\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between S100A6 and TRIM28.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1881214, "gene_symbol_1": "S100A6", "gene_symbol_2": "TRIM28", "detection_method": null}} +{"question_id": "PPIL1-1036", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: PARP3 (Q9Y6F1)\n Function: Mono-ADP-ribosyltransferase that mediates mono-ADP-ribosylation of target proteins and plays a key role in the response to DNA damage (PubMed:16924674, PubMed:19354255, PubMed:20064938, PubMed:2121172\n Location: Nucleus\n\nProtein 2: ACCSL (Q4AC99)\n\nEvidence: No significant association between PARP3 and ACCSL was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2079469, "gene_symbol_1": "PARP3", "gene_symbol_2": "ACCSL", "detection_method": null}} +{"question_id": "PPIL1-1037", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: CAPZA3 (Q96KX2)\n Function: F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping prot\n Location: Cytoplasm, cytoskeleton\n\nProtein 2: SUGT1 (Q9Y2Z0)\n Function: May play a role in ubiquitination and subsequent proteasomal degradation of target proteins\n Location: Cytoplasm\n\nEvidence: No significant association between CAPZA3 and SUGT1 was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2207655, "gene_symbol_1": "CAPZA3", "gene_symbol_2": "SUGT1", "detection_method": null}} +{"question_id": "PPIL1-1038", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: ZNF35 (P13682)\n Function: May be involved in transcriptional regulation. Involved in cell differentiation and/or proliferation\n Location: Nucleus\n\nProtein 2: PRDM2 (Q13029)\n Function: S-adenosyl-L-methionine-dependent histone methyltransferase that specifically methylates 'Lys-9' of histone H3. May function as a DNA-binding transcription factor. Binds to the macrophage-specific TPA\n Location: Nucleus\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between ZNF35 and PRDM2.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1907169, "gene_symbol_1": "ZNF35", "gene_symbol_2": "PRDM2", "detection_method": null}} +{"question_id": "PPIL1-1039", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: AKR7A2 (O43488)\n Function: Catalyzes the NADPH-dependent reduction of succinic semialdehyde to gamma-hydroxybutyrate. May have an important role in producing the neuromodulator gamma-hydroxybutyrate (GHB). Has broad substrate s\n Location: Mitochondrion\n\nProtein 2: TUSC3 (Q13454)\n Function: Acts as accessory component of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine \n Location: Endoplasmic reticulum membrane\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between AKR7A2 and TUSC3 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1813487, "gene_symbol_1": "AKR7A2", "gene_symbol_2": "TUSC3", "detection_method": null}} +{"question_id": "PPIL1-1040", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: TLE4 (Q04727)\n Function: Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by PAX5, and by CTNNB1 and TCF family members in Wnt signaling. The effect\n Location: Nucleus\n\nProtein 2: IPPK (Q9H8X2)\n Function: Phosphorylates Ins(1,3,4,5,6)P5 at position 2 to form Ins(1,2,3,4,5,6)P6 (InsP6 or phytate). InsP6 is involved in many processes such as mRNA export, non-homologous end-joining, endocytosis, ion chann\n Location: Cytoplasm\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between TLE4 and IPPK is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2026481, "gene_symbol_1": "TLE4", "gene_symbol_2": "IPPK", "detection_method": null}} +{"question_id": "PPIL1-1041", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: RIC8A (Q9NPQ8)\n Function: Chaperone that specifically binds and folds nascent G alpha proteins prior to G protein heterotrimer formation, promoting their stability and activity: folds GNAI1, GNAO1, GNA13 and GNAQ (By similarit\n Location: Cytoplasm, cell cortex\n\nProtein 2: CCL8 (P80075)\n Function: Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. May play a role in neoplasia and inflammatory host responses. This protein can bind heparin. The processed form MCP-\n Location: Secreted\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between RIC8A and CCL8.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2015707, "gene_symbol_1": "RIC8A", "gene_symbol_2": "CCL8", "detection_method": null}} +{"question_id": "PPIL1-1042", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: TM9SF4 (Q92544)\n Function: Associates with proteins harboring glycine-rich transmembrane domains and ensures their efficient localization to the cell surface (PubMed:25999474). Regulates the assembly and activity of V-ATPase in\n Location: Membrane\n\nProtein 2: KLK13 (Q9UKR3)\n Location: Secreted\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between TM9SF4 and KLK13.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2193570, "gene_symbol_1": "TM9SF4", "gene_symbol_2": "KLK13", "detection_method": null}} +{"question_id": "PPIL1-1043", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: MDM4 (O15151)\n Function: Contributes to p53/TP53 regulation (PubMed:32300648). Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding their transcriptional activation domains. Inhibits degradation\n Location: Nucleus\n\nProtein 2: C3 (P01024)\n Function: Precursor of non-enzymatic components of the classical, alternative, lectin and GZMK complement pathways, which consist in a cascade of proteins that leads to phagocytosis and breakdown of pathogens a\n Location: Secreted\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between MDM4 and C3 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1802183, "gene_symbol_1": "MDM4", "gene_symbol_2": "C3", "detection_method": null}} +{"question_id": "PPIL1-1044", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: PHF5A (Q7RTV0)\n Function: Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs (PubMed:12234937, PubMed:27720643, PubMed:28541300, PubMed:3\n Location: Nucleus\n\nProtein 2: JMJD8 (Q96S16)\n Function: Functions as a positive regulator of TNF-induced NF-kappa-B signaling (PubMed:27671354). Regulates angiogenesis and cellular metabolism through interaction with PKM (PubMed:27199445)\n Location: Endoplasmic reticulum lumen\n\nEvidence: No significant association between PHF5A and JMJD8 was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2135083, "gene_symbol_1": "PHF5A", "gene_symbol_2": "JMJD8", "detection_method": null}} +{"question_id": "PPIL1-1045", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: SLC30A4 (O14863)\n Function: Probable proton-coupled zinc ion antiporter mediating zinc import from cytoplasm potentially into the endocytic compartment (PubMed:19521526). Controls zinc deposition in milk (By similarity)\n Location: Endosome membrane\n\nProtein 2: PDF (Q9HBH1)\n Function: Removes the formyl group from the N-terminal Met of newly synthesized proteins\n Location: Mitochondrion\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between SLC30A4 and PDF is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1797278, "gene_symbol_1": "SLC30A4", "gene_symbol_2": "PDF", "detection_method": null}} +{"question_id": "PPIL1-1046", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: LMNA (P02545)\n Function: Lamins are intermediate filament proteins that assemble into a filamentous meshwork, and which constitute the major components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the i\n Location: Nucleus lamina\n\nProtein 2: LYPD4 (Q6UWN0)\n Location: Cell membrane\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between LMNA and LYPD4.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1871976, "gene_symbol_1": "LMNA", "gene_symbol_2": "LYPD4", "detection_method": null}} +{"question_id": "PPIL1-1047", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: TGM2 (P21980)\n Function: Calcium-dependent acyltransferase that catalyzes the formation of covalent bonds between peptide-bound glutamine and various primary amines, such as gamma-amino group of peptide-bound lysine, or mono-\n Location: Cytoplasm, cytosol\n\nProtein 2: LIN9 (Q5TKA1)\n Function: Acts as a tumor suppressor. Inhibits DNA synthesis. Its ability to inhibit oncogenic transformation is mediated through its association with RB1. Plays a role in the expression of genes required for t\n Location: Nucleus, nucleoplasm\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between TGM2 and LIN9 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1926598, "gene_symbol_1": "TGM2", "gene_symbol_2": "LIN9", "detection_method": null}} +{"question_id": "PPIL1-1048", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: TMEM218 (A2RU14)\n Function: May be involved in ciliary biogenesis or function\n Location: Membrane\n\nProtein 2: TOM1 (O60784)\n Function: Adapter protein that plays a role in the intracellular membrane trafficking of ubiquitinated proteins, thereby participating in autophagy, ubiquitination-dependent signaling and receptor recycling pat\n Location: Cytoplasm\n\nEvidence: No significant association between TMEM218 and TOM1 was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1740146, "gene_symbol_1": "TMEM218", "gene_symbol_2": "TOM1", "detection_method": null}} +{"question_id": "PPIL1-1049", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: RAMP1 (O60894)\n Function: Accessory protein that interacts with and modulates the function of G-protein coupled receptors including calcitonin gene-related peptide type 1 receptor (CALCRL) and calcitonin receptor (CALCR) (PubM\n Location: Cell membrane\n\nProtein 2: FAR1 (Q8WVX9)\n Function: Catalyzes the reduction of saturated and unsaturated C16 or C18 fatty acyl-CoA to fatty alcohols (PubMed:15220348, PubMed:24108123, PubMed:35238077). It plays an essential role in the production of et\n Location: Peroxisome membrane\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between RAMP1 and FAR1 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1829224, "gene_symbol_1": "RAMP1", "gene_symbol_2": "FAR1", "detection_method": null}} +{"question_id": "PPIL1-1050", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: OGFR (Q9NZT2)\n Function: Receptor for opioid growth factor (OGF), also known as Met-enkephalin. Seems to be involved in growth regulation\n Location: Cytoplasm\n\nProtein 2: FGF3 (P11487)\n Function: Plays an important role in the regulation of embryonic development, cell proliferation, and cell differentiation. Required for normal ear development\n Location: Secreted\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between OGFR and FGF3.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1902507, "gene_symbol_1": "OGFR", "gene_symbol_2": "FGF3", "detection_method": null}} +{"question_id": "PPIL1-1051", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: TNFRSF10B (O14763)\n Function: Receptor for the cytotoxic ligand TNFSF10/TRAIL (PubMed:10549288). The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) perform\n Location: Membrane\n\nProtein 2: BRD9 (Q9H8M2)\n Function: Plays a role in chromatin remodeling and regulation of transcription (PubMed:22464331, PubMed:26365797). Acts as a chromatin reader that recognizes and binds acylated histones: binds histones that are\n Location: Nucleus\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between TNFRSF10B and BRD9.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1795377, "gene_symbol_1": "TNFRSF10B", "gene_symbol_2": "BRD9", "detection_method": null}} +{"question_id": "PPIL1-1052", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: TUBGCP6 (Q96RT7)\n Function: Component of the gamma-tubulin ring complex (gTuRC) which mediates microtubule nucleation (PubMed:11694571, PubMed:38305685, PubMed:38609661, PubMed:39321809). The gTuRC regulates the minus-end nuclea\n Location: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome\n\nProtein 2: TEKT2 (Q9UIF3)\n Function: Microtubule inner protein (MIP) part of the dynein-decorated doublet microtubules (DMTs) in cilia and flagellar axoneme (PubMed:36191189). Plays a key role in the assembly or attachment of the inner d\n Location: Cytoplasm, cytoskeleton, cilium axoneme\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between TUBGCP6 and TEKT2 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2212639, "gene_symbol_1": "TUBGCP6", "gene_symbol_2": "TEKT2", "detection_method": null}} +{"question_id": "PPIL1-1053", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: SLC30A6 (Q6NXT4)\n Function: Has probably no intrinsic transporter activity but together with SLC30A5 forms a functional zinc ion:proton antiporter heterodimer, mediating zinc entry into the lumen of organelles along the secretor\n Location: Golgi apparatus, trans-Golgi network membrane\n\nProtein 2: KCTD6 (Q8NC69)\n Function: Probable substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex mediating the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes the ub\n Location: Cytoplasm, myofibril, sarcomere, M line\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between SLC30A6 and KCTD6 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2113270, "gene_symbol_1": "SLC30A6", "gene_symbol_2": "KCTD6", "detection_method": null}} +{"question_id": "PPIL1-1054", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: INAFM1 (C9JVW0)\n Location: Membrane\n\nProtein 2: OPA1 (O60313)\n Function: Dynamin-related GTPase that is essential for normal mitochondrial morphology by mediating fusion of the mitochondrial inner membranes, regulating cristae morphology and maintaining respiratory chain f\n Location: Mitochondrion inner membrane\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between INAFM1 and OPA1.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1768334, "gene_symbol_1": "INAFM1", "gene_symbol_2": "OPA1", "detection_method": null}} +{"question_id": "PPIL1-1055", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: CIMIP1 (Q9H1P6)\n Location: Cell projection, cilium\n\nProtein 2: DPAGT1 (Q9H3H5)\n Function: UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in prote\n Location: Endoplasmic reticulum membrane\n\nEvidence: No significant association between CIMIP1 and DPAGT1 was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2226042, "gene_symbol_1": "CIMIP1", "gene_symbol_2": "DPAGT1", "detection_method": null}} +{"question_id": "PPIL1-1056", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: DGKE (P52429)\n Function: Membrane-bound diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids (PubMed:15544348, PubMed\n Location: Membrane\n\nProtein 2: ARFGEF3 (Q5TH69)\n Function: Participates in the regulation of systemic glucose homeostasis, where it negatively regulates insulin granule biogenesis in pancreatic islet beta cells (By similarity). Also regulates glucagon granule\n Location: Cytoplasm\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between DGKE and ARFGEF3.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1991252, "gene_symbol_1": "DGKE", "gene_symbol_2": "ARFGEF3", "detection_method": null}} +{"question_id": "PPIL1-1057", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: NME2 (P22392)\n Function: Catalyzes the transfer of a gamma-phosphoryl group from a nucleoside triphosphate, mainly ATP, to a nucleoside diphosphate via a ping-pong mechanism involving a phosphohistidine intermediate, therefor\n Location: Cytoplasm\n\nProtein 2: SPATA31A6 (Q5VVP1)\n Function: May play a role in spermatogenesis\n Location: Membrane\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between NME2 and SPATA31A6 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1927643, "gene_symbol_1": "NME2", "gene_symbol_2": "SPATA31A6", "detection_method": null}} +{"question_id": "PPIL1-1058", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: ZFP91 (Q96JP5)\n Function: DNA-binding transcription factor (PubMed:34936696, PubMed:40608935). Also acts as an atypical E3 ubiquitin-protein ligase that mediates 'Lys-63'-linked ubiquitination of MAP3K14/NIK, leading to stabil\n Location: Nucleus\n\nProtein 2: HIVEP3 (Q5T1R4)\n Function: Plays a role of transcription factor; binds to recognition signal sequences (Rss heptamer) for somatic recombination of immunoglobulin and T-cell receptor gene segments; Also binds to the kappa-B moti\n Location: Cytoplasm\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between ZFP91 and HIVEP3.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2093769, "gene_symbol_1": "ZFP91", "gene_symbol_2": "HIVEP3", "detection_method": null}} +{"question_id": "PPIL1-1059", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: SAMD9L (Q8IVG5)\n Function: May be involved in endosome fusion. Mediates down-regulation of growth factor signaling via internalization of growth factor receptors\n Location: Early endosome\n\nProtein 2: ASTN2 (O75129)\n Function: Mediates recycling of the neuronal cell adhesion molecule ASTN1 to the anterior pole of the cell membrane in migrating neurons. Promotes ASTN1 internalization and intracellular transport of endocytose\n Location: Membrane\n\nEvidence: No significant association between SAMD9L and ASTN2 was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1832147, "gene_symbol_1": "SAMD9L", "gene_symbol_2": "ASTN2", "detection_method": null}} +{"question_id": "PPIL1-1060", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: VPS41 (P49754)\n Function: Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Acts as a component of the HOPS endosomal tethering c\n Location: Endosome membrane\n\nProtein 2: U2SURP (O15042)\n Location: Nucleus\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between VPS41 and U2SURP is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1800185, "gene_symbol_1": "VPS41", "gene_symbol_2": "U2SURP", "detection_method": null}} +{"question_id": "PPIL1-1061", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: MORN2 (Q502X0)\n Function: Might have a role in spermatogenesis\n Location: Cytoplasmic vesicle, secretory vesicle, acrosome\n\nProtein 2: WFDC9 (Q8NEX5)\n Location: Secreted\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between MORN2 and WFDC9 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2081711, "gene_symbol_1": "MORN2", "gene_symbol_2": "WFDC9", "detection_method": null}} +{"question_id": "PPIL1-1062", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: FGD1 (P98174)\n Function: Activates CDC42, a member of the Ras-like family of Rho- and Rac proteins, by exchanging bound GDP for free GTP. Plays a role in regulating the actin cytoskeleton and cell shape\n Location: Cytoplasm\n\nProtein 2: REG1B (P48304)\n Function: Might act as an inhibitor of spontaneous calcium carbonate precipitation. May be associated with neuronal sprouting in brain, and with brain and pancreas regeneration\n Location: Secreted\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between FGD1 and REG1B.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1978355, "gene_symbol_1": "FGD1", "gene_symbol_2": "REG1B", "detection_method": null}} +{"question_id": "PPIL1-1063", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: FBP1 (P09467)\n Function: Catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate in the presence of divalent cations, acting as a rate-limiting enzyme in gluconeogenesis. Plays a role in regulating gluco\n\nProtein 2: B4E1Z4 (B4E1Z4)\n Function: Catalytic component of the complement C3 and C5 convertase complexes. Following complement activation, recruited to the surface of pathogens by complement C4b opsonin to form the C3 convertase, or C3b\n Location: Cell surface\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between FBP1 and None is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1764586, "gene_symbol_1": "FBP1", "gene_symbol_2": null, "detection_method": null}} +{"question_id": "PPIL1-1064", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: MYL2 (P10916)\n Function: Contractile protein that plays a role in heart development and function (PubMed:23365102, PubMed:32453731). Following phosphorylation, plays a role in cross-bridge cycling kinetics and cardiac muscle \n Location: Cytoplasm, myofibril, sarcomere, A band\n\nProtein 2: SPATA31G1 (Q5VYM1)\n Function: Dispensable for normal development and fertility\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between MYL2 and SPATA31G1.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1900738, "gene_symbol_1": "MYL2", "gene_symbol_2": "SPATA31G1", "detection_method": null}} +{"question_id": "PPIL1-1065", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: EMILIN2 (Q9BXX0)\n Function: May be responsible for anchoring smooth muscle cells to elastic fibers, and may be involved not only in the formation of the elastic fiber, but also in the processes that regulate vessel assembly. Has\n Location: Secreted, extracellular space, extracellular matrix\n\nProtein 2: E2F5 (Q15329)\n Function: Transcriptional activator that binds to E2F sites, these sites are present in the promoter of many genes whose products are involved in cell proliferation. May mediate growth factor-initiated signal t\n Location: Nucleus\n\nEvidence: No significant association between EMILIN2 and E2F5 was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2059309, "gene_symbol_1": "EMILIN2", "gene_symbol_2": "E2F5", "detection_method": null}} +{"question_id": "PPIL1-1066", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: ATP6V1A (P38606)\n Function: Catalytic subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translo\n Location: Cytoplasm\n\nProtein 2: H2AZ1 (P0C0S5)\n Function: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DN\n Location: Nucleus\n\nEvidence: No significant association between ATP6V1A and H2AZ1 was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1891117, "gene_symbol_1": "ATP6V1A", "gene_symbol_2": "H2AZ1", "detection_method": null}} +{"question_id": "PPIL1-1067", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: PHKA1 (P46020)\n Function: Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. The alpha chain may bind calmodulin\n Location: Cell membrane\n\nProtein 2: ARPC5 (O15511)\n Function: Component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF) (PubMed:9230079). The Arp2/3 complex mediates the forma\n Location: Cytoplasm, cytoskeleton\n\nEvidence: No significant association between PHKA1 and ARPC5 was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1807555, "gene_symbol_1": "PHKA1", "gene_symbol_2": "ARPC5", "detection_method": null}} +{"question_id": "PPIL1-1068", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: LRRC37A3 (O60309)\n Location: Membrane\n\nProtein 2: CAPSL (Q8WWF8)\n Location: Cytoplasm\n\nEvidence: No significant association between LRRC37A3 and CAPSL was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1822353, "gene_symbol_1": "LRRC37A3", "gene_symbol_2": "CAPSL", "detection_method": null}} +{"question_id": "PPIL1-1069", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: MDFI (Q99750)\n Function: Inhibits the transactivation activity of the Myod family of myogenic factors and represses myogenesis (By similarity). Acts by associating with Myod family members and retaining them in the cytoplasm \n Location: Nucleus\n\nProtein 2: SOX9 (P48436)\n Function: Transcription factor that plays a key role in chondrocytes differentiation and skeletal development (PubMed:24038782). Specifically binds the 5'-ACAAAG-3' DNA motif present in enhancers and super-enha\n Location: Nucleus\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between MDFI and SOX9.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1978710, "gene_symbol_1": "MDFI", "gene_symbol_2": "SOX9", "detection_method": null}} +{"question_id": "PPIL1-1070", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: ATP5F1B (P06576)\n Function: Catalytic subunit beta, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane whic\n Location: Mitochondrion inner membrane\n\nProtein 2: MMP25 (Q9NPA2)\n Function: May activate progelatinase A\n Location: Cell membrane\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between ATP5F1B and MMP25 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1881097, "gene_symbol_1": "ATP5F1B", "gene_symbol_2": "MMP25", "detection_method": null}} +{"question_id": "PPIL1-1071", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: ZNF704 (Q6ZNC4)\n Function: Transcription factor which binds to RE2 sequence elements in the MYOD1 enhancer\n Location: Nucleus\n\nProtein 2: M0R2Z0 (M0R2Z0)\n\nEvidence: No significant association between ZNF704 and None was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1782246, "gene_symbol_1": "ZNF704", "gene_symbol_2": null, "detection_method": null}} +{"question_id": "PPIL1-1072", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: PARD6G (Q9BYG4)\n Function: Adapter protein involved in asymmetrical cell division and cell polarization processes. May play a role in the formation of epithelial tight junctions. The PARD6-PARD3 complex links GTP-bound Rho smal\n Location: Cytoplasm\n\nProtein 2: ZDHHC6 (Q9H6R6)\n Function: Endoplasmic reticulum palmitoyl acyltransferase that mediates palmitoylation of proteins such as AMFR, CALX, ITPR1 and TFRC (PubMed:22314232, PubMed:22728137, PubMed:25368151, PubMed:28826475). Palmit\n Location: Endoplasmic reticulum membrane\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between PARD6G and ZDHHC6.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2222205, "gene_symbol_1": "PARD6G", "gene_symbol_2": "ZDHHC6", "detection_method": null}} +{"question_id": "PPIL1-1073", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: PLA2G2E (Q9NZK7)\n Function: Secretory calcium-dependent phospholipase A2 that primarily targets extracellular phospholipids (PubMed:10681567, PubMed:11922621, PubMed:28883454). Hydrolyzes the ester bond of the fatty acyl group a\n Location: Secreted\n\nProtein 2: ZNF716 (A6NP11)\n Function: May be involved in transcriptional regulation\n Location: Nucleus\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between PLA2G2E and ZNF716 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1756834, "gene_symbol_1": "PLA2G2E", "gene_symbol_2": "ZNF716", "detection_method": null}} +{"question_id": "PPIL1-1074", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: GTF2E2 (P29084)\n Function: Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clea\n Location: Nucleus\n\nProtein 2: CLXN (Q9HAE3)\n Function: Component of the outer dynein arm-docking complex (ODA-DC) that mediates outer dynein arms (ODA) binding onto the doublet microtubule. Seems to regulate the assembly of both ODAs and their axonemal do\n Location: Cytoplasm, cytoskeleton, cilium axoneme\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between GTF2E2 and CLXN.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1939646, "gene_symbol_1": "GTF2E2", "gene_symbol_2": "CLXN", "detection_method": null}} +{"question_id": "PPIL1-1075", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: ATP5MJ (P56378)\n Function: Subunit j, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generate\n Location: Mitochondrion membrane\n\nProtein 2: ZNF483 (Q8TF39)\n Function: May be involved in transcriptional regulation\n Location: Nucleus\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between ATP5MJ and ZNF483.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1999835, "gene_symbol_1": "ATP5MJ", "gene_symbol_2": "ZNF483", "detection_method": null}} +{"question_id": "PPIL1-1076", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: ZDHHC18 (Q9NUE0)\n Function: Palmitoyltransferase that catalyzes the addition of palmitate onto various protein substrates, such as CGAS, HRAS and LCK (PubMed:23034182, PubMed:27481942, PubMed:35438208). Acts as a negative regula\n Location: Golgi apparatus membrane\n\nProtein 2: ECT2 (Q9H8V3)\n Function: Guanine nucleotide exchange factor (GEF) that catalyzes the exchange of GDP for GTP. Promotes guanine nucleotide exchange on the Rho family members of small GTPases, like RHOA, RHOC, RAC1 and CDC42. R\n Location: Nucleus\n\nEvidence: No significant association between ZDHHC18 and ECT2 was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2229213, "gene_symbol_1": "ZDHHC18", "gene_symbol_2": "ECT2", "detection_method": null}} +{"question_id": "PPIL1-1077", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: ESM1 (Q9NQ30)\n Function: Involved in angiogenesis; promotes angiogenic sprouting. May have potent implications in lung endothelial cell-leukocyte interactions\n Location: Secreted\n\nProtein 2: RNF32 (Q9H0A6)\n Function: May play a role in sperm formation\n Location: Cytoplasm\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between ESM1 and RNF32 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2225024, "gene_symbol_1": "ESM1", "gene_symbol_2": "RNF32", "detection_method": null}} +{"question_id": "PPIL1-1078", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: STC1 (P52823)\n Function: Stimulates renal phosphate reabsorption, and could therefore prevent hypercalcemia\n Location: Secreted\n\nProtein 2: GRPEL2 (Q8TAA5)\n Function: Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner\n Location: Mitochondrion matrix\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between STC1 and GRPEL2 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1992594, "gene_symbol_1": "STC1", "gene_symbol_2": "GRPEL2", "detection_method": null}} +{"question_id": "PPIL1-1079", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: PPP1R37 (O75864)\n Function: Inhibits phosphatase activity of protein phosphatase 1 (PP1) complexes\n\nProtein 2: BSG (P35613)\n Function: Essential for normal retinal maturation and development (By similarity). Acts as a retinal cell surface receptor for NXNL1 and plays an important role in NXNL1-mediated survival of retinal cone photor\n Location: Melanosome\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between PPP1R37 and BSG is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1842971, "gene_symbol_1": "PPP1R37", "gene_symbol_2": "BSG", "detection_method": null}} +{"question_id": "PPIL1-1080", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: CBFA2T2 (O43439)\n Function: Transcriptional corepressor which facilitates transcriptional repression via its association with DNA-binding transcription factors and recruitment of other corepressors and histone-modifying enzymes \n Location: Nucleus\n\nProtein 2: MLIP (Q5VWP3)\n Function: Required for myoblast differentiation into myotubes, possibly acting as a transcriptional regulator of the myogenic program (By similarity). Required for cardiac adaptation to stress through integrate\n Location: Nucleus\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between CBFA2T2 and MLIP is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1813044, "gene_symbol_1": "CBFA2T2", "gene_symbol_2": "MLIP", "detection_method": null}} +{"question_id": "PPIL1-1081", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: UNC5B (Q8IZJ1)\n Function: Receptor for netrin required for axon guidance. Mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding. Axon repulsion in growth cones may be caused by i\n Location: Cell membrane\n\nProtein 2: GVQW3 (Q3ZCU0)\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between UNC5B and GVQW3 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2077987, "gene_symbol_1": "UNC5B", "gene_symbol_2": "GVQW3", "detection_method": null}} +{"question_id": "PPIL1-1082", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: RELA (Q04206)\n Function: NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to\n Location: Nucleus\n\nProtein 2: PDIA3 (P30101)\n Function: Protein disulfide isomerase that catalyzes the formation, isomerization, and reduction or oxidation of disulfide bonds in client proteins and functions as a protein folding chaperone (PubMed:11825568,\n Location: Endoplasmic reticulum\n\nEvidence: No significant association between RELA and PDIA3 was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1941988, "gene_symbol_1": "RELA", "gene_symbol_2": "PDIA3", "detection_method": null}} +{"question_id": "PPIL1-1083", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: MYCL (P12524)\n Location: Nucleus\n\nProtein 2: ZNF763 (K7ESF6)\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between MYCL and ZNF763.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1781077, "gene_symbol_1": "MYCL", "gene_symbol_2": "ZNF763", "detection_method": null}} +{"question_id": "PPIL1-1084", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: MTFR1 (Q15390)\n Function: May play a role in mitochondrial aerobic respiration. May also regulate mitochondrial organization and fission (By similarity)\n Location: Mitochondrion\n\nProtein 2: TMEM98 (Q9Y2Y6)\n Function: Functions as a negative regulator of MYRF in oligodendrocyte differentiation and myelination. Interacts with the C-terminal of MYRF inhibiting MYRF self-cleavage and N-fragment nuclear translocation. \n Location: Cell membrane\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between MTFR1 and TMEM98.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2059793, "gene_symbol_1": "MTFR1", "gene_symbol_2": "TMEM98", "detection_method": null}} +{"question_id": "PPIL1-1085", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: SAR1B (Q9Y6B6)\n Function: Small GTPase that cycles between an active GTP-bound and an inactive GDP-bound state and mainly functions in vesicle-mediated endoplasmic reticulum (ER) to Golgi transport. The active GTP-bound form i\n Location: Endoplasmic reticulum membrane\n\nProtein 2: EIF3CL (B5ME19)\n Function: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribos\n Location: Cytoplasm\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between SAR1B and EIF3CL is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1764941, "gene_symbol_1": "SAR1B", "gene_symbol_2": "EIF3CL", "detection_method": null}} +{"question_id": "PPIL1-1086", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: ZMAT4 (Q9H898)\n Location: Nucleus\n\nProtein 2: NPHS2 (Q9NP85)\n Function: Plays a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton\n Location: Cell membrane\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between ZMAT4 and NPHS2 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2229065, "gene_symbol_1": "ZMAT4", "gene_symbol_2": "NPHS2", "detection_method": null}} +{"question_id": "PPIL1-1087", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: PRAMEF10 (O60809)\n\nProtein 2: ARHGAP15 (Q53QZ3)\n Function: GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state. Has activity toward RAC1. Overexpression results in an increase in actin stress fibers and cell contraction\n Location: Cytoplasm\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between PRAMEF10 and ARHGAP15.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1827995, "gene_symbol_1": "PRAMEF10", "gene_symbol_2": "ARHGAP15", "detection_method": null}} +{"question_id": "PPIL1-1088", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: SHROOM2 (Q13796)\n Function: May be involved in endothelial cell morphology changes during cell spreading. In the retinal pigment epithelium, may regulate the biogenesis of melanosomes and promote their association with the apica\n Location: Apical cell membrane\n\nProtein 2: ZNF737 (O75373)\n Function: May be involved in transcriptional regulation\n Location: Nucleus\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between SHROOM2 and ZNF737.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1835787, "gene_symbol_1": "SHROOM2", "gene_symbol_2": "ZNF737", "detection_method": null}} +{"question_id": "PPIL1-1089", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: AQP8 (O94778)\n Function: Channel that allows the facilitated permeation of water and uncharged molecules, such as hydrogen peroxide and the neutral form of ammonia (NH3), through cellular membranes such as plasma membrane, in\n Location: Cell membrane\n\nProtein 2: BOLA1 (Q9Y3E2)\n Function: Acts as a mitochondrial iron-sulfur (Fe-S) cluster assembly factor that facilitates (Fe-S) cluster insertion into a subset of mitochondrial proteins (By similarity). Probably acts together with the mo\n Location: Mitochondrion\n\nEvidence: No significant association between AQP8 and BOLA1 was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1847739, "gene_symbol_1": "AQP8", "gene_symbol_2": "BOLA1", "detection_method": null}} +{"question_id": "PPIL1-1090", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: FHL3 (Q13643)\n Function: Recruited by SOX15 to FOXK1 promoters where it acts as a transcriptional coactivator of FOXK1\n Location: Nucleus\n\nProtein 2: CAPZA2 (P47755)\n Function: F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping prot\n\nEvidence: No significant association between FHL3 and CAPZA2 was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1976221, "gene_symbol_1": "FHL3", "gene_symbol_2": "CAPZA2", "detection_method": null}} +{"question_id": "PPIL1-1091", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: PLSCR1 (O15162)\n Function: Catalyzes calcium-induced ATP-independent rapid bidirectional and non-specific movement of phospholipids (lipid scrambling or lipid flip-flop) between the inner and outer leaflet of the plasma membran\n Location: Cell membrane\n\nProtein 2: RPS12 (P25398)\n Function: Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an \n Location: Cytoplasm\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between PLSCR1 and RPS12 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1802348, "gene_symbol_1": "PLSCR1", "gene_symbol_2": "RPS12", "detection_method": null}} +{"question_id": "PPIL1-1092", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: MACROD1 (Q9BQ69)\n Function: Removes ADP-ribose from aspartate and glutamate residues in proteins bearing a single ADP-ribose moiety (PubMed:23474712, PubMed:23474714). Inactive towards proteins bearing poly-ADP-ribose (PubMed:23\n Location: Nucleus\n\nProtein 2: HAPLN4 (Q86UW8)\n Function: Essential for the proper localization of brevican (BCAN), mainly as a perineuronal nets (PNNs)-type deposition in the brainstem and cerebellum thereby playing a key role in the formation and structura\n Location: Secreted, extracellular space, extracellular matrix\n\nEvidence: No significant association between MACROD1 and HAPLN4 was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2144181, "gene_symbol_1": "MACROD1", "gene_symbol_2": "HAPLN4", "detection_method": null}} +{"question_id": "PPIL1-1093", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: GDF9 (O60383)\n Function: Required for ovarian folliculogenesis. Promotes primordial follicle development. Stimulates granulosa cell proliferation. Promotes cell transition from G0/G1 to S and G2/M phases, through an increase \n Location: Secreted\n\nProtein 2: UNK (Q9C0B0)\n Function: Sequence-specific RNA-binding protein which plays an important role in the establishment and maintenance of the early morphology of cortical neurons during embryonic development. Acts as a translation\n Location: Cytoplasm\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between GDF9 and UNK is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1823214, "gene_symbol_1": "GDF9", "gene_symbol_2": "UNK", "detection_method": null}} +{"question_id": "PPIL1-1094", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: OR8K5 (Q8NH50)\n Function: Odorant receptor\n Location: Cell membrane\n\nProtein 2: FAM216A (Q8WUB2)\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between OR8K5 and FAM216A is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2180230, "gene_symbol_1": "OR8K5", "gene_symbol_2": "FAM216A", "detection_method": null}} +{"question_id": "PPIL1-1095", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: SLC18A1 (P54219)\n Function: Electrogenic antiporter that exchanges one cationic monoamine with two intravesicular protons across the membrane of secretory and synaptic vesicles. Uses the electrochemical proton gradient establish\n Location: Cytoplasmic vesicle, secretory vesicle membrane\n\nProtein 2: RNF114 (Q9Y508)\n Function: E3 ubiquitin-protein ligase that promotes the ubiquitination of various substrates (PubMed:23645206, PubMed:25165885). In turn, participates in the regulation of many biological processes including ce\n Location: Cytoplasm\n\nEvidence: No significant association between SLC18A1 and RNF114 was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1995036, "gene_symbol_1": "SLC18A1", "gene_symbol_2": "RNF114", "detection_method": null}} +{"question_id": "PPIL1-1096", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: NCAPD2 (Q15021)\n Function: Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive superc\n Location: Nucleus\n\nProtein 2: CHID1 (Q9BWS9)\n Function: Saccharide- and LPS-binding protein with possible roles in pathogen sensing and endotoxin neutralization. Ligand-binding specificity relates to the length of the oligosaccharides, with preference for \n Location: Secreted\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between NCAPD2 and CHID1.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2056657, "gene_symbol_1": "NCAPD2", "gene_symbol_2": "CHID1", "detection_method": null}} +{"question_id": "PPIL1-1097", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: SPANXN2 (Q5MJ10)\n\nProtein 2: MAML3 (Q96JK9)\n Function: Acts as a transcriptional coactivator for NOTCH proteins. Has been shown to amplify NOTCH-induced transcription of HES1\n Location: Nucleus speckle\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between SPANXN2 and MAML3.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2090525, "gene_symbol_1": "SPANXN2", "gene_symbol_2": "MAML3", "detection_method": null}} +{"question_id": "PPIL1-1098", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: POU2F2 (P09086)\n Function: Transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3') (PubMed:2904654, PubMed:7859290). Regulates IL6 expression in B cells with POU2AF1 (By similarity). Regulates transcr\n Location: Cytoplasm\n\nProtein 2: SMIM12 (Q96EX1)\n Location: Membrane\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between POU2F2 and SMIM12 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1888086, "gene_symbol_1": "POU2F2", "gene_symbol_2": "SMIM12", "detection_method": null}} +{"question_id": "PPIL1-1099", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: TSBP1 (Q5SRN2)\n Location: Membrane\n\nProtein 2: ADH5 (P11766)\n Function: Catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione (PubMed:8460164). Also oxidizes long chain omega-hydroxy fatty acids, such as 20-HETE, produci\n Location: Cytoplasm\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between TSBP1 and ADH5 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1902986, "gene_symbol_1": "TSBP1", "gene_symbol_2": "ADH5", "detection_method": null}} +{"question_id": "PPIL1-1100", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: VHLL (Q6RSH7)\n Function: Functions as a dominant-negative VHL to serve as a protector of HIFalpha\n\nProtein 2: BRPF3 (Q9ULD4)\n Function: Scaffold subunit of various histone acetyltransferase (HAT) complexes, such as the MOZ/MORF and HBO1 complexes, which have a histone H3 acetyltransferase activity (PubMed:16387653, PubMed:26620551, Pu\n Location: Nucleus\n\nEvidence: No significant association between VHLL and BRPF3 was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2119186, "gene_symbol_1": "VHLL", "gene_symbol_2": "BRPF3", "detection_method": null}} +{"question_id": "PPIL1-1101", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: TBC1D14 (Q9P2M4)\n Function: Plays a role in the regulation of starvation-induced autophagosome formation (PubMed:22613832). Together with the TRAPPIII complex, regulates a constitutive trafficking step from peripheral recycling \n Location: Golgi apparatus, cis-Golgi network\n\nProtein 2: FABP6 (P51161)\n Function: Binds to bile acids and is involved in enterohepatic bile acid metabolism. Required for efficient apical to basolateral transport of conjugated bile acids in ileal enterocytes (By similarity). In vitr\n Location: Cytoplasm\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between TBC1D14 and FABP6.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1987200, "gene_symbol_1": "TBC1D14", "gene_symbol_2": "FABP6", "detection_method": null}} +{"question_id": "PPIL1-1102", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: SELENOI (Q9C0D9)\n Function: Ethanolaminephosphotransferase that catalyzes the transfer of phosphoethanolamine (PE) from CDP-ethanolamine to lipid acceptors, the final step in the synthesis of PE via the 'Kennedy' pathway (PubMed\n Location: Endoplasmic reticulum membrane\n\nProtein 2: MGA (Q8IWI9)\n Function: Functions as a dual-specificity transcription factor, regulating the expression of both MAX-network and T-box family target genes. Functions as a repressor or an activator. Binds to 5'-AATTTCACACCTAGG\n Location: Nucleus\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between SELENOI and MGA.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2153541, "gene_symbol_1": "SELENOI", "gene_symbol_2": "MGA", "detection_method": null}} +{"question_id": "PPIL1-1103", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: MAST2 (Q6P0Q8)\n Function: Appears to link the dystrophin/utrophin network with microtubule filaments via the syntrophins. Phosphorylation of DMD or UTRN may modulate their affinities for associated proteins. Functions in a mul\n Location: Cytoplasm, cytoskeleton\n\nProtein 2: MYCBP2 (O75592)\n Function: Atypical E3 ubiquitin-protein ligase which specifically mediates ubiquitination of threonine and serine residues on target proteins, instead of ubiquitinating lysine residues (PubMed:29643511). Shows \n Location: Nucleus\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between MAST2 and MYCBP2 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1839560, "gene_symbol_1": "MAST2", "gene_symbol_2": "MYCBP2", "detection_method": null}} +{"question_id": "PPIL1-1104", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: HTR2C (P28335)\n Function: G-protein coupled receptor for 5-hydroxytryptamine (serotonin) (PubMed:12970106, PubMed:18703043, PubMed:19057895, PubMed:29398112, PubMed:7895773). Also functions as a receptor for various drugs and \n Location: Cell membrane\n\nProtein 2: C1orf210 (Q8IVY1)\n Function: May be involved in membrane trafficking between endosomes and plasma membrane\n Location: Membrane\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between HTR2C and C1orf210.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1938425, "gene_symbol_1": "HTR2C", "gene_symbol_2": "C1orf210", "detection_method": null}} +{"question_id": "PPIL1-1105", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: SELENBP1 (Q13228)\n Function: Catalyzes the oxidation of methanethiol, an organosulfur compound known to be produced in substantial amounts by gut bacteria (PubMed:29255262). Selenium-binding protein which may be involved in the s\n Location: Nucleus\n\nProtein 2: ZNHIT3 (Q15649)\n Location: Cytoplasm\n\nEvidence: No significant association between SELENBP1 and ZNHIT3 was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2040193, "gene_symbol_1": "SELENBP1", "gene_symbol_2": "ZNHIT3", "detection_method": null}} +{"question_id": "PPIL1-1106", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: THSD4 (Q6ZMP0)\n Function: Promotes FBN1 matrix assembly. Attenuates TGFB signaling, possibly by accelerating the sequestration of large latent complexes of TGFB or active TGFB by FBN1 microfibril assembly, thereby negatively r\n Location: Secreted, extracellular space, extracellular matrix\n\nProtein 2: NLRX1 (Q86UT6)\n Function: Participates in antiviral signaling. Acts as a negative regulator of MAVS-mediated antiviral responses, through the inhibition of the virus-induced RLH (RIG-like helicase)-MAVS interaction (PubMed:182\n Location: Mitochondrion outer membrane\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between THSD4 and NLRX1.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2125615, "gene_symbol_1": "THSD4", "gene_symbol_2": "NLRX1", "detection_method": null}} +{"question_id": "PPIL1-1107", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: SCAMP2 (O15127)\n Function: Functions in post-Golgi recycling pathways. Acts as a recycling carrier to the cell surface\n Location: Golgi apparatus, trans-Golgi network membrane\n\nProtein 2: GEM (P55040)\n Function: Could be a regulatory protein, possibly participating in receptor-mediated signal transduction at the plasma membrane. Has guanine nucleotide-binding activity but undetectable intrinsic GTPase activit\n Location: Cell membrane\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between SCAMP2 and GEM is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1801814, "gene_symbol_1": "SCAMP2", "gene_symbol_2": "GEM", "detection_method": null}} +{"question_id": "PPIL1-1108", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: YOD1 (Q5VVQ6)\n Function: Hydrolase that can remove conjugated ubiquitin from proteins and participates in endoplasmic reticulum-associated degradation (ERAD) for misfolded lumenal proteins. May act by triming the ubiquitin ch\n Location: Cytoplasm\n\nProtein 2: SRRM3 (A6NNA2)\n Function: May play a role in regulating breast cancer cell invasiveness (PubMed:26053433). May be involved in RYBP-mediated breast cancer progression (PubMed:27748911)\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between YOD1 and SRRM3 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1755946, "gene_symbol_1": "YOD1", "gene_symbol_2": "SRRM3", "detection_method": null}} +{"question_id": "PPIL1-1109", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: TPR (P12270)\n Function: Component of the nuclear pore complex (NPC), a complex required for the trafficking across the nuclear envelope. Functions as a scaffolding element in the nuclear phase of the NPC essential for normal\n Location: Nucleus\n\nProtein 2: DNAJC21 (Q5F1R6)\n Function: May act as a co-chaperone for HSP70. May play a role in ribosomal RNA (rRNA) biogenesis, possibly in the maturation of the 60S subunit. Binds the precursor 45S rRNA\n Location: Cytoplasm\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between TPR and DNAJC21.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1904181, "gene_symbol_1": "TPR", "gene_symbol_2": "DNAJC21", "detection_method": null}} +{"question_id": "PPIL1-1110", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: GPR162 (Q16538)\n Function: Orphan receptor\n Location: Cell membrane\n\nProtein 2: ATP1B2 (P14415)\n Function: This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The exact function of the b\n Location: Cell membrane\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between GPR162 and ATP1B2 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1908859, "gene_symbol_1": "GPR162", "gene_symbol_2": "ATP1B2", "detection_method": null}} +{"question_id": "PPIL1-1111", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: DFFB (O76075)\n Function: Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology\n Location: Cytoplasm\n\nProtein 2: KRTAP19-1 (Q8IUB9)\n Function: In the hair cortex, hair keratin intermediate filaments are embedded in an interfilamentous matrix, consisting of hair keratin-associated proteins (KRTAP), which are essential for the formation of a r\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between DFFB and KRTAP19-1 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1846707, "gene_symbol_1": "DFFB", "gene_symbol_2": "KRTAP19-1", "detection_method": null}} +{"question_id": "PPIL1-1112", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: OCIAD1 (Q9NX40)\n Function: Maintains stem cell potency (By similarity). Increases STAT3 phosphorylation and controls ERK phosphorylation (By similarity). May act as a scaffold, increasing STAT3 recruitment onto endosomes (By si\n Location: Endosome\n\nProtein 2: SUFU (Q9UMX1)\n Function: Negative regulator in the hedgehog/smoothened signaling pathway (PubMed:10559945, PubMed:10564661, PubMed:10806483, PubMed:12068298, PubMed:12975309, PubMed:15367681, PubMed:22365972, PubMed:24217340,\n Location: Cytoplasm\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between OCIAD1 and SUFU is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2234762, "gene_symbol_1": "OCIAD1", "gene_symbol_2": "SUFU", "detection_method": null}} +{"question_id": "PPIL1-1113", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: ZNF3 (P17036)\n Function: Involved in cell differentiation and/or proliferation\n Location: Nucleus\n\nProtein 2: ZNF781 (Q8N8C0)\n Function: May be involved in transcriptional regulation\n Location: Nucleus\n\nEvidence: No significant association between ZNF3 and ZNF781 was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1915479, "gene_symbol_1": "ZNF3", "gene_symbol_2": "ZNF781", "detection_method": null}} +{"question_id": "PPIL1-1114", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: KRT26 (Q7Z3Y9)\n\nProtein 2: MEFV (O15553)\n Function: Involved in the regulation of innate immunity and the inflammatory response in response to IFNG/IFN-gamma (PubMed:10807793, PubMed:11468188, PubMed:16037825, PubMed:16785446, PubMed:17431422, PubMed:1\n Location: Cytoplasm, cytoskeleton\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between KRT26 and MEFV is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1808605, "gene_symbol_1": "KRT26", "gene_symbol_2": "MEFV", "detection_method": null}} +{"question_id": "PPIL1-1115", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: FREM2 (Q5SZK8)\n Function: Extracellular matrix protein required for maintenance of the integrity of the skin epithelium and for maintenance of renal epithelia (PubMed:15838507). Required for epidermal adhesion (PubMed:15838507\n Location: Cell membrane\n\nProtein 2: PRAMEF17 (Q5VTA0)\n\nEvidence: No significant association between FREM2 and PRAMEF17 was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2092886, "gene_symbol_1": "FREM2", "gene_symbol_2": "PRAMEF17", "detection_method": null}} +{"question_id": "PPIL1-1116", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: CD300LG (Q6UXG3)\n Function: Receptor which may mediate L-selectin-dependent lymphocyte rollings. Binds SELL in a calcium dependent manner. Binds lymphocyte (By similarity)\n Location: Apical cell membrane\n\nProtein 2: ZNRF3 (Q9ULT6)\n Function: E3 ubiquitin-protein ligase that acts as a negative regulator of the Wnt signaling pathway by mediating the ubiquitination and subsequent degradation of Wnt receptor complex components Frizzled and LR\n Location: Cell membrane\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between CD300LG and ZNRF3.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2122812, "gene_symbol_1": "CD300LG", "gene_symbol_2": "ZNRF3", "detection_method": null}} +{"question_id": "PPIL1-1117", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: MCM6 (Q14566)\n Function: Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core compon\n Location: Nucleus\n\nProtein 2: ABHD15 (Q6UXT9)\n Function: May regulate adipocyte lipolysis and liver lipid accumulation\n Location: Secreted\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between MCM6 and ABHD15 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2052136, "gene_symbol_1": "MCM6", "gene_symbol_2": "ABHD15", "detection_method": null}} +{"question_id": "PPIL1-1118", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: PIGZ (Q86VD9)\n Function: Alpha-1,2-mannosyltransferase that catalyzes the transfer of the fourth mannose, via an alpha-1,2 bond, from a dolichol-phosphate-mannose (Dol-P-Man) to a 2-acyl-6-[alpha-D-mannosyl-(1->2)-alpha-D-man\n Location: Endoplasmic reticulum membrane\n\nProtein 2: OR13C4 (Q8NGS5)\n Function: Odorant receptor\n Location: Cell membrane\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between PIGZ and OR13C4 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2144897, "gene_symbol_1": "PIGZ", "gene_symbol_2": "OR13C4", "detection_method": null}} +{"question_id": "PPIL1-1119", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: SLC6A16 (Q9GZN6)\n Location: Membrane\n\nProtein 2: GYPE (P15421)\n Function: This protein is a minor sialoglycoprotein in human erythrocyte membranes\n Location: Membrane\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between SLC6A16 and GYPE.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1911497, "gene_symbol_1": "SLC6A16", "gene_symbol_2": "GYPE", "detection_method": null}} +{"question_id": "PPIL1-1120", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: SNRNP35 (Q16560)\n Location: Nucleus\n\nProtein 2: MIOX (Q9UGB7)\n Location: Cytoplasm\n\nEvidence: No significant association between SNRNP35 and MIOX was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2065761, "gene_symbol_1": "SNRNP35", "gene_symbol_2": "MIOX", "detection_method": null}} +{"question_id": "PPIL1-1121", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: HADHB (P55084)\n Function: Mitochondrial trifunctional enzyme catalyzes the last three of the four reactions of the mitochondrial beta-oxidation pathway (PubMed:29915090, PubMed:30850536, PubMed:8135828). The mitochondrial beta\n Location: Mitochondrion\n\nProtein 2: JMJD1C (Q15652)\n Function: Demethylates lysine in proteins, such as STAT3 or MDC1 (By similarity) (PubMed:24240613). Demethylates MDC1, thereby promoting MDC1-RNF8 interaction and facilitating RNF8-dependent MDC1 ubiquitination\n Location: Nucleus\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between HADHB and JMJD1C is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1997486, "gene_symbol_1": "HADHB", "gene_symbol_2": "JMJD1C", "detection_method": null}} +{"question_id": "PPIL1-1122", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: ANXA10 (Q9UJ72)\n\nProtein 2: USP17L5 (A8MUK1)\n Function: Deubiquitinating enzyme that removes conjugated ubiquitin from specific proteins to regulate different cellular processes that may include cell proliferation, progression through the cell cycle, apopt\n Location: Nucleus\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between ANXA10 and USP17L5 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1760010, "gene_symbol_1": "ANXA10", "gene_symbol_2": "USP17L5", "detection_method": null}} +{"question_id": "PPIL1-1123", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: MPLKIP (Q8TAP9)\n Function: May play a role in maintenance of cell cycle integrity by regulating mitosis or cytokinesis\n Location: Nucleus\n\nProtein 2: ANKRD34C (P0C6C1)\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between MPLKIP and ANKRD34C.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1891993, "gene_symbol_1": "MPLKIP", "gene_symbol_2": "ANKRD34C", "detection_method": null}} +{"question_id": "PPIL1-1124", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: UBR5 (O95071)\n Function: E3 ubiquitin-protein ligase involved in different protein quality control pathways in the cytoplasm and nucleus (PubMed:29033132, PubMed:33208877, PubMed:37478846, PubMed:37478862). Mainly acts as a u\n Location: Nucleus\n\nProtein 2: ADCY2 (Q08462)\n Function: Catalyzes the formation of the signaling molecule cAMP in response to G-protein signaling (PubMed:15385642). Down-stream signaling cascades mediate changes in gene expression patterns and lead to incr\n Location: Membrane\n\nEvidence: No significant association between UBR5 and ADCY2 was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1852008, "gene_symbol_1": "UBR5", "gene_symbol_2": "ADCY2", "detection_method": null}} +{"question_id": "PPIL1-1125", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: THAP11 (Q96EK4)\n Function: Transcription factor, which has both transcriptional activation and repression activities (PubMed:31905202). Also modulates chromatin accessibility (PubMed:38361031). In complex with HCFC1 and ZNF143,\n Location: Nucleus\n\nProtein 2: FGFR3 (P22607)\n Function: Tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation and apoptosis. Plays an \n Location: Cell membrane\n\nEvidence: No significant association between THAP11 and FGFR3 was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1928159, "gene_symbol_1": "THAP11", "gene_symbol_2": "FGFR3", "detection_method": null}} +{"question_id": "PPIL1-1126", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: COA7 (Q96BR5)\n Function: Required for assembly of mitochondrial respiratory chain complex I and complex IV\n Location: Mitochondrion intermembrane space\n\nProtein 2: FNBP4 (Q8N3X1)\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between COA7 and FNBP4 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2163542, "gene_symbol_1": "COA7", "gene_symbol_2": "FNBP4", "detection_method": null}} +{"question_id": "PPIL1-1127", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: URGCP (Q8TCY9)\n Function: May be involved in cell cycle progression through the regulation of cyclin D1 expression. May participate in the development of hepatocellular carcinoma (HCC) by promoting hepatocellular growth and su\n Location: Cytoplasm\n\nProtein 2: EEF2 (P13639)\n Function: Catalyzes the GTP-dependent ribosomal translocation step during translation elongation (PubMed:26593721). During this step, the ribosome changes from the pre-translocational (PRE) to the post-transloc\n Location: Cytoplasm\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between URGCP and EEF2.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1906946, "gene_symbol_1": "URGCP", "gene_symbol_2": "EEF2", "detection_method": null}} +{"question_id": "PPIL1-1128", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: STX12 (Q86Y82)\n Function: SNARE promoting fusion of transport vesicles with target membranes. Together with SNARE STX6, promotes movement of vesicles from endosomes to the cell membrane, and may therefore function in the endoc\n Location: Endosome membrane\n\nProtein 2: VMP1 (Q96GC9)\n Function: Phospholipid scramblase involved in lipid homeostasis and membrane dynamics processes (PubMed:33850023, PubMed:33929485). Has phospholipid scramblase activity toward cholesterol and phosphatidylserine\n Location: Endoplasmic reticulum-Golgi intermediate compartment membrane\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between STX12 and VMP1.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2149075, "gene_symbol_1": "STX12", "gene_symbol_2": "VMP1", "detection_method": null}} +{"question_id": "PPIL1-1129", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: PFKP (Q01813)\n Function: Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis\n Location: Cytoplasm\n\nProtein 2: USP17L10 (C9JJH3)\n Function: Deubiquitinating enzyme that removes conjugated ubiquitin from specific proteins to regulate different cellular processes that may include cell proliferation, progression through the cell cycle, apopt\n Location: Nucleus\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between PFKP and USP17L10.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1767469, "gene_symbol_1": "PFKP", "gene_symbol_2": "USP17L10", "detection_method": null}} +{"question_id": "PPIL1-1130", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: STATH (P02808)\n Function: Salivary protein that stabilizes saliva supersaturated with calcium salts by inhibiting the precipitation of calcium phosphate salts. It also modulates hydroxyapatite crystal formation on the tooth su\n Location: Secreted\n\nProtein 2: ZNF671 (Q8TAW3)\n Function: May be involved in transcriptional regulation\n Location: Nucleus\n\nEvidence: No significant association between STATH and ZNF671 was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1873802, "gene_symbol_1": "STATH", "gene_symbol_2": "ZNF671", "detection_method": null}} +{"question_id": "PPIL1-1131", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: EPDR1 (Q9UM22)\n Function: Binds anionic lipids and gangliosides at acidic pH\n Location: Lysosome lumen\n\nProtein 2: UROS (P10746)\n Function: Catalyzes cyclization of the linear tetrapyrrole, hydroxymethylbilane, to the macrocyclic uroporphyrinogen III, the branch point for the various sub-pathways leading to the wide diversity of porphyrin\n Location: Cytoplasm, cytosol\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between EPDR1 and UROS is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1900225, "gene_symbol_1": "EPDR1", "gene_symbol_2": "UROS", "detection_method": null}} +{"question_id": "PPIL1-1132", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: LHX2 (P50458)\n Function: Acts as a transcriptional activator. Stimulates the promoter of the alpha-glycoprotein gene. Transcriptional regulatory protein involved in the control of cell differentiation in developing lymphoid a\n Location: Nucleus\n\nProtein 2: COL20A1 (Q9P218)\n Function: Probable collagen protein\n Location: Secreted, extracellular space\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between LHX2 and COL20A1.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1985600, "gene_symbol_1": "LHX2", "gene_symbol_2": "COL20A1", "detection_method": null}} +{"question_id": "PPIL1-1133", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: ARMH2 (H3BNL8)\n\nProtein 2: PIEZO1 (Q92508)\n Function: Pore-forming subunit of the mechanosensitive non-specific cation Piezo channel required for rapidly adapting mechanically activated (MA) currents and has a key role in sensing touch and tactile pain (\n Location: Endoplasmic reticulum membrane\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between ARMH2 and PIEZO1 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1776336, "gene_symbol_1": "ARMH2", "gene_symbol_2": "PIEZO1", "detection_method": null}} +{"question_id": "PPIL1-1134", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: TRIP10 (Q15642)\n Function: Required for translocation of GLUT4 to the plasma membrane in response to insulin signaling (By similarity). Required to coordinate membrane tubulation with reorganization of the actin cytoskeleton du\n Location: Cytoplasm, cytoskeleton\n\nProtein 2: OR10P1 (Q8NGE3)\n Function: Odorant receptor\n Location: Cell membrane\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between TRIP10 and OR10P1.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2061793, "gene_symbol_1": "TRIP10", "gene_symbol_2": "OR10P1", "detection_method": null}} +{"question_id": "PPIL1-1135", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: NEFM (P07197)\n Function: Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal interm\n Location: Cytoplasm, cytoskeleton\n\nProtein 2: GALNT4 (Q8N4A0)\n Function: Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Has a highest act\n Location: Golgi apparatus membrane\n\nEvidence: No significant association between NEFM and GALNT4 was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1882154, "gene_symbol_1": "NEFM", "gene_symbol_2": "GALNT4", "detection_method": null}} +{"question_id": "PPIL1-1136", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: PACC1 (Q9H813)\n Function: Chloride channel gated by pH that facilitates the entry of chloride ions into cells upon exposure to extracellular acidic pH (PubMed:31023925, PubMed:31318332). Involved in acidosis-induced cell death\n Location: Cell membrane\n\nProtein 2: SC5D (O75845)\n Function: Catalyzes the penultimate step of the biosynthesis of cholesterol, the dehydrogenation of lathosterol into 7-dehydrocholesterol (7-DHC). Cholesterol is the major sterol component in mammalian membrane\n Location: Endoplasmic reticulum membrane\n\nEvidence: No significant association between PACC1 and SC5D was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1842948, "gene_symbol_1": "PACC1", "gene_symbol_2": "SC5D", "detection_method": null}} +{"question_id": "PPIL1-1137", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: RBL2 (Q08999)\n Function: Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabili\n Location: Nucleus\n\nProtein 2: ZBED3 (Q96IU2)\n Function: Acts as a positive regulator in the activation of the canonical Wnt/beta-catenin signaling pathway by stabilizing cytoplasmic beta-catenin (By similarity). Involved in transcription activation of Wnt \n Location: Cytoplasm\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between RBL2 and ZBED3 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2031655, "gene_symbol_1": "RBL2", "gene_symbol_2": "ZBED3", "detection_method": null}} +{"question_id": "PPIL1-1138", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: RABL6 (Q3YEC7)\n Function: Small GTPase involved in the regulation of cell growth and survival (PubMed:19433581). Promotes cellular proliferation (PubMed:16582619, PubMed:19433581). May reduce growth inhibitory activity of CDKN\n Location: Cytoplasm\n\nProtein 2: HDGF (P51858)\n Function: Acts as a transcriptional repressor (PubMed:17974029). Has mitogenic activity for fibroblasts (PubMed:11751870, PubMed:26845719). Heparin-binding protein (PubMed:15491618)\n Location: Nucleus\n\nEvidence: No significant association between RABL6 and HDGF was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1990388, "gene_symbol_1": "RABL6", "gene_symbol_2": "HDGF", "detection_method": null}} +{"question_id": "PPIL1-1139", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: OR1J2 (Q8NGS2)\n Function: Odorant receptor\n Location: Cell membrane\n\nProtein 2: FAM162B (Q5T6X4)\n Location: Membrane\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between OR1J2 and FAM162B is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2095567, "gene_symbol_1": "OR1J2", "gene_symbol_2": "FAM162B", "detection_method": null}} +{"question_id": "PPIL1-1140", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: MNT (Q99583)\n Function: Binds DNA as a heterodimer with MAX and represses transcription. Binds to the canonical E box sequence 5'-CACGTG-3' and, with higher affinity, to 5'-CACGCG-3'\n Location: Nucleus\n\nProtein 2: PRDM16 (Q9HAZ2)\n Function: Transcription regulator that acts both as a histone methyltransferase or chromatin adapter, depending on the context (PubMed:12816872). In the cytoplasm, acts as a histone methyltransferase, which cat\n Location: Nucleus\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between MNT and PRDM16 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2214478, "gene_symbol_1": "MNT", "gene_symbol_2": "PRDM16", "detection_method": null}} +{"question_id": "PPIL1-1141", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: DAO (P14920)\n Function: Catalyzes the oxidative deamination of D-amino acids with broad substrate specificity (PubMed:16616139, PubMed:17088322, PubMed:17303072, PubMed:18544534, PubMed:20368421, PubMed:20567862, PubMed:2060\n Location: Peroxisome matrix\n\nProtein 2: RALYL (Q86SE5)\n\nEvidence: No significant association between DAO and RALYL was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1910057, "gene_symbol_1": "DAO", "gene_symbol_2": "RALYL", "detection_method": null}} +{"question_id": "PPIL1-1142", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: TMEM19 (Q96HH6)\n Location: Membrane\n\nProtein 2: MTF2 (Q9Y483)\n Function: Polycomb group (PcG) protein that specifically binds histone H3 trimethylated at 'Lys-36' (H3K36me3) and recruits the PRC2 complex, thus enhancing PRC2 H3K27me3 methylation activity (PubMed:23142980, \n Location: Nucleus\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between TMEM19 and MTF2 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2204964, "gene_symbol_1": "TMEM19", "gene_symbol_2": "MTF2", "detection_method": null}} +{"question_id": "PPIL1-1143", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: GPR42 (O15529)\n Function: G protein-coupled receptor that is activated by short chain fatty acids (SCFAs), such as propionate. Hence may play a role in the regulation of whole-body energy homeostasis and/or in intestinal immun\n Location: Cell membrane\n\nProtein 2: PLA2G3 (Q9NZ20)\n Function: Secretory calcium-dependent phospholipase A2 that primarily targets extracellular phospholipids. Hydrolyzes the ester bond of the fatty acyl group attached at sn-2 position of phospholipids without ap\n Location: Secreted\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between GPR42 and PLA2G3.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1808024, "gene_symbol_1": "GPR42", "gene_symbol_2": "PLA2G3", "detection_method": null}} +{"question_id": "PPIL1-1144", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: KRTAP6-2 (Q3LI66)\n Function: In the hair cortex, hair keratin intermediate filaments are embedded in an interfilamentous matrix, consisting of hair keratin-associated proteins (KRTAP), which are essential for the formation of a r\n\nProtein 2: ARHGAP17 (Q68EM7)\n Function: Rho GTPase-activating protein involved in the maintenance of tight junction by regulating the activity of CDC42, thereby playing a central role in apical polarity of epithelial cells. Specifically act\n Location: Membrane\n\nEvidence: No significant association between KRTAP6-2 and ARHGAP17 was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2075948, "gene_symbol_1": "KRTAP6-2", "gene_symbol_2": "ARHGAP17", "detection_method": null}} +{"question_id": "PPIL1-1145", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: RTP2 (Q5QGT7)\n Function: Specifically promotes functional cell surface expression of olfactory receptors, but not of other GPCRs\n Location: Cell membrane\n\nProtein 2: GOLGA8N (F8WBI6)\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between RTP2 and GOLGA8N is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1773417, "gene_symbol_1": "RTP2", "gene_symbol_2": "GOLGA8N", "detection_method": null}} +{"question_id": "PPIL1-1146", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: PSMD5 (Q16401)\n Function: Acts as a chaperone during the assembly of the 26S proteasome, specifically of the base subcomplex of the PA700/19S regulatory complex (RC). In the initial step of the base subcomplex assembly is part\n\nProtein 2: HOXA10-HOXA9 (D6RAR5)\n Location: Nucleus\n\nEvidence: No significant association between PSMD5 and HOXA10-HOXA9 was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1768884, "gene_symbol_1": "PSMD5", "gene_symbol_2": "HOXA10-HOXA9", "detection_method": null}} +{"question_id": "PPIL1-1147", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: REPS1 (Q96D71)\n Function: May coordinate the cellular actions of activated EGF receptors and Ral-GTPases\n Location: Membrane, clathrin-coated pit\n\nProtein 2: GPX4 (P36969)\n Function: Essential antioxidant peroxidase that directly reduces phospholipid hydroperoxide even if they are incorporated in membranes and lipoproteins (PubMed:40281343). Can also reduce cholesterol hydroperoxi\n Location: Mitochondrion\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between REPS1 and GPX4 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1964835, "gene_symbol_1": "REPS1", "gene_symbol_2": "GPX4", "detection_method": null}} +{"question_id": "PPIL1-1148", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: COL24A1 (Q17RW2)\n Function: May participate in regulating type I collagen fibrillogenesis at specific anatomical locations during fetal development\n Location: Secreted, extracellular space, extracellular matrix\n\nProtein 2: ANKS6 (Q68DC2)\n Function: Required for renal function\n Location: Cell projection, cilium\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between COL24A1 and ANKS6.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2069488, "gene_symbol_1": "COL24A1", "gene_symbol_2": "ANKS6", "detection_method": null}} +{"question_id": "PPIL1-1149", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: POLE (Q07864)\n Function: Catalytic component of the DNA polymerase epsilon complex (PubMed:10801849). Participates in chromosomal DNA replication (By similarity). Required during synthesis of the leading DNA strands at the re\n Location: Nucleus\n\nProtein 2: ANKRD52 (Q8NB46)\n Function: Putative regulatory subunit of protein phosphatase 6 (PP6) that may be involved in the recognition of phosphoprotein substrates\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between POLE and ANKRD52.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2030048, "gene_symbol_1": "POLE", "gene_symbol_2": "ANKRD52", "detection_method": null}} +{"question_id": "PPIL1-1150", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: FGD2 (Q7Z6J4)\n Function: Activates CDC42, a member of the Ras-like family of Rho- and Rac proteins, by exchanging bound GDP for free GTP. Activates JNK1 via CDC42 but not RAC1. Binds to phosphatidylinositol 4,5-bisphosphate, \n Location: Cytoplasm, cytoskeleton\n\nProtein 2: OLFML2A (Q68BL7)\n Location: Secreted\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between FGD2 and OLFML2A.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2105231, "gene_symbol_1": "FGD2", "gene_symbol_2": "OLFML2A", "detection_method": null}} +{"question_id": "PPIL1-1151", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: EIF3D (O15371)\n Function: mRNA cap-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, a complex required for several steps in the initiation of protein synthesis of a specialized repertoire of\n Location: Cytoplasm\n\nProtein 2: ZNF716 (A6NP11)\n Function: May be involved in transcriptional regulation\n Location: Nucleus\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between EIF3D and ZNF716 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1756776, "gene_symbol_1": "EIF3D", "gene_symbol_2": "ZNF716", "detection_method": null}} +{"question_id": "PPIL1-1152", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: MAGEA6 (P43360)\n Function: Activator of ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases that acts as a repressor of autophagy (PubMed:17942928, PubMed:20864041, PubMed:31267705). May e\n\nProtein 2: MAGEB5 (Q9BZ81)\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between MAGEA6 and MAGEB5.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1972894, "gene_symbol_1": "MAGEA6", "gene_symbol_2": "MAGEB5", "detection_method": null}} +{"question_id": "PPIL1-1153", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: TACR1 (P25103)\n Function: Receptor for the tachykinin substance P, also able to bind and respond to tachynins neurokinin A/substance K and neurokinin B/neuromedin-K (PubMed:1718267, PubMed:15452552). The rank order of affinity\n Location: Cell membrane\n\nProtein 2: PCDHA11 (Q9Y5I1)\n Function: Potential calcium-dependent cell-adhesion protein. May be involved in the establishment and maintenance of specific neuronal connections in the brain\n Location: Cell membrane\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between TACR1 and PCDHA11 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1933629, "gene_symbol_1": "TACR1", "gene_symbol_2": "PCDHA11", "detection_method": null}} +{"question_id": "PPIL1-1154", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: IPO4 (Q8TEX9)\n Function: Nuclear transport receptor that mediates nuclear import of proteins, such as histones, RPS3A, TNP2 and VDR (PubMed:11823430, PubMed:16207705, PubMed:17682055, PubMed:21454524, PubMed:30177573). Serves\n Location: Cytoplasm\n\nProtein 2: POTEC (B2RU33)\n\nEvidence: No significant association between IPO4 and POTEC was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1763365, "gene_symbol_1": "IPO4", "gene_symbol_2": "POTEC", "detection_method": null}} +{"question_id": "PPIL1-1155", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: SPATA46 (Q5T0L3)\n Function: Plays a role in spermiogenesis and fertilization\n Location: Nucleus membrane\n\nProtein 2: NOTCH2 (Q04721)\n Function: Functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellu\n Location: Cell membrane\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between SPATA46 and NOTCH2 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2026287, "gene_symbol_1": "SPATA46", "gene_symbol_2": "NOTCH2", "detection_method": null}} +{"question_id": "PPIL1-1156", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: TSC22D4 (Q9Y3Q8)\n Function: Binds DNA and acts as a transcriptional repressor (PubMed:10488076). Involved in the regulation of systematic glucose homeostasis and insulin sensitivity, via transcriptional repression of downstream \n Location: Nucleus\n\nProtein 2: TMEM139 (Q8IV31)\n Function: May be involved in cellular trafficking of proteins such as SLC4A1\n Location: Membrane\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between TSC22D4 and TMEM139 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2151635, "gene_symbol_1": "TSC22D4", "gene_symbol_2": "TMEM139", "detection_method": null}} +{"question_id": "PPIL1-1157", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: PROM1 (O43490)\n Function: May play a role in cell differentiation, proliferation and apoptosis (PubMed:24556617). Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization\n Location: Apical cell membrane\n\nProtein 2: GCNA (Q96QF7)\n Function: May play a role in DNA-protein cross-links (DPCs) clearance through a SUMO-dependent recruitment to sites of DPCs, ensuring the genomic stability by protecting germ cells and early embryos from variou\n Location: Nucleus\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between PROM1 and GCNA.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1813546, "gene_symbol_1": "PROM1", "gene_symbol_2": "GCNA", "detection_method": null}} +{"question_id": "PPIL1-1158", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: SANBR (Q6NSI8)\n Function: Negatively regulates class switch recombination or isotype switching in splenic B-cells\n\nProtein 2: TMC2 (Q8TDI7)\n Function: Pore-forming subunit of the mechanotransducer (MET) non-selective cation channel complex located at the tips of stereocilia of cochlear hair cells and that mediates sensory transduction in the auditor\n Location: Cell membrane\n\nEvidence: No significant association between SANBR and TMC2 was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2111655, "gene_symbol_1": "SANBR", "gene_symbol_2": "TMC2", "detection_method": null}} +{"question_id": "PPIL1-1159", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: FGD1 (P98174)\n Function: Activates CDC42, a member of the Ras-like family of Rho- and Rac proteins, by exchanging bound GDP for free GTP. Plays a role in regulating the actin cytoskeleton and cell shape\n Location: Cytoplasm\n\nProtein 2: CAPN14 (A8MX76)\n Function: Calcium-regulated non-lysosomal thiol-protease\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between FGD1 and CAPN14.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1761255, "gene_symbol_1": "FGD1", "gene_symbol_2": "CAPN14", "detection_method": null}} +{"question_id": "PPIL1-1160", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: STXBP6 (Q8NFX7)\n Function: Forms non-fusogenic complexes with SNAP25 and STX1A and may thereby modulate the formation of functional SNARE complexes and exocytosis\n Location: Cytoplasm\n\nProtein 2: FILIP1 (Q7Z7B0)\n Function: By acting through a filamin-A/F-actin axis, it controls the start of neocortical cell migration from the ventricular zone. May be able to induce the degradation of filamin-A\n Location: Cytoplasm, cytoskeleton\n\nEvidence: No significant association between STXBP6 and FILIP1 was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2140272, "gene_symbol_1": "STXBP6", "gene_symbol_2": "FILIP1", "detection_method": null}} +{"question_id": "PPIL1-1161", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: MAPK8IP2 (Q13387)\n Function: The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. JIP2 i\n Location: Cytoplasm\n\nProtein 2: OLAH (Q5VUC1)\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between MAPK8IP2 and OLAH.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2041871, "gene_symbol_1": "MAPK8IP2", "gene_symbol_2": "OLAH", "detection_method": null}} +{"question_id": "PPIL1-1162", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: MEDAG (Q5VYS4)\n Function: Involved in processes that promote adipocyte differentiation, lipid accumulation, and glucose uptake in mature adipocytes\n Location: Cytoplasm\n\nProtein 2: PGC (P20142)\n Function: Hydrolyzes a variety of proteins\n Location: Secreted\n\nEvidence: No significant association between MEDAG and PGC was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1922205, "gene_symbol_1": "MEDAG", "gene_symbol_2": "PGC", "detection_method": null}} +{"question_id": "PPIL1-1163", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: FPGS (Q05932)\n Function: Catalyzes conversion of folates to polyglutamate derivatives allowing concentration of folate compounds in the cell and the intracellular retention of these cofactors, which are important substrates f\n Location: Mitochondrion inner membrane\n\nProtein 2: ACTR3C (Q9C0K3)\n Function: May play a role in the suppression of metastatic potential in lung adenoma carcinoma cells\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between FPGS and ACTR3C.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2027711, "gene_symbol_1": "FPGS", "gene_symbol_2": "ACTR3C", "detection_method": null}} +{"question_id": "PPIL1-1164", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: MELTF (P08582)\n Function: Involved in iron cellular uptake. Seems to be internalized and then recycled back to the cell membrane. Binds a single atom of iron per subunit. Could also bind zinc\n Location: Cell membrane\n\nProtein 2: PRSS57 (Q6UWY2)\n Function: Serine protease that cleaves preferentially after Arg residues (PubMed:22474388, PubMed:23904161, PubMed:25156428). Can also cleave after citrulline (deimidated arginine) and methylarginine residues (\n Location: Cytoplasmic granule lumen\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between MELTF and PRSS57.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1886430, "gene_symbol_1": "MELTF", "gene_symbol_2": "PRSS57", "detection_method": null}} +{"question_id": "PPIL1-1165", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: SLC35B2 (Q8TB61)\n Function: Probably functions as a 3'-phosphoadenylyl sulfate:adenosine 3',5'-bisphosphate antiporter at the Golgi membranes. Mediates the transport from the cytosol into the lumen of the Golgi of 3'-phosphoaden\n Location: Golgi apparatus membrane\n\nProtein 2: TTLL2 (Q9BWV7)\n Function: Probable tubulin polyglutamylase that generates side chains of glutamate on the gamma-carboxyl group of specific glutamate residues within the C-terminal tail of target proteins (By similarity). Simil\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between SLC35B2 and TTLL2 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2182662, "gene_symbol_1": "SLC35B2", "gene_symbol_2": "TTLL2", "detection_method": null}} +{"question_id": "PPIL1-1166", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: GMPR (P36959)\n Function: Catalyzes the irreversible NADPH-dependent deamination of GMP to IMP. It functions in the conversion of nucleobase, nucleoside and nucleotide derivatives of G to A nucleotides, and in maintaining the \n\nProtein 2: LRRC72 (A6NJI9)\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between GMPR and LRRC72.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1752276, "gene_symbol_1": "GMPR", "gene_symbol_2": "LRRC72", "detection_method": null}} +{"question_id": "PPIL1-1167", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: PDE8B (O95263)\n Function: Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in specific signaling in the thyroid gland\n\nProtein 2: PARN (O95453)\n Function: 3'-exoribonuclease that has a preference for poly(A) tails of mRNAs, thereby efficiently degrading poly(A) tails. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of\n Location: Nucleus\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between PDE8B and PARN is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1855190, "gene_symbol_1": "PDE8B", "gene_symbol_2": "PARN", "detection_method": null}} +{"question_id": "PPIL1-1168", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: HMGB3 (O15347)\n Function: Multifunctional protein with various roles in different cellular compartments. May act in a redox sensitive manner. Associates with chromatin and binds DNA with a preference for non-canonical DNA stru\n Location: Nucleus\n\nProtein 2: SLC9A5 (Q14940)\n Function: Plasma membrane Na(+)/H(+) antiporter. Mediates the electroneutral exchange of intracellular H(+) ions for extracellular Na(+) in 1:1 stoichiometry, thus regulating intracellular pH homeostasis, in pa\n Location: Cell membrane\n\nEvidence: No significant association between HMGB3 and SLC9A5 was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1804979, "gene_symbol_1": "HMGB3", "gene_symbol_2": "SLC9A5", "detection_method": null}} +{"question_id": "PPIL1-1169", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: RRM2 (P31350)\n Function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. Inhibits Wnt signaling\n Location: Cytoplasm\n\nProtein 2: ANXA2R (Q3ZCQ2)\n Function: May act as a receptor for annexin II on marrow stromal cells to induce osteoclast formation\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between RRM2 and ANXA2R is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1944848, "gene_symbol_1": "RRM2", "gene_symbol_2": "ANXA2R", "detection_method": null}} +{"question_id": "PPIL1-1170", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: WDR4 (P57081)\n Function: Non-catalytic component of the METTL1-WDR4 methyltransferase complex required for the formation of N(7)-methylguanine in a subset of RNA species, such as tRNAs, mRNAs and microRNAs (miRNAs) (PubMed:12\n Location: Nucleus\n\nProtein 2: ITGAV (P06756)\n Function: The alpha-V (ITGAV) integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. T\n Location: Cell membrane\n\nEvidence: No significant association between WDR4 and ITGAV was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1881662, "gene_symbol_1": "WDR4", "gene_symbol_2": "ITGAV", "detection_method": null}} +{"question_id": "PPIL1-1171", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: SMAD9 (O15198)\n Function: Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD9 is a receptor-regulated SMAD (R-SMAD)\n Location: Cytoplasm\n\nProtein 2: SYT11 (Q9BT88)\n Function: Synaptotagmin family member involved in vesicular and membrane trafficking which does not bind Ca(2+). Inhibits clathrin-mediated and bulk endocytosis, functions to ensure precision in vesicle retriev\n Location: Cytoplasmic vesicle membrane\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between SMAD9 and SYT11.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1802791, "gene_symbol_1": "SMAD9", "gene_symbol_2": "SYT11", "detection_method": null}} +{"question_id": "PPIL1-1172", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: BAG2 (O95816)\n Function: Co-chaperone for HSP70 and HSC70 chaperone proteins. Acts as a nucleotide-exchange factor (NEF) promoting the release of ADP from the HSP70 and HSC70 proteins thereby triggering client/substrate prote\n\nProtein 2: TSC22D3 (Q99576)\n Function: Protects T-cells from IL2 deprivation-induced apoptosis through the inhibition of FOXO3A transcriptional activity that leads to the down-regulation of the pro-apoptotic factor BCL2L11 (PubMed:15031210\n Location: Cytoplasm\n\nEvidence: No significant association between BAG2 and TSC22D3 was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1862293, "gene_symbol_1": "BAG2", "gene_symbol_2": "TSC22D3", "detection_method": null}} +{"question_id": "PPIL1-1173", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: TEX101 (Q9BY14)\n Function: Plays a role in fertilization by controlling binding of sperm to zona pellucida and migration of spermatozoa into the oviduct (By similarity). May play a role in signal transduction and promote protei\n Location: Cell membrane\n\nProtein 2: WNT16 (Q9UBV4)\n Function: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is\n Location: Secreted, extracellular space, extracellular matrix\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between TEX101 and WNT16 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2221768, "gene_symbol_1": "TEX101", "gene_symbol_2": "WNT16", "detection_method": null}} +{"question_id": "PPIL1-1174", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: INTU (Q9ULD6)\n Function: Plays a key role in ciliogenesis and embryonic development. Regulator of cilia formation by controlling the organization of the apical actin cytoskeleton and the positioning of the basal bodies at the\n Location: Cytoplasm\n\nProtein 2: TULP1 (O00294)\n Function: Required for normal development of photoreceptor synapses. Required for normal photoreceptor function and for long-term survival of photoreceptor cells. Interacts with cytoskeleton proteins and may pl\n Location: Cytoplasm\n\nEvidence: No significant association between INTU and TULP1 was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1785541, "gene_symbol_1": "INTU", "gene_symbol_2": "TULP1", "detection_method": null}} +{"question_id": "PPIL1-1175", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: GAD1 (Q99259)\n Function: Catalyzes the synthesis of the inhibitory neurotransmitter gamma-aminobutyric acid (GABA) with pyridoxal 5'-phosphate as cofactor\n\nProtein 2: PAFAH2 (Q99487)\n Function: Catalyzes the hydrolyze of the acetyl group at the sn-2 position of platelet-activating factor (PAF) and its analogs, leading to their inactivation (PubMed:9494101). Hydrolyzes propionyl and butyroyl \n Location: Cytoplasm\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between GAD1 and PAFAH2 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2213756, "gene_symbol_1": "GAD1", "gene_symbol_2": "PAFAH2", "detection_method": null}} +{"question_id": "PPIL1-1176", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: PSMD7 (P51665)\n Function: Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by \n\nProtein 2: C11orf87 (Q6NUJ2)\n Location: Membrane\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between PSMD7 and C11orf87.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1988630, "gene_symbol_1": "PSMD7", "gene_symbol_2": "C11orf87", "detection_method": null}} +{"question_id": "PPIL1-1177", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: PLCG1 (P19174)\n Function: Mediates the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). Plays an important role in the regulation of intracellular signaling cascades. Be\n Location: Cell projection, lamellipodium\n\nProtein 2: SAXO1 (Q8IYX7)\n Function: May play a role in the regulation of cilium length. Stabilizes microtubules at low temperature\n Location: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole\n\nEvidence: No significant association between PLCG1 and SAXO1 was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1920084, "gene_symbol_1": "PLCG1", "gene_symbol_2": "SAXO1", "detection_method": null}} +{"question_id": "PPIL1-1178", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: TOX (O94900)\n Function: Transcriptional regulator with a major role in neural stem cell commitment and corticogenesis as well as in lymphoid cell development and lymphoid tissue organogenesis (By similarity). Binds to GC-ric\n Location: Nucleus\n\nProtein 2: PTPRN2 (Q92932)\n Function: Plays a role in vesicle-mediated secretory processes. Required for normal accumulation of secretory vesicles in hippocampus, pituitary and pancreatic islets. Required for the accumulation of normal le\n Location: Cytoplasmic vesicle, secretory vesicle membrane\n\nEvidence: No significant association between TOX and PTPRN2 was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1849685, "gene_symbol_1": "TOX", "gene_symbol_2": "PTPRN2", "detection_method": null}} +{"question_id": "PPIL1-1179", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: CIMAP1D (Q3SX64)\n\nProtein 2: FBXO3 (Q9UK99)\n Function: Substrate recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex, SCF(FBXO3), which mediates the ubiquitination and subsequent proteasomal degradation of target pr\n Location: Nucleus\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between CIMAP1D and FBXO3.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2076820, "gene_symbol_1": "CIMAP1D", "gene_symbol_2": "FBXO3", "detection_method": null}} +{"question_id": "PPIL1-1180", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: KIF5C (O60282)\n Function: Microtubule-associated force-producing protein that may play a role in organelle transport. Has ATPase activity (By similarity). Involved in synaptic transmission (PubMed:24812067). Mediates dendritic\n Location: Cytoplasm, cytoskeleton\n\nProtein 2: TNFSF13 (O75888)\n Function: Cytokine that binds to TNFRSF13B/TACI and to TNFRSF17/BCMA. Plays a role in the regulation of tumor cell growth. May be involved in monocyte/macrophage-mediated immunological processes\n Location: Secreted\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between KIF5C and TNFSF13 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1821463, "gene_symbol_1": "KIF5C", "gene_symbol_2": "TNFSF13", "detection_method": null}} +{"question_id": "PPIL1-1181", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: NEK10 (Q6ZWH5)\n Function: Plays a role in the cellular response to UV irradiation. Mediates G2/M cell cycle arrest, MEK autoactivation and ERK1/2-signaling pathway activation in response to UV irradiation. In ciliated cells of\n\nProtein 2: ANKRD33B (A6NCL7)\n\nEvidence: No significant association between NEK10 and ANKRD33B was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1744693, "gene_symbol_1": "NEK10", "gene_symbol_2": "ANKRD33B", "detection_method": null}} +{"question_id": "PPIL1-1182", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: LRRC8C (Q8TDW0)\n Function: Non-essential component of the volume-regulated anion channel (VRAC, also named VSOAC channel), an anion channel required to maintain a constant cell volume in response to extracellular or intracellul\n Location: Cell membrane\n\nProtein 2: OR10H4 (Q8NGA5)\n Function: Odorant receptor\n Location: Cell membrane\n\nEvidence: No significant association between LRRC8C and OR10H4 was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2178047, "gene_symbol_1": "LRRC8C", "gene_symbol_2": "OR10H4", "detection_method": null}} +{"question_id": "PPIL1-1183", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: CKLF-CMTM1 (A0A087WVB3)\n Location: Membrane\n\nProtein 2: CD248 (Q9HCU0)\n Function: Cell surface glycoprotein involved in various biological processes including angiogenesis, immune response modulation, and tissue remodeling and repair. Participates in pericyte proliferation through \n Location: Membrane\n\nEvidence: No significant association between CKLF-CMTM1 and CD248 was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1730719, "gene_symbol_1": "CKLF-CMTM1", "gene_symbol_2": "CD248", "detection_method": null}} +{"question_id": "PPIL1-1184", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: TSNARE1 (Q96NA8)\n Location: Membrane\n\nProtein 2: CYP2E1 (P05181)\n Function: A cytochrome P450 monooxygenase involved in the metabolism of fatty acids (PubMed:10553002, PubMed:18577768). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and red\n Location: Endoplasmic reticulum membrane\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between TSNARE1 and CYP2E1.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1879045, "gene_symbol_1": "TSNARE1", "gene_symbol_2": "CYP2E1", "detection_method": null}} +{"question_id": "PPIL1-1185", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: FER1L6 (Q2WGJ9)\n Function: Calcium/phospholipid-binding protein which is essential for proper development of skeletal and cardiac muscle\n Location: Cell membrane\n\nProtein 2: GPC5 (P78333)\n Function: Cell surface proteoglycan that bears heparan sulfate\n Location: Cell membrane\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between FER1L6 and GPC5 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2013829, "gene_symbol_1": "FER1L6", "gene_symbol_2": "GPC5", "detection_method": null}} +{"question_id": "PPIL1-1186", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: EXOC1 (Q9NV70)\n Function: Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane\n Location: Midbody, Midbody ring\n\nProtein 2: INSM2 (Q96T92)\n Function: May function as a growth suppressor or tumor suppressor in liver cells and in certain neurons\n Location: Cytoplasm\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between EXOC1 and INSM2 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2213638, "gene_symbol_1": "EXOC1", "gene_symbol_2": "INSM2", "detection_method": null}} +{"question_id": "PPIL1-1187", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: LEP (P41159)\n Function: Key player in the regulation of energy balance and body weight control. Once released into the circulation, has central and peripheral effects by binding LEPR, found in many tissues, which results in \n Location: Secreted\n\nProtein 2: RNF182 (Q8N6D2)\n Function: E3 ubiquitin-protein ligase that mediates the ubiquitination of ATP6V0C and targets it to degradation via the ubiquitin-proteasome pathway (PubMed:18298843). Also plays a role in the inhibition of TLR\n Location: Membrane\n\nEvidence: No significant association between LEP and RNF182 was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1968022, "gene_symbol_1": "LEP", "gene_symbol_2": "RNF182", "detection_method": null}} +{"question_id": "PPIL1-1188", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: ITGBL1 (O95965)\n Location: Secreted\n\nProtein 2: TTF1 (Q15361)\n Function: Multifunctional nucleolar protein that terminates ribosomal gene transcription, mediates replication fork arrest and regulates RNA polymerase I transcription on chromatin. Plays a dual role in rDNA re\n Location: Nucleus\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between ITGBL1 and TTF1.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1864528, "gene_symbol_1": "ITGBL1", "gene_symbol_2": "TTF1", "detection_method": null}} +{"question_id": "PPIL1-1189", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: RLF (Q13129)\n Function: May be involved in transcriptional regulation\n Location: Nucleus\n\nProtein 2: PRAG1 (Q86YV5)\n Function: Catalytically inactive protein kinase that acts as a scaffold protein. Functions as an effector of the small GTPase RND2, which stimulates RhoA activity and inhibits NGF-induced neurite outgrowth (By \n Location: Cytoplasm\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between RLF and PRAG1 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2039123, "gene_symbol_1": "RLF", "gene_symbol_2": "PRAG1", "detection_method": null}} +{"question_id": "PPIL1-1190", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: PRDX1 (Q06830)\n Function: Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by \n Location: Cytoplasm\n\nProtein 2: CATSPERT (Q53TS8)\n Function: Auxiliary component of the CatSper complex, a complex involved in sperm cell hyperactivation. Sperm cell hyperactivation is needed for sperm motility which is essential late in the preparation of sper\n Location: Cell projection, cilium, flagellum membrane\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between PRDX1 and CATSPERT is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2029024, "gene_symbol_1": "PRDX1", "gene_symbol_2": "CATSPERT", "detection_method": null}} +{"question_id": "PPIL1-1191", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: BKGD (Q9H0W9)\n Function: Catalyzes the decarboxylation of 3-dehydro-L-gulonate to produce L-xylulose, used in the pentose pathway (PubMed:40737316). Exhibits ester hydrolase activity on p-nitrophenyl acetate, in vitro (PubMed\n Location: Nucleus\n\nProtein 2: VCPIP1 (Q96JH7)\n Function: Deubiquitinating enzyme involved in DNA repair and reassembly of the Golgi apparatus and the endoplasmic reticulum following mitosis (PubMed:32649882). Necessary for VCP-mediated reassembly of Golgi s\n Location: Nucleus\n\nEvidence: No significant association between BKGD and VCPIP1 was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2206485, "gene_symbol_1": "BKGD", "gene_symbol_2": "VCPIP1", "detection_method": null}} +{"question_id": "PPIL1-1192", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: GAREM1 (Q9H706)\n Function: Acts as an adapter protein that plays a role in intracellular signaling cascades triggered either by the cell surface activated epidermal growth factor receptor and/or cytoplasmic protein tyrosine kin\n\nProtein 2: TPSB2 (A0A087WUI4)\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between GAREM1 and TPSB2 is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1730417, "gene_symbol_1": "GAREM1", "gene_symbol_2": "TPSB2", "detection_method": null}} +{"question_id": "PPIL1-1193", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: GJA8 (P48165)\n Function: Structural component of eye lens gap junctions (PubMed:18006672, PubMed:19756179). Gap junctions are dodecameric channels that connect the cytoplasm of adjoining cells. They are formed by the docking \n Location: Cell membrane\n\nProtein 2: BPIFB6 (Q8NFQ5)\n Location: Secreted\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between GJA8 and BPIFB6.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1978132, "gene_symbol_1": "GJA8", "gene_symbol_2": "BPIFB6", "detection_method": null}} +{"question_id": "PPIL1-1194", "task": "ppi-l1", "split": "test", "difficulty": "easy", "context_text": "Protein 1: NFE2L1 (Q14494)\n Function: Endoplasmic reticulum membrane sensor that translocates into the nucleus in response to various stresses to act as a transcription factor (PubMed:20932482, PubMed:24448410). Constitutes a precursor of\n Location: Endoplasmic reticulum membrane\n\nProtein 2: UQCRQ (O14949)\n Function: Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation. The resp\n Location: Mitochondrion inner membrane\n\nEvidence: Across all available evidence channels (experimental, database, text-mining, co-expression, co-occurrence, gene fusion, neighborhood), the combined interaction score between NFE2L1 and UQCRQ is zero or negligible.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1798612, "gene_symbol_1": "NFE2L1", "gene_symbol_2": "UQCRQ", "detection_method": null}} +{"question_id": "PPIL1-1195", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: SHBG (P04278)\n Function: Functions as an androgen transport protein, but may also be involved in receptor mediated processes. Each dimer binds one molecule of steroid. Specific for 5-alpha-dihydrotestosterone, testosterone, a\n Location: Secreted\n\nProtein 2: NLRP12 (P59046)\n Function: Plays an essential role as an potent mitigator of inflammation (PubMed:30559449). Primarily expressed in dendritic cells and macrophages, inhibits both canonical and non-canonical NF-kappa-B and ERK a\n Location: Cytoplasm\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between SHBG and NLRP12.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1876302, "gene_symbol_1": "SHBG", "gene_symbol_2": "NLRP12", "detection_method": null}} +{"question_id": "PPIL1-1196", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: PLP2 (Q04941)\n Function: May play a role in cell differentiation in the intestinal epithelium\n Location: Membrane\n\nProtein 2: ANKRD34C (P0C6C1)\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between PLP2 and ANKRD34C.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1891976, "gene_symbol_1": "PLP2", "gene_symbol_2": "ANKRD34C", "detection_method": null}} +{"question_id": "PPIL1-1197", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: C9orf57 (Q5W0N0)\n Location: Membrane\n\nProtein 2: ZRANB3 (Q5FWF4)\n Function: DNA annealing helicase and endonuclease required to maintain genome stability at stalled or collapsed replication forks by facilitating fork restart and limiting inappropriate recombination that could\n Location: Nucleus\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between C9orf57 and ZRANB3.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2085937, "gene_symbol_1": "C9orf57", "gene_symbol_2": "ZRANB3", "detection_method": null}} +{"question_id": "PPIL1-1198", "task": "ppi-l1", "split": "test", "difficulty": "medium", "context_text": "Protein 1: SAMD11 (Q96NU1)\n Function: Component of a Polycomb group (PcG) multiprotein PRC1-like complex, essential for establishing rod photoreceptor cell identity and function by silencing nonrod gene expression in developing rod photor\n Location: Nucleus\n\nProtein 2: MRPL30 (B8ZZV5)\n\nEvidence: An aggregated database integrating multiple evidence types found no meaningful interaction signal between SAMD11 and MRPL30.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 1765761, "gene_symbol_1": "SAMD11", "gene_symbol_2": "MRPL30", "detection_method": null}} +{"question_id": "PPIL1-1199", "task": "ppi-l1", "split": "test", "difficulty": "hard", "context_text": "Protein 1: CCDC33 (Q8N5R6)\n\nProtein 2: PANX3 (Q96QZ0)\n Function: Regulator of osteoblast differentiation by functioning as a Ca(2+) channel in the endoplasmic reticulum which regulates calmodulin (CaM) pathways. Allows ATP release into the extracellular space and a\n Location: Cell membrane\n\nEvidence: No significant association between CCDC33 and PANX3 was found across available interaction evidence.", "gold_answer": "D", "gold_category": "database_absence", "metadata": {"source_db": "string", "confidence_tier": "bronze", "result_id": 2165926, "gene_symbol_1": "CCDC33", "gene_symbol_2": "PANX3", "detection_method": null}}