Rank	Athlete	Nationality	Time	Notes
1	Félix Sánchez	Dominican Republic	47.76	Q, SB
2	Jehue Gordon	Trinidad and Tobago	47.96	Q, NR
3	Kerron Clement	United States	48.12	q, SB
4	Dai Greene	Great Britain	48.19	q
5	Mamadou Kasse Hanne	Senegal	48.80	PB
6	Michael Bultheel	Belgium	49.10	PB
7	Eric Alejandro	Puerto Rico	49.15	-
8	Kurt Couto	Mozambique	51.55	-